The invention discloses an intelligent biological sample transport case which comprises a heat preservation transport case body. A case door is hinged to one side of the heat preservation transport case body through a hinge, an intelligent coded lock is arranged on the side, away from the hinge, of the heat preservation transport case body and the case door, and temperature control probes are fixedly installed on the two sides of the bottom of the case door; and a supporting plate is arranged in the heat preservation transportation case, a plurality of fixing holes are formed in the supporting plate, protection structures are arranged in the fixing holes, a fixing seat is arranged below the supporting plate, a fixing mechanism is arranged on one side of the heat preservation transportation box, a liquid nitrogen tank is arranged on the fixing mechanism, and a GPS positioner is further arranged on one side of the heat preservation transportation case. Long-time transportation of biological samples is facilitated, it is guaranteed that the position of the biological samples and the temperature in the case can be monitored at any time in the transportation process, real-time records can be remotely stored and printed at any time, the transportation safety is improved, meanwhile, the working efficiency is improved, and the biological hazard risk is reduced.