The application discloses a micro-fluidic
chip reagent injection device, which comprises a
liquid storage part with at least one quantitative sealed container, a flexible pressure-bearing membrane arranged on the top of the container body of the quantitative sealed container, a hard flow guide column arranged on the bottom of the container body, and a sealing membrane arranged on the liquid outlet; a liquid inlet part comprising a flow column and a
liquid storage pool arranged on the micro-fluidic
chip, the inner cavity of the flow column is matched with the outer
diameter of the hard flow guide column, a gas guide channel is arranged on the contact surface of the flow column and the hard flow guide column for connecting the inner cavity of the flow column with the external environment, and a puncture part opposite to the sealing membrane is arranged on the flow column; wherein the hard flow guide column has a first plug-in position for making the puncture part puncture the sealing membrane when the hard flow guide column is pressed down. The device has the advantages of simple structure, low cost, convenient use, accurate quantitative injection and
biological hazard prevention. The application also discloses a micro-fluidic
chip reagent injection method.