The invention discloses CTL epitope peptide of HTNV-Gn / Gc specificity limited by HLA-A*02, as well as application thereof. The nucleotide sequence of the CTL epitope peptide is shown in SEQ ID No: 1-3. The CTL epitope peptide of HTNV-Gn / Gc specificity is epitope polypeptide limited by HLA-A*02 molecule, can induce the CD8+T cell to generate strong cell immune response, excrete IFN-gamma; the frequency of excreting IFN-gamma can be used for indicating the severity degree of an HFRS patient, i.e., the higher the frequency of excreting IFN-gamma is, the less severe the HFRS condition is; therefore, the CTL epitope peptide can be applied to the manufacturing of CTL epitope peptide vaccine, inducing of cytotoxicity T cell capable of generating CTL epitope peptide specificity, or manufacturing of HLA-A*02 / epitope peptide-compound tetramer, and has excellent development and application prospect in the field of immunological therapy of HFRS specificity.