The invention discloses TAT-LBD-PEP 
fusion protein and application of the TAT-LBD-PEP 
fusion protein in treatment of 
central nervous system lesion. The TAT-LBD-PEP 
fusion protein is combined with 
myelin inhibiting factors MAG, Nogo-66 and OMgp, accordingly the 
axon regenerative capacity is promoted through 
antagonism PirB effect, and the TAT-LBD-PEP fusion 
protein has the advantages of being high transduction efficiency and easily transmitting a blood-spinal barrier and a 
blood brain barrier, can transduce targeting to a hurt region and has the enrichment effect on an 
ischemic region. The problems that additional harm of nervous tissues is caused by the 
protein injection in a micro-injection mode or other injection 
modes and 
macromolecule cannot transmit the blood-spinal barrier and the 
blood brain barrier are solved, and the shortcoming that corresponding biological concentration cannot be met is overcome, and the targeting is transduced to the 
ischemic region through a 
ligand binding domain (LBD). The TAT-LBD-PEP fusion 
protein can be prepared in large-scale mode, is low in cost and high in activity, and is used for treatment of various brain and 
spinal cord injury including cerebral 
ischemia hypoxia, cerebral hemorrhage, 
cerebral trauma, 
spinal cord injury and the like and promoting regeneration of nervous tissues and neural 
functional recovery.