17 results about "Cyclic nucleotide" patented technology

Disclosed herein, inter alia, are acyclic nucleotide analogs and methods of using an acyclic nucleotide analog for treating and / or ameliorating a papillomavirus infection.

Nucleic acid molecules comprising a first sequence encoding a cyclic nucleotide gated potassium channel or a functional fragment thereof and a second sequence encoding a chemogenetically activatable cyclic adenosine monophosphate (cAMP) generating receptor or a functional fragment thereof are provided. Expression vectors comprising the molecules, fusion proteins encoded by the molecules, cells, kits and pharmaceutical compositions are also provided. Methods of hyperpolarizing a cell, depolarizing a cardiac cell and treating a disease or condition are also provided.

The present disclosure is directed to blood-brain barrier penetrant channel inhibitors and their use in the treatment of major depressive disorders and other diseases. Patients with major depressive disorder exhibit alterations in their dopamine signaling pathways through the midbrain ventral tegmental area and a similar observation is also detected in preclinical models of stress - mice exhibit behavioral and physiological impairments following chronic social defeat stress. Prior studies have shown chronic social defeat stress-susceptible mice have increased ventral tegmental area dopamine neuronal excitability in part driven by an upregulation in hyperpolarization activated, cyclic nucleotide gated, Na+ / K+ (HCN) channels. Inhibiting these channels with known inhibitors such as Cilobradine has been shown to alleviate the negative behavioral effects of chronic social defeat stress.

The present invention relates to methods of improving abiotic stress tolerance, and specifically, maintaining the fertility of plants under stress, and in particular heat stress. Such methods comprise introducing one or more mutations into at least one nuclear localized cyclic nucleotide-gated ion channel (CNGC) gene.

The application belongs to the technical field of plant genetic engineering, and particularly relates to a cyclic nucleotide-gated ion channel OsCNGC10 gene for regulating rice lodging resistance and drought resistance and application. A cyclic nucleotide-gated ion channel OsCNGC10 gene induced by drought is screened, and the nucleotide sequence is shown as SEQ ID NO:1; the protein sequence coded by the gene is shown as SEQ ID NO:2. An OsCNGC10 overexpression strain and a CRISPR / Cas9 mutant strain are obtained by using an agrobacterium-mediated transformation. Stem characteristics and lodging resistance analysis of the transgenic plants show that the mutant oscngc10-2 has enhanced stem strength and lodging resistance; overexpression of OsCNGC10 reduces the stem wall thickness and lignin content, and knockout of OsCNGC10 increases the stem lignin content, which preliminarily indicates that OsCNGC10 negatively regulates the rice lodging resistance. The results of the drought stress experiment in the seedling stage show that the MDA accumulation speed in the OsCNGC10 gene-deficient plants is accelerated, and the plants cannot form sufficient free proline; the free Pro content in the overexpression OsCNGC10 plants is greatly increased, and the MDA accumulation speed is relatively slow, which indicates that OsCNGC10 positively regulates the rice seedling drought resistance.

Disclosed herein are methods and compositions for modulating a bioelectric pattern in a subject, organ, cell, cell collective, or tissue in need thereof. In some embodiments, the modulation results in increasing the health and / or lifespan of a subject and / or has a therapeutic effect on a disease or condition that the subject is suffering from. In some embodiments, the bioelectric pattern is modulated by modulating the expression and / or activity of the hyperpolarization activate cyclic nucleotide gated potassium and sodium channel 2 (HCN2).

The application discloses application of VvNRT1.1 and VvCNGC15 proteins in improving nitrogen utilization rate of grape, and belongs to the technical field of plant genetic engineering and molecular breeding. The application proves that the VvNRT1.1 and VvCNGC15 proteins directly physically interact, and overexpression of the VvNRT1.1 or VvCNGC15 can improve the absorption and assimilation efficiency of nitrate nitrogen of grape. Therefore, the VvNRT1.1 and VvCNGC15 can be used as key molecular targets for improving nitrogen utilization rate, and have potential application value.

The invention belongs to the field of biomedical treatment, and particularly relates to pyroptosis related circular RNA for detecting myocardial ischemia reperfusion injury and a detection method and application thereof, the small RNA is named as pyroptosis related circular RNA (PYRCR), and the nucleotide sequence is shown as SEQ ID NO: 1. According to the invention, a myocardial ischemia reperfusion injury model is constructed, an in-vitro experimental model is utilized, and continuous and potent protein expression can be generated in a target tissue through adenovirus wrapping overexpression PYRCR. Therefore, the overexpressed PYRCY can relieve pyroptosis and improve the cardiac function after myocardial ischemia-reperfusion injury, and a new thought and basis are provided for relieving myocardial ischemia-reperfusion injury and researching and developing targeted drugs.

Embodiments of the invention are directed to new equatorially modified polymer linked multimers of guanosine-3′, 5′-cyclic monophosphate (cGMP) analogues that inhibit the cGMP-signaling system. The invention is also directed to related monomeric compounds, which may serve as monomeric precursors of the multimers, and / or also show itself inhibitory activity and / or impact the inhibitory activity of the related multimers. The invention further relates to the use of such compounds as reagents for signal transduction research and as modulators of cyclic nucleotide-regulated binding proteins and isoenzymes thereof, and as ligands for affinity chromatography, for antibody production or for diagnostic applications, e.g., on chip surfaces.

The application belongs to the technical field of plant genetic engineering, and particularly relates to a cyclic nucleotide-gated ion channel OsCNGC10 gene for regulating salt tolerance of rice and application thereof. The application discloses application of the OsCNGC10 gene in salt stress tolerance of rice, wherein the nucleotide sequence of the OsCNGC10 gene is shown as SEQ ID NO. 1, and the OsCNGC10 gene negatively regulates salt tolerance of rice. Specifically, rice with overexpression of the OsCNGC10 gene is more sensitive to salt stress, and knockout of the OsCNGC10 gene improves the salt stress tolerance of rice. The application enriches the function of the cyclic nucleotide-gated ion channel protein OsCNGC10 in rice stress, and provides a new gene resource for breeding of salt-resistant rice.

Disclosed herein are methods and compositions for modulating a bioelectric pattern in a subject, organ, cell, cell collective, or tissue in need thereof. In some embodiments, the modulation results in increasing the health and / or lifespan of a subject and / or has a therapeutic effect on a disease or condition that the subject is suffering from. In some embodiments, the bioelectric pattern is modulated by modulating the expression and / or activity of the hyperpolarization activate cyclic nucleotide gated potassium and sodium channel 2 (HCN2).

Substituted benzoxazole and benzofuran chemical entities of formula (I): #imgabs0# wherein V, W, X, Y, Z and m have any of the values described herein, and compositions comprising such chemical entities; methods for preparing them; and their use in various methods, including metabolic and reaction kinetics studies; detection and imaging techniques; radiotherapy; and the treatment of one or more conditions, including neurological conditions, cognitive disorders, immune conditions and inflammatory conditions, as well as other conditions and diseases involving PDE7 or cyclic nucleotide signaling.

This invention relates to a soybean cyst nematode gene, Hg-tax-4, and its application in nematode control. This invention addresses the problems of environmental pollution and easy loss of resistance in existing chemical control methods for soybean cyst nematodes, particularly when combined with resistant varieties and non-host rotation methods. The nucleotide sequence of the soybean cyst nematode gene Hg-tax-4 is shown in SEQ ID NO: 1 in the sequence listing. The application of the dsRNA of the soybean cyst nematode gene Hg-tax-4 in nematode control is also discussed. The gene Hg-tax-4 is derived from the cyclic nucleotide-gated ion channel subunit of the soybean cyst nematode, and experimental results show that this gene is expressed in the cerci of second-instar larvae. The soybean cyst nematode gene Hg-tax-4 participates in regulating the infection and reproduction of the soybean cyst nematode on its host and can be used as a target gene for the development of drugs for the control of soybean cyst nematodes.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a rice cyclic nucleotide gated ion channel OsCNGC10 gene in regulation and control of rice seed germination. The invention discloses an application of an OsCNGC10 gene in regulation and control of rice seed germination. A nucleotide sequence of the OsCNGC10 gene is as shown in SEQ ID NO. 1. By constructing OsCNGC10 gene overexpression and knockout plants, the germination rates of seeds of the knockout plants at different time points are higher than those of wild and overexpression plants, and the germination rates of the overexpression plants are lower than those of the wild and knockout plants. Therefore, the germination capability of seed germination can be improved by inhibiting the expression of the OsCNGC10 gene.

Embodiments of the invention are directed to new polymer linked multimeric guanosine-3′,5′-cyclic monophosphate (cGMP) analogues that modulate the cGMP-signaling system, preferably having activating properties, and more preferably being activators of cGMP dependent protein kinase (PKG), and related monomeric precursors thereof. The invention is also directed to related monomeric compounds, which may also show modulating activity and / or may serve as monomeric precursors of the multimers. The invention further relates to the use of such compounds as reagents for signal transduction research and as modulators of cyclic nucleotide-regulated binding proteins and isoenzymes thereof, and as ligands for affinity chromatography, for antibody production or for diagnostic applications e.g. on chip surfaces.

Described are methods for treating cancers overexpressing hyperpolarization activated cyclic nucleotide-gated channel (HCN) isoforms HCN1, HCN2, HCN3, and / or HCN4. The cancers are treated by administering to a subject in need of treatment an effective amount of an HCN inhibitor. The HCN inhibitor can be an inhibitor of HCN1, HCN2, HCN3, or HCN4, or an inhibitor that inhibits any combination of HCN1, HCN2, HCN3, and HCN4. The described methods are useful for reducing tumor size, putting cancer in remission, and reducing or stopping proliferation of cancer stem cells.

The present invention relates to the field of molecular biology, and in particular to a cyclic nucleotide-gated cation channel gene in the desert plant Zygophyllum grandiflorum, its encoded protein and application. The present invention provides the nucleotide sequence and encoded amino acid sequence of the cyclic nucleotide-gated cation channel gene ZxCNGC1;2. In yeast heterologous expression systems and mammalian HEK293T cell heterologous expression systems, the protein can mediate cation transport, which not only provides a theoretical basis for the selection and breeding of new stress-resistant plant varieties, but also provides new gene resources for the cultivation of stress-resistant crops, and has broad application prospects.