4 results about "Cyclic nucleotide" patented technology

The application belongs to the technical field of plant genetic engineering, and particularly relates to a cyclic nucleotide-gated ion channel OsCNGC10 gene for regulating rice lodging resistance and drought resistance and application. A cyclic nucleotide-gated ion channel OsCNGC10 gene induced by drought is screened, and the nucleotide sequence is shown as SEQ ID NO:1; the protein sequence coded by the gene is shown as SEQ ID NO:2. An OsCNGC10 overexpression strain and a CRISPR / Cas9 mutant strain are obtained by using an agrobacterium-mediated transformation. Stem characteristics and lodging resistance analysis of the transgenic plants show that the mutant oscngc10-2 has enhanced stem strength and lodging resistance; overexpression of OsCNGC10 reduces the stem wall thickness and lignin content, and knockout of OsCNGC10 increases the stem lignin content, which preliminarily indicates that OsCNGC10 negatively regulates the rice lodging resistance. The results of the drought stress experiment in the seedling stage show that the MDA accumulation speed in the OsCNGC10 gene-deficient plants is accelerated, and the plants cannot form sufficient free proline; the free Pro content in the overexpression OsCNGC10 plants is greatly increased, and the MDA accumulation speed is relatively slow, which indicates that OsCNGC10 positively regulates the rice seedling drought resistance.

The application discloses application of VvNRT1.1 and VvCNGC15 proteins in improving nitrogen utilization rate of grape, and belongs to the technical field of plant genetic engineering and molecular breeding. The application proves that the VvNRT1.1 and VvCNGC15 proteins directly physically interact, and overexpression of the VvNRT1.1 or VvCNGC15 can improve the absorption and assimilation efficiency of nitrate nitrogen of grape. Therefore, the VvNRT1.1 and VvCNGC15 can be used as key molecular targets for improving nitrogen utilization rate, and have potential application value.

The application belongs to the technical field of plant genetic engineering, and particularly relates to a cyclic nucleotide-gated ion channel OsCNGC10 gene for regulating salt tolerance of rice and application thereof. The application discloses application of the OsCNGC10 gene in salt stress tolerance of rice, wherein the nucleotide sequence of the OsCNGC10 gene is shown as SEQ ID NO. 1, and the OsCNGC10 gene negatively regulates salt tolerance of rice. Specifically, rice with overexpression of the OsCNGC10 gene is more sensitive to salt stress, and knockout of the OsCNGC10 gene improves the salt stress tolerance of rice. The application enriches the function of the cyclic nucleotide-gated ion channel protein OsCNGC10 in rice stress, and provides a new gene resource for breeding of salt-resistant rice.

Disclosed herein are methods and compositions for modulating a bioelectric pattern in a subject, organ, cell, cell collective, or tissue in need thereof. In some embodiments, the modulation results in increasing the health and / or lifespan of a subject and / or has a therapeutic effect on a disease or condition that the subject is suffering from. In some embodiments, the bioelectric pattern is modulated by modulating the expression and / or activity of the hyperpolarization activate cyclic nucleotide gated potassium and sodium channel 2 (HCN2).