3 results about "Cell based assays" patented technology

In the field of cell-based assays, i.e. cellular assays, there is a need for improved cell culture retaining inserts and cell culture retaining insert assemblies. A cell culture retaining insert (10; 70; 90; 100), for retaining a three-dimensional biological sample in a cell culture vessel (102; 132), comprises an insert body (12; 104) that has a positioning formation (16) to in-use locate the cell culture retaining insert (10; 70; 90; 100) relative to a cell culture vessel (102; 132). The insert body (12; 104) also has a retention element receiving formation (20). The cell culture retaining insert (10; 70; 90; 100) additionally includes a retention element (30; 60; 72; 92; 114) which is movably coupled with the receiving formation (20) of the insert body (12;104), whereby a distal retention end (32) of the retention element (30; 60; 72; 92; 114) is freely movable away from and towards the insert body (12; 104), and whereby in-use the weight of the retention element (30; 60; 72; 92; 114) less a buoyant force equal to the weight of any fluid the retention element (30; 60; 72; 92; 114) displaces gives rise to the retention end (32) exerting a retention force on one or more said biological samples.

The method as disclosed herein utilizes a high throughput screening assay (GigaAssay) to produce a comprehensive mutation effect on gene activity (MEGA)-mutation activity profile (Map). The methods as disclosed herein can be used to assess the mutational effect for any gene, under any condition (e.g., drug treatment) with any assay in mammalian cells in culture. Thus, the methods provided herein can be utilized to discover and screen dominant negative variants, and provide a reliable solution to the problem of identifying unique pharmacologically active variants of proteins. Furthermore, the method provided herein can be integrated to cell-based assays to investigate disease pathology and test potential drugs.