20 results about "Plate reader" patented technology

Cas-cleavable reporter systems, CRISPR-Cas systems thereof, and methods of use thereof in CRISPR-Cas based diagnostics. Cas-cleavable reporter systems may generate a luminescent, fluorescent, or other detectable signal upon Cas-collateral cleavage of one or more reporter system components. CRISPR-Cas systems may comprise a Cas protein having collateral cleavage activity, guide molecules, and the Cas-cleavable reporter system. Cas-cleavable reporter systems may be a split luciferase reporter system, at least one element of which is bound to beads. For multiplexing, CRISPR-Cas systems may comprise a Cas-cleavable quenched reporter system, optionally a Cas-cleavable quenched fluorescent reporter system, a Cas protein having collateral cleavage activity, and target-specific guide molecules attached to color-coded beads. CRISPR-Cas systems may further comprise amplification reagents. Methods may apply said CRISPR-Cas systems to flow cell, well-plate, or other devices, and may measure detectable signals by microscopic, plate reader, or other methods.

The invention relates to an environment-friendly switch vacuum arc-extinguishing chamber contact temperature monitoring method and system based on surface acoustic wave array sensing and inversion, and belongs to the field of electrical monitoring. In order to solve the problem that in the prior art, non-intrusive and high-reliability monitoring of the contact temperature of the vacuum arc-extinguishing chamber is difficult to realize due to vacuum sealing and metal shielding, the technical scheme of the invention is as follows: an acoustic surface wave sensor array comprising three or more sensing nodes is constructed on the outer wall of the vacuum arc-extinguishing chamber, and the longitudinal temperature gradient distribution of the outer wall is captured in a lossless manner. A narrow-band flat-plate reader antenna is adopted to realize array signal parallel transmission with a high signal-to-noise ratio, and the steady-state temperature of an internal contact is accurately inverted through a pre-trained physical information neural network (PINN) model by utilizing multi-source data fusion and array characteristics. According to the method, a non-intrusive monitoring function from external sensing to internal deduction is realized, the problem that the temperature of the contact cannot be measured in a high-voltage closed environment is solved, and a key technical support is provided for safe operation of environment-friendly switch equipment.

The utility model discloses an elisa plate reader convenient to clean, which comprises an elisa plate reader main body, the elisa plate reader main body is provided with a control panel, a sample loading plate is mounted at a sample inlet of the elisa plate reader main body, a limiting plate is hinged to the sample loading plate, a limiting square groove is formed in the limiting plate, an elisa plate is placed in the limiting square groove, and the elisa plate is arranged in the limiting square groove. A cleaning assembly is arranged above the elisa plate and comprises a first module, a second module and a cleaning brush, the cleaning brush is connected to the second module in an up-down sliding mode, the second module is installed at the output end of the first module, the first module is installed on the microplate reader body, and the second module is installed at the output end of the second module. The sample loading plate and the limiting plate are automatically cleaned through the cleaning assembly, the cleaning efficiency is improved, the limiting plate is hinged to the sample loading plate, separate cleaning is facilitated, and cleaning is cleaner.

The present invention generally relates to a solid-state, multi-well plate reader including an emitter assembly having a plurality of emitters and a receptor assembly having a plurality of receptors, where the positions of these assemblies are not fixed relative to each other but are temporarily aligned for measurement by way of two alignment trays.

The invention provides a multi-well plate reader for providing simultaneous transmission of stimulation light to, and detection of emission light from, individual wells of a multi-well plate at a plurality of distinct wavelengths.

Protein biosensors for drug discovery, such as a high-throughput assay utilizing TR-F to detect the properties of binding proteins (e.g., actin-binding proteins, ABPs) using a fluorescence lifetime plate reader and fluorescent probes. In particular, the present invention is a new use of TR-F technique to rapidly evaluate binding of F-actin (− or +Tm) with cMyBP-C in solution. Changes in labeled actin fluorescence lifetime due to cMyBP-C phosphorylation and / or HCM mutations correlated with binding measured by traditional cosedimentation. The present invention features methods for HTS for identifying molecules that modulate cMyBP-C or cMyBP-C-actin complex.

A method of determining platelet aggregation forces is provided. The method involves embedding tension sensor-coated microbeads in platelet clots and observing fluorescent signals from the microbeads that correspond to contractile forces exerted on the tension sensor-coated microbeads to report platelet aggregation forces. In one embodiment, the platelet aggregation forces are used as biomechanical markers to evaluate platelet functions. In another embodiment, the fluorescent signals can be read directly by a plate reader.

A ready-to-use assay kit for modular strip-well plates employs detachable strips whose wells contain co-located, mutually reactive reagents kept apart during storage by an in-well sequestration structure that encapsulates at least one reagent. Upon addition of an aqueous sample, buffer, or water, the structure releases (e.g., rupturable capsules / microcapsules or a phase-change encapsulant), enabling in-well mixing to form a reaction mixture that yields a detection signal. The kit is configured to operate without transfer of reagents between wells prior to reading. Optional features include in situ lyophilization with lyoprotectants, controlled residual moisture, and an inert-gas headspace. Calibrator and control strips may be provided. Detection can be by absorbance, fluorescence, or luminescence using a plate reader.

The present invention discloses a drug sensitivity detection method, including setting a microplate, culturing the microplate, and regularly collecting fluorescence signals and analyzing data. Based on the characteristics of bacterial growth, the present invention utilizes the advantages of fluorescence interpretation to achieve real-time monitoring and analysis, can obtain drug sensitivity results faster, terminate monitoring in time, and has a short time and high efficiency; and has low requirements for equipment, and can achieve monitoring and analysis using a commonly used multifunctional plate reader with a luminescence detection function.

The present invention relates to a method for the specific detection of one or more microorganisms by in-situ hybridization using a microtiter plate reader, a cell drop cell counter or a flow cytometer.

A microplate reader acquires Raman measurements from liquid samples contained in non-adjacent wells in a well plate. At least two Raman probes are positioned perpendicular to the microplate to acquire Raman spectral data of the non-adjacent liquid samples. Each probe includes a lens coupled to a laser and a spectrometer to focus the laser light into the sample and collect light from the sample for the spectrometer. The spectrometer may include a 2D imaging sensor and simultaneously image light from multiple probes that are spaced apart from each other to reduce crosstalk. A positioner moves the microplate or the probes to acquire data from different subsets of the non-adjacent samples and may also change the laser focus within the wells during data acquisition. Multiple fluorescent probes simultaneously acquire fluorescent data from the same sample or non-adjacent samples. The Raman and fluorescent probes are fiber coupled and positioned within the reaction chamber of the liquid handler system.

The present disclosure provides the use of a fluorescent imaging plate reader (FLIPR) high-throughput real-time fluorescence detection and analysis system in detection of calcium signals in plants, which successfully applies the FLIPR to the detection of calcium signals in plants, and increases the use of the FLIPR in the detection of calcium signals in plants. In addition, a method for detecting calcium signals in plants based on FLIPR provided by the present disclosure detects changes in calcium signals generated by stimulation of plants by an exogenous calcium signal CaCl2 solution, which is the basis of high-throughput real-time fluorescence detection of calcium ions in plants.

The invention belongs to the technical field of crossing of nano materials, biosensing and medical detection, and particularly relates to an exosome colorimetric detection method based on MnO2 / CeO2 heterojunction nano enzyme. The MnO2 / CeO2 heterojunction structure is constructed, a stable Mn-O-Ce synergistic interface is formed, electron transfer and interface charge separation are effectively promoted, and the peroxidase-like catalytic ability of the material is improved. The color development reaction is completed through the paper-based platform, an HSV color space analysis system is integrated, and a numeralization result can be obtained by using a smart phone for photographing. Paper-based colorimetric analysis, an intelligent mobile phone auxiliary chromaticity identification system and micro-plate reader quantitative verification are combined. Compared with a traditional RGB method, the HSV model can more accurately reflect hue and brightness changes, the recognition stability and the anti-jamming capability are improved, and visual preliminary screening of high-risk samples in a non-professional environment is achieved.

A microplate reader simultaneously obtains Raman measurements from samples contained in non-adjacent wells. At least two Raman probes are positioned perpendicularly above or below the microplate to simultaneously acquire Raman spectra data of the non-adjacent liquid samples. Each probe is coupled to a laser and a spectrometer and includes a lens focusing laser light within the sample and collecting light from the sample for the spectrometer. The spectrometer may include a 2D imaging sensor (sCMOS or CCD) to image light from multiple probes simultaneously, spaced from one another to reduce crosstalk. A positioner moves the microplate plate or probes to acquire data from a different subset of non-adjacent samples, and may also vary laser focus within wells during data acquisition. Multiple fluorescence probes may simultaneously acquire fluorescence data from the same samples, or non-adjacent samples. Probes may be fiber-coupled and positioned within a reaction chamber of a liquid handling system.

The present invention generally relates to a solid-state, multi-well plate reader including an emitter assembly having a plurality of emitters and a receptor assembly having a plurality of receptors, where the positions of these assemblies are not fixed relative to each other but are temporarily aligned for measurement by way of two alignment trays.

The present invention relates to a method for specifically detecting a microorganism or a plurality of microorganisms by in-situ hybridization using a microtiter plate reader, a cell drop cell counter or a flow cytometer.

CRISPR diagnostic systems have shown great potential for detecting SARS-CoV-2, but improvements in sensitivity, specificity, and turnaround time are needed. Typically, a gRNA with CRISPR-Cas12a is used to detect the target DNA and activate its collateral activity, which is then used to cleave a reporter probe. Here, we demonstrate that the parallel use of two or three gRNAs accelerates the probe cleavage rate by up to 4.5 times compared to individual gRNAs. This synergistic effect is related to the parallel activation of CRISPR-Cas12a and the individual activity of the gRNAs. This observation led to a system that, using two gRNAs, detected the SARS-CoV-2 RNA gene N at up to 10 viral copies after prior amplification with RT-LAMP.The proof of concept of the system was demonstrated with 100% detection of positive and negative clinical samples in ~25 minutes using the fluorescence plate reader and ~45 minutes for lateral flow test strips.

The present invention refers to a method for high-throughput screening of viability of cells, the method comprising the steps of: a) treating, in a well of a first multi-well plate, a sample containing cells with a condition that modulates lifespan; b) transferring a portion of the cells in the well of the first multi-well plate into a well of a second multi-well plate; c) mixing, in a well of the second multi-well plate, the portion of cells with a fluorescent viability dye to stain the cells; d) measuring fluorescence intensity of the stained cells in a plate reader to obtain a fluorescence intensity value; e) measuring optical density of the cells in the plate reader to obtain an optical density value; and f) normalizing the fluorescence intensity value with the optical density value, wherein the normalized fluorescence intensity value directly correlates with the viability of the cells.