40 results about "Calcium signaling" patented technology

The invention discloses an electrical stimulation type nerve regeneration method based on calcium signal regulation and control, and relates to the technical field of electrical stimulation type nerve regeneration, and the method comprises the following steps: based on a Schwann cell population calcium signal response feature set, identifying whether the calcium ion concentration is in a microliter stage which breaks through a lowest activation threshold and does not reach a stable state; judging whether the target nervous tissue is in a calcium signal activation critical state or not based on the behavior mode change of the Schwann cell population; based on the judgment result of the calcium signal activation critical state, an electrical stimulation adjustment control instruction is generated in real time in combination with the calcium ion concentration fluctuation trend and Schwann cell population response characteristics, and the calcium ion concentration stability is optimized and the activation state is maintained by adjusting the electrical stimulation intensity, the electrical stimulation pulse width, the electrical stimulation waveform type and the electrical stimulation application time sequence. According to the method, the problem that a calcium signal is difficult to recognize and dynamically optimize in a microliter stage is solved, and precise maintenance of Schwann cell activation and improvement of nerve regeneration efficiency are realized.

The present invention provides an antibody binding a human tumor-associated calcium signal sensor 2 (Trop-2) protein or fragments thereof, and use of the antibody or fragments thereof in preventing or treating diseases. The antibody or fragments thereof of the present invention can effectively bind to the human Trop-2 protein, and have internalization activity, and the internalization activity is enhanced after ADC drug labeling, and the in vivo efficacy and safety of a mouse model are not lower than those of a control antibody.

The invention discloses an XXM-ER mutant for regulating and controlling plant endoplasmic reticulum calcium ion homeostasis, nucleic acid and application, and belongs to the technical field of biological engineering. The XXM-ER mutant has two amino acid mutations of H134Q and D156H on a light-operated cation channel protein ChR2, and the amino acid sequence of the mutant is as shown in SEQ ID NO. 1. The XXM-ER mutant disclosed by the invention has better endoplasmic reticulum localization, can realize precise regulation and control of plant calcium ion flow through illumination regulation and control, and has great application value in the aspects of plant cultivation, plant calcium signal research and the like.

The present disclosure relates to an antibody-drug conjugate (ADC) targeting human TROP2 (tumor-associated calcium signal transducer 2, TACSTD2)) and use thereof, and more particularly, to: an ADC including an antibody or antigen-binding fragment thereof that binds to human TROP2; and use of ADCs for producing a drug for the treatment and / or treatment of diseases, more particularly, hyperproliferative and / or angiogenic diseases, such as cancer diseases.

The invention relates to the technical field of preparation of organoids, and discloses a method for preparing retinal organoids by constructing pace-making cells on the basis of double transfection of hESC (human ESC) by ChR2 and GCaMP5G, which comprises the following steps: step 1, transfection: transfecting ChR2 and GCaMP5G in the hESC; step 2, screening: screening double positive cells of ChR2 and GCaMP5G through FASC (Fibroblast Amplification Specific Cells); step 3, performing differentiation culture: performing differentiation culture on the two-dimensional ChR2 and GCaMP5G double-positive hESC to obtain a three-dimensional retina organ; and 4, photostimulation: activating ChR2 by using 470nm blue light, and observing a calcium signal under a two-photon microscope. According to the scheme, the ChR2 and the GCaMP5G are transfected in the hESC, the double-positive cells of the ChR2 and the GCaMP5G are screened and then cultured into the retina organoid, and the organoid is stimulated by specific blue light, so that the speed of differentiating and culturing the hESC into the three-dimensional retina organoid is effectively increased, the preparation speed of the retina organoid is effectively increased, and the period is shortened.

The invention discloses a non-destructive culture medium replacement device and method for microalgae, and relates to the technical field of microbiological treatment. Comprising a frame body, a water storage assembly used for storing pure water and calcium-free seawater is arranged above the frame body, a replacement box is arranged in the middle of the frame body and used for containing a dialysis bag containing algae liquid, and the pure water or the calcium-free seawater can flow in and out at the same flow speed through a pipeline and a flow control valve; a collecting assembly used for receiving water discharged from the replacement box is arranged below the frame body. And two groups of symmetrically arranged transverse frames are fixedly mounted in the middle of the frame body. According to the device, a traditional centrifugal method is replaced by physical isolation and micro-vibration auxiliary replacement of the dialysis bag, a knocking assembly driven by a cam is matched with a spring buffer structure, the movement of liquid in the bag is accelerated, meanwhile, mechanical impact force is controlled within a certain amplitude range, it is ensured that the structure of a microalgae cell membrane is complete, and the survival rate is greatly increased; and a high-activity sample is provided for subsequent calcium signal research.

The invention relates to the technical field of biology, in particular to a microwave exposure method for reducing excitability of glutamate neurons. The special microwave exposure condition provided by the invention can significantly reduce the co-expression proportion of medial prefrontal lobe glutamate neurons and c-Fos, the calcium activity of glutamate neurons and the activity of glutamate neural circuits, so that the expression and calcium signal level of immediate early protein c-Fos of glutamate neurons are significantly reduced.

Disclosed herein are compositions and methods for improving pancreatitis through pharmacological manipulation of calcium signaling. Such compositions and methods can be used to improve symptoms of acute or chronic pancreatitis or to reduce the likelihood or severity of pancreatitis in individuals at risk for the condition. In other embodiments, disclosed herein are compositions and methods for improving viral disease through pharmacological manipulation of calcium signaling. In further embodiments, disclosed herein are compositions and methods for improving Thl7-induced disease through pharmacological manipulation of calcium signaling.

The invention relates to the technical field of biological detection and toxicological evaluation, and discloses a neural behavior multi-parameter integration-based caenorhabditis elegans exposure evaluation method which comprises the following specific steps: S1, synchronous culture of caenorhabditis elegans; s2, pollutant exposure treatment; s3, multi-parameter behavior detection; s4, data standardization processing; and S5, constructing a comprehensive evaluation model, and performing weighted integration on four core parameters including the movement speed, the chemotactic index, the synaptic spot density and the calcium signal amplitude after standardization to construct a neurotoxicity index NTI. According to the method disclosed by the invention, a multi-layer and multi-dimensional neurotoxicity evaluation system is constructed by integrating four types of neurobehavior parameters including motion behaviors, chemotactic reactions, synaptic morphology and neuron calcium signals, and the defect that a traditional single endpoint index cannot comprehensively reflect overall disturbance of a neural regulation network is overcome; by utilizing the high homology of the nervous system of caenorhabditis elegans and the human nerve gene, the clinical correlation and mechanism analysis capability of environmental pollutant neurotoxicity evaluation are remarkably improved.

This application relates to the field of plant genetic engineering technology, specifically disclosing the application of a sterol synthesis-related gene in the response regulation of plant salt tolerance. This application clarifies the sterol synthesis-related gene. SMT2 This study expands our understanding of the role of sterol biosynthesis in plant stress response by revealing the function of calcium signaling under salt stress; it also unveils a novel mechanism by which sterol-membrane lipid interactions regulate calcium signaling, providing a new perspective for research on plant salt stress signal transduction; furthermore, this application provides new salt tolerance gene resources. SMT2 Genes can be used for molecular breeding of salt-tolerant crops, to breed salt-tolerant crop varieties, or to develop salt-tolerant molecular markers.

The application discloses a kind of astrocyte dynamic calcium event adaptive threshold segmentation method and device, it is related to cell calcium signal identification technical field, including: original video is carried out calcium signal enhancement denoising, constructs time sequence mask dynamic tracking calcium active event, based on Gaussian mixture model, gray distribution is fitted as multiple Gaussian components superposition and segmented estimation extracts active calcium signal, calculate highlight pixel proportion and interframe similarity index, adaptive dynamic update global threshold and local threshold, generate calcium signal image by window function transformation.Can be in high noise, low signal-to-noise ratio astrocyte calcium imaging video, effectively suppress random imaging noise and preserve weak signal, significantly improve the transient calcium event detection capability of astrocyte, adaptively identify the brightest active calcium signal peak, dynamically adapt the brightness distribution change of each frame image, stably and accurately segment out dynamic calcium event, robustness is strong, degree of automation is high.

This invention belongs to the field of biomedical technology, specifically relating to an anti-tumor nanomaterial, its preparation method, and its application. The anti-tumor nanomaterial is formed by in-situ growth of calcium peroxide on the surface of black phosphorus nanosheets to create hierarchical black phosphorus-calcium peroxide nanosheets, followed by coating the surface of these composite nanosheets with hyaluronic acid. After entering tumor cells, the anti-tumor nanomaterial releases calcium ions and hydrogen peroxide, triggering an abnormal increase in calcium ions and oxidative stress within the cells, thereby causing mitochondrial dysfunction and inducing tumor cell death. Simultaneously, the released calcium ions combine with phosphate ions produced by the degradation of black phosphorus, forming calcium phosphate mineralization deposits within the tumor tissue, inducing tumor biocalcification. Changes in calcium signaling and the oxidative stress process can also promote the infiltration and activation of immune cells, thereby regulating the tumor immune microenvironment and achieving a synergistic effect of cell killing, biocalcification induction, and immune regulation within tumor tissue.

The invention relates to the technical field of plant molecular biology and genetic engineering, and discloses a salt stress resistant gene and application thereof. The salt stress resistant gene is SICA1, and the nucleotide sequence of the salt stress resistant gene is as shown in SEQ ID No.1. The expression quantity of the gene is related to the salt tolerance of the plant. The embodiment verifies that after normal expression or overexpression of the SICA1 gene, calcium signal transduction is normal, and the salt tolerance of the plant is improved.

The application provides a skeletal muscle organoid and a preparation method thereof, takes muscle stem cells, neural stem cells and fibroblasts as primary cells for organoid culture, restores the microenvironment of skeletal muscle as much as possible, and improves the differentiation degree of the organoid; further, the mold and frame for constructing the organoid are improved, the batch production of the organoid is realized through the array design of the PDMS mold and the polyamide frame; finally, the calcium signal video of the organoid is analyzed by using the Organblink system independently developed by the application, and the accuracy of the function evaluation result of the organoid is improved. The method provided by the application realizes the high bionics and high batch production of the skeletal muscle organoid, has low cost, and greatly promotes the development of the medical research field.

A heart tissue model including a heart tissue with at least one inner cavity or a central chamber, wherein the heart tissue model including at least two different heart tissues selected from left ventricle tissue, right ventricle tissue, atrial tissue, outflow tract tissue, atrioventricular canal tissue, sinoatrial node tissue, and atrioventricular node tissue, wherein the central chamber can be shared by at least two different heart tissues, and wherein the at least two different heart tissues include a calcium signaling connection and / or ability to propagate a tissue contraction-; methods of generating such a tissue model and uses of the tissue model for screening purposes is disclosed.

The invention relates to the technical field of animal model construction, in particular to a construction method and application of a gene editing mouse model for studying calcium library function and low fertility in mature sperms.The model is characterized in that tamoxifen is used for inducing, a Ddx4ERT2 promoter is used for driving Cre recombinase expression, three subtypes of IP3R are specifically knocked out in sperms at the same time, and the mouse model is obtained. The method is used for researching the action and mechanism of the subtype in male fertility. After the model mouse is subjected to two rounds of induced knockout, the fertility is severely damaged, the sperm quantity and movement level are reduced, and the acrosome reaction is abnormal, so that the model is a good model for evaluating the sperm dysfunction, and is expected to be used for screening treatment drugs for improving the sperm dysfunction, especially male sterility diseases caused by advanced acrosome reaction in the future. In addition, through screening optimization of an IP3R agonist and a calcium signal detection platform, it is further confirmed that the mouse model is a good model for studying sperm calcium library mediated calcium signals.

The invention discloses a self-adaptive threshold segmentation method and device for an astrocyte dynamic calcium event, and relates to the technical field of cell calcium signal recognition, and the method comprises the steps: carrying out the calcium signal enhancement and denoising of an original video, constructing a time sequence mask to dynamically track a calcium active event, and carrying out the recognition of the time sequence mask; the method comprises the following steps: fitting gray scale distribution into a plurality of Gaussian component superposition based on a Gaussian mixture model, carrying out segmented estimation to extract an active calcium signal, calculating a highlight pixel proportion and an inter-frame similarity index, adaptively and dynamically updating a global threshold value and a local threshold value, and generating a calcium signal image through window function transformation. The method can effectively inhibit random imaging noise and retain weak signals in an astrocyte calcium imaging video with high noise and low signal-to-noise ratio, significantly improves the transient calcium event detection capability of astrocytes, adaptively identifies the brightest active calcium signal peak, dynamically adapts to the brightness distribution change of each frame of image, and improves the detection accuracy. The dynamic calcium event can be stably and accurately segmented, the robustness is strong, and the automation degree is high.

The present invention is directed to novel modulators of the Ca2+-signaling cascade, acting as potentiators of the P2RY2 purinergic receptor and of the inositol triphosphate receptors, ITPRs. The compounds are useful in the treatment of diseases and conditions in which modulation of Ca2+-signaling plays a role, such as in some forms of spinocerebellar ataxia, and in diseases and conditions benefiting from mucosal hydration, as for example respiratory diseases and conditions, dry eye, xerostomia.

This invention discloses a functional analysis system for skeletal muscle organoids and its applications. The core of the system lies in its use of computer vision technology to automatically analyze motion videos of calcium-ion fluorescently labeled skeletal muscle organoids under stimulation. The system automatically identifies organoid regions of interest (ROIs) through specific image processing algorithms (such as adaptive threshold fusion and contour detection), extracts time-series data of calcium signal intensity, and calculates characteristic parameters such as relative fluorescence change rate and peak frequency, ultimately outputting a quantitative functional assessment report. This assessment scheme is low-cost, high-throughput, objective, and accurate, overcoming the technical bottleneck of existing organoid functional assessments that rely on subjective manual analysis or expensive specialized equipment. It is compatible with various culture systems, greatly promoting the large-scale application of skeletal muscle organoids in drug screening and toxicity testing.

The invention relates to the technical field of plant genetic engineering and molecular breeding, in particular to application of an OsCIPK29 gene in enhancing rice blast resistance of rice. The CIPK protein kinase is an important calcium signal decoding factor in plants and plays a key role in calcium signal transduction, adversity stress response and immune signal regulation. According to the application, the expression level of the OsCIPK29 gene in the rice is regulated and controlled by a molecular biology means, so that the immune response capability of the rice under a pathogenic bacterium infection condition is regulated and controlled, and the resistance of the rice to pathogenic bacteria such as rice blast is further improved. Transgenic rice obtained by transferring the OsCIPK29 gene into initial rice shows excellent broad-spectrum property and persistent disease resistance. And the problems of unstable resistance and easy loss in the existing breeding method are effectively solved.

Methods of detecting the level of inositol trisphosphate receptor (IP3R) free calcium (Ca2+) signaling activity in the cultured cells induced by an agonist of IP3R Ca2+ signaling are described herein. The detection of IP3R Ca2+ signaling allows for diagnosing the risk of a patient or subject for developing an Autism Spectrum Disorder (ASD). Additionally, methods described herein could be used for identifying potential therapeutic anti-ASD agents. The methods for treatment and monitoring of the disease are also outlined.

This invention relates to the field of organoid preparation technology, and discloses a method for preparing retinal organoids by constructing pacemaker cells based on ChR2 and GCaMP5G double transfection of hESCs. The method includes the following steps: Step 1, Transfection: Transfecting hESCs with ChR2 and GCaMP5G; Step 2, Screening: Screening for ChR2 and GCaMP5G double-positive cells using FASC; Step 3, Differentiation Culture: Differentiating and culturing the two-dimensional ChR2 and GCaMP5G double-positive hESCs into three-dimensional retinal organoids; Step 4, Photostimulation: Activating ChR2 with 470nm blue light and observing calcium signals under a two-photon microscope. This method, by transfecting hESCs with ChR2 and GCaMP5G, screening for ChR2 and GCaMP5G double-positive cells, and culturing them into retinal organoids, effectively accelerates the differentiation and culture of hESCs into three-dimensional retinal organoids by specific blue light stimulation, thereby effectively improving the speed of retinal organoid preparation and shortening the cycle.

The application discloses a back-carrying visual in-vivo calcium imaging device and belongs to the technical field of in-vivo calcium imaging. The device comprises a device main body, a spine holder and fixing screws. The device main body comprises a track pulley system, four universal wheels and a spine holder base. The track pulley system is composed of two mutually perpendicular metal rods, namely a horizontal track and a vertical track. The bottom end of the vertical track is provided with a microscope fixing platform. The spine holder is connected with the spine holder base of the device main body through four fixing screws and is located below and inside the device main body. The mouse back-carrying visual in-vivo calcium imaging device provided by the application can fix the mouse spine and is not easy to move. The device can be combined with the device main body carrying an ultramicroscope. The device is ingenious in design, simple and reasonable in structure, stable in clamping, good in flexibility, convenient to use and strong in compatibility, and can make the calcium signal recording means more abundant.

According to the skeletal muscle organoid and the preparation method thereof, muscle stem cells, neural stem cells and fibroblasts are used as primary cells for culturing the organoid, the microenvironment of skeletal muscles is reduced as much as possible, and the differentiation degree of the organoid is improved; a mold and a frame for constructing the organoid are further improved, and the batch production of the organoid is realized through the PDMS mold and the polyamide frame which are designed in an array manner; and finally, an independently researched and developed Organblink system is adopted to analyze the calcium signal video of the organoid, so that the accuracy of the function evaluation result of the organoid is improved. According to the method provided by the invention, high bionic property and high-batch production of skeletal muscle organs are realized, the cost is low, and the development of the field of medicine research is greatly promoted.

The present disclosure provides the use of a fluorescent imaging plate reader (FLIPR) high-throughput real-time fluorescence detection and analysis system in detection of calcium signals in plants, which successfully applies the FLIPR to the detection of calcium signals in plants, and increases the use of the FLIPR in the detection of calcium signals in plants. In addition, a method for detecting calcium signals in plants based on FLIPR provided by the present disclosure detects changes in calcium signals generated by stimulation of plants by an exogenous calcium signal CaCl2 solution, which is the basis of high-throughput real-time fluorescence detection of calcium ions in plants.

The present invention provides an advanced coolant composition comprising a volatile agent and a moisturizing lubricant, where the volatile agent comprises ethanol and water, and a non-volatile solvent comprises a diol, glycerol, hyaluronic acid, menthol, and sterol compounds, and is free of any pharmaceutically active ingredient for treating erectile dysfunction. Hyaluronic acid and a sterol compound are self-assembled to form a nanoscale lamellar liquid crystal structure, so that the skin barrier function is kept complete while the transdermal efficiency is improved, and an instantaneous calcium signal activated by a menthol substance TRPM8 receptor and eNOS continuous activation mediated by hyaluronic acid-CD44 generate a remarkable synergistic effect; the dynamic release gradient constructed by the volatile / non-volatile solvent realizes ideal zero-order release kinetics, has excellent effects in the aspects of treatment effect, duration, safety and the like, and provides a brand new non-drug solution for ED treatment.

The invention belongs to the technical field of bioengineering, and particularly relates to an A2aR-His6-G alpha 16-HEK cell strain capable of stably expressing a human adenosine A2a receptor (A2aR). According to the invention, the Galpha16 protein and the A2aR which are determined by screening are sequentially expressed in HEK-FlpIn cells, and the A2aR-His6-Galpha16-HEK cell strain capable of stably expressing the human A2aR is obtained by screening; a cell evaluation model suitable for calcium signal detection is constructed, and the cell evaluation model can be used for screening and detecting A2aR-targeting agonists and antagonists; the kit has the characteristics of more reliable detection result, higher sensitivity, larger screening flux, short experimental period, simplicity and convenience in operation and the like, can greatly improve the screening and evaluation efficiency of A2aR-targeting agonists and antagonists, and meanwhile, has a significant meaning for A2aR-related diseases. Candidate drug molecules are provided for treatment of diseases such as motion control, neurodegenerative diseases, tumor immunoregulation, anti-depression effect, heart diseases, nerve regeneration and immunoregulation, the problem that at present, A2aR target-based drug screening does not have a high-throughput detection method based on calcium signal detection is solved, and the method has obvious practical application value.

Provided herein are compositions and methods related to the treatment of acute pancreatitis (AP) in subjects having accompanying Systemic Inflammatory Response Syndrome (SIRS). The present disclosure provides a method for treating AP including administering a therapeutically effective amount of an intracellular calcium signaling inhibitor to a subject, wherein said subject optionally has accompanying systemic inflammatory response syndrome (SIRS).

Compositions and methods related to the treatment of acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) including viral pneumonia through the pharmaceutical manipulation of calcium signaling are disclosed. Such compositions and methods may be used to reduce pro-inflammatory cytokine releases that may lead to ALI and / or ARDS.

The application provides application of a calcium ion indicator GCaMP in detection of a calcium signal of a human myocardial cell line, successfully applies the calcium ion indicator GCaMP to detection of a calcium signal of a myocardial cell, and increases the use of the calcium ion indicator GCaMP in detection of a calcium signal of a myocardial cell. Secondly, the application provides a method for detecting a calcium signal of a myocardial cell based on the calcium ion indicator GCaMP, and detecting agonism and / or inhibition of a myocardial cell PIEZO1 channel by a small molecule compound, and lays a foundation for research on a myocardial cell related calcium channel.