56 results about "Calcium signaling" patented technology

The invention discloses an electrical stimulation type nerve regeneration method based on calcium signal regulation and control, and relates to the technical field of electrical stimulation type nerve regeneration, and the method comprises the following steps: based on a Schwann cell population calcium signal response feature set, identifying whether the calcium ion concentration is in a microliter stage which breaks through a lowest activation threshold and does not reach a stable state; judging whether the target nervous tissue is in a calcium signal activation critical state or not based on the behavior mode change of the Schwann cell population; based on the judgment result of the calcium signal activation critical state, an electrical stimulation adjustment control instruction is generated in real time in combination with the calcium ion concentration fluctuation trend and Schwann cell population response characteristics, and the calcium ion concentration stability is optimized and the activation state is maintained by adjusting the electrical stimulation intensity, the electrical stimulation pulse width, the electrical stimulation waveform type and the electrical stimulation application time sequence. According to the method, the problem that a calcium signal is difficult to recognize and dynamically optimize in a microliter stage is solved, and precise maintenance of Schwann cell activation and improvement of nerve regeneration efficiency are realized.

The invention provides a calcium imaging neural signal extraction method and product based on self-supervised pre-training, and relates to the technical field of artificial intelligence. Based on a pre-training data set containing a large amount of unlabeled two-photon calcium fluorescence imaging data, a pre-training decoding network and a corresponding pre-training task are self-supervised, and a pre-trained space-time coding network is trained by taking minimization of a pre-training loss function as a target; the method comprises the following steps of: firstly, establishing an encoder and decoder network, establishing fine-tuning data with accurate space-time marked neuron calcium signal positions through simulation, simultaneously performing fine-tuning on the encoder and decoder network by taking minimization of a fine-tuning loss function as a target, and checking the algorithm extraction precision based on various actual imaging data to obtain a calcium imaging signal extraction algorithm network with strong generalization performance. Therefore, a calcium signal extraction model which can be generalized in various imaging conditions and model biological brain regions can be obtained through training without manual labeling, a high-accuracy neural signal extraction result is obtained, the application difficulty that a deep learning model lacks data generalization ability is overcome, and the accuracy of a neural signal extraction result is improved. Calcium imaging neural activity analysis based on self-supervised pre-training and wide in application is achieved.

The present disclosure provides a method of evaluating a mechanism of bipolar disorder, in particular mood switch, in human-derived neurons which may include providing a mechanism of an attenuated protein interaction of CaV1.2 and BK that can cause the calcium signaling deficit, the main cellular phenotype. The present disclosure further provides a method of evaluating a composition for efficacy in treating bipolar disorder, including providing a genetically modified mouse exhibiting BK channel haploinsufficiency; administering the composition to the genetically modified non-human animal; exposing the genetically modified mouse animal to a mild stressor; and determining whether the genetically modified mouse animal experiences a behavioral switch. The present disclosure also provides a method of treating bipolar disorder in a subject in need thereof, including administering to the subject a therapeutically effective amount of a BK channel agonist.

The present invention provides an antibody binding a human tumor-associated calcium signal sensor 2 (Trop-2) protein or fragments thereof, and use of the antibody or fragments thereof in preventing or treating diseases. The antibody or fragments thereof of the present invention can effectively bind to the human Trop-2 protein, and have internalization activity, and the internalization activity is enhanced after ADC drug labeling, and the in vivo efficacy and safety of a mouse model are not lower than those of a control antibody.

The invention discloses an XXM-ER mutant for regulating and controlling plant endoplasmic reticulum calcium ion homeostasis, nucleic acid and application, and belongs to the technical field of biological engineering. The XXM-ER mutant has two amino acid mutations of H134Q and D156H on a light-operated cation channel protein ChR2, and the amino acid sequence of the mutant is as shown in SEQ ID NO. 1. The XXM-ER mutant disclosed by the invention has better endoplasmic reticulum localization, can realize precise regulation and control of plant calcium ion flow through illumination regulation and control, and has great application value in the aspects of plant cultivation, plant calcium signal research and the like.

The present disclosure relates to an antibody-drug conjugate (ADC) targeting human TROP2 (tumor-associated calcium signal transducer 2, TACSTD2)) and use thereof, and more particularly, to: an ADC including an antibody or antigen-binding fragment thereof that binds to human TROP2; and use of ADCs for producing a drug for the treatment and / or treatment of diseases, more particularly, hyperproliferative and / or angiogenic diseases, such as cancer diseases.

The invention provides a device and a method for in-vivo comparison of neuron activity changes in a specific brain region in a mouse odor titer conversion process, and belongs to the technical field of animal experiments. According to the device, a calcium signal acquisition device is pre-embedded in a specific brain region of a mouse, a calcium ion indicator is pre-injected, a first region, a transition region and a second region are arranged in a controllable delivery multi-channel smell condition preference device, and different smells are introduced into the first region and the second region during an experiment; motion trails and residence time of mice in the first area and the second area are recorded through the motion trail recording system, calcium signals of specific brain areas of the mice are collected and analyzed through the neuron calcium signal collecting system, and the odor titer and titer change of the mice are judged. Attraction, avoiding or staying behaviors of the mouse are used as behavioral characterization of odor titer and are associated with neuronal calcium signals in a specific brain region, and the association accuracy is improved under the condition that free activity of the mouse in a limited activity region is not affected.

The invention relates to the technical field of preparation of organoids, and discloses a method for preparing retinal organoids by constructing pace-making cells on the basis of double transfection of hESC (human ESC) by ChR2 and GCaMP5G, which comprises the following steps: step 1, transfection: transfecting ChR2 and GCaMP5G in the hESC; step 2, screening: screening double positive cells of ChR2 and GCaMP5G through FASC (Fibroblast Amplification Specific Cells); step 3, performing differentiation culture: performing differentiation culture on the two-dimensional ChR2 and GCaMP5G double-positive hESC to obtain a three-dimensional retina organ; and 4, photostimulation: activating ChR2 by using 470nm blue light, and observing a calcium signal under a two-photon microscope. According to the scheme, the ChR2 and the GCaMP5G are transfected in the hESC, the double-positive cells of the ChR2 and the GCaMP5G are screened and then cultured into the retina organoid, and the organoid is stimulated by specific blue light, so that the speed of differentiating and culturing the hESC into the three-dimensional retina organoid is effectively increased, the preparation speed of the retina organoid is effectively increased, and the period is shortened.

Compositions and methods related to the treatment of acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) including viral pneumonia through the pharmaceutical manipulation of calcium signaling are disclosed. Such compositions and methods may be used to reduce pro-inflammatory cytokine releases that may lead to ALI and / or ARDS.

The present invention provides an antibody or a fragment thereof that binds to human tumor-associated calcium signal transducer 2 (Trop-2) protein, and the use of the antibody or the fragment thereof for preventing or treating diseases. The antibody or the fragment thereof of the present invention can effectively bind to human Trop-2 protein, has internalization activity, and the internalization activity is enhanced after being labeled with an ADC drug, and the in vivo efficacy and safety in a mouse model are not lower than those of a control antibody.

The invention relates to the technical field of medical experiment animal models, in particular to an animal experiment model and method for calcium signal recording, and the model comprises the following steps: introducing a fluorescent marker from a specific area of a selected target body into neurons of the target body, and arranging a microscopic camera fixing seat at the specific area of the target body, and observing the brain activity of the target body by using a microscope camera. According to the invention, intravenous administration and calcium signal recording model animal construction is realized, and subsequent research on drug effects and treatment mechanisms is facilitated by observing the change of calcium signals at the moment of drug intravenous blood vessel infusion.

The invention discloses a non-destructive culture medium replacement device and method for microalgae, and relates to the technical field of microbiological treatment. Comprising a frame body, a water storage assembly used for storing pure water and calcium-free seawater is arranged above the frame body, a replacement box is arranged in the middle of the frame body and used for containing a dialysis bag containing algae liquid, and the pure water or the calcium-free seawater can flow in and out at the same flow speed through a pipeline and a flow control valve; a collecting assembly used for receiving water discharged from the replacement box is arranged below the frame body. And two groups of symmetrically arranged transverse frames are fixedly mounted in the middle of the frame body. According to the device, a traditional centrifugal method is replaced by physical isolation and micro-vibration auxiliary replacement of the dialysis bag, a knocking assembly driven by a cam is matched with a spring buffer structure, the movement of liquid in the bag is accelerated, meanwhile, mechanical impact force is controlled within a certain amplitude range, it is ensured that the structure of a microalgae cell membrane is complete, and the survival rate is greatly increased; and a high-activity sample is provided for subsequent calcium signal research.

The invention relates to the technical field of biology, in particular to a microwave exposure method for reducing excitability of glutamate neurons. The special microwave exposure condition provided by the invention can significantly reduce the co-expression proportion of medial prefrontal lobe glutamate neurons and c-Fos, the calcium activity of glutamate neurons and the activity of glutamate neural circuits, so that the expression and calcium signal level of immediate early protein c-Fos of glutamate neurons are significantly reduced.

Disclosed herein are compositions and methods for improving pancreatitis through pharmacological manipulation of calcium signaling. Such compositions and methods can be used to improve symptoms of acute or chronic pancreatitis or to reduce the likelihood or severity of pancreatitis in individuals at risk for the condition. In other embodiments, disclosed herein are compositions and methods for improving viral disease through pharmacological manipulation of calcium signaling. In further embodiments, disclosed herein are compositions and methods for improving Thl7-induced disease through pharmacological manipulation of calcium signaling.

The invention relates to the technical field of biological detection and toxicological evaluation, and discloses a neural behavior multi-parameter integration-based caenorhabditis elegans exposure evaluation method which comprises the following specific steps: S1, synchronous culture of caenorhabditis elegans; s2, pollutant exposure treatment; s3, multi-parameter behavior detection; s4, data standardization processing; and S5, constructing a comprehensive evaluation model, and performing weighted integration on four core parameters including the movement speed, the chemotactic index, the synaptic spot density and the calcium signal amplitude after standardization to construct a neurotoxicity index NTI. According to the method disclosed by the invention, a multi-layer and multi-dimensional neurotoxicity evaluation system is constructed by integrating four types of neurobehavior parameters including motion behaviors, chemotactic reactions, synaptic morphology and neuron calcium signals, and the defect that a traditional single endpoint index cannot comprehensively reflect overall disturbance of a neural regulation network is overcome; by utilizing the high homology of the nervous system of caenorhabditis elegans and the human nerve gene, the clinical correlation and mechanism analysis capability of environmental pollutant neurotoxicity evaluation are remarkably improved.

This application relates to the field of plant genetic engineering technology, specifically disclosing the application of a sterol synthesis-related gene in the response regulation of plant salt tolerance. This application clarifies the sterol synthesis-related gene. SMT2 This study expands our understanding of the role of sterol biosynthesis in plant stress response by revealing the function of calcium signaling under salt stress; it also unveils a novel mechanism by which sterol-membrane lipid interactions regulate calcium signaling, providing a new perspective for research on plant salt stress signal transduction; furthermore, this application provides new salt tolerance gene resources. SMT2 Genes can be used for molecular breeding of salt-tolerant crops, to breed salt-tolerant crop varieties, or to develop salt-tolerant molecular markers.

The invention discloses a zebra fish heart electrophysiology detection method and application thereof, and belongs to the technical field of biological tissue dielectric property analysis. The method is mainly carried out by using a Taiwan solution especially suitable for keeping the vitality of the zebra fish heart and a dye solution special for the isolated heart of the zebra fish. The Taiwan solution is an isotonic ion solution, and various ions dissolved in the Taiwan solution can ensure ion exchange of the zebra fish heart, so that the zebra fish heart is prevented from being inactivated due to over-infiltration or water loss conditions and the like. The dye liquor prepared on the basis of the Taiwan's solution can ensure the activity of the zebra fish heart in the long-time dyeing process. The dye liquor is a combination of low-concentration and high-concentration calcium-sensitive infection solutions, and the use sequence is that the high concentration is performed firstly and then the low concentration is performed. The method can meet the requirements of various data required in electrophysiology related literatures, especially can reflect the change condition of calcium signals in cells, and also has the advantages of low cost, short experimental period and the like.

The application discloses a kind of astrocyte dynamic calcium event adaptive threshold segmentation method and device, it is related to cell calcium signal identification technical field, including: original video is carried out calcium signal enhancement denoising, constructs time sequence mask dynamic tracking calcium active event, based on Gaussian mixture model, gray distribution is fitted as multiple Gaussian components superposition and segmented estimation extracts active calcium signal, calculate highlight pixel proportion and interframe similarity index, adaptive dynamic update global threshold and local threshold, generate calcium signal image by window function transformation.Can be in high noise, low signal-to-noise ratio astrocyte calcium imaging video, effectively suppress random imaging noise and preserve weak signal, significantly improve the transient calcium event detection capability of astrocyte, adaptively identify the brightest active calcium signal peak, dynamically adapt the brightness distribution change of each frame image, stably and accurately segment out dynamic calcium event, robustness is strong, degree of automation is high.

This invention belongs to the field of biomedical technology, specifically relating to an anti-tumor nanomaterial, its preparation method, and its application. The anti-tumor nanomaterial is formed by in-situ growth of calcium peroxide on the surface of black phosphorus nanosheets to create hierarchical black phosphorus-calcium peroxide nanosheets, followed by coating the surface of these composite nanosheets with hyaluronic acid. After entering tumor cells, the anti-tumor nanomaterial releases calcium ions and hydrogen peroxide, triggering an abnormal increase in calcium ions and oxidative stress within the cells, thereby causing mitochondrial dysfunction and inducing tumor cell death. Simultaneously, the released calcium ions combine with phosphate ions produced by the degradation of black phosphorus, forming calcium phosphate mineralization deposits within the tumor tissue, inducing tumor biocalcification. Changes in calcium signaling and the oxidative stress process can also promote the infiltration and activation of immune cells, thereby regulating the tumor immune microenvironment and achieving a synergistic effect of cell killing, biocalcification induction, and immune regulation within tumor tissue.

To provide pharmaceuticals for improving pancreatitis symptoms in mammals including human.SOLUTION: Disclosed is a use of an inhibitor of intracellular calcium signaling in manufacture of a medicament for ameliorating human acute pancreatitis symptoms, where the symptoms are symptoms of acute pancreatitis, the intracellular calcium signaling inhibitor is N-(5-(6-chloro-2,2-difluorobenzo[d][1,3]dioxol-5-yl)pyrazin-2-yl)-2-fluoro-6-methylbenzamide or a pharmaceutically acceptable salt or a pharmaceutically acceptable solvate thereof.SELECTED DRAWING: Figure 3A

The invention relates to the technical field of plant molecular biology and genetic engineering, and discloses a salt stress resistant gene and application thereof. The salt stress resistant gene is SICA1, and the nucleotide sequence of the salt stress resistant gene is as shown in SEQ ID No.1. The expression quantity of the gene is related to the salt tolerance of the plant. The embodiment verifies that after normal expression or overexpression of the SICA1 gene, calcium signal transduction is normal, and the salt tolerance of the plant is improved.

The application provides a skeletal muscle organoid and a preparation method thereof, takes muscle stem cells, neural stem cells and fibroblasts as primary cells for organoid culture, restores the microenvironment of skeletal muscle as much as possible, and improves the differentiation degree of the organoid; further, the mold and frame for constructing the organoid are improved, the batch production of the organoid is realized through the array design of the PDMS mold and the polyamide frame; finally, the calcium signal video of the organoid is analyzed by using the Organblink system independently developed by the application, and the accuracy of the function evaluation result of the organoid is improved. The method provided by the application realizes the high bionics and high batch production of the skeletal muscle organoid, has low cost, and greatly promotes the development of the medical research field.

Disclosed herein are isolated polypeptides or polypeptide complexes comprising a tumor-associated calcium signal transduction protein 2 (TROP2) binding domain having optimized binding and kinetic properties. In some embodiments, the isolated polypeptide or polypeptide complex further comprises a CD3 binding domain.

The present invention provides an apparatus and method for comparing changes in neuronal activity in specific brain regions of mice during the process of odor valence change in vivo, and belongs to the field of animal experiment technology. The apparatus of the present invention pre-embeds a calcium signal acquisition device in a specific brain region of mice and pre-injects a calcium ion indicator, sets a first region, a transition region, and a second region in a multi-channel odor condition preference device with controllable delivery, and during the experiment, different odors are introduced into the first region and the second region, and the movement trajectory and residence time of the mice in the first region and the second region are recorded by a movement trajectory recording system. The neuronal calcium signal acquisition system acquires and analyzes calcium signals in specific brain regions of mice, judges the valence and valence change of the mice to the odor, and uses the attraction, avoidance, or residence behavior of the mice as a behavioral representation of the odor valence, and associates it with the neuronal calcium signals in specific brain regions, thereby improving the accuracy of the association without affecting the free movement of the mice in a limited activity area.

A heart tissue model including a heart tissue with at least one inner cavity or a central chamber, wherein the heart tissue model including at least two different heart tissues selected from left ventricle tissue, right ventricle tissue, atrial tissue, outflow tract tissue, atrioventricular canal tissue, sinoatrial node tissue, and atrioventricular node tissue, wherein the central chamber can be shared by at least two different heart tissues, and wherein the at least two different heart tissues include a calcium signaling connection and / or ability to propagate a tissue contraction-; methods of generating such a tissue model and uses of the tissue model for screening purposes is disclosed.

The invention relates to the technical field of animal model construction, in particular to a construction method and application of a gene editing mouse model for studying calcium library function and low fertility in mature sperms.The model is characterized in that tamoxifen is used for inducing, a Ddx4ERT2 promoter is used for driving Cre recombinase expression, three subtypes of IP3R are specifically knocked out in sperms at the same time, and the mouse model is obtained. The method is used for researching the action and mechanism of the subtype in male fertility. After the model mouse is subjected to two rounds of induced knockout, the fertility is severely damaged, the sperm quantity and movement level are reduced, and the acrosome reaction is abnormal, so that the model is a good model for evaluating the sperm dysfunction, and is expected to be used for screening treatment drugs for improving the sperm dysfunction, especially male sterility diseases caused by advanced acrosome reaction in the future. In addition, through screening optimization of an IP3R agonist and a calcium signal detection platform, it is further confirmed that the mouse model is a good model for studying sperm calcium library mediated calcium signals.

The invention discloses a self-adaptive threshold segmentation method and device for an astrocyte dynamic calcium event, and relates to the technical field of cell calcium signal recognition, and the method comprises the steps: carrying out the calcium signal enhancement and denoising of an original video, constructing a time sequence mask to dynamically track a calcium active event, and carrying out the recognition of the time sequence mask; the method comprises the following steps: fitting gray scale distribution into a plurality of Gaussian component superposition based on a Gaussian mixture model, carrying out segmented estimation to extract an active calcium signal, calculating a highlight pixel proportion and an inter-frame similarity index, adaptively and dynamically updating a global threshold value and a local threshold value, and generating a calcium signal image through window function transformation. The method can effectively inhibit random imaging noise and retain weak signals in an astrocyte calcium imaging video with high noise and low signal-to-noise ratio, significantly improves the transient calcium event detection capability of astrocytes, adaptively identifies the brightest active calcium signal peak, dynamically adapts to the brightness distribution change of each frame of image, and improves the detection accuracy. The dynamic calcium event can be stably and accurately segmented, the robustness is strong, and the automation degree is high.

The present invention is directed to novel modulators of the Ca2+-signaling cascade, acting as potentiators of the P2RY2 purinergic receptor and of the inositol triphosphate receptors, ITPRs. The compounds are useful in the treatment of diseases and conditions in which modulation of Ca2+-signaling plays a role, such as in some forms of spinocerebellar ataxia, and in diseases and conditions benefiting from mucosal hydration, as for example respiratory diseases and conditions, dry eye, xerostomia.

This invention discloses a functional analysis system for skeletal muscle organoids and its applications. The core of the system lies in its use of computer vision technology to automatically analyze motion videos of calcium-ion fluorescently labeled skeletal muscle organoids under stimulation. The system automatically identifies organoid regions of interest (ROIs) through specific image processing algorithms (such as adaptive threshold fusion and contour detection), extracts time-series data of calcium signal intensity, and calculates characteristic parameters such as relative fluorescence change rate and peak frequency, ultimately outputting a quantitative functional assessment report. This assessment scheme is low-cost, high-throughput, objective, and accurate, overcoming the technical bottleneck of existing organoid functional assessments that rely on subjective manual analysis or expensive specialized equipment. It is compatible with various culture systems, greatly promoting the large-scale application of skeletal muscle organoids in drug screening and toxicity testing.

The invention relates to the technical field of plant genetic engineering and molecular breeding, in particular to application of an OsCIPK29 gene in enhancing rice blast resistance of rice. The CIPK protein kinase is an important calcium signal decoding factor in plants and plays a key role in calcium signal transduction, adversity stress response and immune signal regulation. According to the application, the expression level of the OsCIPK29 gene in the rice is regulated and controlled by a molecular biology means, so that the immune response capability of the rice under a pathogenic bacterium infection condition is regulated and controlled, and the resistance of the rice to pathogenic bacteria such as rice blast is further improved. Transgenic rice obtained by transferring the OsCIPK29 gene into initial rice shows excellent broad-spectrum property and persistent disease resistance. And the problems of unstable resistance and easy loss in the existing breeding method are effectively solved.