41results about How to "Differentiate" patented technology

A method of detecting and isolating cells that produce any secreted protein of interest (POI), comprising: a) constructing a cell line transiently or stably expressing a cell surface capture molecule, which binds the POI, by transfecting the cell line with a nucleic acid that encodes such cell surface capture molecule; b) transfecting said cell simultaneously or subsequently with a second nucleic acid that encodes a POI wherein such POI is secreted; c) detecting the surface-displayed POI by contacting the cells with a detection molecule, which binds the POI; and d) isolating cells based on the detection molecule.

Recombinant cell surface capture proteins and detection molecules that are useful for isolating and detecting cells that produce a secreted heterodimeric protein of interest (POI) that has an immunoglobulin CH3 domain and / or substituted CH3 domain are provided. Recombinant cell surface capture proteins and detection molecules that isolate and detect bispecific antibodies are also provided. The invention also provides recombinant antigen-binding proteins that are capable of recognizing and binding to proteins of interest that contain a CH3 domain and / or a modified CH3 domain, such as a CH3 domain with or without amino acid substitutions at H95 and Y96 (IMGT).

The present invention relates to use of miR-18b for prevention, treatment, or diagnosis of muscle diseases or neuromuscular diseases, and specifically it was confirmed that, in a muscle disease by gene mutations model, gene mutations reduce miR-18b expression, cause dysregulation of miR-18b signaling pathways, and thus induce calcium signaling, cell differentiation inhibition, and apoptosis. Therefore, miR-18b of the present invention may be used as a target factor for diagnosing and treating muscle diseases caused by gene mutations such as ALS and DMD.

The invention discloses a method for judging a hinge producing annulations of a parallel machine tool. The annulations exist on the surface of a workpiece machined by the parallel machine tool, the corresponding relation of the position of annulations on the surface of the workpiece and the hinge producing the annulations when a connecting rod reversely rotates around the hinge axis of a universal hinge relative to a hinge hole, and on the basis and according to the corresponding relation of the position of annulations on the surface of the workpiece and the hinge producing the annulations when the connecting rod reversely rotates around the hinge axis of the universal hinge relative to the hinge hole, the hinge producing the annulations of the parallel machine tool is judged, and the workpiece of the hinge producing the annulations of the parallel machine tool is a one-layer or multiple-layer cylindrical workpiece machined by the parallel machine tool. The method is used for judging the hinge producing the annulations of the parallel machine tool and performing hinge maintenance, gap adjustment or re-design and replacement service and improves the machining accuracy of the parallel machine tool.

The invention discloses a vertical-shaft wind turbine rotary spindle vibration restraining method which is high in wind energy capturing efficiency. The method includes: acquiring vertical inclination angle of a rotary spindle in real time; calculating vibration intensity of two orthogonal directions in a horizontal section of the rotary spindle and optimal rotation speed of a wind turbine generator under a maximum tracking target; determining expected rotation speed; allowing a rotating speed inner ring controller to drive the rotation speed to reach the expected rotation speed fast; and starting a reverse electric braking function of the rotating speed inner ring controller and a electromagnetic braking mechanism of the rotary spindle when vibration intensity of the spindle exceeds a preset safety regulating threshold; wherein fast rotating speed regulating of the rotating speed inner ring controller is achieved by motor acceleration through directly cutting of part of phase winding excitations or motor deceleration through releasing current chopping limits. By the method, current vibration intensity of the spindle can be calculated in real time, spindle vibration can be restrained, and the wind turbine generator is enabled to acquire the highest wind energy capturing efficiency as far as possible while mechanical stability is maintained.