6 results about "Hedgehog signalling" patented technology

Provided are: a method for producing a spinal cord organoid from a pluripotent stem cell in vitro, the method comprising (1) a step for culturing a human pluripotent stem cell in a culture medium that contains a ROCK inhibitor and a Wnt signal activator but does not substantially contain a TGFβ inhibitor or a BMP signal inhibitor, (2) a step for further culturing the cell obtained in step (1) in a culture medium that contains a retinoic acid receptor agonist and a hedgehog signal activator, and (3) a step for further culturing the cell obtained in step (2) in a culture medium that does not substantially contain a TGFβ inhibitor, a BMP signal inhibitor, a retinoic acid receptor agonist, or a sonic hedgehog stimulator to obtain a spinal cord organoid; and a spinal cord organoid obtained by the method. The spinal cord organoid according to the present application comprises at least a portion of the dorsal side of the spinal cord and a ventral region of the spinal cord.

The invention relates to a method of generating endolymph-producing epithelial cells and in vitro use of a hedgehog signalling inhibitor for differentiation of inner ear progenitor cells into endolymph-producing epithelial cells; in vitro endolymph-producing epithelial cells and an inner ear organoid comprising endolymph-producing epithelial cells. Also provided is in vitro use of an inner ear organoid in drug discovery; the endolymph-roducing epithelial cells for use in a method of treating diseases of the inner ear; and a method of testing one of more therapeutic agents using these cells or organoids. Lastly, also provided is a kit and an organoid maturation medium.

Described herein are compounds and pharmaceutical compositions containing such compounds for the modulation of Hedgehog signaling.

The invention belongs to the technical field of biological medicines, and relates to a tetrahydropyridine [4, 3-d] pyrimidine derivative as a Hedgehog signaling pathway inhibitor and a preparation method thereof. The tetrahydropyridine [4, 3-d] pyrimidine derivative serving as a Hedgehog signal channel inhibitor is prepared by creatively adjusting the reaction temperature and the ratio of reactants to a catalyst, the reaction yield is greatly increased, the reaction conditions are mild, and the derivative serving as the Hedgehog signal channel inhibitor has good activity on pancreatic cancer cells and medulloblastoma.

Described herein are methods of promoting remyelination in a subject having a demyelinating disease by administering to the subject a combination of a steroid hormone and a Hedgehog signaling pathway modulator. Also described are methods of administering a combination of pharmaceutical products, wherein the combination of pharmaceutical products is in a composition suitable for nasal administration.