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High-throughput method for judging human sperm quality by detecting calcium signal

A technology of sperm quality and calcium signal, applied in the medical field, can solve problems such as cumbersome steps, inefficiency, and time-consuming

Pending Publication Date: 2021-05-28
NANTONG UNIVERSITY
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  • Description
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Problems solved by technology

However, these methods are cumbersome and time-consuming, and cannot efficiently and high-throughput screen the changes in calcium signals stimulated by physiological factors corresponding to various physiological indicators.

Method used

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  • High-throughput method for judging human sperm quality by detecting calcium signal

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Experimental program
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Effect test

Embodiment 1

[0022] 1. Processing of Human Semen Samples

[0023] The semen samples collected in the morning were liquefied in a water bath at 37°C for 60 min. Gently blow and mix, take 10 µL of semen, and perform sperm motility detection with Weili Computer-Aided Semen Analyzer (CASA), record various parameters of human sperm, and compare "World Health Organization Human Semen Inspection and Processing Laboratory Manual" 5th edition (WHO5 ) semen standard parameters (sperm motility ≥ 40%, forward motility ≥ 32%, sperm concentration ≥ 15%), and the sperm samples with normal semen parameters in the initial screening, the results are shown in Table 1.

[0024] Table 1. Functional parameters of computer-aided semen analyzer (CASA) for analyzing sperm samples

[0025] sperm concentration total vitality forward movement standard ≥ 15*10 6 / mL

≥ 40% ≥ 32% normal sample 1 52.71*10 6 / mL

55.94% 43.71%

[0026] 2. Washing and Purifying Human Sperm

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Abstract

The invention discloses a high-throughput method for judging human sperm quality by detecting a calcium signal, and belongs to the technical field of medicine. On the basis of a population sperm sample physiological environment, [Ca < 2 + >] i change high-throughput microplate reader detection under stimulation of various physiological factors is performed on the same sample, the regulation condition of [Ca < 2 + >] i by each physiological factor on a big data clinical sample size is taken as a research object, and corresponding physiological stimulation in each process of functional regulation and control of sperms before gamete fertilization is selected, and whether each sperm sample is normal is observed in each aspect in the fertilization preparation process or not.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a high-throughput method for judging the quality of human sperm by detecting calcium signals. Background technique [0002] Normal mature sperm is a long tadpole-shaped cell, which is divided into head, neck, middle, main and terminal segments. Since mammalian mature sperm have a highly condensed nucleus, they are considered transcriptionally and translationally silent cells. Therefore, the regulation of sperm physiological functions mainly depends on the regulation of post-translational protein modifications. Based on intracellular Ca 2+ Signaling plays a key role in the regulation of this modification as an important second messenger, Ca 2+ It plays a central role in sperm capacitation, and also plays an extremely critical role in the regulation of a series of sperm physiological functions such as sperm maturation, hyperactivation, acrosome reaction, and sperm-eg...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N1/28G01N1/34G01N1/30
CPCG01N21/6428G01N21/6486G01N1/28G01N1/34G01N1/30G01N2021/6439
Inventor 曾旭辉彭利忠顾亚云张小宁康杭黄荣祖姚兵
Owner NANTONG UNIVERSITY
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