3 results about "Capacitation" patented technology

The application discloses a pig IZUMO2 eukaryotic protein and a preparation method and application of a monoclonal antibody of the pig IZUMO2 eukaryotic protein. The full-length sequence of pig IZUMO2 nucleotide CDS is verified, a research method for removing the transmembrane structure of the pig IZUMO2 nucleotide CDS and only expressing the extramembrane sequence of the pig IZUMO2 nucleotide CDS in eukaryotes is designed, and the IZUMO2 protein is expressed and secreted by CHO-K1 cells. The protein expressed by the method is close to natural protein, has biological activity and is simple to operate. The qPCR and ELISA tests verify that the IZUMO2 protein can reduce the expression of inflammatory factors in RAW264.7 cells induced by LPS, and has a certain anti-inflammatory capacity. It is found through experiments that the IZUMO2 protein can promote the capacitation of boar sperm, and it is proved that the protein can be applied to the preparation of a reagent for promoting the capacitation of boar sperm.

The invention discloses an application of an Lrrc71 gene or protein as a biomarker in male infertility diseases. The invention reveals that Lrrc71 is a key factor for maintaining male fertility for the first time. Experiments prove that Lrrc71 gene knockout causes male mouse sterility, and the specific manifestation is that the epididymis tail sperm concentration is remarkably reduced, the sperm tail swing amplitude is inhibited, the movement ability and the forward movement sperm proportion are reduced, and the energy-obtaining related protein tyrosine phosphorylation level is reduced. Meanwhile, it is proved that the infertility can be prevented or treated by improving the activity or expression quantity of the Lrrc71 protein, it is determined that the Lrrc71 serves as an innovative drug target for treating male infertility, and a brand new strategy is provided for development of small molecule drugs or gene therapy. The invention has important application value in the fields of precise diagnosis and targeted therapy of male infertility.

The present application relates to the field of animal reproductive biotechnology, and discloses a method for producing in vitro fertilized embryos by live ovum collection of snowflake beef cattle, aiming at solving the problems of uneven quality of oocytes, low efficiency of in vitro fertilization and loose connection of each technical link in the prior art. The method comprises the following steps: screening of donor cattle and superovulation treatment by decreasing injection of follicle stimulating hormone and luteinizing hormone; obtaining oocyte complex by transrectal ultrasonic guided puncture; in vitro maturation culture of the obtained oocyte; in vitro fertilization after capacitation treatment of semen by gradient centrifugation; sequential culture of the fertilized eggs to the stage of morula or blastocyst; morphological evaluation and vitrification preservation of the embryos cultured to the set stage. By the combined application of the standardized superovulation scheme, the precise ovum collection technology, the optimized maturation culture condition and the sequential culture strategy of embryos, the efficiency of embryo production can be significantly improved, and the quality of in vitro maturation of oocytes can be improved.