35 results about "Capacitation" patented technology

This invention relates to a method of producing an auto induced biological barrier against spermatozoids and / or other microorganisms including viruses and / or pre cancers or non invasive cancers involving the production of an autoimmune reaction in a host in need of such auto-inducement. The invention is characterized in that it comprises the steps of producing heat shock proteins inside vaginal and cervical mucosal cells and releasing said heat shock proteins into the vaginal cavity thus inducing an autoimmune response through a trigger and / or stimulation of both innate and adaptive systems and, as a consequence, the production of: a contraceptive effect through the elimination of the migration or free movement of individual or populations of spermatozoids and / or an inhibition of the maturation and / or capacitation process of the same and / or a neutralization or destruction of the same; a prophylactic effect through an elimination of the migration or free movement of individual or populations of other microorganisms including viruses and / or a neutralization or destruction of the same; a prophylactic effect against pre cancers; a therapeutic effect against non invasive cancers. The invention also relates to a device for using said method comprising a hollow body (1) provided with a heat source on the inside as well as with a means (13, 27) of removal of said hollow body from the vaginal or rectal cavity and a means (6, 8, 10) capable of exchanging heat originating from the hollow body.

The invention relates to a horizontal-shaft tidal turbine with logarithmic spiral vanes. The horizontal-shaft tidal turbine comprises a spindle, a case, a thrust bearing, a coupling, a generator, a rack, a base and a water guide cone, wherein the lower side of the case is respectively supported by the rack and the base; the horizontal-shaft tidal turbine is characterized by further comprising logarithmic spiral vanes in two-dimensional logarithmic spiral line shape, a cone-like-shaped hub immersing into tide, and a sealing cover; the generator is connected with the spindle and the cone-like-shaped hub by the coupling, the sealing cover is arranged at the junction of the spindle and the cone-like-shaped hub, the water guide cone is arranged at the tail part of the case at the rear side of the cone-like-shaped hub; and the logarithmic spiral vanes rotate at uniform speed at the periphery of the cone-like-shaped hub by forming gradient stretching along the axial direction of the cone-like-shaped hub to form a spatial warping shape to be uniformly distributed via the thrust bearing. The horizontal-shaft tidal turbine can greatly reduce hydraulic friction collision and reduce loss at the entrance head, thus improving the capacitation effect of the water turbine and well solving the difficulty of allowing ocean fish schools to smoothly pass.

The invention discloses a mouse cryopreserved sperm resuscitation liquid compound and a preparation method and application thereof. The compound comprises a basis solution, methyl B cyclodextrin MBCDand polyvinyl alcohol PVA. The preparation method comprises the steps that firstly, methyl B cyclodextrin MBCD and polyvinyl alcohol PVA are dissolved in the basic solution, after sufficient dissolution, the mixture is filtered through a bacterium filter device, and mouse cryopreserved sperm resuscitation liquid is obtained. According to the compound, methyl B cyclodextrin MBCD and polyvinyl alcohol PVA are added to the sperm resuscitation liquid, the sperm capacitation process is promoted, the capacitation of sperms is improved, the number of the sperms with fertilization capacity is increased, the fertilization probability of the sperms and eggs is increased, and the fertilization rate is increased. The compound is beneficial to successful preservation of mouse sperm resources, and reestablishment of mouse strain resources is increased and accelerated.

Belonging to the technical field of livestock breeding, the invention discloses a bovine sperm capacitation solution and an in vitro sperm capacitation method. The bovine sperm capacitation solution mainly consists of the following components: 6.7500mg / mL NaCl, 0.3000mg / mL KCl, 2.5000mg / mL NaHCO3, 0.0620mg / mL NaH2PO4.2H2O, 0.2960mg / mL CaCl2.2H2O, 0.1000mg / mL MgCl2.6H2O, 0.1120mg / mL sodium pyruvate, 0.0186mg / mL EDTA-Na, 0.0100mg / mL phenol red, 6.0000mg / mL BSA, 1.0000mg / mL glucose, 0.4800mg / mL caffeine, 0.0500mg / mL heparin, and 0.025-0.05mg / mL Nisin. The capacitation solution provided by the invention can effectively ensure the sperm motility, sperm plasma membrane integrity rate, acrosomal integrity rate and mitochondrial activity, and maintains the in vitro sperm capacitation effect.

The invention provides a detection kit of phosphorylation of sperm tyrosine. The detection kit comprises the following components: a capacitation buffer liquid, a fluorescent combined object and a fluorescent solid sealing object. The invention also provides a method for detecting the phosphorylation of the sperm tyrosine by using the kit. Compared with the prior art, according to the invention, each link of methodology is optimally designed, which includes the best buffer liquid matching of capacitation and an upstream method, a stable reaction place offering by special tissue carrier sheets, sperm double-labeling technique formulation combined with specificity, and influences to the detection result by non-sperm substances of cast-off cells in a seminal fluid are eliminated, thereby guaranteeing the methodology reliability, detection result specificity and good repeatability. Because an optimized detection scheme is adopted, the operation steps and the operation time are reduced, and the detection efficiency is improved greatly; because the optimized detection scheme is adopted, the reagent structure is simplified, and the detection cost is lowered to a greater degree; and because the operation is simple, ready-to-use available reagents are adopted, and the performance is stable.

The invention provides a method for evaluating the quality of sperm obtaining energy in vitro. The method specifically includes the following steps of A, collecting a fresh liquefied sperm specimen, washing sperm with a phosphate buffer or a BWW culture solution, sufficiently removing residual seminal plasma components, and separating out sperm in a centrifugal mode; B, adding the BWW culture solution to sperm separated out in the step A, obtaining upstream sperm through an upstream method, counting the number of sperm, incubating sperm for 3-4 hours in a CO2 cell culture box with 5% of in-vitro energy obtaining liquid, conducting centrifugal separation on the obtained supernate, and collecting supernate as a to-be-tested sample; C, measuring the activity of neuraminidase in sperm energy obtaining liquid, and finally obtaining the value of neuraminidase activity / sperm number to evaluate the quality of sperm. By detecting the activity of neuraminidase in sperm in-vitro energy obtaining liquid, diagnosis basis and clinical queries are provided for male infertility patients of unknown clinical reasons.

The invention provides a method for detecting neuraminidase influencing sperm functions. The method is a novel laboratory detecting method for assessing quality and functions of a sperm and can provide detection and clinical consultations for male infertility patients without clear clinical reasons for infertility. The method is characterized in that expressions of neuraminidase in the sperm are visually quantized through single sperm fluorescence strength analysis or are visually observed by means of fluorescence imaging under a microscope, and if the neuraminidase is highly expressed in the sperm, capacitation of the sperm is achieved through external capacitation liquid, and neuraminidase activity is detected in a fluorescence method.

The invention belongs to a transgenic field, and relates to a treatment method for preparing sperm of a transgenic mouse. The treatment method for preparing sperm of the transgenic mouse, comprises: incubating mouse epididymal sperm at 37 DEG C in a 5% CO2 incubator for 8-10min, mixing a linear DNA fragment containing a target gene into the sperm, ice-bathing the sperm at 2-5 DEG C for 25-30min, heat-shocking the sperm at 40-45 DEG C for 1-3min, ice-bathing the sperm at 2-5 DEG C for 3-5min, and then incubating the sperm at 37 DEG C in a 5% CO2 incubator for 50-70min for capacitation The method can raise permeability of a sperm membrane, is convenient for introducing a gene fragment into the sperm, and raises transgenic efficiency. The invention solves the problems of low efficiency, bad stability and difficult passage when sperm is used to prepare the transgenic mouse. An effective method for preparing the transgenic mouse is disclosed.

The invention relates to a new method for cloning embryo with bovine sperms, which comprises the steps as follows: (1) a collected bovine ovary is cleaned, and an ovarium mound-oocyte complex is drawn out for in vitro culture to lead the oocyte to be cultured and developed to a second meiosis metaphase; (2) then dyeing and fluorescence localization are carried out on the nucleolus of the oocyte,and the nucleolus is denucleated to obtain the denucleated bovine oocyte; (3) the semen of an excellent breeding bull is collected or the commercial semen of a self-sex controlled excellent breeding bull is selected and stored in a liquid nitrogen container; (4) the semen is unfrozen and capacitation treatment is carried out on the semen; then the semen is arranged in the injecta of the oocyte; (5) two capacitation sperms are injected into the cytoplast of the denucleated bovine oocyte to obtain a sperm reconstructed embryo; (6) the sperm reconstructed embryo is activated and cultured. The invention overcomes the single mode that the donor cell in the traditional nuclear transfer can only be a somatocyte, and is a new method for generating a bovine embryo by using double sperms to replacethe somatocyte as a donor to be injected into the cytoplast of denucleated bovine oocyte.

The invention provides an RSSI-based low-power consumption passive wireless node networking method. According to the invention, networking is carried out by use of self-capacitation passive wireless nodes, and an optimal transmission router and an Internet with smallest communication consumption are established, so stability and uniformity of the network are ensured and an effect of low-power consumption high-quality wireless communication of a wireless network is achieved on a bidirectional communication function.

The present invention relates to a method of predicting animal litter size using a fertility-related protein marker, and more particularly, to the discovery of a sperm marker that is expressed differently depending on animal fertility, a marker composition for predicting litter size, which comprises an antibody that binds specifically to the marker, and a method of predicting animal litter size using the marker composition. Moreover, the present invention relates to a method of predicting animal semen quality and litter size by chlortetracycline staining, and more particularly, to a method of predicting of animal litter size by measuring the motility, motion kinematics or capacitation status of sperm. When the animal sperm-derived protein marker according to the present invention is used, the litter size of individuals can be predicted by analyzing a protein that is expressed differently depending on litter size. When the method of animal semen quality and litter size by chlortetracycline staining is used, the fertility of sperm and the litter size of individuals can be predicted. According to the present invention, superior species having high sperm fertility and high litter size can be selected based on information provided by the method. Thus, the present invention is highly useful for the sustainable production of animals.