A method of obtaining normal mature sperms of eel relates to a method of obtaining normal mature sperms of eel requiring manual adjusting and controlling to obtain normal mature sperms of eel. The invention comprises: performing backgrounding domestication and food calling on the received catadromous eel parent, causing wild eel to adapting manual breeding environment, increasing corporeity of the eel parent through food calling, storing more matter and energy, preparing for entering the seawater, characterized in that, the eel parent is performed with salinity domestication after being bred for 15 days under manual breeding environment, salinity domestication is transferred from low concentration to sea water; water temperature of a culture pond for the eels is controlled between 18 to 25 DEG C, light is shielded in the day and moonlight is seen at night, flowing water stimulation is performed at night, oxygen concentration in the water keeps more than 6 mg/l, in weak alkalinity water, content of ammonia and nitrogen is not more than 0.02 mg/l and 0.05 mg/l, eel males discharge seminal fluid naturally, normal mature sperms are obtained. The invention is used for fully artificial propagation for the eels.