Artificial insemination method for Filicinae

A fern and sperm technology, applied in the field of artificial insemination of plants, can solve the problems of lagging reproduction technology and low seedling rate of ferns.

Active Publication Date: 2020-10-27
HARBIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problem that the current fern breeding technology is relatively lagging and causes the fern seedling rate to be low, the present invention provides a method for artificial insemination of true fern, comprising the following steps:

Method used

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  • Artificial insemination method for Filicinae
  • Artificial insemination method for Filicinae
  • Artificial insemination method for Filicinae

Examples

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Effect test

Embodiment 1

[0030] Embodiment 1, the artificial insemination method of golden dog fern.

[0031] This example takes Cibotium barometz (L.) J.Sm. as an example to describe the method of extracting and artificial insemination of true fern sperm.

[0032] 1. Cultivation of spores: inoculate the spores into the culture medium, and after preservation in the dark, cultivate them under the conditions of light intensity 1800-4000Lux and light time 12h / d until the maturation of the genitals, and the conditions for the dark preservation are 18- Store in the dark at 25°C for 24 hours; the specific method is as follows:

[0033] (1) Selection of medium

[0034] Put the dark brown soil into a sterilization bag, and sterilize with high-pressure steam at 130°C for 40 minutes. Afterwards, let cool at room temperature and set aside.

[0035] (2) Culture of spores

[0036] Obtaining of male gametophytes: take a culture plate with a radius of 25cm×15cm, spread a piece of 5mm sponge on the bottom of it, ...

Embodiment 2

[0050] The artificial insemination method of embodiment 2. pen holder tree.

[0051] This embodiment takes the pen holder tree as an example to describe the method of extracting and artificial insemination of true fern sperm.

[0052] 1. Cultivation of spores: inoculate the spores into the culture medium, and after preservation in the dark, cultivate them under the conditions of light intensity 1800-4000Lux and light time 12h / d until the maturation of the genitals, and the conditions for the dark preservation are 18- Store in the dark at 25°C for 24 hours; the specific method is as follows:

[0053] (1) Selection of medium

[0054] Put the dark brown soil into a sterilization bag, and sterilize with high-pressure steam at 130°C for 40 minutes. Afterwards, let cool at room temperature and set aside.

[0055] In the present embodiment, cultivating female gametophyte adopts improved Knop's solid medium for cultivation, and the formula of the medium is 0.2g MgSO 4 ·7H 2 O, 0....

Embodiment 3

[0070] Embodiment 3. The method of artificial insemination of Chinese water leek.

[0071] In this embodiment, the method of extracting and artificial insemination of the true fern sperm is described by taking Chinese water leek as an example.

[0072] The cultivation of spores: inoculate the large and small spores into the respective culture medium, and after storing in the dark, cultivate them under the conditions of light intensity 1800-4000Lux and light time 12h / d until the genitals mature. The conditions for the light-proof storage are 18- Store in the dark at 25°C for 24 hours; the specific method is as follows:

[0073] (1) Selection of medium

[0074] Put the humus soil into a sterilization bag, and sterilize with high-pressure steam at 130°C for 40 minutes. Afterwards, let cool at room temperature and set aside.

[0075] (2) Culture of spores

[0076] Obtaining of male gametophytes: take a culture plate with a radius of 25cm×15cm, spread a piece of 5mm sponge on t...

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Abstract

The invention discloses an artificial insemination method for Filicinae, and belongs to the technical field of plant artificial insemination. In order to solve the problem of low seedling rate of pteridophytes, the invention provides an artificial insemination method of Filicinae, which comprises the following steps: inoculating spores into a culture medium, obtaining female gametophytes and malegametophytes of the Filicinae by using a conventional spore culture method, after a sexual organ is mature, sucking mature sperms and a prepared induction liquid, sequentially dropwise adding the mature sperms and the prepared induction liquid to a mature archegonium of the female gametophyte, and enabling the sperms to enter the archegonium to be combined with ova under the guidance of the induction liquid, so as to complete artificial insemination. According to the sperm taking and artificial directional insemination technology provided by the invention, the fertilization rate of the gametophyte can reach 30%-50% and is 3-5 times that under conventional culture conditions, the phenomenon that a single gametophyte has two embryonic developments often occurs, and the hybridization technology of pteridophytes is simpler, more convenient and easier to implement.

Description

technical field [0001] The invention relates to the technical field of plant artificial insemination, in particular to a method for artificial insemination of true ferns. Background technique [0002] There are more than 12,000 species of ferns living on the earth today, and there are about 2,600 species in my country. From ancient times to the present, ferns have played a pivotal role in human life and production. In terms of industry, the spores of Lycopodium lycoides are excellent release agents in the metallurgical industry, and can also be used to make rockets, flares, etc.; All have certain medicinal value. For example, Golden Retriever fern has the functions of nourishing the liver and kidney and dispelling rheumatism, Haijinsha can cure urinary tract infection and stones, and Selaginella can cure damp-heat jaundice and edema. The history of edible ferns is quite long: The rhizome of the fern is rich in starch, and the young leaves have a special fragrance and are of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
CPCA01H1/02Y02A40/81
Inventor 刘保东梅红雪张雪纪善博
Owner HARBIN NORMAL UNIVERSITY
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