81 results about "AI - Artificial insemination" patented technology

The invention discloses a paddlefish breeding method, comprising the following steps of: (1) carrying out parent fish breeding and enriching breeding; (2) selecting parent fish; (3) carrying out artificial induced spawning; (4) carrying out artificial insemination; and (5) breeding by means of unsticking. By adopting the technical scheme, the paddlefish breeding method disclosed by the invention has the following advantages that 1. the whole flow of paddlefish from the parent fish breeding to fish fries breeding can be realized; and 2. the paddlefish is bred and bred by the above technology, so that the paddlefish breeding method is large in parent strain fecundity, good in maturity, high in induced spawning rate, and large in fish fry yield.

An improved artificial insemination device for animals is disclosed. The device has an inner catheter tube inserted inside a catheter tube where the front end of the inner catheter tube is closed in arc shape and its periphery is arranged with a plurality of spray release openings with arc-shaped invagination at outer edges and an extension member that communicates with the interior. As such, when the front end of the inner catheter tube enters the uterus along the cervical tract, it will not scratch the walls of cervical tract during movement because the outer edges of the spray release openings are in arc invaginated shape. When the contraction of the uterus sucks out the animal semen inside the inner catheter tube, it works like a straw through the extension members of the spray release openings, and the semen pooled together inside the catheter could be released rapidly and smoothly into different parts of the uterus to fully enhance the efficiency and the success rate of artificial insemination.

The invention relates to an apparatus and a method useful for non-surgical embryo transfer or artificial insemination of mammals. An apparatus for depositing media into the uterus of a mammal includes a conical chamber that has a plurality of perforations and flaps. An exterior spiral formation is configured for traversing or penetrating cervical passageway. A sheath having a frusto-conical rearward end extends axially from an aft end of the conical chamber. A tubular depositing chamber extends axially from the conical chamber to a position beyond a fore end of the conical chamber. Coupled to the rearward end of the depositing chamber is an embryo or semen packaging unit. The tubular depositing chamber has an end that has an aperture to permit the flow of semen out of the depositing chamber and into the uterus. The invention also involves a method employing the apparatus described.

A device for artificial insemination, wherein the device includes a dildo member having an internal channel for receiving a tubing connected to a pumping mechanism and associated pumping arm, wherein fluid may be drawn into the dildo member through the pumping mechanism and discharged through the internal tubing at a distal end.

The invention relates to a sperm washing liquid and a preparation method thereof and belongs to the technical field of human assisted reproduction. The sperm washing liquid is prepared by dissolving heparin sodium dry powder or heparin sodium salt solution in a salt solution containing antibiotics and indicator. Heparin sodium is added into the sperm washing liquid, and by combination with a receptor on the surface of sperm, heparin can activate a signal transduction system coupled with the receptor, so a capacity function of sperm mitochondria and calcium ion internal flow are improved mildly and effectively, sperm activity is improved, and not only can the artificial insemination success rate be improved, but also safety of the assisted reproductive technology is strongly ensured.

The present invention discloses applications of the compatibility of lycopene and sesamol in livestock semen freezing storage agents, and provides a livestock semen freezing storage agent, which can significantly improve the survival rate of livestock sperms, and comprises a freezing base liquid, egg yolk, lycopene and sesamol. The present invention further provides another livestock semen freezing storage agent, which comprises a freezing base liquid, egg yolk, lycopene, sesamol and glycerol. According to the present invention, the compatibility of lycopene and sesamol is firstly used as the anti-freezing agent for the freezing storage of the semen of pigs, sheep and cows, and the obtained livestock semen freezing storage agent can be used for the artificial insemination of the test tube freezing semen of pigs, sheep and cows, and the long-term storage and the after-long-distance-transportation semen deposition of the semen after the super-temperature freezing, and can significantly improve the survival rate, the acrosome integrity and the plasma membrane integrity of the frozen-thawed perms of pigs, sheep and cows,.

The invention discloses an improved insemination catheter for artificial insemination in animals, characterized in which a center orifice disposed at the rear end of the nozzle for connecting the catheter tube is a blind orifice, and a diversion slit is configured between and connecting the front end of center orifice and the front end of nozzle, the diversion slit being opened when semen in the catheter tube is being squeezed and closed when semen in the catheter tube is not being squeezed. As such, the front end of the nozzle would be closed when it is inserted along with the catheter tube into the vaginal orifice of female animal body during artificial insemination such that contaminants from outside the female animal body or lubricant used will not flow into the center orifice of the nozzle, thereby enhancing the safety of artificial insemination. When the animal semen is squeezed in from the rear end of catheter tube, the diversion slit will open under pressure to allow the semen to be released smoothly, rapidly and accurately into the animal uterus, thereby enhancing the efficiency of artificial insemination. Under the circumstance where the diversion slit is closed when not under pressure, semen flown into the animal uterus or amassed around the cervix will not back flow into the center orifice of nozzle or the catheter tube, thereby reducing wasteful outflow of semen and increasing the chance of conception.

The invention discloses an improved catheter for artificial insemination in animals, comprising primarily a simple improvement of the nozzle mounted at the front end of catheter. The nozzle is predefined on its peripheral surface with pores, channels, or indented texts or patterns of different shapes, which can be used to hold reagents, liquid medicine, ointment or lubricant for animals. As such, when the operator inserts the nozzle into the vagina of female animal body, the procedure achieves not only artificial insemination, but also other purposes, such as testing, experiment and treatment, thereby augmenting the usage and enhancing the efficiency of artificial insemination catheter.

The invention provides a hormone kit for scatophagus argus spawning induction and an artificial spawning induction and insemination method for obtaining scatophagus argus fertilized eggs in batches, which belong to the technical field of aquaculture. The hormone kit for scatophagus argus spawning induction comprises gonadotropin releasing element A4, doxolone maleate and human chorionic gonadotropin which are independently subpackaged. Based on the hormone kit, scatophagus argus is subjected to artificial spawning induction by adopting a three-needle method, so that the problems of long effecttime, female scatophagus argus stagnation, high difficulty in artificial spawn extrusion after spawning induction, egg mass and gonad degeneration are effectively solved. The artificial inseminationmethod is adopted, and the problems that natural spawning eggs of scatophagus argus are over-mature or collapse in vivo, the influence of the environment is large, the spawning induction success rate,the fertilization rate and the hatching rate are extremely low, and fertilized eggs are difficult to obtain in batches are effectively solved.

A method for providing semen optimized for use in artificial insemination is described. Methods involve monitoring sperm cell metabolism by assays that produce results in real time, while sperm are still being processed into doses for use in insemination. Processing is modified in response to assay results, to optimize sperm performance.

The invention discloses a method for optimizing fixed timed artificial insemination effects of gilts. The gilts of the appropriate age are continuously fed with 20 mg/head/day of altrenogest for 18 d,the gilts are injected intramuscularly with 10 mL of AIS/head after feeding of altrenogest is stopped for 42 h, 200 [mu]g of GnRH/head is injected intramuscularly after 80 h, the first artificial insemination is performed after 24 h, and then the second artificial insemination is performed after 16 h. In the method, AIS is used to replace PMSG in the existing fixed timed artificial insemination procedure, the ovulation number of the gilts can be significantly increased, the ovarian cyst rate is reduced, and the hormone secretion of the gilts is improved, so that the optimization of the existing fixed timed artificial insemination technology is realized, and thereby the reproductive performance of the gilts subjected to the FTAI procedure is improved.

The invention belongs to the technical field of fish reproduction and discloses a method for reproducing snakehead fishes by using artificial insemination and a hatching barrel. The method comprises the following steps: temporarily rearing selected parent fishes in a cement pond at the water temperature of 25-26 DEG C and performing spawning induction; in the cement pond at the water temperature of 28-29 DEG C, after the effect time, carrying out inspection and ore squeezing once every 2 h, three times in total; temporarily rearing parent fishes after ore squeezing, preventing inflammation with a penicillin agent to keep the survival rate of female parent fishes at 75% or above; taking testes from male fishes, and grinding the testes in a sperm preservation solution in a separated manner with a metal net; and before fertilization, activating sperm in the sperm preservation solution, then pouring ores for fertilization, hatching the ores in the hatching barrel after leaving the ores tostand for more than 2 h, and inflating the hatching barrel; taking out fertilized ores after hatching the ores in the barrel for 2 days, and performing fry culture after yolk sacs are absorbed 1-2 days later. The method significantly the utilization rate of female parent fishes and the survival rate of the female parent fishes after spawning, reduces field occupation and increases the fry hatchingsuccess rate.

The invention relates to an artificial spawning induction method and hatching method for tylorrhynchus heterochaetus. The method includes the following steps: preparation before spawning induction, selection of parents, artificial insemination, egg washing, and artificial hatching. The method makes full use of the reproductive biological characteristics of the tylorrhynchus heterochaetus, solves the technologies of tylorrhynchus heterochaetus reproductive water environmental conditions, selection of seed insects, male and female parent proportion, insemination time and hatching by artificiallysimulating water area environmental conditions of the tylorrhynchus heterochaetus during natural reproduction, artificially assisting spawning induction and innovating and optimizing a hatching method, shortens the spawning time, improves the spawning amount, insemination rate and hatching rate, and realizes the large-scale artificial propagation of the tylorrhynchus heterochaetus, so that the method lays the foundation for protecting tylorrhynchus heterochaetus germplasm resources, and carrying out reproduction and releasing and artificial breeding, and has important significance for intensive development of tylorrhynchus heterochaetus industry in China.

A method for preparing a diluent for room-temperature storage of muscovy duck seminal fluid is disclosed. The diluent includes 0.5 g of glucose, 0.5-0.85 g of D-fructose, 0.064-0.072 g of magnesium chloride hexahydrate, 0.820-0.852 g of sodium acetate, 1.91-1.94 g of sodium glutamate, 0.062-0.068 g of citric acid, 0.164-0.195 g of trihydroxymethyl aminomethane, 0.1 g of penicillin, 0.1 g of streptomycin and 100 mL of double distilled water, and has a pH value of 7.2-7.6. Sterilization in a boiling water bath is performed after filtration with double-layer filter paper, and after cooling, the diluent is sealed and stored at 4 DEG C and kept for later use. The diluent has good liquid protection effect on the muscovy duck seminal fluid, can solve short-period storage problem of the muscovy duck seminal fluid, can improve the utilization rate of excellent male muscovy ducks, and is simple to operate, good in economic benefit and suitable for seminal fluid storage dilution and artificial insemination, in place of normal saline.

Provided are a method for separating mammalian sperm, by which even a large amount of sperm can be separated in a short time and decrease in motility of the separated sperm can be prevented; and an artificial insemination method, and an in vitro fertilization method. The method for separating mammalian sperm includes incubating the sperm in a medium containing a TLR7 ligand, and separating them into sperm floating in the upper layer and sperm sinking in the lower layer of the medium. The artificial insemination and in vitro fertilization methods include artificial insemination or in vitro fertilization for a non-human mammal using sperm of the non-human mammal separated by the method for separating mammalian sperm.

The invention discloses a portable self-service intravaginal artificial insemination device. The device comprises an outer cylinder body; a limiting plate is fixedly mounted at the bottom end of the lower surface of the outer cylinder body; the side surface of the outer cylinder body is connected with an anti-overflow plate in a sliding manner; a first connector is arranged at the upper end of theouter cylinder body; the outer cylinder body is connected with an insemination head through a first connector in a sleeving manner; an injection tube is fixedly installed in the first connector; an inner cylinder body is slidably connected into the outer cylinder body; a second rubber ring is arranged on the side surface of the inner cylinder body; a push plate is slidably connected into the inner cylinder body; a push rod is fixedly installed on the lower surface of the push plate; and a stress plate is fixedly installed at the lower end of the push rod. A lubricating cavity is formed in theouter cylinder body, and lubricating holes are formed in the side surface of the insemination head, so that the device can output a medical lubricating liquid for lubricating insertion in the insertion process of the outer cylinder body, and vagina and uterine cavity tissues are prevented from being damaged.

The invention provides an ecologic environment-friendly pig farm which comprises a live pig rearing region, an auxiliary production region, a life management region, an isolation environment-friendlyregion and a fruit and vegetable planting region; the live pig rearing region comprises an artificial insemination workshop, a barren gestation workshop, a farrowing lactation workshop, a piglet conservation workshop, a growth fattening workshop, a display workshop and a pig delivery table which are sequentially communicated; pig house floors of the live pig rearing region are each provided with aleak plate, the leak plates are provided with mechanical manure scrapers, and a liquid guiding pipe net is arranged below the leak plates; the isolation environment-friendly region comprises a fecesconcentrated collecting pool, a feces tempering pool and a heterotopia fermentation bed workshop which are sequentially communicated; the feces concentrated collecting pool is communicated with the liquid pipe net; the fruit and vegetable planting region comprises a fertilizer supply unit, and the fertilizer supply unit is communicated with the heterotopia fermentation bed workshop of the isolation environment-friendly region; the pig farm achieves rearing waste disposal and resource recycling, and environment contaminations are reduced.

The invention relates to an artificial insemination assisting device. The device comprises an insemination guiding tube and a display screen. A wireless camera is arranged at the front end of the insemination guiding tube, and the information output end of the wireless camera is connected with the information input end of the display screen. According to the device, the wireless camera and the display screen are used for displaying the working environment of the front end of the insemination guiding tube in real time to help doctors in inspection; multiple through grooves are formed in the axial direction of the front end outer wall of the insemination guiding tube, multiple through holes are formed in through groove inner walls, so that blockage due to the fact that the through holes arebonded by uterine lining is prevented when the doctor inject seminal fluid, the inner wall of a limiting sleeve tube is in threaded connection with the outer wall of the insemination guiding tube, theinner wall of the limiting guiding tube and the outer wall of the insemination guiding tube are provided with fitting limiting bosses to help the doctors to control the guiding depth of the insemination guiding tube in the operation process, and damage on the patient is prevented.

The invention belongs to the technical field of medical instruments, and specifically discloses an artificial insemination transplanting device for reproductive medicine. The artificial insemination transplanting device comprises a device shell, a soft sleeve head, a fixed air bag, a camera, a display screen, a control panel and an injector, wherein a sealing cover is fixedly mounted at one side of the device shell; the bottom end of the sealing cover is fixedly connected with a liquid storage chamber; a controller is fixedly mounted at one side of the interior of the device shell; a liquid guide pipe is arranged in the middle of the interior of the device shell; the soft sleeve head is fixedly installed at the top end of the device shell; a plurality of thin holes are arranged on the softsleeve head; a permeable membrane is fixedly installed at the inner side of the soft sleeve head; and the fixed air bag is fixedly installed in the middle of the device shell. The artificial insemination transplanting device for reproductive medicine provided by the invention can automatically lubricate when entering a vagina to reduce discomfort of a fertilized person, can conveniently find a correct insemination position, prevents a seminal fluid from flowing out and improves the success rate of insemination.

The invention discloses a vas deferens for artificial insemination of pigs, comprising a tube body, wherein the tube body is provided with a head part and a tube body, and the top end of the head partis provided with a sperm infusion port communicated with the tube body, and is characterized in that the head part is wrapped with a cellophane film, a lubricating liquid layer is arranged between the cellophane film and the outer surface of the head part, and the thickness of the cellophane film is 0.1 mm- 0.3 mm, and the tube body is provided with a valve structure, wherein the valve structureis composed of two semicircular membranes with the same size, and the semicircular membrane is provided with an arc edge fixedly connected with the inner wall of the tube body. By providing a cellophane film at the head of the vas deferens and then providing a lubricant layer between the cellophane film and the outer surface of the head, the vas deferens is provided with a lubricant. The problem that the pig is easy to be infected due to the operation of the vas deferens in the prior art is avoided. At the same time, the valve structure prevents sperm return and improves the conception rate ofsows. The vas deferens can be used for artificial insemination of pigs.

The invention discloses an artificial insemination device for reproductive medicine, and the device comprises an injector which is internally provided with semen; the catheter is installed at the injection end of the injector, and semen is injected into the uterine cavity of the patient from the injector through the catheter; the blocking mechanism is located between the catheter and the injector, and the blocking mechanism relatively seals the inner wall of the uterine cavity of the patient and the catheter after the catheter is inserted into the uterine cavity of the patient; the first transmission part is located between the injector and the catheter, and the first transmission part stops filling gas into the blocking mechanism of the patient after the blocking mechanism abuts against the interior of the uterine cavity of the patient; the uterine cavity of a patient is sealed relative to the outside while semen is injected into the uterine cavity of the patient, so that the possibility that the semen flows out is reduced, pregnancy of the patient is facilitated, in addition, the semen in the catheter is injected into the uterine cavity of the patient, it is guaranteed that the semen is completely injected into the uterine cavity of the patient, and the pregnancy possibility of the patient is further increased.