51 results about "AI - Artificial insemination" patented technology

The invention relates to the field of distant hybridization breeding, and discloses an artificial breeding method for hybrid species of humpback perches and epinephelus fuscoguttatus, which comprises the following steps: selecting healthy humpback perches as female parent fishes, and screening healthy epinephelus fuscoguttatus as male parent fishes; carrying out intensive cultivation on the parents by means of nutrient enrichment and temperature regulation and control, and promoting synchronous development and maturation of gonads of the parents; after the parents reach the breeding state, eggs of the female parent fishes and seminal fluid of the male parent fishes are collected respectively, fertilized eggs are obtained through artificial insemination, incubation management such as temperature control and continuous oxygenation is conducted on the fertilized eggs, and finally hybrid grouper fries are obtained. The crossbreeding application of the humpback perch as the female parent and the epinephelus fuscoguttatus is realized, and the problems that in the traditional hybridization process, parent gonad development is asynchronous, and filial generations cannot develop are effectively solved; the bred hybrid provides technical guarantee for standardization and precision promotion of grouper crossbreeding, and creates favorable conditions for breeding new hybrid lines with excellent characters of parents.

The utility model provides an artificial insemination tube assembly, which belongs to the technical field of insemination tubes and comprises a storage tube, a push rod and an annular air bag, the push rod and the annular air bag are arranged on the storage tube, a feeding tube is arranged on the side wall of one end of the storage tube, and a seminiferous duct is mounted at one end of the storage tube. According to the insemination device, the annular air bag arranged on the outer side of the seminiferous duct expands while insemination is conducted, it is guaranteed that the device is fixed, meanwhile, the situation that semen flows back after insemination is effectively prevented, and then the probability of pregnancy is improved, meanwhile, two one-way valves are installed on the two sides of the annular air bag, and after the annular air bag completely abuts against the inner wall of a uterine cavity, the two one-way valves are closed. When the device is taken out, the other one-way valve is forced to be opened and closed to supplement air into the annular air bag from the outside in the subsequent process of taking out the device, so that the push plug is convenient to move to the initial position, and the safety of the device is improved. The subsequent addition and use of the seminal fluid are convenient.

The utility model discloses an artificial semen deposition catheter for a sheep body, which is characterized by comprising an auxiliary tube and a suction head, and the suction head is connected with an injector through the auxiliary tube; one end of the auxiliary pipe is provided with a connector, and the other end of the auxiliary pipe is provided with an inserting part; the suction head is in a hollow cone shape, the inner diameter of the large end of the suction head is in interference fit with the outer diameter of the connector, and the inserting part is connected with an injector. The artificial insemination catheter for the sheep body is good in heat preservation performance, the diameter of the insemination end is small, and the artificial insemination catheter can be easily inserted into the cervix of the ewe to improve the conception rate of artificial insemination.

The utility model discloses an artificial insemination transplantation device, and relates to the technical field of medical instruments. The device structurally comprises a dredging cylinder, a transplanting hose and an injector, the center of the dredging cylinder is provided with a round through hole for the front end of the transplanting hose to pass through; the transplanting hose comprises a circular hose; a semi-spherical end is integrally formed at the front end of the hose; a connecting cap matched with the injector in an inserting manner is integrally formed at the rear end of the hose; the outer wall of the head of the front end of the hose is provided with a circle of evenly-distributed liquid inlets and outlets. Through the design of the dredging cylinder, the transplanting hose can be quickly and accurately inserted into the deep part of the vagina, so that the operation time is shortened, and the complexity is reduced; the injector is isolated from the transplanting hose through the ball, so that seminal fluid is prevented from being in direct contact with the inner wall of the injector, and the integrity and quality of the seminal fluid are guaranteed; the design of the ball enables the position of the ball in the hose to be adjusted according to needs, so that the flowing speed and quantity of seminal fluid can be conveniently controlled, and meanwhile, the ball assists in emptying the seminal fluid in the transplanting hose.

The artificial insemination tube assembly comprises a conveying tube and a pusher, the pusher comprises a liquid storage tube and a piston, the piston slides in the liquid storage tube in a sealed mode, a liquid outlet is formed in the end, away from the piston, of the liquid storage tube, and the conveying tube is arranged at the liquid outlet and communicated with the interior of the liquid storage tube through the liquid outlet. The end, away from the liquid storage tube, of the conveying tube is used for stretching into the body of a patient, the end, away from the liquid storage tube, of the conveying tube is provided with an annular air bag used for blocking the cervical orifice of the patient, and an inflation mechanism used for inflating the annular air bag is arranged between the annular air bag and the liquid storage tube. The annular air bag used for blocking the cervix orifice of a patient is arranged at the end of the conveying pipe, so that semen backflow can be avoided, the outer diameter of the annular air bag is close to the outer diameter of the conveying pipe when the annular air bag is not inflated, and therefore extra discomfort cannot be brought to the patient; the device is integrated with the liquid storage pipe, so that the whole device is more compact, the operation is convenient, and the storage is more convenient.

This invention discloses a method for large-scale breeding of all-male salmon and trout, belonging to the field of fish sex regulation breeding technology. The method includes: collecting fertilized eggs from different families of the same species of salmon and trout, artificially incubating them to obtain fry, and inducing sex reversal in the fry by using 17β-estradiol when they begin feeding; subsequently, raising the fry to sexual maturity, selecting individuals with a female phenotype, and then using sdY as a sex identification gene, selecting pseudo-females with genotype XY by quantitative real-time PCR; pairing the sexually mature pseudo-females with genotype XY with normal males with genotype XY, and obtaining super-males with genotype YY through artificial insemination; pairing the sexually mature super-males with females with genotype XX, and producing all-male salmon and trout with genotype XY on a large scale through artificial insemination. This invention overcomes the problem that existing technologies cannot achieve large-scale breeding of all-male salmon and trout.

The invention belongs to the technical field of artificial insemination of bumblebees, and particularly relates to a male bumblebee sperm collecting device. The male bee seminal vesicle extraction platform is arranged on the base, the male bee seminal vesicle extraction platform is used for clamping the male bee seminal vesicle glass sheet, the male bee seminal vesicle glass sheet is used for placing a male bee seminal vesicle, and the male bee seminal vesicle extraction platform drives the male bee seminal vesicle glass sheet to move in the horizontal direction of the base; the insemination needle fixator is vertically arranged on the base in a lifting mode and used for fixing a glass needle, and the glass needle faces the male bee seminal vesicle glass sheet. Through the device, the extraction efficiency can be remarkably improved, so that the sperm exposure time is effectively shortened, and relatively high vitality is kept.

The invention relates to male bee sperms in which the target region having the nucleotide sequence SEQ ID NO:1 on the JHAMT (Gene ID: 724216) gene is silenced by the forward primer having the nucleotide sequence SEQ ID NO:2 by means of CRISPR-Cas9 technology, and to the insemination of young queen bees with these sperms via artificial insemination. With the repetition of the same procedures, two generation queen bees were bred, and mutant bees were produced, silencing the Jhamt gene. By means of silencing this gene, bees resistant to Varroa destructor parasite are obtained.

The present invention encompasses improved catheters comprising a plurality of tubular bodies, as well as methods of using the improved catheters in artificial insemination.

The application relates to an intracellular injection device and belongs to the technical field of artificial insemination apparatuses. The intracellular injection device comprises an oblique sliding table, a first double-degree-of-freedom magnetic driver installed on the oblique sliding table, a second double-degree-of-freedom magnetic driver connected with the top end of the first double-degree-of-freedom magnetic driver, a third double-degree-of-freedom magnetic driver connected with the front end of the second double-degree-of-freedom magnetic driver, and a needle holding assembly or an ovum sucking assembly fixed to the top end of the third double-degree-of-freedom magnetic driver; the first double-degree-of-freedom magnetic driver, the second double-degree-of-freedom magnetic driver and the third double-degree-of-freedom magnetic driver are of the same structure. The intracellular injection device has the advantages of non-contact driving, large stroke, no hysteresis, high response and mature driving technology; the cell and the cell puncture needle can be moved to a prepared position before puncture with the help of a PWM digital controller.

The invention discloses an intrauterine artificial insemination live productivity prediction method, system and device and a medium, and relates to the field of reproductive medicines.The method comprises the steps that target clinical information is obtained, and the target clinical information comprises clinical information of a to-be-predicted intrauterine artificial insemination patient and clinical information of a to-be-predicted intrauterine artificial insemination patient partner; performing re-sampling on the target clinical information by adopting an SMOTE-Tomeklinks algorithm to obtain target re-sampled clinical information; and inputting the target re-sampling clinical information into an intrauterine artificial insemination living yield prediction model constructed based on an XGboost model to obtain a predicted value of the prognosis intrauterine artificial insemination living yield of the intrauterine artificial insemination patient to be predicted. According to the method, real-time prediction of the intrauterine artificial insemination live-birth rate is realized, the prediction efficiency is improved, the prediction accuracy of the intrauterine artificial insemination live-birth rate is also improved, and meanwhile, the problem of unbalanced training data is solved.

An endoscopic module for artificial insemination or embryo transfer includes a mount portion, a catheter portion, a camera portion, and an air injection portion. The mount portion extends for a predetermined length and has first to third holes formed therein. The catheter portion includes a catheter that is inserted along the first hole portion and extends. The camera portion includes a signal line that is inserted along the second hole portion and extends, and a lens provided at the end of the signal line. The air injection portion includes a tube that is inserted along the third hole portion and extends.

The invention discloses a recirculating water breeding system for simulating natural ocean current and a production promoting method, and belongs to the technical field of aquaculture engineering. The invention discloses a circulating water breeding system and a production promoting method for simulating natural ocean current. The system comprises a culture pond body structure, a bionic water flow stimulation subsystem, a circulating water treatment subsystem and an intelligent control subsystem, the method comprises the following steps of: preparing the system in a pond entering stage of parent fish of the Dongquasichthys nobilis, a gonad development promoting stage, an oviposition inducing stage and a postpartum management stage. The problems that in the prior art, a water flow mode is single and fixed, a gonad development stimulation mode is extensive, artificial insemination is relied on, the automation degree is low, and the overall breeding efficiency is low are solved. According to the method, an integrated circulating water culture environment is constructed, and complex tide and ocean current conditions of a natural spawning site of the Dongcao are accurately simulated, so that gonad development synchronization of parent fishes is effectively stimulated, the natural breeding behavior of the parent fishes is triggered, and the spawning amount and the fertilization rate are remarkably increased.

The present application relates to fish breeding technology field, especially to a kind of all-male tetraploid crucian carp breeding method, comprising the following steps: with gynogenesis homologous diploid crucian carp as female parent, and androgenesis homologous diploid crucian carp as male parent, artificial delivery is carried out;Wherein, the gynogenesis homologous diploid crucian carp sex chromosome type is XX, produces non-reduction diploid egg, and the androgenesis homologous diploid crucian carp male individual sex chromosome type is XY or YY, produces non-reduction diploid sperm;Artificial insemination is carried out, and the obtained zygote is hatched, and cultured to sexual maturity, and the surviving offspring is all-male tetraploid crucian carp population.The present application is based on the characteristics that gynogenesis homologous diploid crucian carp and androgenesis homologous diploid crucian carp both produce non-reduction gametes, artificial insemination is carried out, and all-male tetraploid crucian carp can be prepared on a large scale.The all-male tetraploid crucian carp only needs 120 days of age to reach sexual maturity, can stably produce diploid sperm, and the reproductive performance is improved.

The utility model discloses an artificial insemination sampling device for nursing in the reproductive department. The artificial insemination sampling device comprises a sampling needle cylinder and a control mechanism, a sampling tube is arranged at a sample inlet in the left end of the sampling needle cylinder, a sampling needle head is mounted on the outer arc surface of the sampling tube, an external thread ring is arranged at the right end of the sampling needle cylinder, the outer arc surface of the external thread ring is in threaded connection with a sleeve, a sliding rod is slidably connected into the sampling needle cylinder, a rubber plug is arranged at the left end of the sliding rod, and the outer arc surface of the rubber plug is attached to the inner arc surface of the sampling needle cylinder; according to the artificial insemination sampling device for nursing in the reproductive department, power is provided for movement of an artificial insemination sample in a mechanical driving mode, the artificial insemination sampling operation difficulty is reduced, air in the sample is conveniently exhausted, meanwhile, waste of the sample is reduced, and the labor intensity of workers is reduced. The artificial insemination sampling work of nursing personnel in the reproductive department is more convenient.

The invention discloses an artificial insemination transplantation device for reproductive medicine, and belongs to the technical field of reproductive medicine, the artificial insemination transplantation device comprises a main control machine body and a control panel mounted at the upper end of the main control machine body, a transplantation catheter is mounted in the middle of the main control machine body in a penetrating manner, and one end of the transplantation catheter is connected with an external semen injection device; a sperm outlet hole is formed in the end, inserted into the cervix uteri, of the transplantation catheter, a servo motor is installed in an inner cavity of the main control machine body, and the output end of the servo motor is connected with a transmission gear. According to the artificial insemination transplantation device for reproductive medicine, sliding friction is converted into rolling friction by adopting a slight rotating tube inlet method, injury caused by the fact that the catheter is inserted into the cervix uteri is reduced, meanwhile, part of medical lubricant can flow outwards in the insertion process, the lubricant forms a smooth liquid film between the catheter and tissue, and the tissue is prevented from being damaged. And dry friction is converted into wet friction with much higher lubricity, so that the catheter can pass through more smoothly.

The utility model belongs to the technical field of semen deposition guns, and particularly relates to a semen deposition gun with a semen deposition tube fixing structure. A semen deposition gun with a semen deposition tube fixing structure comprises a sleeve, a handle and a gun barrel, a fixing hole is formed in the handle, the sleeve penetrates into the fixing hole of the handle to be fixed, a fixing section and a handheld section are arranged on the outer wall of the handle, the outer diameter of the fixing section is gradually increased, and a clamping ring is arranged on the fixing section; a plurality of handheld discs are arranged at the handheld section; and a hand pusher is arranged at the tail part of the gun barrel.

The utility model relates to the technical field of reproductive medicine, in particular to an artificial insemination transplantation device for reproductive medicine, which comprises an injector, the injector is slidably connected with a first support cylinder, one end of the first support cylinder is fixedly connected with an expansion component, the injector is communicated with a guide component, and a limiting component is arranged on the surface of the first support cylinder; the expansion assembly comprises a second supporting cylinder and a first rotating cylinder, the first rotating cylinder is provided with a rotating ring, the first rotating cylinder is fixedly connected with a second rotating cylinder, the second supporting cylinder is fixedly connected with a guiding ring, a plurality of guiding grooves are formed in the guiding ring, the second rotating cylinder is rotationally connected with a plurality of expansion pieces, and the bottoms of the expansion pieces are fixedly connected with blocking pieces. The second supporting cylinder is hinged to a plurality of arc-shaped opening pieces. According to the female genital tract dilator, through cooperation of the guide ring, the dilation piece and the arc-shaped dilation piece, the dilation degree of the female genital tract dilator can be adjusted according to the actual situation at different positions in the female genital tract, and therefore a doctor can conveniently conduct artificial insemination operation.

The utility model discloses an artificial insemination fixing device in the technical field of artificial insemination, which comprises a bottom plate, a movable plate is movably arranged on the upper side of the bottom plate, a U-shaped frame is fixedly arranged on the outer side of the movable plate on the upper side of the bottom plate, a fixing rod is fixedly arranged on the inner side of the U-shaped frame, an arc-shaped block is fixedly arranged at the lower end of the fixing rod, and an inner groove is formed in the upper side of the bottom plate. Threaded rods are rotatably arranged on the inner sides of the inner grooves, sliding blocks are arranged on two sections of threads, opposite in direction, on the outer sides of the threaded rods in a sleeving mode, supporting rods are rotatably arranged on the upper sides of the two sliding blocks, and the other ends of the supporting rods are connected to the movable plate. By means of the simple installation structure, a sow can be conveniently lifted and fixed, workers can conveniently conduct artificial insemination on the lifted sow while the sow can be rapidly fixed, using is very flexible and convenient, working intensity is reduced, working efficiency is improved, the practical effect is good, and the sow fixing device is worthy of popularization. The method is worthy of market popularization.

The application discloses a kind of east star spot circulating water breeding system and method for promoting production simulating natural ocean current, belong to aquaculture engineering technical field.The application of a kind of east star spot circulating water breeding system and method for promoting production simulating natural ocean current, system includes: breeding pond body structure, bionic water flow stimulation subsystem, circulating water treatment subsystem and intelligent control subsystem;Method includes system preparation and east star spot parent fish enters pool stage, gonad development promotion stage, spawning induction stage and postpartum management stage.The application solves the problems of single fixed water flow mode, extensive gonad development stimulation method, dependence on artificial insemination, low automation degree and low overall breeding efficiency in the prior art of east star spot circulating water breeding.The application constructs an integrated circulating water aquaculture environment, accurately simulates the complex tidal and ocean current conditions of east star spot natural spawning ground, thereby effectively stimulates parent fish gonad development synchronization, triggers its natural reproduction behavior, and significantly improves spawning quantity and fertilization rate.

The utility model relates to an aquatic fry artificial insemination device, aims at solving the current multiple needle tube placement is more disorderly technical problem, including bottom plate, placing plate and needle tube, bottom plate both sides are respectively fixed with mounting panel, is equipped with mounting hole on mounting panel, placing plate is arranged in bottom plate top side, a plurality of needle tubes are arranged on placing plate in proper order, the needle tube is positioned on placing plate through support structure and clamping structure, the support structure includes bottom block A and support ring, bottom block A is fixed in placing plate top side, support ring is fixed in bottom block A top -end, support ring and needle tube embedded support cooperation, clamping structure includes bottom block B and plastic snap ring, bottom block B is fixed in placing plate top side, plastic snap ring is fixed in bottom block B top -end, plastic snap ring and needle tube embedded joint cooperation, the utility model has the advantage that the needle tube is positioned in placing plate conveniently and quickly, is convenient for taking and keeps placing plate stable simultaneously.

The invention relates to male bee sperms in which the target region having the nucleotide sequence SEQ ID NO:1 on the JHAMT (Gene ID: 724216) gene is silenced by the forward primer having the nucleotide sequence SEQ ID NO:2 by means of CRISPR-Cas9 technology, and to the insemination of young queen bees with these sperms via artificial insemination. With the repetition of the same procedures, two generation queen bees were bred, and mutant bees were produced, silencing the Jhamt gene. By means of silencing this gene, bees resistant to Varroa destructor parasite are obtained.

The invention provides a full-automatic artificial seminiferous duct assembling machine which comprises a pipe body conveying mechanism, a pipe body conveying mechanism, a pipe body conveying mechanism, a pipe body conveying mechanism and a pipe body conveying mechanism. The tube socket carrying mechanism is used for carrying the tube socket conveyed by the straight vibration disc to the station of the tube socket fixing mechanism; the inserting and fixing mechanism is used for carrying out bearing, pressing and fixing on the pipe seat and pushing one end of the pipe body to be inserted into an inner hole of the pipe seat so as to complete inserting and assembling between the pipe body and the pipe seat; the automatic rotary glue spraying mechanism is used for driving the pipe body to rotate so as to realize circumferential glue spraying action on the pipe wall at the other end of the pipe body; the inserting and gluing mechanism is used for inserting the inner hole of the pipe head conveyed by the straight vibration disc into the end part of the pipe body to which glue is sprayed so as to complete rotary inserting, gluing and assembling of the pipe body and the pipe head; the pipe body feeding mechanism is used for automatically feeding the pipe bodies in the storage bin to the pipe body conveying mechanism; the automatic degree, the product quality, the production efficiency and the equipment universality of manual seminiferous duct assembly are improved.

An artificial insemination transplantation device for reproductive medicine belongs to the technical field of reproductive medicine and comprises an outer transplantation cylinder, an injector body and a syringe, the injector body is obliquely mounted in the middle of the interior of the outer transplantation cylinder, and one end of the injector body is inserted into the outer transplantation cylinder and communicated with one end of the syringe. When the artificial insemination transplantation device for reproductive medicine is used for injection, the position adjusting device is connected with the middle of the human insemination tube through the traction tube, and the height of one end of the traction tube is adjusted through the arrangement of the slider and the limiting device, so that the position of the front end of the human insemination tube is slightly adjusted; the direction of the human insemination tube can be conveniently adjusted according to the internal condition of the female vagina in the injection process, meanwhile, a doctor can conveniently and smoothly pass the human insemination tube through the female cervix uteri and other parts, damage to genitals is reduced, the doctor can conveniently select the implantation position during sperm injection through adjustment of the front end position of the human insemination tube, and the human insemination tube is attached to the vicinity of an ovum as much as possible. The pregnancy probability is improved.

The invention belongs to the technical field of fish polyploid breeding, and particularly relates to a method for efficiently inducing triploid grass carps with or without intermuscular thorns. The method comprises the following steps: S1, carrying out artificial spawning induction on grass carp parents, and inducing the grass carp parents to oestrus and gamete maturation; s2, collecting sperms and ova of the grass carp, and performing artificial insemination to obtain fertilized ova; s3, placing the fertilized eggs obtained in S2 in a hydrostatic press, applying pressure, continuously treating for 3 minutes, slowly relieving pressure, taking out the fertilized eggs, and placing the fertilized eggs in a hatching environment for continuous hatching to obtain the triploid grass carp. The invention discloses a method for efficiently inducing triploid grass carps with or without intermuscular thorns, which realizes triploid efficient induction of grass carps with or without intermuscular thorns and can realize large-scale construction of high-proportion triploid grass carp groups with or without intermuscular thorns.

The invention relates to the technical field of acrossocheilus fasciatus breeding, in particular to an acrossocheilus fasciatus oviposition inducing device and an oviposition collecting method. Comprising an inducing unit, a collecting unit, a mixing unit and a hatching unit which are sequentially arranged. The induction unit comprises a plurality of induction water tanks which are arranged in parallel, and a sand frame and a lifting gate are arranged in each induction water tank; the sand frames are detachably arranged in the induction water tanks, coarse sand layers are laid at the bottoms in the sand frames, lifting mechanisms are further arranged at the bottoms of the sand frames and used for lifting the sand frames, and the lifting gates of the induction water tanks are independently controlled. The oviposition inducing device is matched with a special oviposition collecting method, so that the problems of frequent trawl in artificial insemination and difficulty in batch obtaining of fertilized oviposition in non-synchronous gonad development of parent fishes can be effectively solved.

The present invention discloses an induction method for efficiently producing triploid male Nibea albiflora, belonging to the technical field of triploid induction of male Nibea albiflora, and specifically relates to nutritional regulation and photothermal regulation of sexually mature pseudo-male Nibea albiflora and female Nibea albiflora, sperm and eggs collection; artificial insemination to obtain fertilized eggs, which are immersed in seawater for treatment and then incubated, and finally triploid male Nibea albiflora is induced and cultivated; when the fertilized eggs are immersed in seawater during artificial insemination and incubation, purine derivatives can be added to the seawater to treat the fertilized eggs, thereby improving the success rate of triploid induction of Nibea albiflora. The method of the present invention has a high success rate of triploid induction of Nibea albiflora, and triploid male Nibea albiflora is successfully obtained under subsequent induction.

The invention belongs to the technical field of alpaca artificial insemination, and particularly relates to a cryopreservation agent for alpaca semen, a preparation method and application. The invention provides a cryopreservation agent for alpaca semen. The cryopreservation agent comprises trihydroxymethyl aminomethane, citric acid, fructose, penicillin sodium, streptomycin sulfate, yolk, glycerin and glucose. Wherein the yolk and the glycerol have a synergistic effect, so that the freezing resuscitation rate of sperms is increased; and citric acid and trihydroxymethyl aminomethane are used as buffering agents to stabilize the pH value of the semen diluent, maintain the activity and function of the semen diluent and reduce the damage of free radicals to sperms. The penicillin sodium and the streptomycin sulfate are used for sterilization. The fructose, the yolk, the glycerol and the glucose have an anti-oxidation effect, the cell structure of sperms is stabilized, the activity of the sperms is maintained, and the citric acid and the saccharides are matched to regulate osmotic pressure so as to protect the sperms from being damaged by freezing. The preparation method of the cryopreservation agent is simple and easy, and the sperm motility of the alpaca semen after cryopreservation can be improved.

The invention discloses a cuora flavomarginata sperm collecting device and an artificial insemination method. The cuora flavomarginata sperm collecting device comprises a box body, a fixing mechanism, a spraying system, a female turtle tail simulation part and a sperm receiving groove. A supporting inclined plate for placing the cuora flavomarginata is arranged in the box body; the fixing mechanism is configured to fix the cuora flavomarginata; the spraying system is configured to spray the turtle back of the cuora flavomarginata; the position of the female turtle tail simulation part corresponds to the position of the tail part of the cuora flavomarginata fixed on the supporting inclined plate; the semen receiving groove is used for collecting semen discharged by the cuora flavomarginata. According to the artificial insemination method for the cuora flavomarginata, artificial insemination of the cuora flavomarginata can be achieved, the artificial insemination technology for the cuora flavomarginata achieves breakthrough from nothing to nothing, the breeding potential of the breeding cuora flavomarginata is brought into full play, and the problems that the cuora flavomarginata is uneven in mating resource distribution, difficult to mate and not fertilized in the artificial feeding environment are fundamentally solved.

1. Name of the product in this design: Packaging box (artificial insemination catheter). 2. Purpose of this design: For product packaging. 3. The key design features of this product are the combination of shape and pattern. 4. The image or photograph that best illustrates the design's key points: a 3D model. 5. The design for which protection is sought includes color.