55 results about "Glass needle" patented technology

Methods for fabricating and manufacturing solid solution perforators (SSPs) using sharp metal or glass needles and/or subsequent molding and use are described. The methods entail making microneedles by various precision machining techniques and micromold structures from curable materials. Various designs of patch, cartridge and applicator are described. Also described are methods for adjusting the microneedle mechanical strength using formulation and/or post-drying processes.

A variable-angle, wide-angle illuminator is disclosed, one embodiment being a small-gauge, variable-angle illumination surgical system comprising: a light source for providing a light beam; an optical cable, optically coupled to the light source for receiving and transmitting the light beam; a handpiece, operably coupled to the optical cable; an optical fiber, operably coupled to the handpiece, wherein the optical fiber is optically coupled to the optical cable to receive and transmit the light beam; an optical assembly, optically coupled to a distal end of the optical fiber, for receiving the light beam and providing the light beam to illuminate a surgical field; and a cannula, operably coupled to the handpiece and optical assembly, for housing and directing the optical assembly to illuminate a selected area, such as a surgical site. The optical assembly can comprise, for example, a fiber/polymer-dispersed-liquid-crystal (“PDLC”) diffuser optically coupled to an optical needle or a nested compound parabolic concentrator (“CPC”) cone. In the PDLC diffuser/needle embodiment, the fiber can be a standard endo-illuminator optical fiber with 0.50 NA or similar value. The light beam from the light source is emitted from the distal end of the optical fiber and provided to the PDLC diffuser for further transmission. The degree of diffusion of the light beam at the PDLC diffuser can be electrically controlled and can be varied from no diffusion to very high degree of diffusion. After passing through the PDLC diffuser, the light beam is provided to a needle or fiber, such as a glass needle or fiber, that transmits the light beam to the surgical site in the eye.

Disclosed is an improved method for separation an purification of rat islet cells, which comprises pouring rat pancreas with novel Liberase RI enzyme, and centrifugalizing rat pancreas cells by employing Ficoll density gradient. The initially purified pancreas cells are cultured over night, and are selected one by one by means of glass needles connected with rubber pipes through the control of the mouth cavity and tongue of the operator.

The invention discloses a method for enhancing the success rate of extracting a transmission electron microscope sample prepared by a focused ion beam. According to the method, in the process of extracting the sample, when the sample is horizontally put at the bottom part of a groove left in sample preparation, a gasket with adjustable angle is cushioned below a chip so as to form a certain angle; the sample is adsorbed on a needlepoint of a glass needle by using the glass needle and is transferred to a sample carrier, therefore, the failure in sample extraction because the sample is horizontally put at the bottom part of the groove left in the sample preparation is avoided; the success rate of the sample extraction is enhanced; the cost is reduced; and the operation is easy and simple.

The invention provides an operating system for egg cell micro-injection. High-speed rotation of a glass needle, which is kept in water, is driven via vibration of a vibrating mechanism, so that an eddy current is caused in the water, subsequently, egg cells are moved and rotated, and finally a purpose of conducting egg cell injection is achieved; in comparison with existing systems that egg cellsare operated via micro-operation manipulators, the operating system provided by the invention can avoid mechanical contact with the egg cells, so that direct or indirect injury to the egg cells is avoided; and the end of the glass needle can conduct continuous rotation on the egg cells in an in-situ mode, so that the egg cells can be easily controlled within the range of a view field and observation can be facilitated; and in comparison with existing systems that the egg systems are operated within micro-fluidic chips, the operating system provided by the invention, which is free from limitation of operating tasks and the size of the egg cells, is strong in operating flexibility.

The invention discloses a production method and application of a needle-point graphene electrochemical electrode. The production method includes the steps of transferring a graphene film synthesized by chemical vapor deposition to the surface of a micro-nano glass needle point, and using liquid conductive silver glue to connect the graphene and a lead to obtain a needle-point graphene electrode; using cobalt nitrate aqueous solution as electrolyte to deposit cobalt hydroxide on the surface of the needle-point graphene electrode by electrochemical deposition, and heating in a muffle furnace at 300-500 DEG C for 2-4 hours to obtain the needle-point graphene electrochemical electrode functionally modified by tricobalt tetroxide. The needle-point graphene electrochemical electrode can be used to detect micro-droplet samples. After surface modification by tricobalt tetroxide, a high-sensitivity electrochemical biosensor available for enzyme-free detection of glucose is obtained. In addition, the needle-point graphene electrochemical electrode can be used to puncture an active cell to detect intracellular metabolic process in real time.

The invention relates to the field of glasses, and discloses a preparation method of a glass needle-shaped defect reflective electron microscope sample. The preparation method comprises the following steps of (1) putting defect-free glass in an erosion liquid to obtain an erosion speed; (2) measuring the minimum distance between a needle-shaped defect and the surface of the defect glass, and obtaining the theoretical erosion time for the erosion liquid to erode the defect glass until the needle-shaped defect is exposed; (3) putting the defect glass into the erosion liquid to erode, controlling the erosion time, and exposing the needle-shaped defect at the surface of the glass. The preparation method has the advantages that the problem of difficult preparation of the needle-shaped defect reflective electron microscope sample is solved; the speed is high, the sample preparation is accurate, and the cost is low.

The present invention discloses a sample preparation method of a nano-material transmission electron microscope in-situ heating chip, and the method comprises the following steps: (1) cleaning a liquid injection device; (2) connecting one end of a Peek tube with one end of a hollow glass needle tube; (3) fixing the hollow glass needle tube to a micromanipulator; (4) drawing a liquid sample with a syringe; (5) removing a liquid suction needle and connecting the other end of the Peek tube with the syringe; (6) putting an in-situ heating chip on an objective table of a microscope; (7) moving the hollow glass needle tube until the position of a tip needle corresponds to a Si3N4 film to be loaded; and (8) injecting, along the Peek tube, a nano material liquid into the hollow glass needle tube by the syringe, and dispersing the nano material liquid through the tip needle onto the Si3N4 film to be loaded. The method can accurately titrate a sample solution, simple preparation is sample, cost can be reduced, comparative experiments on one chip are facilitated, and the usage rate of the chip is improved.

The present invention discloses a method for chicken embryo cultivation and in vivo electroporation of gene. The method comprises the following steps: carrying out shelled culture: a treatment of in situ electroporation of gene is performed for the chicken embryo with the shell after culturing for 2-3 days; carrying out shell removing culture: a fertilized egg is cultured for 3 days, the shell is removed from the fertilized egg, then the fertilized egg without the shell is placed in a culture container, the culture container is placed in a constant temperature and constant humidity incubator to carry out culture; carrying out a treatment step of in situ electroporation of gene: after carrying out shelled culture for 2-3 days, 0.5-1 mul of plasmid (0.5 mug/mul) is injected to spinal cord by using a microinjection capillary glass needle through a stereomicroscope, or after carrying out shell removing culture for 5-6 days, 0.5-1 mul of plasmid (0.5 mug/mul) is injected to an optic tectum position by using the microinjection capillary glass needle through the stereomicroscope, then electrodes are placed on both sides of the spinal cord injected with the plasmid or the optic tectum position injected with the plasmid to carry out the electroporation, wherein the positive electrode is placed on the side having the gene requiring the electroporation; then carrying out tissue sampling, embedding and slicing. According to the present invention, with optimizing the traditional culture method, the shelled chicken embryo culture technology and the shell removing chicken embryo culture technology are established, the survival rate of the chicken embryo culture is improved, and the basis is provided for the chick embryo in vivo in situ electroporation of gene.

The invention discloses a method for enhancing the success rate of extracting a transmission electron microscope sample prepared by a focused ion beam. According to the method, when the sample is randomly adsorbed to the non-needlepoint position of a first glass needle, the first glass needle is taken down and put on a specific metal base; the sample on the first glass needle is adsorbed by using a needlepoint of a second glass needle so that the sample is adsorbed to the needlepoint position of the second glass needle and put on a sample carrier, therefore, the failure in sample extraction because the sample is adsorbed to the non-needlepoint position of the first glass needle is avoided; the success rate of the sample extraction is enhanced; the cost is reduced; and the operation is easy and simple.

The present invention provides a device and method for moving and rotating a microsphere object. A glass needle placed in water is driven by the vibration of a vibration mechanism to rotate at a highspeed to cause eddy current in the water, and finally the purpose of moving and rotating the microsphere object is achieved. Compared with an existing system which operates a microsphere object by a micromanipulator, the device has the advantages that the mechanical contact with the microsphere object is not needed, the direct or indirect damage to the microsphere object is avoided, an end of theglass needle can continuously rotate the microsphere object in an original place, the microsphere object is easily controlled within the field of view, and the observation is convenient. Compared withan existing system for operating a microsphere body in a micro-fluidic chip, the device has the advantages that the device is not limited by an operation task and the size of the microsphere object,and the operational flexibility is high.

The invention provides a method for improving the success rate of a transmission electron microscope's irradiation on a sample. The sample and a damaged carbon film are extracted by the adsorption force of a glass needle in a sampling system, the sample is placed in an intact area on the upper surface of the carbon film, and the transmission electron microscope is employed to photograph the sample again, so that the damage rate of the sample is effectively reduced, the success rate of the transmission electron microscope's irradiation on the sample is improved, and the cost of the process is lowered.

A method for manufacturing an electromagnetic shielding optical window by using electric field-driven jet 3D printing includes as the following steps pretreating hard transparent substrate to reduce surface energy, using a single potential-based electric field to drive the spray deposition 3D printing process and equipment, using a glass needle as a nozzle, using a particle-free nano silver pasteas a printing material, and performing a metal grid structure array on the surface of the pretreated substrate according to a set path print, printing a first layer of metal grid on the substrate, printing the other layers by the self-focusing effect of the electric field driven jet 3D printing until the metal grid structure array is completed; baking or sintering the substrate of the metal grid,and performing sintering according to the set temperature and time, and allowing the non-particle nano silver paste to be converted and reduced into conductive nano silver by post-sintering treatment,completing the conductive treatment, and forming a metal grid structure electromagnetic shielding optical window.

The disclosed plastic-glass disposable injector with drug solution self comprises glass needle barrel, plastic push rod, silica gel seal piston fixed on rod front end with interference fit with bore of glass needle cylinder of scale, drug solution filled in the said room, and needle holder jacketed on the holder. As glass and silica gel has no pollution to solution or modification, this invention can not only be store bottle, but also the injector; it is convenient to pull out the holder cover, assemble the sterilized needle and inject directly, which brings less waste and is practical.

The invention discloses a needle cylinder frame for a hospital, and relates to the field of medical supplies. The needle cylinder frame for the hospital comprises a fixed connecting plate; a lower clamping plate and an upper clamping plate are fixedly connected to the inner side wall of the fixed connecting plate; the upper clamping plate is located over the lower clamping plate; sliding rails arefixedly connected to the opposite inner side walls of the fixed connecting plate, and a liquid collecting box is clamped between the two sets of sliding rails in a matched and sliding mode; a sealingplate is adaptively clamped and fixed to the side wall of the opening of the fixed connecting plate; a fixed cylinder is fixedly connected to the side wall, opposite to the sealing plate, of the liquid collecting box; a telescopic rod is movably arranged on an inner cavity of the fixed cylinder in a sleeving mode; an abutting spring is arranged in the inner cavity of the fixed cylinder; a hole groove is formed in the position, opposite to the telescopic rod, of the sealing plate; and the telescopic rod is adaptively clamped in the hole groove. According to the needle cylinder frame for the hospital, cleanness and tidiness of a placement cabinet or a table top are guaranteed, sealing of a glass needle cylinder located in the fixed connecting plate is achieved, sanitation of the glass needle cylinder is guaranteed, and body health of a patient is guaranteed.

The invention discloses a pretreatment method capable of eliminating polyhydric alcohol matrix interference in tobacco essence analysis. The pretreatment method comprises the steps that 0.5g-1.5g of essence samples and 0.5g-6g of C18 silica gel packing are weighed and placed in a glass mortar, and stirred uniformly by a glass stirring rod; 10g of packing is weighed and subjected to column packing by using 30 mL of methyl alcohol by a wet method, and the process is repeated for three times; a 100/0, 70/30, 50/ 50 and 30/70 methyl alcohol/chloroform system is utilized to elute the samples, and eluents of three later gradients are combined; and an glass needle cylinder type injection syringe with an organic phase filtering membrane is used to absorb and combine the eluents for chromatographic analysis. According to the method, the problem of matrix interference caused by polyhydric alcohol such as ethylene glycol, 1, 4-butanediol, propylene glycol, glycerol and the like is effectively solved, and the effective components of the essence can be accurately, qualitatively and quantitatively analyzed; and moreover, in the pretreatment process, the method has the advantages that the test device is simple and convenient, the effective component loss of the essence is reduced, the step of decompressed concentration is omitted, the matrix interference can be remarkably eliminated, and the like.

The invention belongs to the technical field of micro-injection, and particularly relates to a micro-injection glass needle breaking device and method for insects. A micro-injection glass needle breaking method for the insects includes the following steps of 1) fixing, wherein the tail of a micro-injection glass needle is fixed to a clamping groove, a needle tip is fixed into a tapered hole channel, and a connecting tube is connected to the tail of the glass needle; 2) needle breaking, wherein a sliding cutter is manually moved horizontally to vertically cut off the micro-injection glass needle; 3) needle grinding, wherein a movable cutting block is replaced with a movable grinding block, fine sandpaper is used for further finely grinding the needle tip of the micro-injection glass needlealong the plane of the movable grinding block; and 4) inspection, wherein the stable air pressure value inside the glass needle is detected by a detection device after the glass needle is broken. By means of the micro-injection glass needle breaking device and method, under the assistance of a magnifying glass and a sliding microscopy scaleplate, the opening diameter of the needle tip and the glass wall thickness of the needle breaking portion of the micro-injection glass needle are measured and adjusted in advance, and the stability of the opening diameter of the needle tip and the mechanicalstrength of the needle tip are stabilized.

The invention discloses a lifting device applicable to full-automatic viscosity measurement. The lifting device comprises a piston module, a valve way module and a viscosity tube, wherein the piston module and the valve way module are connected by adopting a connecting hose, a piston mechanism comprises a transmission shaft sleeve, a glass needle cylinder and a piston plunger, the transmission shaft sleeve is connected with the glass needle cylinder, a transmission lead screw passes through the transmission shaft sleeve, the two ends of the transmission lead screw are respectively connected with a piston motor and the piston plunger, a fourth electromagnetic valve, a first electromagnetic valve, a second electromagnetic valve and a third electromagnetic valve are respectively connected with a main measuring tube by virtue of a connecting hose, the third electromagnetic valve is connected with an air valve by virtue of a connecting hose, a fifth electromagnetic valve is connected with an emptying pipe, and the fourth electromagnetic valve is connected with a feeding emptying pipe. The lifting device disclosed by the invention has the advantages that the whole full-automatic viscosity measurement process becomes a full-automatic process, workload of an experimenter is reduced, and safety problems are reduced.

The invention relates to a forming machine for glass cylinder of a pre- potting injector, comprising machine frame, forming mechanism and drive mechanism, characterized in that it in turn comprises glass tube input mechanism, head forming mechanism, tail curling mechanism and annealing mechanism, where there are glass tube conveying mechanisms between them. And it is compact in structure, able to implement automatic operation, and has characters of low manufacturing cost, high production efficiency, and good work environments, and remarkable economic and social benefits.

The invention discloses an extraction and separation method of embryonic stem cells. The extraction and separation method comprises the following steps: S1, the ovary of a mammal is excised, and exogenous hormone is given to make an embryo continue to develop but implantation is delayed; S2, after the embryo develops for 4-6 days, blastocysts are taken by flushing from a uterus for culture, such that trophoblastic cells grow and feeder layer cells are pushed away, and are spread on the bottom wall of a culture dish; and S3, inner cell mass (ICM) cells proliferate, and vertically grow upward toform egg cylindrical structures, the cylindrical structures are picked out by using a fine glass needle under a microscope, and digestive passaging is performed to obtain embryonic stem cell-like colonies. According to the extraction and separation method, a tissue culture method is adopted to extract and separate the embryonic stem cell; by inoculating the blastocysts on a feeder layer, the implantation of blastocysts in vivo is simulated, which is closer to the natural development process; inner cell populations proliferate vigorously, and thus, embryonic stem cell-like colonies are relatively easy to obtain; and the problems that an existing separation method of the embryonic stem cells is difficult, the cells are easy to damage, and the separation of the embryonic stem cells is influenced are solved.