16 results about "Glass needle" patented technology

The invention discloses a method for cultivating triploid kelp sporophytes and identifying ploidy of induced protonema, and belongs to the field of kelp breeding. The method is based on a capillary glass needle separation technology for inducing filamentous somatic cells by kelp and a molecular biological technology for specific marking of sex of kelp gametophytes. The sex specific marker of the kelp gametophyte is used for carrying out PCR (Polymerase Chain Reaction) amplification and 1.5% agarose gel electrophoresis detection on the separated induction protonema single-cell cloning line, so that the genetic sex of the kelp induction protonema can be accurately identified; and the ploidy of the induced protonema of a part of varieties (lines) is directly determined from the molecular level. The method for synchronously detecting the genetic sex and ploidy of the induced protonema breaks through the bottlenecks of chromosome counting and flow cytometry operation, can be completed only through one-time PCR amplification, and is simple, convenient, rapid, short in time consumption, capable of completing detection of a plurality of samples at the same time and low in cost.

The invention belongs to the technical field of artificial insemination of bumblebees, and particularly relates to a male bumblebee sperm collecting device. The male bee seminal vesicle extraction platform is arranged on the base, the male bee seminal vesicle extraction platform is used for clamping the male bee seminal vesicle glass sheet, the male bee seminal vesicle glass sheet is used for placing a male bee seminal vesicle, and the male bee seminal vesicle extraction platform drives the male bee seminal vesicle glass sheet to move in the horizontal direction of the base; the insemination needle fixator is vertically arranged on the base in a lifting mode and used for fixing a glass needle, and the glass needle faces the male bee seminal vesicle glass sheet. Through the device, the extraction efficiency can be remarkably improved, so that the sperm exposure time is effectively shortened, and relatively high vitality is kept.

The utility model belongs to the field of hollow glass, particularly relates to hollow glass with a self-cleaning structure, and aims to solve the problems that when an existing cleaning wiper moves upwards, residual stains are pushed to move upwards, at the moment, the stains are adhered to the inner wall of the top of a frame above the glass, and the cleaning difficulty is increased. A glass main body is fixedly mounted in the frame, and a scraping plate is arranged on the surface of the glass main body; the glass cleaning device further comprises a lifting assembly, the lifting assembly is located in the frame, and the scraping plate can be driven by the lifting assembly to move up and down and scrub the surface of the glass body. According to the self-cleaning device, the self-cleaning function is achieved, the efficient and stable self-cleaning effect is achieved through the design of special materials and a mechanical structure, meanwhile, secondary pollution in the cleaning process is avoided, and the self-cleaning device has high practical value and market prospects.

The invention discloses a prefilled syringe glass needle cylinder flange width cutting device which comprises a base, an X-direction displacement mechanism is installed on the base, a Y-direction displacement mechanism is installed on the X-direction displacement mechanism, a motor support is installed on the Y-direction displacement mechanism, a servo motor, a speed reducer and a transmission shaft are installed on the motor support, and a double-face cam is installed at the tail end of the transmission shaft. The double-face cam comprises a disc-shaped wheel, cam-shaped grooves are formed in the two side faces of the disc-shaped wheel respectively, cam-shaped protrusions with different cam curves are arranged in the cam-shaped grooves respectively, cam curve grooves are formed between the outer contours of the cam-shaped grooves and the outer contours of the cam-shaped protrusions, a left cam curve groove is formed in the left side face of the disc-shaped wheel, and a right cam curve groove is formed in the right side face of the disc-shaped wheel. A right cam curve groove is formed in the right side face of the disc-shaped wheel, and a stroke difference exists between the left cam curve groove and the right cam curve groove. The end face and the two side faces of the glass needle cylinder are machined at the same time through the driving device through the design of the cam curve groove of a special structure and the compact sliding type design.

The utility model discloses a glass needle tube tray for a prefilled syringe, which belongs to the technical field of trays and comprises a tray body and a glass needle tube, reinforcing ribs are arranged in the tray body, a plurality of placing grooves are arranged at the center of the tray body, through holes are arranged above the placing grooves, and the glass needle tube is arranged in the tray body. The containing groove comprises a supporting part at the bottom, limiting parts on the two sides and a groove in the head, the shape of the supporting part is matched with the outline of the glass needle tube, a spiral head is arranged at the top of the glass needle tube, and a handle is arranged at the bottom of the glass needle tube. Through mutual cooperation of the placing groove, the tray body, the supporting part, the limiting part, the groove, the glass needle tube, the first limiting surface, the limiting block, the handle and the spiral head, the glass needle tube can be limited and protected, so that the glass needle tube is prevented from being damaged due to shaking and collision in the transferring process; and therefore, the safety in the transportation process is improved.

The invention discloses a thermodynamic process control method for a glass needle tube for a pre-filled syringe, and particularly relates to the technical field of production of glass for medicine for the pre-filled syringe. Comprising the steps of multi-modal sensing configuration and calibration of a pre-filled glass product, multi-modal sensing fusion and thermodynamic state estimation of a glass needle tube for a pre-filled syringe, online correction and working condition identification of a digital twin model of the pre-filled glass product, and self-adaptive control decision of a thermodynamic process of the pre-filled glass product. Based on an execution and feedback step of a double-stage high-temperature regulation and control process of the pre-encapsulated glass product and a historical data archiving and self-learning optimization step of a thermodynamic process of the pre-encapsulated glass product, multi-source sensing data fusion and linkage of a digital twin model and a reinforcement learning control strategy are utilized; the whole-process refined and intelligent control of the double-stage high-temperature regulation and control process of the pre-encapsulated glass product is realized.

The utility model relates to a simple cell mouth suction pipe which comprises a mouth suction section at the front end, a connecting section at the middle end and a suction / outlet section at the rear end. The mouth suction section comprises a straight pipe and a filter, one end of the straight pipe is connected with one end of the filter, the connecting section comprises a hose, one end of the hose is connected with the other end of the filter, the suction / outlet section comprises a hard connecting pipe, one end of the hard connecting pipe is connected with the other end of the hose, and the other end of the hard connecting pipe is connected with a rubber plug. The center of the rubber plug is provided with an insertion hole into which a glass needle is inserted. The simple cell mouth suction tube provided by the utility model can effectively solve the problem that saliva flows into and gathers in the mouth suction tube, and improves the efficiency and success rate of experimental operation.

The present invention relates to the field of animal oocyte transplantation, and discloses an animal oocyte transplantation needle, which comprises: a transplantation frame provided with an adjustment auxiliary mechanism; the adjusting auxiliary mechanism comprises a vertical support, a top plate, a sliding plate, a vertical plate, an adjusting plate, a universal ball, a connecting rod, a first arc-shaped clamping sleeve, a second arc-shaped clamping sleeve, an injector and a needle body. The vertical support is fixedly installed at the top of the transplanting frame, the bottom of the top plate is fixedly connected with the vertical support, the sliding plate is installed on the top plate in a sliding mode, the adjusting plate is vertically installed on the vertical plate in a sliding mode, and the universal ball is movably installed at the top of the adjusting plate. The device has the following advantages and effects that puncture bleeding and ovary injury can be relieved through the small-diameter beveled glass needle body, the stable supporting structure is matched with the follicle wall difficult to puncture, handheld operation is replaced with precise adjustment and distance setting, the transplantation precision is improved, the process can be simplified, the technical threshold can be lowered, and the core operation problem in oocyte transplantation is effectively solved.

This application belongs to the field of detection instrument technology and discloses a nanoscale glass tip and its preparation method. The nanoscale glass tip includes: a glass body in the shape of a triangular prism, one of the apex of the glass body being the tip angle, the rounded corner diameter of the tip angle being less than 50 nm, and the three adjacent surfaces of the tip angle being covered with an inner metal film and an outer metal film from the inside to the outside. The adhesion of the inner metal film to the glass body and the adhesion of the outer metal film to the inner metal film are both greater than the adhesion of the outer metal film to the glass body; thereby improving detection accuracy and reducing the probability of metal film detachment.

The invention discloses a single spore isolation method of alternaria alternata, which comprises the following steps: collecting disease tissues infected by the alternaria alternata, and culturing the alternaria alternata; preparing a culture medium for growth of alternaria alternata; melting the end part of the clean glass capillary tube, and stretching the glass capillary tube into a sharp needle shape; under a microscope, a glass needle is used for picking single alternaria alternata spores from the disease tissue subjected to moisturizing culture, the single alternaria alternata spores are transferred into a culture medium to be cultured, and a pure culture is obtained and serves as a basis for purification and identification. According to the method, the borosilicate glass capillary tube is adopted and can be repeatedly burnt, melted and drawn to be manufactured into a key tool glass needle required by single spore separation, materials are effectively saved, meanwhile, the burning and melting process is the sterilization process, and the operation process is saved. By optimizing preparation of a single spore separation tool, the separation efficiency of alternaria alternata can be remarkably improved, the experimental period is shortened, the experimental cost is saved, the success rate of single spore separation is increased, and the single spore separation process of fungi is quicker, more efficient and more accurate.

The utility model relates to an embryonic cell operation mouth suction tube which comprises a mouth suction section at the front end, a connecting section at the middle end and a suction / discharge section at the rear end, the mouth suction section comprises a straight round tube, an inclined tube and a filter, one end of the straight round tube is connected with one end of the filter, and the other end of the straight round tube is connected with one end of the inclined tube; the connecting section comprises a hose, one end of the hose is connected with the other end of the filter, the suction / discharge section comprises a hard connecting pipe, one end of the hard connecting pipe is connected with the other end of the hose, and the other end of the hard connecting pipe is connected with a glass needle. According to the embryonic cell operation mouth suction tube provided by the utility model, scientific researchers can carry out various cell operations by using the mouth suction device under the condition that a mask is not taken off, so that the high maintenance of an experimental environment, the efficient achievement of an experimental purpose and the guarantee of the body safety of the scientific researchers are realized, and finally, the safe operation of a laboratory is guaranteed.

The application discloses a kind of pre-treatment methods of glass needle felt substrate for aerogel, specifically comprising the following steps: first, lay multiple layers of glass fiber to form a first glass fiber layer, then evenly spray inorganic powder on top of the first glass fiber layer to form an inorganic powder layer, and then lay multiple layers of glass fiber on top of the inorganic powder layer to form a second glass fiber layer, forming a glass needle felt substrate with a sandwich structure; finally, immerse the glass needle felt substrate in an aerogel solution for soaking to obtain an aerogel glass fiber material.

The application belongs to the field of aquaculture, and specifically discloses a seawater fish micro-injection needle and a preparation method thereof. The method comprises the following steps: preparing a glass capillary tube and a needle drawing device; drawing the glass needle; breaking the needle; grinding the needle tip; testing the quality of the needle; methyl cellulose treatment; needle storage and use. The micro-injection needle prepared by the method can effectively solve the problems of hard seawater fish egg shell difficult to penetrate, easy breaking of the needle tip during injection, easy sticking of the needle, and great damage to the fertilized eggs, and is suitable for micro-injection research of various seawater fish species, has a wide application prospect, provides important support for gene editing research of seawater fish, and has good application value.

The invention discloses a gold nanowire micro-nano probe as well as a preparation method and application thereof, and belongs to the technical field of nano electrochemical sensors and biomolecule detection. The gold nanowire micro-nano probe comprises a glass capillary substrate, a gold nanowire layer and an insulation sealing layer, the glass capillary base body is provided with a tip area, the tail end of the tip area is provided with an electroactive area, the surface of the tip area is wrapped with a gold nanowire layer, and the surface of the gold nanowire layer is wrapped with an insulating sealing layer except the electroactive area. According to the method, a three-dimensional gold nanowire network is grown on the surface of an insulating glass needle tip in situ through amino silanization modification, gold nanoparticle self-assembly seed crystal and a seed-mediated growth method. The insulating substrate is adopted to reduce background current, and the signal-to-noise ratio is high; the accurate control of the electroactive region is realized through a photoresist patterning process; the preparation process is simple, the cost is low, and the method can be widely applied to the single cell analysis fields of intracellular active oxygen species detection, neurotransmitter detection and the like.

The utility model discloses a special manufacturing tool for a pre-filled syringe glass needle cylinder clamping jaw head body, which comprises a bottom plate fixed on a machine tool workbench through a bolt and a positioning seat fixedly mounted on the bottom plate, one end part of the positioning seat is provided with an opening end for accommodating a columnar end part at the upper end of the clamping jaw head body, and the other end part of the positioning seat is provided with an opening end for accommodating the columnar end part at the lower end of the clamping jaw head body. One side of the open end is provided with a groove part for accommodating the clamping jaw supporting body; a through hole for accommodating the hollow sleeve of the clamping jaw head body is formed in the opening end of the positioning seat, and a fastening nut is screwed at the other end, penetrating through the through hole, of the hollow sleeve; correction holes are formed in the positions, perpendicular to the vertical projection of the bottom plate, of the intersection points of the center lines of the inclined holes of the three clamping jaw supporting bodies. The tool can replace a traditional five-axis machine tool which is manufactured at high cost, manufacturing of multiple batches of clamping jaw heads is completed on the tool at present, the requirement is completely met, the effect is good, and the manufacturing cost is greatly reduced.

The invention provides a vitamin K1 injection package preparation method and a vitamin K1 injection package, the vitamin K1 injection package preparation method comprises the steps of filling, nitrogen filling, plug adding and rod screwing, in the nitrogen filling step, sterile nitrogen is filled into a glass needle tube, oxygen in the glass needle tube is removed to be 2.0% or below, in the plug adding step, the glass needle tube is vacuumized, so that a piston is sucked into the glass needle tube, and the rod screwing is performed. The headspace volume is controlled to be 0.1 to 0.2 ml. According to the present invention, the total impurity amount and the maximum single impurity content of the vitamin K1 preparation in the glass needle tube can be maintained at the low level after the long-term placement, and the potential secondary pollution risk is avoided by using the glass needle tube packaging form.