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67 results about "Sex reversal" patented technology
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Sex reversal is the phenomenon whereby organisms developing at sex-specific conditions such as temperatures or karyotypes hatch the opposite sex. This can be caused by human pollutants, including herbicides, which can act as estrogen promoters or inhibitors, which would respectively increase or decrease the number of female offspring, through controlling aromatase. However, much is still unknown about temperature-dependent sex determination, so the exact mechanism for sex reversal is unknown.
Method for continuously producing complete male pelteobagrus fulvidraco by sex reversion and embryonic development
InactiveCN1994072AShorten breeding timeImprove ergonomicsAnimal reproductionClimate change adaptationNormal femaleFishery
The invention relates to a method for producing male fish, wherein it comprises that: artificially inducing; artificially inducing the female and male fishes; artificial insemination; inverting hormone; feeding seed with Artemia which contains hormone; converting male into biological female; the female grows. The invention comprises sperm inactivation and female diploid generation, to generate ready male fish and normal female fish; crosses the male fish and female fish to obtain the full male fish; inverts the male fish into biological female fish to cross with male fish, to obtain the full super male fish.
Owner:INST OF AQUATIC LIFE ACAD SINICA
Artificial regulatory method for increasing female rice field eel fecundity
InactiveCN101103711AMaintain fecundityClimate change adaptationAnimal feeding stuffFinless eelNatural state
The invention belongs to the aquaculture technology field, in particular relating to an artificial control method for the improvement of female finless eel parent fecundity. The finless eel has special physiological phenomena and sex reversal characteristics with small size, large egg granules and low fecundity. Thus the scale finless eel propagation is subject to the restrictions of the female finless eel parent self-conditions. In the invention, through the artificial simulation of female finless eel culture and development environment, the rational selection of parental finless eels, the reasonable stocking density arrangement, the appropriate feeding and management methods, the pre-oviposition period intensive culture and the artificial control measures, the female finless eel fecundity in natural state can be enhanced to the fecundity up to 450 to 1300 grains each and the 680 grains average level from 170 to 650 grains each and the average 260 grains. Therefore, the average fecundity of finless eel is improved by 161%.
Owner:HUAZHONG AGRI UNIV
Method for establishing full-female pelteobagrus fulvidraco breeding population
InactiveCN103070120AShorten breeding timeShorten generationClimate change adaptationPisciculture and aquariaMatingSex reversal
The invention discloses a method for establishing a full-female pelteobagrus fulvidraco breeding population, which comprises the following steps of: 1) manually hastening parturition and manually inseminating; 2) reversing sex; 3) identifying inheritance sex of the sex reversal pelteobagrus fulvidraco; 4) expanding the population; and 5) establishing the full-female pelteobagrus fulvidraco breeding population, so as to finally establish the full-female pelteobagrus fulvidraco breeding population. According to the invention, an integrating technology of hormone sex reversal combined with pelteobagrus fulvidraco chromosome peculiar molecular markers is adopted, so that XX physiological milter can be confirmed without adopting a test mating method and the breeding time is shortened; compared with the test mating method, one generation can be shortened; the gynogenesis step is omitted, so that the damage to the germ cell genetic material caused by manual induction gynogenesis is reduced; the yield of the XX raun is increased; and the economic and social benefits are obvious.
Owner:WATER ENG ECOLOGICAL INST CHINESE ACAD OF SCI
Method for inducing sex reversal of Banhuashe soleidae fishes
InactiveCN101002546ASuitable temperatureAppropriate hormone concentrationMicrobiological testing/measurementClimate change adaptationHormones regulationPcr method
A method for inducing the sex reversal of half-smooth tongue-sole including temp inducing and hormone inducing is disclosed. Said temp inducing method features that the fry is cultured at 28-32 deg.C for 50-60 days. Said hormone inducing method is characterized by that the fry is immersed in the aqueous solution of methyl testosterone for 50-60 days.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
A kind of breeding method of sex reversal of female carp into male carp
InactiveCN102283149AShorten the breeding cycleLow costClimate change adaptationAnimal feeding stuffPituitary glandCarp
The invention discloses a culturing method for sex reversal of a female carp to a male carp. The culturing method comprises the following steps that A. squeezing an abdomen of a 10-11-month-old carp, and separating a male carp with out-flown semen and a carp with no out-flown semen; B. inserting a copper roe digging device in a genital pore of the carp from which semen can not be squeezed, and selecting the carp capable of obtaining roe particles to cultivate the carp in a cement pond; C. according to the weight of the carp, feeding the carp with dosing fodders containing 17 alpha- methyltestosterone every day, and mix-injecting domperidone, luteinizing hormone releasing hormone and carp pituitary gland in the thoracic cavity of the carp in each five days; and D. after continuously processing the test carp for 2 months, squeezing the abdomen of the carp, the test carp with out-flown milky white semen is the sex reversal carp which is a female on genotype and a male on physiology. The culturing method is easy and feasible and is convenient in operation, and can be used for sex reversing the female carp on genotype into a male carp on physiology. The growth speed of the filial generation cross bred by the sex reversal carp and the common carp is fast and the economic benefits are remarkable.
Owner:INST OF AQUATIC LIFE ACAD SINICA
PCR primer pair used for identifying genetic sex of Pseudobagrus ussuriensis, and rapid identification method thereof
InactiveCN106011300AAll-male parthenogenesisShorten breeding timeMicrobiological testing/measurementDNA/RNA fragmentationZooidPhysiology
The invention discloses a PCR primer pair used for identifying the genetic sex of Pseudobagrus ussuriensis, and a rapid identification method thereof. The identification method comprises the steps of examined individual tissue drawing, genome DNA extraction, sex specific DNA fragment PCR amplification, agarose gel electrophoresis, ultraviolet light detection shooting and examined individual genetic sex determination. High-quality genome DNA is extracted through an optimized process, a sex specific DNA fragment is amplified, and the existence or not and the existence characteristic of the sex specific fragment are detected under ultraviolet lights, so the genetic constitution of Pseudobagrus ussuriensis is identified, and the genetic sex is discriminated in 1-2d. The PCR primer pair and the rapid identification method provide a rapid identification method for identifying sex reversal fish and super-male fish, provide technical support for breeding all-male fish, and have the advantages of breeding time shortening, great increase of the output, breeding cost reduction, and profound economic and social values.
Owner:HUAIYIN TEACHERS COLLEGE
Method for producing yy super-male and xy physiological female common carps
ActiveUS20180317459A1Easy and feasibleThe method is simple and feasibleAnimal reproductionOrganic active ingredientsNormal femaleCommon carp
Method for producing YY super-male and XY physiological female common carps, with which YY-chromosome super-male common carp can be cultivated and androgenetic YY super-male common carp is produced, where microsatellite markers are used for paternity testing and test crossing confirmation. The YY common carp is crossed with normal female common carp to produce the progenies of only male common carp, and without sex identification, the juvenile male common carp with known sex are subjected to artificial sex reversal to produce XY physiological female common carp.
Owner:INST OF AQUATIC LIFE ACAD SINICA
Method for quickly establishing cross-bred XY holandric pelteobagrus fulvidraco
ActiveCN105360031AStable genetic traitsFast growthClimate change adaptationPisciculture and aquariaEconomic benefitsFishery
The invention discloses a method for quickly establishing cross-bred XY holandric pelteobagrus fulvidraco. The method is characterized in that based on mating offspring of pelteobagrus fulvidraco YY physiological female fish and pelteobagrus vachelli XY male fish, cross-bred YY super-male fish is obtained by cultivation through a genetic method so as to mate with common pelteobagrus fulvidraco female fish to produce the XY holandric pelteobagrus fulvidraco. According to the method disclosed by the invention, the breeding period can be obviously shortened; compared with technologies such as Varadaraj and the like, two generations can be shortened; and compared with a method of continuously producing the holandric pelteobagrus fulvidraco by using sex reversal and gynogenesis, one generation can be shortened. Besides, the cross-bred holandric pelteobagrus fulvidraco obtained by the method disclosed by the invention is stable in genetic character, high in growth speed, large, and strong in adaptive capacity to the environment, has cross-breeding advantages and inherits advantages of the holandric fish, so that better breeding and economic benefits can be obtained.
Owner:武汉百瑞生物技术有限公司
Artificial breeding technique of Sarotherodon sp
InactiveCN101664006AClimate change adaptationPisciculture and aquariaAquaculture industrySarotherodon
The invention relates to an artificial breeding technique of Sarotherodon sp for aquaculture industry, which comprises the stages of parent fish selective breeding and culture, egg laying, artificialhatching and larva breeding and culture, wherein the parent fish selective breeding and culture stage comprises parent fish selection and environmental condition control; in the egg laying stage, an induced spawning method and egg laying time control are required to be mastered; an indoor automatic artificial hatching technology is adopted for hatching for 2 to 3 days to produce fish fry, the fishfry flow to a collecting container with water from an upper opening of a hatcher, and sex reversal is performed at the same time; and before breeding fish fry in a pond, pond cleaning, disinfectionand sterilization, control of conditions such as temperature, salinity and dissolved oxygen, raising and other work are required to be done. The technology is scientific and reasonable, low in investment, simple in facilitates and easy to implement, realizes the artificial breeding of Sarotherodon sp, has the advantages of short breeding time, high fish fry quality, high survival rate and the like, is suitable for large scale breeding production and creates greate economic benefits.
Owner:李瑞伟
Sex reversal siRNA of Chinese mitten crab and application of siRNA
InactiveCN109988763AAchieving sexual reversalIncrease success ratePeptidesOther foreign material introduction processesNucleotideInjury mouth
Owner:WEIFANG UNIV OF SCI & TECH
Sustained release medicine for artificially inducing sex reversal of grouper and its preparation method
InactiveCN1628854AAvoid pollutionSolve the shortageOrganic active ingredientsSexual disorderAromatase inhibitorSynthetic rubber
The invention provides a sustained release medicine for artificially inducing sex reversal of grouper and its preparation method, wherein the medicine comprises aromatase inhibitor 1.5-15%, synthetic rubber base material for medical purpose 75-90%, hardening agent 8.5-10%, the process for preparing the medicament comprises mixing homogeneously, dry forming, and refrigerated preservation.
Owner:SUN YAT SEN UNIV
Method for producing gynoecial loaches
InactiveCN103548740AAvoid damageIncrease acquisition rateClimate change adaptationAnimal feeding stuffMatingArtificial insemination
The invention discloses a method for producing gynoecial loaches. The method comprises the following steps: (1) artificial propagation and artificial insemination; (2) sex reversal: reversing most XX male fishes in fries into XX physiological male fishes by taking artemia larvae soaked into a male hormone solution as a bait, remaining XY male fishes unchanged, and removing XX female fishes till male fishes and female fishes can be distinguished; (3) test mating: feeding the XX physiological male fishes and the XY male fishes to a sexually mature state, mating with the XX female fishes respectively, and remaining a parent of which all offspring are XX female fishes, namely, the XX physiological male fishes; (4) reproduction: mating the XX physiological male fishes and the XY male fishes to produce 100 percent XX female fries, and carrying out sex-reversing on a part of the female fries into XX physiological male fishes according to the step (2); (5) establishment of a population: mating the XX female fishes and the XX physiological male fishes to produce 100 percent XX female fishes, and repeating the steps (4) and (5) to continuously produce gynoecial loaches on a large scale.
Owner:武汉百瑞生物技术有限公司
Efficient induction method and microsatellite molecular marker identification method of Nile tilapia pseudo-female fishes
InactiveCN106399293AEfficient inductionShorten the breeding cycleMicrobiological testing/measurementMutant preparationAgricultural scienceRapid identification
The invention discloses an efficient induction method and a microsatellite molecular marker identification method of Nile tilapia pseudo-female fishes. Different doses of estrogen diethylstilbestrol (DES) are used for sex reversal processing of different groups of Nile tilapia fishes to establish the efficient induction method of the Nile tilapia pseudo-female fishes; and a newly-developed microsatellite molecular marker tlp-23-sd and a primer thereof are further used for identification of the Nile tilapia pseudo-female fishes to establish the microsatellite molecular marker rapid identification method of the pseudo-female fishes, wherein the microsatellite molecular marker tlp-23-sd is closely linked with the sex. The efficient induction method is high in pseudo-female fish induction efficiency, and the microsatellite molecular marker rapid identification method is rapid, accurate and reliable in identification, and can be used for the production and identification of the Nile tilapia pseudo-female fishes.
Owner:SUN YAT SEN UNIV +1
Breeding method of all-male hybrid snakehead
ActiveCN110358819AHuge industrial valueGreat scientific valueMicrobiological testing/measurementClimate change adaptationZoologySex reversal
The invention belongs to the technical field of fish breeding, and discloses a breeding method of all-male hybrid snakehead. The method includes the following steps of bred channa maculata sex reversal, identification of pseudo-female fishes, reproduction of the pseudo-female fishes, identification of super-male fishes and expanding propagation of the super-male fishes. By using the method, the all-male hybrid snakehead can be bred, and the male rate is 93% or above; after being bred for 7 months, the all-male varieties are increased by 8% or above in yield compared with normal varieties, andthe all-male varieties with the height of 2 jin or more are increased by 15%; the coefficient of a novel fish feed in the current year of breeding is reduced by 0.1; after being bred for 7 months, theall-male varieties are increased by 26.6% in yield compared with normal varieties, and the feed coefficient is reduced by 0.175-0.21. The new varieties 'hybrid snakehead' obtained through hybridization have the advantages of being high in growth speed and strong in low temperature resistance, can replace snakehead breeding in the north area and become national breeding varieties.
Owner:PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI +1
Artificially induced gynogenesis method for Pseudobagrus ussuriensis
ActiveCN103404461AAvoid toxic effectsIrradiation equipment is simple and easy to operateClimate change adaptationPisciculture and aquariaPlant Germ CellsHigh survival rate
The invention discloses an artificially induced gynogenesis method for Pseudobagrus ussuriensis, comprising the steps of heredity inactivation of sperms, cold shock treatment of germ cells, and hatching; the best time for inactivating the sperms of the Pseudobagrus ussuriensis is found out by regulating the irradiation distance and the irradiation time of ultraviolet; the cold shocks with different time spans are carried out at different time starts after fertilization, the treatment time for a highest survival rate is determined to be the proper time span of the cold shock, and thus the optimal condition suitable for artificially induced gynogenesis of the Pseudobagrus ussuriensis is screened out. According to the technical route of milter sex reversal-artificial gynogenesis-super-milter cultivating-complete milter cultivating, the invention provides a technological base for cultivating the super-milter of the Pseudobagrus ussuriensis and cultivating the monotropic complete milter, so that the cultivation scale is exoanded and the yield is greatly increased, so that the income of farmers is greatly increased, and therefore, the method has a broad industrialized cultivation prospect.
Owner:HUAIYIN TEACHERS COLLEGE
siRNA-IR sequence for sex reversal of male macrobrachium rosenbergii and application of siRNA-IR sequence
ActiveCN110714009AReduce immune stress responseImprove survival rateAnimal husbandryDNA/RNA fragmentationPhysiologySexual differentiation
The invention discloses a siRNA-IR sequence for sex reversal of male macrobrachium rosenbergii and application of the siRNA-IR sequence. Expression of an IR gene is inhibited by a siRNA interference technology to promote sex reversal of the male macrobrachium rosenbergii to obtain neo-females. IR gene fragments of macrobrachium rosenbergii are screened and obtained from macrobrachium rosenbergii transcriptome, and the siRNA is designed and synthesized according to a macrobrachium rosenbergii IR gene sequence, named siRNA-IR. The siRNA-IR is transformed into macrobrachium rosenbergii by a microscopic injection method before a sex differentiation stage to interfere and inhibit expression of the IR gene in the macrobrachium rosenbergii and promote sex interversal of the macrobrachium rosenbergii. Compared with a dsRNA interference technology, the siRNA technology has smaller injection dosage, and can reduce the cost, effectively reduce immunological stress reactions of post-larvae of themacrobrachium rosenbergii and increase the survival rate of neo-females.
Owner:HUAZHONG AGRI UNIV
Ecology regulation and control method for delaying sex reversal time of eels
InactiveCN103004649AImprove group fecundityMeet seedling requirementsClimate change adaptationPisciculture and aquariaHigh densityEcological study
The invention relates to an ecology regulation and control method for delaying the sex reversal time of eels. The ecology regulation and control method for delaying the sex reversal time of the eels is characterized in that under an ecology imitation condition, young eels with the same specification are massively produced, and moreover, with the adoption of a pond net cage breeding method, the young eels obtained in the same year are bred and overwinter to grow into 30-40g 1-full-age eels; and after the overwintering, the 30-40g 1-full-age eels are fed for one year with high protein nutrients at a high density in a pond net cage, wherein the feeding density is 50-80 eels per square meter and the feed protein content is 40% to 42%. Through the method, 2-winter-age female eel parents with great brood amount and an individual specification of more than 100g can be produced; the colony reproductive capacity of the female eel parents can be improved by 200% to 300%; the quantity of the produced young eels is correspondingly improved by 200% to 300%; and therefore, the demand on the large-scale breeding of the young eels can be met.
Owner:YANGTZE UNIVERSITY
Method for massively producing all-female loaches
InactiveCN106259089AShort training periodHigh yieldClimate change adaptationPisciculture and aquariaWeatherfishFish species
The invention discloses a method for massively producing all-female loaches. The method for massively producing the loaches comprises the steps that healthy and good female fishes are screened for backup parents, prenatal intensive cultivation and artificial spawning inducement are conducted, and all-female diploid fries are obtained by adopting a gynogenesis method achieved through heterologous sperm stimulation and cold shock treatment; artemia larvae soaked with a methyltestosterone male sex hormone solution with the concentration of 50 mg / kg are continuously taken as baits for 30 days, and XXo+ female fishes in the loach fries are subjected to sex reversal to be XXo+ physiological male fishes; the full-female diploid normal fish fries mate with the physiological male fishes, and then the 100 full-female loaches are massively produced. Compared with the prior art, the method has the advantages that the full-female loaches are massively produced by combining a gynogenesis technology with a sex reversal technology, and therefore the per mu yield and the culturing benefit are significantly increased; by means of the gynogenesis technology, a pure line can be rapidly established, very rich materials can be provided for genetic breeding, the breeding cycle of the full-female loaches can be shortened, and the gynogenesis technology is suitable for the massive production requirements and easier to apply and popularize.
Owner:FISHERIES RES INST ANHUI ACAD OF AGRI SCI
Method for delaying sex reversal of finless eels
InactiveCN101933471AImprove group fecundityMeet seedling requirementsClimate change adaptationPisciculture and aquariaFinless eelSex reversal
The invention relates to a method for delaying the sex reversal of finless eels, which is characterized by comprising the following steps of: under artificial breeding condition, obtaining a great number of 2-age physically-healthy finless eels of the same specification by adopting soilless aquatic breeding in a cement pit; selecting the 2-age finless eels of the same specification for breeding in the soilless cement pit in the June to November of every year, and adding a small amount of nilestriol into compound feeds for feeding the finless eels; keeping breeding the finless eels in the cement pit and stopping the feeding in the November of the same year to the March of the next year; keeping breeding the finless eels in the cement pit and feeding the finless eels again in the April to the May of the next year; and from 2 weeks before the induced spawning of the finless eels on, stopping adding the nilestriol into the feeds for feeding the finless eels. The method has the advantages of delaying the sex reversal of finless eel parents by more than 6 months, achieving the female rate of 95 to 100 percent, improving the fecundity of female finless eel parents by 18 to 25 percent, increasing the number of fries by 15 to 20 percent and meeting the requirements of large-scale eel breeding on the fries.
Owner:YANGTZE UNIVERSITY
A large scale producing method for all-female mandarin fishes
ActiveCN108377936AAchieve masculinization sex reversalObvious growth advantageClimate change adaptationAccessory food factorsAnimal ForagingFishery
The invention discloses a large scale producing method for all female mandarin fishes. The method includes the following steps: S1, the breeding processing for sex reversal of mandarin fishes: sex reversal of mandarin fishes is performed by adopting the method that bait fishes which are fed with forage mixed with 17alpha-methyl testosterone hormone are ingested by the mandarin fishes in ten days after the mandarin fish fries start feeding and before the differentiation of the sexual gland; S2, the sexual gland identification for the sex reversal individuals; S3, the test cross for the sex reversal individuals; S4, the production for all female mandarin fish fries. The sex reversal of mandarin fishes is performed by adopting the method that the bait fish which are fed with forage mixed with17alpha-methyl testosterone hormone are ingested by the mandarin fishes. The technology of inducing sex reversal of mandarin fishes with high efficiency is established to cooperate with the corresponding biological sex sexual gland microscopic identification technology and the genetic sex test cross identification technology to provide technical support for the control of the mandarin fish sex and the all-female breeding. The sex reversal of the masculinization of 99% of female fishes of mandarin fishes can be realized by the provided method, the female proportion of all-female mandarin fishproduction can reach 100%, and all-female mandarin fishes have obvious growth advantages compared with common breeding mandarin fishes so that the method has an excellent application prospect.
Owner:FOSHAN NANHAI BAIRONG AQUATIC SEED +2
Directional breeding method of female parents of finless eel
InactiveCN104067961AImprove group fecundityMeet seedling requirementsClimate change adaptationPisciculture and aquariaFisheries scienceFinless eel
The invention relates to a directional breeding method of female parents of finless eel and belongs to the technical field of fishery science application. The directional breeding method of female parents of finless eel is characterized by comprising the steps of obtaining one-full-age eels growing consistently and having the individual specification of 30-40g by virtue of breeding in pond net cages under imitated ecological conditions, after overwintering, feeding the one-full-age eels of 30-40g in the pond net cages by high density and with high-protein nutrition for a year, namely the feeding density of 50-80 eels per square meter and the feed protein content of 40%-42%, and postponing the sex reversal of the parents of the eels by more than 12 months, thereby obtaining two winter-age female eel parents having large brood amount and the individual specification of more than 100g; the population fertility of the female eel parents can be improved by 200%-300% and the number of the bred eel seedlings is also improved by 200%-300% correspondingly; as a result, the population fertility of the female eel parents is effectively improved and the requirements of large-scale eel breeding on the seedlings can be met.
Owner:YANGTZE UNIVERSITY
Production method of sex-reversed male loach
InactiveCN107232092AAvoid damageIncrease acquisition rateClimate change adaptationPisciculture and aquariaJuvenile fishCrossover test
The invention provides a production method of sex-reversed male loach. The method comprises the first step of conducting incubation; the second step of conducting temperature regulatory reversion, wherein after loach fry is hatched and out of membrane, and before genital ridge of the loach fry is formed, the water temperature is gradually raised to 27-32 DEG C to cultivate the loach fry to juvenile loach; the third step of conducting male hormone regulatory reversion, wherein after the loach fry is hatched and out of membrane, artemia larva and pellet feed which are treated by a male hormone solution are mixed and continuously thrown through a gradual-transition mode; the fourth step of conducting normal feeding, wherein the water temperature is adjusted to a normal water temperature, then the pellet feed which is not treated by the male hormone solution is thrown, and then XX male loach is removed when the loach is fed to the state that the male loach and the female loach can be distinguished; the fifth step of conducting test cross, wherein XX physiological male loach and XY male loach are fed to be sexually mature, then the XX physiological male loach and XY male loach are mated with XX male loach separately, and then male parent of which the filial generation is 100%XX male loach is screened out, namely, XX sex-reversed male loach. Through a joint effect of the temperature and male hormone, regulatory reversion is conducted, so that the survival rate and the sex reversal rate of the loach fry can reach an optimal state simultaneously, and further the production method of the sex-reversed male loach can be used for conducting scale production of full-female loach.
Owner:武汉百瑞生物技术有限公司
Compositions and methods for regulation of steroidogenesis
Compositions and methods relating to the regulation of transport of cholesterol into the mitochondria of a cell and, therefore, for the regulation of steroidogenesis are provided. Compositions include nucleic acid molecules encoding a steroidogenic acute regulatory protein (StAR), StAR protein molecules and peptides having amino acid sequences as disclosed herein, and anti-StAR antibodies. Methods include immunoassays using anti-StAR antibodies and nucleic acid based screening methods for pathologies correlated with defects in StAR, such as lipoid congenital adrenal hyperplasia and dose sensitive sex reversal. In addition, these compositions and methods may be useful for treatment of steroid hormone-dependent disorders, in particular, for lipoid congenital adrenal hyperplasia.
Owner:TEXAS TECH UNIVERSITY HEALTH SCIENCES CENTER
Anti-mullerian hormone AMH gene for epinephelus lanceolatus, encoding protein and application of anti-mullerian hormone AMH gene
The invention discloses an anti-mullerian hormone AMH gene for epinephelus lanceolatus. A nucleotide sequence of the anti-mullerian hormone AMH gene is shown as SEQ ID NO:1. The invention also discloses protein encoded by the gene, a preparation method for producing anti-mullerian hormone AMH recombinant protein by adopting the anti-mullerian hormone AMH gene as a target gene, and a polyclonal antibody prepared by adopting the anti-mullerian hormone AMH recombinant protein obtained by the method as antigen to immunize animals. The invention further discloses application of anti-mullerian hormone AMH recombinant plasmid to preparation of an inducer in a sex-reversal process of the epinephelus lanceolatus and application of the polyclonal antibody to preparation of detecting agents for different developmental periods of the epinephelus lanceolatus.
Owner:SUN YAT SEN UNIV
Method for cultivating pure aristichthys nobilis red.var groups
ActiveCN103190366AImprove hatchabilityHigh activityClimate change adaptationPisciculture and aquariaBiological activationZoology
The invention discloses a method for cultivating pure aristichthys nobilis red.var groups. The method comprises activating roe of aristichthys nobilis red.var with diploid sperms of allotetraploid crucian carps, performing cold shock treatment after activation, and controlling the temperature through flowing water to obtain gynogenesis aristichthys nobilis red.var; feeding feeds containing male hormone after incubation for several days to obtain sex reversal male gynogenesis aristichthys nobilis red.var; and performing selfing with gynogenesis aristichthys nobilis red.var serving as the female parent and with male gynogenesis gynogenesis aristichthys nobilis red.var serving as the male parent to obtain gynogenesis pure aristichthys nobilis red.var groups. Further, the gynogenesis aristichthys nobilis red.var can serve as the female parent, a common male aristichthys nobilis red.var serves as the male parent, and the female parent and the male parent are hybridized to obtain gynogenesis pure aristichthys nobilis red.var groups. According to the method, the roe hatchability and the survival rate can be improved greatly, and the pure aristichthys nobilis red.var groups stable in body color and excellent in performance can be cultivated.
Owner:HUNAN XIANGYUN BIOLOGICAL
Epinephelus coioides SRY box relative gene SOX and its use
The present invention relates to Epinephelus coioides SRY box relative gene Sox and its application. The present invention finds the gene Sox from the sex gland of Epinephelus coioides and the gene has important application in sex gland development and sex reversal of Epinephelus coioides.
Owner:SUN YAT SEN UNIV
Method for artificially inducing sex reversal of female siniperca chuatsi
InactiveCN112535123AImprove farming outputShorten the breeding cycleClimate change adaptationPisciculture and aquariaZooidAnimal science
The invention discloses a method for artificially inducing sex reversal of female siniperca chuatsi. The method comprises the following steps of (1) breeding siniperca chuatsi parents; (2) conductingartificial spawning induction of the siniperca chuatsi; (3) obtaining heterologous sperms of siniperca scherzeri; (4) conducting genetic inactivation of the heterologous sperms of the siniperca scherzeri; (5) obtaining mature eggs of the siniperca chuatsi; (6) performing artificial gynogenesis on the siniperca chuatsi to obtain female individuals; and (7) artificially inducing sexual transformation of gynogenesis siniperca chuatsi. According to the method, the siniperca chuatsi can be subjected to artificial gynogenesis induction to obtain all-female siniperca chuatsi, then the all-female siniperca chuatsi is induced by artificial male hormone methyl testosterone (MT) to generate pseudo-male fishes with XX genotypes, sperms of the pseudo-male fishes have the same fertilization capacity ascommon male fishes, and a large number of all-female seedlings can be obtained as common female fish parents by the pseudo-male fishes and common female fishes; and the culture yield and the commercial value are greatly improved.
Owner:WUHAN ACADEMY OF AGRI SCI
KNOCK DOWN MODEL OF DICKKOPF HOMOLOGUE 3 (Dkk3) FOR ASSESSING ROLE OF SAID Dkk3 IN SPERMATOGENESIS AND SEX REVERSAL
InactiveUS20120284809A1Increase milk productionIncrease productionMaterial analysisAnimal husbandryDiseaseSpermatogenesis
The present invention relates the function of Dickkopf 3 (Dkk3 ) in testis using shRNA mediated knock down model. Specifically, the present invention provides knock down model comprising reduction in Dkk3 activity. The knock down model of Dkk3 consisting of non human vertebrates which have, incorporated in their genome, shRNA construct targeting mammalian Dkk3 gene exhibits low testis weight, low sperm count and has low litter size. The knock down model displays disrupted seminiferous tubules and are subfertile. The present invention model has a role in sex determination as its interruption leads to sex reversal of XY gonads, converting males to females. Sex reversal role of Dkk3 knock down model can find its utility in agricultural applications. The present invention describes Dkk3 as a dual function protein which is associated with sex determination as well as is essential for the process of spermatogenesis. Such testicular functional studies are useful as a model for various disease states of infertility or subfertility and for identifying a potential treatment to overcome idiopathic infertility.
Owner:NATIONAL INSTUTUTE OF IMMUNOLOGY
Oncorhynchus mykiss all-female triploid large-scale production method
ActiveCN114532259AAchieve scaleHigh magnificationClimate change adaptationPisciculture and aquariaAnimal scienceGermplasm
The invention discloses a rainbow trout all-female triploid large-scale seed production method, and belongs to the technical field of fish sex regulation and control and ploidy breeding. According to the method, a gynogenesis technology based on body color marking is developed, all-female germplasm is created in batches, then the all-female germplasm is induced into sex reversal male fish (pseudo-male fish) in batches through a sex control technology, all-female fertilized eggs are obtained through hybridization of the pseudo-male fish and common female fish, and the sex reversal male fish is obtained through pressure treatment. The pressure and the placing time of the fertilized eggs in the pressure device are limited, and finally the purpose of batch production of rainbow trout all-female triploids is achieved. The method effectively solves the problem of triploid offspring seed supply in the rainbow trout breeding industry, and provides a solution for localization substitution of imported triploid offspring seeds.
Owner:HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
Method of artificially inducing gynogenesis in Ussuri breamus
ActiveCN103404461BExpand the scale of farmingIncrease productionClimate change adaptationPisciculture and aquariaPlant Germ CellsProper time
Owner:HUAIYIN TEACHERS COLLEGE
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