285results about How to "Avoid toxic effects" patented technology

One kind of short range nitrifying-anaerobic aminoxidizing sequencing batch type denitrifying biomembrane mode and plant is disclosed. The limited aerating mode with real-time control of the sewage temperature inside the reactor and high frequency alternation between aeration and anaeration can inhibit the growth of nitrite oxidizing bacteria and denitrifying bacteria so that the aminoxidizing bacteria and the anaerobic aminoxidizing bacteria become the dominant bacteria in the nitrifying and denitrifying stages. In the nitrifying stage, nitrification is stopped in the nitrite step; and in the denitrifying stage, ammonia nitrogen acts as the direct electron donor to reduce nitrite. The plant is provided with fixed spherical composite stuffing with spherical shell of polypropylene and filled inside biological composite material. Sectional discrete water feeding mode is adopted to feed the sewage to be treated in four stages into the reactor.

The invention relates to a coated compound fertilizer, in particular to a totally biodegradable coated compound fertilizer and a preparation method thereof. The coated compound fertilizer consists of a coated layer and a compound fertilizer core. The main body of the coated layer is polylactic acid and / or polybutylene succinate. The coating process adopts fluidized bed spraying-coated technology, and comprises the following steps: dissolving polylactic acid and / or polybutylene succinate in trichloromethane, adding carboxymethylcellulose, urease and / or nitrification inhibitor, organic and / or inorganic conditioner in the solution, spraying and coating the surfaces of the fluidized compound fertilizer granules after uniformly mixing the mixture under the stirring of a stirrer to form the uniform and complete organic polymeric membrane layer. The process has the advantages of easy implementation, and capacity of effectively slowing down the release of the fertilizer nutrient to the outside and the conversion rate of nitrogen in soils, effectively controlling the release and conversion of the fertilizer nutrient in soils, and reducing the pressure on environment caused by the rapid release of nutrient. The coated material can be totally biodegradable, and the degradation products have no secondary pollution to the environment.

The invention relates to a process that sulfureted hydrogen is generated by performing the sulfate reduction to low concentration heavy metal sulphate solution in biological metallurgy through microorganism sulfatereducing bacteria, the selective precipitation of the valuable metallic sulfide is realized through the method of adjusting pH value, the traditional process of separating, concentrating and reutilizing is adopted to extract metal. The process is composed of three separate units: anaerobic acidogenic fermentation, sulfureted hydrogen generated through sulfatereducing bacteria and selective precipitation of the metallic sulfide. The process not only can exert the advantages that the process of the bacteria leaching is simple, the operation is easy, the cost is low and the pollution is low, but also has high recovery ratio of valuable metal, and the post treatment process can be economically and effectively built, in addition, the process also provides novel methods for recycling other low concentration waste water including sulphate.

The invention discloses a fresh-cut lotus browning inhibitor and a preparation method and application thereof, and belongs to the technical field of fresh-cut root vegetable production. The fresh-cut lotus browning inhibitor is characterized in that the fresh-cut lotus browning inhibitor is prepared from food additives such as L-cysteine, ascorbic acid and citric acid and water; and fresh-cut lotus roots are soaked in the inhibitor solution for 8 to 15 minutes, packaged, and can be stored at the temperature of between 4 and 5DEG C for 10 days. The browning inhibitor can keep the original color of the fresh-cut lotus roots, and has the effect equivalent to sulfite; meanwhile, the inhibitor does not contain components harmful to human bodies such as the sulfite, the inhibitor is safe to use.

The invention discloses a manufacturing method and process device of a reactivated cleaning agent for regeneration of a honeycomb SCR (selective catalytic reduction) denitration catalyst. The deactivated SCR denitration catalyst in the process device is regenerated through the steps of pressurized water spraying, ultrasonic cleaning, secondary pressurized water spraying, acid washing, forced air drying, active component supplementation, secondary forced air drying, step-by-step calcination and the like in sequence. By using (NH4)2SO4 as a main component of an acid washing solution, potential hazards caused by sulfuric acid are avoided, and the economic performance and the safety are realized. The components such as EDTA (ethylene diamine tetraacetic acid) are added into the cleaning agent, and under the chelation of the EDTA and heavy metal elements, the toxic action on the denitration catalyst caused by the heavy metal elements can be well eliminated. According to the process, the denitration activity of the deactivated catalyst can be recovered, and meanwhile, the structural strength of the catalyst can be kept. The process device is simple, suitable for regeneration in hoisting transportation, and high in efficiency. By the promotion of the process, the performance of the regenerated honeycomb SCR catalyst can be improved, the replacement cost of the catalyst is saved, and the process has an important significance on NOX control.

The invention discloses an advanced treatment method for production waste water of an acrylic acid and ester device, belonging to the technical field of treatment of organic waste water. A combined treatment process consisting of aldehyde removing treatment, coagulating and depositing two-section pretreatment, anaerobic treatment, aerobic biological secondary treatment, ozone catalytic oxidation and active carbon advanced treatment is adopted, so that the problem of advanced treatment of the production waste water of the acrylic acid and ester device is solved, and effluent can reach a national first class discharge standard of Comprehensive Sewage Discharge Standard (GB8978-96). In the combined process, biological treatment is taken as a main body, so that treatment cost on waste device of the acrylic acid and ester device can be reduced greatly.

The present invention discloses a preparation method of a cationic organosilicon modified waterborne PUA fabric coating adhesive with a self crosslinking structure, the method is as follows:(1)using polyisocyanate and hydroxyl-terminated polysiloxane for synthesis of an-NCO-group-terminated prepolymer;(2)adding polyether or polyester glycol for reaction;(3)reacting with a cationic hydrophilic chain extender for a period of time, and further reacting with adipic dihydrazide(ADH)to obtain a hydrazine-terminated cationic waterborne polyurethane emulsion;(4)using acrylate monomers and diacetone acrylamide(DAAM)for preparing a hydrazide-group-containing polyacrylate emulsion; and(5)mixing the hydrazine-terminated cationic waterborne polyurethane emulsion and the hydrazide-group-containing polyacrylate emulsion in a certain proportion to obtain the self-crosslinking cationic organosilicon modified waterborne PUA fabric coating adhesive. The self-crosslinking cationic organosilicon modified waterborne PUA fabric coating adhesive avoids the use of a harmful crosslinking agent, has the advantages of being environmental-friendly, high in water pressure resistance, good in mechanical properties, low in cost, and dyeable and the like, and can be used for coating finishing of nylon fabrics.

The invention provides a culture liquid for promoting ectogenesis of embryo vitrified by an open pulled straw (OPS) method after thawing. Frozen 2-cell embryo after thawing is cultured in the culture liquid containing 10<-9> mol / L of melatonin (MT), so that the blastula development rate of the embryo is improved and the number of the cells in the embryo is increased, thereby improving the embryo quality, and providing a beneficial reference for further developing the study of OPS cryopreservation of livestock embryos.

The use of classical superantigens for treatment of cancer has resulted in a low response rates and serious toxicity in humans which is attributable, in part, to the presence of preformed superantigen specific antibodies in the plasma of treated patients. The present invention addresses this problem by providing a method for treating tumors comprising the administration of one or a plurality of egc (enterotoxin gene cluster) staphylococcal enterotoxins comprising staphylococcal enterotoxins G, I, M, N, O. These superantigens in native unmodified form can be administered intrathecally, intratumorally, intravenously to humans with advanced lung cancer while resolving pleural effusions and prolonging survival to 300% above control patients treated with talc pleurodesis. Intratumoral egc superantigens induces a significant and sustained reduction of the tumor size. In contrast to classic Sags, the egc superantigens induced minimal toxicity, are rarely associated with the presence of preformed antibodies and are used as a plurality with a broad T cell Vβ profile. Useful egc superantigen compositions for parenteral administration native egc enterotoxins, homologues, fragments and fusion proteins of native egc enterotoxins capable of activating a broad spectrum of T cells expressing T cell receptor / α motifs. T cell survival-enhancing cytokines IL-7, Il-15, Il-23 are used. together with parenteral egc SE therapy. Also disclosed is combined therapy that includes parenteral, intratumoral or intrathecal superantigen compositions in combination with (i) intratumoral low, non-toxic doses of one or more chemotherapeutic drugs or (ii) systemic chemotherapy at reduced and non-toxic doses of chemotherapeutic drugs or (iii) radiation therapy or (iv) anti-angiogenic and tyrosine kinase inhibitors.

The invention discloses a method for generating 5-hydroxymethylfurfural by using ionic liquid catalysis, which comprises the following steps: dissolving a reaction substrate into an organic solvent, and adding ionic liquid in an amount of 5 to 20 percent based on the total mole of the reaction substrate; and reacting the mixture for at least 2 hours at the temperature of between 70 and 120 DEG C under the protection of inert gas, namely neutralizing the reaction solution, and filtering, drying and distilling the reaction product to obtain the 5-hydroxymethylfurfural. The reaction system shows the characteristic of homogeneous reaction during catalytic dehydration, and has high catalytic activity and selectivity; and the using amount of a catalyst can be optimized to trace so as to improve the reaction conversion rate, reduce the use of other organic solvent and additive, reduce the cost and facilitate the separation and purification of the reaction product at the same time.

A detection reagent kit for overall 25-hydroxy-vitamin-D is characterized by comprising a reagent 1, a reagent 2 and a reagent 3; the reagent 1 is dissociation liquid containing surfactant, the reagent 2 is formed by putting antibodies resisting human 25-hydroxy-vitamin-D2 and 25-hydroxy-vitamin-D3 in a proper buffer solution, and the reagent 3 is formed by putting polystyrene latex particles with the surfaces marked with human 25-hydroxy-vitamin-D2 and 25-hydroxy-vitamin-D3 in a proper buffer solution. The reagent kit has the advantages of being high in detection sensitivity and accuracy, good in precision, wide in detection linearity, good in stability, short in detection time and low in production cost.

The invention discloses a low-expression CYP7A1 hepatic cell and a constructing method thereof. A method for inhibiting the CYP7A1 expression level of the hepatic cell comprises the step of introducing an encoding gene of small-interference RNA which is used for inhibiting the CYP7A1 gene expression into the hepatic cell so as to inhibit the CYP7A1 expression level of the hepatic cell. A slow virus vector system can be used for introducing the encoding gene of small-interference RNA which is used for inhibiting the CYP7A1 gene expression into the hepatic cell. An experiment proves that the method of the invention can obviously regulate the expression level of the CYP7A1 gene of the hepatic cell in an mRNA level and a protein level downwards and can further effectively reduce the secretoryvolume of total bile acid. The Low-expression CYP7A1 hepatic cell and the constructing method thereof can be used for the fundamental research and the clinical application of biologic artificial liver supporting treatment and have a wide application prospect.

The invention discloses an antibiotic peptide buforin II and porcine INF-alpha fusion expression pichia pastoris (Pichia pastoris FZM2009, CCTCC NO:M209259), and a preparation method and applications thereof. The method comprises the steps: A, the construction of expression cassette PAOX1-alpha Factor-IFN alpha-6xHis-bufforin II-Zeocinr; B the construction of expression engineering bacteria Pichia pastoris FZM 2009, wherein the engineering bacteria is resulted from the screening of antibiotics Zeocin and PCR identification; C, the preparation of fusion protein, which comprises the steps: 1) the recovery is implemented on a YPD plate containing 100mug / ml of Zeocin; 2) single clone is inoculated to a YPD-containing culture medium for vibration and culture; 3) 1 volume of culture is inoculated into 10 volumes of a fermentation culture medium for culture; 4) supernatant is obtained by centrifugation; 5) loading buffer with double volume is added into the supernatant, and electrophoresis detection is performed; 6) the supernatant is subject to a molecular sieve gel column to collect components; 7) the collected components are lyophilized to result in fusion protein; 8) buforin II and IFN alpha are obtained by the enzyme digestion of enterokinase on the fusion protein. The fusion protein buforin II and IFN alpha in fusion expression are applied to pig feeds. The method has great feasibility, simple and convenient operation, high expression, low cost and strong bioactivity.

The invention relates to a method for producing a selenium-rich purple wheat by using a biological selenium-rich fertilizer and a whole course dynamic gradient selenium-rich method, which belongs to the crops cultivation technical field. The method is characterized in that the biological selenium-rich fertilizer is produced, and is used to prepare a selenium-rich fertilizer mother liquor, and then the selenium-rich fertilizer mother liquor is diluted to a selenium-containing fertilizer liquid with different selenium concentrations, and is affected on soil, purple wheat seeds and whole purple wheat at different growth periods. The wheat seeds enable full enrichment on the selenium element, according to the growth characteristics of the purple wheat, the selenium-containing fertilizer liquid with different gradients is used for fitting with the purple wheat growth rules during the whole growth process of the purple wheat, thereby the exogenous inorganic selenium can be conversed to plant endogenous organic selenium with high efficiency. By detecting, the selenium content of the purple wheat product provided in the invention is 0.18-0.28mg / kg, wherein the organic selenium content is 0.16-0.25mg / kg, compared with the purple wheat product which is sprayed by a single selenium-containing fertilizer liquid c in the whole process, the selenium content multiple can reach 5-7 times.

The invention relates to a method for extracting high purity anthocyanin. According to the method, purple tomatoes and nightshade are utilized as raw materials respectively. The purple tomatoes and the nightshade are smashed and homogenated first and then are mixed with acid water with the pH of 1 and undergo supersonic extraction and filtration, filter liquor is adsorbed by macroporous resin, impurities are removed, the acid with the pH of 1 and 95% ethyl alcohol are utilized to perform elution, and obtained elution liquid undergoes mist spray and drying by a spray dryer to obtain high purity anthocyanin powder. According to the method, the problems that the loss is large, the yield is low, and the purity is low in the extracting process of plant anthocyanin are solved, the purity of the obtained purple potato anthocyanin reaches up to 54%, the yield accounts for 0.48% of fresh weight, the purity of nightshade anthocyanin reaches up to 56%, and the yield reaches up to 1.3% of the fresh weight. The problem that the purity of the plant anthocyanin sold in the domestic market is generally lower than 24% is solved, the device is simple to operate, the production process is clean and harmless, the product quality is stable, and the method is suitable for industrial production.

The invention provides a preparation method, application and medicine composition and preparation of specific egg yolk immunoglobulin (IgY) and acinetobacter baumannii, as well as a preparation and a kit. The preparation method is characterized by comprising the following steps of: preparing antigen liquor, immunizing hens, and separating and purifying the IgY. In-vivo and in-vitro experiments prove that the prepared specificity IgY has specific binding activity to the acinetobacter baumannii, can be used for effectively inhibiting the growth of the acinetobacter baumannii and alleviating a toxic effect generated by the acinetobacter baumannii, can be applied to prevention, treatment and diagnostic analysis on infectious diseases and complications of the acinetobacter baumannii, and has wide application prospect.

The present invention discloses a spherical alumina carrier material, said carrier material contains 50-90 wt% of alumina and 1-50 wt% of magnetic granules, in which the described magnetic granules are formed from SiO2 convering layer and one or several kinds selected from Fe3O4, Fe and gamma-Fe2O3 as internal kernal material, and the weight ratio of the described covering layer and internal kernel is 0.01-6:1. Said invention also provides its preparation method and application as catalyst carrier.

The invention discloses a gene for coding a glutamine dipeptide biosynthetic enzyme and application thereof. The nucleotide sequence of the gene is shown as SEQ ID NO.1. The invention further discloses an amino acid sequence coded by the gene, and provides a recombinant vector containing the gene, recombinant escherichia coli, and a method for performing biotransformation to synthesize the glutamine dipeptide by using the gene. The gene and the application of a recombinant bacterial strain of the gene have the advantages of high mol conversion rate, high reaction speed, easy separation, low cost and the like. In the synthesis method, the maximum mol conversion rate of the glutamine dipeptide can reach 83.3 percent; meanwhile, the thalli can be applied to the catalytic synthesis of the glutamine dipeptide again after the circulation recovery; in addition, the catalytic activity is stable. Therefore high market competitive power and application values are realized; a foundation is laid for the industrial production of the glutamine dipeptide.

The invention discloses a recombinant blue-green algae, which is prepared by a method comprising the step of introducing genes related to butanol synthesis shown in (1) and (2) into yhe chroococcaceae blue-green algae, wherein (1) the genes related to butanol synthesis comprise encoding genes of the following enzymes: electron transfer flavoprotein A subunit, enoyl hydrase, butyryl-COA dehydrogenase and alcohol dehydrogenase; and (2) the genes related to butanol synthesis comprise encoding genes of the following enzymes: electron transfer flavoprotein B subunit, the enoyl hydrase, the butyryl-COA dehydrogenase and the alcohol dehydrogenase. The invention realizes the blue-green algae to transform inexhaustible solar energy and greenhouse gas carbon dioxide on the earth into the energy product butanol, avoids wasting grains and other organic matters in the energy regeneration process, and at the same time is beneficial for environment protection. Therefore, one of ideal methods for realizing the sustained and healthy development of clean and renewable energy sources is to utilize the recombinant blue-green algae of the invention to produce the butanol.

The invention provides a method for improving the activity of nitrification function microbiology in activated sludge through directly feeding Fe ions, which comprises a cultivation stage of the nitrification function microbiology and a long-time running activity maintenance stage. In a saprobiont denitrification system, ferric salt chemical agent with Fe <2+> or Fe<3+> is directly fed into an activated sludge mixture of an aerobic pond of a biology denitrification system, and basic compounds are also fed simultaneously; and in the long-time running activity maintenance stage of nitrificationfunction microbiology, the feeding amount of the ferric salt chemical agent with the Fe <2+> or the Fe<3+> is gradually reduced, and the fed ferric salt chemical agent with the Fe <2+> or the Fe<3+> is only used for replenishing loss amount of the Fe<3+> or the Fe <2+>. According to the method, the biochemical reaction metabolic activity of the nitrification function microbiology can be effectively improved, the breeding of the nitrification function microbiology and the secretion of endoenzyme of microbial cell are promoted, the applicability of saprobiont denitrification technology is improved, the bottleneck problems existing in the saprobiont denitrification system are solved, and the low temperature resistant capacity of the system is obviously improved.

The invention discloses a double-functional fusion protein based on an antibacterial peptide, a preparation method and an application thereof. The double-functional fusion protein based on the antibacterial peptide is a fusion protein based on a cecropins B protein sequence and a human epidermal growth factor; the linking protein is especially designed to be a locus which can be identified by thrombin; and a purified His tag is constructed on the fusion protein. In the invention, the biological preparation for the fusion protein is realized by using a prokaryotic expression system and the purified fusion protein is obtained. A bacteriostasis test and a verification for realizing live animals are performed by the purified fusion protein to indicate that the fusion protein can be applied to preparing a medicine for treating epidermal wound, can be applied to diseases such as epidermal wound, abrade, burn and scald, etc., and can prevent wound from being infected.

The invention discloses a method for preparing a polycarboxylic acid water reducing agent by using sodium methyl acryl sulfonate as a chain transfer agent. The method comprises the following steps of: firstly, adding water, sodium methyl acryl sulfonate and modified polyether in a reaction kettle; then mixing sodium methyl acryl sulfonate and acrylic acid with water to prepare an acrylic acid solution, mixing water with ammonium persulfate to prepare an ammonium persulfate solution; when the temperature of materials reaches 60 DGE C+ / -2 DEG C, simultaneously dropwise adding the ammonium persulfate solution and the acrylic acid solution; and carrying out heat insulation reaction for 1-3 hours and when cooling below 45 DEG C, adding 32% liquid caustic soda for neutralization so as to obtain the polycarboxylic acid water reducing agent having the PH value of 6-7 and 40% of solid content. Because sodium methyl acryl sulfonate is used as the chain transfer agent instead of mercaptoacetic acid or mercaptopropionic acid in the preparation process of the polycarboxylic acid water reducing agent, environment pollution and toxic action on a human body caused by stink odor and strong corrosivity of mercaptoacetic acid or mercaptopropionic acid can be avoided.

The present invention aims at synthesizing one kind of nanometer pH sensor with embedded reference dye and sensitive dye and stable performance for non-invasive monitoring of dynamic pH variation inside cell. The preparation process includes mixing oil phase cyclohexane, surfactant Triton X-100 and co-surfactant n-hexanol and water as dispersive phase to obtain water-in-oil micro emulsion; adding mixed sensitive and reference dye liquid via stirring; adding ethyl acetate as sylylation reagent, TEOS and catalyst ammonia water to react, demulsifying with acetone, washing with absolute ethanol and super-purified water to obtain inner reference nanometer sensor with silicon shell. The inner reference nanometer sensor is used in non-invasive dynamic intracellular pH monitoring.

The invention provides a formaldehyde-free fixative for a tissue sample, which comprises the following ingredients in parts by volume: 710-790 parts of ethanol, 20-40 parts of glacial acetic acid, 10-30 parts of acetone, 40-60 parts of polyhydric alcohol, 40-60 parts of trichloroacetic acid (TDA), 40-60 parts of surface active agent and 40-60 parts of solubilizing agent. The formaldehyde-free fixative is a nontoxic and innocuous chemical reagent; after being proved by cellular morphological experiments, immunohistochemical experiments, molecular pathological experiments and nucleic acid extraction experiments, the formaldehyde-free fixative has relatively comprehensive fixing effect, can replace the formaldehyde and be used in related pathological experiments, thereby fixing the tissue sample.

The invention discloses a method for treating rubbish percolates of different times for deep denitrification by using a bipolar USAB+A / O+SBR process, which belongs to the technical field of biological denitrification and is suitable for performing advanced treatment on the rubbish percolates. In the method, water output by an A / O reactor passes through a secondary sedimentation tank, then part of the water flows back into a first-stage UASB reactor to perform denitrification, so the method fully uses sufficient carbon sources of raw water, realizes an efficient denitrification efficiency, and fully uses the characteristic that the rubbish percolates contain high-concentration organic substances. Dissolved oxygen (DO), an oxidation-reduction potential (ORP) and a pH value sensor are used for monitoring the degradation and the short-range nitrification / denitrification processes of the organic substances in the reactor, and the short-range nitrification / denitrification processes can be well controlled according to monitored pH values and ORP values. The average ammonia nitrogen concentration of final yielding water is 2 mg / L, the total nitrogen concentration is lower than 40 mg / L, and the ammonia nitrogen removal rate and the total nitrogen removal rate are above 98 percent. The method completely depends on biological treatments, reduces the cost and simplifies the process. The method performs denitrification on the rubbish percolates, not only realizes complete nitrification but also realizes short-range nitrification, and realizes the deep removal of the nitrogen in the rubbish percolates.

The invention discloses a bio-polyamines-modified graphene adsorption material for selectively adsorbing phenol in mainstream smoke of cigarettes as well as a preparation method and application of the bio-polyamines-modified graphene adsorption material. The bio-polyamines-modified graphene adsorption material is prepared from the following raw materials in parts by weight: 1 part of graphite powder, 5-50 parts of inorganic acid, 30-100 parts of strong oxidative substance, 5-20 parts of bio-polyamines and 0.3-1 part of 1-ethyl-(3-dimethylaminoprpyl) carbodiimide. The preparation method comprises the following steps: firstly preparing the graphite powder into a crude carboxylated graphite product, then adding polyamines and EDC, and purifying to obtain a spermine modified graphene adsorption material. The spermine modified graphene adsorption material is added into a cigarette with a cellulose acetate filter tip when used. According to the bio-polyamines-modified graphene adsorption material as well as the preparation method and the application thereof, modified graphene modified with a functional polar group is firstly used in the field of reduction of tar and harm of tobaccos, has the adsorption efficiency far higher than the traditional bulk-phase adsorption material and a three-dimensional nanometer adsorption material and is capable of greatly improving the clearing efficiency for harmful substances in the mainstream smoke of the cigarette.

The Chinese medicine preparation, Fengshi'an, for treating rheumatism has the effective component 3-O-alpha-L-pyranorhamnose (1->2)-beta-D-xylopyranose-oleanolic acid-28-O-alpha-L-pyranorhamnose(1->4) beta-D-pyranoglucose (1->6) beta-D-glucopyranoside, named flaccid anemone saponin W3, and with molecular expression of C59H96O25. Flaccid anemone saponin W3 is prepared with coarse flaccid anemone rhizome powder, and through reflux extraction, concentration, adsorption and elution in macroposrous resin, adsorption and elution in dry silicon gel column and liquid separation. Flaccid anemone saponin W3 is further prepared into Chinese medicine preparation with flaccid anemone saponin W3 content over 90 wt%, and the Chinese medicine preparation is used in treating rheumatic diseases with high curative effect and less toxic side effect.

A special organic compound modifier used in carbonate-type paddy fields and a preparation method thereof relate to a special organic compound modifier used in paddy fields and a preparation method thereof. The modifier and the preparation method thereof solve the problems of soil hardening easily caused by modifiers used in the carbonate-type paddy fields, worsening of physicochemical properties,poor effect on reducing soil alkalinity and high preparation cost. The modifier is made from the following materials by mass percentages: 50-60% of furfural residue, 5-12% of furnace ash, 30-35% of animal manure and 3-7% of brown coal. The preparation method comprises the following steps: blending the furfural residue and the furnace ash to obtain a mixture; mixing the animal manure and the browncoal for fermentation, and obtaining an organic fertilizer; mixing the mixture and the organic fertilizer for fermentation, and then sequentially granulating, ball blasting, sieving and packaging, thus obtaining the modifier. The modifier contains 60-70% of organic matter with pH of 3-4, and has advantages of convenient use, easy mechanical application at large area, simple preparation process andlow cost of raw materials.

The invention discloses a lagerstroemia plant stem tip chromosome tablet preparation method which comprises the following steps of: selecting a part with a length of 0.5-1.0 cm at the innermost side of a lagerstroemia plant annual branch stem tip growing point or a newly-grown stem tip growing point after a branch which is pruned or refrigerated in early winter is subjected to water culture, fixing with stationary liquid (Ethanol: glacial acetic acid: chloroform is equal to 5:3:2 according to the to volume ratio), carrying out hypotonic treatment, carrying out hypotonic treatment again after mixed enzyme liquid is subjected to enzymatic hydrolysis and preparing a tablet by adopting a flame drying method. The chromosome tablet preparation method has the beneficial effects that the processes of pretreatment, dissociation and chromosome tabletting are omitted, the tablet preparation efficiency is improved through directly fixing, the toxic effect of pretreatment liquid and dissociation liquid on a chromosome is avoided, the original form of the chromosome is maintained, the better chromosome dispersion effect is obtained, and meanwhile, the sampling range and the time of lagerstroemia plant stem tip chromosome preparation are enlarged. The tablet prepared by the method can be used for a fluorescence in-situ hybridization experiment to lay a good foundation for the further research of a lagerstroemia genome.

The invention belongs to the field of cell cryopreservation and particularly relates to a periodontal ligament stem cell cryoprotectant and a cryopreservation method thereof. The cryoprotectant disclosed by the invention is prepared from glycerinum, mesenchymal steam cell serum-free medium and human serum albumin. The cryoprotectant disclosed by the invention can effectively solve the technical defects that an existing cryoprotectant has low use effect and a survival rate of post-resuscitation periodontal ligament stem cells is low. The periodontal ligament stem cell cryoprotectant disclosed by the invention can keep activity of cryopreserved cells; furthermore, the survival rate of the post-resuscitation periodontal ligament stem cells is obviously improved, and expression of surface markers of the stem cells and differentiation potential of the stem cells are not affected.