8results about How to "Avoid toxic effects" patented technology

The invention provides an arsenic hyperaccumulation double-gene recombinant vector and application of the arsenic hyperaccumulation double-gene recombinant vector in repairing an arsenic polluted environment, and belongs to the technical field of gene engineering. The arsenic hyperaccumulation double-gene recombinant vector takes a plant expression vector containing a pUbi promoter as a skeleton vector, and further comprises a PvACR3 gene and PvACR3, 1 gene and a prbcS promoter; wherein the pUbi promoter is used for promoting the expression of the PvACR3 gene; a prbcS promoter is used for starting the PvACR3; 1 gene expression. Aiming at the problem of rice death caused by high expression quantity of arsenic accumulation genes in transgenic rice in the prior art, through reasonable design of insertion genes and combination of promoters, toxicity of arsenic accumulation to the transgenic rice is avoided, and the arsenic removal quantity of the transgenic rice is greatly improved.

The invention belongs to the technical field of rubber products, and provides silicone rubber for a composite insulator, a preparation method of the silicone rubber and the insulator, and the preparation method comprises the following steps: S1, mixing methyl vinyl phenyl silicone rubber and glycidyl methacrylate grafted silicone rubber in parts by weight, adding fumed silica and modified magnesium hydroxide, and uniformly stirring to obtain a silicone rubber mixture; continuously mixing, adding silicone oil, triethanolamine and a vulcanizing agent, stirring and mixing, and standing to obtain a mixed rubber material; s2, the mixed rubber material is put into an open mill to be mixed, a vulcanizing agent is added, thin passing and sheet discharging are conducted, standing is conducted, and rubber sheets are obtained; s3, putting the rubber sheet into a mold for molding, and then carrying out vulcanization reaction to obtain the silicone rubber material. The silicone rubber material provided by the invention has excellent mechanical property, flame retardant property, anti-aging property and electrical insulation property. Meanwhile, the preparation process is simple, raw materials are easy to obtain, cost is low, and wide application prospects are achieved.

The invention provides a polyoxyalkylene ether macromonomer, a preparation method and application of the polyoxyalkylene ether macromonomer in preparation of a polycarboxylate superplasticizer, the polyoxyalkylene ether macromonomer is obtained by performing ring-opening polymerization reaction on an initiator and an alkylene oxide monomer under the action of a deprotonation reagent, and the initiator is allyl mercaptan or methyl and phenyl substituted allyl mercaptan. The polycarboxylate superplasticizer is obtained by free radical copolymerization reaction of an acid monomer and the polyoxyalkylene ether macromonomer under the action of an initiator and a reducing agent. Allyl sulfide is easy to generate uniform cracking of carbon-sulfur bonds in the polymerization process, polyoxyalkylene ether sulfydryl free radicals are generated, chain transfer is achieved, in the chain transfer process of the polyoxyalkylene ether macromonomer, a transferred chain still retains terminal double bonds and can participate in polymerization again, and the polyoxyalkylene ether macromonomer has a good polymerization effect. Therefore, polycarboxylic acid synthesized by using the polyoxyalkylene ether macromonomer has a certain branched topological structure, and cement paste dispersed by the polyoxyalkylene ether macromonomer has better workability.

The invention relates to the technical field of plant tissue culture, in particular to application of amphotericin B in inhibition of endophyte pollution of ancient cinnamomum camphora stem tissue culture. It is found that amphotericin B can effectively inhibit endophyte pollution in the ancient cinnamomum camphora stem tissue culture process, meanwhile, it is avoided that high-concentration antibiotics generate a toxic effect on stem induction, the stem induction endophyte pollution rate in the tissue culture process is effectively reduced and can be reduced to 6%, the stem induction rate can still reach 78%, and the tissue culture cost is reduced. The bottleneck problem of endophyte pollution in the ancient cinnamomum camphora stem tissue culture process is solved, and technical support is provided for efficient propagation and preservation of precious germplasm resources of ancient cinnamomum camphora.

The invention provides a culture of aspergillus terreus in an iron-limited culture medium and application of the culture in organic pollutant treatment, and relates to the field of microorganisms and environmental remediation. According to the method, an iron-limited culture environment is adopted, aspergillus terreus is induced to secrete an iron carrier with metal chelating capacity, an iron source is captured, and therefore an efficient Fenton-like reaction system is constructed, generation of hydroxyl free radicals is promoted, and the method is suitable for in-situ green remediation of refractory organic pollutants.

The invention discloses a fertilizer for eliminating continuous cropping obstacles of American ginseng soil as well as a preparation method and application of the fertilizer, and belongs to the technical field of anti-stress preparations and fertilizers. According to the invention, the mixed bacterial liquid is loaded in the pore structure of the porous carrier, and organic and inorganic fertilizers are combined to prepare the fertilizer capable of eliminating the continuous cropping obstacle of American ginseng soil. The obtained fertilizer can achieve the following effects: (1) the pH value of soil is increased, the problem that the pH value of the soil is reduced due to continuous cropping is solved, and the growth of American ginseng is promoted; and (2) the porous carrier can adsorb phenolic acid substances in root exudates of the American ginseng through physical and chemical effects, so that the phenolic acid substances are prevented from generating toxic effects on the American ginseng, the growth of the American ginseng is promoted, and continuous cropping obstacles are eliminated. And (3) improving the microbial community structure and composition, further promoting the growth of American ginseng, and increasing the yield of American ginseng.

The application belongs to the technical field of sporogon culture, and particularly relates to a culture method of sporogon of Brychus based on a liquid culture medium, which comprises the following steps: S1, preparing a Brychus sample with mature sporogon; S2, preparing a spore suspension, taking a mature sporangium of the Brychus and taking out spores, and dispersing the spores into distilled water to prepare a spore suspension; and S3, inoculation, inoculating the spore suspension into a culture bottle containing a liquid culture medium, and then culturing the culture bottle after being covered. The application customizes a special liquid culture medium formula according to the physiological characteristics and growth requirements of the sporogon of the Brychus, and matches highly adaptive culture environment parameters, and the two synergistically ensure the smooth culture of the sporogon of the Brychus from the two dimensions of nutrient supply and environment adaptation.

The utility model provides a kind of anti-bending breakage's front suspension instrument, belong to ophthalmology relevant treatment upper eyelid ptosis technical field, including anti-bending breakage suspension line, both ends of the anti-bending breakage suspension line are provided with metal guide needle.The utility model solves the risk of bending point fracture of the existing front suspension instrument when in use, suspension line is implanted into human body for a long time, is easy to cause damage to patient after fracture, reduces the safety of instrument use, and the difficulty of instrument production process is greater, may lead to the shedding of suspension line or the abrasion of suspension line by metal guide needle tail end even fracture in the process of instrument use, reduces the practicability of instrument problem.