6 results about "Sporangium" patented technology

The application discloses application of 5,8,11-eicosatrienoic acid in prevention and treatment of plant fungal diseases and belongs to the field of pesticide chemistry. The 5,8,11-eicosatrienoic acid mainly prevents and treats diseases caused by the oomycete class capsicum phytophthora, the compound is a long-chain unsaturated fatty acid, has an antifungal disease effect and can be used as a fungicide to prevent and treat plant diseases. The compound can effectively prevent pathogenic bacteria from invading plants by inhibiting mycelium growth, enhancing cell membrane permeability and interfering with the spore sac release process, and is further used for preventing and treating huge plant fungal diseases. Biological tests show that the compound has a significant concentration-dependent inhibitory effect on the mycelium growth of the capsicum phytophthora, and the half effective inhibitory concentration (EC50) is 60.2 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is more than 83%, the 5,8,11-eicosatrienoic acid can effectively inhibit the release of the capsicum phytophthora spore sac, and the EC50 is 156.3 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is morethan 77%.

The application concerns a microbiological system supporting the consolidation of sand and sandy soils through biocementation, utilizing a mixture of bacteria from the Sporosarcina genus, including Sporosarcina sp. ANT_H38, deposited in the Polish Collection of Microorganisms (PCM) at the Institute of Immunology and Experimental Therapy of the Polish Academy of Sciences in Wrocław under number B / 00547, and Sporosarcina pasteurii DSM 33. The goal is to stabilize and strengthen soil structures through natural mineralization, which increases the substrate's load-bearing capacity. The process involves culturing the bacteria on LB medium, diluting them and combining them with urea, and then applying them to sand or soil with the addition of a cementing medium. The system also operates effectively at low temperatures, enabling broad applications in construction and environmental protection. The application also concerns a method for producing the microbiological system and a method for supporting the consolidation process of sand and / or sandy soils using this system.

The application discloses a culture method for propagating Brachythecium populeum, and belongs to the technical field of Brachythecium populeum tissue culture. The application uses sporangium as an explant to propagate Brachythecium populeum, and quickly obtains Brachythecium populeum spores with a high germination rate, establishes a regeneration system of Brachythecium populeum, lays a foundation for large-scale propagation of Brachythecium populeum, shortens the propagation cycle of Brachythecium populeum, and better maintains excellent properties. The application first establishes a method for culturing Brachythecium populeum by using spores, fills the blank of the prior art, and provides a technical basis for propagation, seed preservation, preparation of protoplasts, recombination experiments and gametophyte culture of Brachythecium populeum.

This invention discloses an arginine methyltransferase protein from *Phytophthora* plant pathogenic oomycetes, its encoding gene, and its applications. The arginine methyltransferase protein provided by this invention is shown in sequences 6-8; its encoding gene is shown in sequences 1-3. Experiments have demonstrated that the protein of this invention plays an important role in the growth, development, and pathogenicity of *Phytophthora capsici*, a typical plant pathogenic oomycete. The absence or reduced expression level of this protein leads to slower mycelial growth rate, reduced sporangium production, decreased zoospore numbers, and reduced pathogenicity of *Phytophthora capsici*. Corresponding arginine methyltransferase inhibitors exhibit varying degrees of activity against typical plant pathogenic oomycetes such as *Phytophthora capsici*, *Phytophthora soybeani*, and *Phytophthora pathogenica*. The gene provided by this invention has significant application potential in the prevention and control of crop blight caused by *Phytophthora* plant pathogenic oomycetes. Novel fungicides developed based on this protein as a target have important practical significance for controlling the occurrence and spread of crop blight caused by *Phytophthora*.

This invention discloses a comprehensive identification method for *Peronobacter spp.* strain QH, belonging to the field of plant pathogen identification technology. The method includes the following steps: obtaining a sample of the *Peronobacter spp.* strain to be identified; extracting morphological characteristics of hyphae, sporangia, and conidiophores; extracting key morphological parameters and generating a morphological feature vector; amplifying its housekeeping gene fragment using polymerase chain reaction and sequencing to obtain the nucleotide sequence; calculating sequence similarity and generating a molecular feature vector; performing comprehensive matching and judgment, and outputting the identification result; generating an identification report, and combining it with strain information in a database for source tracing analysis and risk warning. This invention solves the problems of traditional identification methods relying on single indicators, strong subjectivity, and difficulty in distinguishing intraspecific genetic variations by multimodal fusion of morphological quantitative characteristics and molecular biological marker characteristics. It achieves accurate, objective, and automated identification of *QH* strains, providing data support for disease source tracing and control decisions.

The application discloses a method for reversibly inhibiting the maturation of a Porphyra haitanensis filament, and belongs to the technical field of Porphyra haitanensis breeding. The method comprises adding DMTU to a filament culture medium, and provides a method for inhibiting the maturation of a Porphyra haitanensis filament. When maturation is started, the filament culture solution containing DMTU is used to promote the maturation of the filament, so that the growth of the Porphyra haitanensis filament and the formation of sporangial branches can be effectively inhibited. The inhibiting effect is reversible, and normal maturation can be restored after the treatment is stopped. The inhibiting effect is immediately released, and then the precise regulation of the maturation process of the filament is realized, and the large number of "miscarriages" of chlamydospores is effectively prevented. Therefore, the maturation inhibition method has a good application prospect, and has important practical significance and application value for improving the technical level of the Porphyra haitanensis breeding industry and guaranteeing the sustainable development of the industry.