42 results about "Sporangium" patented technology

The invention discloses an ADF protein from phytophthora as well as a coding gene and application thereof. According to the present invention, the PsADF protein provided by the present invention is the protein represented by sequences 2 and 5 in Phytophthora sojae (Phytophthora sojae); the coding gene is shown as a sequence 3 and a sequence 4. Tests prove that the protein provided by the invention plays an important role in the growth and development process of phytophthora sojae, specifically, after the 64-site mutation of the key amino acid functional site of the protein, the sporangium abnormality and zoospore quantity of the phytophthora sojae are reduced, and the pathogenicity is reduced. The protein provided by the invention has very high application value in control of epidemic disease occurrence and epidemic caused by phytophthora, and provides a molecular target for research and development of novel bactericides in the future. The corresponding ADF inhibitors have different degrees of activity on typical plant pathogenic oomycetes such as phytophthora sojae, phytophthora capsici and phytophthora infestans. The protein provided by the invention has great application potential in the aspect of preventing and controlling the crop epidemic diseases caused by phytophthora plant pathogenic oomycetes, and a novel bactericide developed based on the protein as an action target has important practical significance in controlling the occurrence and prevalence of the crop epidemic diseases caused by phytophthora.

The invention belongs to the technical field of modern agriculture, and particularly relates to application of demethylzeylasteral (DZL) to preparation of a product for preventing and treating phytophthora. The invention determines the effect of pentacyclic triterpenoid demethylzeylasteral on activity inhibition of phytophthora capsici and other crop phytophthora, and demethylzeylasteral can inhibit phytophthora, sporangium release, zoospore germination and pathogenicity, which indicates that demethylzeylasteral (DZL) has the effect of resisting phytophthora capsici. Therefore, the compound provided by the invention can be used for preparing a small-molecule inhibitor for growth, development and pathopoiesis of phytophthora of crops, is expected to become a potential drug for resisting phytophthora of crops, and has good development and application values.

The invention provides application of plasmodiophora brassicae infected early-stage chitin in prevention and control of cruciferae clubroot, and relates to a technology and application for preventing and controlling the cruciferae clubroot based on blocking synthesis of zoosporangium chitin in plasmodiophora brassicae infected early-stage chitin. Experiments prove that chitin components exist in zoosporangia in the early stage of plasmodiophora brassicae infection and are synthesized by plasmodiophora brassicae chitin synthetase genes PbCHS3 and PbCHS7, and a new target is provided for prevention and control of clubroot of cruciferae crops. The clubroot can be efficiently prevented and controlled by expressing PbCHS3 and PbCHS7 silencing vectors in arabidopsis thaliana and oilseed rape and treating or applying pesticide polyoxin capable of blocking chitin synthesis through exogenous small RNA, and a key technology and a key method are provided for preventing and controlling the clubroot of cruciferae.

The invention discloses a method for rapidly acquiring spore seedlings of meniscus violaceum, and relates to the field of asexual reproduction and breeding of laver. The method comprises the following steps: crushing laver to different degrees by using a wall breaking machine, crushing thalli into fragments with certain sizes, collecting the fragments, culturing the fragments for several days, and adding seedling ropes after the fragments develop into monosporangiums and start to diffuse monospores. The seedling ropes and the fragments are mixed and subjected to aerobic culture, and a large number of monospore seedlings can be obtained. According to the mechanical crushing method, a manual chopping crushing method can be replaced, the crushing efficiency of the laver is greatly improved, and large-scale induction and diffusion of the purple menu spores can be realized. Meanwhile, by conducting joint aerobic culture on the fragments and the seedling ropes, the step of collecting the monospore seedlings can be simplified, a large number of monospores are effectively attached to the seedling ropes and rapidly develop into the monospore seedlings, and large-scale monospore seedling culture becomes possible.

The application discloses application of 5,8,11-eicosatrienoic acid in prevention and treatment of plant fungal diseases and belongs to the field of pesticide chemistry. The 5,8,11-eicosatrienoic acid mainly prevents and treats diseases caused by the oomycete class capsicum phytophthora, the compound is a long-chain unsaturated fatty acid, has an antifungal disease effect and can be used as a fungicide to prevent and treat plant diseases. The compound can effectively prevent pathogenic bacteria from invading plants by inhibiting mycelium growth, enhancing cell membrane permeability and interfering with the spore sac release process, and is further used for preventing and treating huge plant fungal diseases. Biological tests show that the compound has a significant concentration-dependent inhibitory effect on the mycelium growth of the capsicum phytophthora, and the half effective inhibitory concentration (EC50) is 60.2 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is more than 83%, the 5,8,11-eicosatrienoic acid can effectively inhibit the release of the capsicum phytophthora spore sac, and the EC50 is 156.3 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is morethan 77%.

The invention provides a streptomyces flavogriseus C-21 strain, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the registration number of the streptomyces flavogriseus C-21 strain is CGMCC No. 36050. The preparation method of the sterile fermentation filtrate of the streptomyces fulvus C-21 comprises the following steps: inoculating a strain C-21 to an oat culture medium plate in a streak manner, culturing for 7 days at 28 DEG C, scraping spores on the surface of the culture medium, adding sterile water to prepare a spore suspension with the concentration of 108 / mL, inoculating the spore suspension into a millet immersion culture solution according to the inoculum size of 2%, and culturing for 7 days at the temperature of 28 DEG C to obtain the sterile fermentation filtrate of the streptomyces fulvus C-21. Performing shaking culture for 7 days in a constant-temperature shaking table at the temperature of 28 DEG C and the speed of 180 r / min, centrifuging for 30 minutes at the speed of 12000 r / min, and filtering supernate by using a 0.22 mu m microporous filter membrane to obtain sterile fermentation filtrate. The streptomyces fulvus C-21 can generate extracellular enzymes such as cellulase, protease and amylase, sterile fermentation filtrate of the streptomyces fulvus C-21 can destroy mycelial morphology and cell membrane integrity of the tomato late blight bacteria, mycelial growth, sporangium germination and zoospore release of the tomato late blight bacteria are remarkably inhibited, and the streptomyces fulvus C-21 has a good control effect on the tomato late blight.

The application provides a kelp breeding performance evaluation method based on sporangium spore dissemination parameters and application, and belongs to the technical field of seaweed cultivation. The method comprises the following steps: S1, taking mature kelp samples, washing, and air-drying treatment to obtain pretreated kelp; S2, taking kelp tissue blocks from the pretreated kelp obtained in step S1, loading the kelp tissue blocks into a mesh bag, immersing the kelp tissue blocks in seawater, promoting the kelp tissue blocks to disseminate, and collecting zoospore liquid; S3, measuring sporangium spore dissemination parameters in step S2; S4, calculating dissemination capacity according to the sporangium spore dissemination parameters obtained in step S3, and evaluating the breeding performance of the kelp according to the dissemination capacity. The above method can quantify the sporangium spore dissemination characteristics of kelp, systematically evaluate the breeding potential of kelp, and improve the breeding efficiency and yield of kelp.

The invention belongs to the field of plant spore collection, and provides a plant spore collecting device which comprises a mounting shell, a protective shell arranged on the upper side of the mounting shell, a connecting pipe fixedly communicated with the upper side of the protective shell, a siphon fixedly communicated with the upper end of the connecting pipe, a funnel-shaped mounting shell and a supporting ring arranged in the mounting shell. Placing boxes are placed in the supporting rings, through holes are formed in the lower surface of the mounting shell, and the three supporting rings are evenly distributed in the mounting shell; by arranging a combined structure of the funnel-shaped mounting shell and the protective shell and cooperating with the design of a siphon and a drainage block, air flow in the device is effectively promoted, drying and breaking of sporangiums are accelerated, and therefore the spore collecting efficiency is improved, a gap can be reserved between the containing box and the inner wall of the mounting shell through an arranged supporting ring, and the sporangiums are prevented from being damaged. And meanwhile, spores and the like can be placed in the placement box, so that the possibility of pollution to the interior of the mounting shell is reduced.

The application concerns a microbiological system supporting the consolidation of sand and sandy soils through biocementation, utilizing a mixture of bacteria from the Sporosarcina genus, including Sporosarcina sp. ANT_H38, deposited in the Polish Collection of Microorganisms (PCM) at the Institute of Immunology and Experimental Therapy of the Polish Academy of Sciences in Wrocław under number B / 00547, and Sporosarcina pasteurii DSM 33. The goal is to stabilize and strengthen soil structures through natural mineralization, which increases the substrate's load-bearing capacity. The process involves culturing the bacteria on LB medium, diluting them and combining them with urea, and then applying them to sand or soil with the addition of a cementing medium. The system also operates effectively at low temperatures, enabling broad applications in construction and environmental protection. The application also concerns a method for producing the microbiological system and a method for supporting the consolidation process of sand and / or sandy soils using this system.

The application provides a kind of double (2,3-dihydroxy benzamide) compound and its preparation method and application, belong to medical technology field.The application obtains a series of double (2,3-dihydroxy benzamide) compound with novel structure and good inhibiting effect on cholestatic liver disease and liver fibrosis by structural modification of sporangium chelae, the compound can significantly inhibit the expression of hepatic stellate cell COL1A1 mRNA in vitro, has strong anti-liver fibrosis effect;In vivo, it can significantly alleviate the liver injury related biochemical indicators of 3,5-diethoxycarbonyl-1,4-dihydro-2,4,6-trimethylpyridine (DDC) induced cholestatic liver disease mouse model, inhibit the expression of liver fibrosis related genes, reduce the content of liver collagen fiber, inhibit liver inflammation and bile duct response, and can be used for treating cholestatic liver disease and liver fibrosis.

The invention provides application of rhizopus microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus It is found for the first time that the rhizopus microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Moreover, the strain has the capabilities of producing cellulase, fixing nitrogen, decomposing inorganic phosphorus and producing siderophores, and pot experiments show that the strain can promote the growth of peppers. The invention provides a new way for biological prevention and control of pepper phytophthora blight, and has positive significance for reducing the use of pesticides and improving the yield and quality of pepper.

The invention provides a monoclonal antibody for specifically recognizing cysts and oocysts / sporangiums of sheep sarcocysts and application of the monoclonal antibody, and the monoclonal antibody provided by the invention can generate positive reaction aiming at soluble antigens of adult holoproteins of sarcocysts tenella and soluble antigens of oocysts / sporangiums of the sarcocysts tenella. The two key development stages of adults and oocysts / sporangiums are covered at the same time. A complete parasite life cycle detection system can be established, the clinical sample detection rate is remarkably improved (experiment shows that the clinical sample detection rate is improved by 35-40%), and the detection window period is 7-10 days earlier than that of a traditional method. Meanwhile, the ELISA detection kit and the detection method thereof provided by the invention are high in sensitivity and convenient to operate, can greatly improve the detection efficiency, and have a good industrial application prospect.

This invention discloses an oligosaccharide syltransferase STT3 subunit from *Phytophthora sojae*, its encoding gene, and its applications. The protein sequence of the oligosaccharide syltransferase STT3 subunit provided by this invention is shown in Sequence 2; its encoding gene is shown in Sequence 1. Experiments have demonstrated that the protein provided by this invention plays an important role in the growth and development of *Phytophthora sojae*, specifically manifested in the slowed mycelial growth, reduced number of sporangia and zoospores, and decreased pathogenicity of *Phytophthora sojae* after its deletion. These conclusions provide a technical basis for exploring the molecular mechanisms of *Phytophthora sojae* development and pathogenicity, and provide molecular targets for the future development of novel fungicides.

The invention relates to a kelp development stage-CIELAB parameter standard database and a method for regulating and monitoring the maturity of kelp sporangium by using the same, and belongs to the technical field of large seaweed cultivation and seedling raising. The method comprises the following steps: firstly, establishing a development stage-CIELAB parameter standard database, and then measuring the CIELAB value of a sample to determine the maturity of kelp sporangium; the development stage of a sample is judged according to the established standard database, and the development process of kelp is regulated and controlled by using light quality according to production requirements, so that the requirements of kelp seedlings at different time can be met, the season dependence bottleneck of traditional seedling culture is solved, and cross-season and multi-batch efficient production of winter seedlings and the like becomes possible.

The invention discloses a method for selecting and ripening sea urchin seed galls, and relates to the field of seed breeding of marine treasures in the marine aquaculture industry. The method for selecting and ripening sea urchin seed galls comprises the following steps: before the sea urchins are naturally bred in the sea area, sea urchins weighing 40-60g are collected, transferred to a ripening pool, aerated and fed in sand-filtered seawater for cultivation, and after 5-6 months of feeding, when the temperature of the sand-filtered seawater drops to 8°C, the sea urchins are fed at a constant temperature for 5-10 days, and then the temperature is raised for ripening. When the water temperature for ripening the seed galls is greater than 19°C, fresh kelp, fresh wakame sporangium leaves, and cooked purple mussel meat are fed in turn, and when the accumulated temperature for ripening is ≥2500°C and the gonad index is ≥20%, the sea urchin seed galls that can be used for inducing production are obtained. The method for selecting and ripening sea urchin seed galls of the invention can greatly reduce its mortality rate, improve the development effect, and quickly meet the inducing production requirements of spring seedling production.

The utility model discloses a downy mildew spore collecting device which comprises a holding assembly, a collecting assembly, a filtering assembly, a catching assembly and a suction assembly, and the holding assembly is sequentially provided with a filtering bin, a catching bin, an air pump bin and a holding part from front to back; the filtering assembly is installed in the filtering bin, the capturing assembly is installed in the capturing bin, the suction assembly is arranged in the air pump bin, the collecting assembly is installed in front of the filtering assembly, and the suction assembly is electrically connected with the power source. According to the device, the holding assembly, the collecting assembly, the filtering assembly, the capturing assembly and the suction assembly are integrated, so that efficient collection of downy mildew spores is achieved. Due to the innovative structural design, the collecting process is quicker and more convenient, and the collecting efficiency is remarkably improved. According to the device, the combination of the filtering assembly and the capturing assembly is adopted, the collection concentration of sporangium is effectively improved, an additional centrifugal concentration step is not needed, the operation process is simplified, and time and cost are saved.

The invention discloses a glucosidase protein (GLS1) from phytophthora plant pathogenic oomycetes as well as a coding gene and application thereof. The PsGLS1 protein provided by the invention is a protein of which the sequence is as shown in SEQ ID No. 1 in Phytophthora sojae, and the PsGLS1 protein is a protein of which the sequence is as shown in SEQ ID No. 2 in Phytophthora sojae; the coding gene of the gene is shown as SEQ ID No.2. Experiments prove that the protein provided by the invention plays an important role in the growth and development process of phytophthora sojae, and after the PsGLS1 gene is knocked out, the mycelial growth rate, the sporangium number, the zoospore yield and the resting spore germination rate of the phytophthora sojae are obviously reduced, and the pathogenicity of pathogenic bacteria is obviously influenced. Glucosidase inhibitor mispermine has an inhibition effect on the growth of phytophthora sojae hyphae. In conclusion, the PsGLS1 gene has very high application value in control of epidemic disease occurrence and epidemic caused by phytophthora, and a molecular target is provided for research and development of novel bactericides in the future.

The invention provides a method for detecting the activity of coccidian oocysts and judging the quality grade of the coccidian oocysts, and the method for detecting the activity of the coccidian oocysts comprises the following steps: obtaining the sporozoite escape rate M of a to-be-detected coccidian oocyst sample, and determining whether the activity of the coccidian oocysts is qualified or not according to the sporozoite escape rate M, the method for obtaining the sporozoite escape rate M comprises the following steps: (1) preparing a coccidian oocyst suspension of a coccidian oocyst sample; (2) carrying out wall breaking on oocysts in the coccidium oocyst suspension to enable the oocysts to release sporangiums so as to obtain a sporangium suspension; (3) digesting the obtained sporangium suspension by using digestive juice, so that sporangium releases sporozoites to obtain a sporozoite suspension, counting the sporozoites and sporangium in the sporozoite suspension, and marking the number of the sporozoites and sporangium as B and C respectively, and (4) calculating the sporozoite escape rate M: when the sporozoite escape rate M is greater than or equal to 70%, determining that the activity of the coccidium oocysts is qualified.

This invention discloses a glucosidase protein (GLS1) from *Phytophthora*, a plant pathogenic oomycete, its encoding gene, and its applications. The PsGLS1 protein provided by this invention is effective against *Phytophthora soybean* (…). Phytophthora sojae The protein in this invention is shown in SEQ ID No. 1; its encoding gene is shown in SEQ ID No. 2. Experiments have demonstrated that the protein provided by this invention plays an important role in the growth and development of *Phytophthora soybeani*. PsGLS1 Gene knockout significantly reduced the mycelial growth rate, sporangium number, zoospore yield, and resting spore germination rate of *Phytophthora soybeani*, and also significantly affected the pathogenicity of the fungus. The glucosidase inhibitor sericite also inhibited the mycelial growth of *Phytophthora soybeani*. In summary, this invention… PsGLS1 Genes have high application value in controlling the occurrence and spread of diseases caused by Phytophthora, and provide molecular targets for the development of new fungicides in the future.

The invention discloses a tissue culture method for botrychium ternatum spore induced prothallium. The culture method comprises the following steps: collecting botrychium ternatum with sporangium, drying, sieving and collecting spores, sequentially carrying out multiple times of disinfection and cleaning pretreatment on the collected spores by using 75% ethanol, sterile water, 2% sodium hypochlorite and the like in a sterile environment, uniformly coating a culture medium with treated spore suspension, and carrying out observation culture, the time from spore inoculation to prothallium formation is 24-26 days, and the time from spore inoculation to juvenile sporophyte formation is 47-63 days. The culture medium consists of the following components: a 1 / 8 MS culture medium, 5g / L of sucrose and 8g / L of agar. The culture medium is simple to prepare, can effectively induce spore germination and prothallus proliferation of the botrychium ternatum, can be further cultured to obtain sporophytes, can also be used for acclimatization and transplantation, shortens the seedling raising period, further realizes large-scale cultivation of the botrychium ternatum, and has important significance for expanded application of pteridophyte.

The invention provides a kelp breeding performance evaluation method based on sporangium spore diffusion parameters and application, and belongs to the technical field of seaweed cultivation. The method comprises the following steps: S1, taking a mature kelp sample, cleaning, and drying in the shade to obtain pretreated kelp; s2, taking kelp tissue blocks from the pretreated kelp obtained in the step S1, putting the kelp tissue blocks into a mesh bag, immersing the mesh bag into seawater to promote diffusion, and collecting zoospores liquid; s3, determining diffusion parameters of the sporangium spores in the step S2; and S4, calculating the diffusion capacity according to the diffusion parameters of the sporangium spores obtained in the step S3, and evaluating the breeding performance of the kelp according to the diffusion capacity. By utilizing the method to quantify the diffusion characteristics of the sporangium spores of the kelp, the breeding potential of the kelp can be systematically evaluated, and the breeding efficiency and yield of the kelp are improved.

The invention provides a method for preserving germplasm resources of adiantum obtusifolium, which comprises the following steps: step 1, collecting uncracked sporangiums on sporophytes of adiantum obtusifolium, treating the sporangiums with 0.05-0.1% mercuric chloride, then cleaning the sporangiums, and inoculating the sporangiums to a culture medium; 2, transferring the sporangium on the culture medium to a germination culture medium after the sporangium passes through a pollution period, culturing until the sporangium germinates to form prothalli, then transferring the prothalli to a proliferation culture medium, and culturing and proliferating to form proliferated prothalli; 3, prothalli needing to be preserved are selected from the proliferated prothalli and transferred to a preservation culture medium to be preserved and cultured, and preserved adiantum obtusifolium is obtained; the preservation culture medium is a 1 / 8 MS culture medium containing 0.03 to 0.05 mg / L of 6-BA, 0.1 to 0.4 mg / L of NAA, 1.5 to 3.0 mg / L of IBA and 0.2 to 0.4 mg / L of CCC.

The invention discloses six kinds of microRNAs (microRNA, miRNA), namely miR2, miR3, miR8, miR53, miR117 and miR157 capable of regulating and controlling the development and pathopoiesis of Phytophthora capsici, and application of the six kinds of microRNAs, namely miR2, miR3, miR8, miR53, miR117 and miR157. The miRNAs have a ribonucleic acid sequence as shown in any of SEQ ID NO.1-6. The invention also discloses a preparation method of the miRNAs. The miRNAs involved in the invention can participate in the development and pathopoiesis of the phytophthora capsici, after the expression of the miRNAs is influenced, the capability of producing sporangium and zoospore of the phytophthora capsici is greatly reduced, the pathopoiesis capability is almost lost, and the miRNAs conservatively exist in different phytophthora capsici. The gene provided by the invention has a very high application value in preventing and treating crop epidemic diseases caused by phytophthora pathogenic bacteria such as phytophthora capsici, and the miRNAs are taken as targets to develop nucleic acid pesticides, so that the miRNAs have important significance in controlling the occurrence and prevalence of the crop epidemic diseases.

The present invention relates to etoxazole as a fungal and oomycete chitin synthase inhibitor and its novel application in sterilization. In vitro enzyme activity inhibition tests and sterilization tests demonstrate that etoxazole can significantly inhibit the activity of fungal and oomycete chitin synthases, and can also inhibit the growth of fungal hyphae, the production of oomycete sporangia, and the toxicity of infection. Therefore, etoxazole can be used as a bactericide for the prevention and treatment of fungal and oomycete diseases.

The application provides application of chitin in early infection of plasmodiophora brassicae in prevention and control of brassica root tumour disease, and is based on the technology and application of blocking chitin synthesis of zoosporangium in early infection of plasmodiophora brassicae to prevent and control brassica root tumour disease. It is proved by experiments that the zoosporangium in early infection of plasmodiophora brassicae contains chitin, and the chitin is synthesized by plasmodiophora brassicae chitin synthase genes PbCHS3 and PbCHS7, thereby providing a new target for prevention and control of brassica root tumour disease. Through expression of PbCHS3 and PbCHS7 silencing vectors, exogenous small RNA treatment or application of polyoxin, which can block chitin synthesis, in arabidopsis and rape, brassica root tumour disease can be prevented and controlled efficiently, and key technology and method for prevention and control of brassica root tumour disease are provided.

The present invention provides the application of daidzin in preventing and controlling Phytophthora capsici. Daidzin can effectively inhibit the vegetative growth of Phytophthora capsici, and is expected to be used for preparing a new type of fungicide against Phytophthora capsici, reducing pesticide resistance and improving the control effect on Phytophthora capsici. In the application of daidzin in preventing and controlling Phytophthora capsici provided by the present invention, it has a certain inhibitory effect on three types of spores produced by Phytophthora capsici. Daidzin at a concentration of 1 μM - 5 μM can induce apoptotic-like death of the spores of Phytophthora capsici, and daidzin at a concentration of 7 μM - 15 μM can inhibit the release of sporangia of Phytophthora capsici and effectively inhibit the germination of zoospores of Phytophthora capsici. Using daidzin also helps to improve the disease resistance of susceptible varieties to Phytophthora capsici, which is simple, effective, green and environmentally friendly.

This invention belongs to the field of modern agricultural technology and specifically relates to the use of demethylzeylasteral (DZL) in the preparation of products for controlling Phytophthora spp. This invention demonstrates the inhibitory effect of pentacyclic triterpenoid demethylzeylasteral on the activity of Phytophthora capsici and other crop fungi. Demethylzeylasteral can inhibit the fungus, sporangium release, zoospore germination, and pathogenicity, demonstrating its anti-Phytophthora spp. activity. Therefore, the compounds provided by this invention can be used to prepare small molecule inhibitors of the growth, development, and pathogenicity of Phytophthora spp., demonstrating their potential as potential anti-Phytophthora spp. drugs and possessing promising development and application value.

The present application relates to the field of microbial culture, in particular to a culture medium preparation and culture method for inducing the germination of blood ear spores into mycelium. The culture medium ratio comprises 500-700 mL of 200 g potato extract, 300-500 mL of blood mark rigid leather fungus fermentation supernatant, 20 g of glucose, 20 g of agar, 0.46 g of potassium dihydrogen phosphate, 1.0 g of calcium superphosphate, 0.5 g of magnesium sulfate, 2 g of fish peptone, 20 g of soybean hull, and 1 mg of VB6. The blood ear spores are inoculated into the culture medium, and cultured in a constant temperature incubator at 20-23.5 DEG C. Mycelium can be observed around the blood ear spores after 15-20 days. Therefore, the stable germination of blood ear spores into mycelium is successfully induced, and the problem of obtaining pure mycelium of blood ear is solved. From the aspect of cultivation, the difficulty of strain preparation in artificial cultivation of blood ear is reduced. From the aspect of research, the implementation of the present application has great significance for the related basic biological research of blood ear.

The present invention relates to novel applications of thiazolinone as a fungal and oomycete chitin synthase inhibitor and its sterilization application. In vitro enzyme activity inhibition tests and sterilization tests demonstrate that thiazolinone can significantly inhibit the activity of fungal and oomycete chitin synthases, inhibit fungal spore production and hyphal growth, and inhibit the production and infection toxicity of oomycete sporangia. The invention can be used as a sterilant for the prevention and treatment of fungal and oomycete diseases.

The invention relates to the technical field of marine biotechnology and ecological restoration, in particular to coral source calcified algae Z1 and a culture method and application thereof. The invention discovers and identifies a new coral source calcified algae species, namely calcified algae Z1 (Acrochaete zhaohaiensis sp.nov Z1), and the preservation number of the calcified algae Z1 is CCTCC (China Center for Type Culture Collection) NO: M 2025578. The surface of a calcified algae Z1 cell has a unique nanoscale calcium carbonate needle-like protrusion and hexagonal lattice arrangement structure, the strain and known coral symbiotic algae form an independent branch on a phylogenetic tree, the calcification rate of the calcified algae Z1 is obviously improved compared with that of typical zooxanthellae, a complete calcified tissue can be formed within 20 days, the calcification rate is high, and the calcification effect is good. Potential technical reserve is provided for follow-up symbiotic relationship research and artificial reef body construction by the reproduction characteristics of the static sporangiums. Meanwhile, the unique calcification metabolism characteristic provides a novel functional material for coral reef repair.