33 results about "Sporangium" patented technology

The invention belongs to the technical field of modern agriculture, and particularly relates to application of demethylzeylasteral (DZL) to preparation of a product for preventing and treating phytophthora. The invention determines the effect of pentacyclic triterpenoid demethylzeylasteral on activity inhibition of phytophthora capsici and other crop phytophthora, and demethylzeylasteral can inhibit phytophthora, sporangium release, zoospore germination and pathogenicity, which indicates that demethylzeylasteral (DZL) has the effect of resisting phytophthora capsici. Therefore, the compound provided by the invention can be used for preparing a small-molecule inhibitor for growth, development and pathopoiesis of phytophthora of crops, is expected to become a potential drug for resisting phytophthora of crops, and has good development and application values.

The application discloses application of 5,8,11-eicosatrienoic acid in prevention and treatment of plant fungal diseases and belongs to the field of pesticide chemistry. The 5,8,11-eicosatrienoic acid mainly prevents and treats diseases caused by the oomycete class capsicum phytophthora, the compound is a long-chain unsaturated fatty acid, has an antifungal disease effect and can be used as a fungicide to prevent and treat plant diseases. The compound can effectively prevent pathogenic bacteria from invading plants by inhibiting mycelium growth, enhancing cell membrane permeability and interfering with the spore sac release process, and is further used for preventing and treating huge plant fungal diseases. Biological tests show that the compound has a significant concentration-dependent inhibitory effect on the mycelium growth of the capsicum phytophthora, and the half effective inhibitory concentration (EC50) is 60.2 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is more than 83%, the 5,8,11-eicosatrienoic acid can effectively inhibit the release of the capsicum phytophthora spore sac, and the EC50 is 156.3 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is morethan 77%.

The invention provides a streptomyces flavogriseus C-21 strain, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the registration number of the streptomyces flavogriseus C-21 strain is CGMCC No. 36050. The preparation method of the sterile fermentation filtrate of the streptomyces fulvus C-21 comprises the following steps: inoculating a strain C-21 to an oat culture medium plate in a streak manner, culturing for 7 days at 28 DEG C, scraping spores on the surface of the culture medium, adding sterile water to prepare a spore suspension with the concentration of 108 / mL, inoculating the spore suspension into a millet immersion culture solution according to the inoculum size of 2%, and culturing for 7 days at the temperature of 28 DEG C to obtain the sterile fermentation filtrate of the streptomyces fulvus C-21. Performing shaking culture for 7 days in a constant-temperature shaking table at the temperature of 28 DEG C and the speed of 180 r / min, centrifuging for 30 minutes at the speed of 12000 r / min, and filtering supernate by using a 0.22 mu m microporous filter membrane to obtain sterile fermentation filtrate. The streptomyces fulvus C-21 can generate extracellular enzymes such as cellulase, protease and amylase, sterile fermentation filtrate of the streptomyces fulvus C-21 can destroy mycelial morphology and cell membrane integrity of the tomato late blight bacteria, mycelial growth, sporangium germination and zoospore release of the tomato late blight bacteria are remarkably inhibited, and the streptomyces fulvus C-21 has a good control effect on the tomato late blight.

The application provides a kelp breeding performance evaluation method based on sporangium spore dissemination parameters and application, and belongs to the technical field of seaweed cultivation. The method comprises the following steps: S1, taking mature kelp samples, washing, and air-drying treatment to obtain pretreated kelp; S2, taking kelp tissue blocks from the pretreated kelp obtained in step S1, loading the kelp tissue blocks into a mesh bag, immersing the kelp tissue blocks in seawater, promoting the kelp tissue blocks to disseminate, and collecting zoospore liquid; S3, measuring sporangium spore dissemination parameters in step S2; S4, calculating dissemination capacity according to the sporangium spore dissemination parameters obtained in step S3, and evaluating the breeding performance of the kelp according to the dissemination capacity. The above method can quantify the sporangium spore dissemination characteristics of kelp, systematically evaluate the breeding potential of kelp, and improve the breeding efficiency and yield of kelp.

The invention belongs to the field of plant spore collection, and provides a plant spore collecting device which comprises a mounting shell, a protective shell arranged on the upper side of the mounting shell, a connecting pipe fixedly communicated with the upper side of the protective shell, a siphon fixedly communicated with the upper end of the connecting pipe, a funnel-shaped mounting shell and a supporting ring arranged in the mounting shell. Placing boxes are placed in the supporting rings, through holes are formed in the lower surface of the mounting shell, and the three supporting rings are evenly distributed in the mounting shell; by arranging a combined structure of the funnel-shaped mounting shell and the protective shell and cooperating with the design of a siphon and a drainage block, air flow in the device is effectively promoted, drying and breaking of sporangiums are accelerated, and therefore the spore collecting efficiency is improved, a gap can be reserved between the containing box and the inner wall of the mounting shell through an arranged supporting ring, and the sporangiums are prevented from being damaged. And meanwhile, spores and the like can be placed in the placement box, so that the possibility of pollution to the interior of the mounting shell is reduced.

The application concerns a microbiological system supporting the consolidation of sand and sandy soils through biocementation, utilizing a mixture of bacteria from the Sporosarcina genus, including Sporosarcina sp. ANT_H38, deposited in the Polish Collection of Microorganisms (PCM) at the Institute of Immunology and Experimental Therapy of the Polish Academy of Sciences in Wrocław under number B / 00547, and Sporosarcina pasteurii DSM 33. The goal is to stabilize and strengthen soil structures through natural mineralization, which increases the substrate's load-bearing capacity. The process involves culturing the bacteria on LB medium, diluting them and combining them with urea, and then applying them to sand or soil with the addition of a cementing medium. The system also operates effectively at low temperatures, enabling broad applications in construction and environmental protection. The application also concerns a method for producing the microbiological system and a method for supporting the consolidation process of sand and / or sandy soils using this system.

The application provides a kind of double (2,3-dihydroxy benzamide) compound and its preparation method and application, belong to medical technology field.The application obtains a series of double (2,3-dihydroxy benzamide) compound with novel structure and good inhibiting effect on cholestatic liver disease and liver fibrosis by structural modification of sporangium chelae, the compound can significantly inhibit the expression of hepatic stellate cell COL1A1 mRNA in vitro, has strong anti-liver fibrosis effect;In vivo, it can significantly alleviate the liver injury related biochemical indicators of 3,5-diethoxycarbonyl-1,4-dihydro-2,4,6-trimethylpyridine (DDC) induced cholestatic liver disease mouse model, inhibit the expression of liver fibrosis related genes, reduce the content of liver collagen fiber, inhibit liver inflammation and bile duct response, and can be used for treating cholestatic liver disease and liver fibrosis.

The invention provides application of rhizopus microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus It is found for the first time that the rhizopus microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Moreover, the strain has the capabilities of producing cellulase, fixing nitrogen, decomposing inorganic phosphorus and producing siderophores, and pot experiments show that the strain can promote the growth of peppers. The invention provides a new way for biological prevention and control of pepper phytophthora blight, and has positive significance for reducing the use of pesticides and improving the yield and quality of pepper.

The invention provides a monoclonal antibody for specifically recognizing cysts and oocysts / sporangiums of sheep sarcocysts and application of the monoclonal antibody, and the monoclonal antibody provided by the invention can generate positive reaction aiming at soluble antigens of adult holoproteins of sarcocysts tenella and soluble antigens of oocysts / sporangiums of the sarcocysts tenella. The two key development stages of adults and oocysts / sporangiums are covered at the same time. A complete parasite life cycle detection system can be established, the clinical sample detection rate is remarkably improved (experiment shows that the clinical sample detection rate is improved by 35-40%), and the detection window period is 7-10 days earlier than that of a traditional method. Meanwhile, the ELISA detection kit and the detection method thereof provided by the invention are high in sensitivity and convenient to operate, can greatly improve the detection efficiency, and have a good industrial application prospect.

This invention discloses an oligosaccharide syltransferase STT3 subunit from *Phytophthora sojae*, its encoding gene, and its applications. The protein sequence of the oligosaccharide syltransferase STT3 subunit provided by this invention is shown in Sequence 2; its encoding gene is shown in Sequence 1. Experiments have demonstrated that the protein provided by this invention plays an important role in the growth and development of *Phytophthora sojae*, specifically manifested in the slowed mycelial growth, reduced number of sporangia and zoospores, and decreased pathogenicity of *Phytophthora sojae* after its deletion. These conclusions provide a technical basis for exploring the molecular mechanisms of *Phytophthora sojae* development and pathogenicity, and provide molecular targets for the future development of novel fungicides.

The invention relates to a kelp development stage-CIELAB parameter standard database and a method for regulating and monitoring the maturity of kelp sporangium by using the same, and belongs to the technical field of large seaweed cultivation and seedling raising. The method comprises the following steps: firstly, establishing a development stage-CIELAB parameter standard database, and then measuring the CIELAB value of a sample to determine the maturity of kelp sporangium; the development stage of a sample is judged according to the established standard database, and the development process of kelp is regulated and controlled by using light quality according to production requirements, so that the requirements of kelp seedlings at different time can be met, the season dependence bottleneck of traditional seedling culture is solved, and cross-season and multi-batch efficient production of winter seedlings and the like becomes possible.

The utility model discloses a downy mildew spore collecting device which comprises a holding assembly, a collecting assembly, a filtering assembly, a catching assembly and a suction assembly, and the holding assembly is sequentially provided with a filtering bin, a catching bin, an air pump bin and a holding part from front to back; the filtering assembly is installed in the filtering bin, the capturing assembly is installed in the capturing bin, the suction assembly is arranged in the air pump bin, the collecting assembly is installed in front of the filtering assembly, and the suction assembly is electrically connected with the power source. According to the device, the holding assembly, the collecting assembly, the filtering assembly, the capturing assembly and the suction assembly are integrated, so that efficient collection of downy mildew spores is achieved. Due to the innovative structural design, the collecting process is quicker and more convenient, and the collecting efficiency is remarkably improved. According to the device, the combination of the filtering assembly and the capturing assembly is adopted, the collection concentration of sporangium is effectively improved, an additional centrifugal concentration step is not needed, the operation process is simplified, and time and cost are saved.

The invention discloses a glucosidase protein (GLS1) from phytophthora plant pathogenic oomycetes as well as a coding gene and application thereof. The PsGLS1 protein provided by the invention is a protein of which the sequence is as shown in SEQ ID No. 1 in Phytophthora sojae, and the PsGLS1 protein is a protein of which the sequence is as shown in SEQ ID No. 2 in Phytophthora sojae; the coding gene of the gene is shown as SEQ ID No.2. Experiments prove that the protein provided by the invention plays an important role in the growth and development process of phytophthora sojae, and after the PsGLS1 gene is knocked out, the mycelial growth rate, the sporangium number, the zoospore yield and the resting spore germination rate of the phytophthora sojae are obviously reduced, and the pathogenicity of pathogenic bacteria is obviously influenced. Glucosidase inhibitor mispermine has an inhibition effect on the growth of phytophthora sojae hyphae. In conclusion, the PsGLS1 gene has very high application value in control of epidemic disease occurrence and epidemic caused by phytophthora, and a molecular target is provided for research and development of novel bactericides in the future.

The invention provides a method for detecting the activity of coccidian oocysts and judging the quality grade of the coccidian oocysts, and the method for detecting the activity of the coccidian oocysts comprises the following steps: obtaining the sporozoite escape rate M of a to-be-detected coccidian oocyst sample, and determining whether the activity of the coccidian oocysts is qualified or not according to the sporozoite escape rate M, the method for obtaining the sporozoite escape rate M comprises the following steps: (1) preparing a coccidian oocyst suspension of a coccidian oocyst sample; (2) carrying out wall breaking on oocysts in the coccidium oocyst suspension to enable the oocysts to release sporangiums so as to obtain a sporangium suspension; (3) digesting the obtained sporangium suspension by using digestive juice, so that sporangium releases sporozoites to obtain a sporozoite suspension, counting the sporozoites and sporangium in the sporozoite suspension, and marking the number of the sporozoites and sporangium as B and C respectively, and (4) calculating the sporozoite escape rate M: when the sporozoite escape rate M is greater than or equal to 70%, determining that the activity of the coccidium oocysts is qualified.

This invention discloses a glucosidase protein (GLS1) from *Phytophthora*, a plant pathogenic oomycete, its encoding gene, and its applications. The PsGLS1 protein provided by this invention is effective against *Phytophthora soybean* (…). Phytophthora sojae The protein in this invention is shown in SEQ ID No. 1; its encoding gene is shown in SEQ ID No. 2. Experiments have demonstrated that the protein provided by this invention plays an important role in the growth and development of *Phytophthora soybeani*. PsGLS1 Gene knockout significantly reduced the mycelial growth rate, sporangium number, zoospore yield, and resting spore germination rate of *Phytophthora soybeani*, and also significantly affected the pathogenicity of the fungus. The glucosidase inhibitor sericite also inhibited the mycelial growth of *Phytophthora soybeani*. In summary, this invention… PsGLS1 Genes have high application value in controlling the occurrence and spread of diseases caused by Phytophthora, and provide molecular targets for the development of new fungicides in the future.

The invention discloses a tissue culture method for botrychium ternatum spore induced prothallium. The culture method comprises the following steps: collecting botrychium ternatum with sporangium, drying, sieving and collecting spores, sequentially carrying out multiple times of disinfection and cleaning pretreatment on the collected spores by using 75% ethanol, sterile water, 2% sodium hypochlorite and the like in a sterile environment, uniformly coating a culture medium with treated spore suspension, and carrying out observation culture, the time from spore inoculation to prothallium formation is 24-26 days, and the time from spore inoculation to juvenile sporophyte formation is 47-63 days. The culture medium consists of the following components: a 1 / 8 MS culture medium, 5g / L of sucrose and 8g / L of agar. The culture medium is simple to prepare, can effectively induce spore germination and prothallus proliferation of the botrychium ternatum, can be further cultured to obtain sporophytes, can also be used for acclimatization and transplantation, shortens the seedling raising period, further realizes large-scale cultivation of the botrychium ternatum, and has important significance for expanded application of pteridophyte.

The invention provides a method for preserving germplasm resources of adiantum obtusifolium, which comprises the following steps: step 1, collecting uncracked sporangiums on sporophytes of adiantum obtusifolium, treating the sporangiums with 0.05-0.1% mercuric chloride, then cleaning the sporangiums, and inoculating the sporangiums to a culture medium; 2, transferring the sporangium on the culture medium to a germination culture medium after the sporangium passes through a pollution period, culturing until the sporangium germinates to form prothalli, then transferring the prothalli to a proliferation culture medium, and culturing and proliferating to form proliferated prothalli; 3, prothalli needing to be preserved are selected from the proliferated prothalli and transferred to a preservation culture medium to be preserved and cultured, and preserved adiantum obtusifolium is obtained; the preservation culture medium is a 1 / 8 MS culture medium containing 0.03 to 0.05 mg / L of 6-BA, 0.1 to 0.4 mg / L of NAA, 1.5 to 3.0 mg / L of IBA and 0.2 to 0.4 mg / L of CCC.

The invention discloses six kinds of microRNAs (microRNA, miRNA), namely miR2, miR3, miR8, miR53, miR117 and miR157 capable of regulating and controlling the development and pathopoiesis of Phytophthora capsici, and application of the six kinds of microRNAs, namely miR2, miR3, miR8, miR53, miR117 and miR157. The miRNAs have a ribonucleic acid sequence as shown in any of SEQ ID NO.1-6. The invention also discloses a preparation method of the miRNAs. The miRNAs involved in the invention can participate in the development and pathopoiesis of the phytophthora capsici, after the expression of the miRNAs is influenced, the capability of producing sporangium and zoospore of the phytophthora capsici is greatly reduced, the pathopoiesis capability is almost lost, and the miRNAs conservatively exist in different phytophthora capsici. The gene provided by the invention has a very high application value in preventing and treating crop epidemic diseases caused by phytophthora pathogenic bacteria such as phytophthora capsici, and the miRNAs are taken as targets to develop nucleic acid pesticides, so that the miRNAs have important significance in controlling the occurrence and prevalence of the crop epidemic diseases.

The present invention relates to etoxazole as a fungal and oomycete chitin synthase inhibitor and its novel application in sterilization. In vitro enzyme activity inhibition tests and sterilization tests demonstrate that etoxazole can significantly inhibit the activity of fungal and oomycete chitin synthases, and can also inhibit the growth of fungal hyphae, the production of oomycete sporangia, and the toxicity of infection. Therefore, etoxazole can be used as a bactericide for the prevention and treatment of fungal and oomycete diseases.

The application provides application of chitin in early infection of plasmodiophora brassicae in prevention and control of brassica root tumour disease, and is based on the technology and application of blocking chitin synthesis of zoosporangium in early infection of plasmodiophora brassicae to prevent and control brassica root tumour disease. It is proved by experiments that the zoosporangium in early infection of plasmodiophora brassicae contains chitin, and the chitin is synthesized by plasmodiophora brassicae chitin synthase genes PbCHS3 and PbCHS7, thereby providing a new target for prevention and control of brassica root tumour disease. Through expression of PbCHS3 and PbCHS7 silencing vectors, exogenous small RNA treatment or application of polyoxin, which can block chitin synthesis, in arabidopsis and rape, brassica root tumour disease can be prevented and controlled efficiently, and key technology and method for prevention and control of brassica root tumour disease are provided.

This invention belongs to the field of modern agricultural technology and specifically relates to the use of demethylzeylasteral (DZL) in the preparation of products for controlling Phytophthora spp. This invention demonstrates the inhibitory effect of pentacyclic triterpenoid demethylzeylasteral on the activity of Phytophthora capsici and other crop fungi. Demethylzeylasteral can inhibit the fungus, sporangium release, zoospore germination, and pathogenicity, demonstrating its anti-Phytophthora spp. activity. Therefore, the compounds provided by this invention can be used to prepare small molecule inhibitors of the growth, development, and pathogenicity of Phytophthora spp., demonstrating their potential as potential anti-Phytophthora spp. drugs and possessing promising development and application value.

The present application relates to the field of microbial culture, in particular to a culture medium preparation and culture method for inducing the germination of blood ear spores into mycelium. The culture medium ratio comprises 500-700 mL of 200 g potato extract, 300-500 mL of blood mark rigid leather fungus fermentation supernatant, 20 g of glucose, 20 g of agar, 0.46 g of potassium dihydrogen phosphate, 1.0 g of calcium superphosphate, 0.5 g of magnesium sulfate, 2 g of fish peptone, 20 g of soybean hull, and 1 mg of VB6. The blood ear spores are inoculated into the culture medium, and cultured in a constant temperature incubator at 20-23.5 DEG C. Mycelium can be observed around the blood ear spores after 15-20 days. Therefore, the stable germination of blood ear spores into mycelium is successfully induced, and the problem of obtaining pure mycelium of blood ear is solved. From the aspect of cultivation, the difficulty of strain preparation in artificial cultivation of blood ear is reduced. From the aspect of research, the implementation of the present application has great significance for the related basic biological research of blood ear.

The present invention relates to novel applications of thiazolinone as a fungal and oomycete chitin synthase inhibitor and its sterilization application. In vitro enzyme activity inhibition tests and sterilization tests demonstrate that thiazolinone can significantly inhibit the activity of fungal and oomycete chitin synthases, inhibit fungal spore production and hyphal growth, and inhibit the production and infection toxicity of oomycete sporangia. The invention can be used as a sterilant for the prevention and treatment of fungal and oomycete diseases.

The application discloses a culture method for propagating Brachythecium populeum, and belongs to the technical field of Brachythecium populeum tissue culture. The application uses sporangium as an explant to propagate Brachythecium populeum, and quickly obtains Brachythecium populeum spores with a high germination rate, establishes a regeneration system of Brachythecium populeum, lays a foundation for large-scale propagation of Brachythecium populeum, shortens the propagation cycle of Brachythecium populeum, and better maintains excellent properties. The application first establishes a method for culturing Brachythecium populeum by using spores, fills the blank of the prior art, and provides a technical basis for propagation, seed preservation, preparation of protoplasts, recombination experiments and gametophyte culture of Brachythecium populeum.

This invention discloses an arginine methyltransferase protein from *Phytophthora* plant pathogenic oomycetes, its encoding gene, and its applications. The arginine methyltransferase protein provided by this invention is shown in sequences 6-8; its encoding gene is shown in sequences 1-3. Experiments have demonstrated that the protein of this invention plays an important role in the growth, development, and pathogenicity of *Phytophthora capsici*, a typical plant pathogenic oomycete. The absence or reduced expression level of this protein leads to slower mycelial growth rate, reduced sporangium production, decreased zoospore numbers, and reduced pathogenicity of *Phytophthora capsici*. Corresponding arginine methyltransferase inhibitors exhibit varying degrees of activity against typical plant pathogenic oomycetes such as *Phytophthora capsici*, *Phytophthora soybeani*, and *Phytophthora pathogenica*. The gene provided by this invention has significant application potential in the prevention and control of crop blight caused by *Phytophthora* plant pathogenic oomycetes. Novel fungicides developed based on this protein as a target have important practical significance for controlling the occurrence and spread of crop blight caused by *Phytophthora*.

This invention discloses a comprehensive identification method for *Peronobacter spp.* strain QH, belonging to the field of plant pathogen identification technology. The method includes the following steps: obtaining a sample of the *Peronobacter spp.* strain to be identified; extracting morphological characteristics of hyphae, sporangia, and conidiophores; extracting key morphological parameters and generating a morphological feature vector; amplifying its housekeeping gene fragment using polymerase chain reaction and sequencing to obtain the nucleotide sequence; calculating sequence similarity and generating a molecular feature vector; performing comprehensive matching and judgment, and outputting the identification result; generating an identification report, and combining it with strain information in a database for source tracing analysis and risk warning. This invention solves the problems of traditional identification methods relying on single indicators, strong subjectivity, and difficulty in distinguishing intraspecific genetic variations by multimodal fusion of morphological quantitative characteristics and molecular biological marker characteristics. It achieves accurate, objective, and automated identification of *QH* strains, providing data support for disease source tracing and control decisions.

The present invention discloses a fluorescence quantitative PCR detection method for the concentration of resting sporangia of potato powdery scab pathogen. In this method, the fluorescence quantitative PCR primer set for detecting the concentration of resting sporangia of potato powdery scab pathogen is composed of the nucleotide shown in Sequence 1 in the sequence listing and the nucleotide shown in Sequence 2 in the sequence listing. The method of the present invention has a fast detection speed, high sensitivity and strong specificity, is more conducive to the detection of resting sporangia of potato powdery scab pathogen in potato seeds and soil, and provides a basis for the prevention of potato powdery scab.

The invention provides a Rosa Vietnamese for promoting porphyra haitanensis filamentous algal filaments to develop into shell sporangia branches, and the provided strain can promote formation of the shell sporangia branches of porphyra haitanensis filaments so as to shorten the breeding cycle of the porphyra haitanensis filaments. The preservation number of the rose Vietnamese provided by the invention is CGMCC (China General Microbiological Culture Collection Center) NO.34643. According to the method, the screened rosa Vietnamese strain bacterial liquid is added into the solution for culturing the protonema, so that the development time of the conchosporangium branches can be effectively shortened, the formation rate of the conchosporangium branches can be increased, and the problems that the formation rate of the conchosporangium branches is insufficient, the formation period is too long and the like are solved.

The invention discloses an FHA1 protein from Phytophthora spp. And an application of the FHA1 protein in screening of a protein-protein interaction (PPI) inhibitor. The amino acid sequence of the FHA1 protein is shown as a sequence 3. The protein can interact with phytophthora sojae 14-3-3 protein, and plays a key role in the production process of pathogenic bacteria sporangium and zoospore. By targeting the interaction between the FHA1 protein and phytophthora 14-3-3 protein, the interaction between the FHA1 protein and phytophthora 14-3-3 protein is inhibited so as to influence the normal life activity of phytophthora sojae, and the FHA1 protein can be used as a bacteriostatic or bactericide target for screening phytophthora bacteriostat or bactericide.