23 results about "Sporangium" patented technology

The application discloses application of 5,8,11-eicosatrienoic acid in prevention and treatment of plant fungal diseases and belongs to the field of pesticide chemistry. The 5,8,11-eicosatrienoic acid mainly prevents and treats diseases caused by the oomycete class capsicum phytophthora, the compound is a long-chain unsaturated fatty acid, has an antifungal disease effect and can be used as a fungicide to prevent and treat plant diseases. The compound can effectively prevent pathogenic bacteria from invading plants by inhibiting mycelium growth, enhancing cell membrane permeability and interfering with the spore sac release process, and is further used for preventing and treating huge plant fungal diseases. Biological tests show that the compound has a significant concentration-dependent inhibitory effect on the mycelium growth of the capsicum phytophthora, and the half effective inhibitory concentration (EC50) is 60.2 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is more than 83%, the 5,8,11-eicosatrienoic acid can effectively inhibit the release of the capsicum phytophthora spore sac, and the EC50 is 156.3 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is morethan 77%.

The invention provides a streptomyces flavogriseus C-21 strain, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the registration number of the streptomyces flavogriseus C-21 strain is CGMCC No. 36050. The preparation method of the sterile fermentation filtrate of the streptomyces fulvus C-21 comprises the following steps: inoculating a strain C-21 to an oat culture medium plate in a streak manner, culturing for 7 days at 28 DEG C, scraping spores on the surface of the culture medium, adding sterile water to prepare a spore suspension with the concentration of 108 / mL, inoculating the spore suspension into a millet immersion culture solution according to the inoculum size of 2%, and culturing for 7 days at the temperature of 28 DEG C to obtain the sterile fermentation filtrate of the streptomyces fulvus C-21. Performing shaking culture for 7 days in a constant-temperature shaking table at the temperature of 28 DEG C and the speed of 180 r / min, centrifuging for 30 minutes at the speed of 12000 r / min, and filtering supernate by using a 0.22 mu m microporous filter membrane to obtain sterile fermentation filtrate. The streptomyces fulvus C-21 can generate extracellular enzymes such as cellulase, protease and amylase, sterile fermentation filtrate of the streptomyces fulvus C-21 can destroy mycelial morphology and cell membrane integrity of the tomato late blight bacteria, mycelial growth, sporangium germination and zoospore release of the tomato late blight bacteria are remarkably inhibited, and the streptomyces fulvus C-21 has a good control effect on the tomato late blight.

The application provides a kelp breeding performance evaluation method based on sporangium spore dissemination parameters and application, and belongs to the technical field of seaweed cultivation. The method comprises the following steps: S1, taking mature kelp samples, washing, and air-drying treatment to obtain pretreated kelp; S2, taking kelp tissue blocks from the pretreated kelp obtained in step S1, loading the kelp tissue blocks into a mesh bag, immersing the kelp tissue blocks in seawater, promoting the kelp tissue blocks to disseminate, and collecting zoospore liquid; S3, measuring sporangium spore dissemination parameters in step S2; S4, calculating dissemination capacity according to the sporangium spore dissemination parameters obtained in step S3, and evaluating the breeding performance of the kelp according to the dissemination capacity. The above method can quantify the sporangium spore dissemination characteristics of kelp, systematically evaluate the breeding potential of kelp, and improve the breeding efficiency and yield of kelp.

The application concerns a microbiological system supporting the consolidation of sand and sandy soils through biocementation, utilizing a mixture of bacteria from the Sporosarcina genus, including Sporosarcina sp. ANT_H38, deposited in the Polish Collection of Microorganisms (PCM) at the Institute of Immunology and Experimental Therapy of the Polish Academy of Sciences in Wrocław under number B / 00547, and Sporosarcina pasteurii DSM 33. The goal is to stabilize and strengthen soil structures through natural mineralization, which increases the substrate's load-bearing capacity. The process involves culturing the bacteria on LB medium, diluting them and combining them with urea, and then applying them to sand or soil with the addition of a cementing medium. The system also operates effectively at low temperatures, enabling broad applications in construction and environmental protection. The application also concerns a method for producing the microbiological system and a method for supporting the consolidation process of sand and / or sandy soils using this system.

The application provides a kind of double (2,3-dihydroxy benzamide) compound and its preparation method and application, belong to medical technology field.The application obtains a series of double (2,3-dihydroxy benzamide) compound with novel structure and good inhibiting effect on cholestatic liver disease and liver fibrosis by structural modification of sporangium chelae, the compound can significantly inhibit the expression of hepatic stellate cell COL1A1 mRNA in vitro, has strong anti-liver fibrosis effect;In vivo, it can significantly alleviate the liver injury related biochemical indicators of 3,5-diethoxycarbonyl-1,4-dihydro-2,4,6-trimethylpyridine (DDC) induced cholestatic liver disease mouse model, inhibit the expression of liver fibrosis related genes, reduce the content of liver collagen fiber, inhibit liver inflammation and bile duct response, and can be used for treating cholestatic liver disease and liver fibrosis.

The invention provides a monoclonal antibody for specifically recognizing cysts and oocysts / sporangiums of sheep sarcocysts and application of the monoclonal antibody, and the monoclonal antibody provided by the invention can generate positive reaction aiming at soluble antigens of adult holoproteins of sarcocysts tenella and soluble antigens of oocysts / sporangiums of the sarcocysts tenella. The two key development stages of adults and oocysts / sporangiums are covered at the same time. A complete parasite life cycle detection system can be established, the clinical sample detection rate is remarkably improved (experiment shows that the clinical sample detection rate is improved by 35-40%), and the detection window period is 7-10 days earlier than that of a traditional method. Meanwhile, the ELISA detection kit and the detection method thereof provided by the invention are high in sensitivity and convenient to operate, can greatly improve the detection efficiency, and have a good industrial application prospect.

This invention discloses an oligosaccharide syltransferase STT3 subunit from *Phytophthora sojae*, its encoding gene, and its applications. The protein sequence of the oligosaccharide syltransferase STT3 subunit provided by this invention is shown in Sequence 2; its encoding gene is shown in Sequence 1. Experiments have demonstrated that the protein provided by this invention plays an important role in the growth and development of *Phytophthora sojae*, specifically manifested in the slowed mycelial growth, reduced number of sporangia and zoospores, and decreased pathogenicity of *Phytophthora sojae* after its deletion. These conclusions provide a technical basis for exploring the molecular mechanisms of *Phytophthora sojae* development and pathogenicity, and provide molecular targets for the future development of novel fungicides.

The invention relates to a kelp development stage-CIELAB parameter standard database and a method for regulating and monitoring the maturity of kelp sporangium by using the same, and belongs to the technical field of large seaweed cultivation and seedling raising. The method comprises the following steps: firstly, establishing a development stage-CIELAB parameter standard database, and then measuring the CIELAB value of a sample to determine the maturity of kelp sporangium; the development stage of a sample is judged according to the established standard database, and the development process of kelp is regulated and controlled by using light quality according to production requirements, so that the requirements of kelp seedlings at different time can be met, the season dependence bottleneck of traditional seedling culture is solved, and cross-season and multi-batch efficient production of winter seedlings and the like becomes possible.

The utility model discloses a downy mildew spore collecting device which comprises a holding assembly, a collecting assembly, a filtering assembly, a catching assembly and a suction assembly, and the holding assembly is sequentially provided with a filtering bin, a catching bin, an air pump bin and a holding part from front to back; the filtering assembly is installed in the filtering bin, the capturing assembly is installed in the capturing bin, the suction assembly is arranged in the air pump bin, the collecting assembly is installed in front of the filtering assembly, and the suction assembly is electrically connected with the power source. According to the device, the holding assembly, the collecting assembly, the filtering assembly, the capturing assembly and the suction assembly are integrated, so that efficient collection of downy mildew spores is achieved. Due to the innovative structural design, the collecting process is quicker and more convenient, and the collecting efficiency is remarkably improved. According to the device, the combination of the filtering assembly and the capturing assembly is adopted, the collection concentration of sporangium is effectively improved, an additional centrifugal concentration step is not needed, the operation process is simplified, and time and cost are saved.

The invention discloses a glucosidase protein (GLS1) from phytophthora plant pathogenic oomycetes as well as a coding gene and application thereof. The PsGLS1 protein provided by the invention is a protein of which the sequence is as shown in SEQ ID No. 1 in Phytophthora sojae, and the PsGLS1 protein is a protein of which the sequence is as shown in SEQ ID No. 2 in Phytophthora sojae; the coding gene of the gene is shown as SEQ ID No.2. Experiments prove that the protein provided by the invention plays an important role in the growth and development process of phytophthora sojae, and after the PsGLS1 gene is knocked out, the mycelial growth rate, the sporangium number, the zoospore yield and the resting spore germination rate of the phytophthora sojae are obviously reduced, and the pathogenicity of pathogenic bacteria is obviously influenced. Glucosidase inhibitor mispermine has an inhibition effect on the growth of phytophthora sojae hyphae. In conclusion, the PsGLS1 gene has very high application value in control of epidemic disease occurrence and epidemic caused by phytophthora, and a molecular target is provided for research and development of novel bactericides in the future.

The invention provides a method for detecting the activity of coccidian oocysts and judging the quality grade of the coccidian oocysts, and the method for detecting the activity of the coccidian oocysts comprises the following steps: obtaining the sporozoite escape rate M of a to-be-detected coccidian oocyst sample, and determining whether the activity of the coccidian oocysts is qualified or not according to the sporozoite escape rate M, the method for obtaining the sporozoite escape rate M comprises the following steps: (1) preparing a coccidian oocyst suspension of a coccidian oocyst sample; (2) carrying out wall breaking on oocysts in the coccidium oocyst suspension to enable the oocysts to release sporangiums so as to obtain a sporangium suspension; (3) digesting the obtained sporangium suspension by using digestive juice, so that sporangium releases sporozoites to obtain a sporozoite suspension, counting the sporozoites and sporangium in the sporozoite suspension, and marking the number of the sporozoites and sporangium as B and C respectively, and (4) calculating the sporozoite escape rate M: when the sporozoite escape rate M is greater than or equal to 70%, determining that the activity of the coccidium oocysts is qualified.

This invention discloses a glucosidase protein (GLS1) from *Phytophthora*, a plant pathogenic oomycete, its encoding gene, and its applications. The PsGLS1 protein provided by this invention is effective against *Phytophthora soybean* (…). Phytophthora sojae The protein in this invention is shown in SEQ ID No. 1; its encoding gene is shown in SEQ ID No. 2. Experiments have demonstrated that the protein provided by this invention plays an important role in the growth and development of *Phytophthora soybeani*. PsGLS1 Gene knockout significantly reduced the mycelial growth rate, sporangium number, zoospore yield, and resting spore germination rate of *Phytophthora soybeani*, and also significantly affected the pathogenicity of the fungus. The glucosidase inhibitor sericite also inhibited the mycelial growth of *Phytophthora soybeani*. In summary, this invention… PsGLS1 Genes have high application value in controlling the occurrence and spread of diseases caused by Phytophthora, and provide molecular targets for the development of new fungicides in the future.

The invention discloses a tissue culture method for botrychium ternatum spore induced prothallium. The culture method comprises the following steps: collecting botrychium ternatum with sporangium, drying, sieving and collecting spores, sequentially carrying out multiple times of disinfection and cleaning pretreatment on the collected spores by using 75% ethanol, sterile water, 2% sodium hypochlorite and the like in a sterile environment, uniformly coating a culture medium with treated spore suspension, and carrying out observation culture, the time from spore inoculation to prothallium formation is 24-26 days, and the time from spore inoculation to juvenile sporophyte formation is 47-63 days. The culture medium consists of the following components: a 1 / 8 MS culture medium, 5g / L of sucrose and 8g / L of agar. The culture medium is simple to prepare, can effectively induce spore germination and prothallus proliferation of the botrychium ternatum, can be further cultured to obtain sporophytes, can also be used for acclimatization and transplantation, shortens the seedling raising period, further realizes large-scale cultivation of the botrychium ternatum, and has important significance for expanded application of pteridophyte.

The invention provides a method for preserving germplasm resources of adiantum obtusifolium, which comprises the following steps: step 1, collecting uncracked sporangiums on sporophytes of adiantum obtusifolium, treating the sporangiums with 0.05-0.1% mercuric chloride, then cleaning the sporangiums, and inoculating the sporangiums to a culture medium; 2, transferring the sporangium on the culture medium to a germination culture medium after the sporangium passes through a pollution period, culturing until the sporangium germinates to form prothalli, then transferring the prothalli to a proliferation culture medium, and culturing and proliferating to form proliferated prothalli; 3, prothalli needing to be preserved are selected from the proliferated prothalli and transferred to a preservation culture medium to be preserved and cultured, and preserved adiantum obtusifolium is obtained; the preservation culture medium is a 1 / 8 MS culture medium containing 0.03 to 0.05 mg / L of 6-BA, 0.1 to 0.4 mg / L of NAA, 1.5 to 3.0 mg / L of IBA and 0.2 to 0.4 mg / L of CCC.

The application provides application of chitin in early infection of plasmodiophora brassicae in prevention and control of brassica root tumour disease, and is based on the technology and application of blocking chitin synthesis of zoosporangium in early infection of plasmodiophora brassicae to prevent and control brassica root tumour disease. It is proved by experiments that the zoosporangium in early infection of plasmodiophora brassicae contains chitin, and the chitin is synthesized by plasmodiophora brassicae chitin synthase genes PbCHS3 and PbCHS7, thereby providing a new target for prevention and control of brassica root tumour disease. Through expression of PbCHS3 and PbCHS7 silencing vectors, exogenous small RNA treatment or application of polyoxin, which can block chitin synthesis, in arabidopsis and rape, brassica root tumour disease can be prevented and controlled efficiently, and key technology and method for prevention and control of brassica root tumour disease are provided.

The present application relates to the field of microbial culture, in particular to a culture medium preparation and culture method for inducing the germination of blood ear spores into mycelium. The culture medium ratio comprises 500-700 mL of 200 g potato extract, 300-500 mL of blood mark rigid leather fungus fermentation supernatant, 20 g of glucose, 20 g of agar, 0.46 g of potassium dihydrogen phosphate, 1.0 g of calcium superphosphate, 0.5 g of magnesium sulfate, 2 g of fish peptone, 20 g of soybean hull, and 1 mg of VB6. The blood ear spores are inoculated into the culture medium, and cultured in a constant temperature incubator at 20-23.5 DEG C. Mycelium can be observed around the blood ear spores after 15-20 days. Therefore, the stable germination of blood ear spores into mycelium is successfully induced, and the problem of obtaining pure mycelium of blood ear is solved. From the aspect of cultivation, the difficulty of strain preparation in artificial cultivation of blood ear is reduced. From the aspect of research, the implementation of the present application has great significance for the related basic biological research of blood ear.

The application discloses a culture method for propagating Brachythecium populeum, and belongs to the technical field of Brachythecium populeum tissue culture. The application uses sporangium as an explant to propagate Brachythecium populeum, and quickly obtains Brachythecium populeum spores with a high germination rate, establishes a regeneration system of Brachythecium populeum, lays a foundation for large-scale propagation of Brachythecium populeum, shortens the propagation cycle of Brachythecium populeum, and better maintains excellent properties. The application first establishes a method for culturing Brachythecium populeum by using spores, fills the blank of the prior art, and provides a technical basis for propagation, seed preservation, preparation of protoplasts, recombination experiments and gametophyte culture of Brachythecium populeum.

This invention discloses an arginine methyltransferase protein from *Phytophthora* plant pathogenic oomycetes, its encoding gene, and its applications. The arginine methyltransferase protein provided by this invention is shown in sequences 6-8; its encoding gene is shown in sequences 1-3. Experiments have demonstrated that the protein of this invention plays an important role in the growth, development, and pathogenicity of *Phytophthora capsici*, a typical plant pathogenic oomycete. The absence or reduced expression level of this protein leads to slower mycelial growth rate, reduced sporangium production, decreased zoospore numbers, and reduced pathogenicity of *Phytophthora capsici*. Corresponding arginine methyltransferase inhibitors exhibit varying degrees of activity against typical plant pathogenic oomycetes such as *Phytophthora capsici*, *Phytophthora soybeani*, and *Phytophthora pathogenica*. The gene provided by this invention has significant application potential in the prevention and control of crop blight caused by *Phytophthora* plant pathogenic oomycetes. Novel fungicides developed based on this protein as a target have important practical significance for controlling the occurrence and spread of crop blight caused by *Phytophthora*.

This invention discloses a comprehensive identification method for *Peronobacter spp.* strain QH, belonging to the field of plant pathogen identification technology. The method includes the following steps: obtaining a sample of the *Peronobacter spp.* strain to be identified; extracting morphological characteristics of hyphae, sporangia, and conidiophores; extracting key morphological parameters and generating a morphological feature vector; amplifying its housekeeping gene fragment using polymerase chain reaction and sequencing to obtain the nucleotide sequence; calculating sequence similarity and generating a molecular feature vector; performing comprehensive matching and judgment, and outputting the identification result; generating an identification report, and combining it with strain information in a database for source tracing analysis and risk warning. This invention solves the problems of traditional identification methods relying on single indicators, strong subjectivity, and difficulty in distinguishing intraspecific genetic variations by multimodal fusion of morphological quantitative characteristics and molecular biological marker characteristics. It achieves accurate, objective, and automated identification of *QH* strains, providing data support for disease source tracing and control decisions.

The invention provides a strain of bacillius pink and application thereof in promoting formation of sporangia branches of porphyra haitanensis. The preservation number of the provided bacillius pink is CCTCC (China Center for Type Culture Collection) NO: M 20242610. According to the method, the pink rod-shaped strain is added into a porphyra haitanensis protonema culture system, so that the development of the sporangium branches can be effectively promoted, the formation proportion of the sporangium branches can be remarkably increased to 48.8% after culture is performed for 4 weeks, directional ripening under manual intervention is realized, the defects of long period and poor stability of a traditional environment regulation and control means are overcome, and the method is suitable for large-scale popularization and application. The conchospore diffusion consistency and quality can be improved, and a new technical approach is provided for porphyra haitanensis seedling production.

The application discloses a method for reversibly inhibiting the maturation of a Porphyra haitanensis filament, and belongs to the technical field of Porphyra haitanensis breeding. The method comprises adding DMTU to a filament culture medium, and provides a method for inhibiting the maturation of a Porphyra haitanensis filament. When maturation is started, the filament culture solution containing DMTU is used to promote the maturation of the filament, so that the growth of the Porphyra haitanensis filament and the formation of sporangial branches can be effectively inhibited. The inhibiting effect is reversible, and normal maturation can be restored after the treatment is stopped. The inhibiting effect is immediately released, and then the precise regulation of the maturation process of the filament is realized, and the large number of "miscarriages" of chlamydospores is effectively prevented. Therefore, the maturation inhibition method has a good application prospect, and has important practical significance and application value for improving the technical level of the Porphyra haitanensis breeding industry and guaranteeing the sustainable development of the industry.

The invention relates to the field of microbial culture, in particular to a preparation method of a culture medium for inducing tremella sanguinea spores to germinate hyphae and a culture method. The culture medium is prepared from 500-700 mL of an extracting solution prepared from 200 g of potatoes, 300-500 mL of stereum bloodstain fermentation supernate, 20 g of glucose, 20 g of agar, 0.46 g of monopotassium phosphate, 1.0 g of calcium superphosphate, 0.5 g of magnesium sulfate, 2 g of fish peptone, 20 g of soybean hulls and 1 mg of VB61. According to the method, tremella sanguinea spores are inoculated into the culture medium and cultured in a constant-temperature incubator at the temperature of 20-23.5 DEG C, and hyphae can germinate around the tremella sanguinea spores after 15-20 days, so that the tremella sanguinea spores are successfully induced to stably germinate into the hyphae, and the problem that pure tremella sanguinea hyphae are difficult to obtain in the aspect of tremella sanguinea research at present is solved. And in the aspect of cultivation, the difficulty of strain preparation in the artificial cultivation technology of tremella sanguinea is reduced. From the aspect of research, the implementation of the invention has great significance on the basic biological research work related to tremella sanguinea.