Breeding method of cibotium barometz spores

A technology of golden retriever and ridge spores, which is applied in the breeding field of golden retriever ridge spores, can solve the problems of high cost of rhizome section propagation and breeding seedlings, complicated breeding of dog ridge seedlings, and increased demand for dog ridge, and achieves Protect fern resources, avoid digging, and have the same effect on physiological age

Inactive Publication Date: 2018-02-02
傅明尧
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the variety of Chinese patent medicines containing dog spines in domestic pharmaceutical companies, the demand for dog spines has increased, leading to crazy digging among the people, and wild resources have become increasingly scarce. In addition, the breeding of artificially planted dog spines is complicated. The cost of breeding seedlings by cutting stems is high and the cycle is long, so it is not suitable for promotion

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A kind of breeding method of golden retriever dog ridge spore, comprises the steps:

[0020] (1) Collection of spores: pick the pinnae with sporangia group on the wild golden retriever plant, put it in a clean bag and dry it in the shade naturally, remove impurities after the sporangia fall off naturally, collect the sporangia, and use 0.1% mercuric chloride solution to act After 4 minutes, rinse with sterile water 3 times, dry and collect in a sterile bag;

[0021] (2) pre-cultivation treatment: the aseptic bag containing sporangia obtained in step (1) was placed in 2°C for preservation for 340 days, and the sporangia preserved at low temperature was sterilized for 40 seconds with 75% alcohol; the spore germination medium Carry out high-pressure moist heat sterilization with conventional culture medium;

[0022] (3) Germination and cultivation: inoculate the sporangia sterilized in step (2) into a tissue culture tank equipped with a spore germination medium, and carry...

Embodiment 2

[0025] A kind of breeding method of golden retriever dog ridge spore, comprises the steps:

[0026] (1) Collection of spores: pick the pinnae with sporangia group on the wild golden retriever plant, put it in a clean bag and dry it in the shade naturally, remove impurities after the sporangia fall off naturally, collect the sporangia, and use 0.1% mercuric chloride solution to act After 6 minutes, rinse with sterile water 5 times, dry and collect in a sterile bag;

[0027] (2) Pre-cultivation treatment: the aseptic bag containing sporangia obtained in step (1) was placed in 6°C for preservation for 380 days, and 70% alcohol was used to disinfect the sporangia preserved at low temperature for 40 seconds; to the spore germination medium Carry out high-pressure moist heat sterilization with conventional culture medium;

[0028] (3) Germination and cultivation: inoculate the sporangia sterilized in step (2) into a tissue culture tank equipped with a spore germination medium, and ...

Embodiment 3

[0031] A kind of breeding method of golden retriever dog ridge spore, comprises the steps:

[0032] (1) Collection of spores: pick the pinnae with sporangia group on the wild golden retriever plant, put it in a clean bag and dry it in the shade naturally, remove impurities after the sporangia fall off naturally, collect the sporangia, and use 0.1% mercuric chloride solution to act After 5 minutes, rinse with sterile water 5 times, dry and collect in a sterile bag;

[0033] (2) pre-cultivation treatment: the aseptic bag containing the sporangia obtained in step (1) is placed in 4 DEG C and stored for 360 days, and the sporangia preserved at low temperature is sterilized with 75% alcohol for 30 seconds; the spore germination medium Carry out high-pressure moist heat sterilization with conventional culture medium;

[0034] (3) Germination and cultivation: inoculate the sporangia sterilized in step (2) into a tissue culture tank equipped with a spore germination medium, and carry...

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PUM

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Abstract

The invention discloses a method for breeding golden-haired dog ridge spores. Pick pinnae with sporangia clusters on wild golden-haired dog plants, collect the sporangia, disinfect and wash them, then dry them, collect them in a sterile bag, and then put the aseptic After 340-380 days of low-temperature storage in the bag, sterilize the sporangia with 70%-75% alcohol, and then inoculate them into the spore germination medium for germination culture; when the prothallus grows, inoculate the prothallus into the conventional culture medium Carry out gametophyte transformation and culture; when the gametophyte grows the third leaf, transfer the culture tank to the room, open the lid of the culture tank for seedling cultivation, then add nutrient soil for field transplant adaptation, and finally carry out field transplant. The method for cultivating the golden-haired dog's spores of the present invention has a simple operation process, the two-step method cultivates the golden-haired dog's ridge seedlings, saves costs, the seedlings grow neatly, and are suitable for large-scale cultivation.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a method for breeding golden retriever spores. Background technique [0002] Cibotium barometz (L.) J.Sm. is a fern of the clamshell fern family. It is recorded in the "Dictionary of Traditional Chinese Medicine" that Cibotium barometz is born on the side of the mountain foot, or in the acidic soil in the shade of the forest. Distributed in the southwest, south, southeast of my country, Henan, Hubei and other places, mainly produced in Sichuan, Fujian, and Zhejiang. In addition, it is also produced in Guangxi, Guangdong, Guizhou, Jiangxi, Hubei and other places. [0003] The rhizome of Golden Retriever Dog’s Ridge, which is dried and depilated, is the traditional Chinese medicine Dog’s Ridge, which was first recorded in "Shen Nong’s Herbal Classic", and has been recorded in all previous editions of "Chinese Pharmacopoeia". , for the treatment of soreness in the waist ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 傅明尧
Owner 傅明尧
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