12 results about "Moist heat sterilization" patented technology

Disclosed in the present invention are a composition comprising microspheres and crosslinked hyaluronic acid or a salt thereof, and a preparation method therefor and a use thereof. The composition comprises crosslinked hyaluronic acid or a salt thereof, microspheres, a phosphate buffer, and non-crosslinked hyaluronic acid or a salt thereof. In the present invention, under a condition of a large amount of an alkali solution, a mixture of high- and low-molecular-weight hyaluronic acid or a salt thereof is used to form a loose structure; under a condition of a small amount of an alkali solution, low-molecular-weight hyaluronic acid or a salt thereof is used to form a compact network structure; the loose structure and the compact network structure are mixed to continue a crosslinking reaction to form a gel having a soft elastic mosaic structure in which loose pores wrap elastic hard spheres; then the gel is mixed with the microspheres, the entry of rigid microspheres in the gel enables the composition to have both a soft elastic function and rigid support performance, and the soft elastic function and the rigid support performance have a synergistic effect; the gel can also effectively protect the microspheres during moist heat sterilization, reducing the degradation of the microspheres, prolonging the effect of the microspheres in stimulating collagen regeneration, making applicable skin layers and sites to be filled more extensive, and improving the safety of the composition in use.

This invention provides a method for preparing rocuronium bromide injection. By optimizing the conventional moist heat sterilization process, specifically by increasing the initial temperature of the heating process and decreasing the initial temperature of the cooling process, the overall exposure time of the injection to high temperatures is significantly shortened while maintaining the sterilization time at 121°C, thus significantly improving the quality stability of rocuronium bromide injection. Multiple batches of samples from this invention have met the related substance limits for rocuronium bromide injection as specified in the Chinese Pharmacopoeia, after accelerated stability and long-term stability studies.

This application provides a sodium hyaluronate gel and its preparation method, belonging to the field of biomedical materials, aiming to solve the problem of severe performance degradation of cross-linked sodium hyaluronate gels during moist heat sterilization. The gel contains cross-linked sodium hyaluronate and is compounded with a solvation shell-building agent and a high-temperature free radical scavenger. These two agents synergistically constitute a dual protection system, jointly protecting the gel structure. The preparation method includes matrix cross-linking, in-situ loading of the protection system through dialysis equilibrium, homogenization, and terminal moist heat sterilization. After moist heat sterilization at 121°C, the gel of this invention exhibits a high storage modulus retention rate, demonstrating excellent thermal stability and synergistic effects, ensuring stable product performance.

The present application relates to the technical field of microorganism or culture thereof processing, and particularly relates to a method for breeding pleurotus ostreatus mycelium by using highland barley as a substrate. The method is as follows: highland barley is crushed and sieved, then water is added to prepare highland barley slurry, and a complex enzyme system composed of amylase and cellulase is added to perform liquid enzymolysis to obtain an enzymolysis substrate; lactic acid bacteria strains are inoculated into the enzymolysis substrate to perform solid-state fermentation to obtain a fermentation substrate; the fermentation substrate is subjected to moist heat sterilization treatment to obtain a sterilized substrate; the sterilized substrate is inoculated with pleurotus ostreatus original seeds, and is placed in a culture environment for culture to obtain pleurotus ostreatus mycelium. The scheme breaks the physical barrier of the fiber network of the highland barley epidermis and the starch crystal structure of the endosperm by using the complex enzyme system and lactic acid bacteria fermentation, and the substrate is pre-degraded before the mycelium is inoculated, so that the mycelium can directly absorb free carbon sources, the germination time is shortened, the daily growth rate is improved, the residual sugar rate of the substrate is reduced, and the dry weight biomass of the mycelium is increased.

According to an embodiment of the present disclosure, the room-temperature stable, unbuffered rocuronium injection formulation for intravenous administration comprises of 1% w / v of rocuronium bromide, a pH adjusting agent comprising hydrochloric acid at a concentration between 12.5 mMol and 20 mMol for the adjustment of pH between 3.5 to 4.5 and a quantity sufficient of water to make the formulation to 1 mL. The proposed formulation of the present disclosure is stabilized through optimization of pH of the formulation using only hydrochloric acid and / or sodium hydroxide as the pH adjusting agents without use of any buffering agents is suitable for moist heat sterilization. Further, the rocuronium injection formulation of the present disclosure contains lower than 3% of rocuronium related compound C, more preferably less than 2% over the stability period at room temperature conditions.

The application provides a polyvinylidene fluoride separation membrane and a preparation method and application thereof. The preparation method of the polyvinylidene fluoride separation membrane comprises the following steps: mixing polyvinylidene fluoride, a solvent and an additive, stirring and dissolving uniformly, standing and defoaming, and preparing a membrane preparation liquid; then starting a flat plate film casting machine, controlling the temperature and air humidity of a film casting area, setting the moving speed of a scraper and the film casting thickness, pouring the casting liquid uniformly on the flat plate with a substrate on the surface for film casting; then immersing in a coagulation bath for exchange; after the exchange, moving into pure water for standing and solidification to obtain a wet original membrane; performing heat treatment and wet heat sterilization treatment on the wet original membrane; and finally drying the wet original membrane after the wet heat sterilization. The compact pore structure of the membrane is opened through heat treatment, the porosity is effectively improved, the effect of cleaning the membrane structure is achieved, the membrane is sterilized and heat set through wet heat sterilization treatment, and the structure change of a later assembly after leaving the factory for wet heat sterilization is avoided, so that the separation precision is changed.

This invention belongs to the field of biomedical material preparation technology, specifically disclosing a method for moist heat sterilization of recombinant collagen fillers. The method includes: preparing a protective solution from modified trehalose and proline, then mixing it with homogenized recombinant collagen hydrogel, followed by staged temperature-controlled moist heat sterilization. This invention modifies trehalose by succinylation, introducing carboxyl groups onto the trehalose molecule, enabling electrostatic interactions with cationic groups on the surface of collagen molecules. During moist heat sterilization, free proline guides the heat-denatured collagen to refold into its natural triple helix structure. The preheating stage allows the collagen to gradually adapt to the thermal environment, avoiding conformational shock damage caused by sudden temperature increases, effectively shortening the high-temperature sterilization time, and significantly reducing the cumulative heat effect on molecular structure. This method is simple to operate, requires no special equipment, and has good prospects for industrial application.

The utility model provides a kind of wet heat sterilization respirator bag with tweezers loading support, including the U-shaped support frame formed by two vertical rods and a horizontal rod, fixedly connected with suspension rod and fixed sheet between two vertical rods, a plurality of insertion holes for tweezers foot insertion are set on fixed sheet, each insertion hole is sequentially arranged according to same interval. The utility model is used to solve the problem that tweezers cannot be fixed in the placing mode in respirator bag, and the heat treatment sterilization effect has difference.

The application relates to the technical field of organic acid production, and particularly discloses a butyric acid non-full sterilization production process. The butyric acid non-full sterilization production process comprises the following steps: sub-culturing: wet heat sterilization is carried out on a seed tank and a seed culture medium, and after sterilization, butyric acid production bacteria are inoculated to culture, so that pure high-activity seed liquid is obtained; fermentation culture medium configuration: a glucose-based fermentation culture medium is configured, and the glucose-based fermentation culture medium is transferred into a fermentation tank; non-full sterilization fermentation: the pure high-activity seed liquid is inoculated into the fermentation tank, a micro-positive pressure environment in the tank is maintained during fermentation, and butyric acid fermentation is carried out; post-treatment: after fermentation is completed, the fermentation product is extracted and refined, and butyric acid product is obtained.

The utility model discloses a kind of sterilization equipment of sea mushroom planting substrate, including sterilization outer cavity, further including steam generator and sterilization mechanism, the sterilization mechanism is arranged in sterilization outer cavity;The sterilization mechanism includes sterilization inner cavity, and the connecting pipe is welded in sterilization inner cavity side end, and microwave generator is arranged in the connecting pipe;A kind of sterilization equipment of sea mushroom planting substrate is provided with breathable filter screen, sterilization inner cavity, lid, second circulation pipe, flow guide groove and steam generator, high-temperature water vapor is generated by steam generator, microorganism cell structure is destroyed using moist heat sterilization principle, then cooperate microwave generator in connecting pipe, threaded connection bottom pipe and threaded connection bottom pipe, utilize the high-frequency electromagnetic field of microwave, improve sterilization efficiency, reduce miscellaneous bacteria pollution risk, realize internal uniform heating, further kill residual microorganism, double sterilization process can shorten sterilization time, improve sterilization efficiency, reduce miscellaneous bacteria pollution risk.

The invention provides a preparation method of a pelvic inflammation cleaning oral liquid, which comprises the following steps: diluting clear paste obtained after water extraction and alcohol precipitation of pelvic inflammation cleaning medicinal materials, atomizing the diluted clear paste into 10-50 [mu] m liquid drops by a high-pressure nozzle, instantly freezing the liquid drops in liquid nitrogen at the temperature of-40 DEG C, and carrying out sublimation drying for 24-48 hours under the conditions that the vacuum degree is 10-50Pa and the temperature is-40 DEG C to-20 DEG C to obtain a raw material medicine. And finally, adding the flavoring agent and the preservative to prepare the composition. Compared with a traditional process, the method can improve the stability and the quality controllability of the oral liquid finished product, meanwhile, the degradation of effective components of the raw material medicines in the whole process of drying and moist heat sterilization can be inhibited from the source, and the retention rate of the effective components is remarkably improved to 95% or above.

The application discloses a heat-resistant and stable recombinant humanized type VII collagen as well as a preparation method and application thereof, and belongs to the technical field of genetic engineering. The amino acid sequence of the recombinant humanized type VII collagen is formed by connecting four type VII collagen functional fragments in series, and the coding nucleotide is obtained by Pichia pastoris preference optimization design. The sequence is inserted into a pPIC9K plasmid to construct a recombinant vector, and the engineering bacteria are obtained by electric transformation of Pichia pastoris GS115. The target protein rhCOL7 is prepared through fermentation and purification. The rhCOL7 protein has excellent thermal stability, can resist 115 DEG C moist heat sterilization, has no obvious degradation after being placed at 60 DEG C for 60 days, can significantly promote cell proliferation, migration and adhesion, and can specifically interact with type IV collagen. The application realizes efficient preparation of the recombinant humanized type VII collagen, the activity of which is high and the stability of which is good, and the recombinant humanized type VII collagen can be widely applied to the preparation of wound repair drugs, biological repair materials and cosmetics.