129 results about "Moist heat sterilization" patented technology

The invention discloses a preparation method of a high-adsorbability aluminum hydroxide adjuvant for livestock, which comprises the following steps: when an aluminum chloride solution reacts with alkali liquor, generating a colloidal suspension by combining pH two-step adjustment with dispersant addition; and after instant high-speed shearing and room-temperature aging, performing ultrafiltration purification to remove salts, and finally performing moist heat sterilization to obtain the suspension morphological adjuvant. The adjuvant is high in antigen adsorption rate, low in 72-hour desorption rate, large in specific surface area, uniform in particle and suitable for vaccines of pigs, cattle and poultry, and the problems that a traditional adjuvant is low in adsorption rate and poor in stability are solved.

The invention belongs to the technical field of medical and mechanical combinations, and discloses a high-permeability and low-sensitivity ripdicaine wound patch and a preparation method thereof.The high-permeability and low-sensitivity ripdicaine wound patch comprises a wound patch body, and the wound patch body is prepared from, by mass, 0.1%-0.4% of sodium hyaluronate, 1.5%-3.5% of lidocaine, 1.5%-3.5% of prilocaine, 3%-10% of solvent, 0.5%-8% of emulsifier, 2%-10% of humectant, 0.1%-5% of soothing agent, 0.1%-1.5% of stabilizer, 0.1%-1% of thickener and the balance water. According to the present invention, the total amount of the raw materials is 100%, the total amount of the raw materials is 100%, the total amount of the raw materials is 100%, the total amount of the raw materials is 100%, the total amount of the raw materials is 100%, the total amount of the raw materials is 100%, the total amount of the raw materials is 100%, the total amount of the raw materials is 100%, and the total amount of the raw materials is 100%. And the anesthetic has the advantages of quick effect taking, low sensitivity, capability of resisting moist heat sterilization and good stability.

The invention relates to a cycloolefin copolymer composite material as well as a preparation method and application thereof. The cycloolefin copolymer composite material is prepared from the following raw materials: a cycloolefin copolymer, a thermoplastic elastomer, a nucleating agent and a flow promoter, the total mass of the cycloolefin copolymer and the thermoplastic elastomer is 100%, and the content of the thermoplastic elastomer is 0.5-4%. The cycloolefin copolymer composite material disclosed by the invention has better impact resistance and drop resistance, eliminates microcracks after high-temperature moist heat sterilization, is high in material transparency and excellent in light transmittance, and can be well applied to medicine packaging and medical equipment.

The invention provides a preparation method and application of a suspension containing a PDE-4 or PDE-3 / 4 inhibitor for sterile inhalation. The preparation method comprises the following steps: dispersing a PDE-4 or PDE-3 / 4 inhibitor in injection water containing an excipient, and carrying out high-pressure moist heat sterilization to obtain a PDE-4 or PDE-3 / 4 inhibitor dispersion liquid; dissolving the long-acting beta2-receptor stimulant and / or the long-acting muscarinic receptor antagonist, sterilizing and filtering to obtain a mixed solution; and mixing the PDE-4 or PDE-3 / 4 inhibitor dispersion liquid with the mixed solution, dispersing, and carrying out sterile filling. The medicinal preparation prepared by the invention is a sterile inhalation suspension, and the sterile inhalation suspension containing the PDE-4 or PDE-3 / 4 inhibitor is high in bioavailability, good in patient medication compliance and good in preparation stability.

The invention discloses a composition containing microspheres and cross-linked hyaluronic acid or salt thereof as well as a preparation method and application of the composition. The composition comprises cross-linked hyaluronic acid or salt thereof, microspheres, a phosphate buffer solution and non-cross-linked hyaluronic acid or salt thereof. Mixed hyaluronic acid or salt thereof with high and low molecular weight is used under the condition of large amount of alkali solution to form a loose structure, hyaluronic acid or salt thereof with low molecular weight is used under the condition of small amount of alkali solution to form a compact network structure, and the two are mixed to continue cross-linking reaction to form a cross-linked structure. The preparation method comprises the following steps: firstly, forming gel with a soft elastic embedded structure with loose pores wrapping elastic hard spheres, then mixing the gel with the microspheres, and enabling the composition to have a soft elastic function and a rigid supporting performance due to entry of the rigid microspheres in the gel, so that the composition and the rigid microspheres have a synergistic effect; in addition, the gel can effectively protect the microspheres during moist heat sterilization, reduce degradation of the microspheres and prolong the effect of stimulating collagen regeneration by the microspheres, the gel is suitable for more skin levels and filling parts, and the use safety of the composition is improved.

The invention belongs to the field of gene engineering. In particular to high-stability recombinant XVII type collagen as well as a preparation method and application thereof. The recombinant XVII type collagen is obtained by repeating an amino acid sequence (as shown in SEQ ID NO.1) from a natural human XVII type collagen peptide for 2-5 times. The recombinant XVII type collagen can also be secreted and expressed in a pichia pastoris expression system, the preparation method is simple, and the high-yield recombinant XVII type collagen can be obtained at low cost. The cell proliferation, migration and adhesion promoting activities of the recombinant XVII type collagen are obviously superior to those of commercially available XVII type collagen, a triple helix structure can be formed at room temperature, a 1% aqueous solution has high-temperature stability and moist heat sterilization stability, and the recombinant XVII type collagen can be widely applied to the fields of cosmetics and medical instruments.

The invention relates to the technical field of biological materials, in particular to a PDRN porous microsphere as well as a preparation method and application thereof. The PDRN porous microspheres are prepared from the following raw materials: PDRN, an oil phase matrix, a curing agent and metal salt, the curing agent is at least one of polyethylene glycol acrylate and polylysine; the metal salt is at least one of calcium chloride, zinc chloride, magnesium chloride and ferric chloride. The PDRN porous microsphere has the advantages of being controllable in size, excellent in disintegration resistance and excellent in swelling resistance, is high in moist heat sterilization tolerance and strength, can be used as a filling material for medical beauty or an ideal candidate material of a tissue engineering scaffold, and has potential application value.

The invention discloses cross-linked hyaluronic acid containing microspheres or salts thereof as well as a preparation method and application of the cross-linked hyaluronic acid. According to the invention, HA with high molecular weight and HA with low molecular weight are respectively crosslinked under the conditions of large amount of aqueous alkali and small amount of aqueous alkali, microsphere components are respectively introduced before crosslinking, and the hyaluronic acid with high molecular weight and the hyaluronic acid with low molecular weight or salts thereof form a loose structure containing microspheres under the condition of large amount of aqueous alkali. The low-molecular-weight hyaluronic acid or the salt thereof forms a compact rubber ball mixed network structure under the condition of a small amount of an alkali solution, and then the two are mixed and continuously crosslinked, so that the overall structure is more compact, the microspheres can be more uniformly dispersed in the formed multiple network structure, the elastic modulus of the system is improved, the microspheres can be more effectively wrapped, and the service life of the system is prolonged. After moist heat sterilization, the loss rate of elastic modulus is low, the change of pH caused by microsphere degradation is small, and the degradation time of the microspheres after being implanted into a body is prolonged, so that the effect of stimulating collagen regeneration by the microspheres is prolonged, and the overall stability is improved.

The invention discloses a poly-deoxyribonucleotide salt and a preparation method and application thereof, the content of the poly-deoxyribonucleotide salt is greater than 95%, the protein content is less than 1.0%, and after high-pressure moist heat sterilization, the dynamic viscosity reduction amount does not exceed 25%, and the elastic index G'reduction amount does not exceed 30%. The polydeoxyribonucleotide salt disclosed by the invention is simple in preparation process, short in production period, low in cost and suitable for large-scale industrial production; and the preparation process is mild, few impurities are introduced, the product structure is less damaged, and the preparation method is environment-friendly. The polydeoxyribonucleotide salt disclosed by the invention has good physical support performance, has the effects of promoting skin cell repair, resisting skin sensitivity, whitening, resisting inflammation, preventing alopecia and growing hair, and has a wide market prospect.

The invention discloses an oil-in-water vaccine adjuvant, an emulsion as well as a preparation method and application of the oil-in-water vaccine adjuvant, and relates to the technical field of vaccine adjuvant preparation. The adjuvant obtained by the invention is still not layered after being subjected to high-temperature and high-pressure moist heat sterilization. The contamination risk when the adjuvant is uniformly mixed after being subjected to high-temperature and high-pressure damp-heat degerming and layering is reduced, and vaccine manufacturers can conveniently carry out next-step production. The antigen and the adjuvant are mixed, and the prepared vaccine has higher immune efficacy than similar oil-in-water adjuvant vaccines. The oil-in-water adjuvant provided by the invention has good vaccine production and application prospects. When a vaccine composition is prepared on the basis of the oil-in-water vaccine adjuvant, high kinetic energy, high shearing, high-speed stirring emulsification and high-pressure homogeneous emulsification are not needed either, the emulsification process is greatly simplified, emulsification can be successful through low kinetic energy stirring, and vaccine production and use are more convenient and efficient.

The invention relates to the technical field of films, in particular to a preparation method and application of a waterproof and oil-proof ventilation valve for food packaging. The preparation method comprises the following steps: carrying out plasma activation treatment on the porous polytetrafluoroethylene-based membrane; preparing fluoroalkyl silane modified silicon dioxide nano particles; preparing fluorine-containing organic silicon-silicon dioxide sol containing vinyl; a cross-linking layer is constructed through vacuum impregnation; preparing a hydrophilic / hydrophobic comonomer solution; copolymerized chain grafting is initiated by ultraviolet light; and water-based fluorocarbon emulsion is sprayed on the surface for curing. The obtained breather valve has super-hydrophobic and oleophobic characteristics, keeps high air permeability and high water vapor permeability at the same time, has the air permeability retention rate exceeding 95% after 10 times of moist heat sterilization, and is suitable for balancing internal and external pressure and blocking water and oil phase liquid permeation in the high-temperature and high-pressure sterilization process of a food packaging container.

Disclosed in the present invention are a composition comprising microspheres and crosslinked hyaluronic acid or a salt thereof, and a preparation method therefor and a use thereof. The composition comprises crosslinked hyaluronic acid or a salt thereof, microspheres, a phosphate buffer, and non-crosslinked hyaluronic acid or a salt thereof. In the present invention, under a condition of a large amount of an alkali solution, a mixture of high- and low-molecular-weight hyaluronic acid or a salt thereof is used to form a loose structure; under a condition of a small amount of an alkali solution, low-molecular-weight hyaluronic acid or a salt thereof is used to form a compact network structure; the loose structure and the compact network structure are mixed to continue a crosslinking reaction to form a gel having a soft elastic mosaic structure in which loose pores wrap elastic hard spheres; then the gel is mixed with the microspheres, the entry of rigid microspheres in the gel enables the composition to have both a soft elastic function and rigid support performance, and the soft elastic function and the rigid support performance have a synergistic effect; the gel can also effectively protect the microspheres during moist heat sterilization, reducing the degradation of the microspheres, prolonging the effect of the microspheres in stimulating collagen regeneration, making applicable skin layers and sites to be filled more extensive, and improving the safety of the composition in use.

The invention belongs to the field of leukocyte-depleting filters, and particularly relates to a connecting method of a leukocyte-depleting filter and a connecting pipe suitable for moist heat sterilization. The invention aims to solve the technical problem that in the prior art, when a leukocyte-depleted filter is connected with a connecting pipe, due to the difference of thermal expansion coefficients of materials, interface stress concentration and damp-heat aging of an organic adhesive are caused, and then sealing failure, leakage or separation and the like occur after damp-heat sterilization. According to the connecting method for the leukocyte-depleted filter and the connecting pipe suitable for moist heat sterilization, the mechanical reliability of a connecting assembly is remarkably improved through the design of a composite interface of micro-bulge mechanical interlocking and hydrogel chemical bonding.

The invention provides an aluminum hydroxide adjuvant with a boehmite-like structure and a preparation method and application thereof.The preparation method comprises the steps that under the stirring condition, an aluminum salt solution and a sodium hydroxide solution are pumped into a 0.05%-0.5% sodium chloride solution heated to the set temperature at the same time, the pumping speed is adjusted, stirring is conducted for 1-2 h, and then the aluminum hydroxide adjuvant with the boehmite-like structure is obtained; controlling the ratio of hydroxyl to aluminum ions in the reaction system to be 2.5-3.5; when the concentration of aluminum ions in the reaction system reaches 0.07-0.09 M, stopping pumping the aluminum salt solution into the reaction system, continuously pumping the sodium hydroxide solution, and stopping pumping the sodium hydroxide solution when the pH value of the reaction system reaches 7.8-8.5; and adding 0.05-0.5% of a sodium chloride solution into the reaction system to a preparation amount, carrying out a hydrothermal reaction, discarding the supernatant, and carrying out moist heat sterilization to obtain the aluminum hydroxide adjuvant with the boehmite structure. The preparation method is reliable in process, good in batch-to-batch consistency and stable in quality.

The invention relates to the field of medical detection, in particular to a fluorescent sensing composition, a fluorescent sensing film and application thereof. According to the invention, by screening a plurality of fluorescent indicators and combinations thereof, an indicator formula with simple components and low cost is finally obtained. Based on the formula, by optimizing the preparation process, the high-efficiency fluorescent sensing film resistant to damp-heat sterilization is developed. After a microorganism culture bottle prepared on the basis of the membrane is inoculated with Gram-negative bacilli, positive cocci and yeast fungi representative quality control strains, experiments of full-automatic microorganism culture system fluorescence curve determination, Gram staining, microorganism mass spectrum identification and the like prove that the fluorescence sensing membrane is excellent in detection performance. The fluorescent sensing film provided by the research is applied to clinical microorganism detection, can provide technical support for blood flow infection diagnosis, and is of great significance in suppressing antibiotic abuse and improving prognosis of blood flow infection patients.

This invention provides a method for preparing rocuronium bromide injection. By optimizing the conventional moist heat sterilization process, specifically by increasing the initial temperature of the heating process and decreasing the initial temperature of the cooling process, the overall exposure time of the injection to high temperatures is significantly shortened while maintaining the sterilization time at 121°C, thus significantly improving the quality stability of rocuronium bromide injection. Multiple batches of samples from this invention have met the related substance limits for rocuronium bromide injection as specified in the Chinese Pharmacopoeia, after accelerated stability and long-term stability studies.

The application provides a preparation method and application of a sterile inhalation suspension containing a PDE-4 or PDE-3 / 4 inhibitor. The preparation method comprises dispersing the PDE-4 or PDE-3 / 4 inhibitor in injection water containing an excipient, high-pressure wet heat sterilization to obtain a PDE-4 or PDE-3 / 4 inhibitor dispersion; further comprising dissolving a long-acting beta2-receptor agonist and / or a long-acting muscarinic receptor antagonist, sterile filtration to obtain a mixed solution; mixing the PDE-4 or PDE-3 / 4 inhibitor dispersion with the mixed solution, dispersing and sterilely filling. The pharmaceutical preparation prepared by the application is a sterile inhalation suspension, the sterile inhalation suspension containing the PDE-4 or PDE-3 / 4 inhibitor has high bioavailability, good patient medication compliance and good preparation stability.

The invention relates to injectable interlocking cross-linked hyaluronic acid or hydrogel thereof, a preparation method and application. The preparation process of the hydrogel comprises a two-step cross-linking step of hyaluronic acid or salt thereof and a cross-linking agent endogenous polyamine and a dialysis step. In the two steps of crosslinking, the first step of crosslinking forms a net-shaped structure, and the second step of crosslinking further forms an interlocking structure. The hydrogel prepared through the technology is subjected to moist heat sterilization, high in degradation resistance, excellent in viscosity, good in supporting performance, easy to push and inject, high in safety and easy to redissolve, the clinical requirements for injection products are met, the whole technology is easy, convenient and rapid to operate, and industrialization is easy to achieve.

The invention belongs to the field of medicine, and particularly relates to compound earthworm capsule traditional Chinese medicine extract powder and a preparation method thereof.The preparation method comprises the following steps that 280 parts by weight of ligusticum wallichii, 240 parts by weight of astragalus membranaceus and 160 parts by weight of achyranthes bidentata are prepared, and the preparation method of the traditional Chinese medicine extract powder comprises the steps that 160 parts by weight of astragalus membranaceus is taken to be laid on a tray, subjected to 121 DEG C moist heat sterilization in a traditional Chinese medicine sterilization cabinet for 30-60 min, dried for 2-4 h and smashed to be 60 meshes for use; adding 70% ethanol which is 3 times of the total weight of the extracted medicinal materials, independently soaking the ligusticum wallichii, and continuously soaking the radix achyranthis bidentatae and 1 / 3 of the astragalus membranaceus; adding 70% ethanol which is 3 times of the total weight of the extracted medicinal materials, decocting and refluxing for 2 hours, firstly concentrating the secondary decoction, and then concentrating the primary decoction until the specific gravity of the extract is 1.1-1.2; adding radix astragali powder, mixing, carrying out vacuum belt type drying, and crushing with a 60-mesh sieve; compared with an existing preparation method of the compound earthworm capsule traditional Chinese medicine extract powder, the preparation method has the advantages that the production period is greatly shortened, the content retention rate is improved to the maximum extent, the product quality is guaranteed, and the whole process is easier to apply and produce and is easier to popularize.

The invention relates to the technical field of membrane separation, in particular to a preparation method of a steam-sterilization-resistant hollow fiber membrane component, which is used for preparing a hollow fiber membrane capable of resisting moist heat steam sterilization at 121 DEG C, and the membrane can resist moist heat sterilization under the pressure of 2bar. The preparation method comprises the following steps: bundling the hollow fiber membrane filaments and loading the hollow fiber membrane filaments into a membrane shell, selecting a steam-resistant sterilization type hollow fiber membrane as the hollow fiber membrane filaments, selecting a polysulfone material as the membrane shell material, and then preparing the hollow fiber membrane module by using epoxy resin pouring sealant through centrifugal casting, and the module can be applied to the field of medicine. According to the preparation method of the steam-sterilization-resistant hollow fiber membrane module, the epoxy resin pouring sealant is subjected to the steps of centrifugal pouring, hollow fiber membrane module assembling treatment and hollow fiber membrane module post-treatment, so that high-temperature steam sterilization treatment can be carried out in an environment of 121 DEG C, and the requirements of cell perfusion culture and virus removal are met; therefore, the pharmaceutical process is more effectively applied.

The invention belongs to the technical field of agriculture, and provides an artificial feed and a method for testing the influence of the artificial feed on the growth and development of tomato leaf miners, the method comprises three artificial feed formulas, and standardized feed blocks are prepared through moist heat sterilization and low-temperature preservation. The method comprises the following steps: subpackaging feed by adopting finger-shaped tubes, inoculating according to the density of 3 larvae in each tube, feeding at 25 + / -1 DEG C, and measuring indexes such as the duration, the survival rate, the pupation rate and the eclosion rate of the larvae. Results show that the difference between the formula I and natural foodstuff is minimum, the larva survival rate, pupation rate, pupa weight, eclosion rate and hatching rate are not obviously different from those of natural tomato leaf feeding, and although the larva and pupa duration is prolonged and the single female egg laying amount is reduced, the complete life history of tomato leaf miners can still be supported. According to the method, indoor stable test insects with consistent physiological status are realized, and reliable insect source guarantee is provided for biological research and prevention and control technology development of the tomato leaf miner.

This application provides a sodium hyaluronate gel and its preparation method, belonging to the field of biomedical materials, aiming to solve the problem of severe performance degradation of cross-linked sodium hyaluronate gels during moist heat sterilization. The gel contains cross-linked sodium hyaluronate and is compounded with a solvation shell-building agent and a high-temperature free radical scavenger. These two agents synergistically constitute a dual protection system, jointly protecting the gel structure. The preparation method includes matrix cross-linking, in-situ loading of the protection system through dialysis equilibrium, homogenization, and terminal moist heat sterilization. After moist heat sterilization at 121°C, the gel of this invention exhibits a high storage modulus retention rate, demonstrating excellent thermal stability and synergistic effects, ensuring stable product performance.

The invention relates to the technical field of biomedical materials, in particular to a preparation method of a hydroxyapatite microsphere-containing composite solution for injection, which comprises the following steps: S1, adding a high-molecular polymer, a local anesthetic and nutritional ingredient dry powder into a buffer salt solution according to a certain proportion, and stirring until complete dissolution; s2, adding the hydroxyapatite microspheres into the non-crosslinking composite solution, and blending until the hydroxyapatite microspheres are uniformly dispersed; and S3, filling, centrifugally defoaming and carrying out moist heat sterilization to obtain the hydroxyapatite-containing composite solution for injection. The composite solution containing hydroxyapatite microspheres for injection is prepared by the preparation method. The composite solvent is applied to the fields of wrinkle filling and repairing and tissue engineering materials. The hydroxyapatite microsphere prepared by the method does not contain chemical cross-linking agent residues, is smooth in pushing, and can greatly improve the solid content of the hydroxyapatite microsphere, so that the safety, experience and repair effect of injection filling are improved.

The present disclosure provides a gel material, its preparation method and use. The gel material is obtained by crosslinking endogenous polyamines with hyaluronic acid, the endogenous polyamines include spermine and / or spermidine, and the crosslinking between endogenous polyamines and hyaluronic acid includes two-site crosslinking, three-site crosslinking or four-site crosslinking. The reactive crosslinking sites can be controlled to affect various performances of the gel, as well as the degradation and release rate of polyamines. The gel has a low elastic modulus loss rate after moist heat sterilization, and can effectively maintain the rheological performance of the gel before sterilization, greatly improving the thermal stability of the hyaluronic acid gel, and also improving the usability of the gel in fields such as soft tissue filling, soft tissue repair and medical beauty.

The application discloses a composite solution, a preparation method and application thereof. The composite solution comprises a hyaluronic acid substance, PN, a phosphate buffer and water; wherein the weight average molecular weight of the hyaluronic acid substance is 30-100 WD a; the mass ratio of the PN and the hyaluronic acid substance is (1.5-20):1; and the concentration of alkali metal cations provided by the phosphate buffer in the composite solution is 0.09-0.22 mol / L. The composite solution prepared by the application is gelatinous after moist heat sterilization, has higher viscoelasticity and higher macromolecular chain entanglement degree compared with a low-viscosity water-light product of a partial fluid, can delay the degradation rate of the hyaluronic acid enzyme in the body and prolong the retention time in the body, has stronger cohesion, has excellent anti-migration and anti-dispersion capacity after injection and is not easy to diffuse to surrounding tissues. Meanwhile, the composite solution has the functions of PN and HA, realizes multiple actions of the water-light product, is helpful to reduce injection frequency and improve treatment experience.

The invention relates to the technical field of medicinal material sterilization, and discloses a sterilization and disinfection device for primary processing of traditional Chinese medicinal materials, which comprises a sterilization box, and also comprises a sealing cover mounted at the top of the sterilization box, water is filled in the sterilization box, and the sealing cover is used for heating the water to sterilize the traditional Chinese medicinal materials in a moist heat manner; the medicinal material net is arranged in the sterilization box and is used for placing traditional Chinese medicinal materials; the lifting mechanism is arranged in the sterilization box and used for turning over the medicinal material net to release the traditional Chinese medicinal materials to be separated from the medicinal material net; and the cleaning mechanism is arranged in the sterilization box and is positioned below the medicinal material net. Water in the sterilization box is heated to generate water vapor, a high-temperature and high-humidity environment is formed, microorganisms on the surfaces and in traditional Chinese medicinal materials can be effectively killed, the sterilization effect is remarkably improved, in the sterilization process, the lifting mechanism drives the medicinal material net to move up and down, and the contact area of the traditional Chinese medicinal materials and the water vapor is increased; and the uniformity and thoroughness of sterilization are further improved.

A tray laying machine for moist heat sterilization of medicine powder comprises a rack, and a sterilization tray placing device, a sterilization tray conveying device and a charging tray laying device are arranged on the rack. Automatic medicine powder charging and tray laying are achieved through the sterilization tray containing device, the sterilization tray conveying device and the charging and tray laying device, closed conveying and accurate operation are achieved through automatic connection, the links of temporary medicine powder storage and manual scooping are omitted, the medicine powder splashing loss and the microbial contamination risk are remarkably reduced, meanwhile, the charging uniformity and the tray laying efficiency are improved, and the labor intensity of workers is lowered. The labor cost and the labor intensity are reduced, and the large-scale production requirement is met.

The present invention relates to the technical field of polysaccharide material modification, and specifically relates to a high-viscoelastic crosslinked biopolysaccharide gel, a preparation method therefor, and an application thereof. After a carboxyl-group-containing biopolysaccharide and a diaminodiketopiperazine-based crosslinking agent are dissolved and mixed uniformly, a crosslinking reaction is generated under the action of a condensing agent to obtain a high-viscoelastic crosslinked biopolysaccharide gel; following moist heat sterilization, the resulting high-viscoelastic crosslinked biopolysaccharide gel meets conditions for the application thereof, and can be applied to the preparation of injectable formulations, internal filler formulations, or topical pharmaceutical carrier formulations. The crosslinked biopolysaccharide gel prepared according to the present invention exhibits large headroom, in terms of viscoelasticity data, to accommodate moist heat sterilization, which ensures product sterility while guaranteeing the product has good viscoelastic performance, with low cytotoxicity, high product purity, and long service life.

The invention belongs to the field of pharmaceutics, and particularly relates to a composition containing budesonide and formoterol, and the composition provided by the invention is in a specific pH range, adopts a specific surfactant addition mode, also adopts moist heat sterilization, and is free of toxic and side effects. The prepared inhalation suspension of formoterol and budesonide has better delivery rate and total delivery amount, better fine particle fraction and smaller particle size distribution, has the advantages of low temperature, short time, strong penetrating power, economy, rapidness and the like compared with dry heat sterilization and moist heat sterilization, and is more suitable for industrial mass production.

The present application relates to the technical field of microorganism or culture thereof processing, and particularly relates to a method for breeding pleurotus ostreatus mycelium by using highland barley as a substrate. The method is as follows: highland barley is crushed and sieved, then water is added to prepare highland barley slurry, and a complex enzyme system composed of amylase and cellulase is added to perform liquid enzymolysis to obtain an enzymolysis substrate; lactic acid bacteria strains are inoculated into the enzymolysis substrate to perform solid-state fermentation to obtain a fermentation substrate; the fermentation substrate is subjected to moist heat sterilization treatment to obtain a sterilized substrate; the sterilized substrate is inoculated with pleurotus ostreatus original seeds, and is placed in a culture environment for culture to obtain pleurotus ostreatus mycelium. The scheme breaks the physical barrier of the fiber network of the highland barley epidermis and the starch crystal structure of the endosperm by using the complex enzyme system and lactic acid bacteria fermentation, and the substrate is pre-degraded before the mycelium is inoculated, so that the mycelium can directly absorb free carbon sources, the germination time is shortened, the daily growth rate is improved, the residual sugar rate of the substrate is reduced, and the dry weight biomass of the mycelium is increased.