227 results about "Moist heat sterilization" patented technology

The invention relates to a biological organic foliar fertilizer and a preparation method thereof. The biological organic foliar fertilizer is obtained by adding 50 parts weight of deep well water in 50 parts by weight of waste such as soybean residues, crushed rapeseed meal and the like; mixing the above materials after moist heat sterilization; inoculating compound microbial strains; fermenting for 30 days at a constant temperature; removing fermentation residues, taking the fermentation broth for use; putting 48 parts of the fermentation broth, 15 parts of fulvic acid, 5 parts of magnesium element, 5 parts of manganese element, 5 parts of ferrum element, 5 parts of zinc element, 2 parts of copper element, 2 parts of molybdenum element, 3 parts of a surfactant, 2 parts of PCPA , 2 parts of NAA and 6 parts of sterile water in a reaction kettle; mixing the above materials uniformly; heating to a temperature of 45 DEC C; keeping the temperature for 48 hours for reaction; and adjusting a pH value to 3-4. The biological organic foliar fertilizer can promote crop photosynthesis, activate activities of various enzymes to promote the growth, increase disease prevention and antibiosis of plants and increase resistance of the plants. The biological organic foliar fertilizer is pollution-free and harmless, has durable fertilizer efficiency, can strengthen seedlings, prevent diseases, improve soil and increase production, has no residues after use, improves quality of agricultural products, makes products appear on the market 7-15 days earlier, and increases agricultural economy benefits.

The invention discloses a method for producing a golden flower dark tea by employing rapid fermentation of a raw dark tea brick. The method comprises the following steps: (1) sterilizing in a fermentation room, sterilizing an air-tight fertilization room with an air inlet and an air outlet; (2) sterilizing the dark tea, carrying out moist heat sterilization on a raw dark brick tea in an autoclaved sterilization pot; (3) artificially inoculating an eurotium cristatum strain and blending to prepare a strain solution, cooling the sterilized raw dark tea brick to a room temperature, and soaking in the strain solution or evenly spraying the strain solution on the surface of the tea brick; and (4) uniformly and vertically putting the inoculated tea brick on a fermentation frame of a sterilized fermentation room, fermenting for 7-21 days, taking out and carrying out reduced pressure drying, so as to obtain the golden flower dark brick tea brick. According to the method disclosed by the invention, efficient flowering of the dark tea is achieved; the cost is low; each gram of dry tea contains not more than 1.8*10<7> eurotium cristatum in average; the soup brewed by the golden flower dark tea is clear, and fragrant in aroma; the produced brick tea is stable in quality, can be stored for a long period of time, and has relatively good nutrients and relatively high collection value.

The invention relates to a preparation method of glycerin and fructose injection, comprising the following steps: adopting a buffer solution to regulate the pH value of glycerol and fructose aqueous solution to be 4.0 to 5.5, carrying out moist heat sterilization at the temperature of 115 DEG C for 30 minutes or at the temperature of 121 DEG C for 10 minutes after filling, and then cooling. The pHvalue of the glycerin and fructose injection prepared by the preparation method is stable, and the content of 5-HMF is low. The invention adopts the sterilization process of a quick cooling sterilizer, thereby shortening the heating time during the sterilization process and reducing the degradation of fructose.

The invention discloses a fat emulsion injection containing nimodipine and a preparation method thereof. The fat emulsion injection containing the nimodipine is prepared from nimodipine, injection oil, injection phospholipid, injection polyethylene glycol hydroxy stearate, an isoosmotic adjusting agent, a pH regulator and injection water. The preparation method comprises the following steps: mixing the nimodipine, the injection oil, the injection phospholipid with the injection polyethylene glycol hydroxy stearate, and agitating to clear and transparent as an oil phase; evenly mixing the isoosmotic adjusting agent with the injection water as a water phase; mixing the oil phase with the water phase; processing emulsion particles into the stated particle size range by adopting a two-step homogenization method; bulking and sterilizing after adjusting the pH value. The injection phospholipid and the polyethylene glycol hydroxy stearate are adopted as a mixed emulsifier, and the prepared fat emulsion injection containing the nimodipine can endure high-pressure moist heat sterilization, and does not need an expensive sterile manufacturing process, so that the production cost is greatly reduced, and industrial production is facilitated.

The invention relates to a traditional Chinese medicine preparation-Liuwei Anxiao Pills and a preparing method of the traditional Chinese medicine preparation, belonging to the field of traditional Chinese medicines. The traditional Chinese medicine preparation is characterized by comprising the following components in weight percent: 40-55 parts of Tibet inula, 160-200 parts of rhubarb, 80-100 parts of Kaempferia galangal, 200-250 parts of Gypsum Gypsum Rubrum, 110-150 parts of myrobalan and 250-300 parts of Tronae. In the preparing method, on the basis of the traditional process, the original single crushing pattern is changed, a sterilization process is changed, i.e. the original moist heat sterilization process for medicine powder produced after medicinal slices are crushed is changed into a cobalt 60 irradiation process, and the pattern of collecting and coating volatile oil is changed. The preparing method sufficiently ensures that volatile components in the Tibet inula are not lost and oily components in the Tibet inula are not lost. In addition, the drug effect is more effectively ensured, the product name quality is ensured, and benefits of patients are guaranteed.

The invention relates to a botanical fungicide and the preparation method thereof, which relate to a fungicide and the preparation method. The invention solves the shortcomings of the existing fungicide in severe environmental pollution and resistance due to long-term use. The botanical fungicide of the invention is made from stellera chamaejasme, liquorice, aconite and radix sophorae falvescentis. The method of the invention comprises the steps of: 1. weighing and mixing the stellera chamaejasme, liquorice, aconite and radix sophorae falvescentis; 2. leaching the ethanol; 3. filtering; 4. distilling; 5. moist heat sterilization. The botanical fungicide of the invention has the advantages of innocuity, low residuals, small dosage, no pollution, no resistance, strong bacteriostatic activity, high selectivity, diversified raw material sources, easy match with other biological pesticides, etc. The method of the invention has the advantages of simple process, and low cost. Compared with the chemical agent, the Chinese herbal medicine fungicide has the advantages of many active sites, long agent duration, high degradation speed, small pollutions to the environments, etc.

The invention discloses a collagen dermal implant and a preparation method thereof. The method comprises the following steps of dissolving collagen in ultrapure water; performing refrigeration; adding a chemical cross-linking agent; performing uniform stirring at a low temperature; performing refrigeration; performing heating in a water bath to start a cross-linking reaction, so as to prepare cross-linked collagen gel ;adding normal saline-ammonia water into the cross-linked collagen gel, and performing a blocking reaction; adding normal saline into the cross-linked collagen gel after the blocking reaction for purification; granulating the purified cross-linked collagen gel by a screening method; and dispersing the granulated cross-linked collagen gel particles in normal saline to prepare a cross-linked collagen suspension; filling the cross-linked collagen suspension into a pre-filling injector; and performing moist heat sterilization to prepare the collagen dermal implant. The collagen obtained through fermentation has a physical filling function through the cross-linking reaction, and in addition, the collagen dermal implant is free of immunotoxic reaction, has good biocompatibility and can be used as a body tissue filler in medical cosmetology.

The invention discloses a tissue culture method of dendrobium stem seedlings. The method comprises serial steps of building an intermittent immersion solution culture system, wherein the steps comprise a first step of preparing and sterilizing an MS culture solution, wherein components and use amounts thereof of the MS culture solution are as follows: MS, 0.5 to 5.0 mg / L of NAA, 50 to 200 g / L of potato juice and 30 g / L of saccharose; adjusting a pH value to be 5.70 to 6.00; after the MS culture solution is prepared, placing the MS culture solution into a reagent bottle; transferring the reagent bottle into a moist heat high-pressure sterilization pot; sterilizing the reagent bottle by using a moist heat sterilization method for future use; a second step of sterilizing an intermittent immersion type bioreactor by using the moist heat sterilization method; a third step of inoculating explants into the intermittent immersion type bioreactor containing the MS culture solution; a fourth step of setting the relative parameters of the intermittent immersion type bioreactor. The method disclosed by the invention is used for breeding the dendrobium stem seedlings, and has the beneficial technical effects of high proliferation rate, high degree of automation, small amount of labor, good tissue culture seedling quality and high adaptability.

A preparation method for an adsorbent capable of removing heavy metals such as Cu and Pb comprises: dropwise adding a 4M NaOH solution into a selective medium solution to adjust pH of the medium to 6-8; inoculating 2% (V / V) manganese oxidating bacteria pseudomonas sp.G7 strain to the medium solution which is subjected to heat sterilization with steam for 30 min at 115 DEG C, then adding a filtered disinfected buffer solution to again adjusting pH of the solution to 6-8; performing concussion culture for 12-24 h at 20-30 DEG C, adding filtered disinfected MnCl2 (to make the final concentration at 1 mM); continuously culturing for 1-3 weeks at 20-30 DEG C to aggregate a large amount of dark brown even black manganese oxides on the surface of bacterium colonies; and centrifuging with centrifugal force of 7500 g for 10-20 min to remove the medium, washing with a phosphatic buffer solution for 3-5 times, and finally dissolving surface adsorbed Mn<2+> with a 20-80 mM CuSO4 buffer solution to obtain biological manganese oxides.