The invention discloses a cucurbit and vegetable breeding substrate. A production method of the cucurbit and vegetable breeding substrate comprises steps of (1) mixing grass carbon, fermented biogas residues, bio-organic fertilizers and the like; (2) adding ammonium sulphate, potassium sulphate, monoammonium phosphate, manganous sulfate, boric acid and the like to a sprayer containing water and stirring uniformly fully for use; and (3) sieving the bio-organic fertilizers in the step (1) and enabling the bio-organic fertilizers to sink into other materials of the step (1) except the bio-organic fertilizers, adding the mixture solution of step (2) into the mixture of the step (1), turning and mixing once, using a square-hole screen to sieve once till all mixtures pass the screen, adding proper water, continuing to turn and stir three times, using a plastic film to cover mixtures for stack and smothering for 48 hours after completion of preparation, sampling, and performing quantitative package after inspection. The substrate has good fertilizer effects, releases nutrition slowly and is disease resistant, degeneration resistant, comprehensive in nutrition and good in pertinency. The germinate rate and seedling emergence rate of seeds are improved, seedlings are strong and free from excessive growth, soaked roots, dwarf and mummification, blossom buds of seedlings are differentiated normally, and characteristics, the yield and the quality of agricultural products are guaranteed.