Manufacturing method for morchella mother culture
A preparation method, the technology of Morchella are applied in directions such as botanical equipment and methods, horticulture, application, etc., to achieve the effects of promoting domestication and cultivation, more bikaryotic mycelium, and strong kink force
Inactive Publication Date: 2011-01-19
云南省农业科学院高山经济植物研究所
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Problems solved by technology
[0003] The object of the present invention is to solve the problems in the above-mentioned existing hickory chick female seed production method, and provide a method for making a hickory chick female seed that is strong in hyphae, strong in kink strength and easy to form sclerotia on the original species or cultivated species
Method used
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Effect test
Embodiment
[0011] 1. Select 200g of fresh non-insect-eaten poplar branches and cut them into 1-2cm pieces or crush 100g of the original dry leaves of poplar, add them to 1L of water and boil for 25-30min, filter and extract the juice to obtain poplar extract.
[0012] 2. After mixing Populus roundleaf extract and PDA culture solution at a mass ratio of 1:1, add Na 3 PO 4 12H 2 O0.2%, KH 2 PO 4 0.2%, MgSO 4 0.05%, V B1 0.005%, stir evenly and sterilize at 121±1°C for 30 minutes to make culture medium.
[0013] 3. After the culture medium is cooled, pick the mother species of hickory chick, cultivate the culture medium for 11 days at a constant temperature of 23°C, and then put it in an incubator at 10°C for 5 days, and the mother species of hickory chick is completed.
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The invention provides a manufacturing method for morchella mother culture. The method is characterized by comprising the following steps of: 1, boiling 1 to 3 parts of fresh insect-free populus bonatii smashed branches or populus bonatii leaves in 10 parts of water based on a mass ratio for 25 to 30 minutes, filtering the mixture, and taking juice to prepare populus bonatii leachate; 2, mixing the populus bonatii leachate and PDA culture solution in a mass ratio of 1: 1, adding 0.2 percent of Na3PO4.12H2O, 0.2 percent of KH2PO4, 0.05 percent of MgSO4 and 0.005 percent of VB1 based on the mass percent of the mixed liquor, uniformly stirring the mixture, and sterilizing the mixture at the temperature of 121 +/1 DEG C for 30 minutes to prepare a culture medium; and 3, cooling the culture medium, inoculating the morchella mother culture, culturing the inoculated culture medium at the constant temperature of 23 DEG C for 10 to 12 days, and culturing the culture medium in an incubator at the temperature of between 8 and 12 DEG C for 4 to 6 days. The manufacturing method has the advantages of strong hypha, strong twisting force, and easy formation of sclerotium on protospecies or cultivar.
Description
Technical field: [0001] The invention relates to a method for preparing mother species of hickory chick. Background technique: [0002] Morchella is a kind of rare food (medicine) fungus, which has begun bionic domestication and cultivation. The production of mother species of morel is the first step in bionic domestication and cultivation, and it is also a key link. At present, the mother species of hickory chick generally uses PDA medium as the mother culture medium. The mycelium of this mother culture medium shows the characteristics of slender mycelia and weak kink strength on test tubes or petri dishes. The original species or cultivar mycelia made by the culture medium are also slender and difficult to form sclerotia, resulting in relatively low yield. Therefore, researching a kind of high-quality mother seed production method that is strong in mother seed, strong in kink strength and can form sclerotia on original seed or cultivated species has a significant effect ...
Claims
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IPC IPC(8): A01G1/04
Inventor 侯志江程远辉戚淑威徐中志和琼姬赵琪王泽清
Owner 云南省农业科学院高山经济植物研究所
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