241 results about "Morchella" patented technology

The invention discloses an intelligent nutrient circulation regulation and control method for a morchella esculenta fruit forest intercropping system based on the Internet of Things, particularly relates to the technical field of intelligent agriculture, and is used for solving the problem of symbiosis imbalance of hyphae and fruit trees caused by nutrient regulation and control lag and static threshold setting in the prior art. Hypha density, electric signal dominant frequency and root system absorption rate data are collected in real time through a multispectral impedance sensor and an ion selective electrode array, and a space-time aligned dynamic matrix is constructed; dynamically updating a nutrient balance threshold value based on a real-time ratio of a hypha metabolism rate to an absorption rate, and generating a nutrient transfer priority by combining collaborative analysis of a hypha transfer direction included angle and an electric signal main frequency offset gradient; a continuous transfer path is identified through a region growing algorithm, water and fertilizer amounts are distributed according to priorities, and a local area instruction is generated; and inverting the equipment response delay by using the high-frequency pulse signal, synchronously adjusting the execution frequency and updating the dynamic matrix and the threshold parameter, thereby realizing the dynamic adaptation and autonomous optimization of the nutrient supply and demand of the intercropping system.

The invention belongs to the technical field of morchella esculenta planting, and particularly relates to a non-grain-based morchella esculenta nutrient medium, a nutrient bag, a preparation method and a morchella esculenta planting method. The nutrient medium can be prepared by taking cassava as a main material and rice husks and mushroom dregs as auxiliary materials, controlling the amylopectin content and the aeration porosity within a preset range by adjusting the ratio of the raw materials, and adding quick lime, gypsum and the like as pH value regulators. The problem that a traditional nutrition bag depends on grain resources such as wheat is solved, and resource recycling is achieved through cassava and agricultural waste mushroom dregs and rice husks. Particularly, under the condition of optimal proportioning, the fruiting amount of the morchella in unit area is obviously increased compared with that of a traditional wheat substrate, and the raw material cost is reduced. Moreover, the key influence of the synergy of the amylopectin content and the aeration porosity in the non-grain-based morchella esculenta nutrition medium on morchella esculenta fruiting is found, and a reference direction is provided for screening of the non-grain-based morchella esculenta nutrition medium.

The invention belongs to the technical field of microorganisms, and particularly discloses a morchella growth-promoting strain bacillus MSB09, which is classified and named as bacillus sp. And has a preservation number of GDMCC No: 65938. Morchella is used as a precious edible and medicinal fungus, the planting scale is continuously enlarged, but the soil continuous cropping problem has become a main obstacle for soil-covered cultivation and fruiting of the morchella, the soil microorganism structure of the morchella is studied, and the morchella can be used for promoting growth of the morchella. And beneficial microorganism bacillus (Bacillus sp.) MSB09 is screened out, so that technical support is provided for reconstruction of soil micro-ecology, alleviation of continuous cropping obstacles of morchella esculenta and biological prevention and control.

The invention discloses a bionic gastrodia elata cultivation method based on modular mushroom sticks and an ecological prevention and control system, and belongs to the technical field of ecological cultivation of traditional Chinese medicinal materials. The cultivation method specifically comprises the following steps: (1) adopting modular mushroom sticks; (2) adopting a pest and disease damage prevention and control method; (3) adopting an ecological cooperation and environment adaptation cyclic utilization method; (4) an intelligent and automatic production method is adopted; (5) adopting a bionic cultivation process; and (6) adopting a gastrodia elata-morchella esculenta rotation method. According to the method, the wood cost is reduced by 80%, the disease occurrence rate is smaller than or equal to 3.5%, the insect attack rate is smaller than or equal to 4%, the chemical pesticide use amount is reduced by 90%, the yield of gastrodia elata per mu reaches 450 kg, the yield is increased by 20%, the yield of fresh mushrooms per mu is 310 kg through rotation of morchella esculenta, the comprehensive income is increased by 6000 yuan / mu, and the method is suitable for large-scale planting of resource-saving gastrodia elata in plains and under-forest planting scenes at the altitude of 800-1200 m.

The invention provides a high-yield cultivation method for rice-morchella esculenta crop rotation, which specifically comprises the following steps: step 1, remaining previous rice stubbles, and turning over the rice stubbles into soil; step 2, laying a fungus bed substrate on a furrow surface and sowing; step 3, spraying a bacterium-promoting nutrient solution and a protective agent; step 4, after the morchella esculenta is harvested, turning over the fungus bed substrate into soil and applying a base fertilizer; 5, rice is transplanted, and tillering fertilizer, fish protein fermentation liquor and leaf fertilizer are applied; step 6, spraying an insecticide; step 7, harvesting the rice and remaining stubbles, and continuing planting the morchella esculenta; according to the method, the problems of nutrition imbalance, chemical residues, fertilizer efficiency lag and the like in the existing crop rotation technology are solved one by one through the steps of systematic optimization of a fungus bed substrate, nutrition management, base fertilizer preparation and the like.

The invention discloses a chelerythrine-containing compound microbial agent and application thereof, and belongs to the technical field of biological control. The invention provides a chelerythrine-containing compound microbial agent to solve the technical problems that in the prior art, only a single microbial agent and a plant extract are used, the biological control effect is poor, the stability is low, and the target spot is single. The compound microbial agent is prepared from a mortierella alpina extract, a trichoderma hairy extract and chelerythrine according to a specific compounding ratio. The compound microbial agent provided by the invention can delay the development of drug resistance of pathogenic bacteria through a multi-target effect, has better stability and stronger control effect, reduces the use amount of chemical pesticides, reduces the harm to the environment and human body, can be applied to tobacco planting production, can effectively reduce the control cost of tobacco black shank, and has broad application prospects. The economic benefit of tobacco planting is improved.

The invention belongs to the technical field of microorganisms, and particularly discloses a morchella growth-promoting strain Clonostachys roseMSF05, the classification name is Clonostachys rosea, and the preservation number is GDMCC No: 65937, morchella is used as a precious edible and medicinal fungus, the planting scale is continuously enlarged, but the soil continuous cropping problem has become a main obstacle for soil-covered cultivation and fruiting of the morchella, the soil microorganism structure of the morchella is studied, and the morchella growth-promoting strain CClonostachys roseMSF05 can be used for promoting growth of the morchella. And the beneficial microbial strain clonostachys rosea MSF05 is screened out, so that technical support is provided for rebuilding soil micro-ecology, relieving continuous cropping obstacles of morchella esculenta and biological prevention and control.

A cultivation method for Morchella on patterned layer frames is provided. The method includes: (1) preparing and laying a culture substrate, and sowing; (2) supplementing an exogenous nutrient material; (3) mycelium culture; (4) primordium induction; (5) primordium differentiation; (6) management of a young mushroom period; (7) maturity management; and (8) erchao mushroom management. The method adopts a low temperature of (5-9)° C. to promote a transformation of an exogenous nutrition and adopts a low temperature of (2-4)° C. to induce a differentiation of primordium and other measures, to address a bottleneck of industrial development that cannot continuously supply fresh Morchella to the market, which is caused by some problems in the cultivation of Morchella, such as an unstable yield and seasonal limitation in the existing cultivation of Morchella. The method can carry out industrial facility cultivation for Morchella, and can obtain Morchella with stable yield and good quality.

The invention discloses a morchella esculenta extract as well as an extraction method and application thereof, and belongs to the technical field of plant medicine product separation. The invention relates to a specific extraction method of phenolic acid of morchella, through the synergistic effect of low-temperature plasma pretreatment, targeted enzymolysis and deep eutectic solvent extraction, the extraction rate of phenolic acid is remarkably improved, particularly, specific extraction of chlorogenic acid can be realized, and the purity of chlorogenic acid is greater than 85%. The process is environment-friendly and efficient, and the obtained product has important application value in the fields of inflammation resistance and oxidation resistance.

The invention aims to provide a morchella esculenta breeding method, an obtained morchella esculenta strain and application, the morchella esculenta is named as Morchella sextella NYD1, the taxonomic name of the morchella esculenta is Morchella sextella NYD1, the morchella esculenta is preserved in Guangdong Microbial Culture Collection Center, the preservation number of the morchella esculenta is GDMCC No: 66082, and the preservation date of the morchella esculenta is April 21, 2025. The morchella strain is further utilized as a mother strain to carry out domestication and cultivation, the morchella with the fruiting yield up to 400 kg / mu or above, excellent agronomic characters and rich nutritional ingredients such as crude protein, total sugar, iron, zinc and calcium is finally obtained, the content of the crude protein is not lower than 23 g / 100 g, the content of the total sugar is not lower than 55 g / 100 g, the content of the iron is not lower than 240 mg / kg, the content of the zinc is not lower than 70 mg / kg, and the content of the calcium is not lower than 76 mg / kg.

The utility model relates to the technical field of morchella esculenta harvesting equipment, and provides a morchella esculenta harvesting device which comprises a bottom frame and a grip, the grip is arranged on the bottom frame, an extension assembly is arranged between the bottom frame and the grip, a bottom cavity is formed in the bottom frame, a grabbing assembly is arranged on the bottom frame, and the grabbing assembly comprises a first telescopic rod. The first telescopic rods are installed in the bottom cavities, movable frames are rotationally connected to the two sides of the bottom frame through pin shafts, long holes are formed in the side surfaces of the movable frames, and grabbing frames are movably connected into the side surfaces of the movable frames in a sleeved mode. By means of the technical scheme, the problems that in an existing harvesting mode in the related technology, generally, a cutter needs to be manually taken for completion, the mode needs one hand to take morchella esculenta and one hand to cut the morchella esculenta, consequently, the morchella esculenta needs to be manually and continuously squat and stand up, the harvesting efficiency is low, and the labor intensity is high are solved. And meanwhile, the consumption of manual physical power is relatively large.

The invention relates to the technical field of morchella esculenta planting, in particular to a film mulching device for morchella esculenta planting. One side of the outer wall of the shell is rotationally connected with a driving rod and a driven rod, and one end of the outer wall of each of the driving rod and the driven rod penetrates through the shell; traveling wheels are fixedly connected to the two ends of the outer wall of the driving rod and the two ends of the outer wall of the driven rod; a film covering mechanism is arranged on the inner side wall of the shell; a motor is fixedly connected to one side of the inner wall of the shell; a rotating shaft is arranged at the output end of the motor; the rotary shaft drives the first bevel gear to rotate, the first bevel gear drives the second bevel gear to rotate, the second bevel gear drives the first reciprocating rotary rod to rotate, the first reciprocating rotary rod drives the reciprocating plate to reciprocate, the reciprocating plate drives the punching plate and the punching rod to reciprocate up and down, and the punching rod conducts punching operation; the device performs film laying and punching at the same time, and the film laying and the punching are performed synchronously, so that the film covering time of the morchella is saved, and the film covering efficiency of the morchella is improved.

The invention discloses a morchella seeding and soil covering machine device which comprises an inclined frame, a driving assembly and a shell, a fixed frame is mounted at the bottom end of a frame plate, and a seed storage box, a soil storage box and a driving motor are arranged in the shell, and the morchella seeding and soil covering machine device is characterized in that a rectangular pipeline is arranged in the middle of the seed storage box, and an inclined channel is mounted at the bottom of the rectangular pipeline; a conveying cavity is formed in the bottom of the soil storage box, a stirring shaft is installed in the conveying cavity, and the conveying cavity penetrates through the frame plate. The linkage mechanism based on the driving motor is designed, the conveying cavity and the vibration assembly are driven to act, covering soil is output from the conveying cavity to the lower inclined frame, the covering soil is dispersed by the vibration assembly, even stirring can be achieved when the covering soil is discharged, covering soil particles are dispersed, and the covering soil thickness of morchella is closer.

The invention discloses an application of morchella enzymolysis polysaccharide in regulating lipid metabolism disorder. The morchella enzymolysis polysaccharide disclosed by the invention can be used for regulating lipid metabolism disorder, regulating intestinal flora and preventing liver injury, oxidative stress and inflammation. The morchella esculenta adopted by the invention is medicinal and edible morchella esculenta, is free of chemical modification and is high in safety; and the enzymolysis process is low in cost, easy to scale and suitable for functional food or medicine development. In addition, the invention provides the application value of 8-aminooctanoic acid as a lipid metabolism disorder marker for the first time, and expands the application scene of EMIP in preparation of diversified products for regulating intestinal flora, improving liver injury, reducing oxidative stress and the like.

The invention discloses a method for efficiently reducing nitrogen and phosphorus in greenhouse vegetable soil based on morchella esculenta cultivation, and belongs to the technical field of soil improvement and edible fungus cultivation. In order to solve the problem of soil nitrogen and phosphorus eutrophication caused by long-term continuous cropping of a facility vegetable base, by screening a specific Morchella sextella strain and combining with optimized strain culture, inoculation and field management technologies, the absorption efficiency of the Morchella sextella on the soil nitrogen and phosphorus is remarkably improved. The method specifically comprises the following steps: soil nutrient detection and eutrophication evaluation, directional culture of strains, layered soil covering and sowing, accurate supplementation of exogenous nutrition bags, and dynamic monitoring of hypha growth and nutrient absorption. Experiments show that the content of total nitrogen (TN) in soil can be reduced by 11.27%-18.01%, the content of total phosphorus (TP) can be reduced by 2.72%-6.16%, the reduction amplitude of rapidly available phosphorus (AP) can reach 2.12%-11.35%, and the accumulation of nitrogen and phosphorus in the soil can be effectively relieved. According to the method, a dual absorption mechanism is formed through hypha metabolism and sporocarp, rapid reduction of greenhouse vegetable soil nutrients is achieved, and a new way is provided for sustainable development of greenhouse agriculture.

The invention discloses an intelligent temperature and humidity control system for factory planting of morchella esculenta, relates to the technical field of intelligent regulation and control, and aims to solve the problem of yield reduction caused by influence difference of morchella esculenta on temperature and humidity interaction in different stages. Obtaining a standard temperature value and a standard humidity value of each growth stage of the morchella, counting the area ratio of the morchella in different growth stages in each divided region, and detecting the growth speed and the death ratio of the morchella in different growth stages in each divided region; calculating the regional temperature sensitivity and the regional humidity sensitivity of each divided region by using a logistic regression method, analyzing the temperature and humidity balance condition of the planting region, determining the temperature or humidity of the planting region, and selecting a temperature and humidity regulation sequence; and the temperature or humidity of the planting area is adjusted by integrating the area temperature sensitivity or the area humidity sensitivity and the temperature and humidity balance condition of each divided area.

The invention relates to a cultivation method of morchella esculenta, and belongs to the technical field of industrialized production of edible mushrooms. The method comprises the steps of culture medium preparation and laying, sowing, exogenous nutrient supplement, mycelium culture, primordium induction, primordium differentiation, young mushroom and maturity management, second flush mushroom management and the like. Mountain immature soil, rice hull powder, chestnut sawdust and the like are selected as main materials of a used culture medium, peracetic acid fermentation treatment is combined, and traditional formaldehyde treatment which is high in cost and harmful to the environment is replaced. Fermentation type nutrition bricks are adopted as exogenous nutrition materials, and on-site preparation and sterilization operation are replaced. Natural light is combined with LED light supplement, temperature and humidity environments are regulated and controlled in a partitioned mode, energy consumption is reduced, and the thallus development stability is improved. Unified management and batch harvesting are achieved through a multi-layer frame and standard box modular structure. The method has the advantages of low production cost, simplicity in technical implementation, stable yield, suitability for large-scale popularization and the like.

The invention provides application of phosphatidyl ethanolamine in improving resistance of morchella to white mold. Experimental verification shows that exogenous addition of the phosphatidyl ethanolamine can enhance the resistance of the morchella to pseudomonas longispora, so that the resistance of the morchella to the white mold is improved, the development of the white mold is inhibited, the growth of a morchella sporocarp is promoted, and the application possibly has huge potential in the aspect of development of morchella white mold prevention and treatment products.

This invention discloses a method and system for visual recognition and state characterization of cap pores during the drying process of morel mushrooms. The method first constructs a region of interest (ROI) on the morel cap, then performs instance-level recognition and segmentation of individual pores on the cap surface to obtain accurate pore segmentation results. Next, based on the segmentation results, it extracts pore structural features and cap morphological and color features from the ROI. Subsequently, it fuses these three types of features to construct a multi-dimensional feature vector, establishing a unified analytical framework for local pores and the overall cap state. Finally, it inputs the multi-dimensional feature vector into a pre-trained regression model to output the morel mushroom drying state characterization results. This method overcomes the limitations of traditional techniques in treating pores holistically, effectively avoiding problems such as pore adhesion and missed detection, improving the robustness of feature characterization and the consistency of quantitative results, achieving accurate monitoring of the morel mushroom drying process, and providing reliable technical support for drying state characterization and quality control.

The utility model belongs to the technical field of fungus cultivation devices, and particularly relates to an under-forest morchella planting soil detection device which comprises a base and a collection and detection device. The collecting and detecting device is provided with a conveying channel, a plurality of springs are fixedly installed at the bottom of the conveying channel, a fixing frame is fixedly installed at the bottoms of the springs, and a first servo motor is fixedly installed on one side of the fixing frame; the movable arm moves circumferentially with the joint of the fixing frame as the axis, the position of a second servo motor at one end of the movable arm is adjusted, and when the movable arm moves to the designated position, the second servo motor is driven to enable a second ball screw at the output end to rotate clockwise to be in threaded fit with a second ball nut; and a second ball nut is matched with the grab bucket in a hinged mode, so that the grab bucket is turned over with the hinged position of the mounting plate as the axis to collect soil on the ground, the collecting and conveying functions are achieved, the problem that certain labor intensity of workers is consumed during collecting is solved, and the adaptability in use is improved.

The utility model discloses a three-dimensional box convenient to culture morchella, relates to the technical field of morchella culture, solves the problem that the humidifying effect on morchella is not ideal, and comprises a bottom box, the top of the bottom box is fixedly connected with a culture box, and the rear position of the bottom of an inner cavity of the bottom box is rotationally connected with a rotating shaft through a bearing. According to the morchella vulgaris cultivation device, morchella vulgaris stored in the cultivation box can be sprayed with water and humidified by arranging the spray head, the electric air cylinder, the tooth plate and the first gear jointly form a driving mechanism, the rotating shaft can be driven to rotate horizontally, then the spray head can be driven to rotate horizontally, and the horizontal angle of the spray head can be adjusted; and a servo motor, a second gear and a third gear are arranged to jointly construct an adjusting mechanism, a spray head can be driven to turn over, the vertical angle of the spray head can be adjusted, and the spray head can spray water to the morchella in the front-back position.

The utility model relates to the technical field of strain inoculation, and discloses an inoculator for morchella strain transfer, which comprises a bottom plate, an inoculation component is arranged on the left side of the top of the bottom plate, the inoculation component comprises a strain box, and a first motor is fixedly connected to the top of an inner cavity of the strain box. The inoculation assembly is arranged, mycelium pure culture secondary strains reproduced on the basis of mother strains in a strain box can be scattered firstly, then the transfer assembly pushes culture bags containing natural solid culture media upwards through two electric push rods to move the culture bags placed in placing holes, and the culture bags are placed in the placing holes. At the moment, the discharge pipe is inserted into the culture bag, and under the driving of the spiral discharge piece, the materials are uniformly discharged into the culture bag for three-stage culture, so that the transfer work of the strains can be completed, meanwhile, the working intensity of an operator can be reduced, and the pollution caused by frequently taking tools and the influence on the survival rate of the strains are avoided.

The utility model relates to the technical field of morchella esculenta cultivation, and provides an artificial cultivation greenhouse for planting morchella esculenta, which comprises a greenhouse body and a door body frame, the door body frame is fixed on the greenhouse body, an outer frame is arranged outside the door body frame, a connecting assembly is arranged between the outer frame and the door body frame, and a switch assembly is arranged outside the outer frame. The door curtain is connected to the lower end of the top of the door frame, stand columns are fixedly connected to the two sides of the door frame, long cavities are formed in the stand columns, and sliding ways are arranged on the inner side surfaces of the long cavities. According to the technical scheme, the problems that in the prior art, the sealing mode of an existing greenhouse is single, the inlet and the outlet are mainly sealed through a plastic film, the plastic film is prone to being damaged due to abrasion after being repeatedly opened and closed, and the internal environment of the greenhouse is affected are solved.

The utility model relates to the technical field of cultivation and planting frames, in particular to a morchella shelf cultivation and planting frame which comprises a placing frame, the side face of the placing frame is connected with a plurality of placing plates from top to bottom, a plurality of air holes are formed in the tops of the placing plates, and rotating grooves are formed in the tops of the placing plates on the outer sides of the air holes. The inner wall of the rotating groove is rotationally connected with an arc-shaped water sprinkling plate, a water inlet hole is formed in the top of the water sprinkling plate, a plurality of water outlet holes are formed in the side face of the water sprinkling plate, the water inlet hole is connected with one end of a water inlet pipe, the other end of the water inlet pipe is rotationally connected with an upper water inlet pipe, and the top end of the upper water inlet pipe is connected with the bottom of a transverse water pipe. According to the device, the side faces of long cylindrical bagged nutrients are evenly sprayed, air can be fed through the air holes in the using process, and the situation that the bottom faces of the bagged nutrients make contact with the containing face and cannot make contact with the air, consequently, morchella hypha development is uneven, and morchella cultivation is affected is prevented.

The utility model relates to the technical field of morchella esculenta production, and discloses a morchella esculenta culture shelf which comprises two supporting plates which are distributed up and down at an interval and a side shelf with the two ends fixedly connected with the supporting plates, culture assemblies for placing morchella esculenta stock seeds are arranged between the supporting plates, and the two culture assemblies are arranged in a front-and-back symmetry mode. The two culture assemblies are supported and fixed through the supporting assemblies and the supporting plates. The stock culture shelf has the advantages that a one-groove culture mode is adopted, the placing space of the culture shelf is reasonably utilized, stock seeds cannot be stacked when placed and cultured, taking out or placing operation of each stock seed becomes simple and fast, meanwhile, the air circulation on the shelf is guaranteed, and meanwhile the culture shelf is convenient to use. And the hypha development condition of the stock seeds can also be visually observed, so that the stock seeds are convenient to culture, load and take, and the orderliness and efficiency of production are improved.

The invention discloses a toadstool exogenous nutrition bag with corn as a main material and a preparation method and application of the toadstool exogenous nutrition bag, and belongs to the technical field of edible fungus cultivation. The toadstool exogenous nutrition bag provided by the invention comprises wheat, corn in a semi-crushed state and rice husks in a mass ratio of 1: 2. Experimental results show that when the exogenous nutrition bag is used for producing morchella esculenta, the time for hyphae to overgrow the exogenous nutrition bag can be shortened, infectious microbe breeding is reduced, and morchella esculenta can be fruiting in advance; the yield of the morchella esculenta can be greatly increased, and the highest average yield can reach 270.8 kg / 667 m < 2 > under the cold greenhouse condition and is greatly increased compared with the common yield of 100 kg / 667 m < 2 > in the local area. A new exogenous nutrition bag is provided for low-cost and high-efficiency production of morchella esculenta, wheat is replaced with corn, the replacement rate is 66.67%, the food safety pressure is relieved, and the method has wider applicability; the yield of the morchella esculenta can be remarkably increased, and the method has outstanding popularization value.

The invention discloses a flavor enhancer with a compound flavor of morchella esculenta and shiitake mushrooms as well as a preparation method and a production line of the flavor enhancer. The flavor enhancer comprises the following raw material components in parts by mass: 10-50 parts of toadstool flavor powder, 5-40 parts of a toadstool Maillard reaction product, 0.08-8 parts of toadstool flavor peptide, 10-50 parts of shiitake mushroom flavor powder, 5-40 parts of a shiitake mushroom Maillard reaction product and 0.08-8 parts of shiitake mushroom flavor peptide. Morchella and shiitake mushrooms are cultured through liquid fermentation, toadstool umami substances are extracted through combination of enzymolysis and ultrasonic waves, toadstool umami peptides are prepared through a multi-step ultrafiltration method, and a Maillard reaction is conducted on toadstool fermentation liquor and shiitake mushroom fermentation liquor which are subjected to liquid fermentation culture; the shiitake mushroom umami substances are extracted through supercritical CO2, and shiitake mushroom umami peptides are prepared through a multi-step ultrafiltration method; all the components are mixed according to a proper proportion to form the flavor enhancer with the composite flavor. The problems that in the prior art, the extraction efficiency is low, active ingredients are prone to inactivation, and the flavor stability is poor are solved.

The utility model discloses a morchella planting device relates to morchella planting technical field, including roof and its multiple spray pipes, the roof bottom is installed with adjusting mechanism, the utility model has the advantages of (beneficial effect) the rotation of rotating rod drives drive rod and transmission rod to form continuous transmission chain, and then realizes the unified drive control of spray pipe distribution mechanism, and transmission rod realizes the synchronous force transmission of multiple points through the connection with a plurality of linkage rods, drives the smooth linear motion of multiple sliding blocks in slide rail, and each sliding block is translated with the same rate and displacement, guarantees the overall structure synchronism and the uniformity of spraying, and each sliding block is connected with a connecting rod and drives the synchronous movement of spray pipe, and multiple spray pipes are expanded with equal interval under linkage structure, can be flexibly adjusted according to different planting area, realizes the regular arrangement and global coverage of spray pipe, effectively promotes water resource utilization efficiency and the consistency of mushroom cultivation environment.

This invention relates to a method for rapidly screening filamentous fungi that produce high levels of triglycerides and polyunsaturated fatty acids, belonging to the field of microbial technology. The method employs a two-stage screening strategy of "resistance primary screening – fluorescence secondary screening." In the primary screening stage, strains with strong lipid synthesis capabilities are initially screened by adding a combination of phytocyanin and quizalofop-p-ethyl inhibitors to the culture medium. In the secondary screening stage, cellophane culture technology and Nile red fluorescence staining are combined to quantitatively assess the triglyceride content of the colonies, achieving rapid and non-destructive screening. This method is simple to operate, highly efficient, and shows a significant positive correlation between fluorescence intensity and triglyceride content. It is suitable for mutagenesis breeding and industrial strain selection of oil-producing filamentous fungi such as *Morchella alpineensis*.

The invention relates to the field of morchella esculenta cultivation, and discloses a morchella esculenta cultivation device and cultivation method.The morchella esculenta cultivation device comprises a pair of first supporting frames; the multiple cultivation frames are arranged between the pair of first supporting frames in the circumferential direction. The driving mechanism is arranged on any one of the first supporting frames, the driving mechanism is connected with the multiple cultivation frames, and the driving mechanism is used for driving the multiple cultivation frames to rotate in the circumferential direction; the driven mechanism is arranged on the other first supporting frame, the driven mechanism can be connected with the multiple cultivation frames, and the driven mechanism can enable the multiple cultivation frames to be kept in a horizontal state when the multiple cultivation frames rotate in the circumferential direction; the second support frame is connected with the first support frame close to the driving mechanism; the irrigation mechanism is slidably arranged on the second supporting frame in the height direction, and the irrigation mechanism is located on one side of the multiple cultivation frames, by means of the morchella esculenta cultivation device, morchella esculenta on each layer of cultivation frame can obtain sufficient illumination and sufficient water, and the quality of morchella esculenta is improved.