99 results about "Morchella" patented technology

This invention discloses a method and system for visual recognition and state characterization of cap pores during the drying process of morel mushrooms. The method first constructs a region of interest (ROI) on the morel cap, then performs instance-level recognition and segmentation of individual pores on the cap surface to obtain accurate pore segmentation results. Next, based on the segmentation results, it extracts pore structural features and cap morphological and color features from the ROI. Subsequently, it fuses these three types of features to construct a multi-dimensional feature vector, establishing a unified analytical framework for local pores and the overall cap state. Finally, it inputs the multi-dimensional feature vector into a pre-trained regression model to output the morel mushroom drying state characterization results. This method overcomes the limitations of traditional techniques in treating pores holistically, effectively avoiding problems such as pore adhesion and missed detection, improving the robustness of feature characterization and the consistency of quantitative results, achieving accurate monitoring of the morel mushroom drying process, and providing reliable technical support for drying state characterization and quality control.

The utility model relates to the technical field of morchella esculenta production, and discloses a morchella esculenta culture shelf which comprises two supporting plates which are distributed up and down at an interval and a side shelf with the two ends fixedly connected with the supporting plates, culture assemblies for placing morchella esculenta stock seeds are arranged between the supporting plates, and the two culture assemblies are arranged in a front-and-back symmetry mode. The two culture assemblies are supported and fixed through the supporting assemblies and the supporting plates. The stock culture shelf has the advantages that a one-groove culture mode is adopted, the placing space of the culture shelf is reasonably utilized, stock seeds cannot be stacked when placed and cultured, taking out or placing operation of each stock seed becomes simple and fast, meanwhile, the air circulation on the shelf is guaranteed, and meanwhile the culture shelf is convenient to use. And the hypha development condition of the stock seeds can also be visually observed, so that the stock seeds are convenient to culture, load and take, and the orderliness and efficiency of production are improved.

The utility model discloses a morchella planting device relates to morchella planting technical field, including roof and its multiple spray pipes, the roof bottom is installed with adjusting mechanism, the utility model has the advantages of (beneficial effect) the rotation of rotating rod drives drive rod and transmission rod to form continuous transmission chain, and then realizes the unified drive control of spray pipe distribution mechanism, and transmission rod realizes the synchronous force transmission of multiple points through the connection with a plurality of linkage rods, drives the smooth linear motion of multiple sliding blocks in slide rail, and each sliding block is translated with the same rate and displacement, guarantees the overall structure synchronism and the uniformity of spraying, and each sliding block is connected with a connecting rod and drives the synchronous movement of spray pipe, and multiple spray pipes are expanded with equal interval under linkage structure, can be flexibly adjusted according to different planting area, realizes the regular arrangement and global coverage of spray pipe, effectively promotes water resource utilization efficiency and the consistency of mushroom cultivation environment.

This invention relates to a method for rapidly screening filamentous fungi that produce high levels of triglycerides and polyunsaturated fatty acids, belonging to the field of microbial technology. The method employs a two-stage screening strategy of "resistance primary screening – fluorescence secondary screening." In the primary screening stage, strains with strong lipid synthesis capabilities are initially screened by adding a combination of phytocyanin and quizalofop-p-ethyl inhibitors to the culture medium. In the secondary screening stage, cellophane culture technology and Nile red fluorescence staining are combined to quantitatively assess the triglyceride content of the colonies, achieving rapid and non-destructive screening. This method is simple to operate, highly efficient, and shows a significant positive correlation between fluorescence intensity and triglyceride content. It is suitable for mutagenesis breeding and industrial strain selection of oil-producing filamentous fungi such as *Morchella alpineensis*.

The invention provides bacillus altitudinis CL6 with a morchella esculenta continuous cropping resisting effect and application of the bacillus altitudinis CL6, and belongs to the technical field of microorganisms. The bacillus altitudinis CL6 with a continuous cropping resisting effect on morchella is separated from morchella rhizosphere soil and is preserved in the China Center for Type Culture Collection on March 18, 2025, and the preservation number is CCTCC NO: M 2025516. The bacillus altitudinis CL6 disclosed by the invention can be used as an auxiliary added beneficial microbial agent in the cultivation production of the morchella to resist the continuous cropping harm of the morchella, and finally, the cultivation stability of the morchella is improved. The strain provided by the invention can promote the continuous cropping resistance effect of morchella esculenta and increase the yield of morchella esculenta. And a green and efficient anti-continuous cropping biological solution is provided for the morchella esculenta cultivation industry.

The invention discloses a method for efficiently and greenly cultivating morchella esculenta in a cherry orchard, and belongs to the technical field of edible mushroom cultivation. According to the technology, morchella esculenta interplanting cultivation is carried out in an idle period (May to November) from cherry orchard harvesting to leaf falling; the method comprises the key technologies of cherry root system protection type shallow soil treatment, arched shed type precise sun shading, green soil disinfection of quicklime, high-temperature shed braising and biological agents, compounding of waste with exogenous nutrition bags, pest and disease damage prevention and control through biological agents, mushroom dreg returning-to-field ecological circulation and the like. The technical problems that during interplanting of morchella esculenta on fruit trees, root systems are prone to damage, chemical agent residues exist, the facility cost is high, and the land utilization rate is low are solved. By adopting the method, the Mu yield of the morchella can reach 200-250kg, the production cost is saved by more than 25%, the pesticide residue meets the green food standard, the ecological cycle cultivation of the cherries and the morchella is realized, and the land utilization efficiency and the comprehensive economic benefit are remarkably improved.

The water-saving sprinkling irrigation device comprises a positioning barrel and a fixing rod, a sprinkling irrigation mechanism used for sprinkling irrigation is arranged in the positioning barrel, and a reinforcing mechanism used for reinforcing the sprinkling irrigation mechanism is arranged at the top of the positioning barrel. And collecting mechanisms for recovering dripped and leaked water at the connecting position are arranged on the two sides of the positioning barrel and correspond to the water inlet position of the sprinkling irrigation mechanism, and the outer portion of the positioning barrel is in threaded connection with a flow dividing mechanism through a threaded groove. The spray irrigation mechanism is mounted in the positioning barrel, so that adjustment can be performed according to the water replenishing position of the morchella esculenta, deformation of the spray irrigation mechanism during spray irrigation can be prevented by the reinforcing mechanism, and the collecting mechanism is arranged at the joint of the spray irrigation mechanism, so that the spray irrigation mechanism is more convenient to use. In this way, when water dripping occurs, the dripped and leaked water can be collected into the positioning barrel for centralized collection, and then the collected dripped and leaked water can be dripped to the periphery of the positioning barrel through the threaded groove and the flow dividing mechanism.

The invention discloses a toadstool white mould disease biocontrol bacterial preparation and a preparation method thereof, and relates to the technical field of microbial bactericides, the toadstool white mould disease biocontrol bacterial preparation comprises the following raw materials by weight: 37.5 parts of Bacillus firmus fermentation broth, 0.5 part of a nano-material, 45 parts of a carrier, 5 parts of a wetting agent, 10 parts of a dispersant and 2 parts of a protective agent, the nano-material is silica, the carrier is diatomite, the wetting agent is Tween 20, and the dispersant is diatomite. The dispersant is polyvinyl alcohol, and the protective agent is dextrin; according to the toadstool white mould disease biocontrol bacterium preparation and the preparation method thereof, biocontrol bacteria are preferentially colonized at key sites such as plant rhizosphere, leaf surface or wound and the like, so that limited ecological niche is occupied, and effective infection is difficult to establish due to lack of physical space and attachment sites when pathogenic bacteria invade; by adding the nano material, the carrier, the wetting agent, the dispersing agent and the protective agent, the environmental stability and the field persistence of the biocontrol bacterium are improved.

The invention discloses a method for planting carya illinoensis under forest and morchella esculenta, and belongs to the technical field of mushroom planting. The method specifically comprises the steps of forest land selection, deadwood and weed removal, ploughing and soil preparation treatment, greenhouse building, soil improvement, soil disinfection, ditching and ridging, morchella esculenta sowing, watering, temperature and humidity management, nutrition management, management after fruiting and harvesting. According to the method for planting the carya illinoensis under the forest and the morchella esculenta, efficient utilization of the land under the forest is achieved, cost input is reduced, planting benefits are increased, planting of the morchella esculenta under the forest in China is promoted, and a new thought is provided for development of characteristic economy under the forest and production of edible mushrooms in China.

The invention relates to the technical field of planting of cremastra appendiculata, and particularly discloses fertilizing equipment and a planting method of cremastra appendiculata. The planting method of cremastra appendiculata comprises the following steps: S1, cleaning land and applying a mixed disinfectant; s2, ridging and transplanting sagittaria trifolia seedlings; s3, spraying a targeted agent and an amino acid foliar fertilizer regularly; and S4, continuous field management is conducted until digging treatment is conducted in a proper period, fertilization equipment is applied to the sagittaria trifolia planting method for application of amino acid foliar fertilizer, the fertilization equipment structurally comprises a driving vehicle, an n-shaped box, a fertilization assembly and a recycling assembly, and the fertilization assembly cooperates with front and back spraying heads through a shifting rod to achieve all-directional spraying of leaves. According to the invention, the risk of plant diseases and insect pests is reduced through full-period planting management, the growth vigor of plants is improved, a foliar fertilizer is efficiently sprayed to the front and back surfaces of leaves by using the fertilizing equipment, and meanwhile, the redundant fertilizer liquid is recycled, so that the waste and the planting cost are reduced, and the planting efficiency is improved. High quality and high yield of the cremastra appendiculata

The utility model discloses efficient self-control type morchella strain culture equipment, which relates to the technical field of morchella strain culture and comprises a culture box, a humidifier arranged on the culture box and a PLC (programmable logic controller) touch screen all-in-one machine. A plurality of partition plates are sequentially arranged in the incubator from top to bottom at equal intervals, a plurality of storage racks are arranged at the tops of all the partition plates and the bottom of the interior of the incubator in the length direction at equal intervals, each storage rack is of a hollow disc structure, and the storage racks are used for containing bagged nutrients; diversion pipes are arranged at the bottoms of all the partition plates and the inner top of the culture box, and the diversion pipes are communicated with a humidifier through pipelines; a rotatable humidifying pipe is arranged below the flow dividing pipe, and the humidifying pipe is used for rotating to spray water to humidify the bagged nutrients on the storage rack; a temperature and humidity sensor is arranged in the incubator; the morchella hyphae are uniformly developed, the efficient cultivation of the morchella is facilitated, and the practicability is improved.

The invention discloses Mortierella elongata and application of the Mortierella elongata, the strain number of the Mortierella elongata is JSAFC 247, the Mortierella elongata is preserved in the China General Microbiological Culture Collection Center on December 9, 2024, and the preservation number is CGMCC No.41703. The Mortierella elongata has the advantages that the Mortierella elongata can be used for preparing the Mortierella elongata and the application of the Mortierella elongata; the mortierella elongata strain provided by the invention is strong in pathogenicity to larvae of the larvae of the corpulenta aeruginosa, is environment-friendly and pollution-free, is not easy to generate drug resistance, can be used as a living body biopesticide, and has very strong application potential in biological prevention and control of the corpulenta aeruginosa.

The invention discloses a microdomain environment building method for imitating wild cultivation under a morchella forest, and relates to the technical field of edible fungus cultivation, the method comprises the following steps: selecting an under-forest area with canopy density of 0.7-0.9 for ecological transformation, applying an organic material, and disinfecting; the temperature is controlled to be 15-20 DEG C by comprehensively applying canopy shading, a sunshade net, a small arched shed and the like, the air humidity is maintained to be 80%-90% through monitoring of a sensor and spraying or drip irrigation, and the soil water content is controlled to be 60%-70%; a bionic matrix formed by mixing wood sections, branches and fallen leaves is laid, and after strains are sown, the seedlings are covered with nutrient covering soil formed by mixing under-forest humus soil, turfy soil and vermiculite; selecting high-quality strains for inoculation; an Internet of Things system is arranged to monitor temperature, humidity, illumination and COO in real time, and environment factors are automatically adjusted; performing fine management according to the growth stage and adopting green prevention and control; the method can significantly improve the yield, quality and cultivation success rate of morchella esculenta, and has the advantages of being eco-friendly, low in cost and replicable.

The invention discloses an HPV virus inactivation dressing and a preparation method thereof, and relates to the field of HPV virus inactivation dressings, the HPV virus inactivation dressing comprises, by weight, 50-90 parts of animal extract, 5-15 parts of morchella, 5-10 parts of sodium carboxymethyl cellulose, 5-10 parts of gelatin and 30-35 parts of purified water. Through the synergistic effect of the animal extract, the morchella esculenta and special substances, HPV viruses can be efficiently inactivated, and the detergent has sterilization and anti-inflammation effects and is suitable for various detergent products; through the synergistic effect of the animal extract and the morchella esculenta, the HPV virus can be efficiently inactivated, and experimental results show that the inactivation rates of HPV16 type pseudoviruses and HPV18 type pseudoviruses reach 90.53% and 98.79% respectively.

The invention discloses early-maturing and high-yield morchella septimelata and a molecular marker thereof. The preservation number of the morchella septimelata is CCTCC (China Center For Type Culture Collection) NO: M 20252848. The whole anti-season planting period of the strain provided by the invention is 50-60 days, and is shortened by about 20 days compared with that of a conventional septimelate variety; the whole period of in-season cultivation (sowing in 11-December) is 60-90 days and is shortened by about one month compared with that of a conventional sextelate variety, fruiting can be achieved before the Spring Festival, and the high-temperature risk in spring is effectively avoided; the yield is improved by more than 50% compared with that of a conventional variety, and white mold infection is not easily caused. The invention also develops a strain specific InDel marker and a primer, which can be used for variety authenticity identification.

The invention relates to a method for indirectly cultivating a morchella strain bag, and belongs to the technical field of edible mushrooms. The invention provides a method for indirectly cultivating a morchella strain bag. The method comprises the following steps: (1) indirectly cultivating a strain bag; 2) covering with a mulching film; (3) bag cultivation and fruiting management, wherein bag indirect cultivation comprises outdoor cultivation and indoor frame cultivation. According to the method, a traditional toadstool cultivation method based on a nutrition bag is changed, the method is different from a secondary nutrition bag-free cultivation method, the bag opening is sealed by using the vigorous toadstool strain, the situation that the nutrition bag is polluted by infectious microbes in soil after the bag is buckled is avoided, meanwhile, bidirectional growth of toadstool hyphae in the soil and the nutrition bag matrix can be achieved, and the yield of the toadstool is increased. Nutrient components of the nutrition bag can be conveyed to hyphae growing in soil, the purposes of efficiently utilizing nutrition and improving the yield are achieved, sowing and nutrition bag placing are completed at a time, and raw materials and labor cost for nutrition bag manufacturing, sowing, arch building, film covering and the like are saved by 20%-30%.

The utility model discloses a morchella esculenta soil ditching and seeding device with a soil covering function, and relates to the technical field of ditching and seeding devices. Comprising a supporting frame, a connecting structure is arranged on the upper surface of the supporting frame, rolling wheel assemblies are vertically arranged at the left end and the right end of the front side of the supporting frame in a sliding mode respectively, a fixed connecting piece is arranged in the middle of the rear side of the supporting frame, and a first ploughing assembly is vertically arranged at the bottom of the fixed connecting piece; the left end and the right end of the rear side of the supporting frame are each provided with a fine adjustment connecting piece, and second ploughing assemblies are arranged at the bottoms of the fine adjustment connecting pieces in a sliding mode. And through the design of the fine adjustment connecting piece, the whole morchella soil ditching and seeding device is more flexible.

The invention discloses a through-type morchella picking device, and particularly relates to the technical field of morchella picking, the through-type morchella picking device comprises a groove plate, a cavity cutter, a plurality of spraying holes, a sleeving block and a picking driving part, and the cavity cutter used for cutting and picking morchella is slidably arranged on the inner wall of the groove plate; the multiple spraying holes are formed in one side of the inner wall of the cavity cutter, multiple dispersing holes are formed in one side of each spraying hole, and a cavity used for being filled with fresh-keeping liquid is formed in the cavity cutter; the sleeving block is fixed on one side of the cavity cutter; and the picking driving piece is mounted in the sleeving block. The device has the advantages that fresh-keeping integrated treatment is carried out on the bottom truncation surface and the edge part of the truncation surface at the moment of cutting and picking the morchella, the integrated synchronous treatment of picking and fresh-keeping is realized, the problem of omission caused by traditional subsequent spraying is effectively avoided, and the processing efficiency of picking and fresh-keeping of the morchella is greatly improved; therefore, the problem that the processing efficiency of morchella picking and preservation is greatly reduced is solved.

The utility model discloses an auxiliary tool for picking morchella. The auxiliary tool comprises a box body and feeding ports which are linearly arrayed on the side wall of the box body, the reeds are linearly arrayed in the box body and are fixedly connected with the feeding port; the storage racks are arranged in the box body in a linear array mode and located at the bottoms of the reeds, and bacterium storage grooves in a linear array mode are formed in the top ends of the storage racks. According to the morchella picking auxiliary tool, morchella can fall onto the storage frame in the sliding process, morchella colonies can automatically slide into any one of the morchella storage grooves to be stored when on the storage frame, so that the morchella can be collected, the morchella entering the morchella storage grooves can only be arranged in sequence subsequently, and the morchella picking efficiency is improved. The toadstool collecting device has the advantages that the toadstools are prevented from being stacked together, the toadstools are prevented from being subjected to large pressure during stacking, the picked toadstools can be protected, the toadstool collecting effect is improved, and waste caused by damage to the toadstools is reduced.

The present application relates to the technical field of morchella cultivation, and discloses a morchella cultivation method and a cultivation device, which are characterized by the following: the cultivation bed is arranged underground, the underground humid and dark environment can be fully utilized, the cultivation of the mycelium is facilitated, the underground environment is easier to keep warm and humid, the difficulty and cost of artificial regulation of temperature and humidity are greatly reduced, in addition, the underground cultivation bed fully utilizes the height difference between the underground and the ground, the longitudinal height requirement of the arched mushroom shed is greatly reduced, the manufacturing cost of the mushroom shed is effectively saved, the arched mushroom shed is not easily attacked by wind and snow in bad weather due to its low height, the ability of the arched mushroom shed to resist natural disasters such as violent wind and snow is effectively enhanced, the service life of the arched mushroom shed is effectively improved, the accidental risk in the cultivation process is greatly reduced, the yield of morchella cultivation is effectively ensured, and the arched mushroom shed has the advantages of good temperature regulation uniformity and high temperature regulation efficiency.

This invention discloses a cultivation method using undisturbed soil from wild morel mushroom cultivation sites, belonging to the field of morel mushroom cultivation technology. The method involves excavating undisturbed soil from wild morel mushroom growing areas, preparing mushroom beds in cultivation areas, transferring the undisturbed soil to the prepared mushroom beds, leveling it, spraying with a composite film-forming liquid, and compacting it. Nutrient bags containing morel mushroom substrate are placed on the prepared undisturbed soil. During cultivation, an arched shed is constructed, controlling the temperature at 16-18℃ and the humidity at 60-75%, and cultivating morels under conditions of 500-700 lumens of light flux. Ventilation is performed every two days. Morels can be harvested when the cap diameter reaches 5-7 cm. This method preserves the microbial community and nutrients in the undisturbed soil, making it suitable for the growth and reproduction of wild morels, thereby improving the cultivation efficiency of wild morels and protecting wild morel resources.

The application discloses an indoor seedling raising method of Tricholoma mongolicum, and relates to the technical field of indoor seedling raising, which comprises the following steps: preparing a low-nutrient symbiotic germination culture medium, taking water and agar as the basis, containing 0-5 g / L sugar substances and humus soil extract, and forming a sterile environment beneficial to the symbiosis of fungi; selecting associated fungi to prepare mycelium fragment suspension as symbiotic inoculants through liquid fermentation; performing surface disinfection and sterile ultrasonic treatment on Tricholoma mongolicum seeds; sowing the seeds on the surface of the culture medium and inoculating the symbiotic inoculants under sterile conditions, and culturing under artificial climate control of temperature and light, so as to promote the symbiotic relationship between the fungi and the seeds; and obtaining symbiotic seedlings after the seeds swell and differentiate into protocorms or young leaves. The symbiotic germination mechanism of associated fungi is introduced into the indoor sterile culture system, the emergence rate can reach more than 90%, the emergence uniformity is high, the seedlings are not limited by seasons and climate, the seedlings are self-brought mycorrhizal roots, the transplanting survival rate is high, and the reliable seedling guarantee is provided for the large-scale artificial planting of Tricholoma mongolicum.

The utility model provides a quick morchella seeding device, which relates to the field of morchella seeding and comprises a support, a limit frame, a plurality of seeders, an extension frame, a rotating shaft, a moving wheel, a plurality of seeders, a funnel, a limit component, a ditching block, a driving wheel and a handle. The rotating shaft drives the internal material stirring disc to rotate in the rotating process, the material stirring disc is located in the seeder and rotates along with the rotating shaft, the material stirring disc is triangular, the three corners of the material stirring disc are attached to the inner wall of the seeder, seeds in the seeder can be stirred through driving of the rotating shaft, and regular seeding of morchella is facilitated; the bracket is supported by the moving wheels and the driving wheels, so that the bracket keeps relatively stable movement, the bracket drives the ditching block to ditch the land when moving, and the stable movement of the bracket enables the ditching depth of the ditching block to be relatively uniform, so that the subsequent sowing depth of morchella is relatively uniform, and the survival rate of morchella is increased.

The utility model relates to the technical field of morchella cultivation frame, and disclose a culture frame for morchella cultivation, including mounting bracket, be provided with placing device on mounting bracket, and placing device includes installation department. The culture frame for morchella cultivation, through setting fixed block, first stainless steel wire rope, rope roller, pivot, protective housing, worm wheel, worm, fixed frame and second stainless steel wire rope, rotate the worm, drive worm wheel and rope roller rotation, use first stainless steel wire rope and second stainless steel wire rope and place the plate hoist, expand the interval between the placement board, facilitate the air flow and the growth of morchella, reverse rotation worm, make the placement board move down, facilitate to the placement board on setting morchella culture box and take; through setting first sleeve and second sleeve, can both to the protection of second stainless steel wire rope, also can support the placement board when the placement board moves down, prevent the placement board to move down, press the morchella and break down.

The utility model discloses an irrigation device for morchella esculenta planting, which relates to the field of morchella esculenta planting and comprises a fixing rod, and two ends of the fixing rod are respectively connected with a support of a greenhouse in a welding manner; the driving part is arranged above the fixed rod and is used for driving the device to move in the greenhouse; the stabilizing piece is installed on the driving piece, and the stabilizing piece is matched with the driving piece to be used for keeping the device stable on the fixing rod; the spraying part is installed at the bottom of the driving part, the spraying part is arranged on the lower gear of the fixing rod, and the spraying part is used for spraying the nutrient solution to morchella in the greenhouse. The stability of movement on the fixing rod is enhanced through the stabilizing piece. A connecting rod in the stabilizing piece is movably connected with an extension plate through a rotating shaft, a guide wheel is attached to the lower surface of a fixing rod by means of resetting of a torsional spring, the guide wheel and the torsional spring are matched to play a role in stabilizing and guiding, shaking of the device during moving is reduced, it is ensured that the irrigation process is more stable, and the irrigation effect is improved.

The utility model discloses an automatic morchella inoculation device which comprises a base, two supporting blocks are fixedly connected to the top of the base, a tank body is fixedly connected to the tops of the supporting blocks, a fixing plate is arranged in the tank body, a second limiting groove is formed in the fixing plate, and the second limiting groove is fixedly connected to the top of the base. A first limiting groove is formed in the fixing plate, and a clamping block is slidably connected to the outer portion of a second limiting groove. By means of the structure, the sliding column is rotated to enable the clamping block outside the sliding column to move in the second limiting groove and the third limiting groove in the lantern ring, when the clamping block moves into the first limiting groove, the fixing plate in the tank body can be dismantled, and therefore a worker can check all parts of the tank body more conveniently and rapidly; according to the inoculation device, the problems of abrasion, damage or blockage and the like can be found in time, maintenance or replacement is carried out, normal operation of the inoculation device is ensured, and the structure in the tank body can be conveniently modified or part of components can be conveniently replaced so as to meet new process requirements.

This invention provides a genetically engineered *Yersinia lipolytica* strain and its applications. The genetically engineered strain is a *Yersinia lipolytica* Po1f strain with the PEX10 gene knocked out, expressing the C16 / 18 elongase gene MaELO3 from *Morchella alpina*, the elongase gene AtKCS from *Arabidopsis thaliana*, and the elongase gene CraKCS from *Brassica oleracea*. The strain also expresses the elongase gene CgKCS from *Capsella*. Further expression of the desaturase gene MaD15D from *Morchella alpina*, and overexpression of the strain's own diacylglycerol transferase gene DGA1 and its own Δ9 desaturase gene OLE1, resulted in a nervonic acid yield of 111.6 mg / L in shake-flask fermentation. By adding 1% rapeseed oil exogenously, the nervonic acid yield reached 185.1 mg / L. The *Yersinia lipolytica* genetically engineered strain constructed in this invention is simple to operate, has stable and reliable performance, and can be applied to large-scale commercial production.

The invention belongs to the technical field of agricultural planting greenhouses, and particularly relates to a double-layer heat preservation arched shed for morchella planting based on orchard intercropping. The fixed shed is fixedly arranged on the ground; the driving mechanism is fixedly arranged on the ground, and one end of the driving mechanism is located in the fixed shed; the movable shed is in threaded connection with the driving mechanism, and the bottom end of the movable shed is in sliding sealing contact with the ground; the unfolding and storing mechanism is arranged on the driving mechanism, one end of the unfolding and storing mechanism is fixedly connected with the fixed shed, and the other end of the unfolding and storing mechanism is fixedly connected with the movable shed; when the driving mechanism drives the movable shed to move in a reciprocating mode, the movable shed drives the unfolding and storing mechanism to be unfolded and / or stored. By means of the multi-layer covering design, it is ensured that the temperature in the greenhouse is kept stable in the low-temperature season; meanwhile, an adjustable sun-shading system is arranged so as to adapt to the demand changes of morchella in different growth periods, and can efficiently cooperate with the orchard environment to achieve accurate regulation and control of light, temperature, water and fertilizer resources.

The utility model discloses a cleaning device for dried morchella esculenta, and relates to the technical field of morchella esculenta processing. The device comprises a cleaning pool, electric push rods are arranged at the two ends of the cleaning pool, the piston ends of the two electric push rods are connected with a mounting frame, a guide rod is rotationally arranged on the mounting frame, one end of the guide rod is connected with a motor arranged on the mounting frame, and two circular plates are fixedly arranged on the guide rod; a circular plate and a net cylinder are arranged on a guide rod, so that a storage net bag is formed, a partition plate frame is arranged on the guide rod to divide the storage net bag into a plurality of areas, bristle rods are rotationally arranged in the areas, morchella is separately placed in the areas, the storage net bag sinks into clear water, the morchella can be effectively soaked in the clear water, and the morchella can be effectively soaked in the clear water. When the storage net bag rotates in water, not only can the morchella be driven to move, but also water flow can be stirred to be matched with rotary scraping and brushing of the bristle rod, so that the morchella is effectively cleaned, and the practicability is improved.

The utility model discloses a morchella culture device which comprises supports, two universal wheels are sequentially and fixedly connected to the bottom ends of the supports from front to back, two culture boxes are sequentially arranged between the two supports from top to bottom, cavity blocks are fixedly connected to the two ends of each culture box, and the cavity blocks are fixedly connected with the contact faces of the supports. Through cooperation of a support, universal wheels, a cavity block, a cultivation box, soil, morchella esculenta and a water supplementing unit, a proper amount of clear water is injected into a transverse pipe in a water passing pipe through a three-way pipe, and at the moment, due to the fact that a first water outlet hole and the clear water in the transverse pipe are in a non-communication state, the situation that water flows out first towards a water inlet is avoided; after the transverse pipe is filled with water, the transverse pipe is moved towards one side at the moment, the second water outlet holes can be aligned with the first water outlet holes, due to the fact that the water pressure in the transverse pipe is uniform, the multiple second water outlet holes can uniformly supplement water into the soil, and batch high-quality culture of the morchella esculenta is facilitated.