The application relates to the technical field of traditional Chinese medicine, and particularly provides a characteristic spectrum of a Shuangge anti-diarrhea preparation and a construction method thereof. The application adopts a multi-wavelength switching technology by screening a sample processing method and optimizing chromatographic conditions, and simultaneously characterizes main efficacy components of six medicines in a prescription. The method can evaluate the overall curative effect of the Shuangge anti-diarrhea preparation from a qualitative point of view, and significantly improves the quality control level of the Shuangge anti-diarrhea preparation.