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Screening for strain of steepletop hickory chick and process for preparing strain thereof

A kind of peak Morchella, bacterial strain technology, applied in the field of microorganisms, can solve problems such as morel resources protection, expansion and application are not widely used

Inactive Publication Date: 2006-06-28
云南云菌科技(集团)有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The deficiencies in the prior art are that the hickory chick bacterial classification can not carry out bacterial classification identification with the method for fruiting, can only preliminarily determine whether it is the pure culture of hickory chick according to mycelia morphology and isolate; The bacterial classification of production generally uses solid bacteria
At present, the use of liquid strains for the protection and propagation of morel resources in China is not widely used

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. Inoculate the sporocarp tissue of Morchella apexus on the slant of the culture medium for separation and purification of the above strains, and cultivate them at 18°C ​​for 10 days. Observe and record the separated cultured test tubes every day, remove the polluted test tubes in time, and select the non-polluted ones. , and strains with good growth potential, to obtain morel isolates in test tubes.

[0034] 2. Inoculate the mycelium block of the isolated test tube on the plate culture dish of the above-mentioned agar medium to purify the strain. After 2 days, take the tip mycelium block and inoculate it on the slant of the medium, and cultivate it at 18°C ​​for 7 days to obtain the morel Bacteria purification test tube species.

[0035] 3. Using the test fruiting body and the corresponding hyphae isolates, extract the total DNA according to the SDS method respectively, carry out PCR amplification and ITS sequence determination, compare the ITS sequence of the sample ...

Embodiment 2

[0040] The steps of strain preparation are the same as in Example 1.

[0041] 1. Inoculate the sporocarp tissue of Morchella apexus on the slant of the culture medium for separation and purification of the above-mentioned strains, and cultivate them at 22°C for 6 days. Observe and record the separated cultured test tubes every day, remove the polluted test tubes in time, and select the non-polluted ones. , and strains with good growth potential, to obtain morel isolates in test tubes.

[0042] 2. Inoculate the mycelium block of the isolated test tube on the plate culture dish of the above-mentioned agar medium to purify the strain. After 2 days, take the tip mycelium block and inoculate it on the slant of the medium, and cultivate it at 22°C for 6 days to obtain morel Bacteria purification test tube species.

[0043] 3. Using the test fruiting body and the corresponding hyphae isolates, extract the total DNA according to the SDS method respectively, carry out PCR amplificatio...

Embodiment 3

[0048] 1. Inoculate the fruiting body tissue pieces of Morchella apexus on the slant surface of the above-mentioned bacteria separation and purification medium, and cultivate them at 24°C for 5 days. Observe and record the separated and cultured test tubes every day, remove the polluted test tubes in time, and select the non-polluted ones. , and strains with good growth potential, to obtain morel isolates in test tubes.

[0049] 2. Inoculate the mycelium block of the isolated test tube on the plate culture dish of the above-mentioned agar medium to purify the strain. After 2 days, take the tip mycelium block and inoculate it on the slant of the medium, and cultivate it at 24°C for 5 days to obtain the morel Bacteria purification test tube species.

[0050] 3. Using the test fruiting body and the corresponding hyphae isolates, extract the total DNA according to the SDS method respectively, carry out PCR amplification and ITS sequence determination, compare the ITS sequence of t...

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Abstract

The invention relates to a spire morchella strain screening and bacteria species manufacturing method. It belongs to microorganism technique. The strain morchella conica M0503 is preserved at Chinese microscobial preservation management committee common mircens on August 11th, 2005 year. Its preservation number is CGMCC NO.1437. The formed liquid and solid bacteria species can be used to protect morchella wildlife resource, and increase its nature yield and quality. Thus it has great social, economic, and ecological benefits and broad application prospect.

Description

Technical field: [0001] The invention belongs to the technical field of microbes, and in particular relates to strain screening and strain preparation methods of hickory chick. Background technique: [0002] Morel Morchella is a world-renowned rare and precious wild edible fungus, and Morchella conica is one of the main species of Morchella produced in Yunnan. The artificial cultivation of hickory chick has occasionally been successfully reported, but the repeatability of the cultivation test is poor, so far it cannot be cultivated commercially, and the hickory chick on the market all comes from wild. In recent years, due to excessive collection, unscientific collection methods and no corresponding technical measures, the natural output of wild morels has dropped significantly. Studies have shown that under the natural environment conditions of the production area, the use of artificial strains and supporting technical measures is one of the most economical and effective wa...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01G1/04
Inventor 桂明英刘蓓朱萍郭永红
Owner 云南云菌科技(集团)有限公司
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