The invention discloses an organic fertilizer for enhancing disease resistance of grape plants. The organic fertilizer is prepared from, by weight, 63-68 parts of chicken manure, 55-60 parts of pig manure, 8-12 parts of platycodin, 15-19 parts of pig bone meal, 24-28 parts of feed residue, 20-25 parts of bean cakes, 5-9 parts of humic acid, 4-8 parts of plant ash, 4-7 parts of bryozoatum, 2-5 parts of Kanuma mud, 2-5 parts of ammonium sulfate, 0.5-1.0 part of borax, 1-3 parts of potassium sulphate, 2-5 parts of monopotassium phosphate, 2-5 parts of ammonium molybdate, 0.4-0.8 part of sodium selenite, 8-11 parts of pyrethrum, 13-15 parts of folium artemisiae argyi and 6-10 parts of artemisia capillaries. The invention further discloses a preparation method of the organic fertilizer for enhancing disease resistance of grape plants. The organic fertilizer can enhance soil fertility and enhance immunity of grape plants, the disease and pest resistance of the grape plants is improved, and therefore the fruit yield is improved.