Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for extracting apple polyphenol by using cutinase, and application thereof

A technology of apple polyphenols and cutinase, which is applied in the direction of pharmaceutical formulations, medical preparations containing active ingredients, anti-toxins, etc., can solve the problems of long extraction time and low extraction efficiency, and achieve abundant resources, broad market prospects, and obstacles The effect of UV absorption

Inactive Publication Date: 2011-11-09
JIANGNAN UNIV
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2008, researchers reported the method of extracting polyphenols in apple pomace by using pectinase, but the extraction time of this method was too long and the extraction efficiency was low.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Crush the dried apple peel, and put it in an aqueous solution at 37°C, according to the ratio of solid to liquid (1:75 (g / ml), the ratio of enzyme substrate (m / m) to 1:100, the reaction pH to 8.0, and the reaction time to 20 minutes. The extraction rate of polyphenols was 60.23%.

Embodiment 2

[0019] Crush the dried apple peel, and put it in an aqueous solution at 60°C, according to the ratio of solid to liquid (1:75 (g / ml), the ratio of enzyme substrate (m / m) to 1:100, the reaction pH to 8.0, and the reaction time to 20 minutes. The extraction rate of polyphenols was 73.35%.

Embodiment 3

[0021] Grind and dry apple peel, in 20% ethanol system at 50°C, according to the ratio of solid to liquid 1:50 (g / ml), enzyme substrate ratio (m / m) 1:100, reaction pH 8.0, reaction time 15min. The extraction rate of polyphenols was 80.70%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for extracting apple polyphenol by using cutinase. The cutinase has the following characteristic that: the cutinase can be provided for catalyzing cutin hydrolysis in an aqueous solution or an ethanol solution. With the present invention, the cutinase is provided for producing the apple polyphenol through adopting the characteristic of the cutinase. According to the present invention, the apple polyphenol can be extracted at a low temperature through a assistance of the cutinase, organic solvents are not required or consumptions of the organic solvents are reduced such that a more green environmental-protection and energy-saving production for the apple polyphenol is provided.

Description

technical field [0001] The invention relates to a method for extracting apple polyphenols from apple peels, which involves extracting apple polyphenols from apple peels by using cutinase in a water or alcohol-containing system. Background technique [0002] Apple polyphenols have many important physiological functions and are an important class of natural bioactive substances. Due to the functions of anti-aging, anti-cancer, anti-oxidation, scavenging free radicals, preventing vitamin deterioration, anti-radiation and hindering ultraviolet absorption, apple polyphenols are used in medicine, food, cosmetics and other fields. Previous studies have shown that the polyphenol content in apple peel is much higher than other parts of apple. However, during the production of apple juice, cider, etc., apple pomace is produced. Some of these skin residues are buried directly as garbage, causing pollution to the environment; some are used to produce feed, and the protein content in ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/73A61P35/00A61P39/06A61K131/00
Inventor 王淼王莉
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com