Method for extracting apple polyphenol by using cutinase, and application thereof

A technology of apple polyphenols and cutinase, which is applied in the direction of pharmaceutical formulations, medical preparations containing active ingredients, anti-toxins, etc., can solve the problems of long extraction time and low extraction efficiency, and achieve abundant resources, broad market prospects, and obstacles The effect of UV absorption

Inactive Publication Date: 2011-11-09
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2008, researchers reported the method of extracting polyphenols in apple pomace by using pectinase, but the extraction time of this method was too long and the extraction efficiency was low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Crush the dried apple peel, and put it in an aqueous solution at 37°C, according to the ratio of solid to liquid (1:75 (g / ml), the ratio of enzyme substrate (m / m) to 1:100, the reaction pH to 8.0, and the reaction time to 20 minutes. The extraction rate of polyphenols was 60.23%.

Embodiment 2

[0019] Crush the dried apple peel, and put it in an aqueous solution at 60°C, according to the ratio of solid to liquid (1:75 (g / ml), the ratio of enzyme substrate (m / m) to 1:100, the reaction pH to 8.0, and the reaction time to 20 minutes. The extraction rate of polyphenols was 73.35%.

Embodiment 3

[0021] Grind and dry apple peel, in 20% ethanol system at 50°C, according to the ratio of solid to liquid 1:50 (g / ml), enzyme substrate ratio (m / m) 1:100, reaction pH 8.0, reaction time 15min. The extraction rate of polyphenols was 80.70%.

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PUM

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Abstract

The invention discloses a method for extracting apple polyphenol by using cutinase. The cutinase has the following characteristic that: the cutinase can be provided for catalyzing cutin hydrolysis in an aqueous solution or an ethanol solution. With the present invention, the cutinase is provided for producing the apple polyphenol through adopting the characteristic of the cutinase. According to the present invention, the apple polyphenol can be extracted at a low temperature through a assistance of the cutinase, organic solvents are not required or consumptions of the organic solvents are reduced such that a more green environmental-protection and energy-saving production for the apple polyphenol is provided.

Description

technical field [0001] The invention relates to a method for extracting apple polyphenols from apple peels, which involves extracting apple polyphenols from apple peels by using cutinase in a water or alcohol-containing system. Background technique [0002] Apple polyphenols have many important physiological functions and are an important class of natural bioactive substances. Due to the functions of anti-aging, anti-cancer, anti-oxidation, scavenging free radicals, preventing vitamin deterioration, anti-radiation and hindering ultraviolet absorption, apple polyphenols are used in medicine, food, cosmetics and other fields. Previous studies have shown that the polyphenol content in apple peel is much higher than other parts of apple. However, during the production of apple juice, cider, etc., apple pomace is produced. Some of these skin residues are buried directly as garbage, causing pollution to the environment; some are used to produce feed, and the protein content in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/73A61P35/00A61P39/06A61K131/00
Inventor 王淼王莉
Owner JIANGNAN UNIV
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