609 results about "White rot" patented technology

The invention relates to a bio-protein feed prepared by adopting a cellulose raw material, and the bio-protein feed provided by the invention comprises an organic material and complex bacteria, wherein the weight of the complex bacteria is 1-10% of that of the organic material; and the organic material comprises the cellulose raw material, rapeseed meal and bran; the cellulose raw material is one or more of corn stalks, corn cobs, straw, manioc waste and fruit residue; and the complex bacteria comprises bacillus subtilis, microzyme, lactobacillus and cellulose decomposition bacteria, and the cellulose decomposition bacteria is one or more of trichoderma harzianum and white-rot fungi. In the bio-protein feed, the content of probiotics is more than 108cfu/g, the bio-protein feed contains a variety of prolease, cellulase, amylase and the like, the preparation process is simple, and the fermentation period is short. The invention further discloses a preparation method of the feed.

A method for treatment of liquid waste is disclosed that includes the steps of (a) submitting the liquid waste to a pretreatment and (b) submitting the pretreated liquid waste to the action of fungi or active agents thereof. In particular, the described process is useful for the effective decoloration and detoxification of dye-containing liquid wastes using white rot fungi or active agents thereof.

A marine-derived Bacillus velezensis (Bacillus velezensis) BMF 03 having a deposit number of CCTCC NO: 2017374 is disclosed. BMF 03 strain and its aseptic fermentation broth have inhibitory effect onplant pathogenic fungi, including apple rot fungi, grape white rot fungi, mango anthracnose fungi and so on. The aseptic fermentation broth of BMF 03 strain can be used as an active ingredient to prepare an antibacterial drug, and the bacteria is selected from the group consisting of Keratinobacteria, Bacillus subtilis and Ralstonia solanacearum. The invention also discloses a fermentation methodof aseptic fermentation broth of BMF 03. The invention adopts a plate confrontation method and an Oxford cup method to screen a new marine bacterial strain BMF-03 with strong bacteriostatic effect andwide bacteriostatic spectrum, and adopts strain morphological observation, physiological and biochemical tests and 16S rDNA sequence analysis to determine the species relationship of excellent resistant strains. At that same time, the bacteriostatic substance produce by the strain, the sporulation fermentation medium and the shake flask fermentation conditions were also study, and the promoting effect of the strain fermentation broth and the solid fermentation product on the growth of the cucumber seedling was clarified, which laid a foundation for the application of the strain.

A method of amplification culture of microorganism for waste water treatment. The waste water comprises heterocyclic compounds, polycyclic aromatic hydrocarbons, cellulose and/or lignin. The method comprises the following steps: adding waste water containing heterocyclic compounds, polycyclic aromatic hydrocarbons, cellulose and/or lignin into an acclimation reactor; adding a nutritional agent, acomplex bacterial agent, an enzyme preparation, a citric acid and riboflavin on a basis of concentrations in the waste water; performing aeration of the waste water, adjusting the liquid level and temperature of the acclimation reactor, holding for a period of time to allow the microorganism in the complex bacterial agent to reach a logarithmic growth phase, wherein on a basis of concentrations in the waste water, the complex bacterial agent comprises pseudomonas spp. microorganism, bacillus sp. microorganism, and white rot fungi, and the enzyme preparation comprises protease, amylase, cellulose and lipase. The invention also provides a method of waste water treatment by using the microorganism to be amplified and cultured.

The invention relates to technology for preparing biogas by degrading coal with microorganisms. The technology is suitable for any environment with oxygen or without oxygen. Under the condition of room temperature (15 to 35 DEG C), biologic methane can be ensured to be continuously generated without activating a strain in a way of raising temperature, so the development cost is effectively reduced; the adopted white-rot fungi is highly adaptive to the environment and is low in cost, so the technology has the feasibility of project development; and different types of colonies can be more effectively used and the effect of the colonies can be improved, so the degradation rate of a coal body is increased, and the yield of biological methane is improved.

The invention discloses a method for treating malachite green dye waste water, which comprises the following steps of: preparing a bacterial cellulose membrane; modifying the bacterial cellulose membrane; immobilizing white rot fungi by using the modified bacterial cellulose membrane; adding the obtained immobilized white rot fungi modified bacterial cellulose membrane into 50 mL of the malachite green dye waste water; dissolving 20 to 40 mg of malachite green into 1 L of white rot fungi liquid restrictive culture medium; treating the waste water on a gas bath constant-temperature shaking table at a speed of 120 rpm for 5 days at 28 DEG C, and measuring the chroma of the waste water at intervals of 24 hours; 5 days later, discarding the treated waste water, and retaining the immobilized fungi; adding 50 mL of newly-prepared dye waste water with the same concentration, treating the waste water under the same condition, and measuring the chroma of the waste water at intervals of 24 hours; and taking 5 days as a cycle, and repeating the steps for 5 times, wherein the removal rate of the chroma of the waste water is always over 86 percent. In the method, the referred bacillus xylinus and the white rot fungi are purchased from the China General Microbiological Culture Collection Center.

The invention relates to a straw feed fermented by white rot fungus and a preparation method thereof, and strain is the combination of white rot fungus Tf1 (pleurotus sajor-caju) and JG1 (pleurotus cornucopiae) which are screened in an oriented way, so that solid fermentation fungicide is cultured and prepared by three-stage propagation. Straw is pretreated by quicklime instead of being processed by high temperature sterilization without being washed by water or neutralized by acid. The obtained straw is accessed with white rot fungus composite fungicide to be fermented for 15-20 days, thus being taken as a first step of fermentation for degrading lignin in the straw and improving the straw digestion utilization rate of ruminant. After that, the fermented straw is accessed with yeast to be fermented for 3-4 days, thus being taken as a second step of fermentation for improving the crude protein content of the straw and further enhancing the nutritional value of the straw feed. After the two steps of fermentation, the crude fiber content of the straw is reduced by over 25%, the dry matter rumen (nylon bag technique) digestion rate is improved by over 20%, the crude protein content is increased by more than 50%, and the straw feed has no safety risk when being fed for animals. The invention has the characteristics of no energy consumption, no pollution, low cost, easy operation, high feeding value of the straw, and being suitable for local production of villages in China.

The invention discloses a lignin degradation solution and a preparation method thereof as well as a method for degrading lignin by using the lignin degradation solution, and belongs to biochemical and bio-refinery methods to solve the problem that the efficient biodegradation of lignin is difficult to realize by adopting single ligninase of laccase at present. The lignin degradation solution is prepared by the following steps: dissolving laccase and manganese peroxidase into an acetic acid-sodium acetate buffer solution of which the pH value is 4-6 in an enzyme load ratio of (10:1)-(1:5) to ensure that the enzyme loads of the laccase and manganese peroxidase are respectively 1U/ml-50U/ml and 1U/ml-50U/ml; and then adding 1-10mM of MnSO4 and 0.1-1mM of H2O2, wherein the laccase and manganese peroxidase are obtained by fermenting white rot fungi to obtain extracellular crude enzyme liquid and then performing separation and purification respectively. According to the lignin degradation solution and the methods disclosed by the invention, the synergistic oxidative degradation of macromolecular lignin with rich structural diversity is realized, and compared with a degradation reaction system with single ligninase, the degradation rate of the macromolecules of the lignin can each 30-50%, the degradation conversion efficiency is significantly improved, and the degradation solution and the methods can be applied to the fields of bio-refinery of lignocelluloses, biological pulping or environmental treatment and the like.

The invention discloses a micro-ecological flora of crop root system probiotics and an application thereof. The micro-ecological flora comprises a leading flora, a balance flora, a basic flora, a secondary-function flora and a special-function flora, wherein the leading flora comprises 25-35 parts of nitrogen-fixing bacteria by mass; the balance flora comprises 17-23 parts of phosphate solubilizing bacteria and 17-23 parts of potassium solubilizing bacteria by mass; the basic flora comprises 2-7 parts of yeast, 2-7 parts of rhizobia and 2-7 parts of photosynthetic bacteria by mass; the secondary-function flora comprises 2-7 parts of bacillus and 2-7 parts of actinomycetes by mass; the special-function flora comprises 2-4 parts of white rot fungi and 1-3 parts of pseudomonas. In the micro-ecological flora disclosed by the invention, the strain stability is good, functional microorganisms can be effectively planted in soil, and the functions of improving the crop yield and quality, improving the soil, detoxifying heavy metals and resisting diseases and pests can be realized.

The invention discloses a wheat straw decomposition agent and a method for preparing the same. The wheat straw decomposition agent and the method have the advantage that bacillus subtilis, trichoderma viride, aspergillus niger, white rot fungi and candida are used as production strains, are free of antagonism and easy to cultivate and are nontoxic and harmless, and excellent wheat straw degradation effects can be realized; the method for preparing the wheat straw decomposition agent is simple, the strains are easily available and are convenient to store and low in cost, and obvious decomposition effects can be realized; the yield of crops can be greatly increased by decomposed wheat straw organic fertilizers, and the quality of the crops can be greatly improved by the decomposed wheat straw organic fertilizers.

The invention discloses a grass fiber bio-separation compound preparation and an application method thereof. The grass fiber bio-separation compound preparation comprises bacteria components and enzyme components, wherein the bacteria components comprise white rot fungi and zymogeneous bacteria, and the enzyme components comprise cellulose, xylanase, laccase, protease, thermostable amylase, ligninase and pectinase. According to the grass fiber bio-separation compound preparation, straw serving as the raw material is treated with a biological method, a biological cycle liquid and a waste cooking liquid produced in the treatment process can be recycled without drainage, and lignin can be extracted; and besides, according to the method, straw does not require cutting and dust removal, and the cooking does not require high temperature and high pressure, so that a large quantity of manpower and electric charges is omitted, and the preparation and the method are suitable for a plurality of raw materials such as wheat straw, straw, cotton stalks, reeds and the like, can be used for producing a paddle board, molded pulp, various types packaging paper, office paper and household paper and have the advantages of low cost and environment protection.

The invention discloses compound bactericide for treating sewage and a sewage treatment method thereof, and belongs to the technical field of sewage treatment. The compound bactericide comprises the following active ingredients from following strains: rhodopseudomonas, anaerobic ammonium oxidation bacteria, candida utilis, thiobacillus denitrificans, white-rot fungi, lactobacillus plantarum, bifidobacterium, lactobacillus acidophilus, green nonsulfur bacteria, phosphorus-accumulating bacteria, aspergillus oryzae, nitrosomas, streptomyces, bacillus subtilis and propionibacterium acidipropionici. The invention also provides the method using the compound bactericide to treat the sewage. The compound bactericide and the sewage treatment method have the advantages that the effect of treating sewage is better, the technology is simple, the implementing is easy, the operability is strong, the pollution to environment is avoided, the industrial requirements are met, and the application prospect is better.

The invention discloses a bacillus amyloliquefaciens YJ01 which is preserved in China General Microbiological Culture Collection (CGMCC) center of China Committee for Culture Collection of Microorganisms and the preservation number of the bacillus amyloliquefaciens is CGMCCNO.7328. The bacillus amyloliquefaciens is mainly used for preventing and treating a fungal disease of a wine-brewing grape. The bacillus amyloliquefaciens YJ01 can metabolize out antibacterial substances iturin A and surfactin, thereby playing an obvious role in inhibiting various pathogenic fungi of the wine-brewing grape. The bacillus amyloliquefaciens has the advantages of being high in culture proliferation speed, capable of being cultured artificially, easy to operate, convenient to produce and apply, strong in adversity resisting capability, and the like; and the bacillus amyloliquefaciens has significance for preventing and treating diseases such as gray mold, white rot and anthracnose of wine-brewing grape caused by fungi, and is simple in culturing conditions, easy to preserve and easy to produce industrially, thereby having good development and application prospect.

The invention discloses a method for removing 2,4-dichlorophenol in water by using polyurethane sponge fixed white rot fungi, belonging to the field of environmental microbiology. The method comprises the following steps: preprocessing a carrier, preparing fixed white rot fungi and removing 2, 4-dichlorophenol in water. The carrier has the characteristics of porosity, hydrophily and good adsorbability, enjoys stable chemical property and hydrolytic resistance, high organism bearing capacity and mass transfer rate and is not soluble in acid/alkaline water solution; while the fixed carrier can effectively absorb and fix white rot fungi, improve capacity of fixed white rot fungi in removing 2,4-dichlorophenol in water and leads the fixed white rot fungi to be easily separated from the product, thus being applicable to execute biological reaction processing on multiple sewages.

The invention discloses antibacterial packaging paper. A preparation process comprises: preparation of packaging paper, preparation of a waterproof agent coating, and preparation of an antibacterial agent coating. According to the antibacterial packaging paper disclosed by the invention, the processing raw materials are bamboos and waste timbers. As bamboo fiber is naturally antibacterial, timbers are replaced by bamboos, so that cutting of trees is avoided, the environment is protected, the ecological balance is maintained, waste is turned into wealth as waste timbers are used, and the cost is saved; the bamboo fiber and wood fiber are matched in use, so that the strength and flexibility of paper are remarkably improved and the packaging paper is wide in using range; the waterproof layer and the antibacterial layer are natural materials which do not harm human body and environment and play natural roles of preventing water and resisting bacteria; in a delignification process, the raw materials are pre-treated by using white rot fungi first, so that the time and dosage for delignification by hydrogen peroxide are reduced; hydrogen peroxide further can disinfect and bleach the packaging paper, is safe and non-toxic, and does not cause the environmental pollution, thereby killing two birds with one stone and saving the cost.

The invention belongs to the technical field of pesticide and relates to a pesticide for preventing and treating fruit diseases such as strawberry anthracnose, powdery mildew and root rot disease, grape white rot and powdery mildew, pear scab and the like. The pesticide comprises the active compositions of ethylicin and pyrachostrobin with the weight ratio of 1-50:1-20, and preferably 10-40:1-15. The invention has the advantages that firstly, the drug resistance caused by long-term use of single preparation is avoided, and the effective time of pesticide is prolonged; secondly, the combination of the ethylicin and the pyrachostrobin has a better synergistic effect, and the application amount and the cost are reduced; thirdly, the pesticide improves the treating effect, widens the anti-fungal spectrum, and has long effective duration and good effect on preventing and treating a plurality of diseases; and fourthly, the pesticide has low toxicity and non-pollution and is safe for environment.

The invention discloses a method for treating dye waste water by enzyme production through mixed biomass fermenting. In the method, white rot fungi which can generate a lignin degrading enzyme system are used as a fermenting enzyme-production strain; a solid and liquid culture method is adopted, a cottonseed hull and/or paddy straw are/is used as a fermentation substrate, and lignin degrading enzyme is induced to obtain an optimal lignin degrading enzyme system; and finally, crude enzyme liquid is extracted, separated and prepared and can be used for decoloring treatment of waste water of commonly-used synthetic dye, such as Congo red, phenol red, bromophenol blue, crystal violet, malachite green and the like so as to obtain the optimal decoloring effect of dye sewage. The method is simple and is easy to operate. The enzyme system induced by the method disclosed by the invention has high enzymolysis efficiency, and the optimal decoloring effect can be achieved by adopting the enzyme system to treat the dye waste water.

The invention discloses a wood preservative and a preparation method thereof and belongs to the field of preservatives. The invention aims to solve the technical problem that a wood preservative has severe environmental harm, low permeability and low erosion resistance in the prior art. The wood preservative is prepared from nano copper oxide, ammonium citrate, distilled water, chitosan and a solution of acetic acid. The preparation method comprises the following steps of: 1, mixing the nano copper oxide and the ammonium citrate, adding the distilled water, and stirring to obtain nano copper oxid dispersion; and 2, adding the chitosan into the solution of acetic acid, adding the nano copper oxid dispersion, and stirring to obtain the wood preservative. The wood preservative is black brown viscous liquid and has an obvious bacteriostatic effect on wood decomposing fungi, such as monilinia fructicola, namely Gloeophyllum trabeum, and white rot fungi, namely Coriolus versicolor.