32 results about "White rot" patented technology

The invention relates to the technical field of organic solid waste resourceful treatment, in particular to a method for enhancing degradation of biodegradable plastic compost based on white-rot fungi and manganese modified charcoal. According to the method, manganese modified charcoal and white-rot fungi are subjected to synergistic treatment, and the efficient method for intensively degrading the biodegradable plastic through aerobic composting is constructed. The method effectively overcomes the technical bottlenecks of slow degradation starting, long degradation period and easy generation of micro-plastic residues of the biodegradable plastic in traditional compost, and realizes rapid and thorough degradation of the biodegradable plastic. Besides, the method also promotes the conversion of humus precursor substances in the compost material and accelerates and stabilizes the synthesis of humus, so that the decomposition degree of the compost product is remarkably improved, and the quality and environmental safety of the final compost product are synchronously improved while the biodegradable plastic is efficiently degraded. And an efficient and reliable solution is provided for recycling of organic solid wastes and degradation of biodegradable plastic compost.

The application discloses a dye degradation system and method with rice straw as a carbon source, and belongs to the technical field of wastewater treatment. The application successfully constructs an economical combined degradation system by combining the ability of white rot fungi to decompose macromolecular organic matter and the characteristics of the power generation microorganism MR-1 to degrade pollutants in a broad spectrum and efficiently. The system uses rice straw as a carbon source, uses white rot fungi to degrade the rice straw, obtains a degraded straw solution, and then uses the power generation microorganism MR-1 to perform anaerobic respiratory metabolism on the degraded straw solution, releases electrons, and transmits the electrons to dye pollutants, so as to degrade dye wastewater. The combined degradation system can not only make up for the deficiency of the power generation microorganism MR-1 in utilizing macromolecular organic matter carbon sources, but also can significantly reduce the cost of carbon sources, and simultaneously realize the dual goals of wastewater treatment and solid waste resource utilization. The method is environmentally friendly, treats waste with waste, and has potential to be popularized and applied in practical application.

The invention discloses a method for pretreating biomass garbage and application. The method for pretreating the biomass garbage comprises the following steps: inoculating white-rot fungi into slurry containing the biomass garbage, and culturing the white-rot fungi. According to the method for pretreating the biomass garbage, the white-rot fungi are inoculated to the slurry of the biomass garbage for cultivation, so that unwanted microorganisms in the biomass garbage are killed, the biodegradability of the kitchen garbage is improved, traditional chemical and physical and chemical pretreatment is replaced, and therefore the energy cost is reduced.

The invention belongs to the field of resource utilization of livestock and poultry manure, and particularly relates to a thermophilic ammoniation compound microbial agent, a fertilizer and application. The thermophilic ammoniation compound microbial agent is prepared by compounding lysinibacillus spp., bacillus megatherium and white-rot fungi, the preservation number of the lysinibacillus sphaeroides is CCTCC NO: M 20242776, the preservation number of the bacillus megaterium is CCTCC NO: M 20242777, the preservation number of the white-rot fungi is CCTCC NO: M 20242778, and the three strains are all preserved in the China Center for Type Culture Collection. Tests prove that the thermophilic ammoniation compound microbial agent shows higher ammoniation activity at 60 DEG C, and can be used for promoting the compost recycling process and improving the compost product quality.

The invention relates to the technical field of solid waste treatment, and discloses a method for promoting white-rot fungi to degrade plastics by a pretreatment technology, which comprises the following steps: adding plastics into dimethylbenzene, heating and stirring until the plastics are completely dissolved, uniformly spreading on a glass plate, cooling, separating, air-drying to obtain a plastic film, and shearing the plastic film into a size of 30mm * 30mm to obtain an original polystyrene plastic film; an original polystyrene plastic film is put into a beaker for pretreatment, white-rot fungi degradation is performed after pretreatment, and the plastic degradation effect of the white-rot fungi is enhanced; the problems that in the prior art, plastic molecular chains cannot be efficiently decomposed, the degradation efficiency is low, the energy consumption is high, secondary pollutants can be generated in the degradation process, and engineering application is difficult to achieve are solved.

The invention provides a phenolic acid degradation fertilizer and a preparation method thereof, raw materials comprise metal cross-linked gel and engineering bacterium spores coated with the metal cross-linked gel, the engineering bacterium spores are one or more of white-rot fungus spores, trichoderma harzianum spores and aspergillus aculeatus spores, and the metal cross-linked gel comprises ferric chloride, sodium alginate and gelatin. Iron chloride, sodium alginate and gelatin form metal cross-linked gel to coat the engineering bacterium spores, phenolic acid pollutants in the environment can chelate metal ions in the metal cross-linked gel, and the engineering bacterium spores in the phenolic acid degradation fertilizer can be released only when the concentration of phenolic acid in the environment reaches a certain threshold value. Further, phenolic acid in the environment is decomposed, and it is ensured that all activity of functional bacteria is delivered to a phenolic acid enriched rhizosphere pollution area.

The invention belongs to the technical field of microorganisms, and particularly relates to grape endophytic fungi NZ1-b-1 and application thereof in prevention and treatment of grape diseases. The grape endophytic fungus NZ1-b-1 is classified and named as subclavian endophytic fungus and is preserved in the China Center for Type Culture Collection on July 14, 2025, and the preservation number of the grape endophytic fungus NZ1-b-1 is CCTCC (China Center for Type Culture Collection) NO: M 20251604. The grape endophytic fungi NZ1-b-1 provided by the invention are endophytic fungi separated from grape leaves, the culture method is simple, the cost is low, and the separation method is easy to operate. The grape endophytic fungus NZ1-b-1 has an antibacterial effect on grape botrytis cinerea and white rot fungi, has a relatively high prevention and treatment effect on grape gray mold and white rot, has a certain prevention and treatment effect on grape downy mildew, and has the potential of being developed into a broad-spectrum antibacterial agent for grape fungal diseases.

This invention relates to the field of microbial technology. It provides a culture medium for regulating a single nitrogen source in white-rot fungi, its preparation method, and its applications. The culture medium uses water as a solution and further includes potassium dihydrogen phosphate, disodium hydrogen phosphate, a carbon source, magnesium sulfate heptahydrate, manganese sulfate tetrahydrate, zinc sulfate heptahydrate, copper sulfate pentahydrate, calcium chloride heptahydrate, ferrous sulfate heptahydrate, and a nitrogen source. The core advantage of the culture medium and preparation method designed in this invention lies in the precise control of the type of nitrogen source, effectively eliminating interference from multiple nitrogen sources. This provides a dedicated culture medium for studying the physiological metabolism, molecular mechanisms, and biosynthesis of white-rot fungi under specific nitrogen source environments, and is widely applicable to transcriptional, protein and metabolic regulation, as well as biodegradation and synthetic biology in white-rot fungi.

The invention discloses a strain of schizophyllum commune DKK and application thereof in prevention and treatment of rice and wheat diseases, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation date is May 19, 2025, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.42010. The DKK strain is separated from persimmon fruits on which sporocarp grows. Researches show that the strain DKK inhibits the growth of rhizoctonia solani through antagonism and hyperparasitism; and the compound also has relatively good antagonistic activity on other pathogenic bacteria such as cochliobotrys miyabeanus, fusarium graminearum, wheat stem rot pathogen, potato late blight pathogen, tomato fusarium wilt pathogen, sclerotium fistulosum, strawberry botrytis cinerea, colletotrichum gloeosporioides and ligustrum japonicum leaf spot pathogen.

This invention provides a solid-state fermentation inoculant, its preparation method, and its application. The preparation method of the solid-state fermentation inoculant involves inoculating *Phanerochaete chrysosporium* into a seed carrier and culturing it in a sterile, pure oxygen environment. Under pure oxygen conditions, the inoculated *Phanerochaete chrysosporium* exhibits a significantly increased reproduction rate, forming a high-density microbial community. Simultaneously, the continuous and stable nutrient supply from the seed carrier enables the inoculant to quickly establish a competitive advantage upon entering materials containing other microorganisms. By seizing nutrients and oxygen resources, it inhibits the growth and reproduction of other microorganisms, ultimately increasing the abundance of white-rot fungi in the fermented product, thereby enhancing the degradation efficiency of petroleum hydrocarbons in the soil. Compared to traditional solid-state fermentation methods, the inoculant production process is very simple and low-cost. The solid-state fermentation inoculant product of this invention has a high microbial density and a relatively large number of target strains, resulting in higher efficiency in soil pollution remediation and increased soil fertility.

The invention discloses nicotine-resistant irpex laceratus IL01 and application thereof, and belongs to the technical field of agricultural microorganisms. The invention provides a tobacco source strain with the fermentation characteristics of nicotine resistance, salt resistance, acid resistance, high yield of lignocellulase activity, aroma production and the like, the tobacco source strain is identified as white-rot fungus irpex laceratus, on one hand, different substrates can be fermented by the strain to prepare tobacco flavors, and the sensory quality of cigarettes can be improved after the cigarettes are perfumed; on the other hand, tobacco source lignocellulose such as stem paste, cut stems, tobacco leaves, tobacco powder, tobacco soil sticks or tobacco straw can be utilized in a high-valued mode and converted into flavor substances. According to the method, a new technical approach can be provided for improving the quality of cigarette products and reducing the cost, a new thought is provided for tobacco biomass resource utilization in the future, the method conforms to the double-carbon development concept, and the method has important significance for promoting agricultural green and low-carbon development and comprehensively achieving rural revitalization.

The invention discloses a preparation method and application of high-cohesiveness environment-friendly antibacterial woodworking glue. The woodworking glue is a water-based emulsion, and is prepared through the processes of prepolymerization of polyisocyanate and polyol, chain extension of a hydrophilic monomer, a chain extender and a silane coupling agent, end capping and neutralization of an imidazole compound, emulsification and post-chain extension. The adhesive developed by the invention takes water as a dispersion medium, does not contain formaldehyde, is low in VOC (Volatile Organic Compounds), and is safe and environment-friendly. A molecular side chain of the adhesive contains hydrolyzable siloxane, a terminal group contains thermal reactive blocked isocyanate, and when the adhesive is applied, self-crosslinking of adhesive molecules can be realized, and covalent bonds can be formed with hydroxyl groups on the surface of wood, so that the adhesive has high cohesive force and strong interface adhesion. A hydrophobic structure formed by siloxane chain segments can further improve the hydrolysis resistance of the adhesive. Besides, the glue system has a cationic characteristic, and the imidazole compound released by curing and the side chain tertiary amine salt have a synergistic effect, so that breeding of microorganisms such as white-rot fungi and the like can be efficiently inhibited. The adhesive is suitable for bonding wood materials, can be heated and cured after being glued and attached, and has good application value in manufacturing of various wood products.

The invention belongs to the technical field of bottom mud repair, and particularly relates to a composite bottom mud repair agent as well as a preparation method and application thereof. The composite bottom mud repairing agent comprises a main functional component, an auxiliary functional component and a stabilizer, the main functional components comprise modified attapulgite, a compound microbial agent and nano-hydroxyapatite; the auxiliary functional components comprise straw carbon, an adhesive and humate; the modified attapulgite is obtained by modifying attapulgite with an acid solution and a surfactant; the compound microbial agent comprises pseudomonas, bacillus, white-rot fungi, compound nitrifying bacteria and phosphorus-accumulating bacteria. Through the modified attapulgite, the compound microbial agent and the nano-hydroxyapatite, and in combination with other components, heavy metals, organic pollutants and organic nitrogen and phosphorus in the polluted bottom mud can be efficiently removed; the microenvironment and structure of the bottom mud can be improved, and the environmental adaptability is improved.

The invention discloses an enzymatic biological degumming method of industrial hemp fiber, and relates to the field of degumming of industrial hemp fiber, according to the enzymatic biological degumming method of industrial hemp fiber, MnP enzyme is produced by white rot fungi, veratryl alcohol is added as a mediator, and the MnP enzyme and the veratryl alcohol form an oxidation-reduction cycle; and colloid components such as lignin-hemicellulose compounds in the raw industrial hemp can be efficiently degraded. Compared with the prior art, the residual gum rate is greatly reduced to 1.2%-1.8% and is reduced by 60%-85% compared with a contrast, the degumming rate is larger than or equal to 98%, and the degumming effect is quite remarkable. Meanwhile, ultrasonic pretreatment destroys a wax coat on the surface of the fiber and promotes enzyme liquid permeation, and mild biological catalysis conditions are combined, so that damage to the fiber is reduced to the maximum extent. Due to the series of advantages, the degummed industrial hemp fibers are excellent in key indexes such as strength and whiteness, and completely meet the quality requirements of high-end textile products.

The invention relates to the technical field of microbial remediation, in particular to a microbial complex microbial inoculant for remediation of petroleum hydrocarbon contaminated soil and a remediation method and application of the microbial complex microbial inoculant. The complex microbial inoculant is composed of sulfate reducing bacteria, methanogens, oleophilic bacteria and white-rot fungi. The ratio of the sulfate reducing bacteria to the methanogens to the oleophilic bacteria to the white-rot fungi is (2-4): (0.5-2): (3-5): (1-3) according to inoculation biomass. The remediation method comprises the following steps: inoculating bacterial liquid of sulfate reducing bacteria and methanogens to petroleum hydrocarbon contaminated soil below a certain depth, and continuously remediating for 2-5 weeks; the surface layer of the contaminated soil is inoculated with bacterial liquid of oleophilic bacteria and white-rot fungi, and continuous remediation is conducted for 2-5 weeks. According to the method, a strategy of anaerobic-aerobic two stages and fungus-bacteria transboundary synergy is utilized, refined remediation of a polluted site is realized, and finally, the complex and stubborn petroleum hydrocarbon pollutants are fully degraded by utilizing a mutual synergy effect after multiple microorganisms are compounded.

The invention discloses a diversified regeneration method of an inactivated SCR (Selective Catalytic Reduction) denitration catalyst, which comprises the following steps: firstly, carrying out apparent inspection, performance test and comprehensive analysis on the inactivated catalyst to determine the variety, distribution and degree of toxic substances; next, physical, chemical, thermal regeneration and bioremediation are carried out in this way. Surface and pore channel blockages are removed through physical repairing; chemical remediation parameters are determined according to poisoning types; grading roasting is carried out in thermal regeneration in an inert atmosphere, and parameters are determined according to poisoning degrees; in the bioremediation process, white-rot fungi are used for degrading organic toxicants. A poisoning type identification and evaluation system is established, different poisoning mechanisms are clarified, and a basis is provided for repair. Multiple means are integrated, and targeted, efficient and overall activity recovery is achieved. The method emphasizes grading restoration under precise diagnosis, reduces energy consumption and agent dosage, promotes regeneration to develop towards the environment-friendly, economic and sustainable direction, provides a practical and green scheme for the industry, and assists emission reduction and environmental protection.

The invention relates to the technical field of solid waste treatment, and particularly discloses a method for degrading a plastic film by using white-rot fungi, which comprises the following steps: inoculating the white-rot fungi into a solid matrix, then adding the plastic film to be degraded, and carrying out solid state fermentation at 30-37 DEG C for 40-50 days. After solid-state fermentation, white-rot fungi are effectively planted on the surface of the plastic film, and oxidative depolymerization and mineralization of the plastic film are promoted, so that degradation of the plastic film is realized.

The invention discloses a low-cost treatment method for improving long-acting mildew-proof and antibacterial properties of wood and bamboo materials, which comprises the following steps: S1, treating white rot fungi to the surface and the interior of the wood and bamboo materials, and then culturing; and S2, a mildew-proof antibacterial agent is treated into the wood and bamboo wood infected by the white rot fungi, and the long-acting mildew-proof modified wood and bamboo wood can be obtained. Compared with traditional enzyme catalysis modification, various enzyme molecules are generated in the bamboo wood, the stability of the enzyme is high, the accessibility to a mildew-proof antibacterial agent and catalytic substances of main components of the bamboo wood is high, the catalytic efficiency is high, and the enzyme catalysis cost is low. The white rot fungi are utilized to secrete multiple enzymes in the bamboo wood degradation process, the multiple enzymes cooperate to form a stable catalytic system, through regulation and control of the whole treatment process, the mechanical property of the wood and bamboo wood can be not reduced or even improved, and after modification treatment is conducted through the antibacterial agent, the hardness and the elastic modulus of the bamboo wood are remarkably improved compared with those of the bamboo wood not treated through the antibacterial agent.

The invention relates to the technical field of feed processing, and discloses a straw and Chinese herbal medicine compounded mutton sheep feed fermentation method which comprises the following steps: firstly, mixing crop straws and Chinese herbal medicine auxiliary materials, carrying out mechanochemical activation, inoculating a complex microbial inoculant, carrying out aerobic fermentation, and degrading lignin by using white-rot fungi and generating phenoxy free radicals; then, an oxidative coupling initiation solution prepared from manganese sulfate monohydrate, tannic acid and a citric acid buffer solution is sprayed to the materials, free radicals are induced to be subjected to a coupling polymerization reaction, and oxygen in gaps of the materials is rapidly consumed through a chemical path so as to establish an anaerobic environment; and finally sealing and carrying out anaerobic fermentation. The technical problem that physical compaction and oxygen removal of straw materials are not thorough is solved through a chemical oxygen consumption mechanism, the degradation rate of lignocellulose is remarkably increased through the synergistic effect of biological enzymolysis and chemical oxidation, phenolic acid antibacterial components in Chinese herbal medicine are effectively converted, dominant growth of lactic acid bacteria is guaranteed, and the method is suitable for industrial production. And the fermented feed with excellent quality and high digestibility is obtained.

The application discloses application of a porous carrier material in microbial immobilization and belongs to the technical field of microbial immobilization. a The porous carrier material has a positive surface (pK a 8) and moderate hydrophilicity (contact angle of 20-60 degrees), is used for immobilization of microorganisms, especially white rot fungi, and obtains a porous carrier-microorganism composite material, which realizes efficient organic matter degradation and stable activity of the immobilized white rot fungi in actual wastewater without pH adjustment, solves the technical problem that the white rot fungi can only be used under acidic conditions in the prior art, and provides a new idea for application of functional microorganisms and degradation of emerging pollutants.

The invention discloses a composting method based on a compound microbial agent and a light-biological synergistic effect, and belongs to the technical field of recycling of agricultural and forestry wastes and livestock manure. The compound microbial agent consists of the following strains: white-rot fungi accounting for 25-35% of the total viable count; the high-temperature actinomycetes account for 15-20% of the total viable count; nitrogen-fixing bacteria accounting for 25-30% of the total viable count; the auxiliary flora accounts for 10-20% of the total viable count; the total concentration of the microbial inoculum is (50-80) * 10 < 8 > CFU / g, and the microbial inoculum is embedded by using a sodium alginate-charcoal-montmorillonite composite microcapsule. 0.3-0.5% of riboflavin and 0.1-0.3% of ferrous sulfate heptahydrate are added according to the dry weight of the compost to serve as a photocatalytic system, and meanwhile 5-8% of phosphoric acid modified charcoal is added to fix nitrogen and preserve fertilizer. The light degradation reaction of the lignin can be initiated under a natural illumination condition. By adjusting the pile height and the pile turning frequency, uniform distribution of illumination is ensured, the lignin degradation rate is increased by 40-50%, and the nitrogen retention effect is outstanding.

The invention discloses a preparation method of a multifunctional wood-based electromagnetic shielding composite material based on a ligand anchoring interface, relates to a preparation method of an electromagnetic shielding material, and aims to solve the technical problem that the existing electromagnetic shielding material with Fe3O4 and conductive metal Ni loaded on wood is poor in corrosion resistance and antibacterial and mildew-proof performance. The method comprises the following steps: after delignification of poplar veneers, soaking the poplar veneers in a ferric salt mixed solution to prepare WVS / Fe3O4; then, chemical nickel plating is carried out, and WVS / Fe3O4 / Ni is obtained; and finally, placing the WVS / Fe3O4 / Ni / MOF in a mixed solution of Co < 2 + > and 2-methylimidazole to grow MOF, thereby obtaining the WVS / Fe3O4 / Ni / MOF. The composite material has a stable shielding effect in the frequency band of 8-12 GHz, the average electromagnetic shielding effectiveness reaches 74.42 dB, 99.99% of electromagnetic waves can be shielded, microbial growth is remarkably inhibited, particularly, the white rot fungus inhibition effect is outstanding, photothermal conversion can be achieved, and the composite material can be used in the fields of electronic equipment protection and building decoration materials.

This invention discloses a *Rummeliibacillus* sp. and its application, relating to the field of microbial technology. The *Rummeliibacillus* sp., named *Rummeliibacillus* JY-2-4R, was deposited on February 11, 2026, at the Guangdong Provincial Microbial Culture Collection Center, located at 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou, with accession number GDMCC No: 67853. This invention applies the strain to the preparation of preparations antagonistic to *Actinidia chinensis* black spot, *Actinidia chinensis* soft rot, *Actinidia arvensis* white rot, *Actinidia arvensis* soft rot, or *Actinidia arvensis* root rot. The *Rummeliibacillus* JY-2-4R of this invention is a potential biological agent possessing stress resistance, growth promotion, and broad-spectrum antibacterial activity, with broad development potential and good application prospects, addressing the problem of a lack of functional microorganisms for the biological control of kiwifruit and *Actinidia arvensis* diseases.

The invention discloses a method for promoting anaerobic fermentation of wastes by utilizing biogas slurry backflow, and belongs to the technical field of anaerobic fermentation of agricultural wastes. The method comprises the following core steps: mixing 3-5kg of crushed straws, 35-45kg of livestock manure and a microbial agent composed of white rot fungi and aspergillus niger, adding the mixture into a hydrolysis acidification pool, adjusting TS to 8-12%, and intermittently aerating; transferring 2.5-3.5 L of materials to an anaerobic tank every day, intermittently aerating the anaerobic tank, carrying out hydraulic retention for 30 days, discharging the same amount of materials every day, refluxing 80% of biogas slurry, and returning biogas residue compost to the field. According to the method, the daily average methane yield reaches 36271.58 mL, the COD removal rate is 85.47%, the lignocellulose content is reduced, biogas slurry discharge and ammonia nitrogen damage are reduced, and the fungicide cost is saved.

The invention discloses a white-rot fungus BF-1 capable of degrading high-lignin straws and application thereof, belongs to the technical field of microorganisms, and aims to solve the problem that the feed utilization rate is low due to the fact that internal nutritional ingredients of the high-lignin straws such as wheat straws and rice straws are tightly wrapped by acidic washing lignin. The coriolus versicolor BF-1 is separated and screened from a shiitake mushroom sample, the strain can secrete laccase, manganese peroxidase, lignin peroxidase and other key enzymes, the stubborn lignin structure in straw is effectively decomposed through the synergistic effect, the strain is prepared into a microbial inoculum after being cultured in a suitable culture medium, the microbial inoculum is applied to the preparation process of straw fermented feed, and the straw fermented feed has the advantages of being high in yield and good in application prospect. According to the present invention, the nutritional value, the palatability and the safety of the feed can be improved, the digestion and the absorption of the livestock on the nutrients can be promoted, the green high-value utilization of the straw resource can be achieved, the environmental pollution can be reduced, the sustainable feed source can be provided for the animal husbandry, and the important application prospect and the important promotion value can be provided.

The invention provides a method for synergistically degrading lignin through compound bacteria-compound enzyme and application, and relates to the technical field of biological fermentation feed. The preparation method comprises the following steps: mixing corn straw, caragana microphylla, salix mongolica, bran and soybean meal in proportion, crushing, sterilizing at 121 DEG C, regulating the water content and pH value, inoculating a complex microbial inoculum consisting of pandoraea, bacillus thiamine and white-rot fungi, spraying a complex enzyme preparation consisting of laccase, xylanase and manganese peroxidase, and performing staged fermentation at 45-60 DEG C to obtain the high-yield organic fertilizer. The method realizes efficient degradation of lignin, cellulose and hemicellulose, increases the content of crude protein and crude fat, improves the rumen fermentation condition and meat quality of mutton sheep, and breaks through the limitations of low degradation efficiency and poor adaptability in the prior art.

The invention discloses application of tartaric acid in prevention and treatment of fungal diseases of grapes, and belongs to the field of plant source bacteriostatic agents. According to the method, the tartaric acid solution is uniformly sprayed on the fruit surfaces and the leaf surfaces of the grapes, so that white rot, anthracnose and gray mold of the grapes can be effectively prevented and treated, and the result shows that the plant source tartaric acid has the activity of inhibiting white rot fungi, anthracnose fungi and botrytis cinerea; the compound can be used as an agricultural disease inhibitor or bactericide for preventing and treating grape white rot, anthracnose and gray mold, effectively reduces the use amount of chemical pesticides, has better biological safety, and has important application value and market prospect in the aspect of grape disease prevention and treatment.

The application discloses a strain of Burkholderia gladioli H02, a microbial agent, a microbial fertilizer and application of the strain. The strain has significant antagonistic effect on plant pathogenic fungi such as Sclerotium rolfsii and Fusarium oxysporum and has inhibitory effect on the growth of Echinochloa crusgalli. The strain has a preservation number of CGMCC No.35003. The application also provides a method for preventing and treating white rot by combining the strain H02 with a chemical pesticide, thifluzamide, for peanut crops. The Burkholderia gladioli H02, the microbial agent and the microbial fertilizer have important significance for control of grass damage, prevention and treatment of soil-borne diseases and reduction of agricultural chemicals and increase of efficiency.