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103 results about "White rot" patented technology

Microbial complex microbial inoculant and method for degrading lignocellulose

The invention relates to the field of microorganisms, and discloses a microbial composite inoculant and a method for degrading lignocellulose. The compound microbial agent comprises phanerochaete chrysosporium, aspergillus niger, trichoderma reesei, neurospora crassa and gloeophyllum trabeum. The phanerochaete chrysosporium is a white-rot fungus with strong performance, and can secrete a plurality of lignocelluloses at the same time. Aspergillus niger, trichoderma reesei, neurospora crassa and gloeophyllum trabeum are mainly used for secreting high-activity cellulase and hemicellulase. Destroying the lignocellulose structure needs the combined action of various lignocellulose enzyme systems, and after the five strains are mixed, the synergistic effect among the enzyme systems in the system can be remarkably enhanced, the lignocellulose structure is efficiently destroyed, and the degradation rate of the wood fiber raw material is increased.
Owner:CHINA PETROLEUM & CHEMICAL CORP +2

Magnetic carbon-loaded slow-release microbial agent as well as preparation method and application thereof

The invention discloses a magnetic carbon-loaded slow-release microbial agent which comprises an activated carbon carrier containing ferroferric oxide nanoparticles and a compound microbial bacterium composed of bacillus megatherium, bacillus mucilaginosus, white-rot fungi and pseudomonas, and the mass ratio of the compound microbial bacterium to the activated carbon carrier is (3-4): 30. The invention further discloses a preparation method and application of the magnetic carbon-loaded slow-release microbial agent. According to the magnetic carbon-loaded slow-release microbial agent disclosed by the invention, the activated carbon containing the ferroferric oxide nano-particles is used as a carrier to load the compound bacteria, so that the stability of the microbial bacteria is improved, the microbial bacteria can better resist the impact of the environment, the service life of the microbial bacteria is prolonged, and the slow-release effect of the microbial agent in the remediation of vegetable planting soil is achieved; the nutrient absorption of the vegetables is promoted.
Owner:NINGXIA UNIVERSITY

Method for enhancing degradation of biodegradable plastic compost based on white-rot fungi and manganese modified charcoal

The invention relates to the technical field of organic solid waste resourceful treatment, in particular to a method for enhancing degradation of biodegradable plastic compost based on white-rot fungi and manganese modified charcoal. According to the method, manganese modified charcoal and white-rot fungi are subjected to synergistic treatment, and the efficient method for intensively degrading the biodegradable plastic through aerobic composting is constructed. The method effectively overcomes the technical bottlenecks of slow degradation starting, long degradation period and easy generation of micro-plastic residues of the biodegradable plastic in traditional compost, and realizes rapid and thorough degradation of the biodegradable plastic. Besides, the method also promotes the conversion of humus precursor substances in the compost material and accelerates and stabilizes the synthesis of humus, so that the decomposition degree of the compost product is remarkably improved, and the quality and environmental safety of the final compost product are synchronously improved while the biodegradable plastic is efficiently degraded. And an efficient and reliable solution is provided for recycling of organic solid wastes and degradation of biodegradable plastic compost.
Owner:EAST CHINA NORMAL UNIV

Microbial combined bacteria and method for improving fertility of newly-cultivated land

PendingCN121086908AAgriculture tools and machinesFungiTrichoderma spiraleBacilli
The invention discloses microbial combined bacteria and a method for improving fertility of newly-cultivated land. The method comprises the following steps: combining every two of bacillus amyloliquefaciens WK1, white rot fungi NDM3-2 and trichoderma helichum HJR7 to form three combined bacteria; the three combined bacteria are applied to soil containing straw and lentinus edodes residues respectively, and a pot experiment (rice and white clover crop rotation) for two years is carried out with no addition of any bacteria as a contrast; analyzing the physical and chemical properties of the soil and the microbiological indexes of the soil; and according to an analysis result, the combination of the white rot fungi NDM3-2 and the trichoderma helichum HJR7 is confirmed to be an ideal scheme for improving the fertility of the newly-cultivated land.
Owner:ZHEJIANG FORESTRY UNIVERSITY

Burkholderia gladioli and application of burkholderia gladioli in prevention and treatment of grape diseases

The invention discloses burkholderia gladioli and application of the burkholderia gladioli in prevention and treatment of grape diseases, the classification name of the burkholderia gladioli is burkholderia gladioli, the burkholderia gladioli is preserved in China General Microbiological Culture Collection Center on April 1, 2024, the preservation number is CGMCC No.30228, and the address of the preservation unit is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The strain is wide in antibacterial spectrum, and has good antibacterial activity on common pathogenic bacteria in grape planting, especially on grape white rot and grape anthracnose. According to the research, through directional screening of grape rhizosphere soil microorganisms, biocontrol bacteria capable of efficiently antagonizing grape white rot bacteria are pre-obtained, and a basic material is provided for biological prevention and control of grape white rot.
Owner:SHANDONG ACAD OF GRAPE

Preparation method of fungal mycelium-based electrode material

The invention belongs to the technical field of biological materials, and particularly relates to a preparation method of a fungal mycelium-based electrode material. The porous carbon material is obtained through low-temperature carbonization by taking the phanerochaete chrysosporium mycelium of a mode strain of white-rot fungi as a carbon source and KOH as an activating agent, and is used as the negative electrode material of the lithium ion battery. The used carbon source phanerochaete chrysosporium mycelium has the following advantages of being fast in propagation, easy to obtain and high in prepared material strength, and having a natural net structure. According to the invention, the carbonization temperature is 500-900 DEG C, which is far lower than the carbonization temperature (2200-3000 DEG C) of the graphite negative electrode of the lithium ion battery. The mycelium-based derived carbon material which is high in specific surface area (2000-3000m < 2 > / g), developed in pore structure, excellent in lithium storage performance, high in theoretical specific capacity and good in cycling stability is prepared by adopting a low-cost, low-energy-consumption, simple and controllable method, and has a good application prospect in the aspect of electrochemical energy storage.
Owner:SHANGHAI SECOND POLYTECHNIC UNIVERSITY

Compost and preparation method thereof

The invention provides compost and a preparation method thereof, and relates to the technical field of organic fertilizers, the compost comprises the following components by weight: 900-1100 parts of crop straw, 200-300 parts of animal excreta, 100-200 parts of an organic auxiliary material, and 1.5-7.5 parts of a complex microbial inoculant; the complex microbial inoculants comprise lactic acid bacteria, bacillus licheniformis, thermophilic trichoderma, actinomycetes and white rot fungi. The preparation method of the compost mainly comprises the following steps: stacking the crop straws and the organic auxiliary materials layer by layer, and compacting; uniformly sprinkling animal excrement on each layer, and then inoculating a complex microbial inoculant; punching a hole in the top of the pile to monitor the moisture content in the pile in the composting process; pile turning treatment is carried out in the composting process. According to the invention, the complex microbial inoculants are adopted for compost maturing treatment, so that the maturing speed can be increased, the maturing effect can be improved, and the content of nutrient substances in compost is increased.
Owner:BEIJING OGG NICK BIOTECH CO LTD

Composite extract of ampelopsis grossedentata leaves as well as preparation method and application of composite extract

The invention relates to the field of natural flavone extraction, in particular to a composite extract of ampelopsis grossedentata leaves as well as a preparation method and application of the composite extract. The preparation method of the composite extract of the ampelopsis grossedentata leaves comprises the following steps: step 1, cleaning and crushing the silybum marianum, the ampelopsis grossedentata leaves, the pineapples and the longan to obtain an initial mixed plant; 2, mixing the initial mixed plant with water, and treating under an ultrasonic power condition to obtain an ultrasonic treatment substance; 3, treating the ultrasonic treatment object under a microwave condition to obtain a microwave treatment object; step 4, adding acetobacter aceti, bacillus subtilis and white rot fungi into the microwave treated material, and fermenting to obtain a fermented mixture; and step 5, centrifuging, and collecting supernate to obtain the composite extract of the ampelopsis grossedentata leaves. The composite extract of the ampelopsis grossedentata leaves can improve the extraction efficiency of flavonoid substances, and has remarkable anti-inflammatory and anti-oxidation effects.
Owner:GUANGZHOU SAIJIAN BIO TECH

Streptomyces LZZY-S3 and application thereof

The invention provides streptomyces LZZY-S3 and application thereof, and belongs to the field of microorganisms. The streptomyces LZZY-S3 provided by the invention enriches strains for biological control, and realizes the dual functions of plant pathogenic bacterium resistance and plant growth promotion. The streptomyces LZZY-S3 has a broad-spectrum fungus inhibition function and can be applied to plant pathogenic bacterium resistance; pathogenic bacteria include: European tea leaf disease pathogen, Botryosphaeria spp., Gibberella zeae pathogen, fusarium oxysporum pathogen, wheat root rot pathogen, rice seedling rot pathogen, pumpkin gummy stem blight pathogen, wheat stem rot pathogen, Trichothecium roseum, grape white rot pathogen, and Colletotrichum graminum pathogen. The pathogenic bacteria include: European tea leaf disease pathogen, Botryosphaeria spp. Wherein the inhibition rate of tea cake disease germs reaches 91.2%. The streptomyces LZZY-S3 can also be applied to plant growth promotion, preparation of nitrogen-fixing bacterial agents, preparation and production of ACC deaminase bacterial agents and the like.
Owner:LIUZHOU VOCATIONAL & TECHN COLLEGE

Microbial agent for efficiently decomposing corn straws in situ under low-temperature condition as well as preparation method and application of microbial agent

The invention provides a microbial agent for efficiently decomposing corn straws in situ under a low-temperature condition as well as a preparation method and application of the microbial agent, and belongs to the technical field of agricultural microorganisms. The microbial agent is prepared from white rot fungi, cold-resistant paenibacillus, myrothecium delavayi, pseudomonas sp. And rhodobacter spp.. The microbial agent provided by the invention can adapt to a low-temperature environment, and compared with other microbial combinations, the microbial agent provided by the invention can significantly improve the straw degradation efficiency, the straw degradation rate under a low-temperature condition reaches up to 41%, and accumulation of organic matters in soil is facilitated.
Owner:CENTER FOR AGRICULTURAL TECHNOLOGY NORTHEAST INSTITUTE OF GEOGRAPHY & AGROECOLOGY

Method for preparing dihydroquercetin from larch sawdust

The invention relates to the technical field of dihydroquercetin preparation, in particular to a method for preparing dihydroquercetin from larch sawdust, which comprises the following steps: S1, raw material pretreatment: selecting roots of larch sawdust as a raw material, and pretreating the raw material to obtain a base material; s2, strain preparation: the strain is prepared, and the strain is used for fermentation treatment of the base material. According to the method, the white-rot fungi can rapidly grow and secrete a large amount of enzymes for decomposing lignin substances, so that the decomposition efficiency of larch wood chips is improved, the yield of dihydroquercetin is increased, the culture medium composed of soybean sprout juice, glucose and amino acid provides rich carbon and nitrogen nutrient substances for the white-rot fungi, the growth and metabolism requirements of the white-rot fungi are met, and the yield of the white-rot fungi is increased. The larch sawdust can generate more enzymes to decompose substances in the sawdust and promote the generation of dihydroquercetin, so that the purity and the yield of dihydroquercetin prepared from the larch sawdust are effectively improved.
Owner:SANYUAN LIHUA BIO-TECH CO LTD

Method for promoting decay, preserving nitrogen and immobilizing heavy metals in organic solid waste compost

The invention relates to a method for promoting decay, preserving nitrogen and fixing heavy metals in organic solid waste compost, and belongs to the technical field of organic solid waste recycling. The compound additive is prepared by combining a compound fungicide of white-rot fungi and sulfur reduction geobacter with phosphate, and compost humification synergism, nitrogen retention and heavy metal immobilization are achieved through the synergistic effect of biodegradation and chemical passivation. The method specifically comprises the following steps: (1) preparing a compound fermentation inoculant; (2) uniformly mixing a compound fermentation inoculant with phosphate, and adding the mixture into the compost raw material; and (3) carrying out aerobic composting treatment. The organic solid waste compost can promote decomposition and protect nitrogen and fix heavy metals, realizes safe land utilization of compost products, and has a good application prospect.
Owner:FUJIAN NORMAL UNIV

Streptomyces albidoflavus strain SaTC2601 and application thereof

The invention discloses a streptomyces albidoflavus strain SaTC2601 and application thereof, and belongs to the field of biological control. According to the invention, a streptomyces albidoflavus strain SaTC2601 is separated from faeces of larvae of WSFC, and the preservation number of the streptomyces albidoflavus strain SaTC2601 is CGMCC (China General Microbiological Culture Collection Center) No.33741. The strain SaTC2601 has a very good prevention and treatment effect on peanut southern blight caused by sclerotium rolfsii at peanut roots, also has a prevention and treatment effect on soil-borne pathogenic bacteria such as fusarium graminearum, fusarium solani, fusarium moniliforme, fusarium oxysporum, rhizoctonia solani and streptomyces scabies, and further, the strain SaTC2601 also has a very good growth promoting effect. The strain becomes a precious resource for new drug development and agricultural disease prevention and control, and is expected to play a huge role in the aspect of soil disease prevention and control.
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI

Method for solid state fermentation of bagasse by using white rot fungi

The invention relates to the technical field of biology, in particular to a method for solid state fermentation of bagasse by using white rot fungi, which comprises the following steps: (1) activation and propagation of strains: pouring the white rot fungi into a PDA culture medium for activation and propagation; (2) raw material preparation: air-drying fresh bagasse, adding water to adjust the water content of the bagasse to 60-70%, and adjusting the pH to 4-6 with glacial acetic acid; and (3) preparing the bagasse feed, namely mixing the white rot fungi subjected to expanding culture according to 1-5% of the weight of an air-dried substance with bagasse of which the initial pH is adjusted to be 4-6, adding 1-5% of soluble sugar, and fermenting for 10-45 days to obtain the fermented bagasse feed. According to the white rot fungus fermented bagasse prepared by the method disclosed by the invention, the hemicellulose content of the bagasse can be reduced to 26.62%-22.45%, the lignin content can be reduced to 10.67%-8.66%, the crude protein content of the bagasse can be increased to 1.61%-2.12%, the lactic acid content can be increased to 26.57 mg / L-181.69 mg / L, and high-quality feed application of the bagasse can be realized.
Owner:GUANGXI UNIV

Preparation method and application of Chinese herbal medicine extract fertilizer for enhancing immunity

The invention relates to the technical field of agricultural biology, and particularly discloses a preparation method and application of a Chinese herbal medicine extract fertilizer for enhancing immunity, the preparation method comprises the following steps: S1, performing water extraction and alcohol precipitation extraction on Chinese herbal medicine raw materials to obtain a Chinese herbal medicine extracting solution and medicine residues; s2, crushing the obtained medicine residues, inoculating white-rot fungi or bacillus subtilis, and performing solid-state fermentation at the fermentation temperature of 25-35 DEG C for 5-7 days to obtain a fermentation product; s3, concentrating and drying the obtained Chinese herbal medicine extracting solution to obtain Chinese herbal medicine extract powder; s4, the obtained Chinese herbal medicine extract powder is mixed with humic acid, ferric salt or zinc salt is added, stirring reaction is conducted under the condition that the pH ranges from 5.0 to 6.5, and a Chinese herbal medicine-humic acid metal chelate is formed; chinese herbal medicine compound extraction and microbial solid fermentation are combined, and traditional waste medicine residues are converted into high-activity organic fertilizer carriers, so that efficient utilization of resources is realized, meanwhile, the immune induction function of the fertilizer is enhanced, and the disease resistance of crops is remarkably improved.
Owner:JIANGSU FENGHUA BIOLOGICAL TECH CO LTD

Dye degradation system and method using rice straw as carbon source

The application discloses a dye degradation system and method with rice straw as a carbon source, and belongs to the technical field of wastewater treatment. The application successfully constructs an economical combined degradation system by combining the ability of white rot fungi to decompose macromolecular organic matter and the characteristics of the power generation microorganism MR-1 to degrade pollutants in a broad spectrum and efficiently. The system uses rice straw as a carbon source, uses white rot fungi to degrade the rice straw, obtains a degraded straw solution, and then uses the power generation microorganism MR-1 to perform anaerobic respiratory metabolism on the degraded straw solution, releases electrons, and transmits the electrons to dye pollutants, so as to degrade dye wastewater. The combined degradation system can not only make up for the deficiency of the power generation microorganism MR-1 in utilizing macromolecular organic matter carbon sources, but also can significantly reduce the cost of carbon sources, and simultaneously realize the dual goals of wastewater treatment and solid waste resource utilization. The method is environmentally friendly, treats waste with waste, and has potential to be popularized and applied in practical application.
Owner:JIANGSU UNIV

Method for promoting corn straw compost heat production by using exogenous microbial agent

The invention relates to the technical field of microbial agents, in particular to a method for promoting corn straw composting heat production by utilizing an exogenous microbial agent, which comprises the following steps: crushing corn straws, and mixing with chicken manure to obtain a fermentation raw material; laying a first layer of fermentation raw materials on the waterproof bottom film in the fermentation pit, uniformly scattering mixed freeze-dried powder of bacillus cereus, bacillus smith, bacillus thermophilic denitrification soil bacillus, bacillus licheniformis and ureibacillus thermophilus, laying a second layer of fermentation raw materials, and uniformly scattering EM (effective microorganisms) freeze-dried powder; after a third layer of fermentation raw materials is laid, white rot fungi, chaetomium, trichoderma harzianum and aspergillus niger mixed freeze-dried powder is evenly scattered, and after each layer of fermentation raw materials is laid, water is sprayed to adjust the water content; a heat exchange pipeline is laid between the two layers of fermentation materials, air is inflated into the pipeline through an air blower in the fermentation process, the oxygen content is adjusted to be 5%-15%, and high heat yield and efficient degradation of lignin and cellulose are achieved through functional flora construction.
Owner:JILIN UNIVERSITY

Northeast black land newly-renovated dry land barrier layer reduction method based on soil conditioner

The invention discloses a northeast black land and dry land barrier layer reduction method based on a soil conditioner, and belongs to the technical field of soil improvement. According to the method, an organic-inorganic composite conditioner and a composite microbial agent have a synergistic effect, and a mechanical deep ploughing technology is combined, so that the problem of a barrier layer of the northeast black land and dry land is effectively solved. Wherein the organic-inorganic composite conditioner is formed by compounding humic acid, bentonite, biochar, calcium-based minerals and agricultural straw, and the composite microbial agent comprises two functional strains including phosphate solubilizing bacteria and white-rot fungi. According to the method, through the technical path of combining physical improvement and biological improvement, a straw resource utilization channel is innovatively constructed while barrier layer reduction is achieved, and the problem of environmental pollution caused by traditional straw incineration can be effectively relieved. The method has the advantages of being low in raw material cost, simple in preparation process, convenient and efficient to operate and the like, the improvement effect on the dry land barrier layer is remarkable through actual verification, and good ecological benefits and application and popularization value are achieved.
Owner:INSTITUTE OF ENVIRONMENT AND SUSTAINABLE DEVELOPMENT IN AGRICULTURE CAAS

Irpex lacteus protoplast genetic transformation method

The invention relates to the field of genetic transformation, in particular to a protoplast genetic transformation method of white-rot fungi Irriga lactea. According to the invention, the preparation and genetic transformation method of the white-rot fungus Irpex lacteus protoplast is established for the first time, the transformation efficiency is 6-7 transformants / [mu] g DNA, the transformation period is 1-2 weeks, the method has the characteristics of high transformation efficiency, short transformation time period and the like, and powerful support is provided for research work of gene functions and strain improvement of Irpex lacteus in the future.
Owner:INSTITUTE OF ANIMAL SCIENCES OF CHINESE ACADEMY OF AGRICULTURAL SCIENCES

A gene related to resistance to white rot of Dendrobium officinale and its application

The present invention discloses a gene related to resistance to white rot of Dendrobium officinale and its application, wherein the CDS sequence of gene Dof013141 is shown in SEQ ID NO.1. The present invention isolates and screens the Dof013141 gene. The overexpression of this gene in Dendrobium officinale successfully overcomes the environmental pollution and drug resistance problems caused by traditional chemical control methods, not only effectively reduces the dependence on chemical agents, but also provides a new technical solution for green and sustainable agricultural prevention and control. The application prospect of this gene is broad. In addition to its successful performance in Dendrobium officinale, it can also be extended to the disease-resistant breeding of other susceptible plants, further broadening its application in the field of agricultural production and plant protection. The present invention not only provides a strong guarantee for improving the yield and quality of Dendrobium officinale, but also opens up a new research direction for the development and utilization of plant disease-resistant genes, with great economic and social value.
Owner:NANJING NORMAL UNIVERSITY

Preparation method and application of biological flocculant

The invention discloses a preparation method and application of a biological flocculant, white-rot fungi is used as a treatment strain, the white-rot fungi are subjected to enrichment culture and domestication to prepare a microbial liquid, the microbial liquid is compounded with a magnetic substance, and finally the compound is loaded on cellulose modified montmorillonite to prepare the flocculant. According to the flocculant disclosed by the invention, nutrient substances in the medicinal plant processing wastewater are converted into proteins by using the white-rot fungi, so that the medicinal plant processing wastewater is treated, and the COD (Chemical Oxygen Demand), the chromaticity and the pH (Potential of Hydrogen) of the wastewater are reduced, so that the wastewater reaches a discharge standard. The biological flocculant disclosed by the invention is loaded on cellulose modified montmorillonite, so that the pressure resistance of white-rot fungi to a growth environment is improved, high-concentration wastewater is prevented from inhibiting mycelial growth, and the wastewater treatment efficiency is improved.
Owner:广西桦源环保科技有限公司

Biomass garbage pretreatment method and application

The invention discloses a method for pretreating biomass garbage and application. The method for pretreating the biomass garbage comprises the following steps: inoculating white-rot fungi into slurry containing the biomass garbage, and culturing the white-rot fungi. According to the method for pretreating the biomass garbage, the white-rot fungi are inoculated to the slurry of the biomass garbage for cultivation, so that unwanted microorganisms in the biomass garbage are killed, the biodegradability of the kitchen garbage is improved, traditional chemical and physical and chemical pretreatment is replaced, and therefore the energy cost is reduced.
Owner:SUZHOU XUNIVERSAL BIOTECHNOLOGY CO LTD

Fertilizer for plant growth and preparation method thereof

The invention relates to the technical field of fertilizers, in particular to a fertilizer for plant growth and a preparation method thereof, and the fertilizer is prepared from the following components in parts by weight: 20-60 parts of tylosin mushroom dregs; 10 to 40 parts of straw; 5 to 30 parts of livestock and poultry manure; 1 to 20 parts of calcium superphosphate; 1-10 parts of potassium sulfate; and 1-20 parts of modified humic acid. According to the prepared plant growth fertilizer and the preparation method thereof, the activity and water solubility of the fertilizer are improved by means of modifying humic acid, culturing white-rot fungi, adding carbon nanotubes and montmorillonite and the like, and the adsorption and desorption capability of the fertilizer on soil nutrients is enhanced; meanwhile, the raw materials such as the tylosin residues, the straw and the livestock and poultry manure are used for promoting the flourishing of soil microbial communities and continuously improving the soil fertility, so that the problems of insufficient nutrient content, slow release, low pertinence and the like of organic fertilizers and biological fertilizers in related technologies are solved.
Owner:NINGXIA TAIYICIN BIOTECH CO LTD

Thermophilic ammoniation compound microbial agent, fertilizer and application

The invention belongs to the field of resource utilization of livestock and poultry manure, and particularly relates to a thermophilic ammoniation compound microbial agent, a fertilizer and application. The thermophilic ammoniation compound microbial agent is prepared by compounding lysinibacillus spp., bacillus megatherium and white-rot fungi, the preservation number of the lysinibacillus sphaeroides is CCTCC NO: M 20242776, the preservation number of the bacillus megaterium is CCTCC NO: M 20242777, the preservation number of the white-rot fungi is CCTCC NO: M 20242778, and the three strains are all preserved in the China Center for Type Culture Collection. Tests prove that the thermophilic ammoniation compound microbial agent shows higher ammoniation activity at 60 DEG C, and can be used for promoting the compost recycling process and improving the compost product quality.
Owner:NANJING AGRICULTURAL UNIVERSITY +1

White-rot fungus, fermentation liquor, ammoniating fungus fungicide and application

The invention belongs to the field of resource utilization of livestock and poultry manure, and particularly relates to white-rot fungi, fermentation liquor, a microbial agent and application. The white-rot fungus is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20242778. Tests prove that the ammoniating fungus inoculant prepared from the white-rot fungus shows high ammoniating activity at 30 DEG C, and the ammoniating fungus inoculant can be inoculated to promote the compost recycling process and improve the compost product quality.
Owner:BEIFANG UNIV OF NATITIES +1

Environment-friendly waterproof adhesive for furniture wood board and preparation method of environment-friendly waterproof adhesive

The invention relates to an environment-friendly waterproof adhesive for furniture wood boards and a preparation method thereof, and belongs to the technical field of adhesives. The preparation method comprises the following steps: fermenting and degrading lignin, then adding a phenolic compound, stirring under an alkaline condition, finally adding formaldehyde, heating, preserving heat, and cooling to obtain the environment-friendly waterproof adhesive for the furniture wood board. White rot fungi and bacillus subtilis can effectively degrade lignin during fermentation, ether bonds in lignin are broken, steric hindrance of a molecular structure is reduced, more active sites are formed, meanwhile, phenolic compounds are added, the content of phenolic hydroxyl groups is increased, the active sites are further increased, and the content of lignin is increased. And after being combined with formaldehyde, the environment-friendly adhesive with high adhesion and good waterproofness is obtained.
Owner:DONGGUAN HONGXIN WOOD PROD CO LTD

Fermentation medium for improving enzyme activity of lignin degrading enzyme produced by white-rot fungi and application of fermentation medium

The invention relates to the technical field of microorganisms, in particular to a fermentation medium for improving enzyme activity of lignin degrading enzyme produced by white-rot fungi and application thereof.The fermentation medium comprises a culture container and a controller, the culture container is provided with a driving part, and the driving part is used for driving the culture container to rotate; the controller is used for dividing a fermentation period based on the fermentation time, and the fermentation period comprises a hypha expansion period, an environment coordination period and a fermentation stable period; in the hypha expansion period, the controller controls the driving piece to enable the rotating speed of the culture container to be 400-800 r / min, so that the hypha is cut off by shearing force generated by rotation, and a new growing point is formed; in the environment coordination period, the controller controls the driving piece to enable the rotating speed of the culture container to be 300-400 r / min; and the fermentation stable phase controller controls the driving piece to enable the rotating speed of the culture container to be 100-200r / min. With the adoption of the technical scheme, the rotating speed of the fermentation culture medium is dynamically controlled, so that the effects of dispersing hyphae, cutting the hyphae and damaging the mycelia generated by shearing force of rotation can be regulated in a targeted manner in each hypha growth stage, and the improvement of enzyme activity is promoted.
Owner:GUANGXI UNIV

A root cutting seedling raising method for Lonicera japonica

The present application provides a method for raising seedlings of honeysuckle by root cuttings. The method comprises the following steps: (1) selecting healthy, pest-free, and 0.3 cm thick root strips from a mother tree as cuttings, cutting the root strips into 5-8 cm long strips and disinfecting them; (2) after disinfection, quickly dipping the base of the cuttings with a plant rooting agent, and then soaking the cuttings in a composite nutrient solution comprising the following raw materials: loofah extract, Ailanthone, Acanthopanax senticosus polysaccharide, and ethanol; taking the cuttings out after soaking for 10-15 minutes before cutting; (3) spraying the cuttings alternately with a composite agent and a fungicide every 5-7 days after cutting, at night when the leaf surface is free of water, wherein the composite agent is prepared by extracting gentian, akebia, and kudzu root. The method of the present invention effectively increases the growth rate of honeysuckle seedlings, improves disease resistance to diseases such as brown spot disease, white rot, powdery mildew, etc., promotes healthy and rapid growth, and improves seedling survival rate and seedling quality.
Owner:HUNAN NONGFUGE AGRI DEV CO LTD

White rot fungi WH-B-X2 used as soil biological modifier, application of white rot fungi WH-B-X2, soil biological modifier and preparation method of soil biological modifier

The invention provides white rot fungi WH-B-X2 used as a soil biological modifier, application of the white rot fungi WH-B-X2, the soil biological modifier and a preparation method of the soil biological modifier, and belongs to the technical field of biology. The white rot fungus WH-B-X2 is preserved in China Center for Type Culture Collection on May 8, 2024, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 2024883. The soil biological improver provided by the embodiment of the invention has a good inhibition effect on effective cadmium in soil, can remarkably reduce the cadmium content in crops, and meanwhile, can improve the pH value of the soil, so that the pH value of the soil can be suitable for growth and development of the crops, and the soil is prevented from being too acidic.
Owner:XICHUAN WUHUA BIOTECHNOLOGY CO LTD

A fixed white rot fungus bioreactor and a method for treating pharmaceutical wastewater using the same

The present invention discloses a fixed white rot fungus bioreactor and a method for treating pharmaceutical wastewater using the same, and belongs to the technical field of pharmaceutical wastewater treatment. The fixed white rot fungus bioreactor provided by the present invention is provided with a packing layer, a filter material layer, an aeration layer and a drainage layer from top to bottom, a water distribution pipe is provided on the top of the packing layer, and a water outlet pipe is provided on the side wall of the drainage layer; the packing layer is used to carry white rot fungi, and the packing of the packing layer is a granular packing, which is obtained by mixing sawdust, copper and iron filings and adhesives, pressing into granules, and drying; the drainage layer is composed of a drainage ditch cast by concrete, and the drainage ditch is connected to the water outlet pipe. Through the joint action of the packing layer, the filter material layer, the aeration layer and the drainage layer, it can ensure long-term stable operation, high pharmaceutical wastewater treatment efficiency and good wastewater treatment effect, without the need for frequent packing replacement.
Owner:SHANDONG PACIFIC ENVIRONMENTAL PROTECTION