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52 results about "White rot" patented technology

Method for enhancing degradation of biodegradable plastic compost based on white-rot fungi and manganese modified charcoal

The invention relates to the technical field of organic solid waste resourceful treatment, in particular to a method for enhancing degradation of biodegradable plastic compost based on white-rot fungi and manganese modified charcoal. According to the method, manganese modified charcoal and white-rot fungi are subjected to synergistic treatment, and the efficient method for intensively degrading the biodegradable plastic through aerobic composting is constructed. The method effectively overcomes the technical bottlenecks of slow degradation starting, long degradation period and easy generation of micro-plastic residues of the biodegradable plastic in traditional compost, and realizes rapid and thorough degradation of the biodegradable plastic. Besides, the method also promotes the conversion of humus precursor substances in the compost material and accelerates and stabilizes the synthesis of humus, so that the decomposition degree of the compost product is remarkably improved, and the quality and environmental safety of the final compost product are synchronously improved while the biodegradable plastic is efficiently degraded. And an efficient and reliable solution is provided for recycling of organic solid wastes and degradation of biodegradable plastic compost.
Owner:EAST CHINA NORMAL UNIV

Microbial combined bacteria and method for improving fertility of newly-cultivated land

PendingCN121086908AAgriculture tools and machinesFungiTrichoderma spiraleBacilli
The invention discloses microbial combined bacteria and a method for improving fertility of newly-cultivated land. The method comprises the following steps: combining every two of bacillus amyloliquefaciens WK1, white rot fungi NDM3-2 and trichoderma helichum HJR7 to form three combined bacteria; the three combined bacteria are applied to soil containing straw and lentinus edodes residues respectively, and a pot experiment (rice and white clover crop rotation) for two years is carried out with no addition of any bacteria as a contrast; analyzing the physical and chemical properties of the soil and the microbiological indexes of the soil; and according to an analysis result, the combination of the white rot fungi NDM3-2 and the trichoderma helichum HJR7 is confirmed to be an ideal scheme for improving the fertility of the newly-cultivated land.
Owner:ZHEJIANG FORESTRY UNIVERSITY

Dye degradation system and method using rice straw as carbon source

The application discloses a dye degradation system and method with rice straw as a carbon source, and belongs to the technical field of wastewater treatment. The application successfully constructs an economical combined degradation system by combining the ability of white rot fungi to decompose macromolecular organic matter and the characteristics of the power generation microorganism MR-1 to degrade pollutants in a broad spectrum and efficiently. The system uses rice straw as a carbon source, uses white rot fungi to degrade the rice straw, obtains a degraded straw solution, and then uses the power generation microorganism MR-1 to perform anaerobic respiratory metabolism on the degraded straw solution, releases electrons, and transmits the electrons to dye pollutants, so as to degrade dye wastewater. The combined degradation system can not only make up for the deficiency of the power generation microorganism MR-1 in utilizing macromolecular organic matter carbon sources, but also can significantly reduce the cost of carbon sources, and simultaneously realize the dual goals of wastewater treatment and solid waste resource utilization. The method is environmentally friendly, treats waste with waste, and has potential to be popularized and applied in practical application.
Owner:JIANGSU UNIV

Method for promoting corn straw compost heat production by using exogenous microbial agent

The invention relates to the technical field of microbial agents, in particular to a method for promoting corn straw composting heat production by utilizing an exogenous microbial agent, which comprises the following steps: crushing corn straws, and mixing with chicken manure to obtain a fermentation raw material; laying a first layer of fermentation raw materials on the waterproof bottom film in the fermentation pit, uniformly scattering mixed freeze-dried powder of bacillus cereus, bacillus smith, bacillus thermophilic denitrification soil bacillus, bacillus licheniformis and ureibacillus thermophilus, laying a second layer of fermentation raw materials, and uniformly scattering EM (effective microorganisms) freeze-dried powder; after a third layer of fermentation raw materials is laid, white rot fungi, chaetomium, trichoderma harzianum and aspergillus niger mixed freeze-dried powder is evenly scattered, and after each layer of fermentation raw materials is laid, water is sprayed to adjust the water content; a heat exchange pipeline is laid between the two layers of fermentation materials, air is inflated into the pipeline through an air blower in the fermentation process, the oxygen content is adjusted to be 5%-15%, and high heat yield and efficient degradation of lignin and cellulose are achieved through functional flora construction.
Owner:JILIN UNIVERSITY

A gene related to resistance to white rot of Dendrobium officinale and its application

The present invention discloses a gene related to resistance to white rot of Dendrobium officinale and its application, wherein the CDS sequence of gene Dof013141 is shown in SEQ ID NO.1. The present invention isolates and screens the Dof013141 gene. The overexpression of this gene in Dendrobium officinale successfully overcomes the environmental pollution and drug resistance problems caused by traditional chemical control methods, not only effectively reduces the dependence on chemical agents, but also provides a new technical solution for green and sustainable agricultural prevention and control. The application prospect of this gene is broad. In addition to its successful performance in Dendrobium officinale, it can also be extended to the disease-resistant breeding of other susceptible plants, further broadening its application in the field of agricultural production and plant protection. The present invention not only provides a strong guarantee for improving the yield and quality of Dendrobium officinale, but also opens up a new research direction for the development and utilization of plant disease-resistant genes, with great economic and social value.
Owner:NANJING NORMAL UNIVERSITY

Biomass garbage pretreatment method and application

The invention discloses a method for pretreating biomass garbage and application. The method for pretreating the biomass garbage comprises the following steps: inoculating white-rot fungi into slurry containing the biomass garbage, and culturing the white-rot fungi. According to the method for pretreating the biomass garbage, the white-rot fungi are inoculated to the slurry of the biomass garbage for cultivation, so that unwanted microorganisms in the biomass garbage are killed, the biodegradability of the kitchen garbage is improved, traditional chemical and physical and chemical pretreatment is replaced, and therefore the energy cost is reduced.
Owner:SUZHOU XUNIVERSAL BIOTECHNOLOGY CO LTD

Thermophilic ammoniation compound microbial agent, fertilizer and application

The invention belongs to the field of resource utilization of livestock and poultry manure, and particularly relates to a thermophilic ammoniation compound microbial agent, a fertilizer and application. The thermophilic ammoniation compound microbial agent is prepared by compounding lysinibacillus spp., bacillus megatherium and white-rot fungi, the preservation number of the lysinibacillus sphaeroides is CCTCC NO: M 20242776, the preservation number of the bacillus megaterium is CCTCC NO: M 20242777, the preservation number of the white-rot fungi is CCTCC NO: M 20242778, and the three strains are all preserved in the China Center for Type Culture Collection. Tests prove that the thermophilic ammoniation compound microbial agent shows higher ammoniation activity at 60 DEG C, and can be used for promoting the compost recycling process and improving the compost product quality.
Owner:NANJING AGRICULTURAL UNIVERSITY +1

White-rot fungus, fermentation liquor, ammoniating fungus fungicide and application

The invention belongs to the field of resource utilization of livestock and poultry manure, and particularly relates to white-rot fungi, fermentation liquor, a microbial agent and application. The white-rot fungus is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20242778. Tests prove that the ammoniating fungus inoculant prepared from the white-rot fungus shows high ammoniating activity at 30 DEG C, and the ammoniating fungus inoculant can be inoculated to promote the compost recycling process and improve the compost product quality.
Owner:BEIFANG UNIV OF NATITIES +1

Method for promoting white-rot fungi to degrade plastics through pretreatment technology

The invention relates to the technical field of solid waste treatment, and discloses a method for promoting white-rot fungi to degrade plastics by a pretreatment technology, which comprises the following steps: adding plastics into dimethylbenzene, heating and stirring until the plastics are completely dissolved, uniformly spreading on a glass plate, cooling, separating, air-drying to obtain a plastic film, and shearing the plastic film into a size of 30mm * 30mm to obtain an original polystyrene plastic film; an original polystyrene plastic film is put into a beaker for pretreatment, white-rot fungi degradation is performed after pretreatment, and the plastic degradation effect of the white-rot fungi is enhanced; the problems that in the prior art, plastic molecular chains cannot be efficiently decomposed, the degradation efficiency is low, the energy consumption is high, secondary pollutants can be generated in the degradation process, and engineering application is difficult to achieve are solved.
Owner:EAST CHINA NORMAL UNIV

Berkhoutia calomelanos and application thereof in prevention and treatment of grape diseases

The application discloses a Burkholderia gladioli and application thereof in prevention and treatment of grape diseases, the classification and naming of the bacteria is Burkholderia gladioli, which is preserved in the China General Microbiological Culture Collection Center on April 1, 2024, and the preservation number is CGMCC No.30228, and the address of the preservation unit is No.3, Xili, Beichen West Road, Chaoyang District, Beijing City. The strain has a wide antibacterial spectrum, and has good antibacterial activity on common pathogenic bacteria in grape planting, especially on grape white rot and grape anthracnose. Through directional screening of grape rhizosphere soil microorganisms, a biocontrol bacterium with high efficiency of antagonizing grape white rot bacteria is obtained, and basic materials are provided for biological control of grape white rot.
Owner:SHANDONG ACAD OF GRAPE

Phenolic acid degradation fertilizer and preparation method thereof

The invention provides a phenolic acid degradation fertilizer and a preparation method thereof, raw materials comprise metal cross-linked gel and engineering bacterium spores coated with the metal cross-linked gel, the engineering bacterium spores are one or more of white-rot fungus spores, trichoderma harzianum spores and aspergillus aculeatus spores, and the metal cross-linked gel comprises ferric chloride, sodium alginate and gelatin. Iron chloride, sodium alginate and gelatin form metal cross-linked gel to coat the engineering bacterium spores, phenolic acid pollutants in the environment can chelate metal ions in the metal cross-linked gel, and the engineering bacterium spores in the phenolic acid degradation fertilizer can be released only when the concentration of phenolic acid in the environment reaches a certain threshold value. Further, phenolic acid in the environment is decomposed, and it is ensured that all activity of functional bacteria is delivered to a phenolic acid enriched rhizosphere pollution area.
Owner:HEBEI UNIV OF ENG

Grape endophytic fungus NZ1-b-1 and application thereof in prevention and treatment of grape diseases

The invention belongs to the technical field of microorganisms, and particularly relates to grape endophytic fungi NZ1-b-1 and application thereof in prevention and treatment of grape diseases. The grape endophytic fungus NZ1-b-1 is classified and named as subclavian endophytic fungus and is preserved in the China Center for Type Culture Collection on July 14, 2025, and the preservation number of the grape endophytic fungus NZ1-b-1 is CCTCC (China Center for Type Culture Collection) NO: M 20251604. The grape endophytic fungi NZ1-b-1 provided by the invention are endophytic fungi separated from grape leaves, the culture method is simple, the cost is low, and the separation method is easy to operate. The grape endophytic fungus NZ1-b-1 has an antibacterial effect on grape botrytis cinerea and white rot fungi, has a relatively high prevention and treatment effect on grape gray mold and white rot, has a certain prevention and treatment effect on grape downy mildew, and has the potential of being developed into a broad-spectrum antibacterial agent for grape fungal diseases.
Owner:NORTHWEST A & F UNIV

A single-nitrogen-source regulation medium for white rot fungi, and a preparation method and application thereof

PendingCN122326385ABiotechnologyPhosphate
This invention relates to the field of microbial technology. It provides a culture medium for regulating a single nitrogen source in white-rot fungi, its preparation method, and its applications. The culture medium uses water as a solution and further includes potassium dihydrogen phosphate, disodium hydrogen phosphate, a carbon source, magnesium sulfate heptahydrate, manganese sulfate tetrahydrate, zinc sulfate heptahydrate, copper sulfate pentahydrate, calcium chloride heptahydrate, ferrous sulfate heptahydrate, and a nitrogen source. The core advantage of the culture medium and preparation method designed in this invention lies in the precise control of the type of nitrogen source, effectively eliminating interference from multiple nitrogen sources. This provides a dedicated culture medium for studying the physiological metabolism, molecular mechanisms, and biosynthesis of white-rot fungi under specific nitrogen source environments, and is widely applicable to transcriptional, protein and metabolic regulation, as well as biodegradation and synthetic biology in white-rot fungi.
Owner:NINGXIA ACAD OF AGRI & FORESTRY SCI INST OF ANIMAL SCI (NINGXIA GRASS LIVESTOCK ENG TECH RES CENT)

White rot fungus amplification culture device and method

The invention provides a white rot fungus amplification culture device and method, the device comprises a sterile room provided with a door, the device also comprises a sterilization system, the sterilization system comprises an ozone generator, the ozone generator is communicated with the interior of the sterile room through a pipeline, and the top of the sterile room is also provided with an ultraviolet lamp; the microorganism culture system comprises a sealed culture tank, an air blower communicated with the culture tank is arranged outside the culture tank, a sulfuric acid sterilization tank is communicated with an air blowing pipe between the air blower and the culture tank, the concentration of sulfuric acid in the sulfuric acid sterilization tank is greater than 20%, and the air blowing pipe is communicated with an aeration pipe arranged at the bottom of the culture tank; the top of the culture tank is provided with a bacterial liquid adding bottle, a nutrient solution adding bottle and a one-way exhaust valve, the culture tank is internally provided with filler and a heating ring, the top of the culture tank is provided with a sterile water inlet pipe, and the bottom of the culture tank is provided with a waste liquid discharge pipe. The production efficiency of the white rot fungi can be remarkably improved, and the white rot fungi are easily kneaded and stripped from the filler after being taken out.
Owner:CHINA THREE GORGES CORPORATION +1

Schizophyllum commune DKK and application thereof in prevention and treatment of rice and wheat diseases

The invention discloses a strain of schizophyllum commune DKK and application thereof in prevention and treatment of rice and wheat diseases, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation date is May 19, 2025, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.42010. The DKK strain is separated from persimmon fruits on which sporocarp grows. Researches show that the strain DKK inhibits the growth of rhizoctonia solani through antagonism and hyperparasitism; and the compound also has relatively good antagonistic activity on other pathogenic bacteria such as cochliobotrys miyabeanus, fusarium graminearum, wheat stem rot pathogen, potato late blight pathogen, tomato fusarium wilt pathogen, sclerotium fistulosum, strawberry botrytis cinerea, colletotrichum gloeosporioides and ligustrum japonicum leaf spot pathogen.
Owner:YANGZHOU UNIV

Sulfur-doped mycelium bamboo composite material and preparation method thereof

ActiveCN122380549BBiotechnologyWhite rot
The application belongs to the technical field of composite materials, and specifically provides a sulfur-doped mycelium bamboo-based composite material and a preparation method thereof. The application prepares a potato glucose agar culture medium first, then carries out the propagation of white rot fungi based on the potato glucose agar culture medium, then cultures the bamboo powder carbon powder prepared under specific conditions and the white rot fungi in a liquid culture medium to obtain white rot fungi bamboo powder spheres, finally carries out a dipping reaction of the obtained white rot fungi bamboo powder spheres and sodium thiosulfate, collects the solid material after the reaction is completed, and dries the solid material, finally carries out a heating treatment under a nitrogen atmosphere, and finally the sulfur-doped mycelium bamboo-based composite material is prepared, which has high adsorption of heavy metal ions and can effectively remove heavy metal ions when used for treating mercury-containing wastewater.
Owner:SHANDONG UNIV OF TECH

Biologically inoculated sorptive bead media

A biologically active sorptive media is disclosed for sustainable contaminant removal in, for example, stormwater management systems. This technology addresses the technical problem of hydrophilic trace organic contaminants and dissolved-phase nutrients passing through conventional stormwater infrastructure, posing risks to groundwater and surface water quality. The biologically active sorptive media comprises a biopolymer matrix formed from alginate hydrogel, encapsulating sorbent materials (e.g., powdered activated carbon (PAC), iron-based water treatment residuals (FeWTR]), growth substrates (e.g., wood flour), and biodegrading organisms (e.g., white rot fungi). These beads enable rapid sorption of contaminants during storm events and subsequent biodegradation during inter-storm periods, renewing sorption capacity. The beads are mechanically robust, scalable, and stable for extended periods in high ionic strength solutions. Applications include bioaugmentation of green stormwater infrastructure (GSI), wastewater treatment, and bioremediation. This approach transforms GSI science by coupling sorption and biodegradation for enhanced contaminant removal.
Owner:THE UNIVERSITY OF IOWA RESEARCH

A solid-state fermentation inoculant, its preparation method and application

PendingCN122081081AFungiContaminated soil reclamationBiotechnologyPhanerochaete
This invention provides a solid-state fermentation inoculant, its preparation method, and its application. The preparation method of the solid-state fermentation inoculant involves inoculating *Phanerochaete chrysosporium* into a seed carrier and culturing it in a sterile, pure oxygen environment. Under pure oxygen conditions, the inoculated *Phanerochaete chrysosporium* exhibits a significantly increased reproduction rate, forming a high-density microbial community. Simultaneously, the continuous and stable nutrient supply from the seed carrier enables the inoculant to quickly establish a competitive advantage upon entering materials containing other microorganisms. By seizing nutrients and oxygen resources, it inhibits the growth and reproduction of other microorganisms, ultimately increasing the abundance of white-rot fungi in the fermented product, thereby enhancing the degradation efficiency of petroleum hydrocarbons in the soil. Compared to traditional solid-state fermentation methods, the inoculant production process is very simple and low-cost. The solid-state fermentation inoculant product of this invention has a high microbial density and a relatively large number of target strains, resulting in higher efficiency in soil pollution remediation and increased soil fertility.
Owner:YANGTZE UNIVERSITY

Nicotine-resistant irpex laceratus IL01 and application thereof

The invention discloses nicotine-resistant irpex laceratus IL01 and application thereof, and belongs to the technical field of agricultural microorganisms. The invention provides a tobacco source strain with the fermentation characteristics of nicotine resistance, salt resistance, acid resistance, high yield of lignocellulase activity, aroma production and the like, the tobacco source strain is identified as white-rot fungus irpex laceratus, on one hand, different substrates can be fermented by the strain to prepare tobacco flavors, and the sensory quality of cigarettes can be improved after the cigarettes are perfumed; on the other hand, tobacco source lignocellulose such as stem paste, cut stems, tobacco leaves, tobacco powder, tobacco soil sticks or tobacco straw can be utilized in a high-valued mode and converted into flavor substances. According to the method, a new technical approach can be provided for improving the quality of cigarette products and reducing the cost, a new thought is provided for tobacco biomass resource utilization in the future, the method conforms to the double-carbon development concept, and the method has important significance for promoting agricultural green and low-carbon development and comprehensively achieving rural revitalization.
Owner:TOBACCO RESEARCH INSTITUTE OF CHINESE ACADEMY OF AGRICULTURAL SCIENCES (QINGZHOU TOBACCO RESEARCH INSTITUTE OF CHINA NATIONAL TOBACCO COMPANY)

Microbial agent for preventing and treating common pepper diseases as well as preparation method and application of microbial agent

ActiveCN121176477ABiocidePlant growth regulatorsBiotechnologyKlebsiella variicola
The invention discloses a microbial agent for preventing and treating common pepper diseases and a preparation method and application thereof, and belongs to the technical field of microbial prevention and treatment. The microbial agent is prepared from the following raw materials in parts by weight: 70 to 75 parts of compound microbial liquid, 0.05 to 0.08 part of brown sugar, 0.8 to 1.2 parts of trehalose, 0.2 to 0.3 part of a surfactant, 1.8 to 2.5 parts of potassium sorbate and the balance of deionized water, wherein the total parts by weight of the raw materials are 100; the compound microorganism bacterial liquid is prepared from klebsiella variicola, marine bacillus acksu and bacillus subtilis. According to the invention, Klebsiella variicola, Marinobacter acksu and bacillus subtilis are screened to form a compound microorganism bacterium solution, the three bacterium solutions are mixed according to a specific ratio to synergistically inhibit the growth of pathogenic bacteria, and the compound microorganism bacterium solution has a good antagonistic effect on pepper fusarium wilt, southern blight and phytophthora blight, and has an optimal inhibitory effect on pepper phytophthora blight. The microbial agent provided by the invention significantly improves the fruit number of a single capsicum plant and the health degree of the capsicum plant, has safe and environment-friendly components, is non-toxic to capsicum, and effectively prolongs the storage period of capsicum.
Owner:HUBEI MAOSHENG BIOLOGY CO LTD

Preparation method and application of high-cohesiveness environment-friendly antibacterial woodworking glue

The invention discloses a preparation method and application of high-cohesiveness environment-friendly antibacterial woodworking glue. The woodworking glue is a water-based emulsion, and is prepared through the processes of prepolymerization of polyisocyanate and polyol, chain extension of a hydrophilic monomer, a chain extender and a silane coupling agent, end capping and neutralization of an imidazole compound, emulsification and post-chain extension. The adhesive developed by the invention takes water as a dispersion medium, does not contain formaldehyde, is low in VOC (Volatile Organic Compounds), and is safe and environment-friendly. A molecular side chain of the adhesive contains hydrolyzable siloxane, a terminal group contains thermal reactive blocked isocyanate, and when the adhesive is applied, self-crosslinking of adhesive molecules can be realized, and covalent bonds can be formed with hydroxyl groups on the surface of wood, so that the adhesive has high cohesive force and strong interface adhesion. A hydrophobic structure formed by siloxane chain segments can further improve the hydrolysis resistance of the adhesive. Besides, the glue system has a cationic characteristic, and the imidazole compound released by curing and the side chain tertiary amine salt have a synergistic effect, so that breeding of microorganisms such as white-rot fungi and the like can be efficiently inhibited. The adhesive is suitable for bonding wood materials, can be heated and cured after being glued and attached, and has good application value in manufacturing of various wood products.
Owner:QILU UNIVERSITY OF TECHNOLOGY (SHANDONG ACADEMY OF SCIENCES)

Composite bottom mud repairing agent as well as preparation method and application thereof

The invention belongs to the technical field of bottom mud repair, and particularly relates to a composite bottom mud repair agent as well as a preparation method and application thereof. The composite bottom mud repairing agent comprises a main functional component, an auxiliary functional component and a stabilizer, the main functional components comprise modified attapulgite, a compound microbial agent and nano-hydroxyapatite; the auxiliary functional components comprise straw carbon, an adhesive and humate; the modified attapulgite is obtained by modifying attapulgite with an acid solution and a surfactant; the compound microbial agent comprises pseudomonas, bacillus, white-rot fungi, compound nitrifying bacteria and phosphorus-accumulating bacteria. Through the modified attapulgite, the compound microbial agent and the nano-hydroxyapatite, and in combination with other components, heavy metals, organic pollutants and organic nitrogen and phosphorus in the polluted bottom mud can be efficiently removed; the microenvironment and structure of the bottom mud can be improved, and the environmental adaptability is improved.
Owner:GUANGZHOU ZHONGHE ENVIRONMENTAL TECH CO LTD

Enzymatic biological degumming method of industrial hemp fiber

PendingCN121575490AFungiMicroorganism based processesCelluloseVeratryl alcohol
The invention discloses an enzymatic biological degumming method of industrial hemp fiber, and relates to the field of degumming of industrial hemp fiber, according to the enzymatic biological degumming method of industrial hemp fiber, MnP enzyme is produced by white rot fungi, veratryl alcohol is added as a mediator, and the MnP enzyme and the veratryl alcohol form an oxidation-reduction cycle; and colloid components such as lignin-hemicellulose compounds in the raw industrial hemp can be efficiently degraded. Compared with the prior art, the residual gum rate is greatly reduced to 1.2%-1.8% and is reduced by 60%-85% compared with a contrast, the degumming rate is larger than or equal to 98%, and the degumming effect is quite remarkable. Meanwhile, ultrasonic pretreatment destroys a wax coat on the surface of the fiber and promotes enzyme liquid permeation, and mild biological catalysis conditions are combined, so that damage to the fiber is reduced to the maximum extent. Due to the series of advantages, the degummed industrial hemp fibers are excellent in key indexes such as strength and whiteness, and completely meet the quality requirements of high-end textile products.
Owner:QIQIHAR UNIVERSITY

Microbial complex microbial inoculant for repairing petroleum hydrocarbon polluted soil and repairing method and application of microbial complex microbial inoculant

The invention relates to the technical field of microbial remediation, in particular to a microbial complex microbial inoculant for remediation of petroleum hydrocarbon contaminated soil and a remediation method and application of the microbial complex microbial inoculant. The complex microbial inoculant is composed of sulfate reducing bacteria, methanogens, oleophilic bacteria and white-rot fungi. The ratio of the sulfate reducing bacteria to the methanogens to the oleophilic bacteria to the white-rot fungi is (2-4): (0.5-2): (3-5): (1-3) according to inoculation biomass. The remediation method comprises the following steps: inoculating bacterial liquid of sulfate reducing bacteria and methanogens to petroleum hydrocarbon contaminated soil below a certain depth, and continuously remediating for 2-5 weeks; the surface layer of the contaminated soil is inoculated with bacterial liquid of oleophilic bacteria and white-rot fungi, and continuous remediation is conducted for 2-5 weeks. According to the method, a strategy of anaerobic-aerobic two stages and fungus-bacteria transboundary synergy is utilized, refined remediation of a polluted site is realized, and finally, the complex and stubborn petroleum hydrocarbon pollutants are fully degraded by utilizing a mutual synergy effect after multiple microorganisms are compounded.
Owner:SHAANXI INST OF BIOLOGICAL AGRI

Complex microbial inoculant for rapid decomposition of garden waste as well as preparation method and application of complex microbial inoculant

The invention relates to the technical field of microorganism application, in particular to a complex microbial inoculant for rapid decomposition of garden waste and a preparation method and application of the complex microbial inoculant. The microbial strain is prepared by mixing and fermenting white-rot fungus fermentation liquor, cellulose decomposition bacteria fermentation liquor and bacillus fermentation liquor according to the volume ratio of (30-40%): (20-30%): (30-50%); the bacteria content of the white-rot fungus fermentation liquor is 1 * 10 <-1 > * 10 < cfu > / mL, the bacteria content of the cellulose decomposition bacteria fermentation liquor is 1 * 10 <-1 > * 10 < cfu > / mL, and the bacteria content of the bacillus fermentation liquor is 1 * 10 <-1 > * 10 < cfu > / mL. The problem that traditional composting consumes too long time is solved, the treatment efficiency is improved, the strain ratio and process parameters are optimized, the decomposition capacity on lignin, cellulose and other refractory components is enhanced, and efficient conversion of organic matter in waste is promoted.
Owner:TAIZHOU XINYI ENVIRONMENTAL CONSTRUCTION ENGINEERING CO LTD +1

Diversified regeneration method of inactivated SCR denitration catalyst

The invention discloses a diversified regeneration method of an inactivated SCR (Selective Catalytic Reduction) denitration catalyst, which comprises the following steps: firstly, carrying out apparent inspection, performance test and comprehensive analysis on the inactivated catalyst to determine the variety, distribution and degree of toxic substances; next, physical, chemical, thermal regeneration and bioremediation are carried out in this way. Surface and pore channel blockages are removed through physical repairing; chemical remediation parameters are determined according to poisoning types; grading roasting is carried out in thermal regeneration in an inert atmosphere, and parameters are determined according to poisoning degrees; in the bioremediation process, white-rot fungi are used for degrading organic toxicants. A poisoning type identification and evaluation system is established, different poisoning mechanisms are clarified, and a basis is provided for repair. Multiple means are integrated, and targeted, efficient and overall activity recovery is achieved. The method emphasizes grading restoration under precise diagnosis, reduces energy consumption and agent dosage, promotes regeneration to develop towards the environment-friendly, economic and sustainable direction, provides a practical and green scheme for the industry, and assists emission reduction and environmental protection.
Owner:SHANGHAI SHIDONGKOU NO 2 POWER PLANT HUANENG INTERNATIONAL POWER CO LTD +2

Method for degrading plastic film by using white-rot fungi

The invention relates to the technical field of solid waste treatment, and particularly discloses a method for degrading a plastic film by using white-rot fungi, which comprises the following steps: inoculating the white-rot fungi into a solid matrix, then adding the plastic film to be degraded, and carrying out solid state fermentation at 30-37 DEG C for 40-50 days. After solid-state fermentation, white-rot fungi are effectively planted on the surface of the plastic film, and oxidative depolymerization and mineralization of the plastic film are promoted, so that degradation of the plastic film is realized.
Owner:EAST CHINA NORMAL UNIV

Treatment method for improving long-acting mildew-proof and antibacterial performance of wood and bamboo materials at low cost

The invention discloses a low-cost treatment method for improving long-acting mildew-proof and antibacterial properties of wood and bamboo materials, which comprises the following steps: S1, treating white rot fungi to the surface and the interior of the wood and bamboo materials, and then culturing; and S2, a mildew-proof antibacterial agent is treated into the wood and bamboo wood infected by the white rot fungi, and the long-acting mildew-proof modified wood and bamboo wood can be obtained. Compared with traditional enzyme catalysis modification, various enzyme molecules are generated in the bamboo wood, the stability of the enzyme is high, the accessibility to a mildew-proof antibacterial agent and catalytic substances of main components of the bamboo wood is high, the catalytic efficiency is high, and the enzyme catalysis cost is low. The white rot fungi are utilized to secrete multiple enzymes in the bamboo wood degradation process, the multiple enzymes cooperate to form a stable catalytic system, through regulation and control of the whole treatment process, the mechanical property of the wood and bamboo wood can be not reduced or even improved, and after modification treatment is conducted through the antibacterial agent, the hardness and the elastic modulus of the bamboo wood are remarkably improved compared with those of the bamboo wood not treated through the antibacterial agent.
Owner:ZHEJIANG FORESTRY UNIVERSITY +1

Fermentation method of straw and Chinese herbal medicine compounded mutton sheep feed

The invention relates to the technical field of feed processing, and discloses a straw and Chinese herbal medicine compounded mutton sheep feed fermentation method which comprises the following steps: firstly, mixing crop straws and Chinese herbal medicine auxiliary materials, carrying out mechanochemical activation, inoculating a complex microbial inoculant, carrying out aerobic fermentation, and degrading lignin by using white-rot fungi and generating phenoxy free radicals; then, an oxidative coupling initiation solution prepared from manganese sulfate monohydrate, tannic acid and a citric acid buffer solution is sprayed to the materials, free radicals are induced to be subjected to a coupling polymerization reaction, and oxygen in gaps of the materials is rapidly consumed through a chemical path so as to establish an anaerobic environment; and finally sealing and carrying out anaerobic fermentation. The technical problem that physical compaction and oxygen removal of straw materials are not thorough is solved through a chemical oxygen consumption mechanism, the degradation rate of lignocellulose is remarkably increased through the synergistic effect of biological enzymolysis and chemical oxidation, phenolic acid antibacterial components in Chinese herbal medicine are effectively converted, dominant growth of lactic acid bacteria is guaranteed, and the method is suitable for industrial production. And the fermented feed with excellent quality and high digestibility is obtained.
Owner:ZHUMADIAN XIANYUAN ANIMAL HUSBANDRY CO LTD

Use of porous support materials in microbial immobilization

The application discloses application of a porous carrier material in microbial immobilization and belongs to the technical field of microbial immobilization. a The porous carrier material has a positive surface (pK a 8) and moderate hydrophilicity (contact angle of 20-60 degrees), is used for immobilization of microorganisms, especially white rot fungi, and obtains a porous carrier-microorganism composite material, which realizes efficient organic matter degradation and stable activity of the immobilized white rot fungi in actual wastewater without pH adjustment, solves the technical problem that the white rot fungi can only be used under acidic conditions in the prior art, and provides a new idea for application of functional microorganisms and degradation of emerging pollutants.
Owner:NANJING UNIV