Lignin degradation solution and preparation method thereof as well as method for degrading lignin by using lignin degradation solution

Abstract

The invention discloses a lignin degradation solution and a preparation method thereof as well as a method for degrading lignin by using the lignin degradation solution, and belongs to biochemical and bio-refinery methods to solve the problem that the efficient biodegradation of lignin is difficult to realize by adopting single ligninase of laccase at present. The lignin degradation solution is prepared by the following steps: dissolving laccase and manganese peroxidase into an acetic acid-sodium acetate buffer solution of which the pH value is 4-6 in an enzyme load ratio of (10:1)-(1:5) to ensure that the enzyme loads of the laccase and manganese peroxidase are respectively 1U / ml-50U / ml and 1U / ml-50U / ml; and then adding 1-10mM of MnSO4 and 0.1-1mM of H2O2, wherein the laccase and manganese peroxidase are obtained by fermenting white rot fungi to obtain extracellular crude enzyme liquid and then performing separation and purification respectively. According to the lignin degradation solution and the methods disclosed by the invention, the synergistic oxidative degradation of macromolecular lignin with rich structural diversity is realized, and compared with a degradation reaction system with single ligninase, the degradation rate of the macromolecules of the lignin can each 30-50%, the degradation conversion efficiency is significantly improved, and the degradation solution and the methods can be applied to the fields of bio-refinery of lignocelluloses, biological pulping or environmental treatment and the like.

Description

technical field

[0001] The invention belongs to biochemical and biorefining methods, and in particular relates to a lignin degradation liquid, a preparation method and a method for degrading lignin with the same. Background technique

[0002] With the continuous depletion of fossil resources, the use of biorefining technology to obtain liquid fuels such as ethanol and butanol from lignocellulosic raw materials, as well as chemical materials and chemicals will become an important supplement to petroleum refining. Lignin, which is highly cross-linked by a variety of phenylpropane-based structural units, is the main component of lignocellulose and one of the most abundant biopolymers on earth. The biotransformation of lignin is extremely difficult, which not only limits The conversion of polysaccharide components such as cellulose and hemicellulose in lignocellulose is also a major component of biorefinery waste. Therefore, the efficient degradation and conversion of lignin is...

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