10 results about "Lignin degradation" patented technology

This invention belongs to the field of textile engineering technology and relates to a method for improving the performance of regenerated lignocellulose fibers based on lignin degradation. Corn stalks are mixed with an ionic liquid and tyrosine, then heated to dissolve, obtaining a spinning solution. The spinning solution is then subjected to wet spinning, and the spun fibers are solidified in a coagulation bath to obtain regenerated fibers. Compared with existing technologies, this invention uses corn stalks as the spinning raw material, which is widely available and inexpensive. Furthermore, this invention can achieve complete dissolution of the stalks and effectively remove lignin, reducing the lignin content in the regenerated fibers and improving their performance.

This invention relates to the field of genetic engineering technology, and more particularly to an engineered bacterium with high substrate utilization efficiency, its construction method, and its applications. The method includes overexpressing laccase and lignin peroxidase in two monokaryotic Ganoderma lucidum strains, respectively, and then hybridizing the two monokaryotic strains to form a new Ganoderma lucidum. Ganoderma lingzhi MKLGE253252, depositary institution: China General Microbiological Culture Collection Center; address: No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing; deposit date: March 11, 2026; accession number: CGMCC No. 42594. It synergistically enhances lignin degradation capacity, mycelial utilization efficiency, and growth capacity, and significantly improves the strength of mycelial fiber sheath. This invention fundamentally solves the problems of low lignin utilization efficiency, slow mycelial growth, and insufficient mycelial sheath strength in existing technologies, providing a completely new technical path for the production of bio-based leather.

The application discloses a kind of microbial inoculum for improving humification efficiency of biological drying and rotting process and method thereof.The effective component of the microbial inoculum is composed of high-temperature-resistant strains, such as Saccharomonospora viridis, Pseudomonas taiwanensis, Microtus luteus, Bacillus licheniformis and Bacillus subtilis.The microbial inoculum provided by the application can significantly increase bacterial abundance when applied to the biological drying and rotting system of organic waste, accelerate the temperature rise and dehydration of the substrate in the biological drying stage, and make the system enter the high-temperature stage earlier.The lignin degradation rate is increased by more than 20%, the demethoxylation rate of lignin and the breaking rate of β-O-4 bond are increased, and more aromatic skeletons rich in phenolic hydroxyl groups are produced as key humic acid precursors for HA synthesis.The lignin-protein and polyphenol pathways are enhanced, and the seed germination index and humification ratio of compost products are significantly increased by more than 20%.

The application provides a Klebsiella variicola M15C3 and application thereof, and belongs to the technical field of agricultural microorganisms. The application provides the Klebsiella variicola M15C3, and the preservation number of the Klebsiella variicola M15C3 is CGMCC NO.26628. The Klebsiella variicola M15C3 provided by the application has the lignin degradation characteristics, can promote the decomposition of straw returned to the field, and improves the utilization efficiency of straw returned to the field; the Klebsiella variicola M15C3 has the phosphorus solubilizing ability, can improve the content of available phosphorus in soil, and improves the soil environment; the Klebsiella variicola M15C3 can secrete growth hormones, promotes the seed germination of crops, promotes the growth of crops, and realizes the yield increase of crops. The Klebsiella variicola M15C3 provided by the application has a positive effect in accelerating the transformation of straw returned to the field, improving the soil fertility and promoting the growth of crops.

The application discloses a kind of crop straw lignin efficient degradation method based on the expansion pretreatment of fungus alga collaborative ladder degradation, belong to agricultural waste resource utilization technical field.The present application uses crop straw as raw material, in turn through physical pretreatment, compound fungi aerobic deep depolymerization, three stages of depth transformation of fungus alga symbiotic system, and in each stage sectional addition functional adjuvant, realize lignin efficient depolymerization, degradation speed promotion and nutrient loss reduction.The present application solves the technical defects such as insufficient wall breaking, low lignin degradation rate, long fermentation period, large nutrient loss of traditional straw degradation process.Compared with conventional process: lignin total degradation rate is greater than or equal to 55%, total straw degradation rate is greater than or equal to 70%, fermentation period is shortened by greater than or equal to 24 h, and nutrient loss is less than or equal to 30%.The present application process is mild, wide in scope, green and safe, and the product can be used for biological feed, biological organic fertilizer, soil conditioner, biomass resource utilization, and is suitable for large-scale industrialization promotion.

本发明公开了一株土壤短芽孢杆菌及其降解木质素的应用，技术领域为微生物技术领域，微生物分类命名为：土壤短芽孢杆菌(Brevibacillus agri)，菌株名称为 G‑5‑2，保藏号为CGMCC No.36418。本发明菌株G‑5‑2在甘蔗叶与甘蔗滤泥共堆肥高温期样品中筛选得到。所述菌株 G‑5‑2木质素酶活较高，特别适用于甘蔗渣等酚型结构木质素的降解。

This invention discloses a lignin-degrading bacterium, a bacterial agent, and their applications for the degradation of lignin in bamboo. The bacterial agent comprises a mixture of two lignin-degrading bacteria: *Paracremonium sp.* and *Clonostachys compactiuscula*. *Paracremonium sp.* was deposited with the China General Microbiological Culture Collection Center (CGMCC) on November 7, 2022, with accession number CGMCC No. 40343. *Clonostachys compactiuscula*, belonging to the genus *Clonostachys*, was also deposited with the same center on November 7, 2022, with accession number CGMCC No. 40344. This invention yields two strains capable of efficiently degrading lignin, exhibiting excellent degradation effects on biomass raw materials and suitable for the biological degradation of lignin in bamboo. The two lignin-degrading bacteria and their combined bacterial agent provided by this invention can degrade lignin in biomass and demonstrate excellent degradation effects on bamboo.

The application discloses a method for transforming organic waste by using actinomyces XH2 and black soldier fly larvae in cooperation, and belongs to the technical field of organic solid waste resource utilization. The method sequentially comprises the following steps: preparing ingredients by using poultry manure, straw and a trapping agent, adjusting the water content, inoculating actinomyces XH2, adding humic acid in the first turning, adding pyrite powder in the second turning, adding calcium superphosphate powder in the third turning, adding shell powder and zeolite powder to prepare fermentation feed after 15 days of total turning and fermentation, and feeding black soldier fly larvae with the fermentation feed. The actinomyces XH2 has high cellulose and hemicellulose degradation activity and low lignin degradation activity, lignin is retained to increase the loose and breathable property of the pile, the black soldier fly larvae can easily drill and feed, heavy metals are fixed through time setting, and high-quality black soldier fly larvae protein feed and worm manure organic fertilizer can be obtained.

This invention discloses a lignin-degrading microorganism, its isolation method, and its application, relating to environmental microbiology technology and the field of biodegradation. The isolation method includes: enriching the sample to be isolated using alkaline lignin as the sole carbon and energy source; then using morphological recognition-based single-cell sorting technology and resazurite staining to screen for active core strains that grow well in a single lignin culture medium and can dissolve lignin precipitates; and finally, sequencing identification to obtain a pure microbial strain that degrades lignin. This isolation method can effectively isolate lignin-degrading microorganisms, is simple and efficient, and is suitable for large-scale applications. Furthermore, this invention identifies [specific microorganisms] based on this isolation method. Phytobacter ursingii It is an active core strain for degrading lignin, with outstanding lignin degradation efficiency, providing an important strain resource for developing microbial-based biological treatment technology for lignin waste.

This invention belongs to the field of biomass pretreatment technology, specifically relating to a cobalt-magnesium-aluminum hydrotalcite, its preparation method and application, and a method for biomass pretreatment. The cobalt-magnesium-aluminum hydrotalcite provided by this invention has a molar ratio of aluminum, cobalt, and magnesium of 0.6–0.8:2.2–2.4:1, and a specific surface area of ​​280.2–291.4 m². 2 / g, with an average pore size of 1-4 nm. This invention also provides a method for biomass pretreatment, comprising the following steps: mixing biomass, a catalyst, and methanol, and then performing thermocatalytic degradation in an oxidizing atmosphere to obtain lignin degradation products; the catalyst is the cobalt-magnesium-aluminum hydrotalcite described in the above technical solution or the cobalt-magnesium-aluminum hydrotalcite prepared by the preparation method described in the above technical solution. This invention uses cobalt-magnesium-aluminum hydrotalcite with a specific structure as a catalyst to remove and degrade lignin in biomass in an oxidizing atmosphere, which can improve the retention rate of hemicellulose and cellulose.