49 results about "Lignin degradation" patented technology

The invention belongs to the field of biotechnology and environmental governance, and particularly relates to a manganese peroxidase mutant based on high-throughput screening modification and application of the manganese peroxidase mutant to straw degradation. Aiming at the problems of low natural secretion amount, insufficient specific activity, complex preparation process and the like of the existing manganese peroxidase, the method comprises the following steps: firstly, constructing engineering bacteria based on an escherichia coli exocrine system, constructing a mutant library through error-prone PCR in combination with a directed evolution technology, and performing high-throughput screening by utilizing an ABTS colorimetric method; the recombinant manganese peroxidase mutant with high catalytic activity is obtained, and the enzyme activity is improved by 1.29 times compared with that of a wild type. Furthermore, recombinant engineering bacteria are constructed on the basis of a bacillus subtilis exocrine system, and efficient secretion of the mutant enzyme is achieved. The mutant enzyme preparation is used for wheat straw degradation, and the lignin degradation rate reaches 41.37% (improved by 30.09% compared with that of a wild type). The method is simple and convenient in process, low in cost and remarkable in effect, and has a wide industrial application prospect.

The invention discloses a salt-alkali-tolerant growth-promoting ectopseudomonas DHS-HJ-IAA-2 strain and an application thereof, and belongs to the technical field of microorganisms. According to the invention, a strain of salt-resistant, alkali-resistant and drought-resistant pseudomonas sp. DHS-HJ-IAA-2 is screened, and the pseudomonas sp. DHS-HJ-IAA-2 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36164. Experimental results show that the ectopseudomonas DHS-HJ-IAA-2 has the functions of producing IAA, fixing nitrogen, dissolving potassium, degrading cellulose and lignin, inhibiting phytopathogen and the like, and can remarkably improve the bacteria abundance of saline-alkali soil, improve soil fertility and promote plant growth in a saline-alkali environment. The invention provides a new strain resource and a technical means for improving saline-alkali soil and planting crops on the saline-alkali soil, and realizes improvement of the saline-alkali soil from two aspects of soil-crop-microorganism comprehensive action and microorganism-plant synergistic action.

An enzymatic bioremediation reactor device for the accelerated composting of lignocellulose material, consisting of a sealed, insulated container, a mechanical pretreatment unit and an automated laccase enzyme supply system.

This invention belongs to the field of textile engineering technology and relates to a method for improving the performance of regenerated lignocellulose fibers based on lignin degradation. Corn stalks are mixed with an ionic liquid and tyrosine, then heated to dissolve, obtaining a spinning solution. The spinning solution is then subjected to wet spinning, and the spun fibers are solidified in a coagulation bath to obtain regenerated fibers. Compared with existing technologies, this invention uses corn stalks as the spinning raw material, which is widely available and inexpensive. Furthermore, this invention can achieve complete dissolution of the stalks and effectively remove lignin, reducing the lignin content in the regenerated fibers and improving their performance.

A strain Y26 for degrading lignin, characterized in that the strain Y26 is Acinetobacter baumannii (ATCC 19606T) Acinetobacter baumannii ), preserved in the China Center for Type Culture Collection, located at No. 299, Bajiyuan Road, Wuchang District, Wuhan City, Hubei Province, China, with a preservation number of CCTCCM20242944 and a preservation date of December 30, 2024. The Acinetobacter baumannii Y26 in the present application has excellent ability to degrade lignocellulose, with a Lac enzyme activity of 60.38±2.5 U / L, a Mnp enzyme activity of 2612.14±25 U / L, a Lip enzyme activity of 1460.62±14 U / L, and a lignin degradation rate in corn stalks of 38.93%. In the present application, the corn stalks are pretreated by using the Acinetobacter baumannii Y26 in combination with maleic acid, so that the cellulose conversion rate of the corn stalks reaches 87.41%, the glucose yield reaches 0.668 g / g of stalks, and the prepared saccharification liquid has small inhibition effect on microbial fermentation, effectively improving the ethanol yield.

The invention discloses a buffalo rumen source lignin and cellulose degrading bacterium microecological preparation as well as a preparation method and application thereof, and relates to the technical field of microorganisms. The active ingredient of the microecological preparation comprises bacillus sorola desert, and the preservation number of the bacillus sorola desert in the China Center for Type Culture Collection (CCTCC) is CCTCC NO: M2025512. According to the invention, a strain of bacillus sorola, which has lignin degradation ability and can degrade cellulose, is screened from a rumen solid-liquid mixture sample obtained from a buffalo rumen fistula, and is developed into a microecological preparation. Experimental verification shows that the degradation rate of the strain to sodium lignin sulfonate in 24 hours reaches 9.54%, the degradation rate of the strain to straw in 3 days can reach 8.6% or above, and in an artificial simulation rumen test, lignin in the straw can be remarkably degraded, and meanwhile, the fiber utilization value of the straw is effectively improved.

The invention discloses an efficient lignocellulose degradation compound strain and application thereof in producing methane by fermenting straws. The lignocellulose degradation compound strain is prepared from phanerochaete chrysosporium, oyster mushroom, phoenix mushroom and talaromyces pinophilus. When the composite strain is used for pretreating straws, the degradation rate of cellulose in the straws reaches 77.86%, the degradation rate of hemicellulose in the straws reaches 21.01%, and the degradation rate of lignin in the straws reaches 34.85%; when the pretreated straw and the excrement are subjected to anaerobic fermentation together, compared with a control group, the total gas production rate is increased by 86.28%, and the methane production rate is increased by 98.95%. Therefore, the invention provides a green and efficient straw resource utilization technology so as to synchronously realize the purposes of waste reduction, energy recovery and fermentation residue resource utilization.

The invention relates to BiOX (X = halogen elements Br, I and Cl)-loaded PVA hydrogel and a preparation method and application thereof, the BiOX (X = halogen elements Br, I and Cl)-loaded PVA hydrogel is composed of Bi (NO3) 3.5 H2O, KX (X = Br, I and Cl) and a PVA solution, and PVA / BiOX composite hydrogel is formed; pVA / BiOX hydrogel is used as an efficient carrier, effective loading and dispersion of BiOX nanoparticles are realized through hydrogen-rich bonds on the surface, the pore structure of the hydrogel is enhanced through foaming crosslinking, the diffusion rate of lignin molecules is increased, and thus the performance of photocatalytic degradation of lignin is enhanced. The catalyst is low in preparation cost, mild in preparation condition, convenient to operate and convenient for large-scale production in industry. The PVA / BiOX catalyst provided by the invention is applied to the field of photocatalysis, has good photocatalytic degradation performance on lignin, provides an efficient and environment-friendly method for lignin degradation, and has a wide application prospect.

The invention belongs to the technical field of composite strains, particularly relates to a high-lignin straw fermentation composite microbial agent, and a preparation method and a fermentation process thereof, and aims to solve the problems of low lignin degradation efficiency, long period and high cost of a single microorganism in the prior art. According to the invention, a composite synergistic system of coriolus versicolor, bacillus subtilis and pichia pastoris is innovatively adopted, and efficient degradation is realized through a mechanism that the coriolus versicolor secretes a lignin degrading enzyme system, the bacillus subtilis assists cellulose decomposition and the pichia pastoris optimizes a microenvironment; the preparation method of the complex microbial inoculant comprises the following steps: culturing various strains in a proper culture medium to a specific growth state, and mixing according to an optimized volume ratio; the fermentation process relates to high-lignin straw pretreatment, inoculation, sealed fermentation under control conditions and regular ventilation, the lignin degradation rate can be increased, the feed nutritional value can be improved, the relative feeding value and quality can be improved, and economic and environment-friendly technical support is provided for resource utilization of agricultural by-products.

The invention relates to a thermophilic carbon monoxide streptomyces and a complex microbial inoculant, a preparation method and application thereof, and belongs to the technical field of microorganisms, the classification name of the strain is thermophilic carbon monoxide streptomyces, the name of the strain is G-4-7, and the preservation number of the strain is CGMCC No.36417. The efficient lignin degradation complex microbial inoculant can be prepared from the thermophilic carbon monoxide streptomyces, Brevibacillus agri G-5-2 and Chaetomium thermophilum by using the thermophilic carbon monoxide streptomyces, the name of the strain is G-4-7, and the preservation number of the strain is CGMCC No.36417. The complex microbial inoculant can efficiently degrade bagasse lignin in a high-temperature environment, enzyme systems of all strains are complementary, the degradation efficiency is synergistically improved, resource utilization of bagasse rich in lignin can be promoted, and a technical basis is provided for a green treatment mode of agricultural waste.

The invention belongs to the technical field of microorganisms, and particularly relates to a thermophilic strain B-60-8 for straw degradation and decomposition and application of the thermophilic strain B-60-8. The thermophilic strain B-60-8 is classified and named as thermophilic thermophilic strain B-60-8, and is preserved in China General Microbiological Culture Collection Center (CGMCC) on August 19, 2025, and the preservation number is CGMCC No.35676. The invention aims to obtain a single-function strain which can adapt to a high-temperature period of straw composting and efficiently crush lignin by utilizing H2O2 by establishing a straw decay promoting strategy oriented to H2O2 production capacity and verifying the high efficiency of the strain in straw degradation in a high-temperature environment, so that the problems of poor high-temperature adaptability of a microbial agent and low lignin degradation efficiency are solved, and the application prospect is wide. And a new microbial resource is provided for the practical application of straw decomposition and biological fertilizer preparation.

The application discloses a Talaromyces with multi-oxidase synergistic expression capacity and a fermentation method and application thereof. Talaromyces sp. The Talaromyces (Talaromyces autumnalis) Ta-lac is preserved in the China General Microbiological Culture Collection Center on July 11, 2025, and the preservation number is CGMCC No. 42125. Compared with the engineering strain for biosynthesis of laccase, the strain has strong environmental adaptability, can naturally synthesize laccase, and has relatively stable genome, no potential risk of exogenous genes and relatively high safety. The Talaromyces has a short growth cycle, high yield of laccase, nitropropane dioxygenase, hydrogenase coenzyme dioxygenase and squalene monooxygenase and high lignin degradation activity in a fermentation process. The Talaromyces Ta-lac is applied to degradation of toxin dye wastewater, and has remarkable detoxification and decolorization effects.

The invention discloses bacillus tequilensis with lignin degradation and black shank antagonism functions and application of the bacillus tequilensis JZF3, the bacillus tequilensis JZF3 is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation date is June 9, 2025, and the preservation number is CGMCC No.34784. The bacillus tequilensis JZF3 can be used for degrading lignin and antagonizing black shank. The bacillus tequilensis JZF3 has the functions of lignin degradation and shin disease antagonism, and meanwhile, a metabolite of a lignin degradation solution of the bacillus tequilensis JZF3 has the effect of promoting the growth of plants, so that the bacillus tequilensis JZF3 has a relatively high market prospect.

The invention discloses a laccase mutant and application thereof, the mutant is obtained by mutating wild laccase BstCotA from bacillus subtilis, the amino acid sequence of the mutant is as shown in SEQ ID NO.1, and the mutant is mutated into at least one of E186A, N65G, D185G and N311G. The invention further discloses a preparation method of the laccase mutant. Compared with a wild type, the mutant has the advantages that the catalytic activity is obviously improved, and the relative enzyme activity is higher under the conditions that the temperature is 37 DEG C and the pH value is 5.0; the pH stability and the thermal stability are enhanced, and the activity in the range of 40-90 DEG C exceeds 75%; the dye decolorization efficiency is higher, the decolorization rate of amino black 10B reaches 28%-35%, the decolorization rate of crystal violet reaches 35%-45%, and complex printing and dyeing wastewater can be efficiently treated; the lignin degradation capacity is better, the degradation rates within 1 hour and 2 hours are higher than those of the wild type, and pulping bleaching and biomass resource utilization are facilitated.

The invention discloses a resource utilization method of bamboo waste materials, which comprises the following steps: S1, washing the bamboo processing production waste materials until the water content of the bamboo processing production waste materials reaches 50-60%, then carrying out steam explosion treatment at 180-200 DEG C, obtaining steam explosion liquid containing lignin degradation products at a condensate interface, and recycling the steam explosion liquid containing lignin degradation products at the condensate interface; steam explosion solids containing cellulose are obtained in the material collecting cavity; s2, loading the steam explosion solid into an acid and alkali resistant filter bag, transferring into an enzymolysis system, adding a buffer solution into the enzymolysis system, and carrying out an enzymolysis reaction at 45-50 DEG C to obtain an enzymolysis product; and S3, transferring the enzymolysis product into a hydrogen peroxide solution, controlling the pH value to be 10-11, bleaching at 70-75 DEG C, and drying through a fluidized bed to obtain the cellulose. The cellulose, hemicellulose and lignin extracted by the method are high in purity, the yield of the bamboo waste cellulose is up to 73.2%, the purity is up to 91.6%, and the method has potential application value.

The invention belongs to the technical field of microorganisms, and particularly relates to sphingomonas fluorescens and application thereof in lignin degradation. The invention discloses a sphingomonas fluorescens H39, the preservation number of the sphingomonas fluorescens H39 is CCTCC (China Center for Type Culture Collection) No: M 2025936, and the sphingomonas fluorescens H39 is preserved in the China Center for Type Culture Collection on April 29, 2025. The strain not only has remarkable peroxidase activity (85.34 U / mL), but also can stably grow under the condition of multiple nitrogen sources (such as ammonium sulfate, peptone, yeast extract and the like), the culture period is short (3-5 days), and the lignin degradation rate of agricultural wastes such as corn straw and the like is remarkably improved. The invention finds that a new microbial resource is provided for biodegradation of lignin, and resource utilization of agricultural wastes and development of environmental protection industry are expected to be promoted.

The invention relates to the technical field of medicinal fungus cultivation, and discloses a poria cocos low-carbon cultivation method using Pinus yunnanensis stumps, which comprises the following steps: drilling holes in the surfaces of the Pinus yunnanensis stumps; preparing and using a biomass guiding rope carrying a signal synergistic activating microbial inoculum to carry out directional activating inoculation; after in-situ activation culture, a poria cocos strain is inoculated for cultivation. The signal synergistic activating microbial agent is prepared by compounding efficient lignin degrading bacteria, combined nitrogen-fixing bacteria, lignin degradation product analogues and poria cocos mycelium metabolism extracts. And the biomass guide rope guides the activated microbial inoculum into the stump. The invention further discloses a step of continuously cultivating fast-growing edible fungi on stump residues after harvesting of poria cocos, lignin is effectively degraded and biological nitrogen fixation is synchronously performed through in-situ activation of microorganisms, the carbon-nitrogen ratio of a matrix is improved, the planting period of poria cocos is shortened, the unit yield and the content of effective components are increased, and the yield of poria cocos is increased. And gradient utilization of the stump biomass is realized.

The invention relates to a corn straw pretreatment method with synergy of composite fungi and stepped temperature control, and belongs to the technical field of agricultural waste resource utilization. In order to solve the problems of unbalanced enzyme activity, strain competition and high inoculation cost during corn straw pretreatment by using composite fungi, the invention provides a corn straw pretreatment method based on composite fungi synergistic stepped temperature control, which comprises the following steps of: crushing and sieving corn straws, and adding a fermentation culture medium to adjust the moisture content to obtain corn straw fermentation liquor; the method comprises the following steps: inoculating a composite fungal spore solution into a corn straw fermentation solution, and culturing for 15 days by adopting step temperature control to finish pretreatment; the enzymatic activities of different fungi are accurately activated through staged temperature control, the degradation efficiency of the corn straw is improved through the synergistic effect, and the cellulose degradation rate is improved by 21.8% compared with that of constant-temperature culture; and the lignin degradation rate is improved by 36.0%. The stepped temperature control ensures that each enzyme works at the optimal temperature, the catalytic efficiency is maximized, the enzymolysis rate is improved, the pretreatment time is shortened, and the treatment cost is reduced.

The invention relates to application of bacillus megaterium MZ-01 and a biological agent thereof in improving the quality and physical characteristics of cigars, and aims to solve the technical problem that lignin degradation and mature microbial fermentation systems are lacked in the current cigar fermentation field. The strain MZ-01 is named as Bacillus megaterium MZ-01 in taxonomy, and the preservation number of the strain MZ-01 is CCTCC (China Center for Type Culture Collection) NO: M 2025985. The bacillus megaterium MZ-01 can effectively degrade lignin and total sugar in cigar tobacco leaves, improve the sensory quality and physical characteristics of cigars (increase the tension value of the cigar tobacco leaves) and improve the industrial usability of the cigars, and the bacillus megaterium MZ-01 has the characteristics of simple process, safety, high efficiency and low treatment cost when being used for processing the cigar tobacco leaves, and has a wide application prospect. The method has important significance on development of a tobacco fermentation technology.

The invention relates to a method for preparing aromatic aldehyde by degrading lignin, which comprises the following steps: firstly, extracting lignin from different sources by an organic solvent method, dissolving the lignin in an organic solvent containing an acidic additive, introducing ozone to carry out ozone oxidative degradation, and then reducing ozone oxide by adopting a reducing agent aqueous solution to obtain the aromatic aldehyde. Finally, the lignin is converted into aromatic aldehyde compounds such as p-hydroxy benzaldehyde, vanillin and syringaldehyde. Compared with the prior art, the method has the advantages that the operation is simple and convenient, the reaction condition is mild, and the method is green and environment-friendly; acid is added into a reaction system, so that the lignin conversion rate and the aromatic aldehyde selectivity are effectively improved. The invention provides a new thought for efficient utilization of lignin, and also provides a feasible technical route for production of high-added-value chemicals.

The application discloses a perovskite catalyst for lignin degradation and a preparation method thereof. Para-carboxybenzaldehyde and pyrrole are used as raw materials to obtain symmetrical porphyrin, then KH550 is added to make the amino group on the KH550 and the carboxyl group on the substrate dehydrate and condense to obtain an intermediate 1, the intermediate 1 is reacted with cobalt chloride hexahydrate to form a metal complex, then modified perovskite matrix and deionized water are added, and the siloxane on the metal complex is hydrolyzed and grafted on the surface of the modified perovskite matrix to obtain the perovskite catalyst. The perovskite catalyst has higher stability due to the multilayer coating. When the perovskite catalyst is irradiated to form an oxidation-reduction system of an electron-hole pair, the oxidant adsorbed on the catalyst is changed into a free radical to act on the lignin substrate, so that the lignin is degraded. The surface metal porphyrin can increase the redox potential of the catalyst, so that the catalyst has a smaller dosage compared with a traditional catalyst.

This invention discloses a method for synthesizing a temperature-controlled heterogeneous heteropolyacid catalyst and its application in lignin conversion, belonging to the field of chemical degradation technology. Firstly, Mo atoms are partially replaced with Al and V atoms to synthesize the homogeneous heteropolyacid H5PMo. 11 Al 0.5 V 0.5 O 40 Then, choline chloride is added via ion exchange to synthesize a temperature-controlled heterogeneous polyacid catalyst, Ch. n H 5‑ n PMo 11 Al 0.5 V 0.5 O 40 Where n is the number of Ch atoms, and n is an integer, the heterogeneous temperature-controlled heteropolyacid catalyst can be applied to the degradation of lignin. This invention adjusts the Lewis acidity and homogeneity of the catalyst by adding Al atoms and choline chloride ionic liquid, thereby affecting the catalyst's reactivity and improving the degradation yield of lignin degradation products. The conditions are mild, the operation is simple, and it has positive significance for improving the yield of lignin degradation products.

This invention relates to the field of genetic engineering technology, and more particularly to an engineered bacterium with high substrate utilization efficiency, its construction method, and its applications. The method includes overexpressing laccase and lignin peroxidase in two monokaryotic Ganoderma lucidum strains, respectively, and then hybridizing the two monokaryotic strains to form a new Ganoderma lucidum. Ganoderma lingzhi MKLGE253252, depositary institution: China General Microbiological Culture Collection Center; address: No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing; deposit date: March 11, 2026; accession number: CGMCC No. 42594. It synergistically enhances lignin degradation capacity, mycelial utilization efficiency, and growth capacity, and significantly improves the strength of mycelial fiber sheath. This invention fundamentally solves the problems of low lignin utilization efficiency, slow mycelial growth, and insufficient mycelial sheath strength in existing technologies, providing a completely new technical path for the production of bio-based leather.

The application discloses a low-temperature rotting-promoting composite microbial inoculum, a high-efficiency in-situ straw returning method and application, and belongs to the technical field of microbial inoculums. The low-temperature rotting-promoting composite microbial inoculum comprises Stenotrophomonas maltophilia JGDQ29-2, Bacillus JX-7, Weissella covesi zqw90, Weissella paramesenteroides zqw26 and Neurospora crassa N7, and the mass ratio of the viable bacterial numbers of the Stenotrophomonas maltophilia JGDQ29-2, the Bacillus JX-7, the Weissella covesi zqw90, the Weissella paramesenteroides zqw26 and the Neurospora crassa N7 is 1-2:1-2:1-2:1.5-2:2.5-3. The application realizes the synchronous high-efficiency degradation of each component of the straw, improves the overall rotting efficiency and improves the soil nutrient components by screening high-efficiency cellulose, hemicellulose and lignin degradation strains and constructing a composite microbial system with multiple bacteria symbiosis and function complementation.

The invention discloses laccase-producing penicillium and application thereof, and relates to the technical field of microbial fermentation. The invention provides a laccase-producing natural penicillium, the laccase-producing natural penicillium is identified and named as Penicilliumucsense, a culture medium of the laccase-producing natural penicillium is optimized, and culture conditions for efficiently producing laccase are obtained. Compared with an engineering strain for biologically synthesizing laccase, the strain has relatively strong environmental adaptability, laccase synthesis can be naturally performed under wide natural conditions, the genome of the strain is relatively stable, potential risks of exogenous genes do not exist, and the safety is relatively high. The penicillium is short in growth cycle, high in yield in the fermentation process and high in lignin degradation activity. The strain Penicilliumuse is applied to degradation of phenol-polluted soil, the toxicity reduction effect is remarkable, the degradation cost is reduced, the degradation period is shortened, and an excellent strain is provided for production of composite oxidase systems such as laccase.

The application discloses a kind of microbial inoculum for improving humification efficiency of biological drying and rotting process and method thereof.The effective component of the microbial inoculum is composed of high-temperature-resistant strains, such as Saccharomonospora viridis, Pseudomonas taiwanensis, Microtus luteus, Bacillus licheniformis and Bacillus subtilis.The microbial inoculum provided by the application can significantly increase bacterial abundance when applied to the biological drying and rotting system of organic waste, accelerate the temperature rise and dehydration of the substrate in the biological drying stage, and make the system enter the high-temperature stage earlier.The lignin degradation rate is increased by more than 20%, the demethoxylation rate of lignin and the breaking rate of β-O-4 bond are increased, and more aromatic skeletons rich in phenolic hydroxyl groups are produced as key humic acid precursors for HA synthesis.The lignin-protein and polyphenol pathways are enhanced, and the seed germination index and humification ratio of compost products are significantly increased by more than 20%.

The invention discloses laccase and application thereof, and belongs to the field of bioengineering. The high-activity laccase Lac-11 is obtained through database mining, and compared with existing high-activity laccase BstCotA, the high-activity laccase Lac-11 has the following beneficial effects that 1, the enzyme activity is remarkably improved, the enzyme activity is improved by nearly 50% under the conditions that the temperature is 37 DEG C and the pH is 5.0, and the industrial enzyme cost is reduced; 2, the pH stability is higher, the enzyme activity retention rate is higher in the pH range of 5.0-8.0, and the method is suitable for wider acid-base environments. And 3, the thermal stability is better, the stability at the medium-high temperature interval of 50-80 DEG C is obviously higher than that of a control enzyme, and the method is suitable for an industrial high-temperature process. 4, the dye decolorization efficiency is higher, the decolorization rates of amino black 10B and crystal violet respectively reach 31% and 37%, and printing and dyeing wastewater can be efficiently treated. And 5, the lignin degradation capacity is outstanding, the degradation rates within 1 hour, 2 hours and 24 hours are 22%, 25% and 58% respectively, and pulping bleaching and biomass recycling are facilitated.

The invention belongs to the technical field of microbial metabolism engineering, and particularly relates to a lignin degrading bacterium, a separation method, a microbial agent and application of the lignin degrading bacterium in promoting biomass degradation. The strain is named as Trabulsiella odontobacteria L1, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.35692. The invention further discloses a preparation method of the strain. The invention provides a lignin degrading bacterium L1 which can degrade lignin under both an aerobic condition and an anaerobic condition, so that the degradation and conversion of biomass are promoted, and the yield is increased. Therefore, compared with the prior art, the lignin degrading bacterium L1 disclosed by the invention is unique in function, low in oxygen demand and beneficial to lignin degradation demands in different scenes.

The invention discloses a buffalo rumen-derived lignin degrading bacterium composite microecological preparation and application thereof, and relates to the technical field of biology. Active components of the buffalo rumen-derived lignin degrading bacterium composite microecological preparation comprise a buffalo rumen-derived lignin degrading bacterium combination; the buffalo rumen source lignin degrading bacterium composition is prepared from bacillus sorola desert, bacillus cereus and ochrobactrum pseudointermedium. The buffalo rumen source lignin degrading bacterium composite microecological preparation can obviously improve the digestion and absorption capacity of beef cattle, and especially has an obvious degradation effect on wood fiber substances which are difficult to decompose, so that the feed utilization rate is increased, and the feeding cost is reduced.