82 results about "Lignin degradation" patented technology

The invention belongs to the field of biotechnology and environmental governance, and particularly relates to a manganese peroxidase mutant based on high-throughput screening modification and application of the manganese peroxidase mutant to straw degradation. Aiming at the problems of low natural secretion amount, insufficient specific activity, complex preparation process and the like of the existing manganese peroxidase, the method comprises the following steps: firstly, constructing engineering bacteria based on an escherichia coli exocrine system, constructing a mutant library through error-prone PCR in combination with a directed evolution technology, and performing high-throughput screening by utilizing an ABTS colorimetric method; the recombinant manganese peroxidase mutant with high catalytic activity is obtained, and the enzyme activity is improved by 1.29 times compared with that of a wild type. Furthermore, recombinant engineering bacteria are constructed on the basis of a bacillus subtilis exocrine system, and efficient secretion of the mutant enzyme is achieved. The mutant enzyme preparation is used for wheat straw degradation, and the lignin degradation rate reaches 41.37% (improved by 30.09% compared with that of a wild type). The method is simple and convenient in process, low in cost and remarkable in effect, and has a wide industrial application prospect.

The invention solves the problem that the fermentation effect is affected due to insufficient exchange of upper and lower layers of straws in a fermentation tank, and relates to the field of straw fermentation, in particular to fermentation equipment for degrading and treating the straws by using biological enzyme and lignin. The pipeline assembly is used for dispersing biological enzyme powder and lignin powder into the straw surface layer in the fermentation tank, a ventilation assembly is installed at the extending end of the pipeline assembly, a stirring assembly is arranged in the fermentation tank and is in transmission connection with the pipeline assembly, and the stirring assembly comprises a stirring shaft and a plurality of stirring blades; the stirring shaft is in transmission connection with a driving motor at the top of the fermentation tank; multiple layers of spiral sheets are arranged on the outer side of the stirring shaft. According to the invention, radial straw flow in the horizontal plane in the fermentation tank is promoted, exchange of upper and lower layers of straw can be promoted, it is ensured that the straw is uniformly stirred in the full range of the fermentation space in the fermentation tank, and finally, the fermentation effect and uniformity of the straw in the fermentation tank are effectively improved.

The invention relates to an Acinetobacter variants L49. The Acinetobacter variants L49 can rapidly and stably grow by utilizing a cheap culture medium, the fermentation period is short, and the lignin degradation efficiency is high. The strain can be used for degrading straws by a biological method, improves the utilization rate of the straws, and has very high economic value and ecological value.

The invention discloses a salt-alkali-tolerant growth-promoting ectopseudomonas DHS-HJ-IAA-2 strain and an application thereof, and belongs to the technical field of microorganisms. According to the invention, a strain of salt-resistant, alkali-resistant and drought-resistant pseudomonas sp. DHS-HJ-IAA-2 is screened, and the pseudomonas sp. DHS-HJ-IAA-2 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36164. Experimental results show that the ectopseudomonas DHS-HJ-IAA-2 has the functions of producing IAA, fixing nitrogen, dissolving potassium, degrading cellulose and lignin, inhibiting phytopathogen and the like, and can remarkably improve the bacteria abundance of saline-alkali soil, improve soil fertility and promote plant growth in a saline-alkali environment. The invention provides a new strain resource and a technical means for improving saline-alkali soil and planting crops on the saline-alkali soil, and realizes improvement of the saline-alkali soil from two aspects of soil-crop-microorganism comprehensive action and microorganism-plant synergistic action.

The invention relates to the field of microorganisms, in particular to bacillus subtilis, a fungicide and application of the fungicide. The invention discloses a bacillus subtilis strain, which is classified and named as bacillus subtilis, has a strain number of GXGD-YZ-2483, is preserved in Guangdong Microbial Culture Collection Center on March 13, 2025, and has a preservation number of GDMCC No: 66014. The bacillus subtilis can tolerate the high temperature of 70-90 DEG C, has excellent self-heat production performance, and can produce lignin degrading enzymes (laccase, manganese peroxidase and / or lignin peroxidase) and inhibit the growth of pathogenic bacteria (escherichia coli, salmonella, clostridium perfringens and / or aspergillus flavus). When the bacillus subtilis or the microbial inoculum prepared from the bacillus subtilis is used for biologically composting organic wastes, a lignin barrier can be efficiently cracked by secreting a lignin degrading enzyme system, lignin degradation, nitrogen retention and pathogenic bacteria inactivation can be synchronously realized by virtue of high temperature resistance, self-heat production characteristics and enzyme activity stability, the composting period is shortened, and the yield of the organic wastes is improved. The humification quality is obviously improved.

The present invention belongs to the field of microbial technology and relates to a strain of Bacillus subtilis subsp. and its application in degrading lignin. It is named Bacillus subtilis subsp. NBL-B12058, deposited in the China Center for Type Culture Collection on October 17, 2022, with the deposit number CCTCC NO: M20221574, and the deposit address is Wuhan University, Wuhan, China. The Bacillus subtilis subsp. provided by the present invention has a good degradation effect on lignin in crop straws and the black water of paper mills.

An enzymatic bioremediation reactor device for the accelerated composting of lignocellulose material, consisting of a sealed, insulated container, a mechanical pretreatment unit and an automated laccase enzyme supply system.

The invention relates to an application of a pelagic fermentation broth, and the pelagic fermentation broth is prepared by the following steps: inoculating pelagic HE39 into a culture medium, carrying out fermentation culture, centrifuging, and taking the supernatant; the preservation number of the Phlebiopsis pilaris HE39 is CGMCC (China General Microbiological Culture Collection Center) No.41168, and the preservation number of the Phlebiopsis pilaris HE39 is CGMCC No.41168. The fermentation liquor can be used for degrading lignin and cellulose, has good degradation capacity on lignin in ground cover combustible materials, has good laccase activity, lignin peroxidase activity and manganese peroxidase activity, and can improve the weight loss rate of the combustible materials in forest land.

This invention belongs to the field of textile engineering technology and relates to a method for improving the performance of regenerated lignocellulose fibers based on lignin degradation. Corn stalks are mixed with an ionic liquid and tyrosine, then heated to dissolve, obtaining a spinning solution. The spinning solution is then subjected to wet spinning, and the spun fibers are solidified in a coagulation bath to obtain regenerated fibers. Compared with existing technologies, this invention uses corn stalks as the spinning raw material, which is widely available and inexpensive. Furthermore, this invention can achieve complete dissolution of the stalks and effectively remove lignin, reducing the lignin content in the regenerated fibers and improving their performance.

The present invention discloses Trichoderma asperellum ZC3 and its application in the degradation of Zanthoxylum bungeanum branches. This strain has a fast growth rate and strong degradation ability. In a liquid filter paper strip culture medium, after culturing at 28 °C for 7 days, the filter paper strip completely disintegrates; in a solid culture medium of Zanthoxylum bungeanum branch powder, after culturing at 28 °C for 40 days, its lignin degradation rate for Zanthoxylum bungeanum branches reaches 78.11%, and the degradation rates of cellulose and hemicellulose reach 82.58% and 75.84% respectively. Under a scanning electron microscope, obvious fiber breakage and structural disintegration phenomena can be seen in the Zanthoxylum bungeanum branches, showing a high degradation ability. In addition, the lignin-degrading bacterium ZC3 has good tolerance to the leaching solution of Zanthoxylum bungeanum branches.

The invention discloses a bacterium for degrading lignin under a low-temperature alkaline condition and application of the bacterium, and relates to a bacterium for degrading lignin and application of the bacterium, the bacterium for degrading lignin under the low-temperature alkaline condition is Pseudomonas reidholzensis PR-6, the bacterium is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC 65470. The invention also discloses application of the catalyst in degradation of lignin under low-temperature alkaline conditions. According to the present invention, the Pseudomonas reidholzensis strain PR-6 is screened from the lignin-rich soil so as to obtain the PR-6 strain with the efficient degradation ability, and the PR-6 can secrete the enzymes related to the lignin degradation; the method disclosed by the invention has a beneficial effect of degrading lignin by the PR-6 strain under the low-temperature alkaline condition.

A strain Y26 for degrading lignin, characterized in that the strain Y26 is Acinetobacter baumannii (ATCC 19606T) Acinetobacter baumannii ), preserved in the China Center for Type Culture Collection, located at No. 299, Bajiyuan Road, Wuchang District, Wuhan City, Hubei Province, China, with a preservation number of CCTCCM20242944 and a preservation date of December 30, 2024. The Acinetobacter baumannii Y26 in the present application has excellent ability to degrade lignocellulose, with a Lac enzyme activity of 60.38±2.5 U / L, a Mnp enzyme activity of 2612.14±25 U / L, a Lip enzyme activity of 1460.62±14 U / L, and a lignin degradation rate in corn stalks of 38.93%. In the present application, the corn stalks are pretreated by using the Acinetobacter baumannii Y26 in combination with maleic acid, so that the cellulose conversion rate of the corn stalks reaches 87.41%, the glucose yield reaches 0.668 g / g of stalks, and the prepared saccharification liquid has small inhibition effect on microbial fermentation, effectively improving the ethanol yield.

The present invention relates to a method for cleanly and efficiently separating acid hydrolyzed fiber residue components and performing in-situ polymerization reaction of residual liquid generated in the separation process at room temperature to prepare a flocculant for sewage purification. The method comprises using a low eutectic solvent (DES) method to separate high-purity lignin and cellulose components from the acid hydrolyzed fiber residue, and making full use of the characteristics of the DES residual liquid rich in lignin degradation products after multiple cycles, establishing an autocatalytic system by adding a trace amount of initiator, and performing in-situ polymerization reaction at room temperature to prepare a high-performance flocculant. The present invention is a breakthrough in that the DES residual liquid with severely reduced pretreatment effect is used as a raw material to perform in-situ polymerization reaction at room temperature through an autocatalytic system to prepare an efficient sewage treatment agent, and the high-value utilization of the acid hydrolyzed fiber residue is achieved under the principle of no waste.

The invention discloses a buffalo rumen source lignin and cellulose degrading bacterium microecological preparation as well as a preparation method and application thereof, and relates to the technical field of microorganisms. The active ingredient of the microecological preparation comprises bacillus sorola desert, and the preservation number of the bacillus sorola desert in the China Center for Type Culture Collection (CCTCC) is CCTCC NO: M2025512. According to the invention, a strain of bacillus sorola, which has lignin degradation ability and can degrade cellulose, is screened from a rumen solid-liquid mixture sample obtained from a buffalo rumen fistula, and is developed into a microecological preparation. Experimental verification shows that the degradation rate of the strain to sodium lignin sulfonate in 24 hours reaches 9.54%, the degradation rate of the strain to straw in 3 days can reach 8.6% or above, and in an artificial simulation rumen test, lignin in the straw can be remarkably degraded, and meanwhile, the fiber utilization value of the straw is effectively improved.

The invention discloses a method for catalyzing decarboxylation halogenation of renewable carboxylic acid by using non-coenzyme dependent peroxidase. Comprising the following steps: adding a substrate, peroxidase, hydrogen peroxide and potassium halide into a buffer solution containing an organic solvent, and stirring and reacting at normal temperature to obtain the halide. The substrate is monomer benzoic acid obtained after lignin is degraded. The peroxidase does not depend on cofactors and is wide in source and low in production cost. The method is simple in preparation process, mild in reaction condition, high in substrate conversion rate and good in reaction selectivity. And the downstream treatment process is simple.

The present invention relates to a strain of Serratia marcescens GZAUS341020 with high efficiency in degrading lignin and its application. A strain of Serratia marcescens GZAUS341020 with high efficiency in degrading lignin was isolated from environmental samples. This strain is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC NO: 64617. The obtained Serratia marcescens strain has a good lignin degradation rate under different temperature conditions, and also has good cellulase, lipase, and amylase activities, and can be used for the biodegradation of agricultural straw.

The invention discloses an efficient lignocellulose degradation compound strain and application thereof in producing methane by fermenting straws. The lignocellulose degradation compound strain is prepared from phanerochaete chrysosporium, oyster mushroom, phoenix mushroom and talaromyces pinophilus. When the composite strain is used for pretreating straws, the degradation rate of cellulose in the straws reaches 77.86%, the degradation rate of hemicellulose in the straws reaches 21.01%, and the degradation rate of lignin in the straws reaches 34.85%; when the pretreated straw and the excrement are subjected to anaerobic fermentation together, compared with a control group, the total gas production rate is increased by 86.28%, and the methane production rate is increased by 98.95%. Therefore, the invention provides a green and efficient straw resource utilization technology so as to synchronously realize the purposes of waste reduction, energy recovery and fermentation residue resource utilization.

The invention relates to BiOX (X = halogen elements Br, I and Cl)-loaded PVA hydrogel and a preparation method and application thereof, the BiOX (X = halogen elements Br, I and Cl)-loaded PVA hydrogel is composed of Bi (NO3) 3.5 H2O, KX (X = Br, I and Cl) and a PVA solution, and PVA / BiOX composite hydrogel is formed; pVA / BiOX hydrogel is used as an efficient carrier, effective loading and dispersion of BiOX nanoparticles are realized through hydrogen-rich bonds on the surface, the pore structure of the hydrogel is enhanced through foaming crosslinking, the diffusion rate of lignin molecules is increased, and thus the performance of photocatalytic degradation of lignin is enhanced. The catalyst is low in preparation cost, mild in preparation condition, convenient to operate and convenient for large-scale production in industry. The PVA / BiOX catalyst provided by the invention is applied to the field of photocatalysis, has good photocatalytic degradation performance on lignin, provides an efficient and environment-friendly method for lignin degradation, and has a wide application prospect.

The invention belongs to the technical field of composite strains, particularly relates to a high-lignin straw fermentation composite microbial agent, and a preparation method and a fermentation process thereof, and aims to solve the problems of low lignin degradation efficiency, long period and high cost of a single microorganism in the prior art. According to the invention, a composite synergistic system of coriolus versicolor, bacillus subtilis and pichia pastoris is innovatively adopted, and efficient degradation is realized through a mechanism that the coriolus versicolor secretes a lignin degrading enzyme system, the bacillus subtilis assists cellulose decomposition and the pichia pastoris optimizes a microenvironment; the preparation method of the complex microbial inoculant comprises the following steps: culturing various strains in a proper culture medium to a specific growth state, and mixing according to an optimized volume ratio; the fermentation process relates to high-lignin straw pretreatment, inoculation, sealed fermentation under control conditions and regular ventilation, the lignin degradation rate can be increased, the feed nutritional value can be improved, the relative feeding value and quality can be improved, and economic and environment-friendly technical support is provided for resource utilization of agricultural by-products.

The invention relates to a thermophilic carbon monoxide streptomyces and a complex microbial inoculant, a preparation method and application thereof, and belongs to the technical field of microorganisms, the classification name of the strain is thermophilic carbon monoxide streptomyces, the name of the strain is G-4-7, and the preservation number of the strain is CGMCC No.36417. The efficient lignin degradation complex microbial inoculant can be prepared from the thermophilic carbon monoxide streptomyces, Brevibacillus agri G-5-2 and Chaetomium thermophilum by using the thermophilic carbon monoxide streptomyces, the name of the strain is G-4-7, and the preservation number of the strain is CGMCC No.36417. The complex microbial inoculant can efficiently degrade bagasse lignin in a high-temperature environment, enzyme systems of all strains are complementary, the degradation efficiency is synergistically improved, resource utilization of bagasse rich in lignin can be promoted, and a technical basis is provided for a green treatment mode of agricultural waste.

The present invention discloses a method for mechanochemical enzyme-coupled lignin degradation. The specific operation process is to place lignin powder, a solid-phase reagent, and zirconium oxide beads in a polytetrafluoroethylene ball mill, perform a co-grinding reaction in the ball mill, add an enzyme after ball milling, add a solvent for stirring, and reflux reaction. After the reaction, the mixture is dissolved in water and extracted with ethyl acetate. The insoluble residue is centrifuged, washed with ethyl acetate, and vacuum dried for detection and analysis. The present invention has the advantages of a short process route, low reagent usage, simple operation, low cost, low pollution, low molecular weight of the obtained product, and good degradation efficiency. It is a lignin degradation method with good promotion and application prospects.

The invention belongs to the technical field of microorganisms, and particularly relates to a thermophilic strain B-60-8 for straw degradation and decomposition and application of the thermophilic strain B-60-8. The thermophilic strain B-60-8 is classified and named as thermophilic thermophilic strain B-60-8, and is preserved in China General Microbiological Culture Collection Center (CGMCC) on August 19, 2025, and the preservation number is CGMCC No.35676. The invention aims to obtain a single-function strain which can adapt to a high-temperature period of straw composting and efficiently crush lignin by utilizing H2O2 by establishing a straw decay promoting strategy oriented to H2O2 production capacity and verifying the high efficiency of the strain in straw degradation in a high-temperature environment, so that the problems of poor high-temperature adaptability of a microbial agent and low lignin degradation efficiency are solved, and the application prospect is wide. And a new microbial resource is provided for the practical application of straw decomposition and biological fertilizer preparation.

The invention discloses a lignin-formaldehyde adhesive as well as a preparation method and application thereof, and belongs to the technical field of efficient utilization of lignin. According to the invention, lignin is pyrolyzed into a micromolecular aromatic compound, and then a lignin liquid phase depolymerization product is mixed with a catalyst to prepare the self-crosslinking adhesive depending on a lignin degradation product. The lignin-formaldehyde adhesive provided by the invention has the advantages of low cost, high bonding strength and the like.

The invention discloses a method for preparing biomass oil by catalytic degradation of lignin, and belongs to the field of biomass resource utilization. The method comprises the following steps: step 1, pretreatment of lignin: primarily depolymerizing lignin macromolecules and generating part of aldehyde enzymolysis products; 2, collection of a pretreatment product: filtering the turbid liquid and solid residues by using a lignin degradation device; 3, catalysis of lignin and products thereof: catalyzing degradation of lignin through hydrogenation reaction; and 4, biomass oil extraction: extracting and drying to prepare the biomass oil. The method is novel and effective, lignin macromolecules are pretreated through biological enzyme, lignin is depolymerized, interfering alkali metal in lignin is removed through an acidic enzymolysis solution environment, then lignin is thoroughly degraded through hydrogenation reaction catalyzed by a metal catalyst, an enzymolysis product is reduced into biomass oil, the reaction conditions of the degradation reaction are reduced, and the yield of the biomass oil is increased. The production cost is reduced, and the degradation efficiency is optimized.

The application discloses a Talaromyces with multi-oxidase synergistic expression capacity and a fermentation method and application thereof. Talaromyces sp. The Talaromyces (Talaromyces autumnalis) Ta-lac is preserved in the China General Microbiological Culture Collection Center on July 11, 2025, and the preservation number is CGMCC No. 42125. Compared with the engineering strain for biosynthesis of laccase, the strain has strong environmental adaptability, can naturally synthesize laccase, and has relatively stable genome, no potential risk of exogenous genes and relatively high safety. The Talaromyces has a short growth cycle, high yield of laccase, nitropropane dioxygenase, hydrogenase coenzyme dioxygenase and squalene monooxygenase and high lignin degradation activity in a fermentation process. The Talaromyces Ta-lac is applied to degradation of toxin dye wastewater, and has remarkable detoxification and decolorization effects.

The invention discloses bacillus tequilensis with lignin degradation and black shank antagonism functions and application of the bacillus tequilensis JZF3, the bacillus tequilensis JZF3 is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation date is June 9, 2025, and the preservation number is CGMCC No.34784. The bacillus tequilensis JZF3 can be used for degrading lignin and antagonizing black shank. The bacillus tequilensis JZF3 has the functions of lignin degradation and shin disease antagonism, and meanwhile, a metabolite of a lignin degradation solution of the bacillus tequilensis JZF3 has the effect of promoting the growth of plants, so that the bacillus tequilensis JZF3 has a relatively high market prospect.

The invention discloses a laccase mutant and application thereof, the mutant is obtained by mutating wild laccase BstCotA from bacillus subtilis, the amino acid sequence of the mutant is as shown in SEQ ID NO.1, and the mutant is mutated into at least one of E186A, N65G, D185G and N311G. The invention further discloses a preparation method of the laccase mutant. Compared with a wild type, the mutant has the advantages that the catalytic activity is obviously improved, and the relative enzyme activity is higher under the conditions that the temperature is 37 DEG C and the pH value is 5.0; the pH stability and the thermal stability are enhanced, and the activity in the range of 40-90 DEG C exceeds 75%; the dye decolorization efficiency is higher, the decolorization rate of amino black 10B reaches 28%-35%, the decolorization rate of crystal violet reaches 35%-45%, and complex printing and dyeing wastewater can be efficiently treated; the lignin degradation capacity is better, the degradation rates within 1 hour and 2 hours are higher than those of the wild type, and pulping bleaching and biomass resource utilization are facilitated.

The invention relates to a natural enzyme-nano enzyme hybrid system and application thereof in lignin degradation, which comprises the following steps: mixing nano enzyme, natural enzyme and a phosphate buffer solution to obtain a mixed system, and regulating the pH value of the mixed system to 3.0-8.5 by using hydrochloric acid or a NaOH solution to obtain the natural enzyme-nano enzyme hybrid system; the method comprises the following steps: adding lignin into a natural enzyme-nano enzyme hybrid system to enable the final concentration of the lignin in the system to reach 1g / L, adjusting the temperature to 20-40 DEG C, placing the system in a shaking table, and carrying out oscillation reaction at 150-250rpm for not less than 24h; after the reaction is finished, centrifugation is conducted for 2-5 min at the speed of 10000-12000 rpm, supernate is taken for lignin degradation rate determination and GC-MS product analysis, degradation of lignin under the mild condition is achieved, the proportion of aromatic compounds in degradation products is increased, and a greener alternative scheme is provided for high-value utilization of lignin.

The invention discloses a resource utilization method of bamboo waste materials, which comprises the following steps: S1, washing the bamboo processing production waste materials until the water content of the bamboo processing production waste materials reaches 50-60%, then carrying out steam explosion treatment at 180-200 DEG C, obtaining steam explosion liquid containing lignin degradation products at a condensate interface, and recycling the steam explosion liquid containing lignin degradation products at the condensate interface; steam explosion solids containing cellulose are obtained in the material collecting cavity; s2, loading the steam explosion solid into an acid and alkali resistant filter bag, transferring into an enzymolysis system, adding a buffer solution into the enzymolysis system, and carrying out an enzymolysis reaction at 45-50 DEG C to obtain an enzymolysis product; and S3, transferring the enzymolysis product into a hydrogen peroxide solution, controlling the pH value to be 10-11, bleaching at 70-75 DEG C, and drying through a fluidized bed to obtain the cellulose. The cellulose, hemicellulose and lignin extracted by the method are high in purity, the yield of the bamboo waste cellulose is up to 73.2%, the purity is up to 91.6%, and the method has potential application value.

The invention discloses bacillus subtilis and application thereof, and belongs to the technical field of wood fiber pretreatment. The method comprises the following steps: inoculating bacillus subtilis FM-63 into a unique carbon source liquid culture medium of a lignin or lignocellulose raw material, degrading the lignin or lignocellulose raw material under the condition of slightly dissolved oxygen, and applying the bacillus subtilis FM-63 to pretreatment enzymolysis synergy for degrading the lignocellulose. Under the micro-aerobic condition, the lignin degradation rate of the corn straw is 17.69%, and the enzymolysis efficiency of the pretreated residues is improved by 20.36% compared with that of the raw materials.