35 results about "Cellulose breakdown" patented technology

The invention belongs to the technical field of waste resource utilization, and provides a method for enhancing anaerobic digestion to produce methane. The invention provides a research and development concept of dynamically regulating and controlling nano bubble water injection in stages. The core breakthrough points of the research and development concept comprise: 1) time sequence optimization; based on the pH value of an anaerobic digestion system, H2-NBW or O2-NBW is injected in stages, and the anaerobic digestion hydrolytic acidification stage and the methane production stage are regulated in a targeted manner. O2-NBW is injected in the hydrolytic acidification stage to promote decomposition of protein and cellulose in a substrate, H2-NBW enriched hydrogen nutrition type methanogenesis is switched in the methanogenesis stage, and a hydrogen nutrition type methanogenesis way is enhanced. And (2) when the pH is more than or equal to 6.5 and less than or equal to 7.5 in a neutral safe region, dynamically adjusting the gas type of the nano bubble water by combining the ORP of the anaerobic digestion system, thereby realizing the accurate gradient control of the oxidation reduction potential and improving the methane yield.

The invention discloses streptomyces bulbus as well as a multifunctional fungicide and application thereof. According to the invention, firstly, a strain of Streptomyces tauricus zhanshen-LZ-12 is separated and screened from straw returning soil, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.34456. The invention also discloses a preparation method of the Streptomyces tauricus zhanshen-LZ-12. The strain has the functions of degrading cellulose, decomposing protein, fixing nitrogen, resisting salt, degrading phenolic acid autotoxic substances (benzoic acid, p-hydroxybenzoic acid and cinnamic acid) and the like. Bacterial powder obtained after fermentation of the bacterial strain is uniformly mixed with bacillus bacterial powder to obtain the multifunctional bacterial agent, and when straw returning or composting is carried out, the multifunctional bacterial agent is adopted, straw decomposition can be accelerated, soil ecology can be improved, crop growth can be promoted, and the stress resistance of crops can be enhanced.

The invention belongs to the technical field of fermentation, and particularly relates to a method for preparing an enzyme through multiple fermentation of citrus fruits and the enzyme. The invention relates to a method for preparing enzymes through multiple fermentation of citrus fruits. The method comprises the following steps: S1, pretreatment of raw materials; s2, performing multiple fermentation; and S3, performing post-treatment on the enzyme. According to the present invention, through the complex enzyme synergistic pretreatment, the multi-strain compounding three-stage gradient fermentation and the low-temperature membrane concentration technology, the active components such as vitamin C, flavonoid and the like in the prepared enzyme are efficiently released and most of the active components are retained, the cellulose decomposition rate and the viable count of probiotics are significantly increased, the sensory evaluation is high, and the shelf life is long. The invention provides an innovative solution for industrial production of the citrus enzyme.

The invention discloses quick-acting inflammation-diminishing hemorrhoid cream and a preparation method thereof. Organic compost comprises the following components in percentage by mass: 10-20% of bean cakes, 8-15% of peanut cakes, 5-15% of wheat bran, 20-30% of leaves or green grass, 25-35% of dry cattle and sheep manure, 3-8% of brown sugar and 3-8% of a compound microbial agent containing cellulose decomposition bacteria and lactic acid bacteria. The preparation method comprises the following steps: mixing the raw materials, adjusting the water content to 50-60%, fermenting in a closed environment at 20-35 DEG C for 7-10 days, and periodically turning in the period. Carbon-nitrogen balance is optimized through the specific proportion of the plant source raw materials and the animal waste, the low-temperature anaerobic fermentation process is combined, the fermentation period is remarkably shortened, the humus conversion efficiency is improved, and the obtained compost is free of chemical additives and suitable for green agricultural production.

The invention relates to the technical field of soil improvement, in particular to a complex microbial inoculant suitable for straw organic fertilizer fermentation and a method for improving and fertilizing soil through the complex microbial inoculant. The complex microbial inoculant is formed by compounding a functional microbial inoculant and a carrier according to the mass ratio of 1: (3-5); the functional microbial agent is prepared by mixing lignin degrading bacteria, cellulose decomposing bacteria and functional auxiliary bacteria; all the components of the complex microbial inoculant have a synergistic effect, the fermentation function of the complex microbial inoculant can be greatly improved, the lignin degrading bacteria specifically break ether bonds (-O-) and carbon-carbon bonds (C-C) in a lignin three-dimensional network structure by secreting laccase, manganese peroxidase and the like, lignin is degraded into low-molecular aromatic compounds, and the low-molecular aromatic compounds can be used for degrading the lignin into low-molecular aromatic compounds. Physical wrapping on cellulose is removed, so that a straw fiber structure is converted into a loose structure which is easy to decompose from a lignin-cellulose complex.

The invention discloses a livestock and poultry breeding feed additive capable of reducing cost and improving effect. The livestock and poultry breeding feed additive is prepared from the following raw materials through compound fermentation: probiotic components: bacillus subtilis, clostridium butyricum and enterococcus; the enzyme preparation components comprise cellulose decomposition enzyme and xylanase; a fermentation medium: brown sugar and water; the traditional Chinese medicine auxiliary materials comprise wormwood, health sand and andrographis paniculata. The preparation method of the cost-reducing and effect-improving livestock and poultry breeding feed additive comprises the following steps of bacterium expanding culture, secondary fermentation and product treatment. The invention belongs to the technical field of livestock and poultry breeding, and particularly provides a livestock and poultry breeding feed additive and a preparation method thereof, and the livestock and poultry breeding feed additive can improve the intestinal microecological balance of livestock and poultry, improve the digestion and utilization rate of feed, enhance the immunity of livestock and poultry and reduce the breeding cost through scientific matching of probiotics, enzyme preparations, traditional Chinese medicine components and nutritional auxiliary materials.

This disclosure relates to a combination, including a composition, of cellulolytic microorganisms, wherein each cellulolytic microorganism produces a glycosylase, said combination comprising: a first cellulolytic microorganism that produces a cellulase classifiable by EC 3.2.1.4 or EC 3.2.1.91; and a second cellulolytic microorganism that produces a cellulase classifiable by EC 3.2.1.4, EC 3.2.1.91, EC 3.2.1.21, EC 3.2.1.52, EC 3.2.1.74 or EC 3.2.1.86. Also disclosed are related methods including to produce a simple carbohydrate from cell wall cellulosic material, and a ruptured cell wall, and to retrieve intracellular materials of the ruptured cell such as lipid or protein.

The invention belongs to the technical field of strigose hydrangea juvenile leaf active substance extraction, and provides a strigose hydrangea juvenile leaf active substance extraction method based on efficient cellulose decomposition bacteria, which comprises the following steps: collecting a soil sample, carrying out microorganism enrichment culture by taking strigose hydrangea juvenile leaf as a carbon source, separating and purifying a single strain, screening cellulose degradation dominant bacteria by adopting a Congo red staining method, and carrying out high-efficiency cellulose decomposition on the dominant bacteria. Then secondary screening is carried out through CMC enzyme activity and beta-glucosidase activity; with a water extraction method as a contrast, screening strains with the best effect of improving the active substances of the strigose hydrangea juvenile leaves; the influence of the inoculum size, the fermentation time and the material-liquid ratio on the extraction of the active substances is investigated through a single-factor experiment; on the basis of the constitution of the cell wall of the sweet tea, cellulose decomposition bacteria with efficient enzyme activity are screened and act on the sweet tea to damage the cell wall structure of the sweet tea, so that the release of active substances in the sweet tea is improved, the extraction rate of sweet tea flavone and sweet tea polyphenol is improved, and a green biological pretreatment technology is formed.

The application discloses a method for producing feed by using Gramineae plant waste, and comprises the following steps: S1, raw material pretreatment: after the Gramineae plant waste is dedusted, cut or crushed, water immersion and ammoniation treatment are carried out, so that the water content reaches 30-60%, and the ammonia addition amount is 3-5% of the dry weight; S2, gas phase blasting: the pretreated material is subjected to gas phase blasting, and the medium is selected from water vapor or non-reactive compressed gas; S3, microbial agent fermentation: a compound microbial agent containing at least two of lactic acid bacteria, yeast bacteria, bacillus and cellulose-decomposing bacteria is added to the blasted material, the water content is adjusted to 60-70%, and sealed fermentation is carried out for 3-7 days; S4, granulation: the fermented material is dried to have a water content of 12-18%, other raw materials are mixed after crushing, and the feed product is obtained through steam conditioning and pressing granulation. Through the cooperation of multiple processes, the method can significantly destroy the structure of the straw, improve the crude protein content and the feeding value, and the product is convenient to store and transport.

The invention relates to the technical field of biomass energy clean conversion, in particular to a power generation system and method based on biomass enzyme catalytic gasification coupling waste recycling, and the system comprises a low-temperature catalytic gasification module, an efficient power generation module and a full-amount recycling module. The method comprises the following steps: catalyzing cellulose and hemicellulose in straws to be decomposed into glucose and xylose in a low-temperature environment and under a micro-positive pressure condition by utilizing a compound preparation consisting of cellulase, ligninase and methanogens, and carrying out anaerobic fermentation to generate methane-containing biogas; biogas is subjected to desulfurization and dehydration pretreatment and then directly generates power through a proton exchange membrane fuel cell, gasification residues are synchronously and completely converted into compound fertilizer through the processes of neutralization, filter pressing, granulation and the like, the comprehensive utilization rate of energy is increased by 50% compared with that of traditional incineration power generation, and a single electric energy output mode is changed.

The present invention relates to a process for producing cellulolytic and / or hemicellulolytic enzymes by a cellulolytic and / or hemicellulolytic microorganism, said process comprising at least:a) a phase of growing the microorganism in the presence of at least one carbon-based substrate, followed byb) a phase of producing the enzymes in the presence of at least one inducing substrate,and also:c) a phase of preparing a carbon-based substrate comprising glucose and / or fructose, which carbon-based substrate is used in either and / or both of the growth phase a) and production phase b), said preparation phase comprising a step c1) of hydrolysis in an acidic aqueous medium, of sucrose to glucose and fructose.

The present invention relates to a method for recycling a cellulose-containing fiber product, and an object of the present invention is to provide a recycling method capable of regenerating a cellulose-based fiber having sufficient strength even in a cellulose material which is difficult to be recycled, such as a material deteriorated due to repeated use, a material having a reduced cellulose polymerization degree due to a decrosslinking agent treatment, a defunctionalizing agent treatment, or a decoloring treatment of a fiber product, or a material having a low polymerization degree of original cellulose itself, such as rayon or lyocell.SOLUTION: The method for recycling a fiber product containing cellulose as a main component includes at least a decomposition step of decomposing cellulose into sugar and a bacterial cellulose production step of producing bacterial cellulose derived from the fiber product using the sugar obtained in the decomposition step as a material.SELECTED DRAWING: None

The invention relates to the technical field of black fungus production, and discloses a black fungus production process, which comprises the following steps: S1, selecting raw materials of a black fungus bag, the raw materials comprising sawdust, bran, soybean meal, gypsum, light calcium carbonate and a pH regulator; the formula comprises the following components in parts by weight: 82-88% of wood chips; 10%-14% of bran; 2%-4% of soybean meal; 0.4%-0.6% of gypsum; according to the black fungus culture medium, the fermented wood chips are used for preparing the culture medium, in the fermentation process, part of macromolecular cellulose and hemicellulose are decomposed into micromolecular carbohydrates, decomposition, absorption and utilization of black fungus hyphae are facilitated, black fungus strains are rapidly planted after inoculation, plant diseases and insect pests can be reduced, the fungus culture time is shortened to 35-40 days, and the yield of the black fungus strains is increased. According to the method, the fermented wood chips are used for cultivation, the black fungus emergence is fast, the black fungus buds are neat, the black fungus mature period is consistent, the method is suitable for one-time harvesting of full-automatic black fungus harvesting, the obtained yield is the same as that of traditional cultivation, only 35-40 days are needed from bag arrangement to harvesting, and the labor production efficiency is improved.

本发明公开了一种低温条件下兼具玉米与大豆秸秆降解能力的复合菌剂及其制备方法应用。所述的复合菌剂由保藏编号为CGMCC No.42446的木霉菌（Trichoderma sp.）FS5以及保藏编号为CGMCC No.37567的铜绿假单胞菌（Pseudomonas chlororaphis subsp.）BNT4组成。其中木霉菌FS5能将纤维素分解成较短的纤维寡糖或纤维二糖，铜绿假单胞菌BNT4则可以利用这些中间产物作为碳源，从而避免了这些产物积累对木霉菌FS5产生反馈抑制，间接促进了整个降解过程的效率，从而形成一套高效率的秸秆降解“流水线”。本发明提供的复合菌剂为东北地区主粮作物秸秆的绿色有效处理提供了新的解决方案，对于促进土壤固碳固氮，提高土地肥力及实现农业可持续发展具有重要意义。

Provided is a method for recycling fiber products containing cellulose, whereby even cellulose materials that are difficult to recycle, such as materials deteriorated by repeated use, materials having a reduced degree of cellulose polymerization due to decrosslinking agent treatment, defunctionalization agent treatment, decolorization treatment, or repeated recycling of fiber products, and materials having a low degree of polymerization of original cellulose such as Rayon or Lyocell, can be regenerated into cellulosic fibers having sufficient strength.　A method for recycling fiber products containing cellulose as a main component comprises at least: a decomposition step for decomposing cellulose into sugar; and a bacterial cellulose generation step for generating fiber product-derived bacterial cellulose by using the sugar obtained by the decomposition step as a material.

The invention discloses a method for efficiently removing heavy metal ions in livestock and poultry manure, which adopts an eluting agent to form a stable chelate with the heavy metal ions through a coordination effect, realizes efficient total removal of the chelate from a manure solid phase, and breaks through the limitation that only the bioavailability can be reduced by a traditional passivation technology. The manure solid phase is subjected to neutral adjustment and then mixed with straw, a compound microbial agent composed of cellulose decomposition bacteria, nitrogen-fixing bacteria and phosphate solubilizing bacteria is inoculated, aerobic fermentation is carried out, and the high-quality organic fertilizer is prepared; leacheate containing heavy metal is subjected to electric-PRB combined treatment, the heavy metal is promoted to be enriched on a PRB material by utilizing electroosmotic flow and electrophoretic migration, and then phosphorus resources are recovered by a struvite crystallization process. According to the method, the removal rate of copper in the livestock and poultry manure is 96.2%, the removal rate of zinc in the livestock and poultry manure is 91.3%, the removal rate of cadmium in the livestock and poultry manure is 98.0%, the agricultural-grade organic fertilizer and the phosphorus recovery liquid are obtained, the process is flexible, the cost is low, industrialization popularization is easy, and the method is suitable for manure treatment of different livestock and poultry types and pollution levels.

The invention relates to the technical field of microorganism application, in particular to a complex microbial inoculant for rapid decomposition of garden waste and a preparation method and application of the complex microbial inoculant. The microbial strain is prepared by mixing and fermenting white-rot fungus fermentation liquor, cellulose decomposition bacteria fermentation liquor and bacillus fermentation liquor according to the volume ratio of (30-40%): (20-30%): (30-50%); the bacteria content of the white-rot fungus fermentation liquor is 1 * 10 <-1 > * 10 < cfu > / mL, the bacteria content of the cellulose decomposition bacteria fermentation liquor is 1 * 10 <-1 > * 10 < cfu > / mL, and the bacteria content of the bacillus fermentation liquor is 1 * 10 <-1 > * 10 < cfu > / mL. The problem that traditional composting consumes too long time is solved, the treatment efficiency is improved, the strain ratio and process parameters are optimized, the decomposition capacity on lignin, cellulose and other refractory components is enhanced, and efficient conversion of organic matter in waste is promoted.

The invention discloses a preparation method of a healthy and environment-friendly household lawn planting fertilizer, and belongs to the technical field of agricultural fertilizers. The invention relates to a preparation method of a healthy and environment-friendly household lawn planting fertilizer. The preparation method comprises the following steps: 1) efficiently converting kitchen garbage and garden waste by adopting a complex microbial inoculant (azotobacter chroococcum, thermophilic cellulose decomposition bacteria and candida tropicalis) in a specific ratio; 2) designing an intelligent response type core-shell coating structure, and realizing accurate controlled release of nutrients through the synergistic effect of a PHBV copolymer core layer, a modified starch interface layer and a temperature-sensitive nano composite shell layer; and 3) a safe combination of a bitter agent and a botanical insecticide is innovatively used. The product has the characteristics of quick start and long-acting supply, can obviously improve the soil environment, is completely biodegradable and pollution-free, and solves the problems of single function and serious pollution of the traditional fertilizer. The preparation process ensures stable product performance by optimizing fermentation parameters and an electrospray coating technology, and is particularly suitable for ecological maintenance requirements of family lawns.

The invention discloses a ruminant overwintering concentrate supplement feed and a production method thereof. The ruminant overwintering concentrate supplement feed comprises the following components in parts by weight: 60-90 parts of a basic raw material, 5-15 parts of a cold-resistant stress additive, 1-5 parts of a probiotic compound, 1-5 parts of a flavor improving component and 4-8 parts of a mineral supplement, the probiotic compound comprises lactic acid bacteria, saccharomycetes and cellulose decomposition bacteria; the flavor improving component comprises molasses, vanilla powder, an acidifying agent and citric acid; the mineral supplement comprises calcium carbonate, calcium hydrophosphate, sodium chloride and compound trace elements, wherein the compound trace elements comprise iron, zinc, selenium and manganese. The basic raw materials comprise energy raw materials and protein raw materials, the ratio of the energy raw materials to the protein raw materials is 2: 1, and the energy raw materials are selected from at least two of corn, barley, wheat, wheat bran and rice bran; the protein raw materials are selected from at least two of soybean meal, cottonseed meal, rapeseed meal and peanut meal. The invention relates to the technical field of feed processing, and particularly provides a ruminant overwintering concentrate supplement feed and a production method thereof.

The present invention relates to processes for producing fermentation products from starch-containing material, wherein an alpha-amylase and optionally a thermostable protease, pullulanase and / or glucoamylase are present and / or added during liquefaction, wherein a cellulolytic composition is present and / or added during fermentation or simultaneous saccharification and fermentation. The invention also relates to a composition suitable for use in a process of the invention.

This invention relates to the field of microbial technology, specifically disclosing a *Pantotheca acuminata* strain and its applications. A *Pantotheca acuminata* strain DSX-01 is provided. Through purification and isolation methods, a strain with good cellulose-decomposing ability was obtained, identified as *Pantotheca acuminata* DSX-01. Experiments detecting its cellulose-decomposing enzyme activity showed that *Pantotheca acuminata* DSX-01 has good cellulose-decomposing ability. It was deposited on March 6, 2025, at the China Center for Type Culture Collection (CCTCC), address: Luojia Mountain, Bayi Road, Wuchang District, Wuhan City, Hubei Province; accession number: CCTCC M 2025390. The *Pantotheca acuminata* DSX-01 strain was inoculated into a liquid culture medium to obtain a liquid strain. The *Pantotheca acuminata* strain or its fermentation broth was then inoculated into sawdust, and the sawdust was fermented to prepare a fermentation substrate. A comparison was made between the fermentation substrate and the blank substrate, showing that the *Pantotheca acuminata* strain inoculated into the substrate significantly promoted the growth of *Pantotheca acuminata* mycelium.

Provided herein is a method for treating an aqueous cellulose fibre suspension comprising at least one microorganism in a storage tank or a storage tower of a system manufacturing a fibrous web containing cellulose fibres (such as paper, board, or tissue), the method comprising using a combination of (i) a biocide and (ii) nanobubbles comprising an oxidising gas to treat the aqueous cellulose fibre suspension in the storage tank or storage tower, wherein the storage tank or the storage tower comprises at least one inlet conduit for the aqueous fibre suspension to pass into the storage tank or storage tower, and wherein the method comprises administering the nanobubbles to the system such that the concentration of oxygen in the aqueous cellulose fibre suspension in the at least one inlet conduit is between 1 mg / l and 30 mg / l. Also provided are related methods of monitoring and controlling growth of cellulolytic microorganisms or cellulolytic activity, and of manufacturing a fibrous web product containing cellulose fibres, as well as related uses and kits suitable for use in the methods of the invention.

The present invention concerns a bacterial strain of Fibrobacter succinogenes and a composition thereof, and its use as a probiotic in order to improve the digestive fibrolysis.

The present invention concerns a bacterial strain of Fibrobacter succinogenes and a composition thereof, and its use as a probiotic in order to improve the digestive fibrolysis.

The invention relates to a low-carbon-nitrogen-ratio wastewater treatment method and a reactor, denitrification nitrogen removal treatment is performed by using a biological method, and the method specifically comprises the following steps: 1, an organic carbon source can be effectively and slowly released after agricultural waste corncob is pretreated, and two fillers are added into the reactor, so that the organic carbon source can be effectively and slowly released; wherein the filler 1 is a mixture of pretreated corncobs and zeolite, and the filler 2 is a mixture of pretreated corncobs, zeolite and cellulose decomposition bacteria. Two fillers are added into the reactor, so that the biological denitrification efficiency can be effectively improved, and the COD concentration in the discharged water body reaches the standard. Through cooperation of two factors including hydraulic retention time control and arrangement of two fillers in the reactor, ideal denitrification efficiency is achieved, meanwhile, the COD concentration in discharged water is well controlled not to exceed the standard, the COD concentration of effluent is relatively stable, and the COD concentration of the effluent can be further well controlled by controlling the use amount of the reacted fillers in the later period.

The invention relates to processes of producing a fermentation product, comprising liquefying a starch containing material with an alpha-amylase; pre-saccharifying and / or saccharifying and fermenting using a fermentation organism in the presence of a carbohydrate source generating enzyme and a cellulolytic composition The invention also relates to methods of dewatering whole stillage.

The present invention relates to a new Trichoderma strain comprising an additional genetic modification to enable the production of an enzymatic cocktail with enhanced cellulolytic activity, allowing an increase in glucose release and in the saccharification efficiency of plant biomasses.

The invention relates to a double-reflux sectional fermentation process and system, and the fermentation process comprises the following steps: mixing organic wastes with reflux biogas slurry to obtain a raw material, carrying out hydrolytic acidification on the raw material to obtain a first material, controlling the reaction temperature of hydrolytic acidification at 50-55 DEG C, and controlling the first material in a transition section; mixing and reacting the first material with reflux biogas produced by a subsequent anaerobic fermentation section at the reaction temperature of 45-50 DEG C to obtain a second material, and feeding the second material into the anaerobic fermentation section; and fermenting the second material at 40-45 DEG C to produce biogas slurry and biogas. According to the method, different temperatures are set at different stages of the fermentation process, the efficiency of each reaction stage is greatly improved, and in the hydrolysis stage, the high temperature of 50-55 DEG C is combined with microorganisms provided by biogas slurry backflow, so that the cellulose decomposition rate is improved, and the hydrolysis period is shortened; in the anaerobic fermentation section, the optimum activity of methanogens can be maintained at the temperature of 40-45 DEG C, the methane yield is improved, and the methane purity in the biogas is improved.

The invention relates to the technical field of edible fungus cultivation, and discloses a rapid and efficient pleurotus geesteranus strain breeding method, which comprises the following steps: catalyzing a time sequence regulation molecule (component C) to oxidize in a liquid culture medium by utilizing the catalysis priority of laccase to different substrates, and activating a cellulose decomposition enzyme by a product to decompose a slow-release carbon source; then a gel skeleton (component A) is catalyzed to react with a cross-linking agent (component B), and three-dimensional porous hydrogel for uniformly fixing hyphae is formed in situ, so that uniform fixation of the hyphae and synchronous release of nutrients are realized, and the breeding period is shortened. In addition, according to the method, a specific degrading enzyme which is not activated under the initial pH is preset in a culture medium, and after culture is completed, the enzyme can be activated by injecting an activating agent to adjust the pH, so that the solid gel strain is converted into liquid hypha pulp. According to the invention, the strain is liquefied as required, the inoculation operation is simplified, the whole process is carried out in a closed manner, and the pollution risk is reduced.

The invention relates to processes of producing a fermentation product, comprising liquefying a starch containing material with an alpha-amylase; pre-saccharifying and / or saccharifying and fermenting using a fermentation organism in the presence of a carbohydrate source generating enzyme and a cellulolytic composition The invention also relates to methods of dewatering whole stillage.