12 results about "Cellulose breakdown" patented technology

The invention discloses a livestock and poultry breeding feed additive capable of reducing cost and improving effect. The livestock and poultry breeding feed additive is prepared from the following raw materials through compound fermentation: probiotic components: bacillus subtilis, clostridium butyricum and enterococcus; the enzyme preparation components comprise cellulose decomposition enzyme and xylanase; a fermentation medium: brown sugar and water; the traditional Chinese medicine auxiliary materials comprise wormwood, health sand and andrographis paniculata. The preparation method of the cost-reducing and effect-improving livestock and poultry breeding feed additive comprises the following steps of bacterium expanding culture, secondary fermentation and product treatment. The invention belongs to the technical field of livestock and poultry breeding, and particularly provides a livestock and poultry breeding feed additive and a preparation method thereof, and the livestock and poultry breeding feed additive can improve the intestinal microecological balance of livestock and poultry, improve the digestion and utilization rate of feed, enhance the immunity of livestock and poultry and reduce the breeding cost through scientific matching of probiotics, enzyme preparations, traditional Chinese medicine components and nutritional auxiliary materials.

The application discloses a method for producing feed by using Gramineae plant waste, and comprises the following steps: S1, raw material pretreatment: after the Gramineae plant waste is dedusted, cut or crushed, water immersion and ammoniation treatment are carried out, so that the water content reaches 30-60%, and the ammonia addition amount is 3-5% of the dry weight; S2, gas phase blasting: the pretreated material is subjected to gas phase blasting, and the medium is selected from water vapor or non-reactive compressed gas; S3, microbial agent fermentation: a compound microbial agent containing at least two of lactic acid bacteria, yeast bacteria, bacillus and cellulose-decomposing bacteria is added to the blasted material, the water content is adjusted to 60-70%, and sealed fermentation is carried out for 3-7 days; S4, granulation: the fermented material is dried to have a water content of 12-18%, other raw materials are mixed after crushing, and the feed product is obtained through steam conditioning and pressing granulation. Through the cooperation of multiple processes, the method can significantly destroy the structure of the straw, improve the crude protein content and the feeding value, and the product is convenient to store and transport.

本发明公开了一种低温条件下兼具玉米与大豆秸秆降解能力的复合菌剂及其制备方法应用。所述的复合菌剂由保藏编号为CGMCC No.42446的木霉菌（Trichoderma sp.）FS5以及保藏编号为CGMCC No.37567的铜绿假单胞菌（Pseudomonas chlororaphis subsp.）BNT4组成。其中木霉菌FS5能将纤维素分解成较短的纤维寡糖或纤维二糖，铜绿假单胞菌BNT4则可以利用这些中间产物作为碳源，从而避免了这些产物积累对木霉菌FS5产生反馈抑制，间接促进了整个降解过程的效率，从而形成一套高效率的秸秆降解“流水线”。本发明提供的复合菌剂为东北地区主粮作物秸秆的绿色有效处理提供了新的解决方案，对于促进土壤固碳固氮，提高土地肥力及实现农业可持续发展具有重要意义。

The invention discloses a method for efficiently removing heavy metal ions in livestock and poultry manure, which adopts an eluting agent to form a stable chelate with the heavy metal ions through a coordination effect, realizes efficient total removal of the chelate from a manure solid phase, and breaks through the limitation that only the bioavailability can be reduced by a traditional passivation technology. The manure solid phase is subjected to neutral adjustment and then mixed with straw, a compound microbial agent composed of cellulose decomposition bacteria, nitrogen-fixing bacteria and phosphate solubilizing bacteria is inoculated, aerobic fermentation is carried out, and the high-quality organic fertilizer is prepared; leacheate containing heavy metal is subjected to electric-PRB combined treatment, the heavy metal is promoted to be enriched on a PRB material by utilizing electroosmotic flow and electrophoretic migration, and then phosphorus resources are recovered by a struvite crystallization process. According to the method, the removal rate of copper in the livestock and poultry manure is 96.2%, the removal rate of zinc in the livestock and poultry manure is 91.3%, the removal rate of cadmium in the livestock and poultry manure is 98.0%, the agricultural-grade organic fertilizer and the phosphorus recovery liquid are obtained, the process is flexible, the cost is low, industrialization popularization is easy, and the method is suitable for manure treatment of different livestock and poultry types and pollution levels.

The invention discloses a ruminant overwintering concentrate supplement feed and a production method thereof. The ruminant overwintering concentrate supplement feed comprises the following components in parts by weight: 60-90 parts of a basic raw material, 5-15 parts of a cold-resistant stress additive, 1-5 parts of a probiotic compound, 1-5 parts of a flavor improving component and 4-8 parts of a mineral supplement, the probiotic compound comprises lactic acid bacteria, saccharomycetes and cellulose decomposition bacteria; the flavor improving component comprises molasses, vanilla powder, an acidifying agent and citric acid; the mineral supplement comprises calcium carbonate, calcium hydrophosphate, sodium chloride and compound trace elements, wherein the compound trace elements comprise iron, zinc, selenium and manganese. The basic raw materials comprise energy raw materials and protein raw materials, the ratio of the energy raw materials to the protein raw materials is 2: 1, and the energy raw materials are selected from at least two of corn, barley, wheat, wheat bran and rice bran; the protein raw materials are selected from at least two of soybean meal, cottonseed meal, rapeseed meal and peanut meal. The invention relates to the technical field of feed processing, and particularly provides a ruminant overwintering concentrate supplement feed and a production method thereof.

The present invention relates to processes for producing fermentation products from starch-containing material, wherein an alpha-amylase and optionally a thermostable protease, pullulanase and / or glucoamylase are present and / or added during liquefaction, wherein a cellulolytic composition is present and / or added during fermentation or simultaneous saccharification and fermentation. The invention also relates to a composition suitable for use in a process of the invention.

This invention relates to the field of microbial technology, specifically disclosing a *Pantotheca acuminata* strain and its applications. A *Pantotheca acuminata* strain DSX-01 is provided. Through purification and isolation methods, a strain with good cellulose-decomposing ability was obtained, identified as *Pantotheca acuminata* DSX-01. Experiments detecting its cellulose-decomposing enzyme activity showed that *Pantotheca acuminata* DSX-01 has good cellulose-decomposing ability. It was deposited on March 6, 2025, at the China Center for Type Culture Collection (CCTCC), address: Luojia Mountain, Bayi Road, Wuchang District, Wuhan City, Hubei Province; accession number: CCTCC M 2025390. The *Pantotheca acuminata* DSX-01 strain was inoculated into a liquid culture medium to obtain a liquid strain. The *Pantotheca acuminata* strain or its fermentation broth was then inoculated into sawdust, and the sawdust was fermented to prepare a fermentation substrate. A comparison was made between the fermentation substrate and the blank substrate, showing that the *Pantotheca acuminata* strain inoculated into the substrate significantly promoted the growth of *Pantotheca acuminata* mycelium.

The present invention concerns a bacterial strain of Fibrobacter succinogenes and a composition thereof, and its use as a probiotic in order to improve the digestive fibrolysis.

The invention relates to a low-carbon-nitrogen-ratio wastewater treatment method and a reactor, denitrification nitrogen removal treatment is performed by using a biological method, and the method specifically comprises the following steps: 1, an organic carbon source can be effectively and slowly released after agricultural waste corncob is pretreated, and two fillers are added into the reactor, so that the organic carbon source can be effectively and slowly released; wherein the filler 1 is a mixture of pretreated corncobs and zeolite, and the filler 2 is a mixture of pretreated corncobs, zeolite and cellulose decomposition bacteria. Two fillers are added into the reactor, so that the biological denitrification efficiency can be effectively improved, and the COD concentration in the discharged water body reaches the standard. Through cooperation of two factors including hydraulic retention time control and arrangement of two fillers in the reactor, ideal denitrification efficiency is achieved, meanwhile, the COD concentration in discharged water is well controlled not to exceed the standard, the COD concentration of effluent is relatively stable, and the COD concentration of the effluent can be further well controlled by controlling the use amount of the reacted fillers in the later period.

The invention relates to processes of producing a fermentation product, comprising liquefying a starch containing material with an alpha-amylase; pre-saccharifying and / or saccharifying and fermenting using a fermentation organism in the presence of a carbohydrate source generating enzyme and a cellulolytic composition The invention also relates to methods of dewatering whole stillage.

The application discloses a method for pretreating Sargassum biomass by ultrasonic waves combined with sodium sulfite, which uses ultrasonic waves combined with sodium sulfite solution to pretreat Sargassum biomass raw materials; when the ultrasonic waves propagate in the biomass, high-intensity acoustic vibrations are generated, which can cause collisions and friction between the biomass particles, thereby breaking the cell wall and cellulose structure, and being beneficial to the sodium sulfite group to attack the aliphatic side chain of lignin and replace the hydroxyl functional group. The pretreatment of the biomass by the sodium sulfite can remove the lignin and hemicellulose in the Sargassum biomass, and at the same time, make the biomass structure become more porous and loose, reduce the mechanical strength, and increase the accessibility of cellulase. High-concentration fermentable sugar is obtained through the hydrolysis of cellulose-decomposing enzymes. In addition, a buffer solution is selected for the enzymolysis process, because it has better biocompatibility, reduces the potential toxicity of the system to the enzyme, and at the same time, as an organic acid, is easy to biodegrade and has little influence on the environment.

The present invention relates to the fields of fibers and uses thereof such as for producing fiber webs, such as paper, board or tissue. Specifically, the invention relates to a method of monitoring and controlling cellulolytic activity in an aqueous cellulose fiber suspension or process water for a production method of a fibrous web containing cellulose fibers. Also, the present invention relates to a method of manufacturing a fibrous web, such as a paper, board, tissue or the like, and use of a biocide for controlling cellulolytic activity in an aqueous cellulose fiber suspension or in process water e.g. for a production method of a fibrous web containing cellulose fibers. Still, the present invention relates to a fibrous web, such as a paper, board, tissue or the like, an aqueous cellulose fiber suspension or process water for a production method of a fibrous web containing cellulose fibers, and a system for controlling cellulolytic activity in an aqueous fiber suspension or in process water.