43 results about "Cellulose breakdown" patented technology

The invention relates to the field of agricultural soil improvement and ecological fertilization, and discloses an ecological fertilization method for improving the organic matter content of soil in a dry-hot valley region, and the method comprises the following steps: soil matrix improvement: improving the porosity and water binding capacity of the soil by adding charcoal and humic acid particles; the functional microbial preparation is put, and the activity of soil microorganisms is activated through the combined effect of cellulose decomposition bacteria, stabilization promoting flora and water retention flora; green manure crop planting and covering management: planting sweet clover and other leguminous crops to provide a carbon source and a nitrogen source for soil, and meanwhile, utilizing straw covering to inhibit water evaporation; turning over and stabilizing the green manure crops, namely turning over and stabilizing the organic matters in a blooming period of the green manure crops and combining with a stabilization promoting bacterial liquid and an adsorptive carrier; dynamic balance is maintained, and dynamic balance of decomposition, conversion and accumulation of organic matter is achieved step by step through multi-cycle planting and turning and pressing. The organic matter content and fertility of the soil can be improved, and the water-retaining property and microbial ecosystem of the soil are improved.

The invention belongs to the technical field of waste resource utilization, and provides a method for enhancing anaerobic digestion to produce methane. The invention provides a research and development concept of dynamically regulating and controlling nano bubble water injection in stages. The core breakthrough points of the research and development concept comprise: 1) time sequence optimization; based on the pH value of an anaerobic digestion system, H2-NBW or O2-NBW is injected in stages, and the anaerobic digestion hydrolytic acidification stage and the methane production stage are regulated in a targeted manner. O2-NBW is injected in the hydrolytic acidification stage to promote decomposition of protein and cellulose in a substrate, H2-NBW enriched hydrogen nutrition type methanogenesis is switched in the methanogenesis stage, and a hydrogen nutrition type methanogenesis way is enhanced. And (2) when the pH is more than or equal to 6.5 and less than or equal to 7.5 in a neutral safe region, dynamically adjusting the gas type of the nano bubble water by combining the ORP of the anaerobic digestion system, thereby realizing the accurate gradient control of the oxidation reduction potential and improving the methane yield.

The invention discloses streptomyces bulbus as well as a multifunctional fungicide and application thereof. According to the invention, firstly, a strain of Streptomyces tauricus zhanshen-LZ-12 is separated and screened from straw returning soil, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.34456. The invention also discloses a preparation method of the Streptomyces tauricus zhanshen-LZ-12. The strain has the functions of degrading cellulose, decomposing protein, fixing nitrogen, resisting salt, degrading phenolic acid autotoxic substances (benzoic acid, p-hydroxybenzoic acid and cinnamic acid) and the like. Bacterial powder obtained after fermentation of the bacterial strain is uniformly mixed with bacillus bacterial powder to obtain the multifunctional bacterial agent, and when straw returning or composting is carried out, the multifunctional bacterial agent is adopted, straw decomposition can be accelerated, soil ecology can be improved, crop growth can be promoted, and the stress resistance of crops can be enhanced.

The invention discloses a garden branch decomposition inoculant and a preparation method thereof, and relates to the technical field of garden waste treatment.The garden branch decomposition inoculant is composed of microbial strains, organic matter, inorganic nutrients and adjuvants.The garden branch decomposition inoculant shows excellent performance advantages through unique component combination, and the preparation method is simple. A plurality of microbial strains have a synergistic effect, the bacillus subtilis firstly decomposes protein and starch, the bacillus licheniformis inhibits harmful microorganisms and assists cellulose decomposition, and the sporotrichum thermophilus focuses on overcoming cellulose and lignin, so that the decomposition rate of the branches is greatly increased, and the decomposition rate of the branches is increased. Organic substances and inorganic nutrients provide sufficient and balanced nutrient sources for microorganisms and promote vigorous growth and reproduction of the microorganisms, enzyme and vitamin B are added to further enhance the activity of the microorganisms and accelerate the decomposition process, and after the decomposition agent is applied to soil, the soil structure can be remarkably improved, the soil porosity can be increased, and the soil quality can be improved. The air permeability of the soil is better.

The invention belongs to the technical field of fermentation, and particularly relates to a method for preparing an enzyme through multiple fermentation of citrus fruits and the enzyme. The invention relates to a method for preparing enzymes through multiple fermentation of citrus fruits. The method comprises the following steps: S1, pretreatment of raw materials; s2, performing multiple fermentation; and S3, performing post-treatment on the enzyme. According to the present invention, through the complex enzyme synergistic pretreatment, the multi-strain compounding three-stage gradient fermentation and the low-temperature membrane concentration technology, the active components such as vitamin C, flavonoid and the like in the prepared enzyme are efficiently released and most of the active components are retained, the cellulose decomposition rate and the viable count of probiotics are significantly increased, the sensory evaluation is high, and the shelf life is long. The invention provides an innovative solution for industrial production of the citrus enzyme.

The invention discloses quick-acting inflammation-diminishing hemorrhoid cream and a preparation method thereof. Organic compost comprises the following components in percentage by mass: 10-20% of bean cakes, 8-15% of peanut cakes, 5-15% of wheat bran, 20-30% of leaves or green grass, 25-35% of dry cattle and sheep manure, 3-8% of brown sugar and 3-8% of a compound microbial agent containing cellulose decomposition bacteria and lactic acid bacteria. The preparation method comprises the following steps: mixing the raw materials, adjusting the water content to 50-60%, fermenting in a closed environment at 20-35 DEG C for 7-10 days, and periodically turning in the period. Carbon-nitrogen balance is optimized through the specific proportion of the plant source raw materials and the animal waste, the low-temperature anaerobic fermentation process is combined, the fermentation period is remarkably shortened, the humus conversion efficiency is improved, and the obtained compost is free of chemical additives and suitable for green agricultural production.

The invention relates to the technical field of soil improvement, in particular to a complex microbial inoculant suitable for straw organic fertilizer fermentation and a method for improving and fertilizing soil through the complex microbial inoculant. The complex microbial inoculant is formed by compounding a functional microbial inoculant and a carrier according to the mass ratio of 1: (3-5); the functional microbial agent is prepared by mixing lignin degrading bacteria, cellulose decomposing bacteria and functional auxiliary bacteria; all the components of the complex microbial inoculant have a synergistic effect, the fermentation function of the complex microbial inoculant can be greatly improved, the lignin degrading bacteria specifically break ether bonds (-O-) and carbon-carbon bonds (C-C) in a lignin three-dimensional network structure by secreting laccase, manganese peroxidase and the like, lignin is degraded into low-molecular aromatic compounds, and the low-molecular aromatic compounds can be used for degrading the lignin into low-molecular aromatic compounds. Physical wrapping on cellulose is removed, so that a straw fiber structure is converted into a loose structure which is easy to decompose from a lignin-cellulose complex.

The invention discloses a livestock and poultry breeding feed additive capable of reducing cost and improving effect. The livestock and poultry breeding feed additive is prepared from the following raw materials through compound fermentation: probiotic components: bacillus subtilis, clostridium butyricum and enterococcus; the enzyme preparation components comprise cellulose decomposition enzyme and xylanase; a fermentation medium: brown sugar and water; the traditional Chinese medicine auxiliary materials comprise wormwood, health sand and andrographis paniculata. The preparation method of the cost-reducing and effect-improving livestock and poultry breeding feed additive comprises the following steps of bacterium expanding culture, secondary fermentation and product treatment. The invention belongs to the technical field of livestock and poultry breeding, and particularly provides a livestock and poultry breeding feed additive and a preparation method thereof, and the livestock and poultry breeding feed additive can improve the intestinal microecological balance of livestock and poultry, improve the digestion and utilization rate of feed, enhance the immunity of livestock and poultry and reduce the breeding cost through scientific matching of probiotics, enzyme preparations, traditional Chinese medicine components and nutritional auxiliary materials.

The invention discloses a returning-to-field fertilizer based on wheat straw decomposition and a preparation method and application thereof, and belongs to the technical field of organic fertilizers, and the preparation method comprises the following steps: stirring and mixing a sodium alginate solution and a cellulose solution in a reaction kettle, adding protein-coated composite charcoal and a straw decomposition agent, stirring and mixing, and spray-drying to obtain the returning-to-field fertilizer based on wheat straw decomposition. The returning fertilizer based on wheat straw decomposition is obtained; the nitrogen fertilizer slow-release fertilizer is prepared, the urea is loaded in a pore structure formed by the porous biochar and the MOF, and the slow-release performance of the urea is improved through protein coating, so that the nitrogen fertilizer absorption efficiency of crops is improved, the slow-release fertilizer is prepared by blending the straw-decomposing inoculant and the cellulose gel, and the nitrogen fertilizer slow-release effect is improved. The production of cellulolytic enzyme in microorganisms in the straw decomposition agent is improved, and the breeding speed of the microorganisms is improved through the protein, so that the decomposition rate of the straws is improved.

This disclosure relates to a combination, including a composition, of cellulolytic microorganisms, wherein each cellulolytic microorganism produces a glycosylase, said combination comprising: a first cellulolytic microorganism that produces a cellulase classifiable by EC 3.2.1.4 or EC 3.2.1.91; and a second cellulolytic microorganism that produces a cellulase classifiable by EC 3.2.1.4, EC 3.2.1.91, EC 3.2.1.21, EC 3.2.1.52, EC 3.2.1.74 or EC 3.2.1.86. Also disclosed are related methods including to produce a simple carbohydrate from cell wall cellulosic material, and a ruptured cell wall, and to retrieve intracellular materials of the ruptured cell such as lipid or protein.

The invention belongs to the technical field of strigose hydrangea juvenile leaf active substance extraction, and provides a strigose hydrangea juvenile leaf active substance extraction method based on efficient cellulose decomposition bacteria, which comprises the following steps: collecting a soil sample, carrying out microorganism enrichment culture by taking strigose hydrangea juvenile leaf as a carbon source, separating and purifying a single strain, screening cellulose degradation dominant bacteria by adopting a Congo red staining method, and carrying out high-efficiency cellulose decomposition on the dominant bacteria. Then secondary screening is carried out through CMC enzyme activity and beta-glucosidase activity; with a water extraction method as a contrast, screening strains with the best effect of improving the active substances of the strigose hydrangea juvenile leaves; the influence of the inoculum size, the fermentation time and the material-liquid ratio on the extraction of the active substances is investigated through a single-factor experiment; on the basis of the constitution of the cell wall of the sweet tea, cellulose decomposition bacteria with efficient enzyme activity are screened and act on the sweet tea to damage the cell wall structure of the sweet tea, so that the release of active substances in the sweet tea is improved, the extraction rate of sweet tea flavone and sweet tea polyphenol is improved, and a green biological pretreatment technology is formed.

The application discloses a method for producing feed by using Gramineae plant waste, and comprises the following steps: S1, raw material pretreatment: after the Gramineae plant waste is dedusted, cut or crushed, water immersion and ammoniation treatment are carried out, so that the water content reaches 30-60%, and the ammonia addition amount is 3-5% of the dry weight; S2, gas phase blasting: the pretreated material is subjected to gas phase blasting, and the medium is selected from water vapor or non-reactive compressed gas; S3, microbial agent fermentation: a compound microbial agent containing at least two of lactic acid bacteria, yeast bacteria, bacillus and cellulose-decomposing bacteria is added to the blasted material, the water content is adjusted to 60-70%, and sealed fermentation is carried out for 3-7 days; S4, granulation: the fermented material is dried to have a water content of 12-18%, other raw materials are mixed after crushing, and the feed product is obtained through steam conditioning and pressing granulation. Through the cooperation of multiple processes, the method can significantly destroy the structure of the straw, improve the crude protein content and the feeding value, and the product is convenient to store and transport.

The invention relates to the technical field of biomass energy clean conversion, in particular to a power generation system and method based on biomass enzyme catalytic gasification coupling waste recycling, and the system comprises a low-temperature catalytic gasification module, an efficient power generation module and a full-amount recycling module. The method comprises the following steps: catalyzing cellulose and hemicellulose in straws to be decomposed into glucose and xylose in a low-temperature environment and under a micro-positive pressure condition by utilizing a compound preparation consisting of cellulase, ligninase and methanogens, and carrying out anaerobic fermentation to generate methane-containing biogas; biogas is subjected to desulfurization and dehydration pretreatment and then directly generates power through a proton exchange membrane fuel cell, gasification residues are synchronously and completely converted into compound fertilizer through the processes of neutralization, filter pressing, granulation and the like, the comprehensive utilization rate of energy is increased by 50% compared with that of traditional incineration power generation, and a single electric energy output mode is changed.

The present invention relates to a process for producing cellulolytic and / or hemicellulolytic enzymes by a cellulolytic and / or hemicellulolytic microorganism, said process comprising at least:a) a phase of growing the microorganism in the presence of at least one carbon-based substrate, followed byb) a phase of producing the enzymes in the presence of at least one inducing substrate,and also:c) a phase of preparing a carbon-based substrate comprising glucose and / or fructose, which carbon-based substrate is used in either and / or both of the growth phase a) and production phase b), said preparation phase comprising a step c1) of hydrolysis in an acidic aqueous medium, of sucrose to glucose and fructose.

The present invention relates to a method for recycling a cellulose-containing fiber product, and an object of the present invention is to provide a recycling method capable of regenerating a cellulose-based fiber having sufficient strength even in a cellulose material which is difficult to be recycled, such as a material deteriorated due to repeated use, a material having a reduced cellulose polymerization degree due to a decrosslinking agent treatment, a defunctionalizing agent treatment, or a decoloring treatment of a fiber product, or a material having a low polymerization degree of original cellulose itself, such as rayon or lyocell.SOLUTION: The method for recycling a fiber product containing cellulose as a main component includes at least a decomposition step of decomposing cellulose into sugar and a bacterial cellulose production step of producing bacterial cellulose derived from the fiber product using the sugar obtained in the decomposition step as a material.SELECTED DRAWING: None

The invention relates to the technical field of black fungus production, and discloses a black fungus production process, which comprises the following steps: S1, selecting raw materials of a black fungus bag, the raw materials comprising sawdust, bran, soybean meal, gypsum, light calcium carbonate and a pH regulator; the formula comprises the following components in parts by weight: 82-88% of wood chips; 10%-14% of bran; 2%-4% of soybean meal; 0.4%-0.6% of gypsum; according to the black fungus culture medium, the fermented wood chips are used for preparing the culture medium, in the fermentation process, part of macromolecular cellulose and hemicellulose are decomposed into micromolecular carbohydrates, decomposition, absorption and utilization of black fungus hyphae are facilitated, black fungus strains are rapidly planted after inoculation, plant diseases and insect pests can be reduced, the fungus culture time is shortened to 35-40 days, and the yield of the black fungus strains is increased. According to the method, the fermented wood chips are used for cultivation, the black fungus emergence is fast, the black fungus buds are neat, the black fungus mature period is consistent, the method is suitable for one-time harvesting of full-automatic black fungus harvesting, the obtained yield is the same as that of traditional cultivation, only 35-40 days are needed from bag arrangement to harvesting, and the labor production efficiency is improved.

本发明公开了一种低温条件下兼具玉米与大豆秸秆降解能力的复合菌剂及其制备方法应用。所述的复合菌剂由保藏编号为CGMCC No.42446的木霉菌（Trichoderma sp.）FS5以及保藏编号为CGMCC No.37567的铜绿假单胞菌（Pseudomonas chlororaphis subsp.）BNT4组成。其中木霉菌FS5能将纤维素分解成较短的纤维寡糖或纤维二糖，铜绿假单胞菌BNT4则可以利用这些中间产物作为碳源，从而避免了这些产物积累对木霉菌FS5产生反馈抑制，间接促进了整个降解过程的效率，从而形成一套高效率的秸秆降解“流水线”。本发明提供的复合菌剂为东北地区主粮作物秸秆的绿色有效处理提供了新的解决方案，对于促进土壤固碳固氮，提高土地肥力及实现农业可持续发展具有重要意义。

Provided is a method for recycling fiber products containing cellulose, whereby even cellulose materials that are difficult to recycle, such as materials deteriorated by repeated use, materials having a reduced degree of cellulose polymerization due to decrosslinking agent treatment, defunctionalization agent treatment, decolorization treatment, or repeated recycling of fiber products, and materials having a low degree of polymerization of original cellulose such as Rayon or Lyocell, can be regenerated into cellulosic fibers having sufficient strength.　A method for recycling fiber products containing cellulose as a main component comprises at least: a decomposition step for decomposing cellulose into sugar; and a bacterial cellulose generation step for generating fiber product-derived bacterial cellulose by using the sugar obtained by the decomposition step as a material.

The invention discloses a method for efficiently removing heavy metal ions in livestock and poultry manure, which adopts an eluting agent to form a stable chelate with the heavy metal ions through a coordination effect, realizes efficient total removal of the chelate from a manure solid phase, and breaks through the limitation that only the bioavailability can be reduced by a traditional passivation technology. The manure solid phase is subjected to neutral adjustment and then mixed with straw, a compound microbial agent composed of cellulose decomposition bacteria, nitrogen-fixing bacteria and phosphate solubilizing bacteria is inoculated, aerobic fermentation is carried out, and the high-quality organic fertilizer is prepared; leacheate containing heavy metal is subjected to electric-PRB combined treatment, the heavy metal is promoted to be enriched on a PRB material by utilizing electroosmotic flow and electrophoretic migration, and then phosphorus resources are recovered by a struvite crystallization process. According to the method, the removal rate of copper in the livestock and poultry manure is 96.2%, the removal rate of zinc in the livestock and poultry manure is 91.3%, the removal rate of cadmium in the livestock and poultry manure is 98.0%, the agricultural-grade organic fertilizer and the phosphorus recovery liquid are obtained, the process is flexible, the cost is low, industrialization popularization is easy, and the method is suitable for manure treatment of different livestock and poultry types and pollution levels.