Method for preparing isosteviol and sophorose at the same time

A technology for isosteviol and steviol glycosides is applied in the field of simultaneous preparation of two high value-added products, isosteviol and sophorose, which can solve the problems of high separation cost and high purity requirements, and achieves reduction in production cost, improvement in reaction speed, and improvement in The effect of raw material utilization

Active Publication Date: 2009-08-05
巴州正达绿源生物科技有限公司
View PDF1 Cites 30 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0023] In existing reports, a certain amount of sophorose can be hydrolyzed with a single component of high-purity stevioside (SS>98%) separated from RA, and its yield is 25% (Kusakabe, I., Kusama, S. , Murakami, K.Agricultural and biologicalchemistry, 1987,51,2255-2256.), other ingredients in the raw material are not utilized, and this method requires very high purity of the raw material (SS purity>98%)
Therefore, the cost of separating SS and RA in the early stage is very high. Steviol glycosides only use SS in raw materials for hydrolysis, and SS only uses 1 / 4 of sophorose, so further improvement is needed

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing isosteviol and sophorose at the same time
  • Method for preparing isosteviol and sophorose at the same time
  • Method for preparing isosteviol and sophorose at the same time

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Prepare steviol glycoside mixture (SS70%, RA20%, RC and other steviol glycoside ingredients 10%, total steviol glycoside ingredients greater than 90%) aqueous solution concentration 10% (W / W), add concentrated hydrochloric acid to make the concentration 0.1N, normal pressure Under the temperature of 110°C, hydrolyzed for 100min, the pure product of isosteviol was obtained through conventional separation and purification. The HPLC and infrared spectra of isosteviol are respectively shown in figure 1 , figure 2 The hydrolyzate obtains the sophorose product through steps such as conventional separation and purification, and the thin-layer chromatography (TLC) of sophorose sees Figure 4 , Glucose is separated and utilized separately.

Embodiment 2

[0052] Prepare an aqueous solution of stevioside mixture to make the concentration 0.1% (W / W), add concentrated hydrochloric acid to make the concentration 0.01N, mix well, heat and hydrolyze at 150°C for 60 seconds (s), and obtain pure isosteviol through conventional separation and purification. product; the filtrate was separated and purified to obtain sophorose.

Embodiment 3

[0054] Prepare the steviol glycoside mixture aqueous solution to make the concentration 4% (W / W), add 40% hydrobromic acid to make the concentration 0.05N, mix well, shake at 5°C for 24 hours and filter, the filter residue is washed with water, dried and crystallized to obtain different Pure steviol; the filtrate is purified by impurity removal and crystallization to obtain the sophorose component.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
melting pointaaaaaaaaaa
solubility (mass)aaaaaaaaaa
Login to view more

Abstract

The invention discloses a method for hydrolyzing stevioside with rich sources by using acid and synchronously preparing two products with high added values, namely isosteviol and sophorose. An isosteviol product is a medicine with functions of reducing blood sugar, regulating lipid, and the like, the sophorose is a most effective inducer for producing cellulase from strains such as trichoderma, and the like, and the two products have broad application prospect respectively. The method is characterized in that by controlling appropriate conditions, the two high added value products with important industrial application value and medical value, namely the isosteviol and the sophorose are synchronously prepared, and a one-step hydrolysis method is adopted.

Description

technical field [0001] The invention relates to a method for simultaneously preparing two high value-added products of isosteviol and sophorose by using stevioside and its derivatives through acid hydrolysis under normal pressure or pressurized conditions. Background technique [0002] 1. Overview of steviol glycosides [0003] Steviol glycoside, also known as stevioside and stevioside, is a new type of natural sweetener extracted from stevia rebaudiana bertoni in the family Asteraceae. Stevia is a perennial herb of the Asteraceae Stevia family, native to the Amambai Mountains in the northeastern part of Paraguay and Brazil in South America. Wild stevia was used as a sweetener in tea by local residents as early as 400 years ago, and it was not until 1887 that it attracted people's attention. In 1905, it was named Stevia Rebaudiana by the botanist Bertoni. In 1955, artificial nursery cultivation experiments began. In 1964, Paraguay succeeded in field transplantation and do...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07H3/04C07H1/00C07C62/24C07C51/09C07C51/373
Inventor 陈育如刘虎姜中玉刘友芬
Owner 巴州正达绿源生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap