234results about "Disaccharides" patented technology

The invention discloses a method for preparing cellobiose and comprehensively utilizing a by-product of cellobiose. The method is characterized by specifically comprising the following steps: (1) dilution, (2) enzymolysis, (3) flocculation and carbon explosion, (4) decoloration, (5) continuous ion exchange, (6) evaporation and concentration, (7) simulated moving bed chromatographic separation, (8) concentration, (9) finished product preparation and (10) raffinate utilization. The method effectively solves the problems of low preparation purity and high production cost of cellobiose.

The invention discloses a method for enriching whey protein main monomers from cow milk. According to the method, whey protein subjected to membrane separation is taken as a raw material, interaction between main monomers of the whey protein and other milk proteins is weakened by adopting a pH conversion technology and an ultrasonic technology, mineral substances, lactose, bovine serum albumin and residual casein are removed through ion exchange chromatography, the main monomers of the whey protein are enriched, and the high-purity whey protein is obtained. And drying the alpha-lactalbumin and the beta-lactoglobulin to obtain a milk protein product rich in two main monomers of the whey protein, and the total content of the two monomers is up to 85% or above. According to the technology, cow milk serves as a raw material, efficient enrichment of two main monomers of whey protein can be achieved only through membrane separation, pH conversion, ultrasonic assistance and ion exchange chromatography, only pH needs to be adjusted in the separation process, other chemical reagents are not added, and the process is environmentally friendly, simple, easy to implement and suitable for large-scale processing; the prepared milk protein base material rich in the whey protein main monomer can be used as a main material and a base material of infant food, and is high in nutritional value and wide in application prospect.

The invention relates to a preparation method of ginkgo oligosaccharide and a ginkgo oligosaccharide sugar chain plant vaccine, and relates to the field of plant vaccines. The preparation method of the ginkgo oligosaccharide comprises the step that the ginkgo oligosaccharide is extracted from ginkgo testa through an enhanced extraction method, the enhanced extraction method is an extraction method combining an ultrasonic extraction method and a water extraction and alcohol precipitation method, the purity of the oligosaccharide is improved, the structure of the oligosaccharide is not changed, and the cost is reduced. The ginkgo oligosaccharide is used for preparing the plant vaccine, and the vaccine takes the ginkgo oligosaccharide extracted from ginkgo testa as a main component, and has the function of inducing plants to generate stress resistance such as disease resistance, drought resistance, cold resistance and hot air resistance.

The present disclosure describes compositions and methods including liposome or lipid nanoparticles (LNPs) including carbohydrate-based ligands that target the liver, lungs, and / or kidneys. More particularly, the present disclosure relates to compositions and methods including liposome or lipid nanoparticles (LNPs) including carbohydrate-based ligands, such as di-lactobionic acid (LBA) ligand, and a suitable cargo such as nucleic acids and small molecule to target the liver, lungs, and / or kidneys for treatment and / or diagnosis of diseases and conditions.

To provide a binding syrup composition for formulating food products containing allulose and low sugar syrups.SOLUTION: The binding syrup composition overcomes limitations of allulose-containing products including product stickiness and failure to maintain shape and texture of the products. More specifically, the binding syrup compositions described herein contain low sugar syrups with low mono- and di-saccharide content in combination with allulose to impart binding and textural attributes to the allulose-containing food products that are comparable to full sugar products. Food products containing the allulose binding syrup compositions described herein and methods of producing the same are also provided. Beneficially, food products having reduced caloric and sugar content are provided when employing the allulose-containing binding syrup compositions described herein.SELECTED DRAWING: Figure 1

The invention relates to the technical field of trehalose preparation, in particular to high-purity trehalose and a crystallization method and application thereof. The crystallization method comprises the following steps: (1) heating a trehalose solution to 60-65 DEG C, and adding a seed crystal to obtain a seed crystal sugar solution; (2) cooling the seed crystal sugar solution to 25-30 DEG C to obtain a trehalose crystal; (3) heating the trehalose crystals to 60-65 DEG C, and keeping the temperature for 10-20 minutes to obtain a trehalose mixture; and (4) repeating the steps (2)-(3) for 0-2 times, cooling the trehalose mixture to 25-30 DEG C, keeping for 9-10 hours, centrifuging, drying and screening to obtain the high-purity trehalose. The high-purity trehalose prepared by the method disclosed by the invention is high in purity, few in impurities, uniform in trehalose crystal particle, stable in quality and high in recovery rate, and can be widely applied to the fields of food, medicine, daily chemical industry and the like.

The invention provides a continuous crystallization method of block-shaped trehalose crystals, which comprises the following steps: stimulating trehalose syrup to crystallize to form seed syrup, growing the crystal at 60-65 DEG C for 1-2 hours, and then cooling and crystallizing to obtain the block-shaped trehalose crystals, the cooling crystallization is three-stage cooling crystallization and comprises the following steps: cooling to 50-55 DEG C at a cooling rate of 0.5-0.8 DEG C / h, then cooling to 40-43 DEG C at a cooling rate of 1-1.5 DEG C / h, and then cooling to 25-30 DEG C at a cooling rate of 2-2.5 DEG C / h. Compared with a commercially available trehalose product, the purity of the trehalose product is greater than 99.8%, the length-diameter ratio is less than 2: 1, the grain size of 97wt% or more seed crystals is greater than 60 meshes, the filtering time is shortened by 62.2%, the bulk density of the product is improved by 49.3%, the particles are uniform, and when the trehalose product is compounded with other products, the proportioning amount can be accurately mastered, and the quality of the compounded product is improved.

The invention provides a method for preparing lactulose and co-producing lactitol, and belongs to the field of functional sugar. The method comprises the following steps: sequentially carrying out first high-temperature isomerization, concentration and second high-temperature isomerization on a decolored lactose solution to obtain a mixed solution; sequentially carrying out refining and chromatographic separation extraction on the mixed solution to obtain a lactulose solution and raffinate; refining, crystallizing and drying the lactulose solution to obtain crystalline lactulose; refining the raffinate, adjusting the pH value, and carrying out hydrogenation reaction to obtain a lactitol crude liquid; the lactitol crude liquid is subjected to refining and nanofiltration membrane filtration in sequence, and a lactitol solution is obtained; and refining, crystallizing and drying the lactitol solution in sequence to obtain crystallized lactitol. According to the method, the lactose is subjected to two times of high-temperature conversion under the alkaline condition, the conversion rate of the lactulose can reach 55% or above and is far higher than that of an existing lactulose preparation method, the content of the lactose in the converted feed liquid is reduced, and the problem that unreacted lactose is easy to separate out and crystallize in the lactulose preparation process is solved.

The invention relates to the field of xylooligosaccharide production, and discloses a xylooligosaccharide production method, which comprises: raw material pretreatment: removing impurities from a raw material rich in lignocellulose, and crushing; acid liquid is sprayed into the fine powder, mixing and stirring are conducted at the same time, and heating is conducted for a sugar soaking reaction; gradient sugar washing: adding deionized water into the residue sugar mixture to carry out multiple rounds of gradient sugar washing, and separating to obtain a high-concentration sugar solution; electrodialysis acid recovery: carrying out deacidification treatment on the high-concentration sugar solution by using electrodialysis, and recovering the acid solution for reutilization; and continuous separation and preparation of xylooligosaccharide: adjusting the pH value of the sugar solution to 4.0-6.5, separating the xylooligosaccharide by adopting a nanofiltration membrane, and adding xylanase into a nanofiltration membrane concentration tank at the same time, so that the intercepted xylan is continuously degraded into the xylooligosaccharide. According to the method, xylooligosaccharide separation and xylooligosaccharide preparation are combined together, so that xylooligosaccharide obtained by xylanase hydrolysis can penetrate through a nanofiltration membrane in time, xylooligosaccharide is prevented from being continuously hydrolyzed into xylose, and generation of by-products is reduced.

The preparation method comprises the following steps: (1) dissolving agar in a citric acid monohydrate solution, and carrying out acidolysis to obtain an acidolysis solution; centrifuging, filtering, adding beta-agarase AgWH50B, and carrying out enzymolysis for 20-28 hours at the temperature of 30-35 DEG C; boiling, centrifuging and filtering to obtain supernate, namely crude oligosaccharide liquid containing agarotriose; (2) dissolving agar in a phosphoric acid solution, and degrading for 55-65 minutes at the temperature of 97-103 DEG C; centrifuging and filtering to obtain supernate, namely crude oligosaccharide liquid containing agarobiose; (3) dissolving agar in deionized water, adding alpha-agarase A33, and carrying out enzymolysis at 38-42 DEG C for 20-28 hours; and boiling, centrifuging and filtering to obtain supernate, namely crude oligosaccharide liquid containing agarotetraose. The preparation method of the agaro-oligosaccharide is used for preparing agarotriose, agarobiose and agarotetraose, and the product purity is high. The method can provide guidance for subsequent large-scale industrial production of agaro-oligosaccharide.

The invention discloses a method for preparing high-purity brown algae oligosaccharide, and belongs to the field of marine chemical technology. The method for preparing brown algae oligosaccharide of the invention comprises the following steps: after sodium alginate is divided into three times for enzymatic hydrolysis, the enzymatic hydrolysis liquid is ultrafiltered to obtain a brown algae oligosaccharide clear liquid; the brown algae oligosaccharide clear liquid is spray-dried to obtain the brown algae oligosaccharide. In the invention, the sodium alginate has a high degree of degradation, the obtained brown algae oligosaccharide has a high purity, and the production process is safe and efficient.

Aspects of the disclosure relate to oligosaccharide compositions and methods of making the same. Also provided are methods of using oligosaccharide compositions as microbiome metabolic therapies for reducing ammonia levels and for the treatment of hyperammonemia-related diseases (e.g., urea cycle disorders and hepatic encephalopathy).

The invention relates to a method for simultaneously extracting dietary fiber and oligosaccharide from beet pulp, which comprises the following steps: mixing pretreated beet pulp with water, extracting, filtering to obtain a first filter residue and a first supernatant, mixing the first filter residue with alkali, and centrifuging to obtain a second filter residue and a second supernatant; mixing the second filter residue with an acid, and centrifuging to obtain a third filter residue and a third supernatant; mixing the third filter residue with an enzyme for enzymolysis, and centrifuging to obtain a fourth filter residue and a fourth supernate; mixing all the supernatants, performing ultrafiltration concentration to obtain a concentrated solution with the molecular weight of 500-10kDa, and performing freeze drying to obtain oligosaccharide; the concentrated solution with the molecular weight larger than 10 kDa is subjected to ethanol precipitation, and soluble dietary fibers are obtained; and mixing the fourth filter residue with a hydrogen peroxide solution, boiling, and filtering to obtain a precipitate which is the insoluble dietary fiber. According to the method, three substances are simultaneously extracted in one technological process, so that resources are comprehensively utilized, and the extraction efficiency is maximized.

Disclosed is a wood-derived carbohydrate composition, wherein the carbohydrate composition comprises at least 40 weight-% of carbohydrates based on the total dry matter content of the carbohydrate composition, at least 30 weight-% of the carbohydrates are xylose, and at least 50 weight-% of the xylose is oligomeric xylose. Further is disclosed the use of the wood-derived carbohydrate composition.

The present invention provides a neomycin-lactulose cocrystal and a preparation method thereof. The cocrystal is prepared from neomycin and lactulose. The powder X-ray diffraction pattern of the neomycin-lactulose cocrystal has diffraction angles 2θ=12.3°±0.2°, 13.9°±0.2°, 14.9°±0.2°, 16.2°±0.2°, 17.3°±0.2°, 18.1°±0.2°, 18.8° The neomycin-lactulose cocrystal of the present invention has characteristic peaks at 14.5°±0.2°, 19.5°±0.2°, 22.1°±0.2°, 23.2°±0.2°, 24.7°±0.2°, 25.4°±0.2°, 27.4°±0.2°, 28.0°±0.2°, 29.2°±0.2°, 29.8°±0.2°, 31.7°±0.2°, and 33.3°±0.2°. Compared with existing neomycin, the neomycin-lactulose cocrystal of the present invention has more stable physical properties and significantly improved bioavailability under the same conditions.

The invention discloses a Enteromorpha oligosaccharide and its preparation method and application. The Enteromorpha oligosaccharide is prepared by degradation of Enteromorpha polysaccharide, has a degree of polymerization of 2-10, contains rhamnose, glucose, glucuronic acid and xylose, and has a simple preparation method. The present invention verifies for the first time that Enteromorpha oligosaccharide can significantly reduce the fasting blood sugar level of Type 2 diabetic mice, reduce the insulin content and glycosylated serum protein level in serum, improve the blood lipid metabolism ability of Type 2 diabetic mice, improve the glycogen synthesis ability in the liver of mice, improve the liver function abnormality of Type 2 diabetic mice, and Enteromorpha oligosaccharide has high safety, and has good prospects in developing food or medicine with the function of controlling Type 2 diabetes.

This invention discloses an acid-catalyzed controlled degradation and recycling method for waste polyester and polyester-cotton blended fabrics. Using waste polyester or waste polyester-cotton blended fabrics as raw materials, under the action of an acidic catalyst, in a mixed solvent composed of a polar aprotic solvent and water, the method involves heating and degradation, causing the polyester in the waste polyester or polyester-cotton blended fabrics to depolymerize or co-depolymerize, generating organic raw materials. This invention achieves complete depolymerization and recycling of polyester with a relatively low acid dosage, effectively avoiding the corrosion problems of equipment and excessive degradation of cotton fibers caused by existing technologies. It is also more conducive to the generation and recovery of glucose and 5-hydroxymethylfurfural. Furthermore, the separation steps are simple, greatly reducing separation costs, and high-yield recovery of terephthalic acid, glucose, and 5-hydroxymethylfurfural is achieved, realizing high-value recycling and utilization of various components in textiles, reducing waste, and improving resource utilization.

A binding syrup composition for formulating food products containing allulose and low sugar syrups is provided. The binding syrup composition overcomes limitations of allulose-containing products including product stickiness and failure to maintain shape and texture of the products. More particularly, the binding syrup compositions described herein contain low sugar syrups with low mono- and di-saccharide content in combination with allulose to provide binding and textural attributes to the allulose-containing food products that are comparable to full sugar products. Food products containing the allulose binding syrup compositions described herein and methods of making the same are also provided. Beneficially, food products having reduced caloric and sugar content are provided when employing the allulose-containing binding syrup compositions described herein.

The present invention relates to a psicose syrup with improved properties.

The embodiment of the invention discloses a preparation method of a concentrated lactulose solution. The method comprises the following steps: by taking a lactose solution as a raw material and sodium sulfite as a catalyst, carrying out an isomerization reaction in the presence of alkali, and then sequentially carrying out primary crystallization, primary centrifugal separation, deionization treatment, concentration, secondary crystallization, secondary centrifugal separation, filtration and concentration adjustment to prepare the concentrated lactulose solution. According to the method, a raw material recycling mode of'reaction-separation-circulation 'is adopted, lactose which does not participate in an isomerization reaction is recycled by adopting two-time crystallization centrifugal separation operation, and the lactose is put into the isomerization reaction again, so that the recycling of the raw materials is realized. The method effectively avoids the problem of environmental pollution caused by boric acid or borate as a catalyst, simplifies the process, reduces the cost, has high product purity, and is suitable for industrial large-scale production.

The invention belongs to the technical field of biomass conversion and functional food ingredients, and discloses a method for preparing galactooligosaccharide prebiotics from cellulose. The method comprises the following steps: mixing cellulose with a galactose aqueous solution, and drying to obtain galactose pre-impregnated cellulose; impregnating the cellulose pre-impregnated with galactose with an acid catalyst solution, and drying to obtain cellulose impregnated with galactose and an acid catalyst; carrying out solvent-free ball milling treatment on the cellulose impregnated with the galactose and the acid catalyst, so that the cellulose is synchronously subjected to depolymerization and grafting reaction with the galactose to obtain a crude product; and carrying out purification treatment on the crude product, and removing the residual acid catalyst to obtain the galactooligosaccharide prebiotics. According to the galactooligosaccharide prepared by the preparation method disclosed by the invention, the water solubility of the oligosaccharide is greatly improved due to the introduction of a galactose branched chain, the yield of a final product is high, and the prebiotic activity of the final product is equivalent to that of a high-cost enzymic method commercial product and even is better for a specific strain.

The present invention relates to a process for preparing a dried carbohydrate preparation from a water-containing crystalline carbohydrate starting preparation and carbohydrate particles, and to the carbohydrate preparation prepared by the process.

The invention relates to the technical field of saccharide purification, in particular to a high-yield and low-color-value anhydrous beta-lactose preparation method which comprises the following steps: preparing a lactose solution by taking food-grade lactose as a raw material, adding activated carbon for adsorption, then carrying out column treatment to remove nitrogen-containing inorganic salt and organic acid, and finally carrying out film evaporation concentration to separate out beta-lactose crystals. The method comprises the following steps of: adding anhydrous beta-lactose into a reaction kettle, automatically scraping off crystals, and collecting to obtain anhydrous beta-lactose, the yield of the anhydrous beta-lactose is at least 90wt%, and the color value is lower than 25. According to the method, the yield of the beta-lactose can be improved, and the produced beta-lactose is uniform in particle size, normal in color and few in impurities.

The invention discloses a preparation method of curdlan oligosaccharide, which comprises the following steps: mixing curdlan with an L molecular sieve, performing ball milling, adding a solvent for dissolving, performing solid-liquid separation on the L molecular sieve, and drying to obtain the curdlan oligosaccharide, the chemical composition of the L molecular sieve is as follows: 1 to 5 percent of K2O, 1.0 percent of Al2O3, 5 to 10 percent of SiO2 and 1 to 5 percent of H2O, and the L molecular sieve has a straight pore channel. According to the L molecular sieve with the straight pore channels, glucosidic bonds of curdlan can be selectively sheared to prepare curdlan oligosaccharides; therefore, efficient preparation of curdlan oligosaccharide is realized, and the curdlan oligosaccharide can be repeatedly used; the method does not need a large amount of water or other solvents to participate in the reaction, and has the advantages of environmental friendliness, low cost and the like; compared with existing reports, the method has obvious advantages and is more beneficial to industrialization.

The present invention relates to the use of antibodies against Thomsen-Friedenreich (TF) as reagents for in vitro diagnosis of microsatellite instability (MSI). Another aspect of the present invention relates to a method for in vitro diagnosis of MSI, which comprises using a composition of antibodies against TF antigen and measuring the expression of TF antigen in a tissue sample. The present invention also relates to a kit comprising the above-mentioned composition, which is used to detect MSI by implementing the method of the present invention. By sensitively and specifically detecting MSI in patients with gastric cancer, colorectal cancer and cancer of other tissues based on a single marker, the use, composition, method and kit of the present invention can be advantageously used to significantly promote the identification of MSI in clinical situations, thereby being used for diagnosis, prognosis or prediction of response to immunotherapy.

This invention relates to a catalyst for degrading polysaccharides, its preparation method, and its application, belonging to the field of polysaccharide degradation technology. The invention provides a method for preparing a catalyst for degrading polysaccharides, comprising: weighing fumaric acid and hydrogen peroxide, mixing them, and preparing the catalyst. The molar ratio of fumaric acid to hydrogen peroxide is 0.5–2:0.5–2. The invention also provides a catalyst for degrading polysaccharides, obtained by the above preparation method. The catalyst prepared by this invention using fumaric acid and hydrogen peroxide for the degradation of galactomannan can improve the polysaccharide degradation efficiency, resulting in high yields of low molecular weight galactomannan and galactomannan oligosaccharides, thus increasing productivity.

Disclosed herein are strategies for making α-D-glucopyranosyl-β-allulofuranoside (a disaccharide containing glucose and allulose moieties) also referred to herein as SUPERCROSE™. Also disclosed are allulose-glucose mixtures lacking fructose for enhancing flavor of food and beverages.

There is provided a resident-skin-flora-improving agent that is useful in keeping the skin in excellent condition. Specifically, there is provided a resident-skin-flora-improving agent in which sophorose is used. Sophorose is provided with functionality by which it is possible to promote proliferation of helpful bacteria that beneficially affect skin while suppressing growth of bacteria that could adversely affect the skin. The resident-skin-flora-improving agent can suitably be used for the object of maintaining balance of the resident skin flora and keeping the skin in excellent condition.

The invention discloses a preparation technology of astragalus polysaccharide camouflage monosaccharide, and relates to the technical field of biological product production, the preparation technology comprises the following steps: S1, extraction, S2, purification: carrying out purification treatment on astragalus polysaccharide extracted in the step 1, S3, degradation camouflage: dissolving the astragalus polysaccharide purified in the step 2 in a solvent, carrying out degradation treatment on the astragalus polysaccharide by adopting ultrasonic waves, and carrying out purification treatment on the astragalus polysaccharide to obtain the astragalus polysaccharide camouflage monosaccharide. A specific glucan structural unit in a polysaccharide chain is degraded and disguised into a monosaccharide or oligosaccharide structure; and S4, separating to obtain a product. Astragalus polysaccharide is directionally camouflaged as a monosaccharide / oligosaccharide structure, the recognition and uptake efficiency of astragalus polysaccharide by cancer cells is remarkably enhanced, so that the targeting of inducing apoptosis of cancer cells is improved, the structure can effectively activate and train immune cells such as dendritic cells and strengthen anti-tumor immune response, and meanwhile, the product can interfere with a metabolic pathway of cancer cells and reverse the drug resistance of cancer cells, so that the anti-tumor effect is improved. Nutrition supply is blocked to inhibit tumor growth, the production process is green and controllable, and good clinical transformation and industrialization prospects are achieved.