174results about "Disaccharides" patented technology

The invention relates to a preparation method of ginkgo oligosaccharide and a ginkgo oligosaccharide sugar chain plant vaccine, and relates to the field of plant vaccines. The preparation method of the ginkgo oligosaccharide comprises the step that the ginkgo oligosaccharide is extracted from ginkgo testa through an enhanced extraction method, the enhanced extraction method is an extraction method combining an ultrasonic extraction method and a water extraction and alcohol precipitation method, the purity of the oligosaccharide is improved, the structure of the oligosaccharide is not changed, and the cost is reduced. The ginkgo oligosaccharide is used for preparing the plant vaccine, and the vaccine takes the ginkgo oligosaccharide extracted from ginkgo testa as a main component, and has the function of inducing plants to generate stress resistance such as disease resistance, drought resistance, cold resistance and hot air resistance.

The invention provides a continuous crystallization method of block-shaped trehalose crystals, which comprises the following steps: stimulating trehalose syrup to crystallize to form seed syrup, growing the crystal at 60-65 DEG C for 1-2 hours, and then cooling and crystallizing to obtain the block-shaped trehalose crystals, the cooling crystallization is three-stage cooling crystallization and comprises the following steps: cooling to 50-55 DEG C at a cooling rate of 0.5-0.8 DEG C / h, then cooling to 40-43 DEG C at a cooling rate of 1-1.5 DEG C / h, and then cooling to 25-30 DEG C at a cooling rate of 2-2.5 DEG C / h. Compared with a commercially available trehalose product, the purity of the trehalose product is greater than 99.8%, the length-diameter ratio is less than 2: 1, the grain size of 97wt% or more seed crystals is greater than 60 meshes, the filtering time is shortened by 62.2%, the bulk density of the product is improved by 49.3%, the particles are uniform, and when the trehalose product is compounded with other products, the proportioning amount can be accurately mastered, and the quality of the compounded product is improved.

The invention relates to the field of xylooligosaccharide production, and discloses a xylooligosaccharide production method, which comprises: raw material pretreatment: removing impurities from a raw material rich in lignocellulose, and crushing; acid liquid is sprayed into the fine powder, mixing and stirring are conducted at the same time, and heating is conducted for a sugar soaking reaction; gradient sugar washing: adding deionized water into the residue sugar mixture to carry out multiple rounds of gradient sugar washing, and separating to obtain a high-concentration sugar solution; electrodialysis acid recovery: carrying out deacidification treatment on the high-concentration sugar solution by using electrodialysis, and recovering the acid solution for reutilization; and continuous separation and preparation of xylooligosaccharide: adjusting the pH value of the sugar solution to 4.0-6.5, separating the xylooligosaccharide by adopting a nanofiltration membrane, and adding xylanase into a nanofiltration membrane concentration tank at the same time, so that the intercepted xylan is continuously degraded into the xylooligosaccharide. According to the method, xylooligosaccharide separation and xylooligosaccharide preparation are combined together, so that xylooligosaccharide obtained by xylanase hydrolysis can penetrate through a nanofiltration membrane in time, xylooligosaccharide is prevented from being continuously hydrolyzed into xylose, and generation of by-products is reduced.

The preparation method comprises the following steps: (1) dissolving agar in a citric acid monohydrate solution, and carrying out acidolysis to obtain an acidolysis solution; centrifuging, filtering, adding beta-agarase AgWH50B, and carrying out enzymolysis for 20-28 hours at the temperature of 30-35 DEG C; boiling, centrifuging and filtering to obtain supernate, namely crude oligosaccharide liquid containing agarotriose; (2) dissolving agar in a phosphoric acid solution, and degrading for 55-65 minutes at the temperature of 97-103 DEG C; centrifuging and filtering to obtain supernate, namely crude oligosaccharide liquid containing agarobiose; (3) dissolving agar in deionized water, adding alpha-agarase A33, and carrying out enzymolysis at 38-42 DEG C for 20-28 hours; and boiling, centrifuging and filtering to obtain supernate, namely crude oligosaccharide liquid containing agarotetraose. The preparation method of the agaro-oligosaccharide is used for preparing agarotriose, agarobiose and agarotetraose, and the product purity is high. The method can provide guidance for subsequent large-scale industrial production of agaro-oligosaccharide.

Aspects of the disclosure relate to oligosaccharide compositions and methods of making the same. Also provided are methods of using oligosaccharide compositions as microbiome metabolic therapies for reducing ammonia levels and for the treatment of hyperammonemia-related diseases (e.g., urea cycle disorders and hepatic encephalopathy).

The invention relates to a method for simultaneously extracting dietary fiber and oligosaccharide from beet pulp, which comprises the following steps: mixing pretreated beet pulp with water, extracting, filtering to obtain a first filter residue and a first supernatant, mixing the first filter residue with alkali, and centrifuging to obtain a second filter residue and a second supernatant; mixing the second filter residue with an acid, and centrifuging to obtain a third filter residue and a third supernatant; mixing the third filter residue with an enzyme for enzymolysis, and centrifuging to obtain a fourth filter residue and a fourth supernate; mixing all the supernatants, performing ultrafiltration concentration to obtain a concentrated solution with the molecular weight of 500-10kDa, and performing freeze drying to obtain oligosaccharide; the concentrated solution with the molecular weight larger than 10 kDa is subjected to ethanol precipitation, and soluble dietary fibers are obtained; and mixing the fourth filter residue with a hydrogen peroxide solution, boiling, and filtering to obtain a precipitate which is the insoluble dietary fiber. According to the method, three substances are simultaneously extracted in one technological process, so that resources are comprehensively utilized, and the extraction efficiency is maximized.

Disclosed is a wood-derived carbohydrate composition, wherein the carbohydrate composition comprises at least 40 weight-% of carbohydrates based on the total dry matter content of the carbohydrate composition, at least 30 weight-% of the carbohydrates are xylose, and at least 50 weight-% of the xylose is oligomeric xylose. Further is disclosed the use of the wood-derived carbohydrate composition.

The present invention provides a neomycin-lactulose cocrystal and a preparation method thereof. The cocrystal is prepared from neomycin and lactulose. The powder X-ray diffraction pattern of the neomycin-lactulose cocrystal has diffraction angles 2θ=12.3°±0.2°, 13.9°±0.2°, 14.9°±0.2°, 16.2°±0.2°, 17.3°±0.2°, 18.1°±0.2°, 18.8° The neomycin-lactulose cocrystal of the present invention has characteristic peaks at 14.5°±0.2°, 19.5°±0.2°, 22.1°±0.2°, 23.2°±0.2°, 24.7°±0.2°, 25.4°±0.2°, 27.4°±0.2°, 28.0°±0.2°, 29.2°±0.2°, 29.8°±0.2°, 31.7°±0.2°, and 33.3°±0.2°. Compared with existing neomycin, the neomycin-lactulose cocrystal of the present invention has more stable physical properties and significantly improved bioavailability under the same conditions.

This invention discloses an acid-catalyzed controlled degradation and recycling method for waste polyester and polyester-cotton blended fabrics. Using waste polyester or waste polyester-cotton blended fabrics as raw materials, under the action of an acidic catalyst, in a mixed solvent composed of a polar aprotic solvent and water, the method involves heating and degradation, causing the polyester in the waste polyester or polyester-cotton blended fabrics to depolymerize or co-depolymerize, generating organic raw materials. This invention achieves complete depolymerization and recycling of polyester with a relatively low acid dosage, effectively avoiding the corrosion problems of equipment and excessive degradation of cotton fibers caused by existing technologies. It is also more conducive to the generation and recovery of glucose and 5-hydroxymethylfurfural. Furthermore, the separation steps are simple, greatly reducing separation costs, and high-yield recovery of terephthalic acid, glucose, and 5-hydroxymethylfurfural is achieved, realizing high-value recycling and utilization of various components in textiles, reducing waste, and improving resource utilization.

The present invention relates to a psicose syrup with improved properties.

The embodiment of the invention discloses a preparation method of a concentrated lactulose solution. The method comprises the following steps: by taking a lactose solution as a raw material and sodium sulfite as a catalyst, carrying out an isomerization reaction in the presence of alkali, and then sequentially carrying out primary crystallization, primary centrifugal separation, deionization treatment, concentration, secondary crystallization, secondary centrifugal separation, filtration and concentration adjustment to prepare the concentrated lactulose solution. According to the method, a raw material recycling mode of'reaction-separation-circulation 'is adopted, lactose which does not participate in an isomerization reaction is recycled by adopting two-time crystallization centrifugal separation operation, and the lactose is put into the isomerization reaction again, so that the recycling of the raw materials is realized. The method effectively avoids the problem of environmental pollution caused by boric acid or borate as a catalyst, simplifies the process, reduces the cost, has high product purity, and is suitable for industrial large-scale production.

The invention belongs to the technical field of biomass conversion and functional food ingredients, and discloses a method for preparing galactooligosaccharide prebiotics from cellulose. The method comprises the following steps: mixing cellulose with a galactose aqueous solution, and drying to obtain galactose pre-impregnated cellulose; impregnating the cellulose pre-impregnated with galactose with an acid catalyst solution, and drying to obtain cellulose impregnated with galactose and an acid catalyst; carrying out solvent-free ball milling treatment on the cellulose impregnated with the galactose and the acid catalyst, so that the cellulose is synchronously subjected to depolymerization and grafting reaction with the galactose to obtain a crude product; and carrying out purification treatment on the crude product, and removing the residual acid catalyst to obtain the galactooligosaccharide prebiotics. According to the galactooligosaccharide prepared by the preparation method disclosed by the invention, the water solubility of the oligosaccharide is greatly improved due to the introduction of a galactose branched chain, the yield of a final product is high, and the prebiotic activity of the final product is equivalent to that of a high-cost enzymic method commercial product and even is better for a specific strain.

This invention relates to a catalyst for degrading polysaccharides, its preparation method, and its application, belonging to the field of polysaccharide degradation technology. The invention provides a method for preparing a catalyst for degrading polysaccharides, comprising: weighing fumaric acid and hydrogen peroxide, mixing them, and preparing the catalyst. The molar ratio of fumaric acid to hydrogen peroxide is 0.5–2:0.5–2. The invention also provides a catalyst for degrading polysaccharides, obtained by the above preparation method. The catalyst prepared by this invention using fumaric acid and hydrogen peroxide for the degradation of galactomannan can improve the polysaccharide degradation efficiency, resulting in high yields of low molecular weight galactomannan and galactomannan oligosaccharides, thus increasing productivity.

There is provided a resident-skin-flora-improving agent that is useful in keeping the skin in excellent condition. Specifically, there is provided a resident-skin-flora-improving agent in which sophorose is used. Sophorose is provided with functionality by which it is possible to promote proliferation of helpful bacteria that beneficially affect skin while suppressing growth of bacteria that could adversely affect the skin. The resident-skin-flora-improving agent can suitably be used for the object of maintaining balance of the resident skin flora and keeping the skin in excellent condition.

The invention discloses a preparation technology of astragalus polysaccharide camouflage monosaccharide, and relates to the technical field of biological product production, the preparation technology comprises the following steps: S1, extraction, S2, purification: carrying out purification treatment on astragalus polysaccharide extracted in the step 1, S3, degradation camouflage: dissolving the astragalus polysaccharide purified in the step 2 in a solvent, carrying out degradation treatment on the astragalus polysaccharide by adopting ultrasonic waves, and carrying out purification treatment on the astragalus polysaccharide to obtain the astragalus polysaccharide camouflage monosaccharide. A specific glucan structural unit in a polysaccharide chain is degraded and disguised into a monosaccharide or oligosaccharide structure; and S4, separating to obtain a product. Astragalus polysaccharide is directionally camouflaged as a monosaccharide / oligosaccharide structure, the recognition and uptake efficiency of astragalus polysaccharide by cancer cells is remarkably enhanced, so that the targeting of inducing apoptosis of cancer cells is improved, the structure can effectively activate and train immune cells such as dendritic cells and strengthen anti-tumor immune response, and meanwhile, the product can interfere with a metabolic pathway of cancer cells and reverse the drug resistance of cancer cells, so that the anti-tumor effect is improved. Nutrition supply is blocked to inhibit tumor growth, the production process is green and controllable, and good clinical transformation and industrialization prospects are achieved.

The invention discloses a high-efficiency separation method of breast milk oligosaccharide and application of the breast milk oligosaccharide in specific recognition of influenza A virus hemagglutinin protein.The method comprises the steps that a DE-52 weak anion exchange column, a graphite carbon column and Bio-GelP10 column chromatography are combined for use, breast milk oligosaccharide components with low abundance and high molecular weight are efficiently enriched, and the specific recognition of influenza A virus hemagglutinin protein is achieved; after derivatization labeling of a bifunctional reagent 2-amino-N-(2-aminoethyl) benzamide (AEAB), 56 breast milk oligosaccharide monomers with the purity higher than 90% are separated from the isomer level through one-dimensional amide normal phase chromatography and two-dimensional C18 reverse phase circulation chromatography, and a monomer substance library is constructed. AEAB is used as a connecting arm to construct a breast milk oligosaccharide chip, an active monomer DSLNT II which is specifically combined with four subtype influenza A virus hemagglutinin proteins is screened out in a high-throughput manner, and fine structure analysis is completed in combination with electrospray tandem mass spectrometry. According to the method, the large-scale preparation of the low-abundance breast milk oligosaccharide monomer is realized, and a key material basis and theoretical guidance are provided for researching an antiviral mechanism and developing related functional foods and medicines.

To provide a pharmaceutical formulation comprising trehalose for use in treating myopathies, neurodegenerative diseases, or tauopathies associated with abnormal protein aggregation.SOLUTION: A pharmaceutical formulation comprising a therapeutically effective amount of trehalose is provided, wherein the pharmaceutical formulation is for use in a method for treating a disease associated with abnormal protein aggregation and / or inclusion bodies formation in myocytes, neurons, or extracellular compartments, or for alleviating at least one symptom associated therewith in a human subject in need thereof, the method comprising intravenously administering to the subject a pharmaceutical formulation comprising 0.1% (w / v) to 50% (w / v) of trehalose. An injectable aqueous pharmaceutical formulation comprising a therapeutically effective amount of trehalose is also provided.SELECTED DRAWING: None

The present application relates to the technical field of separation engineering, and provides a kind of fucosyl lactose and its purification method, the purification method includes: after removing production bacteria and macromolecular substance, decoloring, desalination treatment to fucosyl lactose fermentation broth, obtain the concentrated solution with fucosyl lactose content of 35~55%, carry out continuous chromatography separation, the filler in the chromatographic column used in the continuous chromatography separation is calcium type gel resin, the temperature of chromatographic column is 50~65 DEG C, and pure water is used as eluent.The present application can obtain high yield, high purity, low impurity, no solvent residue fucosyl lactose by removing production bacteria and macromolecular substance, decoloring, desalination treatment and continuous chromatography separation under specific conditions.

The invention belongs to the technical field of functional sugar production, and discloses a preparation method for co-production of fructo-oligosaccharide and isomaltulose, which comprises the following steps: preparing sucrose into a 700-750g / L aqueous solution, heating, adding fructosyl transferase for conversion, then carrying out enzyme deactivation, cooling, adding sucrose isomerase for conversion to obtain a mixed solution containing fructo-oligosaccharide and isomaltulose, and carrying out freeze-drying to obtain the fructo-oligosaccharide and isomaltulose co-product. The mixed solution is subjected to activated carbon decoloration, ion exchange resin desalination and separation to obtain fructo-oligosaccharide and mother liquor, and the mother liquor is subjected to evaporation concentration, cooling, crystallization, centrifugation and drying to obtain isomaltulose. According to the method, the fructo-oligosaccharide and the isomaltulose can be simultaneously produced by using the cane sugar, the production process is simple, the cane sugar conversion rate is high, the product purity is high, and the production cost is low.

The application discloses a preparation method of codlane oligosaccharide, and comprises the following steps: mixing codlane polysaccharide with L molecular sieve, ball-milling and grinding, adding a solvent to dissolve, separating the L molecular sieve from the solid-liquid, and drying to obtain the codlane oligosaccharide; the L molecular sieve has a chemical composition of 1-5K2O*1.0Al2O3*5-10SiO2*1-5H2O and has straight pores; the L molecular sieve with the straight pores can selectively cut the glycosidic bond of the codlane to prepare the codlane oligosaccharide; thus, the codlane oligosaccharide can be efficiently prepared, and the L molecular sieve can be repeatedly used; the method does not need a large amount of water or other solvents to participate in the reaction, has the advantages of environmental friendliness and low cost, and has obvious advantages compared with the prior art, and is more beneficial to industrialization.

The application discloses a preparation method of xylose, which comprises the following steps: S1, adding xylanase into a xylose mother liquor for enzyme hydrolysis and viscosity reduction; S2, adding calcium hydroxide into the enzyme hydrolysis liquor and passing in CO2 to generate nanometer calcium carbonate floccus to adsorb impurities; S3, performing cross-flow filtration on the ceramic-polyether sulfone composite membrane to improve the purity of the xylose; S4, performing multistage countercurrent chromatography separation on the calcium type strong acid cation resin, and dynamically adjusting an elution gradient through online HPLC feedback; and S5, performing three-effect evaporation concentration, then stage cooling crystallization, adding alpha type xylose crystal seeds to control the crystal form; the application utilizes enzyme hydrolysis-flocculation to cooperatively reduce the viscosity of the mother liquor to greater than or equal to 45%, and the nanometer floccus has an impurity adsorption efficiency greater than or equal to 85%; a dynamic chromatography separation factor is improved to 1.8, and the xylose recovery rate is greater than or equal to 96.2%; alpha type crystal purity greater than or equal to 99.5% is obtained through gradient crystallization, and the comprehensive energy consumption is reduced; and the enzyme hydrolysis conversion of the raffinate generates xylooligosaccharide and high fructose syrup.

This invention relates to the use of a nutritional composition comprising sialylated oligosaccharides for enhancing cognitive development and learning skills in mammals. The nutritional compositioncomprises 3'-Siallylactose (3'-SL) and 6'- Siallylactose (6'-SL) in a weight ratio between 10:1 and 1:10 and is specifically for use in enhancing learning skills and / or enhancing memory function in an individual by increasing the sialic acid (Neu5Ac) concentration in the brain of said individual.

The invention discloses a marine polysaccharide degradation method and marine oligosaccharide, and relates to the technical field of marine polysaccharide degradation. The method comprises the following steps: S1, adding H2O2 into a 0.5%-2.5% (w / v) marine polysaccharide solution until the concentration of H2O2 in the solution reaches 1%-5% (w / v), and adjusting the pH value of the solution to 3-7 to obtain a first mixed solution; s2, performing microwave-ultrasonic treatment on the first mixed solution, and terminating the reaction after the microwave-ultrasonic treatment is completed to obtain a second mixed solution; s3, separating and purifying the second mixed solution, and freeze-drying to obtain the marine oligosaccharide. The method is easy and convenient to operate, green and environmentally friendly, the low-molecular-weight marine oligosaccharide can be efficiently prepared, and the prepared marine oligosaccharide is small in molecular weight and low in apparent viscosity.

This invention discloses a method for preparing a mixture of mussel oligosaccharides and the application of the product in inflammatory bowel disease. This invention uses mussel polysaccharide MP-1 as a raw material, processed by α-amylase or Bacillus subtilis (… Bacillus subtilis BNCC185269) obtained mussel oligosaccharide mixtures MOS-A and MOS-B composed of different oligosaccharides. Animal experiments have shown that the two oligosaccharide mixtures prepared by this invention can effectively alleviate intestinal inflammation. Therefore, the mussel oligosaccharide mixtures prepared by this invention are natural and safe drugs or foods for improving inflammatory bowel disease. They can effectively improve inflammatory bowel disease, are safe, non-toxic, and have no side effects. The raw materials are abundant, the steps are simple, the cost is low, and the effects are significant.

The application belongs to the field of biochemistry, and particularly relates to a preparation method of a sodium hyaluronate oligosaccharide composition, and further discloses the prepared composition and application. The preparation method of the sodium hyaluronate oligosaccharide comprises the following steps: taking macromolecular sodium hyaluronate as raw material, preparing an acidic aqueous solution, adding ethanol to perform a precipitation reaction, separating supernatant, removing impurities through an ultrafiltration membrane, performing a second ethanol precipitation reaction, separating precipitate, purifying the precipitate through a reverse-phase ODS column, and obtaining the sodium hyaluronate oligosaccharide with required performance. The preparation method has the advantages of simple and easy-to-operate preparation process, suitable reaction conditions, simple and smooth production process, greatly reduced production period and cost, and a product yield of more than 96%. The sodium hyaluronate oligosaccharide composition prepared by the method comprises disaccharide, tetrasaccharide, hexasaccharide, octasaccharide, decasaccharide and dodecasaccharide, has better product stability, excellent water solubility, good transdermal permeability, good moisturizing property, good oral absorption and other advantages.

The present disclosure describes compositions and methods including liposome or lipid nanoparticles (LNPs) including carbohydrate-based ligands that target the liver, lungs, and / or kidneys. More particularly, the present disclosure relates to compositions and methods including liposome or lipid nanoparticles (LNPs) including carbohydrate-based ligands, such as di-lactobionic acid (LBA) ligand, and a suitable cargo such as nucleic acids and small molecule to target the liver, lungs, and / or kidneys for treatment and / or diagnosis of diseases and conditions.

The present invention relates to a method for producing disaccharides using beta-glucosidase and its cofactors, and a composition for inducing enzyme production in a Trichoderma strain, which contains the produced disaccharides. Specifically, the method comprises inducing beta-glucosidase and a divalent metal ion, manganese ion (Mn ion), as a cofactor, on a glucose substrate. 2+ ), magnesium ion (Mg 2+ ), zinc ion (Zn 2+ ) or copper ions (Cu 2+ ), the disaccharide production rate can be increased and disaccharides can be obtained at a high concentration. In addition, since the composition with a high content of disaccharides produced by the production method of the present invention has an excellent effect of inducing enzyme production in Trichoderma strains, it is possible to improve the enzyme productivity of Trichoderma by applying a fermentation process that utilizes the produced disaccharides at a high concentration.

The application belongs to the technical field of sugar synthesis chemistry, and particularly relates to a synthesis method of eplactinose. The method of the application uses beta-octanoyl lactose as a raw material, and eplactinose is obtained by modifying and inverting 2-OH of a glucose fragment. The raw material is easy to obtain, the method is simple in process and low in cost, and is suitable for large-scale production.

This invention discloses a method for the combined extraction of cyperine and trehalose from Selaginella tamariscina. Using Selaginella tamariscina as raw material, this method combines the extraction of cyperine and high-purity trehalose, improving the purity of the final cyperine product. The trehalose yield is between 0.2% and 2.0%. Through solvent extraction, water washing purification, and crystallization, a pure trehalose product with a purity greater than 95.0% can be prepared. The process is environmentally friendly, generates no solid waste, and utilizes the widely available resources of Selaginella tamariscina, providing a cheap and readily available source for the preparation of trehalose-related drugs. This method is of significant importance for the comprehensive utilization and in-depth development of Selaginella tamariscina.