63results about "Disaccharides" patented technology

The invention provides a continuous crystallization method of block-shaped trehalose crystals, which comprises the following steps: stimulating trehalose syrup to crystallize to form seed syrup, growing the crystal at 60-65 DEG C for 1-2 hours, and then cooling and crystallizing to obtain the block-shaped trehalose crystals, the cooling crystallization is three-stage cooling crystallization and comprises the following steps: cooling to 50-55 DEG C at a cooling rate of 0.5-0.8 DEG C / h, then cooling to 40-43 DEG C at a cooling rate of 1-1.5 DEG C / h, and then cooling to 25-30 DEG C at a cooling rate of 2-2.5 DEG C / h. Compared with a commercially available trehalose product, the purity of the trehalose product is greater than 99.8%, the length-diameter ratio is less than 2: 1, the grain size of 97wt% or more seed crystals is greater than 60 meshes, the filtering time is shortened by 62.2%, the bulk density of the product is improved by 49.3%, the particles are uniform, and when the trehalose product is compounded with other products, the proportioning amount can be accurately mastered, and the quality of the compounded product is improved.

This invention discloses an acid-catalyzed controlled degradation and recycling method for waste polyester and polyester-cotton blended fabrics. Using waste polyester or waste polyester-cotton blended fabrics as raw materials, under the action of an acidic catalyst, in a mixed solvent composed of a polar aprotic solvent and water, the method involves heating and degradation, causing the polyester in the waste polyester or polyester-cotton blended fabrics to depolymerize or co-depolymerize, generating organic raw materials. This invention achieves complete depolymerization and recycling of polyester with a relatively low acid dosage, effectively avoiding the corrosion problems of equipment and excessive degradation of cotton fibers caused by existing technologies. It is also more conducive to the generation and recovery of glucose and 5-hydroxymethylfurfural. Furthermore, the separation steps are simple, greatly reducing separation costs, and high-yield recovery of terephthalic acid, glucose, and 5-hydroxymethylfurfural is achieved, realizing high-value recycling and utilization of various components in textiles, reducing waste, and improving resource utilization.

The invention belongs to the technical field of biomass conversion and functional food ingredients, and discloses a method for preparing galactooligosaccharide prebiotics from cellulose. The method comprises the following steps: mixing cellulose with a galactose aqueous solution, and drying to obtain galactose pre-impregnated cellulose; impregnating the cellulose pre-impregnated with galactose with an acid catalyst solution, and drying to obtain cellulose impregnated with galactose and an acid catalyst; carrying out solvent-free ball milling treatment on the cellulose impregnated with the galactose and the acid catalyst, so that the cellulose is synchronously subjected to depolymerization and grafting reaction with the galactose to obtain a crude product; and carrying out purification treatment on the crude product, and removing the residual acid catalyst to obtain the galactooligosaccharide prebiotics. According to the galactooligosaccharide prepared by the preparation method disclosed by the invention, the water solubility of the oligosaccharide is greatly improved due to the introduction of a galactose branched chain, the yield of a final product is high, and the prebiotic activity of the final product is equivalent to that of a high-cost enzymic method commercial product and even is better for a specific strain.

This invention relates to a catalyst for degrading polysaccharides, its preparation method, and its application, belonging to the field of polysaccharide degradation technology. The invention provides a method for preparing a catalyst for degrading polysaccharides, comprising: weighing fumaric acid and hydrogen peroxide, mixing them, and preparing the catalyst. The molar ratio of fumaric acid to hydrogen peroxide is 0.5–2:0.5–2. The invention also provides a catalyst for degrading polysaccharides, obtained by the above preparation method. The catalyst prepared by this invention using fumaric acid and hydrogen peroxide for the degradation of galactomannan can improve the polysaccharide degradation efficiency, resulting in high yields of low molecular weight galactomannan and galactomannan oligosaccharides, thus increasing productivity.

There is provided a resident-skin-flora-improving agent that is useful in keeping the skin in excellent condition. Specifically, there is provided a resident-skin-flora-improving agent in which sophorose is used. Sophorose is provided with functionality by which it is possible to promote proliferation of helpful bacteria that beneficially affect skin while suppressing growth of bacteria that could adversely affect the skin. The resident-skin-flora-improving agent can suitably be used for the object of maintaining balance of the resident skin flora and keeping the skin in excellent condition.

The invention discloses a preparation technology of astragalus polysaccharide camouflage monosaccharide, and relates to the technical field of biological product production, the preparation technology comprises the following steps: S1, extraction, S2, purification: carrying out purification treatment on astragalus polysaccharide extracted in the step 1, S3, degradation camouflage: dissolving the astragalus polysaccharide purified in the step 2 in a solvent, carrying out degradation treatment on the astragalus polysaccharide by adopting ultrasonic waves, and carrying out purification treatment on the astragalus polysaccharide to obtain the astragalus polysaccharide camouflage monosaccharide. A specific glucan structural unit in a polysaccharide chain is degraded and disguised into a monosaccharide or oligosaccharide structure; and S4, separating to obtain a product. Astragalus polysaccharide is directionally camouflaged as a monosaccharide / oligosaccharide structure, the recognition and uptake efficiency of astragalus polysaccharide by cancer cells is remarkably enhanced, so that the targeting of inducing apoptosis of cancer cells is improved, the structure can effectively activate and train immune cells such as dendritic cells and strengthen anti-tumor immune response, and meanwhile, the product can interfere with a metabolic pathway of cancer cells and reverse the drug resistance of cancer cells, so that the anti-tumor effect is improved. Nutrition supply is blocked to inhibit tumor growth, the production process is green and controllable, and good clinical transformation and industrialization prospects are achieved.

The invention discloses a high-efficiency separation method of breast milk oligosaccharide and application of the breast milk oligosaccharide in specific recognition of influenza A virus hemagglutinin protein.The method comprises the steps that a DE-52 weak anion exchange column, a graphite carbon column and Bio-GelP10 column chromatography are combined for use, breast milk oligosaccharide components with low abundance and high molecular weight are efficiently enriched, and the specific recognition of influenza A virus hemagglutinin protein is achieved; after derivatization labeling of a bifunctional reagent 2-amino-N-(2-aminoethyl) benzamide (AEAB), 56 breast milk oligosaccharide monomers with the purity higher than 90% are separated from the isomer level through one-dimensional amide normal phase chromatography and two-dimensional C18 reverse phase circulation chromatography, and a monomer substance library is constructed. AEAB is used as a connecting arm to construct a breast milk oligosaccharide chip, an active monomer DSLNT II which is specifically combined with four subtype influenza A virus hemagglutinin proteins is screened out in a high-throughput manner, and fine structure analysis is completed in combination with electrospray tandem mass spectrometry. According to the method, the large-scale preparation of the low-abundance breast milk oligosaccharide monomer is realized, and a key material basis and theoretical guidance are provided for researching an antiviral mechanism and developing related functional foods and medicines.

This invention relates to the use of a nutritional composition comprising sialylated oligosaccharides for enhancing cognitive development and learning skills in mammals. The nutritional compositioncomprises 3'-Siallylactose (3'-SL) and 6'- Siallylactose (6'-SL) in a weight ratio between 10:1 and 1:10 and is specifically for use in enhancing learning skills and / or enhancing memory function in an individual by increasing the sialic acid (Neu5Ac) concentration in the brain of said individual.

The invention discloses a marine polysaccharide degradation method and marine oligosaccharide, and relates to the technical field of marine polysaccharide degradation. The method comprises the following steps: S1, adding H2O2 into a 0.5%-2.5% (w / v) marine polysaccharide solution until the concentration of H2O2 in the solution reaches 1%-5% (w / v), and adjusting the pH value of the solution to 3-7 to obtain a first mixed solution; s2, performing microwave-ultrasonic treatment on the first mixed solution, and terminating the reaction after the microwave-ultrasonic treatment is completed to obtain a second mixed solution; s3, separating and purifying the second mixed solution, and freeze-drying to obtain the marine oligosaccharide. The method is easy and convenient to operate, green and environmentally friendly, the low-molecular-weight marine oligosaccharide can be efficiently prepared, and the prepared marine oligosaccharide is small in molecular weight and low in apparent viscosity.

This invention discloses a method for preparing a mixture of mussel oligosaccharides and the application of the product in inflammatory bowel disease. This invention uses mussel polysaccharide MP-1 as a raw material, processed by α-amylase or Bacillus subtilis (… Bacillus subtilis BNCC185269) obtained mussel oligosaccharide mixtures MOS-A and MOS-B composed of different oligosaccharides. Animal experiments have shown that the two oligosaccharide mixtures prepared by this invention can effectively alleviate intestinal inflammation. Therefore, the mussel oligosaccharide mixtures prepared by this invention are natural and safe drugs or foods for improving inflammatory bowel disease. They can effectively improve inflammatory bowel disease, are safe, non-toxic, and have no side effects. The raw materials are abundant, the steps are simple, the cost is low, and the effects are significant.

The application belongs to the field of biochemistry, and particularly relates to a preparation method of a sodium hyaluronate oligosaccharide composition, and further discloses the prepared composition and application. The preparation method of the sodium hyaluronate oligosaccharide comprises the following steps: taking macromolecular sodium hyaluronate as raw material, preparing an acidic aqueous solution, adding ethanol to perform a precipitation reaction, separating supernatant, removing impurities through an ultrafiltration membrane, performing a second ethanol precipitation reaction, separating precipitate, purifying the precipitate through a reverse-phase ODS column, and obtaining the sodium hyaluronate oligosaccharide with required performance. The preparation method has the advantages of simple and easy-to-operate preparation process, suitable reaction conditions, simple and smooth production process, greatly reduced production period and cost, and a product yield of more than 96%. The sodium hyaluronate oligosaccharide composition prepared by the method comprises disaccharide, tetrasaccharide, hexasaccharide, octasaccharide, decasaccharide and dodecasaccharide, has better product stability, excellent water solubility, good transdermal permeability, good moisturizing property, good oral absorption and other advantages.

The present disclosure describes compositions and methods including liposome or lipid nanoparticles (LNPs) including carbohydrate-based ligands that target the liver, lungs, and / or kidneys. More particularly, the present disclosure relates to compositions and methods including liposome or lipid nanoparticles (LNPs) including carbohydrate-based ligands, such as di-lactobionic acid (LBA) ligand, and a suitable cargo such as nucleic acids and small molecule to target the liver, lungs, and / or kidneys for treatment and / or diagnosis of diseases and conditions.

The present invention relates to a method for producing disaccharides using beta-glucosidase and its cofactors, and a composition for inducing enzyme production in a Trichoderma strain, which contains the produced disaccharides. Specifically, the method comprises inducing beta-glucosidase and a divalent metal ion, manganese ion (Mn ion), as a cofactor, on a glucose substrate. 2+ ), magnesium ion (Mg 2+ ), zinc ion (Zn 2+ ) or copper ions (Cu 2+ ), the disaccharide production rate can be increased and disaccharides can be obtained at a high concentration. In addition, since the composition with a high content of disaccharides produced by the production method of the present invention has an excellent effect of inducing enzyme production in Trichoderma strains, it is possible to improve the enzyme productivity of Trichoderma by applying a fermentation process that utilizes the produced disaccharides at a high concentration.

The application belongs to the technical field of sugar synthesis chemistry, and particularly relates to a synthesis method of eplactinose. The method of the application uses beta-octanoyl lactose as a raw material, and eplactinose is obtained by modifying and inverting 2-OH of a glucose fragment. The raw material is easy to obtain, the method is simple in process and low in cost, and is suitable for large-scale production.

Provided herein is a peptide composition that is highly stable and can be stored long term, said composition containing one or more types of peptides having an amino acid sequence represented by any of SEQ ID Nos. 1-5, or a peptide in which one or two of the amino acids of the abovementioned peptides are independent and may be substituted, deleted or added, and saccharides and an inorganic salt.

The invention provides a method for separating lactulose and lactose by using a sequential simulated moving bed chromatography system, and relates to the technical field of saccharide separation and purification. According to the invention, the sequential simulated moving bed chromatography system is used for separating and purifying lactulose, and the analysis area, the refining area, the adsorption area and the isolation area can be operated at the same time under the set temperature condition, so that the separation and purification efficiency of lactulose is remarkably improved, continuous large-scale separation and purification of lactulose can be realized, the separation and purification effect is good, the yield is high, and the product quality is good. According to the method, pure water is used as an eluent, so that analyzed and purified compounds are free of damage and toxicity, rich in source and low in cost.

To provide a polyaldehyde compound-containing composition that is less prone to heat-induced coloration, and a method for producing the same.SOLUTION: A polyaldehyde compound-containing composition contains a polyaldehyde compound blended with a predetermined amount of a low-molecular-weight aldehyde compound. Consequently, the polyaldehyde compound-containing composition is less prone to heat-induced coloration in its solid state.SELECTED DRAWING: None

The present invention relates to a method of removing calcium citrate from a liquid dairy stream. In particular, the present invention relates to a method of removing calcium citrate from a liquid dairy stream wherein the liquid dairy stream has a pH below 6.2 or is optionally adjusted to be below 6.2, and wherein said liquid dairy stream having a pH below 6.2 is subjected to a step of precipitation of calcium citrate. Subsequently, the precipitated calcium citrate can be separated.

A composition includes a sugar source and inositol. The sugar or a sugar source is one more sugars select from the group consisting of glucose, sucrose, sucrolose, tagatose, galactose, high fructose com syrup, fructose, isoglucose, and rhamnose. The composition of sugar and inositol has an unique properties that prevents or limits the signaling of TNF-a and associated pro-inflammatory cytokines when metabolized by an individual consuming the composition. Accordingly, the composition can be advantageously used to control blood glucose levels, treat diabetes and related conditions as well as treat diseases based on an inflammatory response.

Provided are a usage method for a topical agent composition and a topical agent composition that make it possible for an oil-soluble component of the topical agent composition to achieve effective penetration. According to the present invention, a usage method for a topical agent composition involves applying a topical agent composition that includes water and solubilized micelles that encapsulate an oil-soluble component in a surfactant to a body surface, applying energy to the body surface to which the topical agent composition has been applied from above the topical agent composition using a beauty apparatus, and allowing the solubilized micelles to penetrate in from the body surface.

A citrate composition is provided for use in 3D printing of end products, e.g., scaffolds. The disclosed composition incorporates water soluble salts and / or sugars into a pre-polymer to improve the viscosity for 3D printing, increase the porosity of the resulting scaffold, and improve the handling of the citrate based bioceramic compositions.

The invention belongs to the technical field of food, and particularly relates to a preparation method of crystalline lactulose. The preparation method of the crystalline lactulose comprises the following steps: (1) weighing lactulose powder, dissolving in water, heating and stirring to obtain a lactulose solution; (2) adding a composite regulating agent into the lactulose solution obtained in the step (1), and monitoring the viscosity to obtain a mixed solution; (3) adding lactulose crystal seeds into the mixed solution obtained in the step (2) for induced crystallization; then gradient cooling crystallization is adopted; (4) after crystallization is completed, carrying out heat preservation, separation, washing and drying to obtain a lactulose crystal product, and collecting mother liquor for cyclic utilization; according to the preparation method of the crystalline lactulose, the viscosity of the mother liquor can be reduced, the product purity is high, and the yield is high.

The invention belongs to the technical field of chromatographic separation, and particularly relates to a chromatographic column combined lactulose separation method which comprises the following steps: A1, catalytically converting lactose into lactulose to obtain a lactulose conversion solution; a2, adjusting the pH value of the lactulose conversion liquid, performing nanofiltration to obtain a clear liquid and wastewater, performing ion exchange on the clear liquid, and concentrating to obtain a feed liquid; a3, conveying the feed liquid into a first-stage chromatographic separation system, and carrying out first-stage washing separation to obtain a first-stage product containing more than 94 wt% of lactulose and a recovery liquid; and A4, carrying out decoloration, ion exchange and evaporation concentration on the primary product, conveying the primary product into a secondary chromatographic separation system, carrying out secondary washing separation to obtain a secondary product containing more than 99 wt% of lactulose and a waste liquid, and carrying out fixed bed ion exchange, decoloration and evaporation concentration on the secondary product to obtain a lactulose product. According to the method, 99 wt.% of lactulose products are obtained, and a green and environment-friendly process route is opened up for industrial preparation of high-purity lactulose.

The invention relates to the technical field of food additive production, and discloses a lactitol preparation method, which comprises: sequentially carrying out ultrafiltration, nanofiltration and resin treatment on whey to obtain a three-stage lactose liquid; carrying out catalytic hydrogenation on the third-grade lactose liquid at a low pressure of 3-4 MPa to prepare a lactitol liquid; refining the lactitol liquid through an activated carbon fiber membrane and the like; the refined lactitol liquid is sequentially subjected to three-step continuous crystallization: firstly, carrying out evaporative crystallization separation to obtain anhydrous lactitol and an anhydrous mother solution; carrying out evaporative crystallization and cooling crystallization on the anhydrous mother solution, and separating to obtain lactitol monohydrate and a monojellyfish solution; and carrying out cooling crystallization on the monojellyfish liquid, and separating to obtain the lactitol dihydrate. According to the method, the content of lactose protein is reduced to a lower level through multi-step purification, and in addition, the hydrogenation process is carried out when the content of solids is low, so that the hydrogenation pressure can be reduced, and the production safety can be improved; and the mother liquor is subjected to continuous gradient crystallization, so that gradient utilization of heat energy and efficient recovery of products are realized.

The invention relates to the technical field of food engineering and chemical separation, and discloses a lactose solution decoloring method based on strongly acidic macroporous adsorption resin, which comprises the following steps: pretreating strongly acidic macroporous adsorption resin with sulfonic acid groups; enabling a lactose solution to be decolored with the mass concentration of 30-50% to flow through the pretreated resin at the volume flow rate of 1.0-2.0 BV / h under the conditions that the pH value is 3.0-6.0 and the temperature is 70-90 DEG C, and carrying out adsorption decoloring; regeneration treatment is carried out on the resin saturated through adsorption, and the regeneration treatment sequentially comprises hot water flushing, alkaline solution elution and acid solution treatment. The decolorization process can be performed at high temperature, so that the problem of blockage of a pipeline or a column bed caused by easy crystallization of the high-concentration lactose solution at low temperature is effectively avoided, efficient decolorization and deproteinization of the lactose solution are realized, and meanwhile, the method has the technical effects that the lactose loss rate is low, and the resin can be recycled.

This invention provides a heterogeneous unsupported tin-based catalyst, its preparation method, and its application, belonging to the field of tin-based catalyst materials. The preparation method of the heterogeneous unsupported tin-based catalyst provided by this invention includes the following steps: first, tin tetrachloride and an aqueous solution of an organic alcohol are mixed, followed by the addition of a soluble aluminum salt, and then the mixture is allowed to stand for a first time to obtain a mixed solution; second, the mixed solution is mixed with ammonia water, and the mixture is allowed to stand for a second time, followed by high-temperature hydrolysis and drying to obtain the heterogeneous unsupported tin-based catalyst; the temperatures of the first and second standing periods are both -12 to -20°C. The heterogeneous unsupported tin-based catalyst prepared by the method provided by this invention is stable (it will not transform into tin dioxide), and can convert glucose to lactic acid with a yield of over 90%. Furthermore, the catalyst can achieve 93% of its initial efficiency even after 10 repetitions, exhibiting good stability and catalytic effect.

The invention discloses an extraction method and application of alginate oligosaccharide with high alginic acid content and specific polymerization degree distribution, and relates to the technical field of high-value utilization of marine biological resources. The alginate oligosaccharide with alginic acid content of more than 87%, polymerization degree of 3-7 and terminal guluronic acid unit proportion of more than or equal to 60% is prepared through fractional precipitation of raw materials, enzyme-membrane coupling and dynamic membrane separation and purification. The alginate oligosaccharide extracted by the extraction method disclosed by the invention can be independently used as a biostimulant, can also be compounded with other substances, can effectively promote root growth, resist stress and increase yield in agriculture, and is high in activity and stable in effect.

Provided is a method for preparing lactulose using immobilized β-galactosidase. The method comprises the following steps: pretreating a macroporous weakly basic styrene-based anion exchange resin; mixing the pretreated resin with a β-galactosidase solution and adding same to a buffer, adsorbing same using a shaker, and leaving same to stand after adsorption; then adding a glutaraldehyde solution for cross-linking, and discarding the supernatant to obtain an immobilized enzyme column; adding a magnesium ion solution to a mixed solution of lactose and fructose, and then converting same by means of the immobilized enzyme column to obtain a lactulose conversion solution; and sequentially performing decolorization, ion exchange, vacuum concentration, and sequential simulated moving bed chromatography separation on the lactulose conversion solution to obtain a lactulose solution.

The utility model provides a crystallized trehalose recrystallization device which comprises a rack and a crystallization kettle, the crystallization kettle is horizontally placed, stirring blades are arranged in the crystallization kettle, and materials are stirred by the stirring blades in the recrystallization process, so that the materials can be fully stirred, and the problem that the crystallization quality and efficiency are ordinary due to non-uniform stirring is solved. Flexible scrapers are arranged on the outer sides of the stirring blades, so that materials attached to the inner wall of the crystallization kettle can be effectively cleaned, crystallization materials are prevented from being accumulated on the inner wall of the crystallization kettle, efficient operation of equipment is further maintained, and the crystallization efficiency and the product quality are further improved. In addition, the first cooling pipe and the second cooling pipe which are spirally arranged on the outer side of the crystallization kettle realize efficient convection cooling through the water inlet and the water outlet in opposite directions, and uniform temperature distribution in the kettle is ensured, so that the crystallization effect is optimized, and the product purity is improved. And after the material crystallization is completed, the telescopic assembly drives the crystallization kettle to lift, so that the discharge port is positioned at a lower position, and the material can be discharged more easily.

The invention relates to the technical field of edible mushroom polysaccharide extraction, in particular to agrocybe cylindracea beta-1, 3-glucan as well as a preparation method and application thereof. The preparation method comprises the following steps: adding agrocybe cylindracea sporocarp and water, extracting at 80-90 DEG C for 2-3 hours, and extracting for 2-3 times; after extraction, combining the two filtrates to obtain an extracting solution I; drying the residues, adding the dried residues into an alkaline solution, extracting for 2-3 times at 70-90 DEG C, combining the two extracting solutions, and adjusting the pH value to be neutral to obtain an extracting solution II; combining the extracting solution I and the extracting solution II to obtain a total extracting solution; carrying out micro-filtration membrane treatment on the total extracting solution, collecting a non-permeated solution, freeze-drying, re-dissolving in water, adding an ethanol solution with the concentration of 90-95%, and carrying out alcohol precipitation treatment; and collecting the precipitate, and freeze-drying to obtain the agrocybe cylindracea beta-1, 3-glucan. The agrocybe cylindracea beta-1, 3-glucan has the advantages that the agrocybe cylindracea beta-1, 3-glucan can be used for improving the innate immune cell function by training immunity and enhancing the body immunity.