1 results about "Isoamylase" patented technology

This invention discloses a multi-step sequential enzymatic hydrolysis combined with multi-stage membrane purification method for preparing kudzu oligosaccharides, relating to the field of functional food processing technology. The preparation method includes the following steps: adding kudzu powder to water to prepare a starch slurry, then liquefying it using α-amylase, followed by enzymatic hydrolysis using debranching enzymes and glycoside hydrolases to obtain an enzymatic hydrolysate; performing membrane separation on the enzymatic hydrolysate to obtain a fraction with a molecular weight higher than 1 kDa and lower than 5 kDa, followed by drying to obtain the kudzu oligosaccharides; the debranching enzymes are pullulanase and isoamylase; the glycoside hydrolases are β-glucosidase or α-transglucosidase. This preparation method can effectively increase the proportion of the DP3-15 fraction of kudzu oligosaccharides, achieving a product purity of over 91% and a total yield exceeding 22%.