Seed-crystal-induction-and-dual-enzyme composite preparation method for RS3 type long-shaped-rice-based resistant starch

A technology for resistant starch and indica rice, applied in the direction of fermentation, etc., can solve the problems of influence, amylose loss, unable to obtain crystal particle products with particle size distribution, etc., and achieve the effect of improving the application field, improving the yield and reasonable processing cost.

Inactive Publication Date: 2017-03-08
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In recent years, RS 3 The preparation methods mainly include moist heat method, enzymatic method, microwave method, ultrasonic-assisted method, etc., but neither a single preparation method nor a combination of several methods can obtain crystal particle products with concentrated particle size distribution, and the currently used enzymatic method Prepare RS 3 Both use liquefaction enzymes combined with debranching enzymes. The liquefaction enzymes not only cause the loss of amylose in the raw materials, but also a large amount of dextrin produced in the reaction process also affects the RS 3 generate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] RS 3Preparation of wet seed crystals of potato resistant starch: take potato starch as raw material, add distilled water to prepare potato starch milk with a starch mass fraction of 10%, pregelatinize in an 80% constant temperature water bath, until the potato starch milk becomes a transparent gel Then use a high-pressure steam sterilizer to treat at 110°C and 0.05MPa for 30 minutes, cool to room temperature, and refrigerate at 4°C for 24 hours, and adjust the pH value to 1.5; Add pepsin at the ratio of 12U pepsin, and treat it in a constant temperature water bath at 38°C for 2 hours, and adjust the pH value to 6.0; ℃ constant temperature water bath for 30 minutes, adjust the pH value to 4.0; then, add glucoamylase according to the ratio of 1g raw potato starch plus 150U glucoamylase, treat in 58 ℃ constant temperature water bath for 2 hours, cool to room temperature; finally add 95% mass concentration of Ethanol was centrifuged at 4000r / min for 35min, the supernatant ...

Embodiment 2

[0029] RS 3 Preparation of wet seed crystals of potato resistant starch: take potato starch as raw material, add distilled water to prepare potato starch milk with a starch mass fraction of 10%, pregelatinize in an 85% constant temperature water bath, until the potato starch milk becomes a transparent gel Then use a high-pressure steam sterilizer to treat at 121°C and 0.15MPa for 20min, cool to room temperature, and refrigerate at 4°C for 48h, adjust the pH value to 1.5; add 1g of raw potato starch to potato starch milk Add pepsin in the proportion of 20U pepsin, treat it in a constant temperature water bath at 40°C for 1 hour, and adjust the pH value to 6.5; Treat in a constant temperature water bath for 20 minutes, adjust the pH value to 4.5; then, add glucoamylase at the ratio of 1g of raw potato starch plus 150U glucoamylase, treat in a constant temperature water bath at 60°C for 1 hour, and cool to room temperature; finally add 95% ethanol , centrifuged at 4500r / min for ...

Embodiment 3

[0032] RS 3 Preparation of wet seed crystals of potato resistant starch: take potato starch as raw material, add distilled water to prepare potato starch milk with a starch mass fraction of 10%, and pregelatinize it in an 82% constant temperature water bath until the potato starch milk becomes a transparent gel Treated in a high-pressure steam sterilizer at 115°C and 0.10MPa for 25 minutes, cooled to room temperature, refrigerated at 4°C for 36 hours, and adjusted to pH 1.5; in potato starch milk, add 1g of raw potato starch Add pepsin at the ratio of 16U pepsin, treat in a constant temperature water bath at 39°C for 1.5h, and adjust the pH value to 6.2; ℃ constant temperature water bath for 25 minutes, adjust the pH value to 4.2; then, add glucoamylase according to the ratio of 1g raw potato starch plus 180U glucoamylase, treat in 59 ℃ constant temperature water bath for 1.5h, cool to room temperature; finally add 95% mass concentration The ethanol was centrifuged at 4200r / m...

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Abstract

The invention relates to a seed-crystal-induction-and-dual-enzyme composite preparation method for RS3 type long-shaped-rice-based resistant starch. Pregelatinization processing is carried out on long-shaped-rice-based starch by using a microwave technology and stepwise debranching enzymolysis is carried out on the starch after microwave processing by using isoamylase and pullulanase; after increasing of the content of amylose in a raw material system, an RS3 type potato resistant starch seed crystal is added, so that aggregation and recrystallization of the long-shaped-rice amylose are carried out rapidly on a low-supersaturation-degree condition to generate RS3 type long-shaped-rice-based resistant starch. The prepared resistant starch has characteristics of short devitrification time, high yield, and centralized crystal grain size distribution; and thus the long-shaped rice additional value is improved and the application field is expanded. Moreover, the starch material employed in the method is available; production can be carried out throughout the year without being limited by seasons; the processing cost is reasonable; and the prepared product has the broad market prospects.

Description

technical field [0001] The present invention relates to RS 3 The preparation of type resistant starch, specifically relates to a RS 3 Preparation of RS by the step-by-step debranching enzymatic hydrolysis process of type potato resistant starch as seed crystal combined with isoamylase and pullulanase 3 Method for Resistant Starch in Indica Rice. Background technique [0002] Resistant starch refers to the general term for starch and its degradation products that are not digested by the small intestine of healthy humans but can be digested and absorbed in the large intestine. RS 3 Type resistant starch is also known as retrogradation or recrystallization starch. The main principle of preparation is to gelatinize the starch and then quickly refrigerate and age it back to life. Under the treatment conditions of the external heat source, the molecular chain absorbs water and gelatinizes, and the crystalline high-polymer state collapses. High-energy disordered state; starch m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/16C12P19/04C08B30/12
CPCC08B30/12C12P19/04C12P19/16
Inventor 王燕欧阳梦云
Owner HUNAN AGRICULTURAL UNIV
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