33 results about "Starch synthesis" patented technology

The invention discloses a grain specific promoter in the early stage of rice filling and application thereof, the promoter is named as proASP1, and the nucleotide sequence of the promoter is shown as SEQ ID NO: 1. The promoter has highly strict spatio-temporal expression specificity, the activation expression of the promoter is only limited in grains in the early stage of rice filling (4-12 days after flowering), especially the expression of genes is efficiently driven in endosperm and aleurone layers, and the expression is not leaked in other tissues such as roots, stems, leaves, leaf sheaths and glumes and grains in the middle and later stages of filling. The invention provides a basic and precious molecular tool for gene function research, quality improvement (such as protein and starch synthesis regulation) and bioreactor development in a key window period of rice grain development, and has important scientific research and application values.

The invention belongs to the technical field of genetic engineering, and discloses an IbGSTL2 protein and application thereof in regulation and control of starch synthesis. The technical problem to be solved by the invention is how to regulate and control the starch content of plants. The IbGSTL2 protein disclosed by the invention is a protein of which the amino acid sequence is SEQ ID No. 1. Experiments prove that a plant over-expressed with the IbGSTL2 gene has higher starch content and amylopectin proportion, and the activity of a key enzyme synthesized with amylopectin is improved; the plant with interference expression of the IbGSTL2 gene has lower starch content and amylopectin proportion, and the activity of a key enzyme synthesized with amylopectin is reduced. The result shows that the IbGSTL2 protein and the coding gene not only can regulate and control the starch content, but also can regulate and control the ratio of amylopectin to amylose.

The invention discloses an application of a TaAP2-4 gene in regulating and controlling starch content of wheat grains, which is used for enhancing the expression quantity of the TaAP2-4 gene to improve the starch content of the wheat grains and weakening the expression quantity of the TaAP2-4 gene to reduce the starch content of the wheat grains. The TaAP2-4 gene is located on a chromosome 4 of a wheat genome, and three copies, namely TaAP2-4-A, TaAP2-4-B and TaAP2-4-D, are formed on the chromosome 4. According to the invention, a Crispr / Cas9 gene editing vector for knocking out the TaAP2-4 gene is constructed by adopting a reverse genetics method, the TaAP2-4 gene is successfully knocked out in a wheat material by adopting an agrobacterium-mediated method, and the starch content of a knocked-out mutant is reduced, so that the condition that the TaAP2-4 gene plays a positive regulation role in a wheat grain starch synthesis process is known. In practical application, directional knockout mutation of the genome level TaAP2-4 gene can be achieved through a gene transformation process, the gene knockout mutation method can be used for verifying functions of the TaAP2-4 gene, and materials are provided for mining other starch synthesis related genes.

This invention discloses a rice starch synthesis-related gene, OsFLO17, its encoded protein, and its applications. Through phenotypic analysis of the rice endosperm mealy mutant flo17 and preliminary localization of the target gene, the starch synthesis-related protein OsFLO17 was ultimately cloned. This protein consists of the amino acid sequence shown in SEQ ID NO.3. The starch granule development-related protein of this invention affects the rice endosperm starch synthesis process. Introducing the encoding gene of this protein into plants with abnormal starch granules can yield transgenic plants with normal starch filling. Therefore, the protein and its encoded gene of this invention can be applied to plant genetic improvement.

The invention relates to the technical field of gene engineering and molecular breeding, in particular to a DcaWRKY2 gene for regulating and controlling anthocyanin synthesis and starch accumulation of dendrobium officinale under microgravity and gravity conditions and application of the DcaWRKY2 gene. The DcaWRKY2 gene provided by the invention is a WRKY gene capable of inhibiting anthocyanin synthesis and starch accumulation in dendrobium officinale under the conditions of microgravity and gravity. In the specific embodiment of the invention, the function of the DcaWRKY2 gene is verified through a virus-induced gene silencing (VIGS) technology, and the result shows that the existence of the gene can inhibit starch synthesis and anthocyanin accumulation of dendrobium officinale leaves; in dendrobium officinale, the content of starch and anthocyanin in dendrobium officinale leaves can be obviously increased through targeted silencing of a target gene (DcaWRKY2 gene); meanwhile, a large amount of starch and anthocyanin are accumulated in the dendrobium officinale leaves, so that the starch content of the leaves can be obviously increased, and the leaves turn red.

The invention belongs to the field of plant engineering technology and crop germplasm resource innovation, and particularly relates to a rice BEIIb gene mutant, application of the rice BEIIb gene mutant in improving rice resistant starch and an improving method. A mutant material with high resistant starch content is created by knocking out the rice BEIIb gene, and compared with a wild type, mutant seeds show an obvious chalky phenotype, composite starch particles are abnormal in a spherical or ellipsoidal shape, the amylose content is remarkably increased, the protein content is remarkably reduced, the resistant starch content is remarkably increased, and the resistant starch content is remarkably reduced. The hardness of cooked rice is remarkably increased, and the elasticity is remarkably reduced. Through transcriptomics analysis, the protein mainly participates in biological pathways such as carbohydrate metabolism, chloroplast photosynthesis, calmodulin combination and secondary metabolite synthesis, and the expression level of endosperm starch synthesis related genes is obviously changed. The method can provide theoretical basis and method reference for breeding of high-quality rice varieties with high resistant starch.

The invention belongs to the field of gene engineering, and relates to a chalkiness-related protein OsGAPDH of rice endosperm as well as a coding gene and application of the chalkiness-related protein OsGAPDH. The protein provided by the invention is a protein as shown in (a) or (b): (a) a protein consisting of an amino acid sequence as shown in SEQ ID NO: 3 in a sequence table; and (b) a protein which is obtained by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence of SEQ ID NO: 3, is related to plant starch synthesis and is derived from SEQ ID NO: 3. The protein provided by the invention affects normal development of rice endosperm. The coding gene of the protein is introduced into Ningjing No.7 wild type, so that a transgenic plant of which the chalkiness of endosperm is obviously reduced can be cultivated. The protein and the coding gene thereof can be applied to plant genetic improvement.

The invention relates to a protein SSIIIa related to the content of plant resistant starch, a coding gene and an application thereof. The coding gene of the protein can control the content of resistant starch in rice. SSIIIa The gene can be used as a molecular marker to assist breeding and use genetic engineering methods to cultivate new rice varieties with high resistant starch content. The present invention provides the amino acid sequence of a protein related to plant resistant starch synthesis and the nucleotide sequence of its encoding gene, and also provides an expression cassette containing the encoding gene, a recombinant expression vector, a recombinant bacterium, a transgenic cell line, and a primer pair for amplifying the encoding gene. It also provides the use of the encoding gene of the protein related to plant resistant starch synthesis in the preparation of transgenic plants related to resistant starch synthesis and related transgenic methods. The present invention provides a powerful means for improving the quality and health function of rice, and has important theoretical significance and huge application potential.

This invention discloses a tobacco-derived α-glucan phosphorylase, its mutants, and their applications. Addressing the problems of low catalytic efficiency, poor stability and adaptability, and difficulty in heterologous expression of key enzyme elements in cellulose-to-starch synthesis, this invention utilizes metagenomics and big data analysis of public databases, along with bioinformatics methods, to precisely identify novel key enzyme elements for cellulose degradation and starch synthesis. An α-glucan phosphorylase from tobacco was identified and found to have better enzyme activity and starch synthesis function than the potato-derived PGP. Furthermore, this invention involves a single point mutation of the Cap domain of the α-glucan phosphorylase. The results show that the single point mutant obtained by mutating the tobacco-derived α-glucan phosphorylase with the amino acid sequence shown in SEQ ID No. 1 by the S524H single point mutation has significantly higher enzyme activity and expression level than the wild-type α-glucan phosphorylase.

The invention discloses a gene OsFLO17 related to rice starch synthesis as well as an encoding protein and application of the gene OsFLO17. Through phenotypic analysis of a rice endosperm powder mutant flo17 and preliminary positioning of a target gene, the protein OsFLO17 related to starch synthesis is finally obtained through cloning, and the related protein is composed of an amino acid sequence shown in SEQ ID NO.3. The starch granule development related protein provided by the invention influences the synthesis process of rice endosperm starch, and a transgenic plant with normal starch filling can be obtained by introducing the coding gene of the protein into a plant with abnormal starch granules. Therefore, the protein and the gene coded by the protein can be applied to plant genetic improvement.

The present application relates to a kind of starch synthesis regulatory gene OsMFP1 and its encoding protein and application, belong to plant genetic engineering technical field, the gene has the nucleotide sequence as shown in SEQ ID NO.1, its encoding protein has the amino acid sequence as shown in SEQ ID NO.2, the function loss of gene OsMFP1 leads to the total starch content in rice grain, amylose content and amylopectin content reduction, and lead to the yield reduction of rice.The function of OsMFP1 gene in the process of starch synthesis and its mechanism of action are studied in the present application, and it is found that the function loss of OsMFP1 gene can lead to the decrease of total starch, amylose and amylopectin content in grain, which provides important theoretical value for the improvement of rice quality.

The invention discloses application of an IbARF1 gene in regulation and control of sweet potato starch synthesis, and belongs to the field of gene genetic engineering. Through overexpression of the IbARF1 gene in sweet potatoes, it is found that transgenic sweet potato roots with overexpression of IbARF1 have lower total starch content and amylose content, and sweet potato roots in transgenic plants with interference expression of the IbARF1 gene have higher starch content and amylose content, which indicates that the IbARF1 gene not only can regulate and control the total starch content of sweet potatoes, but also can regulate and control the amylose content of sweet potatoes. The amylose content can also be regulated and controlled. Through the gene, the total starch content, the amylose content or the ratio of amylose to amylopectin of sweet potatoes can be regulated and controlled, and a new gene and a new method are provided for cultivating new sweet potato varieties with different starch contents.

The invention discloses a method for predicting a transcription factor with a regulation effect in corn endosperm development, which comprises the following steps of: screening out an endosperm development key transcription factor by carrying out conjoint analysis of a transcriptome and chromatin open region sequencing technology on a plurality of early development stages of grains. The method provided by the invention can efficiently and accurately identify the corn endosperm development key transcription factor and a new transcription factor capable of regulating starch synthesis and Zein genes.

The present invention relates to the technical field of genetic engineering and molecular breeding, and in particular to a DcaWRKY2 gene and its application in regulating starch accumulation and changing leaf color. The present invention isolates the DcaWRKY2 gene from Dendrobium officinale, and its nucleotide sequence is shown in SEQ ID NO.1. In a specific embodiment of the present invention, the function of the gene is verified by virus-induced gene silencing technology in Dendrobium officinale leaves, and the gene can regulate starch synthesis, anthocyanin accumulation and leaf color. Therefore, the DcaWRKY2 gene provided by the present invention can enrich and improve the transgenic material library of high starch content and red leaf traits of Dendrobium officinale leaves under gravity and microgravity, and provides strong support for the molecular breeding of high-quality Dendrobium officinale and red leaf traits of Dendrobium officinale under gravity and microgravity in the future.

The invention discloses a corn ZmHY5L gene and application of the corn ZmHY5L gene in improvement of corn yield and quality, and belongs to the technical field of molecular breeding. The invention discloses the effect of the corn ZmHY5L gene in regulating and controlling the yield and the quality of corn for the first time, and a plant with the corn ZmHY5L gene mutated / knocked out has the advantages that the seed volume is increased, and the hundred-grain weight is increased; the particle combined starch synthase protein is reduced, the total starch content is not changed, the amylopectin proportion is increased, and the waxiness of the mouth feel is enhanced; a new functional gene is provided for early revealing a regulation and control network of corn yield and starch composition and genetic improvement of corn quality; technicians in the field can realize early detection of corn yield and quality by investigating the expression quantity of the ZmHY5L gene, and the method can be used for genetic engineering breeding of high-yield and high-quality corn, and has important significance for assisting molecular design breeding, accelerating the breeding process and reducing the breeding cost and workload.

The invention discloses a tagatose-6-phosphate phosphatase mutant and application of the tagatose-6-phosphate phosphatase mutant in production of D-tagatose, and belongs to the technical field of enzyme engineering. The tagatose-6-phosphate phosphatase mutant disclosed by the invention is obtained by mutating wild type tagatose-6-phosphate phosphatase of which the amino acid sequence is shown as SEQ ID NO.5, and the mutation site is selected from at least one of the 45th site, the 108th site, the 168th site and the 186th site. After the mutant is coupled with isoamylase, dextran phosphorylase, glucose phosphate mutase, glucose-6-phosphate isomerase, 4-alpha-glucosyltransferase and tagatose-6-phosphate isomerase, the yield of D-tagatose synthesized by substrate starch is increased, the accumulation amount of by-products glucose and fructose is low, and the yield of D-tagatose synthesized by substrate starch is increased. Therefore, the production efficiency is greatly improved, and the separation and purification cost is reduced. Therefore, the mutant disclosed by the invention is suitable for industrial production and has a good application prospect.

A TaIIP1 gene for regulating wheat grain starch synthesis and use thereof. The present invention belongs to the technical field of gene function regulation. Firstly, the important effect of the wheat isoamylase TaISA1 gene on the quality of grain starch synthesis is clarified. Moreover, the wheat sucrose non-fermentation-related protein kinase TaIIP1 gene, which has a positive regulatory effect on the grain starch synthesis and the phenotype of grains, is found by means of the yeast two-hybrid method and other methods, the function thereof is verified by means of overexpression, and excellent variants thereof are found by means of haplotype analysis. The present invention provides a theoretical basis and excellent gene resources for the use of the TaIIP1 gene to create or improve high-quality wheat materials.

The present invention relates to the field of genetic engineering and molecular breeding technology, and in particular to a DcaWRKY2 gene for regulating anthocyanin synthesis and starch accumulation in Dendrobium officinale under microgravity and gravity conditions and its application. The DcaWRKY2 gene provided by the present invention is a WRKY gene that inhibits anthocyanin synthesis and starch accumulation in Dendrobium officinale under microgravity and gravity conditions. In a specific embodiment of the present invention, the function of the DcaWRKY2 gene was verified by virus-induced gene silencing (VIGS) technology, and the results showed that: the presence of the gene inhibits starch synthesis and anthocyanin accumulation in Dendrobium officinale leaves; in Dendrobium officinale, by targeted silencing of the target gene (DcaWRKY2 gene), the content of starch and anthocyanin in the leaves of Dendrobium officinale can be significantly increased; at the same time, due to the large amount of accumulation of starch and anthocyanin in the leaves of Dendrobium officinale, the starch content of the leaves can be significantly increased and the leaf color becomes red.

The invention belongs to the technical field of bioengineering, and particularly relates to kluyveromyces marxianus engineering bacteria for synthesizing inositol by synchronously saccharifying and fermenting starch and application of the kluyveromyces marxianus engineering bacteria. According to the kluyveromyces marxianus engineering bacterium, a glucose-6-phosphate isomerase PGI gene, a glucose-6-phosphate dehydrogenase ZWF1 gene, an inositol oxygenase MIOX5 gene and an inositol transporter protein ITR2 gene of a kluyveromyces marxianus FIM1 delta ura3 strain are sequentially knocked out, and the kluyveromyces marxianus engineering bacterium is obtained. And transferring into a carrier pUKDN115-Ptef-TbIPS-Ttef-Pgp-EcIMP-Tgp-Pinu-alpha BadGLA-Tinu, wherein the carrier is used for simultaneously expressing a trypanosoma buchneri inositol phosphate synthase TbIPS gene, an escherichia coli inositol monophosphate enzyme EcIMP gene and a saccharifying enzyme BadGLA of adenine yeast, so as to obtain the trypanosoma buchneri inositol phosphate synthase TbIPS-Ttef-Pgp-EcIMP-Tgp-Pinu-alpha BadGLA-Tinu. The Kluyveromyces marxianus engineering strain is used as a fermentation strain, starch is used as a raw material, the inositol is produced through simultaneous saccharification and fermentation, and the yield of the inositol in supernatant can reach 45.5 g / L.

The invention discloses a high-level starch synthesis microorganism, relates to genetic modification for improving the starch synthesis capacity of the microorganism and a genetic engineering bacterium for efficiently synthesizing starch, and belongs to the technical field of biotechnology and genetic engineering. The method comprises the following steps: by taking microorganisms into which exogenous glucose-1-phosphoadenosine transferase and starch synthetase genes are introduced as initial strains, optimizing a starch synthesis route, strengthening starch synthesis precursor supply, weakening a starch degradation process, finely blending a carbon metabolic flow and transforming the form and cell size of the strains; the starch synthesis and accumulation capability of the strain is improved. The genetic modification disclosed by the invention can greatly improve the yield of starch synthesized by microbial cells, the carbon source conversion rate and the production intensity, produces the genetically engineered bacterium which is high in industrial attribute and is capable of efficiently synthesizing starch, and can be applied to industrial biological manufacturing of starch.

The invention discloses an early sowing cultivation method for improving sorghum quality and meeting the brewing requirement of Maotai-flavor liquor in the southwest region, and belongs to the technical field of crop cultivation and brewing raw material quality improvement. According to the application, the sowing time of the sorghum is 15-30 days earlier than the local conventional sowing time (March 20th-April 10th), so that the whole growth period of the sorghum is in a composite environment of lower average temperature and longer illumination, and seedling-stage cold-resistant and drought-resistant cultivation measures are implemented in a matched manner. The method is simple and easy to implement, grain quality is directionally optimized from the angle of growth environment regulation and control, sudden rise of spring temperature is avoided in the filling period, amylose synthase is inhibited, and amylopectin accumulation is promoted; starch polymerization is slowed down at low temperature, and photosynthetic product accumulation is assisted by long-time illumination, so that starch particles are loosened, gelatinization viscosity is reduced, and multiple rounds of fermentation are adapted. Meanwhile, metabolism of phenylpropane is activated through long-time illumination, synthesis of polyphenol such as tannin is promoted, the brewing quality is improved, and application prospects are achieved.

The present invention relates to the field of genetic engineering technology, in particular to a method for regulating taro starch synthesis. CeTCP14 Gene and its application. The present invention provides a gene for regulating plant starch synthesis CeTCP14 The nucleotide sequence of the gene is shown in SEQ ID NO.1. CeTCP14 Gene, which can significantly reduce the starch content in taro corms and reduce the expression of starch synthesis-related genes in taro corms; overexpression in tobacco CeTCP14 The gene can significantly increase the starch content in tobacco leaves and increase the expression of starch synthesis-related genes in tobacco leaves. CeTCP14 Genes play an important role in the positive regulation of plant starch synthesis. The present invention can not only be applied to the biosynthesis of starch in taro, but also provide technical support for high-quality and high-yield cultivation and molecular breeding of taro.

The present application belongs to the technical field of genetic breeding, and particularly relates to a method for efficiently creating new germplasm of maize with low mycotoxin and high starch content by using gene editing technology and application thereof. The method comprises using CRISPR / Cas9 multi-gene editing technology and genetic transformation method, and in the genetic transformation process, one or more of betaine and calcium chloride are contained in the infection medium. On the basis of the research on the existing starch synthesis and ear rot related functional genes, the high-throughput gene editing technology is used to efficiently and randomly aggregate a large number of excellent sites, and in the genetic transformation process, appropriate concentrations of betaine and calcium chloride are added, so that more numbers are edited in a single transgenic event, the efficiency is better, the diversity degree is effectively improved, more excellent site combination modes are generated, a large number of new germplasm with high starch content and resistance to diseases such as ear rot are efficiently and large-scale produced, and the method has good application value in gene editing breeding.

The invention provides a glucan phosphorylase mutant with improved thermal stability and application thereof, and relates to the technical field of enzyme engineering. Amino acids at the 40th site, the 271th site and the 707th site of wild-type glucan phosphorylase with an amino acid sequence shown as SEQ ID NO.1 from Solanum tuberosum are mutated to obtain the glucan phosphorylase mutant with improved thermal stability, the enzyme activity is improved by 39% at 37 DEG C, and the enzyme activity can still be kept about 60% after heat preservation is carried out for 1 hour at 60 DEG C; the amylose can be produced through conversion by taking glucose 1-phosphate as a substrate, and the conversion rate can be up to 98%. When the glucan phosphorylase mutant is applied to production of amylose, the reaction efficiency can be improved, the production period can be shortened, the production cost is further reduced, and the glucan phosphorylase mutant has a good application prospect in the field of starch synthesis of an industrial large system.

The present invention discloses α-glucan phosphorylase 5CC5 unit site mutant E535V and its preparation method and application. The present invention is aimed at the key enzyme elements of cellulose starch, which have low catalytic efficiency, poor stability and adaptability, and difficult heterologous expression. Through the big data analysis and bioinformatics methods of metagenomics and public databases, the novel cellulose degradation and starch synthesis key enzyme elements are accurately mined, and the α-glucan phosphorylase from tobacco is obtained by mining and identification, which has better enzyme activity and starch synthesis function than PGP from potato; the present invention further performs unit site mutation on the Cap domain of the α-glucan phosphorylase, and it is found that the unit site mutant obtained by performing E535V unit site mutation on the α-glucan phosphorylase from tobacco with an amino acid sequence as shown in SEQ ID No.1, and its enzyme activity and expression amount are significantly higher than those of wild-type α-glucan phosphorylase.

The application provides a domestication gene THERMO TOLERANCE 5 (TT5) for regulating heat tolerance of plants and application thereof. In the application, a temperature-dependent Oryza minuta chromosome substitution line material for regulating rice quality and seed setting is identified, a QTL capable of polyfunctionally protecting yield and quality under high temperature is located and cloned by a method of map-based cloning, and the QTL is TT5. TT5 is a positive regulation transcription factor for heat tolerance, polyfunctionally regulates starch synthesis and storage protein accumulation, and endows high temperature tolerance. Cloning of TT5 has important significance for guiding de novo domestication of wild rice, and rapid domestication of wild rice can be realized by adding or erasing methylation of a genome.

This invention discloses the application of the tobacco NtGBSS2 gene in regulating starch synthesis and / or drought resistance, and in improving tobacco germplasm resources. It belongs to the field of plant genetic engineering technology. The nucleotide sequence of the coding region of the tobacco NtGBSS2 gene is shown in SEQ ID NO.1. This invention overexpressed the NtGBSS2 gene in tobacco. Detection revealed that, compared to normal control tobacco plants, the content of resistant starch and amylose in the transgenic positive T1 generation was significantly increased. Furthermore, observation showed that wild-type control tobacco plants grew slowly and had yellowing leaves under drought stress, while the growth status of transgenic positive T2 generation tobacco plants was significantly improved compared to wild-type control tobacco plants, indicating that overexpression of the NtGBSS2 gene in tobacco enhances the tobacco's tolerance to drought stress.

The invention relates to an engineering flora for exocytosis synthesis of starch and a construction method and application thereof. The engineering bacteria comprise sucrose synthesis engineering bacteria and / or starch synthesis engineering bacteria, and the sucrose synthesis engineering bacteria over-express SUF1, SPP and SPS genes; the starch synthesis engineering bacteria overexpress at least one of AMS genes or BASPP and alpha GP genes. A brand new strategy for synthesizing starch by microorganisms is designed, a sucrose synthesis path is introduced and further strengthened to obtain the sucrose synthesis engineering bacteria, and a cheap and low-carbon raw material can be converted into sucrose; by introducing a starch synthesis path, a starch synthesis engineering bacterium is obtained, and cane sugar can be converted into extracellular starch. By utilizing double-bacterium synergy of sucrose synthesis engineering bacteria and starch synthesis engineering bacteria, extracellular secretion synthesis of starch is realized by utilizing a cheap carbon source in microorganisms, and a low-cost, safe and efficient starch production method is created.

The invention belongs to the technical field of biology, and discloses construction and application of an artificially synthesized starch recombinant strain. According to the invention, starch synthase is over-expressed in the bacterial cellulose high-yield strain, a competitive pathway of starch synthesis is blocked, and the bacterial cellulose high-yield strain is transformed into an artificial starch synthesis strain. Compared with an artificial starch synthesis path in the prior art, the method has the advantages of low cost, high starch yield, high chassis cell growth rate, sufficient starch synthesis precursor substances and the like.

The invention relates to application of a TaJAZ1 gene in wheat starch synthesis, and belongs to the field of gene engineering application. The nucleotide sequence of the TaJAZ1 gene is shown as SEQ ID NO.1, the TaJAZ1 gene has an improvement effect on the wheat starch quality, the thousand seed weight and the total starch content of wheat grains are increased, and the grains are full and full; the expansion potential, the solubility and the gelatinization temperature of the wheat grain starch are improved; on the molecular level, the TaJAZ1 gene participates in the synthesis of the wheat grain starch by controlling the expression of the TaAGPS1 gene and the TaAGPL1 gene.