102 results about "Starch synthesis" patented technology

Plants, particularly cereal plants, which have improved ability to synthesise starch at elevated or lowered temperatures and/or to synthesise starch with an altered fine structure are produced by inserting into the genome of the plant (i) a gene(s) encoding a form of an enzyme of the starch or glycogen biosynthetic pathway, particularly soluble starch synthase and/or branching enzyme and/or glycogen synthase, which display an activity which continues to increase over a temperature range over which the activity would normally be expected to decrease, and/or (ii) a gene(s) encoding sense and anti-sense constructs of enzymes of the starch biosynthetic pathway, particularly soluble starch synthase and/or branching enzyme and/or glycogen synthase, which alters the natural ratios of expression of the said enzymes or inserts enzymes with special structural characteristics which alter the natural branching pattern in starch.

Barley having a reduced level of SBEIIa activity produces grain having a high relative amylose content. The barley might additionally have reduced levels of SBEIIb activity. The barley grain of this invention can be of a non-shrunken phenotype despite a lesion in the amylopectin synthesis pathway.

Wheat having a reduced level of SBEIIa activity, that may have a relative high amylose content. Wheat having a mutant SBEIIa gene in the A genome. The wheat might additionally have reduced levels of SBEIIb activity. The wheat grain of this invention can be of a non-shrunken phenotype despite a lesion in the amylopectin synthesis pathway, and may also have a high relative amylose content.

The invention relates to a method for increasing the amylose content of plants. The invention provides enzymes, namely granule-bound starch synthase I (GBSS i), starch branching enzyme I (SBE I) and/or starch branching enzyme II (SBE II) which are applicable to genetic engineering operation in a starch synthesis path and are capable of remarkably changing starch composition of the plants. The invention also provides a novel method capable of favorably adjusting starch in plants, which comprises the following step of reducing the expression of the enzymes to obtain a series of transgenic plants which are different in amylose and amylopectin contents and proportions. The invention also provides a construct or carrier for adjusting the starch composition of plants.

The invention relates to nucleic acid molecules which code for enzymes and which are involved in the synthesis of starch in plants. These enzymes concern isoamylases derived from wheat. The invention also relates to vectors and host cells which contain the described nucleic acid molecules, especially transformed plant cells and plants which can be regenerated therefrom, which exhibit an increased or reduced activity of the inventive isoamylases.

The invention discloses a graphene three-dimensional printing material, and belongs to the technical field of materials for three-dimensional printing. The graphene three-dimensional printing material is prepared from the following raw materials in parts by weight: 5 to 8 parts of graphene, 26 to 32 parts of waste plastics, 32 to 42 parts of polylactic acid, 16 to 25 parts of sodium tripolyphosphate, 20 to 26 parts of n-butyl acetate, 12 to 20 parts of a specific synthesis agent and 5 to 10 parts of a starch synthesis agent. The graphene three-dimensional printing material prepared in the invention has the characteristics of high tensile strength, high bending strength and high notch impact strength, high heat deflection temperature and high melt index and the like, and the product printed through a three-dimensional printing technology is high in quality, high in impact and high in strength and has a broad market prospect.

The invention relates to the technical field of agriculture and in particular relates to a transgenic element and an application thereof, a method for differentiating a male sterility line and a fertile maintainer line, and an expanding propagation method of the male sterile line of maize. A functional nucleotide sequence controlling the male fertility of the maize and a nucleotide sequence controlling starch synthesis are transferred into the maize together, and then backcross transformation is performed to obtain a hybrid maintainer line. As the nucleotide sequence controlling starch synthesis in the maintainer line inhibits the starch synthesis and increase the sugar content to lead to kernel shrinking, the sterility line and the maintainer line can be doubly differentiated from quality and appearance, and the accuracy is improved. The male sterility line and the fertile maintainer line of the hybrid female parent of the maize can be differentiated accurately and efficiently by use of the method, and then the male sterility line of the hybrid female parent can be applied to expanding propagation and applied to the production of hybrid seeds. Meanwhile, the difference in quality and appearance is more prone to mechanical identification, and therefore, the working efficiency can be greatly improved.

The invention discloses a vegetable starch synthetase genetic promoter and use thereof. The promoter is separated from corn genome DNA with a nucleotide sequence which is showed as listed nucleotide sequence SEQ ID NO: 1; length of the nucleotide sequence is 1655bp. The vegetable starch synthetase genetic promoter of the invention can promote high expression of gene in leaf and seed without expression in root. The promoter is applicable to driving of gusA gene expression. The promoter plays an important role in vegetable starch quality improvement and vegetable biological reactor.

The invention discloses a method for regulating the content of starch in a corn kernel based on a ZmMIKC2a gene and belongs to the technical field of gene engineering. The method comprises the following steps: I) constructing a ZmMIKC2a gene over-expression vector to obtain a ZmMIKC2a gene over-expression plant; II) knocking out the ZmMIKC2a gene in a target corn to obtain a ZmMIKC2a gene deletion plant. The method disclosed by the invention has the advantages that the positive regulation effect of the ZmMIKC2a gene on corn kernel starch synthesis related genes is utilized; the content of amylose in the target corn and the ratio of the amylase to total starch are improved or reduced through a gene engineering method, so that a new transgenic corn variety with high amylose, low amylopectin or high amylopectin/low amylose is obtained; the method is of great practical significance in developing the new transgenic corn variety with a specific function by utilizing a gene engineering technology.

The invention belongs to the technical field of 3D printing material preparation and discloses a high-bending-strength rice straw 3D printing material. The high-bending-strength rice straw 3D printing material is prepared from, by weight, 72-116 parts of rice straw powder, 29-45 parts of polylactic acid, 10-14 parts of potassium hexaphoshpate, 11-15 parts of diethylene glycol butylether acetate, 9-12 parts of polypropylene resin, 3-5 parts of butadiene styrene rubber, 15-24 parts of a specific synthesis agent and 6-13 parts of a starch synthesis agent. The high-bending-strength rice straw 3D printing material is high in toughness and impact strength; products printed by means of 3D printing have advantages of high quality, high impact resistance, high strength, nontoxicity, low carbon, environment friendliness, low cost, wood color, recyclability, high economic value and promising market prospect.

The invention relates to a method of adjusting a content of plant amylose and amylopectin. The invention provides an enzyme which is suitable for genetic engineering operation and can substantially change starch composition in the plant, wherein the enzyme is granule-bound starch synthase I (GBSS I), starch branching enzyme I (SBE I) and/or starch branching enzyme II (SBE II). The invention further provides a novel method of well adjusting starch composition in the plant, and the method comprises reducing expression of the enzyme, and thus obtaining a series of transgene plants with different content ratios of amylose and amylopectin The invention further provides a constructor or a carrier for adjusting starch composition in the plant.

The present invention relates to a method of altering starch synthesis in a plant by modifying the starch priming activity of the plant. In particular, this is achieved by altering the expression or activity of a starch primer which is preferably encoded by the sequence of SEQ ID NO: 1 or a sequence substantially homologous thereto. Also provided are plants in which the starch priming activity has been altered and plant parts.

The invention discloses a plant expression vector and the application thereof in preparing phosphorylation modified rice starch. The plant expression vector comprises a target gene inserting into an original vector and a promoter, wherein the target gene comprises a transit peptides gene segment of granule-bound starch synthase and a potato glucan-water dikinase gene which are sequentially connected, and the promoter is a barley endosperm specific promoter HorD. For the plant expression vector and the application thereof in preparing phosphorylation modified rice starch, the potato glucan-water dikinase gene is transferred into rice, and the starch of the rice can be phosphorylated through the expression of the potato glucan-water dikinase gene, so that the modification of the starch is realized. The potato glucan-water dikinase is led to amyloid through the transit peptides of the granule-bound starch synthase, and phosphorylation modification is performed on the starch. According to the invention, the expression of the potato glucan-water dikinase gene in the rice endosperm can be promoted through the barley endosperm specific promoter HorD, and the phosphorus content in the rice starch is greatly increased.

The present invention relates to a novel preparation method of starch octenyl succinate, the main technical point of which is to carry out hot alkali pretreatment on starch, and then adopt two methods of direct modification or modification after freeze-drying and then carry out octenyl Synthesis of starch octenyl succinate to improve the emulsification performance of the final product starch octenyl succinate. The invention has simple operation steps and low cost, and the octenyl succinic acid starch ester treated with hot alkali freeze-intervention has a higher degree of substitution and better emulsifying performance than the starch ester without pretreatment.

The invention discloses a method for regulating starch contents of rice grains based on a ZmMIKC2a gene. The ZmMIKC2a gene is a DNA molecule, of which the nucleotide sequence is shown in SEQ ID NO.1. The method comprises the following steps: building an excessive expression carrier of the ZmMIKC2a gene, and importing the excessive expression carrier into a target paddy rice genome to obtain an excessive expression plant of the ZmMIKC2a gene of which straight-chain starch content and the proportion of the straight-chain starch to total starch in seed grains are both higher than that of the target paddy rice. According to the method, positive regulation effects of the ZmMIKC2a gene on a plant seed grain starch synthesis related gene is used, by adopting a genetic engineering method, the straight-chain starch content and the proportion of the straight-chain starch to the total starch in the paddy rice seed grains are changed, so that a new variety of genetically modified paddy rice with high straight-chain starch is obtained, and important practical meaning on development of the new variety of genetically modified paddy rice with a specific function by utilizing a genetic engineering technology is achieved.

The invention discloses a plant starch synthesized related protein IbSSI, a coded gene and application thereof. The plant starch synthesized related protein IbSSI provided by the invention is 1) or 2) or 3): 1) the protein with an amino acid sequence shown as sequence 2 of a sequence table; 2) fusion protein obtained by connecting a label to the N or/and C end of the protein shown as the sequence 2 of the sequence table; and 3) the plant starch synthesis related protein obtained by subjecting the protein shown as 1) or 2) to substitution and/or deletion and/or adding by one or more amino acid residues. Experiments prove that the plant starch synthesized related protein IbSSI and the coded gene thereof provided by the invention can increase the total content of plant starch and/or change the starch quality.