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85 results about "Glycome" patented technology

The glycome is the entire complement of sugars, whether free or present in more complex molecules, of an organism. An alternative definition is the entirety of carbohydrates in a cell. The glycome may in fact be one of the most complex entities in nature. "Glycomics, analogous to genomics and proteomics, is the systematic study of all glycan structures of a given cell type or organism" and is a subset of glycobiology.

Three dimensional vaginal tissue model containing immune cells

Disclosed is a cervico-vaginal tissue equivalent comprised of vaginal epithelial cells and immune cells, cultured at the air-liquid interface. The tissue equivalent is capable of being infected with a sexually transmitted pathogen such as a virus (e.g., HIV), a bacteria, a helminthic parasite, or a fungus. The tissue equivalent is also capable of undergoing an allergic-type reaction or an irritant-type reaction. The tissue equivalent is characterized as having nucleated basal layer cells and nucleated suprabasal layer cells, and further as having cell layers external to the suprabasal layer progressively increasing in glycogen content and progressively decreasing in nuclei content. Immune cells of the tissue equivalent are primarily located in the basal and suprabasal layers. Also disclosed are methods for producing the tissue equivalent. The methods involve providing vaginal epithelial cells and immune cells, seeding the cells onto a porous support, and co culturing the seeded cells at the air-liquid interface under conditions appropriate for differentiation. One such method disclosed is for generation of the tissue equivalent in serum free medium. Specific cells from which the tissue equivalent is generated, and also specific preferred components of the medium in which the tissue equivalent is generated are provided. Also disclosed is a cervico-vaginal tissue equivalent produced by the methods disclosed herein.
Owner:MATTEK CORP

Extraction method of apostichopus japonicus body-wall total RNA

InactiveCN101864414AMeet Gene Expression AnalysisFulfil requirementsDNA preparationWater bathsTotal rna
The invention discloses a high-extraction-purity, good-integrity and high-yield extraction method of apostichopus japonicus body-wall total RNA (Ribonucleic Acid). The method comprises the following steps of: quickly freezing apostichopus japonicus body-wall tissue in liquid nitrogen; grinding and putting the frozen tissue in lysate to homogenate, centrifugate and take supernatant fluid; adding chloroform to the supernatant fluid and centrifugating to take the supernatant fluid to another centrifuge tube; then, adding a high-salt solution and isopropyl alcohol and filtering precipitation with ethanol; dissolving the precipitation with DEPC (diethypyrocarbonate) treated water to have constant volume; sequentially adding a DNA enzyme buffer solution without RNA enzymes, DNA enzymes without RNA enzymes and an RNA enzyme inhibitor to the dissolved solution to mix; carrying out a water bath at 37 DEG C to obtain DNA lysate; adding phenol and chloroform in a ratio of 5:1 to the DNA lysate to mix, centrifugate and take supernatant fluid; adding a glycogen solution, a potassium acetate solution and pre-cooling ethanol to the supernatant fluid; mixing and staying over night; centrifugating to discard the supernatant fluid; washing and dying the precipitation with ethanol; dissolving the solution with the DEPC treated water to have constant volume of 20 mu l; and storing at 80 DEG C below zero.
Owner:DALIAN OCEAN UNIV

Method for establishing seroglycoid N-glycome atlas model of chronic hepatitis liver injury

The invention discloses a method for establishing a seroglycoid N-glycome atlas model of chronic hepatitis liver injury. The method comprises the following steps: detecting a seroglycoid N-glycome atlas by using a G-Test detection method, establishing an N-glycome atlas model with significant difference between liver injury patients and normal control personnel, and screening NG1A2F with significant expression difference between a liver injury group and a normal control group. Based on the N-glycome atlas model established by the method disclosed by the invention, the detection sensitivity andspecificity of liver injury respectively reach 80.0% and 74.4%. In subsequent applications, the degree of liver injury of personnel to be detected can be detected by comparing the peak values of single peak NG1A2F in the N-glycome atlas of the serum of the personnel to be detected and in the atlas model established by the method. Based on the method disclosed by the invention, a plurality of patients with chronic hepatitis liver injury can be subjected to routine and non-invasive detection to help doctors and the patients to monitor the occurrence and progression of the chronic hepatitis liver injury in time, and the method is expected to be promoted and used in clinical practice.
Owner:JIANGSU XIANSIDA BIOTECH CO LTD +1

Separation and purification method of human umbilical cord mesenchymal stem cell exosome and application of human umbilical cord mesenchymal stem cell exosome

The invention provides a separation and purification method of a human umbilical cord mesenchymal stem cell exosome and an application of the human umbilical cord mesenchymal stem cell exosome, and belongs to the technical field of medicines. The human umbilical cord mesenchymal stem cell exosome provided by the invention is prepared by cultivating human umbilical cord mesenchymal stem cells via aserum-free medium, collecting supernatant liquid, implementing centrifuging as well as ultra-filtration and concentration, transferring a concentrated solution onto a 30% sucrose-heavy water densitypad and implementing further purification through sucrose density centrifuging, so that the human umbilical cord mesenchymal stem cell exosome is obtained. According to the separation and purificationmethod that the human umbilical cord mesenchymal stem cell exosome is obtained through separation and purification, immunoreactivity is effectively reduced, and a controllable dosage when the human umbilical cord mesenchymal stem cell exosome is used is guaranteed. The human umbilical cord mesenchymal stem cell exosome, by improving a degree of activating an insulin signaling pathway of a type IIdiabetes animal model, can inhibit composition and decomposition of hepatic glycogen, so that glucose metabolism homeostasis can be achieved; and meanwhile, the sensitivity of the type II diabetes animal model to insulin and an insulin secretion function of pancreatic [beta] cells can be improved and a blood glucose concentration can be reduced, so that the application of the human umbilical cordmesenchymal stem cell exosome to the preparation of medicines for treating type II diabetes can be achieved.
Owner:JIANGSU UNIV

Hepatic failure serum glycoprotein N-glycome map model establishing method

The invention discloses a hepatic failure serum glycoprotein N-glycome map model establishing method, wherein a serum glycoprotein N-glycome map is detected by using a G-Test detection method, the N-glycome map model having significant difference between hepatic failure patients and normal control people is established, and the NA2 having significant expression difference between the hepatic failure group and the normal control group is screened. According to the present invention, the hepatic failure detection sensitivity and the hepatic failure detection specificity of the N-glycome map model constructed based on the method of the present invention respectively are 100.0% and 97.6%; in the subsequent applications, by comparing the peak value of the single-peak NA2 in the serum glycoprotein N-glycome map of the person to be detected and in the map model established by the method of the present invention, the hepatic failure degree of the person to be detected can be detected; and baseon the method of the present invention, a large number of hepatic failure patients can be subjected to conventional and non-invasive detection so as to help doctors and patients timely monitor the occurrence and the progression of hepatic failure, such that the method is expected to be popularized in clinical practice.
Owner:JIANGSU XIANSIDA BIOTECH CO LTD +1

Reagent composition for separating total RNA in plant or microorganism and preparation method thereof

The invention belongs to the field of molecular biology method and relates to a reagent composition for separating total RNA in a plant or a microorganism and a preparation method thereof. The high quality RNA can be obtained after a simple extraction by chloroform while guanidinium isothiocyanate and phenol are regarded as main components, positive ions are provided by NaCl, MgCl2 and the like, and a solution pH is stabilized by a sodium acetate-acetic acid buffer system. Glycogen serving as a nucleic acid precipitant is added in the RNA extraction reagent in the invention, so that the reagent disclosed by the invention, in comparison with reagents of the same type, greatly improves precipitation efficiency of the nucleic acid and also can efficiently separate the nucleic acid component which has a very low content in a tissue sample. Therefore, the nucleic acid precipitation process can be finished in a short period, the precipitation process at -20 DEG C for hours is avoided, and operating time is shortened greatly. The method is rapid as well as efficient and has wide applicable samples; and the reagent composition disclosed by the invention is cheaper than commercial TRIzol reagents. The disadvantages of high sample selectivity, fussy operation and relatively low efficiency of the traditional method are overcome. The operation is more flexible; and the sample after being homogenized can be stored at -20 DEG C and then used for the RNA extraction.
Owner:HUAZHONG AGRI UNIV
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