Separation and purification method of human umbilical cord mesenchymal stem cell exosome and application of human umbilical cord mesenchymal stem cell exosome

Abstract

The invention provides a separation and purification method of a human umbilical cord mesenchymal stem cell exosome and an application of the human umbilical cord mesenchymal stem cell exosome, and belongs to the technical field of medicines. The human umbilical cord mesenchymal stem cell exosome provided by the invention is prepared by cultivating human umbilical cord mesenchymal stem cells via aserum-free medium, collecting supernatant liquid, implementing centrifuging as well as ultra-filtration and concentration, transferring a concentrated solution onto a 30% sucrose-heavy water densitypad and implementing further purification through sucrose density centrifuging, so that the human umbilical cord mesenchymal stem cell exosome is obtained. According to the separation and purificationmethod that the human umbilical cord mesenchymal stem cell exosome is obtained through separation and purification, immunoreactivity is effectively reduced, and a controllable dosage when the human umbilical cord mesenchymal stem cell exosome is used is guaranteed. The human umbilical cord mesenchymal stem cell exosome, by improving a degree of activating an insulin signaling pathway of a type IIdiabetes animal model, can inhibit composition and decomposition of hepatic glycogen, so that glucose metabolism homeostasis can be achieved; and meanwhile, the sensitivity of the type II diabetes animal model to insulin and an insulin secretion function of pancreatic [beta] cells can be improved and a blood glucose concentration can be reduced, so that the application of the human umbilical cordmesenchymal stem cell exosome to the preparation of medicines for treating type II diabetes can be achieved.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a method for separating and purifying human umbilical cord mesenchymal stem cell exosomes and the application of human umbilical cord mesenchymal stem cell exosomes. Background technique [0002] Diabetes mellitus is a metabolic disease characterized by hyperglycemia due to defects in insulin secretion or insulin action. In the past 30 years, the incidence of diabetes in China has increased from less than 1% to more than 10%. The number of diabetic patients has exceeded 100 million, ranking first in the world, and they are getting younger. Diabetes has become a serious public health problem in my country. 90% to 95% of diabetic patients are type 2 diabetes mellitus (T2DM). Patients with type 2 diabetes mellitus regulate their blood sugar through diet control, exercise and oral hypoglycemic drugs in the early stage. With the development of the disease, most patients ...

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Problems solved by technology

[0003] Many studies at home and abroad have shown that mesenchymal stem cells (MSCs) play an important role in blood sugar regulation and islet cell function repair. MSCs can improve the sensitivity of peripheral tissues to insulin and increase the use of glucose in blood by tissues. , has shown certain effects in the intervention of diabetes, but the application standards of MSC, immune reactivity, potential tumorigenicity and the safety of transgenic technology limit the application of MSC in the treatment of diabetes

At present, MSC has many toxic and side effects in the treatment of diabetes, and it is only used to control the disease without substantial improvement in the etiology; there are also ethical, uncontrollable dosage and safety issues

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