9results about How to "Increase acquisition rate" patented technology

The invention relates to a genotype-dependence-free dendrobium nobile genetic transformation method and application, and belongs to the technical field of genetic engineering. Comprising the following steps: taking embryonic calluses, protocorms or protocorm-like bodies of dendrobium nobile as explants; introducing the binary vector carrying the target gene into agrobacterium to prepare an infection solution, and infecting the explant; after co-culture, transferring to a culture medium containing a screening agent to obtain a resistant explant; and carrying out differentiation and rooting culture to obtain a transgenic plant. By systematically optimizing a plant growth regulator combination, an agrobacterium strain type, an infection mode and an auxiliary screening mode, an efficient and stable genetic transformation system which does not depend on a specific genotype and takes multiple types of explants as receptors is established for the first time, and the problems that a traditional method is high in genotype dependency and limited in tissue type are solved; the method shows good applicability and repeatability in a plurality of dendrobium hybrids, and provides a general technical platform for gene function research and molecular breeding.

The invention discloses an eurytopic chlamydomonas reinhardtii expression vector based on a PSAD promoter and a 3 * Flag tag as well as a construction method and application of the eurytopic chlamydomonas reinhardtii expression vector. The nucleotide sequence of the eurytopic chlamydomonas reinhardtii expression vector is shown as SEQ ID No.1; the vector comprises a PSAD promoter, a target gene insertion site EcoR V, a 3 * Flag tag at the 3'terminal, an rbcS2 terminator, a TUB2 promoter, a hygromycin B screening gene and an rbcS2 terminator. The construction method comprises the following steps: constructing a skeleton of a pHyg-PSAD-3Flag vector; constructing a sequence fragment fused with a 3 * Flag tag; and connecting the sequence fragment fused with the 3 * Flag tag with a plasmid skeleton fragment through T4 DNA (deoxyribonucleic acid) enzyme so as to construct the pHyg-PSAD-3Flag vector. According to the invention, the expression plasmid of the chlamydomonas pHyg-PSAD-3Flag can be used for efficiently screening algae strains capable of stably expressing target protein.

This invention discloses the application of N-acetyl-L-tryptophan, including the following applications: a. application in the preparation of drugs or health products that improve ovarian reserve function; b. application in the preparation of drugs or health products that improve or treat oocyte maturation disorders. This invention also discloses a drug comprising: N-acetyl-L-tryptophan or a pharmaceutically acceptable salt thereof, a pharmaceutically acceptable carrier, or an excipient; said drug can improve or treat oocyte maturation disorders and improve ovarian reserve function. This invention also discloses a health product comprising: N-acetyl-L-tryptophan or a pharmaceutically acceptable salt thereof, a pharmaceutically acceptable carrier, or an excipient; said health product can improve or treat oocyte maturation disorders and improve ovarian reserve function. This invention is the first to discover that N-acetyl-L-tryptophan has the efficacy of improving or treating oocyte maturation disorders and improving ovarian reserve function.

The present application relates to the technical field of mineral material microcharacterization, and in particular to a method for dispersing clay mineral monolayer suitable for transmission electron microscope observation. The method for dispersing clay mineral monolayer based on stage alcohol intercalation and exfoliation comprises the following steps: dispersing clay mineral in deionized water to obtain a clay mineral suspension system by stirring; adding small molecule alcohol to the suspension system first and then adding macromolecular alcohol, and stirring to obtain a dilute dispersion system; performing ultrasonic treatment to make the clay mineral form a dispersion system mainly in monolayer or few-layer structure; dropping onto a carrier film for transmission electron microscope to make the lamella spread on the surface of the carrier film; and observing by using a transmission electron microscope. The present application is conducive to obtaining clay mineral monolayer samples with good dispersion and complete structure, and is suitable for morphology and structure characterization under transmission electron microscope.

The application discloses a culture method for propagating Brachythecium populeum, and belongs to the technical field of Brachythecium populeum tissue culture. The application uses sporangium as an explant to propagate Brachythecium populeum, and quickly obtains Brachythecium populeum spores with a high germination rate, establishes a regeneration system of Brachythecium populeum, lays a foundation for large-scale propagation of Brachythecium populeum, shortens the propagation cycle of Brachythecium populeum, and better maintains excellent properties. The application first establishes a method for culturing Brachythecium populeum by using spores, fills the blank of the prior art, and provides a technical basis for propagation, seed preservation, preparation of protoplasts, recombination experiments and gametophyte culture of Brachythecium populeum.

This invention relates to the field of yak milk processing technology, and discloses a device and method for separating active substances from yak milk, comprising: a first separation unit, a first processing unit, a pH adjustment unit, a second separation unit, and a third separation unit connected in sequence; the first separation unit is used for centrifugation of yak milk to obtain skimmed yak milk; the first processing unit is used for defoaming the skimmed yak milk; the second separation unit is used for centrifugation of the pH-adjusted skimmed yak milk to obtain supernatant and casein; the third separation unit is used for chromatographic separation of the supernatant to obtain lactoferrin and immunoglobulins. The defoaming treatment of the skimmed yak milk in the first processing unit effectively eliminates air bubbles in the skimmed milk, preventing air bubbles from occupying space and hindering the full contact between the acid solution and the skimmed yak milk, preventing local pH unevenness, improving the mixing effect of the skimmed yak milk and acid solution, increasing the casein yield, and ensuring the subsequent separation effect.

The invention particularly relates to a human pneumosarcoma-like carcinoma organ as well as a construction method and application thereof, and belongs to the technical field of biological medicines. The organoid is selected from TJLCI-PSC-O-1 with the preservation number of CCTCC (China Center For Type Culture Collection) NO: C2025218 or TJLCI-PSC-O-2 with the preservation number of CCTCC NO: C2025334, or an amplified culture of an epithelial marker CK and a mesenchymal marker VIMENTIN which are stably co-expressed by the organoid. According to the construction method, a collagenase / dispersing enzyme and DNase I combined stepped enzymolysis system and a three-dimensional culture system are adopted, and high yield and long-term stable amplification of cells are realized. The model highly simulates the pulmonary sarcomatoid carcinoma pathological structure and epithelial-mesenchymal transition phenotype for the first time, can be used for in-vitro drug sensitivity test, in-vivo tumor formation model construction and drug screening, and provides a key platform for basic research and precise treatment of diseases.

The application belongs to the technical field of soybean phospholipid, and particularly relates to an enzymatic soybean lecithin with whitening and anti-aging effects, a preparation method and application thereof. The preparation method comprises the following steps: (1) taking soybean powder phospholipid, adding solution A, stirring, centrifuging, and obtaining a precipitate; (2) adding anhydrous ethanol to the precipitate, standing, concentrating the supernatant, and obtaining a concentrated solution; (3) adding a composite enzyme and a buffer solution to the concentrated solution for enzymolysis, drying, and obtaining crude phospholipid; (4) eluting the crude phospholipid through a silica gel-aluminum oxide column, collecting and concentrating the eluent, and drying, to obtain the enzymatic soybean lecithin; wherein the solution A in step (1) is a mixed solution of acetone, methanol and a citric acid aqueous solution. The prepared enzymatic soybean lecithin has high purity, good activity and high hydrophilicity, and has significant whitening and anti-aging effects.

This invention relates to the field of geological exploration coring equipment technology, specifically to a coring device and method for shale gas and tight sandstone gas exploration. The coring device includes an outer tube, an inner tube, a reduced-diameter sleeve, a clamp-type core claw, a guide cylinder, a guide rod, a core break cutter, and a linkage assembly. The reduced-diameter sleeve is located at the bottom of the inner tube and inside the outer tube; the clamp-type core claw is installed on the reduced-diameter surface; the guide cylinder is inclined upwards and mounted on the reduced-diameter sleeve; the guide rod passes through the guide cylinder; the core break cutter is located at the bottom of the guide rod; when the clamp-type core claw moves downwards, it drives multiple sets of guide rods to move inwards synchronously through the linkage assembly. The multiple sets of guide rods and the core break cutter of this invention form a bottoming structure inwards, which supports the core inside the inner tube, enabling the relatively complete extraction of cores from interlayered shale reservoirs and tight sandstone reservoirs.