20results about How to "Increase acquisition rate" patented technology

The invention relates to the technical field of radio communication, and discloses an XL-RIS-assisted near-field multi-beam design method and system, and the method comprises the steps: constructing an XL-RIS-assisted near-field communication multi-user receiving signal model; constructing a near-field multi-beam optimization model; the near-field multi-beam optimization model is solved, so that an XL-RIS beam reflection matrix can be obtained; and iteratively solving the near-field multi-beam optimization model to obtain a reflection matrix of a base station transmitting signal to the XL-RIS, and completing XL-RIS assisted system multi-beam optimization. Compared with the existing calculation, the beam optimization method provided by the invention does not need a complicated calculation problem, overcomes the problems of low convergence speed and high calculation complexity in large-scale array processing of the traditional method, and improves the optimization efficiency. The operation speed can be increased under the condition that the system performance is guaranteed.

The invention relates to the technical field of rubber tree seedling cultivation, and particularly discloses a cultivation method for efficiently obtaining a normal somatic embryo of a rubber tree clone variety, and the cultivation method comprises the following steps: S1, taking an anther newborn somatic cell immature embryo of the rubber tree clone variety, and picking out a cotyledon small embryo; s2, placing the cotyledon small embryos in an improved MS culture medium for maturation culture to obtain mature normal somatic embryos; s3, transferring the mature normal somatic embryo into a somatic embryo germination culture medium for somatic embryo plant induction culture. The method for cultivating the normal somatic embryo of the rubber tree clone variety has better maturation culture efficiency and higher normal somatic embryo and somatic embryo plant obtaining rate, and large-scale propagation can be realized. The maturing rate of cotyledon small embryos reaches up to 82.50% or above, the obtaining rate of normal somatic embryos reaches up to 75.0% or above, and the obtaining rate of somatic embryo plants reaches up to 63.0% or above.

The invention relates to the technical field of adaptive control, in particular to an adaptive control system of movable intelligent sampling equipment based on reinforcement learning. The system comprises a state and action construction unit, a reinforcement learning construction unit and a self-adaptive control unit, the state and action construction unit is used for performing superposition modeling on a plurality of concentration sources of a target substance to obtain a concentration field of the target substance, calculating a concentration field gradient, obtaining a real-time state of equipment, and constructing a state by taking the real-time state of the equipment, the concentration field and the concentration field gradient as elements in a state space; defining an action; the reinforcement learning construction unit is used for constructing a reward function of reinforcement learning according to the state and the action so as to generate a reinforcement learning model; and the adaptive control unit is used for carrying out strategy optimization, generating a control track and carrying out sampling quality evaluation according to the reinforcement learning model. According to the invention, autonomous decision making and efficient sampling of equipment in a dynamic concentration field are realized.

The present invention discloses a directional horizontal hole advance exploration coal mine fault coring device and a method for preventing core slippage, comprising an outer cylinder and an inner cylinder coaxially arranged in the outer cylinder, a coring drill bit coaxially installed on the front end of the outer cylinder, a core claw assembly coaxially installed on the front end of the inner cylinder, the core claw assembly is located in the coring drill bit, and the distance from the front end face of the core claw assembly to the inner shoulder surface of the coring drill bit is greater than zero; a roller bearing is installed between the core claw assembly and the coring drill bit, and a rotating assembly component is coaxially installed in the rear end of the outer cylinder. The present invention can ensure high core integrity, not easy to break, and high core success rate during horizontal coring; by setting the front end of the core claw seat as a conical hole, the outer wall of the core can be squeezed and strengthened to ensure the integrity of the coring and avoid the core from spreading, and the friction between the core claw seat and the core can be increased when pulling the drill.

The invention relates to a genotype-dependence-free dendrobium nobile genetic transformation method and application, and belongs to the technical field of genetic engineering. Comprising the following steps: taking embryonic calluses, protocorms or protocorm-like bodies of dendrobium nobile as explants; introducing the binary vector carrying the target gene into agrobacterium to prepare an infection solution, and infecting the explant; after co-culture, transferring to a culture medium containing a screening agent to obtain a resistant explant; and carrying out differentiation and rooting culture to obtain a transgenic plant. By systematically optimizing a plant growth regulator combination, an agrobacterium strain type, an infection mode and an auxiliary screening mode, an efficient and stable genetic transformation system which does not depend on a specific genotype and takes multiple types of explants as receptors is established for the first time, and the problems that a traditional method is high in genotype dependency and limited in tissue type are solved; the method shows good applicability and repeatability in a plurality of dendrobium hybrids, and provides a general technical platform for gene function research and molecular breeding.

The invention discloses an eurytopic chlamydomonas reinhardtii expression vector based on a PSAD promoter and a 3 * Flag tag as well as a construction method and application of the eurytopic chlamydomonas reinhardtii expression vector. The nucleotide sequence of the eurytopic chlamydomonas reinhardtii expression vector is shown as SEQ ID No.1; the vector comprises a PSAD promoter, a target gene insertion site EcoR V, a 3 * Flag tag at the 3'terminal, an rbcS2 terminator, a TUB2 promoter, a hygromycin B screening gene and an rbcS2 terminator. The construction method comprises the following steps: constructing a skeleton of a pHyg-PSAD-3Flag vector; constructing a sequence fragment fused with a 3 * Flag tag; and connecting the sequence fragment fused with the 3 * Flag tag with a plasmid skeleton fragment through T4 DNA (deoxyribonucleic acid) enzyme so as to construct the pHyg-PSAD-3Flag vector. According to the invention, the expression plasmid of the chlamydomonas pHyg-PSAD-3Flag can be used for efficiently screening algae strains capable of stably expressing target protein.

The present invention provides a bovine oocyte maturation fluid and application, belonging to the field of animal breeding technology. The present invention effectively improves the bovine oocyte maturation efficiency, promotes the proliferation of cumulus cells, improves the embryo development ability, and thus improves the bovine in vitro embryo production efficiency by adding lipoxin to the basic culture medium.

This invention discloses the application of N-acetyl-L-tryptophan, including the following applications: a. application in the preparation of drugs or health products that improve ovarian reserve function; b. application in the preparation of drugs or health products that improve or treat oocyte maturation disorders. This invention also discloses a drug comprising: N-acetyl-L-tryptophan or a pharmaceutically acceptable salt thereof, a pharmaceutically acceptable carrier, or an excipient; said drug can improve or treat oocyte maturation disorders and improve ovarian reserve function. This invention also discloses a health product comprising: N-acetyl-L-tryptophan or a pharmaceutically acceptable salt thereof, a pharmaceutically acceptable carrier, or an excipient; said health product can improve or treat oocyte maturation disorders and improve ovarian reserve function. This invention is the first to discover that N-acetyl-L-tryptophan has the efficacy of improving or treating oocyte maturation disorders and improving ovarian reserve function.

The present application relates to the technical field of mineral material microcharacterization, and in particular to a method for dispersing clay mineral monolayer suitable for transmission electron microscope observation. The method for dispersing clay mineral monolayer based on stage alcohol intercalation and exfoliation comprises the following steps: dispersing clay mineral in deionized water to obtain a clay mineral suspension system by stirring; adding small molecule alcohol to the suspension system first and then adding macromolecular alcohol, and stirring to obtain a dilute dispersion system; performing ultrasonic treatment to make the clay mineral form a dispersion system mainly in monolayer or few-layer structure; dropping onto a carrier film for transmission electron microscope to make the lamella spread on the surface of the carrier film; and observing by using a transmission electron microscope. The present application is conducive to obtaining clay mineral monolayer samples with good dispersion and complete structure, and is suitable for morphology and structure characterization under transmission electron microscope.

The invention belongs to a 2D wood cell wall thin layer batch preparation method which comprises the following steps: sequentially treating wood powder with an alkaline solution and a sodium hypochlorite solution, then putting the wood powder in a wet state into a kneading machine, carrying out mechanical shear mixing treatment for 4-5 hours, adding low-amount cellulase during the period, and promoting cell wall separation by cooperating with the mechanical shear mixing effect, so as to obtain the 2D wood cell wall thin layer. The 2D wood cell wall thin layer batch preparation material is obtained. According to the method, the 2D wood cell wall thin layer material can be obtained through high-yield, high-efficiency, low-cost and industrial-grade expansion, TEMPO treatment and ultrasonic treatment which are high in energy consumption and have environmental burden are not included in the method, large-scale preparation of the material is achieved through the kneading technology, the stripping yield of the 2D cell wall thin layer can be greatly increased, and the method is suitable for large-scale production of the 2D wood cell wall thin layer material. The stripping yield of the 2D cell wall thin layer can reach about 75%, the obtaining rate is high, and the requirement of large-scale application can be met.

The invention belongs to the technical field of regenerative medicine and biology, and particularly relates to an amplification and purification culture method of umbilical cord multi-line differentiation stress tolerance cells. The method comprises the steps of umbilical cord stem cell primary culture, umbilical cord stem cell subculture and umbilical cord Muse cell inoculated culture. Compared with the prior art, the method has the advantages that the totipotent type of Muse cells is well stabilized and maintained by creatively adding a certain amount of the totipotent stem cell culture medium through a Muse culture formula; meanwhile, substances such as NEAA are added, the Muse cells are efficiently and accurately subjected to in-vitro amplification, and the method is simple in operation step, high in cell acquisition rate, sufficient in number of extracted cells and high in cell viability. The experiment operation time is short, the obtained cell viability is high, and the cell number is greatly increased.

The invention provides a method for separating cells from bone marrow and inducing to generate endothelial progenitor cells, which comprises the following steps of: firstly, killing an SD (Sprague Dawley) rat by using a cervical vertebra dislocation method, and disinfecting the surface of the rat by using 75% ethanol; thirdly, cutting open the lower limbs of the rat to take out the femur and the tibia, and cutting off two ends of the bone to expose a marrow cavity; then, preparing an ECM culture medium containing 5% of fetal calf serum, 1% of ECGS and 1% of PS, and flushing the marrow cavity with the ECM culture medium until the bone becomes white; then, collecting the flushing fluid, centrifuging to remove supernate, and filtering by using a 200-mesh cell sieve to remove impurities; then, inoculating the cell precipitate into a six-well plate, and putting the six-well plate into a constant-temperature incubator with the temperature of 37 DEG C and the CO2 content of 5% for culturing; after 72 hours, changing the liquid to remove non-adherent cells, and then changing the liquid once every two days until the cells need passage; and finally, when the fusion degree of the cells reaches about 80%, digesting the cells by using 0.25% trypsin, and inoculating the cells into a T25 culture bottle for further culture. The method is simple to operate, and the cell purity and the differentiation efficiency of the endothelial progenitor cells are effectively improved.

The invention provides an antibody conjugate (smacAb) and application of the antibody conjugate (smacAb). The invention also provides a construction method of the smacAb library and a screening method of the smacAb. The smacAb prepared by the invention has higher affinity, inhibitory activity and specificity, and the constructed smacAb library has diversity and a library capacity of about 109.

The application discloses a culture method for propagating Brachythecium populeum, and belongs to the technical field of Brachythecium populeum tissue culture. The application uses sporangium as an explant to propagate Brachythecium populeum, and quickly obtains Brachythecium populeum spores with a high germination rate, establishes a regeneration system of Brachythecium populeum, lays a foundation for large-scale propagation of Brachythecium populeum, shortens the propagation cycle of Brachythecium populeum, and better maintains excellent properties. The application first establishes a method for culturing Brachythecium populeum by using spores, fills the blank of the prior art, and provides a technical basis for propagation, seed preservation, preparation of protoplasts, recombination experiments and gametophyte culture of Brachythecium populeum.

This invention relates to the field of yak milk processing technology, and discloses a device and method for separating active substances from yak milk, comprising: a first separation unit, a first processing unit, a pH adjustment unit, a second separation unit, and a third separation unit connected in sequence; the first separation unit is used for centrifugation of yak milk to obtain skimmed yak milk; the first processing unit is used for defoaming the skimmed yak milk; the second separation unit is used for centrifugation of the pH-adjusted skimmed yak milk to obtain supernatant and casein; the third separation unit is used for chromatographic separation of the supernatant to obtain lactoferrin and immunoglobulins. The defoaming treatment of the skimmed yak milk in the first processing unit effectively eliminates air bubbles in the skimmed milk, preventing air bubbles from occupying space and hindering the full contact between the acid solution and the skimmed yak milk, preventing local pH unevenness, improving the mixing effect of the skimmed yak milk and acid solution, increasing the casein yield, and ensuring the subsequent separation effect.

The present invention discloses a method, device and storage medium for leaching ionic rare earth ores based on digital twins, which relates to the technical field of mine leaching and can improve the leaching rate and leaching efficiency of rare earth elements. It includes: constructing a preset digital twin coupling model of rare earth ores, including a preset ionic rare earth element leaching model and a preset hydrometallurgical treatment model; obtaining the leaching agent property information of the leaching agent, the rare earth ore property information corresponding to the target ionic rare earth ore, and the operating parameters under the in-situ leaching process; inputting the leaching agent property information, the rare earth ore property information and the operating parameters into the preset ionic rare earth element leaching model to obtain the overall seepage field distribution data and concentration field distribution data of the rare earth mine; inputting the extracted seepage field distribution data and concentration field distribution data at the liquid collection tunnel as initial parameters into the preset hydrometallurgical treatment model to obtain the predicted process operation parameter information of the hydrometallurgical treatment process; based on the predicted process operation parameter information, hydrometallurgical treatment is performed on the leaching mother liquor to obtain ionic rare earth elements.

The invention particularly relates to a human pneumosarcoma-like carcinoma organ as well as a construction method and application thereof, and belongs to the technical field of biological medicines. The organoid is selected from TJLCI-PSC-O-1 with the preservation number of CCTCC (China Center For Type Culture Collection) NO: C2025218 or TJLCI-PSC-O-2 with the preservation number of CCTCC NO: C2025334, or an amplified culture of an epithelial marker CK and a mesenchymal marker VIMENTIN which are stably co-expressed by the organoid. According to the construction method, a collagenase / dispersing enzyme and DNase I combined stepped enzymolysis system and a three-dimensional culture system are adopted, and high yield and long-term stable amplification of cells are realized. The model highly simulates the pulmonary sarcomatoid carcinoma pathological structure and epithelial-mesenchymal transition phenotype for the first time, can be used for in-vitro drug sensitivity test, in-vivo tumor formation model construction and drug screening, and provides a key platform for basic research and precise treatment of diseases.

The application belongs to the technical field of soybean phospholipid, and particularly relates to an enzymatic soybean lecithin with whitening and anti-aging effects, a preparation method and application thereof. The preparation method comprises the following steps: (1) taking soybean powder phospholipid, adding solution A, stirring, centrifuging, and obtaining a precipitate; (2) adding anhydrous ethanol to the precipitate, standing, concentrating the supernatant, and obtaining a concentrated solution; (3) adding a composite enzyme and a buffer solution to the concentrated solution for enzymolysis, drying, and obtaining crude phospholipid; (4) eluting the crude phospholipid through a silica gel-aluminum oxide column, collecting and concentrating the eluent, and drying, to obtain the enzymatic soybean lecithin; wherein the solution A in step (1) is a mixed solution of acetone, methanol and a citric acid aqueous solution. The prepared enzymatic soybean lecithin has high purity, good activity and high hydrophilicity, and has significant whitening and anti-aging effects.

The invention relates to a method for one-step in-situ preparation of a Z-type NiMn2O4 / NiO composite photocatalyst with a compact interface and application of the Z-type NiMn2O4 / NiO composite photocatalyst. The preparation method comprises the following steps: firstly, respectively dispersing MnCl2. 4H2O and Ni (NO3) 2.6 H2O in distilled water according to a certain molar ratio, adding a proper amount of NaOH solution into the distilled water, fully stirring to precipitate, and collecting; and uniformly mixing the collected precipitate, filtering, washing, drying, grinding and calcining to form the NiMn2O4 / NiO composite photocatalyst. According to the preparation method, the Z-type NiMn2O4 / NiO composite photocatalyst is prepared in situ through a simple one step, a tight interface is formed between NiMn2O4 and NiO through in-situ construction, and electron transfer of a system is facilitated. The method for preparing the composite photocatalyst is simple and convenient, the effective Z-type obtaining rate is high, the catalyst crystal form structure is good, and organic pollutants in water can be efficiently photocatalytically degraded under sunlight.

This invention relates to the field of geological exploration coring equipment technology, specifically to a coring device and method for shale gas and tight sandstone gas exploration. The coring device includes an outer tube, an inner tube, a reduced-diameter sleeve, a clamp-type core claw, a guide cylinder, a guide rod, a core break cutter, and a linkage assembly. The reduced-diameter sleeve is located at the bottom of the inner tube and inside the outer tube; the clamp-type core claw is installed on the reduced-diameter surface; the guide cylinder is inclined upwards and mounted on the reduced-diameter sleeve; the guide rod passes through the guide cylinder; the core break cutter is located at the bottom of the guide rod; when the clamp-type core claw moves downwards, it drives multiple sets of guide rods to move inwards synchronously through the linkage assembly. The multiple sets of guide rods and the core break cutter of this invention form a bottoming structure inwards, which supports the core inside the inner tube, enabling the relatively complete extraction of cores from interlayered shale reservoirs and tight sandstone reservoirs.