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Novel method for improving achievement rate of first backcross hybrid generation of oryza sativa and oryza minuta

A technology for cultivating rice and wild rice, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of difficulty in obtaining the first generation of backcross hybrids, increase the number of pollinated florets, be easy to grasp, and improve pollination methods Effect

Inactive Publication Date: 2012-09-19
广西壮族自治区农业科学院水稻研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a new method for improving the yield of hybrids of the first generation of backcrossing between cultivated rice and O. hard to get problem

Method used

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  • Novel method for improving achievement rate of first backcross hybrid generation of oryza sativa and oryza minuta
  • Novel method for improving achievement rate of first backcross hybrid generation of oryza sativa and oryza minuta
  • Novel method for improving achievement rate of first backcross hybrid generation of oryza sativa and oryza minuta

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The distant hybridization of the cultivated rice indica IR24 and O. sativa was carried out in Wuchang, Hubei. Due to the strong photosensitivity of the small-grained wild rice, the best heading period of the small-grained wild rice is from late September to early October under natural conditions, and short-light treatment is required for hybridization in Wuchang. Hybridization can be arranged in early September with the help of greenhouses and short light treatments.

[0034] When the flowering period of cultivated rice IR24 and wild rice agaricus meet, after 4:00 p.m., select 5-8 plants of IR24 with more panicles, relatively neat panicle layers, and flowering that day, and first use scissors to remove the plants that have opened that day. For the spikelets, treat the ears of rice with warm water at 44°C-46°C for 4-5 minutes to kill males, then manually cut glumes (cut off the upper 1 / 3 of each spikelet with scissors) and bagging ( Use a 28cm long and 10cm wide sodium ...

Embodiment 2

[0048] The distant hybridization of the cultivated rice japonica Nongken 58 and O. argentina was carried out in Wuchang, Hubei. Due to the strong photosensitivity of the small-grained wild rice, the best heading period of the small-grained wild rice is from late September to early October under natural conditions, and short-light treatment is required for hybridization in Wuchang. Hybridization can be arranged in early September with the help of greenhouses and short light treatments.

[0049] When the cultivated rice Nongken 58 meets the cultivated rice Nongken 58 at the flowering stage, the hybridization method is the same as that in Example 1.

[0050] After 10-12 days of hybrid immature embryo development, the enlarged ovary in the spikelet was taken out for embryo rescue. The method of embryo rescue is the same as in Example 1.

[0051] The first-generation hybrid of the cultivated rice Nongken 58 and the wild rice agaricus (BC 1 f 1 ) is obtained. The best heading p...

Embodiment 3

[0058] The distant hybridization between the cultivated rice indica Jin 23B and the wild rice O. sativa was carried out in Wuchang, Hubei. Due to the strong photosensitivity of the small-grained wild rice, the best heading period of the small-grained wild rice is from late September to early October under natural conditions, and short-light treatment is required for hybridization in Wuchang. Hybridization can be arranged in early September with the help of greenhouses and short light treatments.

[0059] When the cultivated rice Jin 23B and the cultivated rice Jin 23B meet at the flowering stage, the wild rice and the cultivated rice Jin 23B are hybridized, and the method of hybridization is the same as that in Example 1.

[0060] After 10-12 days of hybrid immature embryo development, the enlarged ovary in the spikelet was taken out for embryo rescue, and the embryo rescue method was the same as in Example 1.

[0061] The first-generation hybrid (BC) of rice Jin 23B and O. ...

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Abstract

The invention belongs to the technical field of cross breeding of plants and relates to a novel method for improving an achievement rate of a first backcross hybrid generation of oryza sativa and oryza minuta. The method includes that firstly, the oryza sativa is used as a female parent, the oryza minuta is used as a male parent, and the oryza sativa and the oryza minuta are subjected to hybridization and embryo rescue to obtain an interspecific hybrid F1 plant; secondly, the interspecific hybrid F1 plant is used as a female parent, the corresponding oryza sativa is used as a male parent, the interspecific hybrid F1 plant and the corresponding oryza sativa are subjected to backcross, by means of a method which is similar to a method of hybrid rice seed production, and the pollination is directly performed for 3 consecutive days to obtain a plurality of young embryos of a first wide cross and backcross hybrid generation; and finally, the plurality of young embryos are subjected to the embryo rescue to obtain the first backcross hybrid generation BC1F1 plant. According to the novel method for improving the achievement rate of the first backcross hybrid generation of the oryza sativa and the oryza minuta, the method is simple in operating and low in cost, the achievement rate can be stably improved from current 0.02% -0.03% to 0.32%-0.35%, and the problem that the first backcross hybrid generation BC1F1 of the oryza sativa and the oryza minuta is difficult to achieve is solved.

Description

technical field [0001] The invention belongs to the technical field of plant cross-breeding, and in particular relates to a new method capable of significantly improving the yield of first-generation hybrids obtained by backcrossing cultivated rice and small-grained wild rice. Background technique [0002] There are currently 22 wild species of Oryza, each with 10 different genomes (AA, BB, BBCC, CC, CCDD, EE, FF, GG, HHJJ, and HHKK), containing rich genetic diversity and many Excellent traits are valuable resources for genetic improvement and genome research of cultivated rice. Oryza sativa with karyotype BBCC is tetraploid and has the characteristics of resistance to rice blast, bacterial blight, sheath blight, brown planthopper, white-backed planthopper and leafhopper, and is an excellent germplasm resource. It has important utilization value in breeding. However, due to the great genetic differences between O. argentina and cultivated rice, there is a certain degree of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H4/00
Inventor 郭嗣斌高国庆韦宇李孝琼刘开强陈彩虹
Owner 广西壮族自治区农业科学院水稻研究所
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