Protein associated with rice seed shattering as well as coding genes and application thereof

A technique of encoding genes and shattering, applied in the field of protein and its encoding genes, can solve the problems of poor knowledge about the genetic structure and evolution history of African cultivated rice populations, and the lack of research on African cultivated rice

Inactive Publication Date: 2018-09-28
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, compared with Asian cultivated rice, the related research on African cultivated rice is very scarce, and little is known about the population genetic structure and evolutionary history of African cultivated rice

Method used

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  • Protein associated with rice seed shattering as well as coding genes and application thereof
  • Protein associated with rice seed shattering as well as coding genes and application thereof
  • Protein associated with rice seed shattering as well as coding genes and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Embodiment 1, the localization of regulation African rice grain shattering gene

[0058] African cultivated rice variety IRGC104165 and African wild rice W1411 ( figure 1 ) hybridization constructed a F containing 168 individuals 2 Separate groups. in this F 2 In the population, grain shattering was closely linked with molecular markers RM5626 and MR81, and finally the SH3 gene was located between these two markers. Then, using the 2650 recessive individuals in the expanded segregation population, and designing primers based on the sequence between the markers RM5626 and MR81, the SH3 gene was finally positioned between the two markers SNP29 and SNP31 ( figure 2 ). In Oryza sativa IRGC104165, the physical distance between these two markers is 17kb. According to the sequence prediction of Oryza sativa IRGC96717 (CG14) on the website http: / / www.softberry.com / , there is no candidate gene in this interval. In Oryza africa W1411, the physical distance between these tw...

Embodiment 2

[0059] Example 2, the acquisition of SH3 transgenic African rice and its functional verification

[0060] 1. Acquisition of SH3 gene sequence

[0061] Primers were designed according to the SH3 gene and promoter sequence of African wild rice W1411, and the primer sequences are as follows:

[0062] F: 5'-tgcagagacttccgggttga-3' (1-20 of SEQ ID No.2);

[0063] R: 5'-taagacggacgattaaagtt-3' (reverse complementary sequence of positions 11802-11821 of SEQ ID No. 2).

[0064] The whole genome DNA of African wild rice W1411 was extracted by CTAB method. Using this DNA as a template, a DNA fragment with a length of about 12 kb is amplified, and its sequence is SEQ ID No.2.

[0065] The 1-2208th positions of SEQ ID No.2 are the endogenous SH3 gene promoter sequences of African wild rice W1411, and the 2209-11122nd positions are the SH3 gene sequences containing introns (the 2284-6027th, 6151-7505th , 7633-10168, 10218-10378, 10455-11014 are intron sequences). The corresponding CDS...

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Abstract

The invention discloses a protein associated with rice seed shattering as well as coding genes and application thereof. The protein provided by the invention is any one as follows: a) protein comprising an amino acid sequence as shown in SEQ ID No.1; b) protein of which one or more amino acid residues of the amino acid sequence defined in a) is/are substituted and/or deleted and/or added, and which is associated with seed shattering of plant seeds; c) protein having 95% or more, 90% or more, 85% or more, or 80% or more homology with the amino acid sequence defined in any one of a) to b), and which is associated with the seed shattering of the plant seeds. An SH3 protein and the coding genes thereof, provided by the invention, are of great significance on regulating the seed shattering of the plant seeds, and play an important role in cultivating new varieties of high-yield plants.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering and relates to a protein related to rice shattering and the application of the coding gene thereof. Background technique [0002] The genus Oryza (Oryza L.) contains two cultivars, O. sativa L. and Oryza glaberrima Steud., which originated independently in Asia and Africa, respectively. Asian cultivated rice has a high yield and is widely planted all over the world, while African cultivated rice has a low yield and is limited to West Africa. [0003] Oryza africanus is a cultivated rice that originated independently in Africa about 3000 years ago, and oryza sativa was domesticated from a different ancestral species. In the middle of the 16th century, the Portuguese introduced Asian cultivated rice to West Africa, which led to a sharp decline in the species of African cultivated rice. Therefore, the planting area of ​​African cultivated rice is much smaller than that of Asian cultivated ri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00A01H6/46
CPCC07K14/415C12N15/8213C12N15/8261
Inventor 朱作峰吕树伟孙传清才宏伟谭禄宾
Owner CHINA AGRI UNIV
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