A molecular improvement method for reducing rice grain shattering
A technology of rice shattering and rice, which is applied in the field of rice biotechnology breeding, can solve the problems of harvest yield reduction, shattering harvest yield loss, easy grain drop, etc., to save time and cost, improve work efficiency, and genome DNA The effect of little damage
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[0029] The present invention will be further clarified through the detailed description of specific embodiments below, but it is not intended to limit the present invention, but only for illustration.
[0030] The present invention uses the CRISPR / Cas9 system to target and modify the rice shattering gene qSH1 to reduce the molecular genetic improvement method of rice (indica rice variety R1128) shattering, which includes the following steps:
[0031] 1) According to the Nipponbare qSH1 reference sequence, the double-stranded fragment of the indica rice R1128qSH1 coding region was cloned and sequenced;
[0032] 2) According to the above sequencing and splicing results, design two target sequences in the R1128qSH1 coding region and the upstream region of the 5' end of the start codon:
[0033] Target-qSH1-1 (SEQ ID NO.1): GCGCCATGTCGTCCGCCGCT
[0034] Target-qSH1-5 (SEQ ID NO.2): ACATGGCGCGCACGCACGTA
[0035] The above two target sequences both contain 5'-(N) X -NGG-3' st...
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