123 results about "Embryo rescue" patented technology

The invention relates to a method for high-efficiently cultivating excellent later generation of the wild rice distant hybridization , which is to cultivate the wide rice distant-hybridization high-efficient excellent later generation through the hybridization of the cultivated rice, the embryo rescue, the strong seedling culture, the pollen culture and the molecular marker assisted selection of the hybridization later generation of the cultivated rice and the wide rice. The survival rate of the distant hybridization of the invention is high; the hybrid progeny is backcrossed at the preliminary period and then self-bred, thus being beneficial to overcome the reproductive barrier caused by the different genome in the hybrid progeny of the wide rice and the cultivated rice, and to stabilize more genetic characters of the wide rice inside the cultivated rice; the pollen culture can accelerate the stability of the purification of the hybrid progeny; with the established molecular marker assisted selection, the breeding can be accurately performed without being influenced by the environmental conditions and being restricted by the growth and development period.

The invention discloses a culture method for triploid grape culture by embryo rescue and early identification of ploidy. The invention aims to cultivate triploid grape by embryo rescue and overcome the problem of seed abortion of hybrid embryo between diploid and tetraploid grape cultivars in conventional breeding. After a hybrid seed is obtained, chromosome count is performed by the combination of flow cytometry and wall degradation hypotonic method to identify the ploidy of the hybrid progeny. The invention increases the breeding efficiency of triploid grape, accelerates the breed selection of triploid grape variety and provides a new approach to the culture of seedless grape variety.

The invention discloses a sweet potato distant hybridization breeding method with a high success rate, and relates to the technical field of plant innovation and new variety breeding of crops. The method comprises the following steps of: taking a wide-compatibility parental sweet potato Xushu 18 as a female parent, and regulating the flower season of the Xushu 18 to be synchronous with that of a wild specie sweet potato by a grafting and dark treatment method; performing water culture on a male parent and the female parent indoor to naturally take root and blossom; after the male parent and the female parent blossom, performing hybrid pollination; after pollinating, coating a hybridization treating fluid (40mg/L 6-benzyladenine, 45mg/L 2,4-dichlorphenoxyacetic acid and 40mg/L naphthylacetic acid) on the stem base of an ovary and the whole flower stalk, and continuously treating for 7 days; and for a combined hybridized ovule which stops early development, adopting immature embryo rescue technology. The hybridization method and a hybridization system of the invention have the advantages of high operability and high success rate. The method is also suitable for indoor directed hybridization of a field naturally blossoming sweet potato material and a conventional sexual hybridization breeding of the sweet potato.

The present invention relates to a high-efficient breeding technique of Eurasian raisin grape. After the raisin grape has flowered for 30-50d, the young fruit of the grape is picked. The ovule is taken out in a sterilized state. The embryo is inoculated to an MS culture medium containing different levels of plant growth regulators of gibberellic acid (GA3), indoleacetic acid (IAA) and 6-benzylaminopurine (6-BA) for executing embryo rescue. The average embryo development rate using the breeding technique of the invention is increased from 8.3-11.9% of a patient disclosed in the background art to 84%. The germination rate obtains 75.33% and the seedling rate obtains 74.17%. Furthermore the embryo rescue technique of the raisin grape obtained through the technique of the invention has the advantages of increased seedling rate of offspring, easier obtainment of more filial generation, increased parent selection range of raisin grape cross breeding, accelerated breeding process of new species of the raisin grape and increased breeding efficiency of the raisin grape through increasing the embryo development rate and the germination rate of the raisin grape.

The invention discloses a method for increasing breeding efficiency of wild rice and cultivated rice distant hybridization embryo rescue, belongs to the field of breeding by the biological technique, and aims at reducing the alcohol disinfecting time and reducing the alcohol concentration as well as creating foundation for the survival rate of hybridization seedlings. The method is that the MS of an embryo culture is improved; the concentration of KNO3, NH4NO3, KH2PO4 and MnSO4.4H2O is reduced; meanwhile, CuSO4.5H2O, CoCl2.6H2O, niacin, pyridoxine hydrochloride and thiamine hydrochloride are not added; the hardening-seedling technology is innovated; when in hardening seedling of the plants, the hardening-seedling treatment with convenient operation and good effect is carried out, so that the survival rate of the seedlings can be greatly increased. According to the operation of the method, the operation of embryo rescue is simplified; the whole culture costs 30 days only, so that the culture cycle can be greatly reduced; meanwhile, the seedling rate can be greatly increased and up to 94%; therefore, the breeding efficiency of the wild rice and cultivated rice distant hybridization embryo rescue can be increased.

The invention belongs to the technical field of cross breeding of plants and relates to a novel method for improving an achievement rate of a first backcross hybrid generation of oryza sativa and oryza minuta. The method includes that firstly, the oryza sativa is used as a female parent, the oryza minuta is used as a male parent, and the oryza sativa and the oryza minuta are subjected to hybridization and embryo rescue to obtain an interspecific hybrid F1 plant; secondly, the interspecific hybrid F1 plant is used as a female parent, the corresponding oryza sativa is used as a male parent, the interspecific hybrid F1 plant and the corresponding oryza sativa are subjected to backcross, by means of a method which is similar to a method of hybrid rice seed production, and the pollination is directly performed for 3 consecutive days to obtain a plurality of young embryos of a first wide cross and backcross hybrid generation; and finally, the plurality of young embryos are subjected to the embryo rescue to obtain the first backcross hybrid generation BC1F1 plant. According to the novel method for improving the achievement rate of the first backcross hybrid generation of the oryza sativa and the oryza minuta, the method is simple in operating and low in cost, the achievement rate can be stably improved from current 0.02% -0.03% to 0.32%-0.35%, and the problem that the first backcross hybrid generation BC1F1 of the oryza sativa and the oryza minuta is difficult to achieve is solved.

A method for preparing the distant hybrid of chrysanthemum by embryo rescue includes hybridization between wild diplontic chrysanthemum and cultured hexaplontic 'Huang Ying', embryo rescue by ovary culture, discriminating their descendants by different approaches, and choosing the descendant with new characters.

The invention provides a method of construction of allopolyploid rice through combination of embryo rescue and in-vitro induction. The method includes the following steps: a, disposing wild rice under dark conditions to enable the flowering stage of the wild rice and the flowering stage of cultivated rice to converge; b, hybridizing the cultivated rice with the wild rice; c, after the hybridization, spraying young embryo development promoting fluid; d, carrying out embryo rescue cultivation; e, hybrid embryos send forth sprouts or calluses; f, treating the sprouts or the calluses with colchicines; i, transplanting tissue culture seedlings; j, selecting heterogenous hybrid seedlings; and k, determining allotetraploid rice or allohexaploid rice. Through the method, a plurality of species of heterogeneous hybrids and allopolyploids among a plurality of genomes can be obtained, and the method develops a new path for crossbreeding of cultivated rice/wild rice and full utilization of favorable genes of the wild rice.

The invention relates to a method for achieving isolated embryos through radish and turnip intergeneric distant hybridization. The method is characterized by sequentially comprising the following steps of: (1) preparing materials; (2) sowing; (3) bagging and hanging plates; (4) performing pollination; and (5) saving embryos. By the method, the radish and turnip intergeneric distant hybridization isolated embryos are obtained successfully; hybrid embryo seedlings are cultivated; and a new breeding resource is obtained.

The invention discloses a novel germplasm creating method by distant hybridization of Brassica campestris ssp. chinensis Makino and Raphanus sativus. The method uses autotetraploid Brassica campestris ssp. chinensis Makino as a female parent and autotetraploid Raphanus sativus as a male parent to conduct artificial sexual crossbreeding; and repeated pollination and in vitro embryo rescue techniques are employed to obtain distant hybridization novel germplasm of autotetraploid Brassica campestris ssp. chinensis Makino and autotetraploid Raphanus sativus. The invention for the first time adopts sexual crossbreeding of the autotetraploid Brassica campestris ssp. Chinensis Makino and autotetraploid Raphanus sativus and in vitro embryo rescue technique to obtain new germplasm of distant hybridization. Compared with traditional diploid hybridization, which obtains zygotic embryo for ploidy reduplication, the method does not require chromosome doubling operation, and can significantly shorten the breeding time; and the hybrid F1 can reach stable inheritance. The novel germplasm created by the invention creation has the genetic materials of Brassica campestris ssp. chinensis Makino and Raphanus sativus, and a wider genetic diversity.

The invention discloses a method for obtaining a poinsettia filial generation through utilizing young embryo rescue. The method is characterized in that 15-22d of ovules which are subjected to artificial cross pollination are subjected to young embryo rescue through adopting in-vitro culture; and the method comprises the following specific steps of: (1) ovule stripping, (2) induction culturing, (3) subculturing, multiplying and rooting, and (4) transplanting. By utilizing the method, poinsettia hybrid ovules are effectively prevented from premature failure, the hybrid survival rate of the poinsettia filial generation is improved, the culture period of the poinsettia filial generation is shortened, and the method can be applied to poinsettia hybridization breeding.

The invention relates to a method for performing isolated culture on radish and kohlrabi intergeneric hybrid embryos. The method is characterized by sequentially comprising the following steps of: (1) preparing materials; (2) sowing; (3) hybridizing; and (4) saving embryos. By the method, the radish and kohlrabi intergeneric hybrid embryos are obtained successfully; hybrid embryo seedlings are cultivated; and a new breeding resource is obtained.

The invention relates to a method for getting an excised embryo by virtue of intergeneric distant hybridization between radish and kohlrabi, and the method is characterized by comprising the following steps in sequence: (1) preparing materials; (2) seeding; (3) bagging and hanging out a plate; (4) pollinating; and (5) performing embryo rescue. By utilizing the method provided by the invention, the excised embryo formed by virtue of intergeneric distant hybridization between the radish and the kohlrabi can be successfully obtained, and a hybrid embryo seedling is further cultured, thus the method provided by the invention is conductive to getting breeding new resources.

The invention relates to a method for improving the domestication survival rate of distant-hybridization tissue cultured seedlings of cucumbers, belonging to the field of biotechnology. The method is mainly used for propagation and preservation and domestication seedling of hybridization materials of cucumber species and provides technical guarantee for application of a distant hybridization technology to cucumber breeding. The method mainly includes the steps of performing embryo rescue to obtain aseptic seedlings, carrying out induced differentiation on adventitious buds, carrying out regeneration bud extension and rooting culture and domestication transplanting, and regulating and controlling a specially prepared culture medium and external environment conditions. The method is simple in operation, has low requirements on raw materials and laboratory facilities, and has extremely good effects. According to the method, the obtained distant-hybridization tissue cultured seedlings have strong functional roots, the domestication period is shortened, and the survival rate of transplanting is improved. The method can provide a stable and high-efficiency regeneration seedling domestication system for hybridization of two individuals from different cucumber species and hybridization of two individuals from the same cucumis genus, and can be used for preservation and propagation and preservation of treasured cucumis wild species and transplanting seedling of common cucumber tissue cultured seedlings.

The invention discloses a method for artificially synthesizing a novel anti-clubroot brassica napus material. The method includes: taking anti-clubroot brassica oleracea as a female parent to hybridize with anti-clubroot pakchoi; after 15 days of pollination of a generation F1, selecting seeds for embryo rescue, using a B5 fluid medium containing 2% of sucrose to culture immature seeds, and after immature embryos emerge and send up cotyledons, transferring the embryos to the B5 fluid medium containing 2% of sucrose, using 0.1% colchicines in 3-4-leaf stage to treat for 2h to realize doubling germination, cleaning, transferring into soil, and enabling selfing of synthesized plants by means of artificial pollination or saltwater spraying in a flowering stage; in a germination stage of selfing seeds, adopting a clubroot P4 physiological race for inoculation while taking an infected material as a contrast, inoculating at the same time, taking out seedling roots after 35 days, cleaning, observing whether lumps exist or not, and determining that the synthesized plants are resistant to clubroot if no lump exists. An effective approach is provided for breeding of anti-clubroot brassica napus strains, and clubroot inoculation efficiency is improved.

The invention relates to a method for cultivating Lilium oriental hybrid embryos, which is effective in breeding effect and short in breeding period. The method includes: pollinating a Lilium oriental hybrid line; sectioning and peeling small embryos, ovules or ovaries; and performing isolated culture. By cross pollination of the ovaries and young embryos and isolated culture, the embryos can be taken out for culturing before abortion to avoid premature senility of distant hybrid embryos, and accordingly Lilium post-fertilization barrier can be overcome effectively, a great amount of distant hybrid embryos are allowed to continue growing into normal seeds, a breeding procedure is drawn close as soon as possible, and the breeding cycle is shortened. In addition, a great amount of distant hybrids are obtained, Lilium interspecific distant hybridization is achieved, and a batch of novel germplasm is created. The embryo rescue technology and the technology of within-bottle Lilium bulbing are combined, so that culture cycle of Lilium hybrids is shortened effectively, and hybrid survival rate is increased.

The invention relates to a novel method for obtaining artificially synthesized hexaploid wheat. The novel method comprises the steps of distant hybridization and obtaining the artificially synthesized hexaploid wheat. The distant hybridization includes that the distant hybridization is carried out with aegilops tauschii as a female parent and common wheat as a male parent, young embryo rescue is carried out through a hybrid embryo, and a test-tube plantlet is obtained; and the test-tube plantlet grows over a summer, manual doubling germination is carried out in November by means of colchicines, normal cultivation management proceeds, and aegilops tauschii-wheat octaploid seeds are obtained the next year. The steps of obtaining the artificially synthesized hexaploid wheat include that hybrid is carried out by means of the obtained aegilops tauschii-wheat octaploid seeds and tetraploid wheat with various good genes, and the hexaploid wheat is directly manually obtained. The novel method is easy to carry out, and the hexaploid wheat germplasm with a rich genetic background can be conveniently and manually synthesized.

The invention discloses a method for obtaining a plant graft chimaera progeny by embryo rescue, which comprises the following steps of: (1) picking an ovary in 1 day before the immature embryo of a crucifer graft chimaera is aborted; (2) sterilizing and flushing the ovary; (3) placing the ovary on a culture dish paved with filter paper which is fully soaked in nutrient solution, dissecting one end of the ovary and then tearing the ovary along a ventral suture, selecting and cutting light green and glossy ovule, suspensor and ovary wall tissue connected with the suspensor and inoculating the three to a development culture medium, culturing the three under a dark condition at the temperature of 25 +/- 2 DEG C, and then culturing the three under light; (4) picking mature seeds, inoculating the seeds to a sprouting culture medium, and culturing the seeds under the dark condition at the temperature of 25 +/- 2 DEG C; (5) after the seeds are sprouted and grow main leaves, transferring the seedlings to a sub-cultured medium to perform culture, and then transferring the seedlings to a rooting culture medium to perform rooting; and (6) taking the routed seedlings out, transplanting the seedlings to a nutrition pot filled with matrix, hardening the seedlings for 6 to 12 days, and then transferring the seedlings to a field so as to obtain the plant graft chimaera progeny.

The invention discloses a method for realizing distant hybridization of adzuki beans and rice beans by utilizing young embryo rescue. The method comprises the steps of (1) preparation of materials, namely, selecting more early-matured rice bean material as female parent, selecting adzuki bean variety with excellent comprehensive characters as male parent, and selecting disease-free full grains for planting; (2) material planting; (3) cross pollination; (4) young embryo rescue; (5) hybrid seedling transplanting. According to the method, the more early-matured rice bean material is taken as the female parent, and the adzuki bean variety with excellent comprehensive characters is taken as the male parent, so that the obtained hybrid young embryos can directly grow into seedlings, the seedlings are good in growth and development and high in survival rate of transplanting, distant hybridization F1 plants can normally bloom and bear fruit at the adult-plant stage, and seeds of F2 can be harvested. After the method is used, a great deal of distant hybridization embryos can continue to develop into normal seeds, abortion of distant hybrid embryos can be effectively avoided, a great deal of distant hybridization filial generations can be obtained, and interspecific distant hybridization of the adzuki beans and the rice beans can be finally realized.

The invention provides a method of constructing clone groups of lily hybridization generation. The method comprises the following steps of cutting stigmas of screened lily target parents, hybridizing and collecting; performing embryo rescue such as germinating, multiplying and heading; expanding propagation from one plant to hundreds of plants; vernalizing after bulblets grow; cultivating the bulblets to soil of a greenhouse after treatment; overwintering twice; digging the bulblets out before the third winter; and thus obtaining hundreds of the individual flowering clone groups. Due to the adoption of the method, the period is greatly shortened from eleven years to five years, so that the breeding speed of lily is increased, and the efficiency of breeding new varieties of lily is improved. The method is simple in process, has obvious effects of obtaining lily distant hybridization generation of the lily and quickly constructing hybrid clone groups, and the traits of all plants in the groups are good consistently.

The invention discloses a biotechnological breeding method for obtaining antiviral seedless grapes. The biotechnological breeding method comprises the following steps: carrying out hybridization between different varieties of seedless grapes and embryo rescue to obtain grape zygotic embryos containing seedless genes; inducing grape somatic embryogenesis through the grape zygotic embryos; constructing RNAi antiviral plant expression vectors, and converting the RNAi antiviral plant expression vectors into agrobacterium tumefaciens; converting the grape somatic embryos sourced from the grape zygotic embryos by utilizing the agrobacterium tumefaciens, thereby obtaining regeneration plants. The biotechnological breeding method has the advantages that seedless grape embryo rescue technology and the transgenic technology are combined, embryoids which are sourced from seedless grape hybridization immature embryos and obtained through embryo rescue are taken as receptor materials, and the RNAi antiviral vectors containing the conserved gene segments of grape virus coat protein (CP) are utilized for genetic transformation of agrobacterium tumefaciens mediation, so that novel grape materials which are both antiviral and seedless can be obtained from the transformed regeneration plants, and then selectively breeding of the novel varieties can be realized.

The invention provides a medicinal wild rice embryo rescue rapid progeny propagation culture medium. The medicinal wild rice embryo rescue rapid progeny propagation culture medium comprises an induction culture medium, a differentiation culture medium and a rooting culture medium, wherein the formula of the induction culture medium is MS+2.4D1.0-3.0mg/l+6BA0.1-0.5mg/l; the formula of the differentiation culture medium is MS+IAA1.0-3.0mg/l+6BA2.0-4.0mg/l+KT1.0-2.0mg/l; and the formula of the rooting culture medium is 1/2MS+IAA0.02-0.5mg/l. The invention also provides a medicinal wild rice embryo rescue rapid progeny propagation method. According to the medicinal wild rice embryo rescue rapid progeny propagation culture medium and method provided by the invention, healthy and rapid growth of tissue culture seedlings can be realized a short period of time.