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123 results about "Embryo rescue" patented technology
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Embryo rescue is one of the earliest and successful forms of in-vitro culture techniques that is used to assist in the development of plant embryos that might not survive to become viable plants. Embryo rescue plays an important role in modern plant breeding, allowing the development of many interspecific and intergeneric food and ornamental plant crop hybrids. This technique nurtures the immature or weak embryo, thus allowing it the chance to survive. Plant embryos are multicellular structures that have the potential to develop into a new plant. The most widely used embryo rescue procedure is referred to as embryo culture, and involves excising plant embryos and placing them onto media culture. Embryo rescue is most often used to create interspecific and intergeneric crosses that would normally produce seeds which are aborted. Interspecific incompatibility in plants can occur for many reasons, but most often embryo abortion occurs In plant breeding, wide hybridization crosses can result in small shrunken seeds which indicate that fertilization has occurred, however the seed fails to develop. Many times, remote hybridizations will fail to undergo normal sexual reproduction, thus embryo rescue can assist in circumventing this problem.
Wild-rice distant hybridization high-efficient cultivating superior progeny method
InactiveCN101142894AStable homozygousAccelerated homozygosityMicrobiological testing/measurementHorticulture methodsAgricultural sciencePollen
The invention relates to a method for high-efficiently cultivating excellent later generation of the wild rice distant hybridization , which is to cultivate the wide rice distant-hybridization high-efficient excellent later generation through the hybridization of the cultivated rice, the embryo rescue, the strong seedling culture, the pollen culture and the molecular marker assisted selection of the hybridization later generation of the cultivated rice and the wide rice. The survival rate of the distant hybridization of the invention is high; the hybrid progeny is backcrossed at the preliminary period and then self-bred, thus being beneficial to overcome the reproductive barrier caused by the different genome in the hybrid progeny of the wide rice and the cultivated rice, and to stabilize more genetic characters of the wide rice inside the cultivated rice; the pollen culture can accelerate the stability of the purification of the hybrid progeny; with the established molecular marker assisted selection, the breeding can be accurately performed without being influenced by the environmental conditions and being restricted by the growth and development period.
Owner:云南省农业科学院生物技术与种质资源研究所
Breeding technique of obtaining triploid grape and ploidy early identification using embryo
InactiveCN101283669ASpeed up the breeding processImprove breeding efficiencyDead plant preservationMicrobiological testing/measurementVitis viniferaAgricultural science
The invention discloses a culture method for triploid grape culture by embryo rescue and early identification of ploidy. The invention aims to cultivate triploid grape by embryo rescue and overcome the problem of seed abortion of hybrid embryo between diploid and tetraploid grape cultivars in conventional breeding. After a hybrid seed is obtained, chromosome count is performed by the combination of flow cytometry and wall degradation hypotonic method to identify the ploidy of the hybrid progeny. The invention increases the breeding efficiency of triploid grape, accelerates the breed selection of triploid grape variety and provides a new approach to the culture of seedless grape variety.
Owner:NORTHWEST A & F UNIV
Sweet potato distant hybridization breeding method with high success rate
InactiveCN101715724AEasy to operateIncrease success rateBiocidePlant growth regulatorsFlowering seasonWild species
The invention discloses a sweet potato distant hybridization breeding method with a high success rate, and relates to the technical field of plant innovation and new variety breeding of crops. The method comprises the following steps of: taking a wide-compatibility parental sweet potato Xushu 18 as a female parent, and regulating the flower season of the Xushu 18 to be synchronous with that of a wild specie sweet potato by a grafting and dark treatment method; performing water culture on a male parent and the female parent indoor to naturally take root and blossom; after the male parent and the female parent blossom, performing hybrid pollination; after pollinating, coating a hybridization treating fluid (40mg / L 6-benzyladenine, 45mg / L 2,4-dichlorphenoxyacetic acid and 40mg / L naphthylacetic acid) on the stem base of an ovary and the whole flower stalk, and continuously treating for 7 days; and for a combined hybridized ovule which stops early development, adopting immature embryo rescue technology. The hybridization method and a hybridization system of the invention have the advantages of high operability and high success rate. The method is also suitable for indoor directed hybridization of a field naturally blossoming sweet potato material and a conventional sexual hybridization breeding of the sweet potato.
Owner:XUZHOU ACAD OF AGRI SCI
High-efficient breeding technique of Eurasian raisin grape
ActiveCN101564011AAvoid difficultiesEfficient Breeding TechnologyPlant tissue cultureHorticulture methodsGrowth plantIndoleacetic Acids
The present invention relates to a high-efficient breeding technique of Eurasian raisin grape. After the raisin grape has flowered for 30-50d, the young fruit of the grape is picked. The ovule is taken out in a sterilized state. The embryo is inoculated to an MS culture medium containing different levels of plant growth regulators of gibberellic acid (GA3), indoleacetic acid (IAA) and 6-benzylaminopurine (6-BA) for executing embryo rescue. The average embryo development rate using the breeding technique of the invention is increased from 8.3-11.9% of a patient disclosed in the background art to 84%. The germination rate obtains 75.33% and the seedling rate obtains 74.17%. Furthermore the embryo rescue technique of the raisin grape obtained through the technique of the invention has the advantages of increased seedling rate of offspring, easier obtainment of more filial generation, increased parent selection range of raisin grape cross breeding, accelerated breeding process of new species of the raisin grape and increased breeding efficiency of the raisin grape through increasing the embryo development rate and the germination rate of the raisin grape.
Owner:HANGZHOU BLUE SKY LANDSCAPE CONSTR GROUP
Method for increasing breeding efficiency of wild rice and cultivated rice distant hybridization embryo rescue
InactiveCN104871964ANot hurtImprove survival ratePlant tissue cultureHorticulture methodsAgricultural scienceThiamine hcl
The invention discloses a method for increasing breeding efficiency of wild rice and cultivated rice distant hybridization embryo rescue, belongs to the field of breeding by the biological technique, and aims at reducing the alcohol disinfecting time and reducing the alcohol concentration as well as creating foundation for the survival rate of hybridization seedlings. The method is that the MS of an embryo culture is improved; the concentration of KNO3, NH4NO3, KH2PO4 and MnSO4.4H2O is reduced; meanwhile, CuSO4.5H2O, CoCl2.6H2O, niacin, pyridoxine hydrochloride and thiamine hydrochloride are not added; the hardening-seedling technology is innovated; when in hardening seedling of the plants, the hardening-seedling treatment with convenient operation and good effect is carried out, so that the survival rate of the seedlings can be greatly increased. According to the operation of the method, the operation of embryo rescue is simplified; the whole culture costs 30 days only, so that the culture cycle can be greatly reduced; meanwhile, the seedling rate can be greatly increased and up to 94%; therefore, the breeding efficiency of the wild rice and cultivated rice distant hybridization embryo rescue can be increased.
Owner:云南省农业科学院生物技术与种质资源研究所
Novel method for improving achievement rate of first backcross hybrid generation of oryza sativa and oryza minuta
InactiveCN102668973AEasy to shatterReduced shatteringHorticulture methodsPlant tissue cultureOryzaAgricultural science
The invention belongs to the technical field of cross breeding of plants and relates to a novel method for improving an achievement rate of a first backcross hybrid generation of oryza sativa and oryza minuta. The method includes that firstly, the oryza sativa is used as a female parent, the oryza minuta is used as a male parent, and the oryza sativa and the oryza minuta are subjected to hybridization and embryo rescue to obtain an interspecific hybrid F1 plant; secondly, the interspecific hybrid F1 plant is used as a female parent, the corresponding oryza sativa is used as a male parent, the interspecific hybrid F1 plant and the corresponding oryza sativa are subjected to backcross, by means of a method which is similar to a method of hybrid rice seed production, and the pollination is directly performed for 3 consecutive days to obtain a plurality of young embryos of a first wide cross and backcross hybrid generation; and finally, the plurality of young embryos are subjected to the embryo rescue to obtain the first backcross hybrid generation BC1F1 plant. According to the novel method for improving the achievement rate of the first backcross hybrid generation of the oryza sativa and the oryza minuta, the method is simple in operating and low in cost, the achievement rate can be stably improved from current 0.02% -0.03% to 0.32%-0.35%, and the problem that the first backcross hybrid generation BC1F1 of the oryza sativa and the oryza minuta is difficult to achieve is solved.
Owner:广西壮族自治区农业科学院水稻研究所
Method for obtaining chrysanthemum distance hydrid using goung embryo rescue
InactiveCN1561694ABroaden the genetic baseDistant hybridizationPlant genotype modificationGermplasmInterspecific hybridization
A method for preparing the distant hybrid of chrysanthemum by embryo rescue includes hybridization between wild diplontic chrysanthemum and cultured hexaplontic 'Huang Ying', embryo rescue by ovary culture, discriminating their descendants by different approaches, and choosing the descendant with new characters.
Owner:NANJING AGRICULTURAL UNIVERSITY
Interspecific hybrid embryo rescuing and seedling forming method of sweet cherries in southern China and Chinese cherries
PendingCN110050690ALearn about developmental statusDetermine the best time for developmentCultivating equipmentsPlant tissue cultureAnthriscus cerefoliumShoot
The invention relates to the field of cross breeding of plants and particularly relates to an interspecific hybrid embryo rescuing and seedling forming method of sweet cherries in southern China and Chinese cherries. The method combines advantages of European sweet cherries and the Chinese cherries, and takes the 'European sweet cherries' as a female parent and the 'Chinese cherries' as a male parent to carry out interspecific distant hybridization. Aiming at the abortion problem of a distant hybrid embryo, the hybrid embryo is subjected to embryo rescuing. A proper sampling period of the embryo rescuing of each hybrid combination, concentrations and ratios of respective exogenous growth regulators for hybrid young embryo germination, subculture propagation and rooting culture, and a proper acclimatization and transplanting method are determined. By applying an interspecific hybrid embryo rescuing system of the sweet cherries in southern China and the Chinese cherries, which is established by the technology, the embryo germination rate reaches 80.00 percent, the multiplication coefficient of subculture hybridized new shoots reaches 5.0, the rooting rate of rooting culture is 90.91percent, the growth vigor of embryo culture seedlings is good and the seedlings grow robustly and have green leaves, and the acclimatization and transplanting survival rate reaches 77.27 percent.
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Method of construction of allopolyploid rice through combination of embryo rescue and in-vitro induction
The invention provides a method of construction of allopolyploid rice through combination of embryo rescue and in-vitro induction. The method includes the following steps: a, disposing wild rice under dark conditions to enable the flowering stage of the wild rice and the flowering stage of cultivated rice to converge; b, hybridizing the cultivated rice with the wild rice; c, after the hybridization, spraying young embryo development promoting fluid; d, carrying out embryo rescue cultivation; e, hybrid embryos send forth sprouts or calluses; f, treating the sprouts or the calluses with colchicines; i, transplanting tissue culture seedlings; j, selecting heterogenous hybrid seedlings; and k, determining allotetraploid rice or allohexaploid rice. Through the method, a plurality of species of heterogeneous hybrids and allopolyploids among a plurality of genomes can be obtained, and the method develops a new path for crossbreeding of cultivated rice / wild rice and full utilization of favorable genes of the wild rice.
Owner:HUBEI UNIV +1
Method for achieving isolated embryos through radish and turnip intergeneric distant hybridization
InactiveCN102210259AHigh affinityPromote innovationPlant tissue cultureHorticulture methodsPollinationSeedling
The invention relates to a method for achieving isolated embryos through radish and turnip intergeneric distant hybridization. The method is characterized by sequentially comprising the following steps of: (1) preparing materials; (2) sowing; (3) bagging and hanging plates; (4) performing pollination; and (5) saving embryos. By the method, the radish and turnip intergeneric distant hybridization isolated embryos are obtained successfully; hybrid embryo seedlings are cultivated; and a new breeding resource is obtained.
Owner:INST OF ECONOMIC CROP HUBEI ACADEMY OF AGRI SCI
Method for fast cultivating abies beshanzuensis seedlings through embryo rescue technology
ActiveCN108552056AOvercoming the defects of abortionEasy to operatePlant tissue cultureHorticulture methodsAbies beshanzuensisOperability
The invention discloses a method for fast cultivating abies beshanzuensis seedlings through the embryo rescue technology, and aims at solving the problem that abies beshanzuensis is critically endangered. According to the method, immature cones of wild plants of the abies beshanzuensis in the Baishanzu nature reserve are adopted as starting materials, immature embryos and endosperm of the immaturecones are picked off after surface disinfection is carried out, wild plant offsprings of the abies beshanzuensis are fast cultivated through the embryo rescue technology, and a large number of abiesbeshanzuensis seedlings are obtained within a short time. The method has the advantages that the immature embryos of the abies beshanzuensis are utilized, after cultivation is carried out for 30 days,many abies beshanzuensis seedlings are obtained, the cultivation speed of the abies beshanzuensis seedlings is increased, and the current situation that the abies beshanzuensis is critically endangered is relieved. The method is high in operability, simple, efficient and low in pollution rate, and has important significance on fast cultivation of threatened plant seedlings in the world.
Owner:ZHEJIANG UNIV
Novel germplasm creating method by distant hybridization of Brassica campestris ssp. chinensis Makino and Raphanus sativus
InactiveCN103947536AGenetic stabilityShorten breeding timePlant tissue cultureHorticulture methodsBrassicaGenetic diversity
The invention discloses a novel germplasm creating method by distant hybridization of Brassica campestris ssp. chinensis Makino and Raphanus sativus. The method uses autotetraploid Brassica campestris ssp. chinensis Makino as a female parent and autotetraploid Raphanus sativus as a male parent to conduct artificial sexual crossbreeding; and repeated pollination and in vitro embryo rescue techniques are employed to obtain distant hybridization novel germplasm of autotetraploid Brassica campestris ssp. chinensis Makino and autotetraploid Raphanus sativus. The invention for the first time adopts sexual crossbreeding of the autotetraploid Brassica campestris ssp. Chinensis Makino and autotetraploid Raphanus sativus and in vitro embryo rescue technique to obtain new germplasm of distant hybridization. Compared with traditional diploid hybridization, which obtains zygotic embryo for ploidy reduplication, the method does not require chromosome doubling operation, and can significantly shorten the breeding time; and the hybrid F1 can reach stable inheritance. The novel germplasm created by the invention creation has the genetic materials of Brassica campestris ssp. chinensis Makino and Raphanus sativus, and a wider genetic diversity.
Owner:NANJING AGRICULTURAL UNIVERSITY
Method for obtaining poinsettia filial generation through utilizing young embryo rescue
InactiveCN102845312AOvercoming difficultiesOvercome difficult phenomenaCultivating equipmentsPlant tissue culturePoinsettiaPollination
The invention discloses a method for obtaining a poinsettia filial generation through utilizing young embryo rescue. The method is characterized in that 15-22d of ovules which are subjected to artificial cross pollination are subjected to young embryo rescue through adopting in-vitro culture; and the method comprises the following specific steps of: (1) ovule stripping, (2) induction culturing, (3) subculturing, multiplying and rooting, and (4) transplanting. By utilizing the method, poinsettia hybrid ovules are effectively prevented from premature failure, the hybrid survival rate of the poinsettia filial generation is improved, the culture period of the poinsettia filial generation is shortened, and the method can be applied to poinsettia hybridization breeding.
Owner:JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
Method for performing isolated culture on radish and kohlrabi intergeneric hybrid embryos
InactiveCN102210258APromote innovationPlant tissue cultureHorticulture methodsSeedlingRadish (Vegetable)
The invention relates to a method for performing isolated culture on radish and kohlrabi intergeneric hybrid embryos. The method is characterized by sequentially comprising the following steps of: (1) preparing materials; (2) sowing; (3) hybridizing; and (4) saving embryos. By the method, the radish and kohlrabi intergeneric hybrid embryos are obtained successfully; hybrid embryo seedlings are cultivated; and a new breeding resource is obtained.
Owner:INST OF ECONOMIC CROP HUBEI ACADEMY OF AGRI SCI
Method for creating yellow-seeded brassica napus germplasm
InactiveCN102301946AHas the source of yellow seed geneHigh frequencyMicrobiological testing/measurementPlant genotype modificationBiotechnologyBrassicaceae
Owner:JIANGSU ACAD OF AGRI SCI
Method for getting excised embryo by virtue of intergeneric distant hybridization between radish and kohlrabi
InactiveCN102210260AHigh affinityPromote innovationPlant tissue cultureHorticulture methodsBiologySeedling
The invention relates to a method for getting an excised embryo by virtue of intergeneric distant hybridization between radish and kohlrabi, and the method is characterized by comprising the following steps in sequence: (1) preparing materials; (2) seeding; (3) bagging and hanging out a plate; (4) pollinating; and (5) performing embryo rescue. By utilizing the method provided by the invention, the excised embryo formed by virtue of intergeneric distant hybridization between the radish and the kohlrabi can be successfully obtained, and a hybrid embryo seedling is further cultured, thus the method provided by the invention is conductive to getting breeding new resources.
Owner:INST OF ECONOMIC CROP HUBEI ACADEMY OF AGRI SCI
Method for embryo rescue at different developmental stages of early phase of lilium formolongi distant hybridization
ActiveCN105123528AOvercome obstaclesVariety of colorsHorticulture methodsPlant tissue cultureDevelopmental stageLilium formolongi
The invention belongs to the field of tissue culture of plants and particularly provides a method for embryo rescue at different developmental stages of the early phase of lilium formolongi distant hybridization. The method comprises steps as follows: 1), after lilium formolongi distant hybridization, ovaries are taken to be sterilized and disinfected; 2), embryo rescue at different developmental stages is performed in 5-25 days after pollination as follows: the ovaries are sliced and cultured, ovules are obtained and subjected to tissue culture, and hybridized tissue culture seedlings are obtained; embryo rescue at different developmental stages is performed in 35-40 days after pollination as follows: in vitro culture of ovules or in vitro culture of embryos is performed, and the hybridized tissue culture seedlings are obtained. The invention further provides an application of the method for embryo rescue at different developmental stages of the early phase of lilium formolongi distant hybridization in lily breeding. According to the method for embryo rescue at different developmental stages of the early phase of lilium formolongi distant hybridization, the barrier of incompatibility after distant hybridization fertilization of lilium formolongi and lilium oriental hybrid lines can be overcome, and the ovules with the embryos and hybrid seedlings are obtained.
Owner:BEIJING FORESTRY UNIVERSITY
Method for obtaining distant hybridization offspring of common head cabbage and cabbage type rape
InactiveCN108308020ARestore fertilityShorten the rescue induction timeHorticulture methodsPlant tissue cultureGermplasmBud
The invention provides a method for obtaining a distant hybridization offspring of a common head cabbage and a cabbage type rape, and belongs to the field of biotechnological breeding. The method comprises the steps that (1) a common head cabbage cytoplasmic male sterility is selected to serve as a female parent, a cabbage type rape restorer serves as a male parent, and the female parent and the male parent are isolated after hand pollination is conducted; (2) an ovary after being pollinated for 18-25 days is cut and disinfected; (3) in-vitro culture is conducted for 18-25 days; (4) an ovule in the ovary which is subjected to in-vitro culture is peeled off, and then subjected to induction culture to germinate; (5) differentiation culture is conducted, and a F1 generation seedling is obtained; and (6) a lateral bud of the F1 generation seedling is separated and transplanted onto a rooting medium, and a complete hybridization seedling is obtained. The method utilizes an embryo rescue technique to overcome the problem that the hybridization seedling cannot be obtained due to abortion of a young embryo is prone to occurring in the distant hybridization process of the common head cabbage and the cabbage type rape, an excellent recovery gene of the rape is transferred into a common head cabbage cytoplasmic male sterile cultivated species, the fertility of cabbage is recovered, and the technical evidences are provided for innovation and application of germplasm resources of cabbage.
Owner:NORTHWEST A & F UNIV
Pickle and purple cabbage trigenomic species allohexaploid vegetable germplasm and acquisition method
ActiveCN103651111AShorten the timeEasy to operateHorticulture methodsPlant tissue cultureGermplasmOperability
The invention discloses a pickle and purple cabbage tri-genome species allohexaploid vegetable germplasm and an acquisition method, which fill in the blank of lack of brassica trigenomic species allohexaploid vegetable germplasm. The acquisition method comprises the steps that pickle and purple cabbages of brassica vegetable germplasm are cross-fertilized; an ovary is picked at the seventh day after pollination; mature seeds are obtained by embryo rescue; a large quantity of propagated progenies are obtained through subculture and propagation after seed germination; duplication treatment is carried out on a haploid by a liquid culture medium with colchicine to obtain the multi-genome allohexaploid vegetable germplasm. The pickle and purple cabbage tri-genome species allohexaploid vegetable germplasm and the acquisition method, disclosed by the invention, have the advantages of strong operability, simpleness and practicality, save the time for obtaining multi-genome polyploidy material, can be widely used for embryo rescue for interspecies cross of cruciferous brassica vegetable germplasm, and have great significance to cultivation and breeding of new species of special plants.
Owner:ZHEJIANG UNIV
Method for improving domestication survival rate of distant-hybridization tissue cultured seedlings of cucumbers
InactiveCN103430851APromote reproductionImprove germination ratePlant tissue cultureHorticulture methodsWild speciesBud
The invention relates to a method for improving the domestication survival rate of distant-hybridization tissue cultured seedlings of cucumbers, belonging to the field of biotechnology. The method is mainly used for propagation and preservation and domestication seedling of hybridization materials of cucumber species and provides technical guarantee for application of a distant hybridization technology to cucumber breeding. The method mainly includes the steps of performing embryo rescue to obtain aseptic seedlings, carrying out induced differentiation on adventitious buds, carrying out regeneration bud extension and rooting culture and domestication transplanting, and regulating and controlling a specially prepared culture medium and external environment conditions. The method is simple in operation, has low requirements on raw materials and laboratory facilities, and has extremely good effects. According to the method, the obtained distant-hybridization tissue cultured seedlings have strong functional roots, the domestication period is shortened, and the survival rate of transplanting is improved. The method can provide a stable and high-efficiency regeneration seedling domestication system for hybridization of two individuals from different cucumber species and hybridization of two individuals from the same cucumis genus, and can be used for preservation and propagation and preservation of treasured cucumis wild species and transplanting seedling of common cucumber tissue cultured seedlings.
Owner:NANJING AGRICULTURAL UNIVERSITY
Method for improving hybridization success rate of sweet potato and incompatible material
InactiveCN104982328AIncrease success rateEasy to operatePlant tissue cultureHorticulture methodsMaterials preparationGrowth plant
The invention discloses a method for improving the hybridization success rate of sweet potato and an incompatible material. The method comprises the following steps: performing material preparation, hybridization treatment and plant growth regulator treatment; smearing the growth regulator on female parent stigmas and ovaries thereof after pollination, then continuing cultivation, and performing young embryo rescue and seedling cultivation. The method disclosed by the invention is higher in operability and high in practicability, the success rate of sweet potato inter-species incompatible hybridization can be obviously improved, and the method is also suitable for sweet potato intra-species incompatible hybridization.
Owner:SAAS BIOTECH & NUCLEAR TECH RES INST
Method for artificially synthesizing novel anti-clubroot brassica napus material
InactiveCN106035059AImprove inoculation efficiencyPlant tissue cultureHorticulture methodsSucroseSaccharum
The invention discloses a method for artificially synthesizing a novel anti-clubroot brassica napus material. The method includes: taking anti-clubroot brassica oleracea as a female parent to hybridize with anti-clubroot pakchoi; after 15 days of pollination of a generation F1, selecting seeds for embryo rescue, using a B5 fluid medium containing 2% of sucrose to culture immature seeds, and after immature embryos emerge and send up cotyledons, transferring the embryos to the B5 fluid medium containing 2% of sucrose, using 0.1% colchicines in 3-4-leaf stage to treat for 2h to realize doubling germination, cleaning, transferring into soil, and enabling selfing of synthesized plants by means of artificial pollination or saltwater spraying in a flowering stage; in a germination stage of selfing seeds, adopting a clubroot P4 physiological race for inoculation while taking an infected material as a contrast, inoculating at the same time, taking out seedling roots after 35 days, cleaning, observing whether lumps exist or not, and determining that the synthesized plants are resistant to clubroot if no lump exists. An effective approach is provided for breeding of anti-clubroot brassica napus strains, and clubroot inoculation efficiency is improved.
Owner:NORTHWEST A & F UNIV
Method for cultivating Lilium oriental hybrid embryos
InactiveCN102599053APrevent premature agingAddressing Post Fertilization BarriersPlant tissue cultureHorticulture methodsGermplasmPollination
The invention relates to a method for cultivating Lilium oriental hybrid embryos, which is effective in breeding effect and short in breeding period. The method includes: pollinating a Lilium oriental hybrid line; sectioning and peeling small embryos, ovules or ovaries; and performing isolated culture. By cross pollination of the ovaries and young embryos and isolated culture, the embryos can be taken out for culturing before abortion to avoid premature senility of distant hybrid embryos, and accordingly Lilium post-fertilization barrier can be overcome effectively, a great amount of distant hybrid embryos are allowed to continue growing into normal seeds, a breeding procedure is drawn close as soon as possible, and the breeding cycle is shortened. In addition, a great amount of distant hybrids are obtained, Lilium interspecific distant hybridization is achieved, and a batch of novel germplasm is created. The embryo rescue technology and the technology of within-bottle Lilium bulbing are combined, so that culture cycle of Lilium hybrids is shortened effectively, and hybrid survival rate is increased.
Owner:赵兴华
Novel method for obtaining artificially synthesized hexaploid wheat
InactiveCN102972284AExpanded genetic backgroundRich genetic diversityPlant genotype modificationBiotechnologyGermplasm
The invention relates to a novel method for obtaining artificially synthesized hexaploid wheat. The novel method comprises the steps of distant hybridization and obtaining the artificially synthesized hexaploid wheat. The distant hybridization includes that the distant hybridization is carried out with aegilops tauschii as a female parent and common wheat as a male parent, young embryo rescue is carried out through a hybrid embryo, and a test-tube plantlet is obtained; and the test-tube plantlet grows over a summer, manual doubling germination is carried out in November by means of colchicines, normal cultivation management proceeds, and aegilops tauschii-wheat octaploid seeds are obtained the next year. The steps of obtaining the artificially synthesized hexaploid wheat include that hybrid is carried out by means of the obtained aegilops tauschii-wheat octaploid seeds and tetraploid wheat with various good genes, and the hexaploid wheat is directly manually obtained. The novel method is easy to carry out, and the hexaploid wheat germplasm with a rich genetic background can be conveniently and manually synthesized.
Owner:HENAN UNIVERSITY
Method for obtaining plant graft chimaera progeny by embryo rescue
InactiveCN101828525AEasy to operateThe effect of embryo rescue is remarkableCultivating equipmentsHorticulture methodsNutrient solutionFilter paper
The invention discloses a method for obtaining a plant graft chimaera progeny by embryo rescue, which comprises the following steps of: (1) picking an ovary in 1 day before the immature embryo of a crucifer graft chimaera is aborted; (2) sterilizing and flushing the ovary; (3) placing the ovary on a culture dish paved with filter paper which is fully soaked in nutrient solution, dissecting one end of the ovary and then tearing the ovary along a ventral suture, selecting and cutting light green and glossy ovule, suspensor and ovary wall tissue connected with the suspensor and inoculating the three to a development culture medium, culturing the three under a dark condition at the temperature of 25 + / - 2 DEG C, and then culturing the three under light; (4) picking mature seeds, inoculating the seeds to a sprouting culture medium, and culturing the seeds under the dark condition at the temperature of 25 + / - 2 DEG C; (5) after the seeds are sprouted and grow main leaves, transferring the seedlings to a sub-cultured medium to perform culture, and then transferring the seedlings to a rooting culture medium to perform rooting; and (6) taking the routed seedlings out, transplanting the seedlings to a nutrition pot filled with matrix, hardening the seedlings for 6 to 12 days, and then transferring the seedlings to a field so as to obtain the plant graft chimaera progeny.
Owner:ZHEJIANG UNIV
Method for realizing distant hybridization of adzuki beans and rice beans by utilizing young embryo rescue
ActiveCN104221861AGuaranteed hybrid pod yieldAvoid abortionPlant tissue cultureHorticulture methodsPollinationAbortion
The invention discloses a method for realizing distant hybridization of adzuki beans and rice beans by utilizing young embryo rescue. The method comprises the steps of (1) preparation of materials, namely, selecting more early-matured rice bean material as female parent, selecting adzuki bean variety with excellent comprehensive characters as male parent, and selecting disease-free full grains for planting; (2) material planting; (3) cross pollination; (4) young embryo rescue; (5) hybrid seedling transplanting. According to the method, the more early-matured rice bean material is taken as the female parent, and the adzuki bean variety with excellent comprehensive characters is taken as the male parent, so that the obtained hybrid young embryos can directly grow into seedlings, the seedlings are good in growth and development and high in survival rate of transplanting, distant hybridization F1 plants can normally bloom and bear fruit at the adult-plant stage, and seeds of F2 can be harvested. After the method is used, a great deal of distant hybridization embryos can continue to develop into normal seeds, abortion of distant hybrid embryos can be effectively avoided, a great deal of distant hybridization filial generations can be obtained, and interspecific distant hybridization of the adzuki beans and the rice beans can be finally realized.
Owner:INST OF CEREAL & OIL CROPS HEBEI ACAD OF AGRI & FORESTRY SCI
Method of constructing clone groups of lily hybridization generation
InactiveCN102907313ASpeed up breedingGet a shorter period of timePlant tissue cultureHorticulture methodsAfter treatmentGreenhouse
The invention provides a method of constructing clone groups of lily hybridization generation. The method comprises the following steps of cutting stigmas of screened lily target parents, hybridizing and collecting; performing embryo rescue such as germinating, multiplying and heading; expanding propagation from one plant to hundreds of plants; vernalizing after bulblets grow; cultivating the bulblets to soil of a greenhouse after treatment; overwintering twice; digging the bulblets out before the third winter; and thus obtaining hundreds of the individual flowering clone groups. Due to the adoption of the method, the period is greatly shortened from eleven years to five years, so that the breeding speed of lily is increased, and the efficiency of breeding new varieties of lily is improved. The method is simple in process, has obvious effects of obtaining lily distant hybridization generation of the lily and quickly constructing hybrid clone groups, and the traits of all plants in the groups are good consistently.
Owner:FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
Biotechnological breeding method for obtaining antiviral seedless grapes
InactiveCN104988179APromote degradationHighly disease resistant and even immuneFermentationPlant tissue cultureBiotechnologyCoat protein
The invention discloses a biotechnological breeding method for obtaining antiviral seedless grapes. The biotechnological breeding method comprises the following steps: carrying out hybridization between different varieties of seedless grapes and embryo rescue to obtain grape zygotic embryos containing seedless genes; inducing grape somatic embryogenesis through the grape zygotic embryos; constructing RNAi antiviral plant expression vectors, and converting the RNAi antiviral plant expression vectors into agrobacterium tumefaciens; converting the grape somatic embryos sourced from the grape zygotic embryos by utilizing the agrobacterium tumefaciens, thereby obtaining regeneration plants. The biotechnological breeding method has the advantages that seedless grape embryo rescue technology and the transgenic technology are combined, embryoids which are sourced from seedless grape hybridization immature embryos and obtained through embryo rescue are taken as receptor materials, and the RNAi antiviral vectors containing the conserved gene segments of grape virus coat protein (CP) are utilized for genetic transformation of agrobacterium tumefaciens mediation, so that novel grape materials which are both antiviral and seedless can be obtained from the transformed regeneration plants, and then selectively breeding of the novel varieties can be realized.
Owner:ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
Method for achieving isolated embryos through radish and turnip intergeneric distant hybridization
InactiveCN102210259BHigh affinityPromote innovationPlant tissue cultureHorticulture methodsPollinationSeedling
The invention relates to a method for achieving isolated embryos through radish and turnip intergeneric distant hybridization. The method is characterized by sequentially comprising the following steps of: (1) preparing materials; (2) sowing; (3) bagging and hanging plates; (4) performing pollination; and (5) saving embryos. By the method, the radish and turnip intergeneric distant hybridization isolated embryos are obtained successfully; hybrid embryo seedlings are cultivated; and a new breeding resource is obtained.
Owner:INST OF ECONOMIC CROP HUBEI ACADEMY OF AGRI SCI
Medicinal wild rice embryo rescue rapid progeny propagation culture medium and method
ActiveCN106305420AHigh frequency of differentiated green budsLow costHorticulture methodsPlant tissue cultureSeedlingCulture mediums
The invention provides a medicinal wild rice embryo rescue rapid progeny propagation culture medium. The medicinal wild rice embryo rescue rapid progeny propagation culture medium comprises an induction culture medium, a differentiation culture medium and a rooting culture medium, wherein the formula of the induction culture medium is MS+2.4D1.0-3.0mg / l+6BA0.1-0.5mg / l; the formula of the differentiation culture medium is MS+IAA1.0-3.0mg / l+6BA2.0-4.0mg / l+KT1.0-2.0mg / l; and the formula of the rooting culture medium is 1 / 2MS+IAA0.02-0.5mg / l. The invention also provides a medicinal wild rice embryo rescue rapid progeny propagation method. According to the medicinal wild rice embryo rescue rapid progeny propagation culture medium and method provided by the invention, healthy and rapid growth of tissue culture seedlings can be realized a short period of time.
Owner:NINGBO ACAD OF AGRI SCI +1
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