Novel germplasm creating method by distant hybridization of Brassica campestris ssp. chinensis Makino and Raphanus sativus

A technology of distant hybridization and heading cabbage, applied in horticultural methods, botanical equipment and methods, plant genetic improvement, etc., can solve the problems of long time, unstable variation, etc. Effect

Inactive Publication Date: 2014-07-30
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0005] The purpose of the present invention is to aim at the above-mentioned traditional distant hybridization breeding method time is long, hybrid F 1 Unstable variation is prone to occur, and a new germplasm creation method for distant hybridization between non-heading Chinese cabbage and radish is provided

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  • Novel germplasm creating method by distant hybridization of Brassica campestris ssp. chinensis Makino and Raphanus sativus
  • Novel germplasm creating method by distant hybridization of Brassica campestris ssp. chinensis Makino and Raphanus sativus
  • Novel germplasm creating method by distant hybridization of Brassica campestris ssp. chinensis Makino and Raphanus sativus

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Embodiment Construction

[0026] Example The new germplasm creation method of distant hybridization between non-heading Chinese cabbage and radish, including:

[0027] The basic medium is MS medium; the following culture is based on sterilization at 121°C and 1.1Mpa for 15 minutes.

[0028] ①Ovary culture medium (Ⅰ): MS+0.5g / L hydrolyzed casein+0.8% agar, pH 5.8;

[0029] ② Embryo rescue medium (Ⅱ): MS+5% coconut milk+0.5g / L hydrolyzed casein+0.8% agar, pH 5.8;

[0030] ③Differentiation medium (Ⅲ): MS+2mg / L6-BA+0.1mg / L NAA+5% coconut milk+0.8% agar, pH 5.8;

[0031] ④ Subculture medium (Ⅳ): MS+0.2mg / L6-BA+0.02mg / L NAA+0.8% agar, pH 5.8;

[0032] ⑤ Rooting medium (Ⅴ): 1 / 2MS+0.2mg / LNAA+0.8% agar, pH 5.8.

[0033] (1) Pollination method: During the full flowering stage, use autotetraploid non-heading cabbage [4] (2n=4X=40) as the female parent, and autotetraploid radish [5] (2n=4X=36) As the male parent, put a sulfuric acid paper bag on the day before flowering, and use the repeated pollination method...

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Abstract

The invention discloses a novel germplasm creating method by distant hybridization of Brassica campestris ssp. chinensis Makino and Raphanus sativus. The method uses autotetraploid Brassica campestris ssp. chinensis Makino as a female parent and autotetraploid Raphanus sativus as a male parent to conduct artificial sexual crossbreeding; and repeated pollination and in vitro embryo rescue techniques are employed to obtain distant hybridization novel germplasm of autotetraploid Brassica campestris ssp. chinensis Makino and autotetraploid Raphanus sativus. The invention for the first time adopts sexual crossbreeding of the autotetraploid Brassica campestris ssp. Chinensis Makino and autotetraploid Raphanus sativus and in vitro embryo rescue technique to obtain new germplasm of distant hybridization. Compared with traditional diploid hybridization, which obtains zygotic embryo for ploidy reduplication, the method does not require chromosome doubling operation, and can significantly shorten the breeding time; and the hybrid F1 can reach stable inheritance. The novel germplasm created by the invention creation has the genetic materials of Brassica campestris ssp. chinensis Makino and Raphanus sativus, and a wider genetic diversity.

Description

technical field [0001] The invention belongs to the field of crop breeding and relates to a method for creating a new germplasm of non-heading Chinese cabbage and radish for distant hybridization. Background technique [0002] Non-heading cabbage (Brassica campestris ssp.chinensis Makino) is a plant of the genus Brassica in the family Brassicaceae. It is a popular vegetable that people love. It is widely planted in the south and also introduced and cultivated in large quantities in the north. The relevant departments often use it as a measure of the supply of vegetables. Main signs In recent years, Japan, the United States and some countries in Southeast Asia and Europe have also been widely introduced, and have gradually become a worldwide vegetable [1]. Radish (Raphanus sativus L.) is a plant of the genus Radish in the Brassicaceae family. It is one of the largest vegetable crops in my country and is also a worldwide vegetable. It has high food value and medical value [2]....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H4/00
Inventor 张蜀宁李英孙成振郑金双王雅美侯喜林
Owner NANJING AGRICULTURAL UNIVERSITY
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