56 results about "Family Brassicaceae" patented technology

The invention discloses isaria fumosorosea wettable powder as well as a preparation method and an application thereof. The isaria fumosorosea wettable powder comprises the following components by mass percent: 5%-20% of isaria fumosorosea conidiophore powder, 3%-5% of wetting agent, 3%-5% of dispersing agent, 0.1%-0.5% of ultraviolet protective agent, and the balance of carrier. The isaria fumosorosea is an isaria fumosorosea strain SCAU-IFCF02 which is preserved in China Center for Type Culture Collection on November 1, 2013 with the preservation number CCTCCNO: M2013526. The isaria fumosorosea wettable powder is mainly used for preventing and treating brassicaceous vegetable pests, has good effect in preventing and treating pests such as plutella xylostella and the like, and has the advantages of high insecticidal activity, stable effect, lasting effect, small impact on environment, and convenience in use.

The present invention relates to the production of γ-linolenic acid (18:3Δ6,9,12) or stearidonic acid (18:4Δ6,9,12,15) or γ-linolenic acid (18:3Δ6,9,12) and stearidonic acid (18:4Δ6,9,12,15) in transgenic plants of the family Brassicaceae, where the transgenic plants comprise at least 10% by weight of oleic acid based on the total fatty acid content and, as the result of the activity of the Δ6-desaturases used in the method, have an increased Δ6-C18-fatty acid content. The invention furthermore relates to novel nucleic acid sequences which code for the Δ6-desaturases used in the method, gene constructs comprising these nucleic acid sequences, a vector and transgenic plants comprising at least one nucleic acid sequence or a gene construct.

The invention relates to a biological agent. Active ingredients of the biological agent comprise Paenibacillus polymyxa N3-4. The invention is characterized in that the biological agent is aqua or wettable powder. The aqua comprises, by weight, 94.4% of five hundred million Paenibacillus polymyxa aqua, 0.5% of NNO, 5% of sodium dodecyl benzene sulfonate and 0.1% of sodium benzoate; and the wettable powder comprises, by weight, 40% of 50 hundred million Paenibacillus polymyxa N3-4 wettable powder, 7% of NNO, 3% of polyethylene glycol, 5% of sodium dodecyl benzene sulfonate and 45% of light calcium carbonate. During application, the aqua is used for root irrigation or the wettable powder is sprayed on roots so as to prevent and treat clubroot of cruciferous crops. The invention has the following advantages: through introduction of Paenibacillus polymyxa N3-4 which is safe to human and livestock and has strong inhibition effects on clubroot of cruciferous crops, usage of the biological agent for prevention and treatment of clubroot of cruciferous crops is realized; dosage of chemical agents and drug cost are reduced; at the same time, the ecological environment is improved, and chemical residues in cruciferous vegetable are reduced.

The invention discloses a plutella xylostella cotesia rubecula artificial propagation production method which comprises the following steps: collecting and propagating plutella xylostella cotesia rubecula species, producing hosts, conducting inoculation, propagation, development and feeding on plutella xylostella, conducting inoculation and cultivation on plutella xylostella cotesia rubecula, separating the plutella xylostella cotesia rubecula from the plutella xylostella, and conducting collecting, reserving and seed stock on plutella xylostella cotesia rubecula pupae. By means of the scheme of the plutella xylostella cotesia rubecula artificial propagation production method, advantaged plutella xylostella cotesia rubecula specie selection, propagation and pasturing technology is completed, high-quality, efficient and mass plutella xylostella cotesia rubecula propagation is achieved, mass production can be carried out, the plutella xylostella cotesia rubecula artificial propagation production method is applied to the technical field of biological control over agricultural pests, the plutella xylostella cotesia rubecula artificial propagation production method is used for preventing and treating primary pests such as the plutella xylostella on brassicaceous vegetables and meets production requirements of organic vegetables and green vegetables.

The invention relates to a fluorescent quantitative PCR detection technology of plasmodiophoromycetes, and its application. The technology is used for rapidly and accurately quantitatively detecting brassicaceous vegetable plasmodiophoromycetes. The technology comprises the following steps: extracting DNA from a sample, designing a specific primer, carrying out real-time fluorescent quantitative PCR detection on the ITS region gene fragment of the plasmodiophoromycetes by applying an SYBRGreen I based dye process fluorescent quantitative PCR technique, constructing a standard curve, and calculating the copy number of the ITS region gene fragment in the sample and the concentration of the plasmodiophoromycetes. The detection technology has the advantages of simple and rapid operation, strong specificity and high sensitivity, realizes a highest detection sensitivity reaching 24 copy numbers per reaction, and can also realize the analysis of large batch samples. The technology provides a good basis for the early-stage prediction forecasting of diseases, so the technology is adapted to be widely applied in the plant disease diagnosis detection field.

The invention discloses a pteromalus puparum venom Kazal-type serine protease Inhibitor PpSPI20 protein. The protein comprises an amino acid sequence shown as SEQ ID NO: 2. The invention further discloses genes coding the pteromalus puparum venom Kazal-type serine protease Inhibitor PpSPI20 protein. The genes comprise a nucleotide sequence at sites 70-246 in SEQ ID NO:1. The inhibitor disclosed bythe invention can be used for inhibiting activation of hemolymph PPO (Poly Phenylene Oxide) of imported cabbageworms or papilio xuthus. The invention further discloses a method for improving prevention ability of brassicaceous vegetables on lepidoptera pests. The method comprises the following steps: transforming brassicaceous vegetable cells by using the genes comprising the nucleotide sequenceshown as SEQ ID NO:1, and cultivating the transformed brassicaceous vegetable cells into plants.

A colorant of a plant belonging to the family Brassicaceae which has no or significantly reduced smell originating in the Brassicaceae plant, a colorant formulation containing this colorant, and a process for producing the odorless or almost odorless colorant of the Brassicaceae plant. This colorant can be prepared by treating an adsorption-treated Brassicaceae plant colorant extract by at least one method selected from among adsorption, ion exchange, acid treatment, and membrane separation to thereby give an aroma component concentration of 100 ppm or less in case where the color value E10%1 cm is 80.

The invention discloses artificially-synthesized feed added with vegetable ingredients for athetis dissimilis hampson and a feeding method of the artificially-synthesized feed, and belongs to the insect feeding technology. The feed is formed in the way that wheat bran and malt flour are used as main ingredients, and other auxiliary materials such as soybean flour, corn flour and peptone are added. The feed of a certain proportion is used for feeding the athetis dissimilis hampson, and brassicaceous vegetables are added in the feeding process so as to supplement nutrition. The average pupa weight of the athetis dissimilis hampson reaches 87.68 mg, the average survival rate of larvae is 93.8%, the percentage of pupation is 95.8%, the eclosion rate is 100%, and the average number of single-female oviposition is 763.95. Compared with natural feed (maize seedlings), the survival rate of the larvae, the pupa weight and the average oviposition quantity of the athetis dissimilis hampson on the aspect of artificial feed are improved obviously, the developmental duration of the larvae and the pupa duration are shortened remarkably, and no significant difference exists on the index aspects of the percentage of pupation, the eclosion rate, the oviposition earlier-stage condition and the like. Moreover, the feed cannot be affected by seasons, and the feed cannot contain residual components such as pesticide, either.

The invention discloses a Plutella xylostella (L) Enterococcus mundtii PxG1 bacterial strain and application thereof. The bacterial strain is collected in the GuangDong Microbial Culture Collection Center (GDMCC) on June 29th, 2020, and a culture collection number is GDMCC No: 61067. Research indicates that the eclosion rate of the Plutella xylostella (L) fed with the Enterococcus mundtii PxG1 bacterial strain is obviously lowered, and simultaneously indicates that the Enterococcus mundtii PxG1 bacterial strain has a function for improving Bt toxin insecticidal activity and carrying out synergy on Cry1Ac original toxins to quickly kill the Plutella xylostella (L), and the Enterococcus mundtii PxG1 bacterial strain can be used as a novel biological prevention and control bacterium, is usedfor preventing and controlling brassicaceae vegetable pests, and has good biological prevention and treatment potential and a good application prospect.

The invention relates to a lysobacter antibioticus HY strain and application thereof and belongs to the technical field of plant protection. The lysobacter antibioticus HY strain has already been preserved in the China General Microbiological Culture Collection Center on July 11, 2013 and the preservation number is CGMCC No.7912. The konjak rhizosphere bacteria lysobacter antibioticus HY strain is used for preparation of a medicine used for preventing and treating brassicaceous vegetable clubroots, tobacco black shanks and konjak bacterial soft rots in fields. The lysobacter antibioticus HY strain is efficient, non-toxic, safe, free of residue, capable of achieving the disease-control purpose by means of live bacteria, good in plant growth promoting effect, good in comprehensive performance, and suitable for industrialized production, bulk production and application.

The invention relates to a microbial secondary metabolite palmatine hydrochloride and application thereof and belongs to the technical field of biological pesticides. The secondary metabolite is prepared by the following steps: (a), producing a strain of L.antibioticus YFY02 with the collection number of CGMCC NO.2403; (b) culturing the strain YFY02 for two days by utilizing a KB culture medium, performing enlarged culture by adopting a conventional fermentation method, extracting the fermentation liquor by using ethyl acetate, and performing rotary evaporation, thereby obtaining a crude metabolite extract; (c) by utilizing liquid chromatography, Sephadex LH-20 gel column chromatography, mass spectrometry, nuclear magnetic resonance and other spectroscopic techniques, and separating, purifying and identifying the crude extract of the secondary metabolite; and (d) identifying to obtain the effective secondary metabolite palmatine hydrochloride. The invention also discloses application of the microbial secondary metabolite palmatine hydrochloride in the preparation of agents for preventing and controlling bacterial blight of rice, bacterial leaf streak and plasmodiophora brassicae woronin. The microbial secondary metabolite disclosed by the invention has the advantages of environment friendliness, low cost and excellent effects of preventing and controlling the rice bacterial diseases and plasmodiophora brassicae woronin.

The invention discloses a compound microbial agent and application thereof in preventing and treating various plant diseases. The compound microbial agent has a remarkable inhibition effect on variousmain soil-borne diseases such as bacterial wilt, root rot, soft rot, black shank, gibberellic disease and root black rot caused by bacteria, fungi and oomycetes; meanwhile, the biomass of solanaceaecrop tomatoes, brassicaceous crop brassica campestris and cucurbitaceae crop cucumbers is remarkably improved, and a remarkable inhibiting effect is also achieved on tomato yellow leaf curl viruses which are plant virus diseases. The compound microbial agent effectively solves the problems of environmental pollution and food quality caused by the fact that the conventional microbial agent only aims at single pathogenic bacteria and chemical pesticide prevention and treatment, and has a good application prospect in green and environment-friendly and agricultural sustainable development.

The invention relates to a method for breeding a brassica napus and mustard cytoplasm male sterility restoring line and belongs to the field of breeding of crops, which comprises the following steps of: carrying out intervarietal hybridization by using a mustard napus cytoplasm male sterile line WJS1A or a brassica napus and mustard cytoplasm male sterile line WNJ01A as a female parent and using green Chinese cabbages or Beijing cabbages as a male parent; selecting sterile plants with the characteristics of brassica napus from F2 to carry out selfing, carrying out test-crossing on the sterile plants serving as the male parent and the sterile line WJS1A to obtain male parent selfing seeds and test-crossing seeds and sowing the seeds; identifying the fertility of a test-crossing 1-generation in the flower season to eliminate a sterile combination and the male parent; selecting brassica napus plants which accord with the breeding aim from a fertile male parent line of a strong advantage combination to carry out selfing and carrying out test-crossing on the selected brassica napus plants and a brassica napus and mustard cytoplasm male sterile line; and repeatedly carrying out test-crossing for 3 to 4 generations until the brassica napus and mustard cytoplasm male sterility restoring line is bred. The method has very important significance of breeding the cytoplasm male sterility restoring lines of rapes and other cruciferae crops and utilizing the advantages of hybrids of the cytoplasm male sterility restoring lines of the rapes and other cruciferae crops.

The invention relates to an isothiocyanate-enriched vegetable condiment and a production method thereof, and belongs to the technical field of food processing. The isothiocyanate-enriched vegetable seasoning powder comprises cruciferae ripe vegetable powder and sprout vegetable powder thereof, wherein the weight ratio of the cruciferae ripe vegetable powder to the sprout vegetable powder is 1 to (1-5), and the content of isothiocyanate in the vegetable seasoning powder is 800-2500mg / 100g by dry weight. Specifically, cruciferous vegetables or sprout vegetables are used as raw materials of the isothiocyanate-enriched vegetable condiment. The isothiocyanate-enriched vegetable condiment is prepared through the following steps of performing selection, performing cleaning, performing heat treatment, performing freezing, performing enzymolysis, performing drying, and performing superfine comminution so as to obtain vegetable powder; and then compounding table salt, gourmet powder, garlic powder, fresh ginger powder, scallion powder, onion powder, chili powder, star aniseed powder and pricklyash peel powder, performing sterilization, and performing packaging so as to obtain the isothiocyanate-enriched vegetable condiment. The isothiocyanate-enriched vegetable condiment disclosed by the invention is simple in production technology, and reasonable and scientific in formula, and can be in industrialized production, the content of the isothiocyanate in the isothiocyanate-enriched vegetable condiment is 800-2500mg / 100g by dry weight, and the isothiocyanate-enriched vegetable condiment is an ideal health-care condiment.

The invention relates to a composition for controlling a diamondback moth of a brassicaceous vegetable and a preparation and application thereof. The composition contains two active components of chlorfenapyr(4-brombutyl-2-(4-chlorphenyl)-1-(ethoxymethyl)-5-(trichloromethyl)pyrrole-3-nitrile) and pyriproxyfen(4-phenoxylphenyl(RS)-2-(2-pyridyloxy)propyl ether), and the weight ratio of chlorfenapyr and pyriproxyfen is between 1:4 and 4:1. The composition preparation can be prepared from the composition with effective dosage, solid or liquid carriers, an auxiliary agent and other additives by a conventional method and used for processing the diamondback moth or a habitat thereof of the brassicaceous vegetable. The composition overcomes the defects of the prior art, expresses obvious synergy, can show optimal contact killing performance, stomach poisoning performance and ovicidal effect by processing the diamondback moth of the brassicaceous vegetable through applying smaller dosage, has long effect duration and simultaneously strengthens the rapid killing activity on the diamondback moth.

The invention relates to an eggplant cultural method for controlling eggplant verticillium wilt. The method comprises the following cultural steps of fertilization, ridge forming, selection of eggplant seedlings, planting and film mulching, interplanting of Cruciferae Brassica crops and field management. A degradation product, namely isosulfocyanide, of a secondary metabolism product (glucosinolate) in the Cruciferae Brassica crops is utilized to kill verticillium wilt pathogenic bacteria in soil, accordingly the incidence rate of the eggplant verticillium wilt is reduced and the yield of eggplants is improved.

A mosaic kohlrabi seed-keeping method comprises the steps of (1) sowing: soil working and sowing at the end of August; (2) transplanting: transplanting when the seedling age is 27-30 days, and removing the mutation when planting; (3) management: applying composite fertilizer when the yellow leaves fall off from the live seedling to be transplanted and new leaves grow, and spraying the seedling after transplanting by mixing 20 g imidacloprid with 15kg water; (4) re-planting: at the first ten days of December when dark frost comes, pulling out the mosaic kohlrabi and sunning it for one day; cutting off the leaves when planting on the second day, and transplanting it to the field edge or corner where white radish grows nearby; (5) harvesting: at the early June next year, pulling out the mosaic kohlrabi plant and putting it somewhere shelters from the rain; and threshing after ripening, shell removing, drying and collecting. According to the invention, the mosaic kohlrabi is pulled out for sunning for one day, then planted again, therefore the plant can grow from nutrition growth to reproduction growth; the mosaic kohlrabi is planted being mixed with white radish, therefore the anthesis can be postponed more than half a month, and the mosaic kohlrabi is prevented from mixed pollination with other cruciferous crops and avoiding biological hybrid.

The invention discloses a method based on genome size and for improving cruciferous crop characters. The method includes: S1, collecting germplasm resources of cruciferous crops, and measuring genomesize E; S2, subjecting genome size relative difference percent T to reciprocal crossing of 2-20% cruciferous crops to obtain F1 generation; S3, planting the F1 generation, and performing artificial castration; S4, using a rape dihaploid inducer to induce the F1 generation to obtain a Y1F2 generation; S5, planting the Y1F2 generation, selecting orderly single plants with normal fertility and ploidyfor selfing to obtain a Y1F3 generation; S6, planting the Y1F3 generation, identifying its stability, and analyzing normal distribution condition of genome size Eyx of the Y1F3 generation; S7, investigating target character of the Y1F3 generation; S8, selecting a strain with the target character and Eyx positioned within 3-5% of two ends of normal distribution as an improved breeding new material. The method has the advantages of short improving period, high efficiency and low cost.

The invention discloses a pteromalus puparum venom Kazal-type serine protease inhibitor protein PpSPI24 with an amino acid sequence as shown in SEQ ID NO:2. The invention further discloses a gene forcoding the chrysalis nasonia venom Kazal-type serine protease inhibitor protein PpSPI24, and the gene has nucleotide sequences of sites 70-231 in SEQ ID NO:1. The inhibitor can be used for inhibitingactivation of imported cabbageworm or papilio xuthus linnaeus hemolymph PPO. The invention further discloses a method of improving prevention force, on lepidoptera pests, of brassicaceous vegetables,and the method comprises the following steps: converting cells of the brassicaceous vegetables by the gene with the nucleotide sequences as shown in SEQ ID NO:1; and cultivating the converted cells ofthe brassicaceous vegetables into plants.

The invention relates to a brassicaceae crop cultivation mulch miniature greenhouse and application thereof. According to the brassicaceae crop cultivation mulch miniature greenhouse, furrows are arranged in trenches, seeding holes are formed in the bottoms of the furrows and covered with covering soil, mulch is laid on the upper edges of the furrows, and the mulch miniature greenhouse is formed by the space defined by the mulch, the covering soil and the side walls of the furrows. The brassicaceae crop cultivation mulch miniature greenhouse facilitates seed germination and growth under the severe cold and draught condition of the early spring, young seedlings can also be prevented from being burnt, and the brassicaceae crop cultivation mulch miniature greenhouse can be used for commercial breeding practice of late bolting of baby cabbages sown in spring.

The invention discloses a plutella xylostella serine protease inhibitor Serpin7 gene and an application thereof. A nucleotide sequence of the plutella xylostella serine protease inhibitor Serpin7 geneis shown as SEQ ID NO:1, and an amino acid sequence of the plutella xylostella serine protease inhibitor Serpin7 is shown as SEQ ID NO:2. After the recombinant protein Serpin7 is added for eating, the activity of plutella xylostella phenol oxidase PO can be strongly inhibited; after a polyclonal antibody anti-Serpin7 of Serpin7 is added for eating, the activity of phenol oxidase PO can be remarkably improved; and after RNAi silences the expression of the Serpin7 gene, the activity of plutella xylostella phenol oxidase PO is improved, and plutella xylostella larvae generate the strong melanization reaction, so that most plutella xylostella cannot normally pupate, and those which can pupate cannot successfully emerge. The result shows that the plutella xylostella Serpin7 is a negative regulatory factor of innate immunity in insects, can be used as a novel molecular target for biological prevention and treatment, is used for preventing and controlling cruciferous vegetable pests, and hasgood biological prevention and treatment potential and application prospect.

The invention provides a method for extracting plasmodiophora brassicae DNA of cruciferae vegetables and application of the method. A diatomite method is adopted to extract the DNA of a plasmodiophora brassicae sample of the cruciferae vegetables, the DNA of a plasmodiophora brassicae host is isolated, and a basis is laid for molecular studies of plasmodiophora brassicae. The method can be used for separating and detecting physiological strains of the plasmodiophora brassicae of the cruciferae vegetables, a specific molecular marker of the plasmodiophora brassicae is simultaneously used in a process of plant disease resistance identification, and the accuracy of specific disease resistance identification of the physiological strains can reach 100%. The method for extracting the plasmodiophora brassicae DNA of the cruciferae vegetables by using the diatomite method has good repeatability, high reliability and low detection cost and is time-saving and labor-saving.

The present invention relates to a process for purifying renewable feedstock comprising triglycerides, said process comprising the steps, where the renewable feedstock comprising triglycerides, comprising at least one plant oil originating from a plant of the family Brassicaceae, is treated with an aqueous medium to obtain a mixture, and a first stream comprising water and a second stream comprising triglycerides are separated from said mixture, and the second stream is obtained as purified renewable feedstock.

The invention discloses an agricultural plastic mulching film for controlling alternaria brassicicola of vegetables and a preparation method thereof. The preparation method comprises the following steps: a, extracting cellulose from plant straw, and pulping and refining to prepare micron cellulose; b, adding cobalt blue pigments into the micron cellulose, and uniformly mixing; c, impacting a mixture with high-pressure hot airflow for entwisting and combining closely fibers in fiber webs of the mixture, thereby forming a fiber web in which cobalt blue dyes are dispersed; d, preparing modified polyvinyl alcohol; e, heating and melting the fiber web and modified polyvinyl alcohol in an extruder, and performing plastic extrusion to obtain a blue film; and f, performing corona treatment and hotpress molding on the blue film, thereby obtaining the agricultural composite mulching film. A barrier layer in the double-layer composite mulching film disclosed by the invention is a blue layer capable of inhibiting alternaria brassicicola, and by virtue of the auxiliary dense fiber web, brassicaceous vegetables can be effectively prevented from being infected with alternaria brassicicola, the effects of maintaining heat and retaining water are achieved, and the film is excellent in mechanical property. The film has environmental-friendly and biodegradable properties.

The invention discloses an insecticidal composition containing furan tebufenozide, namely an insecticidal composition containing an active ingredient A and an active ingredient B. The active ingredient A is selected from furan tebufenozide, the active ingredient B is selected from any one of the following compounds: chlorfenapyr, diafenthiuron and metaflumizone, and the weight part ratio of the active ingredients A and B is 1:60-60:1. The composition can prevent and control various insect pests, has an obvious synergistic effect, expands the insecticidal spectrum, and has higher activities for plutella xylostella, pieris rapae, beet armyworm, tea lesser leafhopper on brassicaceous vegetables, apple trees and tea trees. The pesticide dose is reduced, pesticide residues on crops is reduced, and environmental pollution is reduced. The insecticidal composition is safe for human and livestock and has good environmental compatibility, and the insect pests are unlikely to generate resistance to drugs.