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Paenibacillus polymyxa N3-4 used for preventing and treating clubroot of cruciferous crops and application thereof

A polymyxa spore-like and cruciferous technology, which is applied to Paenibacillus polymyxa N3-4, the application field in the prevention and treatment of cruciferous clubroot, and achieves the effect of preventing and treating clubroot

Active Publication Date: 2014-03-26
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the use of Paenibacillus polymyxa in the prevention and treatment of cruciferous clubroot at home and abroad

Method used

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  • Paenibacillus polymyxa N3-4 used for preventing and treating clubroot of cruciferous crops and application thereof
  • Paenibacillus polymyxa N3-4 used for preventing and treating clubroot of cruciferous crops and application thereof
  • Paenibacillus polymyxa N3-4 used for preventing and treating clubroot of cruciferous crops and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Inhibitory Effects of Paenibacillus polymyxa N3-4 on Various Pathogenic Bacteria and Pathogenic Fungi

[0024] Since Plasmodium brassicae cannot be cultivated indoors, the screening of clubroot biocontrol bacteria mainly uses a variety of pathogenic bacteria and pathogenic fungi as indicator bacteria. After obtaining better biocontrol bacteria, evaluate the biocontrol bacteria’s effect on Antagonistic effect and biocontrol ability of clubroot

[0025] A. For testing pathogenic bacteria and pathogenic fungi:

[0026] Pathogenic bacteria including Ralstonia solanacearum, Bacterial spot of rice and Phytophthora amylovora were provided by the Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences. Use LB culture solution (10.0 g of peptone, 5.0 g of yeast powder, 10 g of NaCl, 1000 mL of distilled water) at 28°C and 150 rpm for 2 days, and dilute to 10 with sterile water. 6 cfu / mL, spare.

[0027] Pathogenic fungi include Fusarium wilt of tomato, Sheath ...

Embodiment 2

[0039] Inhibitory effect of Paenibacillus polymyxa N3-4 on germination of dormant spores of Clubroot

[0040] Test materials: clubroot of cabbage, collected from Huoshaoping Town, Changyang County, Yichang City, Hubei Province, and stored in a refrigerator at -20°C for later use. Paenibacillus polymyxa N3-4, after being activated on the LB plate, was inoculated into 50 mL of LB culture solution, cultured with shaking at 28°C and 150 rpm for 2 days, and set aside.

[0041] The inhibition of Paenibacillus polymyxa N3-4 on the germination of dormant spores of cabbage clubroot pathogen was determined by lichen red staining. Take out the root of cabbage clubroot from the refrigerator at -20°C, place it at 25°C for 2 days, and dilute the collected dormant spore liquid with sterile water to a concentration of about 10 5 A / mL. Add Paenibacillus polymyxa N3-4 bacterial liquid and Clubroot pathogen dormant spore suspension in a ratio of 1:1 to a sterilized Erlenmeyer flask, use steril...

Embodiment 3

[0048] Metabolic secretions of Paenibacillus polymyxa N3-4-production of antimicrobial-related substances

[0049] Test materials: protease detection medium: skimmed milk powder 100g, agar 20g, dilute to 1000mL. Chitinase detection medium: colloidal chitin 15g, MgSO4 7H 2 O0.5g, FeSO 4 ·7H 2 O0.01g, K 2 HPO 4 0.7g, KH 2 PO 4 0.3g, 20g agar, dilute to 1000mL, pH7.0~7.2. Cellulase detection medium: peptone 10g, yeast powder 10g, sodium carboxymethylcellulose 10g, NaCl 5g, KH 2 PO 4 1g, 20g agar, dilute to 1000mL, pH7.0. Siderophilic detection medium: CAS60.5mg, 10mL ferric iron solution (1mmol L -1 FeCl 3 .6H 2 O), HDTMA72.9mg, agar 20g, be settled to 1000mL, pH7.0.

[0050] The Paenibacillus polymyxa N3-4 screened by Jiangsu Academy of Agricultural Sciences was activated on LB medium, then transferred into 50 mL LB culture medium, cultured at 28°C and 150 rpm for 2 days with shaking, and set aside. Spot 5 μL of Paenibacillus polymyxa N3-4 bacteria solution in the ...

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Abstract

The invention relates to a biological agent. Active ingredients of the biological agent comprise Paenibacillus polymyxa N3-4. The invention is characterized in that the biological agent is aqua or wettable powder. The aqua comprises, by weight, 94.4% of five hundred million Paenibacillus polymyxa aqua, 0.5% of NNO, 5% of sodium dodecyl benzene sulfonate and 0.1% of sodium benzoate; and the wettable powder comprises, by weight, 40% of 50 hundred million Paenibacillus polymyxa N3-4 wettable powder, 7% of NNO, 3% of polyethylene glycol, 5% of sodium dodecyl benzene sulfonate and 45% of light calcium carbonate. During application, the aqua is used for root irrigation or the wettable powder is sprayed on roots so as to prevent and treat clubroot of cruciferous crops. The invention has the following advantages: through introduction of Paenibacillus polymyxa N3-4 which is safe to human and livestock and has strong inhibition effects on clubroot of cruciferous crops, usage of the biological agent for prevention and treatment of clubroot of cruciferous crops is realized; dosage of chemical agents and drug cost are reduced; at the same time, the ecological environment is improved, and chemical residues in cruciferous vegetable are reduced.

Description

technical field [0001] The invention relates to a biocontrol agent and its application, in particular to a strain of Paenibacillus polymyxa N3-4 and its application in preventing and treating cruciferous clubroot. Background technique [0002] Cruciferous clubroot, commonly known as root cancer, is a worldwide soil-borne disease caused by the infection of Plasmodiophora brassicae Woronin. It has a wide range of hosts and can harm Chinese cabbage, Chinese cabbage, cabbage, More than 100 kinds of cultivated and wild cruciferous plants such as radish, shepherd's purse, cauliflower, mustard greens and rapeseed. In recent years, the occurrence area of ​​cruciferous clubroot in my country has expanded year by year, and the severity has increased year by year. In my country, clubroot damages an area of ​​about 3.2-4 million ha per year 2 , accounting for more than 1 / 3 of the planting area of ​​cruciferous crops, and the area of ​​occurrence and damage in a pandemic year can reach...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N25/02A01N25/14A01P3/00C12R1/01
Inventor 刘邮洲梁雪杰乔俊卿杜艳
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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