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Method for breeding brassica napus and mustard cytoplasm male sterility restoring line

A cabbage type rape, male sterile line technology, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve problems affecting rape heterosis utilization, cytoplasmic single fertile pollen, etc.

Inactive Publication Date: 2012-07-04
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In view of the problem that the cytoplasmic male sterile line of Chinese rapeseed has a single cytoplasm and is prone to produce a small amount of fertile pollen at high or low temperature, it affects the utilization of heterosis in Chinese rapeseed

Method used

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  • Method for breeding brassica napus and mustard cytoplasm male sterility restoring line
  • Method for breeding brassica napus and mustard cytoplasm male sterility restoring line
  • Method for breeding brassica napus and mustard cytoplasm male sterility restoring line

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Breeding and Propagation of Brassica napus Cytoplasmic Male Sterile Restorer Line (Recovery 01)

[0021] (1) The cytoplasmic male sterile line WJS1A (2n=36, AABB) of mustard type rapeseed, the biological material was sent to the Chinese Type Culture Collection Center in Wuhan University, Wuhan City, Hubei Province for preservation on November 30, 2010, and its preservation No. CCTCC NO: P201018) is the female parent, and the cabbage variety Shanghai Qing or Beijing Chinese cabbage with the genome of AA is the male parent to carry out interspecific hybridization to obtain F 1 Hybrid(2n=28, AAB);

[0022] (2) F obtained in the seeding step (1) 1 Hybrid, Harvest F 1 free-pollinated F 2 seed;

[0023] (3) The F harvested in step (2) 2 Seeds planted in the field, in F 2 Fertile plants with the characteristics of Brassica napus were selected from the population for self-crossing, and used as testcross males to testcross the cytoplasmic male sterile line WNJ01...

Embodiment 2

[0029] Example 2: Breeding and Propagation of Restorer Line Hui 02 of Brassica napus Cytoplasmic Male Sterility

[0030] (1) Brassica napus mustard cytoplasmic male sterile line WNJ01A (2n=38, AACC, the biological material was sent to the Chinese Type Culture Collection Center in Wuhan University, Wuhan City, Hubei Province for preservation on November 30, 2010. The preservation number is CCTCCNO: P201019) as the female parent, and the cabbage variety Shanghai Qing or Beijing Chinese cabbage with the genome of AA as the male parent for interspecific crossing to obtain F 1 hybrid(2n=29, AAC);

[0031] (2) F obtained in the seeding step (1) 1 Hybrid, Harvest F 1 free-pollinated F 2 seed;

[0032] (3) The F harvested in step (2) 2 Seeds planted in the field, in F 2Fertile plants with the characteristics of Brassica napus were selected from the population for self-crossing, and used as testcross males to testcross the cytoplasmic male sterile line WNJ01A of Brassica napus, a...

Embodiment 3

[0038] Example 3: Comparison of the restoration and maintenance relationship of the restorer line of the present invention to various types of cytoplasmic sterility

[0039] During the flowering stage of Brassica napus, Hui01, Hui02, restorer lines 7-5, 91-Hui5900, 25CC1, 00N269c and three Brassica napus varieties Huashuang 4, Zhongshuang 9 and Huashuang 5 were selected, respectively. Testcross with WNJ01A CMS, Pol CMS, OguCMS, Kos CMS, and harvest F 1 mongrel. Will F 1 The hybrids were planted in the field for fertility identification at the flowering stage. The results of the determination of the relationship between the restorer line and the maintainer line are shown in Table 1, which shows that the restorer lines Hui01 and Hui02 have specific restoration of the cytoplasmic male sterile cytoplasm of Brassica napus.

[0040] Table 1 The results of determination of the relationship between four kinds of cytoplasmic sterile lines

[0041]

[0042] Note: (1) S in the tab...

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Abstract

The invention relates to a method for breeding a brassica napus and mustard cytoplasm male sterility restoring line and belongs to the field of breeding of crops, which comprises the following steps of: carrying out intervarietal hybridization by using a mustard napus cytoplasm male sterile line WJS1A or a brassica napus and mustard cytoplasm male sterile line WNJ01A as a female parent and using green Chinese cabbages or Beijing cabbages as a male parent; selecting sterile plants with the characteristics of brassica napus from F2 to carry out selfing, carrying out test-crossing on the sterile plants serving as the male parent and the sterile line WJS1A to obtain male parent selfing seeds and test-crossing seeds and sowing the seeds; identifying the fertility of a test-crossing 1-generation in the flower season to eliminate a sterile combination and the male parent; selecting brassica napus plants which accord with the breeding aim from a fertile male parent line of a strong advantage combination to carry out selfing and carrying out test-crossing on the selected brassica napus plants and a brassica napus and mustard cytoplasm male sterile line; and repeatedly carrying out test-crossing for 3 to 4 generations until the brassica napus and mustard cytoplasm male sterility restoring line is bred. The method has very important significance of breeding the cytoplasm male sterility restoring lines of rapes and other cruciferae crops and utilizing the advantages of hybrids of the cytoplasm male sterility restoring lines of the rapes and other cruciferae crops.

Description

technical field [0001] The invention relates to a breeding method for a cytoplasmic male sterile restorer line of Brassica napus mustard, belongs to the technical field of crop breeding, and is specially used for the breeding of a cytoplasmic male sterile restorer line of Brassica napus mustard and the production of hybrid seeds of strong dominant hybrid rapeseed . Background technique [0002] The use of cytoplasmic male sterile line, maintainer line and restorer line to produce hybrids is the main way to utilize rapeseed heterosis at home and abroad. Rapeseed cytoplasmic male sterile lines can be divided into two categories, one is homologous cytoplasmic male sterile, the male sterile cytoplasm mainly comes from natural mutations and hybridization between varieties, such as Brassica napus Pol CMS, Shan 2A CMS and Nap CMS, etc., their restorer lines are mainly obtained through test cross selection with varieties, so as to realize the matching of three lines. This type of ...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 吴江生石淑稳刘克德刘超王小峰
Owner HUAZHONG AGRI UNIV
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