176 results about "Test cross" patented technology

The invention relates to a method for automatically monitoring roadway deformation in real time. The method comprises the following steps of: distributing cross section marks on a selected cross section of a tested roadway section and distributing a plurality of observation mark points on the cross section; erecting a mining camera probe towards a monitored roadway in a mode that a main optical axis is vertical to the tested cross section; observing the initial true distance between previously-measured observation points, wherein the initial true distance is displayed as a display distance on a computer image; and acquiring a video image of the tested roadway section, monitoring the change of the image on monitoring center software of a computer in real time, processing the video image and automatically recognizing the cross section marks and the observation mark points so as to obtain a relative deformation value and an absolute deformation value in different statistics time scales or calculate the size and the change of the roadway cross section. The method has the advantages of automatic, real-time, continuous and non-contact monitoring, safety and high efficiency. A monitoring result can provide bases for the design of a roadway support, roadway maintenance and the prevention of rock burst and a roof disaster.

The invention relates to an automatic test device of the flatness and thickness of a metal sheet, which comprises a machine frame, a feeding mechanism, a test platform and a discharging mechanism. The machine frame and the test platform are connected through a Y-directional track. The test platform comprises a test frame, guide rails, a test cross beam, a cantilever fixing frame, a cantilever, an upper test probe, a lower test probe, a Y-directional ball screw mechanism and a measuring circuit. The automatic test device has the following beneficial effects that: the test is accurate; the test efficiency and the test precision are improved because the probes always cover the effective test range of a saw blade; mechanical and electrical integration technology is fully used, the structure is simple, the operation and maintenance are convenient, and the application in the field of the test of the flatness and thickness of other metals can be realized with a little improvement, and the promotion and use are easy; and an eddy induction principle is used to realize the test of the flatness and the thickness of the metal sheet, the interference of water and other non-metal impurities on the saw blade is eliminated effectively, precise positioning of the saw blade is not required in test, the price is low, and the operation is simple.

The breeding process of high-yield variety with green (black or yellow) shank and recessive white feather includes the following steps: hybridization between recessive white feather chicken or green (black or yellow) shank and recessive white feather chicken and high-yield brown-shell egg or white-shell egg chicken to obtain F1 generation hybrid individuals; selfing reproduction of F2 generation with the F1 generation and selecting green (black or yellow) shank and white feather chicken W; test crossing green (black or yellow) shank yellow chicken with W, selecting green (black or yellow) shank and pure white feather hem and cock and self-reproduction to produce Y; repeated test cross and selection to obtain recessive white feather chicken individuals; and self-reproduction to expand colony, constitute genealogy and obtain new chicken variety of high-yield variety with green (black or yellow) shank and recessive white feather.

The invention discloses a method for selecting and breeding a green-shin dominant white-feather broiler chicken strain and belongs to the technical field of molecular breeding. The marker-assisted selection method is adopted for identifying the genotype of the locus of dominant white feathers of chicken, the genotype of the locus can be quickly figured out, and the defects that test cross is complex, generation intervals are long and the like in conventional breeding are overcome. According to the green-shin dominant white-feather broiler chicken strain with the genotype II is produced by adopting the method, the complexity of test cross selecting and breeding is avoided, the generation intervals are shortened, the process of breeding is accelerated, and breeding cost is lowered. Moreover, the meat quality of the chicken strain is fine and tender, the taste of the meat is delicious, production performance is high, carcasses of the chicken do not have colored feather vestiges, and therefore the carcasses look better. Besides, the strain is used as a male parent to hybridize and match a high-yield laying hen breed or a fast-growth broiler chicken breed, and high-yield green-shin dominant white-feather broiler chicken or fast-growth green-shin dominant white-feather broiler chicken can be produced.

The invention relates to three-class cross breed paddy multifunctional restorer molecule assistant breeding selection method that includes the following steps: selecting wide compatible gene material and taking polymer cross breed, taking molecule mark assistant selection to excellent individual, taking anther culture of the excellent individual containing at least four different targets gene to gain excellent material, and gaining multifunctional restore series after taking test cross, repeating test cross. The invention supplies an actual method for selective cultivating new species.

The invention relates to a breeding method of typically sterile non glutinous rice widely compatible sterile line with high stigma exposure rate, belonging to the field of rice breeding. In the invention, indica type temperature sensitive sterile line Peiai 64S serves as a female parent, conventional non glutinous rice variety Xiushui 110 serves as a male parent, the two are hybridized, direction selection is carried out on later generations; single plant with good agronomic trait, high stigma exposure rate and early blossom time is selected, test cross is carried out on the single plant by typically sterile non glutinous rice sterile line Chunjiang 16A dwarfing-sterile, meanwhile, widely compatible testing seeds are used to carry out wide compatibility appraisal, thus breeding fine material Chunjiang 95 with high stigma exposure rate, early blossom time, good wide compatibility and capacity of staying sterile; Chunjiang 16A dwarfing-sterile / Chunjiang 95 F1 serves as the female parent, and Chunjiang 95 serves as the male parent to carry out back cross; fertility appraisal in a generation by generation manner and continuous back cross is carried out on the later generations after the back cross to transform the later generations after the back cross into typically sterile non glutinous rice widely compatible sterile line Chunjiang 95A with high stigma exposure rate and early blossom time, corresponding maintenance line thereof is Chunjiang 95B. The method of the invention has the advantages of improved fertility stability and hybrid non glutinous rice seed production output of non glutinous rice sterile line.

The invention discloses a method for cultivating a dominant-green-shin white feather broiler strain based on molecular assistant selection and belongs to the technical field of molecular breeding. According to the method for cultivating the dominant-green-shin white feather broiler strain based on molecular assistant selection, the method of molecular assistant selection is adopted for appraisal of a genotype of a broiler dominant white feather locus, so that the genotype of the white feather locus is rapidly judged, the dominant-green-shin white feather broiler strain with the II genotype is produced, complexity of test cross selective breeding is avoided, a generation interval is shortened, the breeding progress is accelerated, and therefore the cultivation cost is reduced. Meat of the dominant-green-shin white feather broiler strain cultivated through the method is fine and tender, taste of the meat of the broiler strain is delicious, the production performance of the broiler strain is high, color feather residues do not exist on a carcass, and therefore the carcass is beautiful. In addition, the strain can serve as a male parent and be hybridized with a high-yield laying hen strain or a fast-growing large broiler strain in a matched mode so that high-yield or fast-growing large dominant-green-shin white feather broilers can be produced respectively.

The invention discloses a method for breeding colored rice two-line hybrid rice. The method comprises the steps of carrying out hybridizing by taking colored rice +C as a male parent and a two-line sterile line S as a female parent, and carrying out backcrossing with the female parent to obtain BCnF2 generation seeds; selecting male sterile plants, carrying out paired test cross on the male sterile plants and a two-line restoring line R, and selecting and maintaining homozygous sterile line plants with colored seed coats and corresponding hybrid seeds; detecting seed heterosis, and selecting and maintaining hybrid plants and sterile line plants with heterosis to obtain colored rice sterile line seeds; identifying the fertility, yield, quality and resistance of the sterile line to obtain excellent colored rice sterile line plants; and breeding seeds to obtain a colored rice two-line hybrid system. According to the method, the colored rice and the two-line sterile line of the approved hybrid rice are hybridized and backcrossed, and the two-line sterile line plants with the colored seed coats and heterosis are screened through the restoring line, so that the obtained colored rice hybrid rice and the sterile line thereof not only have the colored seed coats, but also have the advantages of high yield, high quality and disease resistance.

The invention discloses a cultivation method of broad-spectrum durable blast resistance rice, comprising the following steps: 1 a rice-blast resistance germplasm gene bank construction; 2 a basic colony construction; 3 intercross-shuttle selection- intercross; 4 pedigree shuttle high-pressure selection; 5 test cross identification; 6. start-point temperature identification; 7 resistance spectrum measurement, the germplasm material with complete group resistance frequency of above 90% to the known rice blast pathogene is kept to be broad-spectrum durable blast resistance rice. The broad-spectrum durable blast resistance rice is generated using group improvement technique, thus the rice has broad-spectrum persistent rice-blast resistance and copes with new rice-blast physiological strain resistance-gene and solves biparental homologous resistance of hybrid rice and linkage problem of resistance gene and disadvantageous gene, thus the broad-spectrum durable blast resistance rice of good quality can be obtained and both production and income can be increased.

The present invention uses rice intermedium type sterile line as female parent, uses intermedius type restoring line as male parent for cross breeding rice hybrid species. Said invention also includes that uses hsien rice sterile line and intermedius sterile line as female parent and uses rice hsien-keng crossed duplex haploid (DH) population and hsien-keng crossed recombinant inbred line (RIL) population as male parent to make test cross to obtain four hybrid populations of hybrid F1 generation. By detecting character of F1 and using hisen-keng molecule specific probe to detect keng-type index of F1 correspondent to male parent DH and RIL the F1 character-male-type keng-type index correlation can be obtained.

Method for producing YY super-male and XY physiological female common carps, with which YY-chromosome super-male common carp can be cultivated and androgenetic YY super-male common carp is produced, where microsatellite markers are used for paternity testing and test crossing confirmation. The YY common carp is crossed with normal female common carp to produce the progenies of only male common carp, and without sex identification, the juvenile male common carp with known sex are subjected to artificial sex reversal to produce XY physiological female common carp.

The invention belongs to the technical field of poultry breeding, and relates to a method for cultivating a special male parent strain mainly used as yellow-feather broilers, which comprises the following steps of: 1, hybridizing to generate a F1 generation by taking a broiler chicken from another country as a male parent and a yellow-feather broiler with characteristics of jute feathers and yellow shins as a female parent, selecting and remaining F1-generation cocks and performing feeding and management according to a feeding and management method of breeding hens; 2, mating a F1-generation cock serving as the male parent with the female parent in the step 1 to generate a F2 generation, grouping respectively according to feather colors in the F2 generation, performing test cross, and performing the feeding and management according to the feeding and management method of the breeding hens; 3, crossing male and female individuals of each group obtained in the step 2 athwart and propagating, and purifying and fixing beneficial characters according to a family and individual selection method; and 4, breeding by four or more than four beneficial characters for generations to form a new broiler strain containing the bloodlines of the broiler chickens from other countries. By the method, the growth speed of the yellow-feather broilers in China is improved substantially, and the feed conversion rate is reduced.

The invention discloses a breeding method for a high-yield new variety line of recessive white-feather gooses and application thereof. The method comprises the following steps of: selecting the high-yield white-feather gooses without broodiness or with weak broodiness, and eliminating dominant white-feather individuals and keeping recessive white-feather individuals A by the test cross or the molecular marker method; self-reproducing the male individuals and female individuals of A to breed the recessive white-feather gooses B; and breeding B again, eliminating dominant white-feather homozygote and heterozygote, self-reproducing the recessive white-feather high-yield individuals with homozygous genotype to expand the group, building a family and breeding the family until the high-yield recessive white-feather goose new variety line C is bred, and performing dualistic or polyphyletic mating by taking the C as a female parent and a colour-feather local goose as a male parent to produce colour-feather gooses or simulated local gooses.

The invention discloses a method for breeding a recessive nuclear sterile temporary maintainer line by utilizing a Brassica napus microspore culture technology, which comprises the following steps of: crossing by taking a good variety / strain as a female parent and taking a recessive nuclear sterile temporary maintainer line as a male parent to obtain an F1 generation; then carrying out inducing culture on the isolated microspore of the plant of the F1 generation by a Brassica napus microspore culture technology, thereby obtaining a DH strain; carrying out self cross on the DH strain and carrying out test cross on the heat exchange and a sterile line, wherein the test cross variety is completely sterile, and the corresponding DH strain is the recessive nuclear sterile temporary maintainer line. The good temporary maintainer line with a good genetic background can be rapidly obtained by the method and is applied to Brassica napus recessive nuclear sterile cross breeding.

A method for culturing the high-quality meat chicken with green (or yellow) shin and dominant white feather includes hybridizing between local meat chicken and fast-growing white-feather meat chicken to obtain generation F1, selfing to obtain generation F2, choosing the green cyellow-spine white-feather chicken, test crossing with color-feather chicken, choosing deominant white-feather chicken, reproduction, enlarging clony, and crossing with moderm layer.

The invention discloses a method for selecting and breeding Brassica napus recessive genic-male-sterile (GMS) line through the microspore culture technology and the cloning technology. The method comprises the following steps: a fine variety / line is hybridized with a recessive GMS line or temporary maintaining line to obtain the F1 generation; then the Brassica napus microspore culture technology is adopted to perform inducing culture to the isolated microspores of the F1 generation plant and obtain a DH strain; and then the DH strain with the sterile character is test-crossed with the temporary maintaining line, 100% of the tester strain is sterile, the corresponding sterile DH strain is the recessive GMS line; and then the cloning technology is used to rapidly obtain a large number of plants of the sterile line which are used for the complete sterile line production. By using the method of the invention, the sterile line with stable and excellent genetic background can be rapidly obtained and used in the Brassica napus recessive GMS cross-breeding.

The invention discloses a preparation method and an application of a molecular marker of a rape male sterility restoring gene. The preparation method comprises the following steps of A, designing degenerate primers according to PPR gene conservative amino acid sites, B, extracting a lamina DNA of a rape cytoplasm male sterility restoring line through a cetyltrimethyl ammonium bromide (CTAB) method and magnifying the lamina DNA through a touchdown polymerase chain reaction (PCR) process to obtain a specific restoring gene candidate segment, C, carrying out recovery, conversion, sequencing and sequence analysis processes on PCR products, D, carrying out 5 ' RACE and 3 ' RACE processes and splicing a sequencing result to obtain a full-length cDNA sequence, and E, designing specific primers in 5 ' UTR and 3 ' UTR zones of the full-length cDNA sequence according to the full-length cDNA sequence to obtain a molecular marker. The invention also discloses an application of a restoring gene molecular marker in hybrid rape parent breeding. The preparation method has the advantages of improvements of a speed of restorer seeding and an efficiency of verification, simple and easy method, good operability, rapid identification speed of restoring genes, great reduction of necessary workload of test cross and progeny planting observation in a restorative verification process, and improvement of an efficiency of hybrid seeding.

The present invention discloses a method for breeding Brassica napus varieties and materials with a double haploid induction line of rapeseed, including: 1) determining target traits of breeding restorer lines, maintainer lines and conventional varieties of Brassica napus; 2) crossing or convergently crossing two or more Brassica napus with the target traits; 3) pollinating the cross or back-cross progenies with the double haploid induction line of rapeseed; 4) identifying the stability of the induced progenies; 5) performing test-cross identification or yield and resistance identification on the stable progenies; and 6) forming stable restorer lines and maintainer lines for cross breeding combination or for forming conventional varieties. The present invention can quickly and efficiently obtain rapeseed materials or conventional varieties with application value in breeding on a large scale, and lays a solid foundation for genetic breeding of Brassica napus, innovation of breeding resources, breeding of conventional rapeseed varieties, and breeding of new varieties of hybrid rapeseed. The method of the present invention can greatly improve the breeding speed and efficiency of hybrid or conventional varieties of Brassica napus, and reduce the human and material resources.

The present invention is cross breeding process of color trilinear cotton hybrid. Pigment gene is led into restoring line to be improved to breed color cotton restoring line with strong restoring capacity to fertility of cytoplasmic male sterile line. Pest and disease resisting gene and pigment gene are transferred into sterile line to breed color cotton cytoplasmic male sterile line with resistance to cotton blight, bollworm and pink bollworm separately. Through test cross and screening with color cotton type sterile line and white cotton restoring line or white cotton sterile line and color cotton restoring line, combined advantageous hybrrid F1 with high yield, high quality and high resistance is bred. The present invention can express the heterosis sufficiently.

The invention discloses a selective breeding method for a new variety of grain-saving green shank recessive white chickens and belongs to the technical field of molecular breeding. In the invention, by means of the molecular-assistant selection method, genotypes of chicken recessive white feather gene and sex-linked dwarf gene are identified, so that the genotypes can be quickly determined and thenew variety of grain-saving green shank recessive white chickens with genotype being cc / dwdw is produced. The method avoids complexness of test-cross selective breeding and reduces generation interval, and can breed the new variety of the grain-saving green shank recessive white chickens only after two generations, thus effectively reducing breeding cost. The grain-saving green shank recessive white chickens has small body size, consumes less feed, is low in basic metabolism and high in egg yield, has a delicious taste, is large in feeding density and high in feed conversion, can save cost, can be directly produced as commercial chicken and can be used in matching application.

The invention relates to a method for breeding a novel strain of anurous laying hens. The method includes steps of (1), selecting mutant anurous cocks and hens from indigenous chicken or laying hen resources and enabling the cocks and hens to perform self-reproduction to expand propagation; (2), selecting anurous cocks and hens from descendents of the expanded propagation, introducing blood relationship of high-yield laying hens into the anurous cocks and hens, selecting anurous cocks and hens in hybrid descendents, enabling the anurous cocks and hens to perform cross-breeding, and selectively reserving anurous cocks and hens in cross-bred descendents; (3), performing test-cross on the reserved anurous cocks and hens, evaluating the anurous cocks and hens and screening individual cocks and hens which are anurous genetic homozygous and enabling the individual cocks and hens to perform self-reproduction to expand propagation; (4), selecting the anurous genetic homozygous cocks and hens and improving the bodily form and appearance uniformity and the laying performance of the anurous genetic homozygous cocks and hens; (5), repeating executing the steps (3) and (4) until the novel strain of laying hens with the anurous character capable of being stably inherited. Appearance features of the anurous cocks and hens in cross-bred descendents meet requirements of breeding objectives. The method has the advantages that the bred novel strain of anurous laying hens is fast in growing speed and high in dressing percentage and egg numbers, and requirements of modern laying hen and broiler production can be met.

The invention provides a method for selecting and breeding a cabbage type rape cytoplasm sterile line through a rape doubled haploid inducible system. The method comprises the following steps: 1), determining restoring and maintaining relationships of stable strains; 2), according to test-crossing progeny trait specialization, performing backcross or multi-generation backcross on a temporary maintainer line and a stable sterile line; 3), pollinating the backcross progeny by using the rape doubled haploid inducible system; 4), identifying fertility of induced progeny; 5), selecting a perfect sterile single strain, and continuously using the rape doubled haploid inducible system to pollinate the sterile single strain; 6), after twice inducible pollinated progeny forms the sterile line, identifying the genetic stability and the sterility of the strain; 7), using the rape doubled haploid inducible system to pollinate, maintain, and select and breed stable new sterile lines with the maintainer line characteristics. According to the method provided by the invention, the cabbage type rape cytoplasm sterile line can be quickly selected and bred, and four generations can be obtained within two years at most; the genetic-stability cabbage type rape cytoplasm sterile line is obtained, the selecting and breeding speed of the cabbage type rape cytoplasm sterile line is greatly improved, the selecting and breeding speed and efficiency of the cabbage type rape hybrid variety are improved, and the labor and material investments are reduced.

The present invention relates to plant breeding technology, and is especially anther cultivation technological method of breeding unclear sterile dual purpose line and unclear substance sterile recovery line of capsicum. Extracorporeal anther cultivation technology is used in selective breeding of unclear sterile dual purpose line and unclear substance sterile recovery line of capsicum. That is, the dual purpose line is obtained through test cross of the fertile plant and sterile plant in contemporary anther cultivation offspring of dual purpose line hybrid, F1 selfing, test cross of similar fertile plant and sterile plant in the offspring lines and continuous two generation selection; and the recovery line is obtained through the test cross of the fertile plant and sterile plant in the contemporary anther cultivation offspring of three line hybrid with available sterile line, selectively breeding the F1 with fertility recovering rate of 100 % and stable fertility to obtain fertile plant as the corresponding recovering plant and final selfing.

The invention discloses an anthocyanin radish breeding method. The method comprises the following steps: (1) selecting a female parent and a male parent to hybridize; (2) continuously utilizing a sterile plant in a filial generation as the female parent to continuously back cross with the original male parent; (3) after back crossing for 3-4 generations, finding a sensitive sterile plant with a micro amount of pollen in a flowering period to carry out bud stage selfing; (4) taking a male sterile plant as the female parent to test cross with the original male parent; and (5) selecting a system with stable sterility, anthocyanin content being more than 160mg / 100g fresh weight, thioglycodise content being less than 95mg / 100g fresh weight, excellent comprehensive characters and good combining capability as a dominant male sterile line, and obtaining a back cross male parent which is a maintainer line. The anthocyanin radish bred by adopting the method has the anthocyanin content being more than 167mg / 100g fresh weight and the thioglycodise content being less than 90mg / 100g fresh weight; and the anthocyanin radish has the advantages of difficult hollowness, difficult inner flowering, disease resistance, bolting resistance and stable performance, so that anthocyanin can be conveniently extracted and processed.

The invention discloses a cucurbita pepo breeding method based on cold plasma treatment, and particularly relates to a method for obtaining cucurbita pepo with the functions of improving the seed germinating ability, increasing the height of seedlings, increasing the main root length, lowering a first female flower insertion node, improving the combining ability, antedating initial picking and increasing the biological yield. Collected cucurbita pepo seeds are planted and screened and are subjected to self-cross selective breeding to obtain cucurbita pepo parent materials, and seeds of the cucurbita pepo parent materials are subjected to the cold plasma treatment; the seeds of the cucurbita pepo parent materials subjected to the cold plasma treatment are subjected to excellent strain generation-adding selection, and an excellent variation self-cross line which has a target trait and is high in combining ability is selected by virtue of manners such as self cross, back cross and test cross; the excellent variation self-cross line is subjected to self-cross purification and rejuvenation to obtain stable male and female parents, and the male and female parents are hybridized to obtain an excellent cucurbita pepo hybridization assembly; by the combination of an anti-identification test and a variety comparison test, a new excellent cucurbita pepo variety is obtained finally.

The present invention discloses one kind of fast and efficient corn shuttle pedigree breeding process. By using American corn germ plasm 3163 as basic material and through four years of shuttle alternate planting in Sichuan Province in spring and summer and in Hainan Province in autumn and winter, excellent corn selfing line is bred. At the same time of selecting superior plants for selfing in Hainan Province, pollen and test variety parent 48-2 are test crossed; and via ecological comparison to select the excellent combination ZH002, regional test and production test for five years, outstanding corn hybrid Zhenghong-2 is bred. The shuttle breeding between the northern and the southern has fast breeding process and high selfing breeding efficiency.

The present invention relates to a method for breeding silkworm variety whose imaginal ala has no paraphyllium. Said breeding method includes the following several steps: parental selection, hybridization, backcross, test cross, continuous subculture and selfing so as to obtain two silkworm varieties whose imaginal ala has no paraphyllium.

A honglian-type hybrid japonica rice breeding method pertains to an application technique in the technical field of biology and modern agriculture, and is applicable to three-line japonica rice heterosis utilization. The honglian-type hybrid japonica rice breeding method comprises the following steps: honglian-type sterile cytoplasm of indica type rice is introduced to the japonica rice variety for transformation breeding of the honglian-type japonica rice sterile line; the honglian-type japonica rice sterile line is used for the test-cross screening or hybridization breeding of the honglian-type japonica rice restoring line; the honglian-type hybrid japonica rice is bred by selected mating of the honglian-type japonica rice sterile line and the honglian-type japonica rice restoring line. By breeding the honglian-type japonica rice sterile line and the corresponding restoring line to breed the honglian-type hybrid japonica rice, the honglian-type hybrid japonica rice breeding method solves the problem that the hybrid japonica rice BT sterile line in the existing production has unstable sterility and haploid cytoplasm and realizes three-line matching of the honglian-type japonica rice.

The breeding process of chicken variety with green (black or yellow) shank and recessive white feather includes the following steps: selecting mutant green (black or yellow) shank and white feather chicken from local green (black or yellow) shank yellow feather variety; self reproduction and selecting green (black or yellow) shank and pure white feather chicken individuals; test crossing the pure white feather chicken, eliminating dominant white feather chicken individuals and separating colored feather individuals; repeated the above said steps to stabilize genetic characteristic and to obtain new local chicken variety of high-yield variety with green (black or yellow) shank and recessive white feather.

The invention discloses a method for breeding Chinese cabbage temperature-sensitive sterile line and temperature-insensitive sterile line. The Chinese cabbage temperature-sensitive sterile line is hybridized with different target seed-parents; the hybridized generation is processed under low temperature during the bud stage; the hybridized generation with remarkable fertility conversion is chosen; during the fertility conversion period, Chinese cabbage homozygous temperature-sensitive male sterile line and the temperature-insensitive sterile line are cultivated by selfing of fertility recovery plant, test cross of the fertile plant and sterile plant and other procedures. Based on the genetic characteristics of Chinese cabbage temperature-sensitive sterility, the invention adopts manual low-temperature induction for processing, the breeded temperature-insensitive sterile line can settle the problem of micro-pollen of Chinese cabbage male sterility caused by temperature sensitivity; the breeded temperature-sensitive sterile line can establish Chinese cabbage temperature-sensitive male sterile double-duty lines, as a result, the process of utilization of male sterility is simplified; a new approach for making seeds by two-line method is created, and the male sterile genetic resources are further developed and utilized.