Method for breeding celery cabbage temperature-sensitive male sterile line and temperature-insensitive male sterile line

Abstract

The invention discloses a method for breeding Chinese cabbage temperature-sensitive sterile line and temperature-insensitive sterile line. The Chinese cabbage temperature-sensitive sterile line is hybridized with different target seed-parents; the hybridized generation is processed under low temperature during the bud stage; the hybridized generation with remarkable fertility conversion is chosen; during the fertility conversion period, Chinese cabbage homozygous temperature-sensitive male sterile line and the temperature-insensitive sterile line are cultivated by selfing of fertility recovery plant, test cross of the fertile plant and sterile plant and other procedures. Based on the genetic characteristics of Chinese cabbage temperature-sensitive sterility, the invention adopts manual low-temperature induction for processing, the breeded temperature-insensitive sterile line can settle the problem of micro-pollen of Chinese cabbage male sterility caused by temperature sensitivity; the breeded temperature-sensitive sterile line can establish Chinese cabbage temperature-sensitive male sterile double-duty lines, as a result, the process of utilization of male sterility is simplified; a new approach for making seeds by two-line method is created, and the male sterile genetic resources are further developed and utilized.

Description

technical field [0001] The invention belongs to the technical field of utilization of plant heterosis, and relates to a method for selecting a temperature-sensitive sterile line and a temperature-insensitive sterile line of Chinese cabbage according to the genetic law of the temperature-sensitive trait of male sterility in Chinese cabbage. Background technique [0002] The research and utilization of male sterile lines in Chinese cabbage is the demand for the utilization of heterosis. The utilization of heterosis in Chinese cabbage was first performed in Japan. In the 1960s, South Korea and China successively started breeding for heterosis in Chinese cabbage. With the development of heterosis breeding in Chinese cabbage, scientists from various countries have started research on male sterility in Chinese cabbage, breeding, innovation, dominant breeding and genetic laws. [0003] Japan, South Korea, etc. have carried out research on Chinese cabbage male sterile lines as early...

AI Technical Summary

Problems solved by technology

In 1979, the first dual-purpose line hybrid "Shenyang Kuaicai" (AB541×7216-43-4-1) passed the examination and approval, and the male sterile "dual-purpose line" was gradually applied as one of the methods for the first generation of cabbage hybrids. Since 50% of the fertile plants need to be pulled out for the reproduction of male sterile dual-purpose lines and the preparation of first-generation hybrid seeds, it is difficult to produce large-scale seeds, and the utilization of dual-purpose lines is rarely used at present.

[0004] In the 1980s, many scholars devoted themselves to the selection and breeding of cytoplasmic male sterility. For the Ogura radish cytoplasmic male sterile material imported from abroad, extensive backcross breeding and trait improvement were carried out on Chinese cabbage and Chinese cabbage; Chinese radish——Wangzhao red radish male sterile plant is used as the female parent to cross and backcross with 78-22-3 Chinese cabbage to obtain cytoplasmic sterile cabbage, but it also produces yellow leaves and low combining ability; at this stage, No substantial progress has been made in addressing defects such as leaf yellowing, growth retardation, nectary degeneration, and poor combining ability of radish sterile sources, so it has not been applied in production so far; in terms of rapeseed cytoplasmic male sterility, Shaanxi Provincial Vegetable Research Institute conducted a transgenic study on Chinese cabbage using the male sterile source of Brassica napus

[0006] At present, in the field of Chinese cabbage, there is no information on the selection and breeding of temperature-insensitive sterile lines that can overcome the phenomenon of micro pollen produced by temperature sensitivity, and temperature-sensitive sterile lines that can establish temperature-sensitive male sterile lines of Chinese cabbage to report

Images