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172results about How to "Individual big" patented technology

Culture method of hybrid new variety of Argopecten irradias and Argopecten purpuratus

The invention relates to a culture method of a new hybrid variety of Argopecten irradias and Argopecten purpuratus. The culture method comprises the following steps of fertilizing Argopecten irradias eggs with Argopecten purpuratus sperms to build multiple hybrid first filial generation lines, selecting excellent hybrid lines according to phenotype of each hybrid line, selecting excellent hermaphroditic individuals from the excellent hybrid lines, maturing the excellent hermaphroditic individuals, and then carrying out self-fertilization to build multiple selfing lines; selecting excellent lines from the selfing lines, selecting excellent hermaphroditic individuals from the excellent lines, carrying out selfing and selecting; and carrying out selfing for more than three generations according to the same method to obtain the new hybrid variety of Argopecten irradias and Argopecten purpuratus. The obtained new hybrid variety of Argopecten irradias and Argopecten purpuratus has stable inheritance, rapid growth, large size and wide temperature tolerance range. The culture method also can be implemented by adopting a colony culture method. Since the obtained new hybrid variety of Argopecten irradias and Argopecten purpuratus has the excellent characteristics of Argopecten irradias and Argopecten purpuratus at the same time, the new hybrid variety can be cultivated in Qingdao, Dalian and other northern sea areas and the yield is improved by more than 30% as compared with the yield of bay scallop.
Owner:QINGDAO AGRI UNIV

A specific antibody of major royal jelly protein MRJP1 and a preparation method thereof and Elisa quantitative detection thereof

The invention discloses a specific antibody of major royal jelly protein MRJP1 and a preparation method thereof and Elisa quantitative detection thereof. First, homology analysis is performed to amino acid sequences of proteins of all members of the Apismellifera major royal jelly protein MRJPs family (MRJP1-MRJP9) to select a specific polypeptide amino acid sequence unlike other MRJPs family members in MRJP1. The related specific polypeptide is synthesized by using a chemical method, and is used as an antigen to immunize New Zealand white rabbits; taking serum, and performing Elisa assay obtaining polyclonal antibody R2 with a relatively high titer, then purifying the antibody by using an affinity column prepared from the synthesized MRJP1 polypeptide. The titer of antibody R2 is detected via Elisa assay by using MRJP1 as the antigen, and the titer of the antibody is greater than 1:20000. The present invention provides a very reliable new rapid detection method for the qualitative and quantitative detection of MRJP1 in royal jelly, and also provides a very reliable technical means for quality control, freshness detection, and identification of genuine products of royal jelly and honey products for bee product quality supervision departments and processing and trading enterprises.
Owner:ZHEJIANG UNIV

Tilapia fry rearing method

The invention discloses a tilapia fry rearing method. The tilapia fry rearing method comprises the following steps: breeding parent tilapia fries which are bigger than nine-sieve, picking parent tilapias which are disease free, injury free, strong and good in sexual maturity to rear, hybridizing the female parent tilapias and with the male parent tilapias according to ratio of stocking, picking up roes, incubating the roes with flowing incubating water in an incubating tank, cultivating the roes by marking in bold, and cultivating fries. Oviposition period is shortened by about seven days, incubating rate is as high as 67.9%, fry survival rate is 89.6%, each female tilapia lays 6700 fries each year averagely, fry production rate is improved by 30%, and the sizes of the fries are uniform. By means of the tilapia fry rearing method, fries can be reared all through a year, a large amount of tilapia fries are reared and the tilapia fries grow fast and are strong in stress resistance. The tilapia fries reared in mass are born at the same time, gonad developments are synchronous, sizes are the same, characters are unified, and heredity purity is high. Moreover, the tilapia fry rearing method has the advantages that incubating period is short, incubating efficiency is high, inheritable characters are good, fertility is strong, and fries can be reared in an overwintering mode.
Owner:化州市光辉养殖场有限公司

Feed and management technique of Penaeus vannamei Boone

Cultured Penaeus vannamei Boone has the advantages of high growth speed, strong adaptability and high yield and becomes one of the main cultured varieties in northern areas of China. (1) pond water color control: the ideal color of water for culturing the Penaeus vannamei Boone is yellow green or yellow brown formed by green algae or diatoms; the algae or diatoms are benign biocenosis in the micro-ecological environment of a pond and play a role of purifying water quality; (2) control of pond water pH value, dissolved oxygen and transparency: the pH value suitable for the Penaeus vannamei Boone is 7.8-8.5, but the peak value of 9.2 is generated at the middle and final stages culturing, and therefore, the pH value control is especially focused in the culturing process; the pH value is not suitable to be high, otherwise, the toxicity of ammonia nitrogen can be increased, thereby restraining the growth of the Penaeus vannamei Boone; (3) scientific bait casting: generally, low-cost iced fish cream or small shells are fed, and some formula feeds are also fed; the formula feeds can be manually compounded; the formula feeds contain the following components: 17 percent of fish meal, 40 percent of soya-bean cake, 28 percent of bran, 10 percent of wheat middling, 3 percent of bone meal and 2 percent of additive; and (4) pond patrol: the pond is patrolled in the morning, at night and in the midnight every day to observe the water color change and whether Penaeus vannamei Boone raise above water to breathe or not; the activity condition, the growth condition and the repletion rate of the Penaeus vannamei Boone are observed by adopting two methods of lamplight observing and spoon-net inspection so as to regulate the bait casting amount and decide whether to start an oxygen-enriching machine or not.
Owner:刘瀚昌

Ecological circulation culture method and device for algae, prawns and sinonovacula constricta in winter

The invention discloses an ecological circulation culture method and device for algae, prawns and sinonovacula constricta in winter. The device comprises an alga pond, a high-position prawn culture pond, settling ponds, a sinonovacula constricta culture pond and purifying ponds, wherein a thermal-insulation plastic shed covers each pond; the high-position prawn culture pond is provided with a boiler. The method includes: inoculating micro-alga seed sources artificially cultured in the alga pond into the high-position prawn culture pond, the optical prawn culture temperature is guaranteed through boiler heat supply, micro-algae in prawn culture water passing the settling ponds is pumped into the sinonovacula constricta culture pond to serve as baits, and micro-alga seawater filtered by sinonovacula constricta is purified by the purifying ponds and pumped into the high-position prawn culture pond for recycling. The ecological circulation culture method and device has the advantages that the sinonovacula constricta can obtain sufficient micro-alga baits and appropriate growth water temperature in the whole culture period, and the high-position prawn culture pond is allowed to keep a relatively-stable water ecological environment system at the same time.
Owner:NINGBO UNIV

Bletilla striata rapid propagation and sunshine seedling strengthening method

The present invention relates to a bletilla striata rapid propagation and sunshine seedling strengthening method belonging to the technical field of bletilla striata artificial cultivation. The method comprises the following steps: a, seed induced germination culture, to be more specific, seeds taken from bletilla striata capsules are sowed onto a seed induced germination medium for culturing for 50 days to obtain seedlings; b, rooting culture, to be more specific, the seedlings are put on a rooting medium for culturing for 40 days at the temperature of 23-27 DEG C under the light intensity of 2000-2600Lux to obtain rooting seedlings; and c, sunshine seedling strengthening, to be more specific, the rooting seedlings are transferred to a sunshine greenhouses for culturing for 10 days at the temperature of 20-28 DEG C under the light intensity of 3000-4000Lux, and then the light intensity is adjusted to 5000-6000Lux for culturing for 20 to 25 days. The beneficial effects of the bletilla striata rapid propagation and sunshine seedling strengthening method is that: the bletilla striata rapid propagation and sunshine seedling strengthening method can greatly improve seedling and seed quality, the seedling and seed are thick and solid, and even, the planting survival rate is high, and the survival rate reaches over 95%.
Owner:INST OF AGRI ENVIRONMENT & RESOURCES YUNNAN ACAD OF AGRI SCI

Culture method of hybrid new variety of Argopecten irradias and Argopecten purpuratus

The invention relates to a culture method of a new hybrid variety of Argopecten irradias and Argopecten purpuratus. The culture method comprises the following steps of fertilizing Argopecten irradias eggs with Argopecten purpuratus sperms to build multiple hybrid first filial generation lines, selecting excellent hybrid lines according to phenotype of each hybrid line, selecting excellent hermaphroditic individuals from the excellent hybrid lines, maturing the excellent hermaphroditic individuals, and then carrying out self-fertilization to build multiple selfing lines; selecting excellent lines from the selfing lines, selecting excellent hermaphroditic individuals from the excellent lines, carrying out selfing and selecting; and carrying out selfing for more than three generations according to the same method to obtain the new hybrid variety of Argopecten irradias and Argopecten purpuratus. The obtained new hybrid variety of Argopecten irradias and Argopecten purpuratus has stable inheritance, rapid growth, large size and wide temperature tolerance range. The culture method also can be implemented by adopting a colony culture method. Since the obtained new hybrid variety of Argopecten irradias and Argopecten purpuratus has the excellent characteristics of Argopecten irradias and Argopecten purpuratus at the same time, the new hybrid variety can be cultivated in Qingdao, Dalian and other northern sea areas and the yield is improved by more than 30% as compared with the yield of bay scallop.
Owner:QINGDAO AGRI UNIV

Tissue culture quick propagation method of polygonatum kingianum

The invention discloses a tissue culture quick propagation method of polygonatum kingianum, which includes the steps of: 1) selecting and sterilizing explants; 2) inoculating the explants onto an induced differentiation culture medium, and culturing the explants for 40-50 days with the light intensity being 1800-2000 Lx, irradiating period being 8-10 h per day and culture temperature being 25-27 DEG C; 3) cutting the tissue, which has adventitious buds and projections, generated in the induced differentiation culture medium into dices, and inoculating the dices onto a proliferation culture medium to perform proliferation cultivation to achieve proliferation of adventitious buds; 4) cutting the buds, of which the cluster buds are not less than 0.5 cm in diameter and not less than 3 cm in height, obtained in the proliferation cultivation into single bodies with leaves cut away, inoculating the single bodies on a rooting culture medium, and culturing the single bodies for 30-35 days with the light intensity being 1800-2000 Lx, irradiating period being 10 h per day at the first 20 days and 12 h per day later, and culture temperature being 25-27 DEG C to obtain rooting seedlings; and 5) performing domestication and seedling hardening. The method is more than 95% in survival rate.
Owner:INST OF AGRI ENVIRONMENT & RESOURCES YUNNAN ACAD OF AGRI SCI

Ecological breeding method for mixed breeding of gold saddle rabbitfish and giant freshwater prawns

The invention relates to an ecological breeding method for mixed breeding of gold saddle rabbitfish and giant freshwater prawns. The ecological breeding method comprises the steps that 1, pond cleaning is performed before water inflowing, pond drying in the sun is performed for 14-16 days, and then water inflowing begins, wherein the salinity of a water body is 3-8; 2, the gold saddle rabbitfish and the giant freshwater prawns are bred in a breeding pond in a mixed mode, wherein the density of the gold saddle rabbitfish is 2500-2600 per mu, and the density of the giant freshwater prawns is 50000-5200p per mu; a seawater floating mixed feed and a giant freshwater prawn feed are fed respectively every day in month before the breeding process; only the seawater floating mixed feed is fed after one month of breeding. By adopting the breeding method, the gold saddle rabbitfish survival rate can be up to 85%, the yield of the gold saddle rabbitfish per mu is up to 192 kg, the yield of the giant freshwater prawn per mu is up to 200 kg, bred giant freshwater prawns are large and few get ill, a breeding effect is remarkable, and the ecological breeding method has a good application prospect.
Owner:EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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